AR017171A1 - A DESATURASA OF FATTY ACID, A CELL GUEST TO EXPRESS IT, THE USE OF SUCH CELL GUESTED, AN ISOLATED NUCLEIC ACID THAT CODIFIES IT, AN OIL OF THE SAME OBTAINED THROUGH SUCH CELL AND A COMPOSITIONAL FORMULA FORMULA , A SUPLE - Google Patents
A DESATURASA OF FATTY ACID, A CELL GUEST TO EXPRESS IT, THE USE OF SUCH CELL GUESTED, AN ISOLATED NUCLEIC ACID THAT CODIFIES IT, AN OIL OF THE SAME OBTAINED THROUGH SUCH CELL AND A COMPOSITIONAL FORMULA FORMULA , A SUPLEInfo
- Publication number
- AR017171A1 AR017171A1 ARP980104973A ARP980104973A AR017171A1 AR 017171 A1 AR017171 A1 AR 017171A1 AR P980104973 A ARP980104973 A AR P980104973A AR P980104973 A ARP980104973 A AR P980104973A AR 017171 A1 AR017171 A1 AR 017171A1
- Authority
- AR
- Argentina
- Prior art keywords
- acid
- cell
- host cell
- fatty acid
- host
- Prior art date
Links
Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Se describe una desaturasa de ácido graso capaz de catalizar la conversion de ácido oleico a ácido linoleico, ácido linoleico a ácido gama-linolenico oácido alfa-linolénico a ácido estearidonico, la cual ha sido codificada por una secuencia denucleo tidos seleccionada del grupo que consiste en los NO ID SEC:1, 3, 19, 21, 23, 25, 27, 28, 29, 30, 31, 32 y 33; y la cual comprende una secuencia de polipéptidos seleccionada del grupo que consiste en los NO. ID. SEC:2, 4, 5, 6, 7, 8, 9, 10,11, 20, 22 , 24, 26,34, 35, 36, 37, 38, 39, 4O y una secuencia de polipéptidos que comprende los residuos de aminoácidos 50-53, 39-43,172-176, 204-213 y 390-402 del NO. ID. SEC: 2; una célula huésped empleada para expresar la desaturasa de ácidograso preferent emente una célula Saccharomycesde levadura; y el uso de una célula huésped que expresa la desaturasa de ácido graso en un método destinado a la produccion de ácido gama-linolenico en uncultivo de células huesped, que comprende:desarrollar un cultivo huésped que tenga una multiplicidad de células huésped recombinantes, donde las células de lalevadura o un ancestro de las mencionadas células de la levadura se transformaron con un vector que comprende ADN fungal que codificaun polipéptido el cual convierte el ácido linolenico a ácido gama-linoleico, donde el citado ADN está operativamente asociado con una secuencia de control de expresion funcional enlas mencionadas células huésped, en condiciones por las cuales seexpresa dicho ADN, donde el ácido gama-linolenico se produce a partir de ácido linoleico enel mencionado cultivo huésped. También se describe el uso de una célula huésped que expresa la desaturasa de ácido estearidonico en un cultivo decélulashuésped; el uso de una célula hué sped que expresa la desaturasa de ácido graso en un método destinado a la produccion de ácido linoleico en un cultivo decélulas huesped; un ácido nucleico aislado el cual codifica la desaturasa de ácidograso, el uso de la célula huésped en un método destinado a la obtencion dela biosíntesis alterada del ácido graso poliinsaturado de cadena larga; un aceite o una fraccion del mismo se ha producido de acuerdo con este ultimo método;A fatty acid desaturase is described capable of catalyzing the conversion of oleic acid to linoleic acid, linoleic acid to gamma-linolenic acid or alpha-linolenic acid to stearidonic acid, which has been encoded by a denucleus sequence selected from the group consisting of the NO SEC ID: 1, 3, 19, 21, 23, 25, 27, 28, 29, 30, 31, 32 and 33; and which comprises a polypeptide sequence selected from the group consisting of NO. ID. SEC: 2, 4, 5, 6, 7, 8, 9, 10.11, 20, 22, 24, 26.34, 35, 36, 37, 38, 39, 4O and a polypeptide sequence comprising the residues of amino acids 50-53, 39-43,172-176, 204-213 and 390-402 of NO. ID. SEC: 2; a host cell used to express the fatty acid desaturase preferentially a Saccharomyces yeast cell; and the use of a host cell expressing fatty acid desaturase in a method intended for the production of gamma-linolenic acid in a host cell culture, comprising: developing a host culture having a multiplicity of recombinant host cells, where yeast cells or an ancestor of the aforementioned yeast cells were transformed with a vector comprising fungal DNA encoding a polypeptide which converts linolenic acid to gamma-linoleic acid, where said DNA is operatively associated with a control sequence of functional expression in said host cells, under conditions whereby said DNA is expressed, where gamma-linolenic acid is produced from linoleic acid in said host culture. The use of a host cell that expresses stearidonic acid desaturase in a mouse-grown culture is also described; the use of a host cell expressing fatty acid desaturase in a method intended for the production of linoleic acid in a host cell culture; an isolated nucleic acid which encodes the acid fatty acid desaturase, the use of the host cell in a method intended to obtain the altered biosynthesis of the long chain polyunsaturated fatty acid; an oil or a fraction thereof has been produced according to this last method;
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/US1998/007126 WO1998046763A1 (en) | 1997-04-11 | 1998-04-10 | Methods and compositions for synthesis of long chain polyunsaturated fatty acids |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR017171A1 true AR017171A1 (en) | 2001-08-22 |
Family
ID=45607465
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP980104973A AR017171A1 (en) | 1998-04-10 | 1998-10-06 | A DESATURASA OF FATTY ACID, A CELL GUEST TO EXPRESS IT, THE USE OF SUCH CELL GUESTED, AN ISOLATED NUCLEIC ACID THAT CODIFIES IT, AN OIL OF THE SAME OBTAINED THROUGH SUCH CELL AND A COMPOSITIONAL FORMULA FORMULA , A SUPLE |
Country Status (1)
| Country | Link |
|---|---|
| AR (1) | AR017171A1 (en) |
-
1998
- 1998-10-06 AR ARP980104973A patent/AR017171A1/en unknown
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| AR017171A1 (en) | A DESATURASA OF FATTY ACID, A CELL GUEST TO EXPRESS IT, THE USE OF SUCH CELL GUESTED, AN ISOLATED NUCLEIC ACID THAT CODIFIES IT, AN OIL OF THE SAME OBTAINED THROUGH SUCH CELL AND A COMPOSITIONAL FORMULA FORMULA , A SUPLE |
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