AR008364A1 - PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE - Google Patents

PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE

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Publication number
AR008364A1
AR008364A1 ARP990100680A AR008364A1 AR 008364 A1 AR008364 A1 AR 008364A1 AR P990100680 A ARP990100680 A AR P990100680A AR 008364 A1 AR008364 A1 AR 008364A1
Authority
AR
Argentina
Prior art keywords
procedure
recombinant human
oversighters
humanarecombinant
erythropoyetin
Prior art date
Application number
Other languages
Spanish (es)
Original Assignee
Bio Sidus S A
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bio Sidus S A filed Critical Bio Sidus S A
Priority to ARP990100680 priority Critical patent/AR008364A1/en
Priority to BR9917606-8A priority patent/BR9917606A/en
Priority to CO99069965A priority patent/CO5241324A1/en
Priority to DE69939268T priority patent/DE69939268D1/en
Priority to EP99958811A priority patent/EP1127063B1/en
Priority to PCT/US1999/026241 priority patent/WO2000027869A1/en
Priority to CA2350327A priority patent/CA2350327C/en
Priority to US09/830,964 priority patent/US7012130B1/en
Priority to JP2000581046A priority patent/JP4516692B2/en
Priority to CA2704598A priority patent/CA2704598A1/en
Priority to AU16100/00A priority patent/AU1610000A/en
Priority to AT99958811T priority patent/ATE403671T1/en
Priority to ES99958811T priority patent/ES2312219T3/en
Publication of AR008364A1 publication Critical patent/AR008364A1/en

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  • Peptides Or Proteins (AREA)

Abstract

Se refiere a un método para la obtencion de EPO humana recombinante de alto grado de pureza. El procedimiento reivindicado comprende, además,evitar exponer la proteína a condiciones extremas de temperatura y a solventes orgánicos que pudierana fectar su actividad biologica o resultar toxicos parasu uso en seres humanos. El procedimiento consiste en una precipitacion diferencial, una cromatografía de interaccion hidrofobica, concentracion ydiafiltracion, cromatografía sucesivaanion ica y cationica, nueva concentracion y diafiltracion y cromatografía de exclusion molecular. El procedimiento sedistingue por no utilizar pasos de cromatografía líquida de alta presion y por un alto porcentaje de recuperacion de EPO.Comprende t ambién a la EPOobtenida segun el procedimiento descripto.It refers to a method for obtaining high purity recombinant human EPO. The claimed procedure also includes avoiding exposing the protein to extreme temperature conditions and organic solvents that could affect its biological activity or be toxic for use in humans. The procedure consists of differential precipitation, hydrophobic interaction chromatography, concentration and diafiltration, successive ion and cation chromatography, fresh concentration and diafiltration and molecular exclusion chromatography. The procedure is distinguished by not using high pressure liquid chromatography steps and a high percentage of EPO recovery. It also includes the EPO obtained according to the described procedure.

ARP990100680 1998-11-06 1999-02-23 PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE AR008364A1 (en)

Priority Applications (13)

Application Number Priority Date Filing Date Title
ARP990100680 AR008364A1 (en) 1999-02-23 1999-02-23 PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE
BR9917606-8A BR9917606A (en) 1998-11-06 1999-07-07 Procedure for the purification of recombinant human erythropoietin from cell culture supernatants and recombinant human erythropoietin obtained with such procedure
CO99069965A CO5241324A1 (en) 1998-11-06 1999-11-05 PROCEDURE FOR PURIFICATION OF RECOMBINANT HUMAN ERYTHROPOYETIN FROM CELLULAR CROPS OVERRANKS. .
DE69939268T DE69939268D1 (en) 1998-11-06 1999-11-08 METHODS FOR CLEANING RECOMBINATIVE HUMAN ERYTHROPOIETIN FROM CELL CULTURAL RESEARCH
EP99958811A EP1127063B1 (en) 1998-11-06 1999-11-08 Methods of purifying recombinant human erythropoietin from cell culture supernatants
PCT/US1999/026241 WO2000027869A1 (en) 1998-11-06 1999-11-08 Methods of purifying recombinant human erythropoietin from cell culture supernatants
CA2350327A CA2350327C (en) 1998-11-06 1999-11-08 Methods of purifying recombinant human erythropoietin from cell culture supernatants
US09/830,964 US7012130B1 (en) 1998-11-06 1999-11-08 Methods of purifying recombinant human erythropoietin from cell culture supernatants
JP2000581046A JP4516692B2 (en) 1998-11-06 1999-11-08 Method for purifying recombinant human erythropoietin derived from cell culture supernatant
CA2704598A CA2704598A1 (en) 1998-11-06 1999-11-08 Methods of purifying recombinant human erythropoietin from cell culture supernatants
AU16100/00A AU1610000A (en) 1998-11-06 1999-11-08 Methods of purifying recombinant human erythropoietin from cell culture supernatants
AT99958811T ATE403671T1 (en) 1998-11-06 1999-11-08 METHODS FOR THE PURIFICATION OF RECOMBINATE HUMAN ERYTHROPOIETIN FROM CELL CULTURE SUPERNATIVES
ES99958811T ES2312219T3 (en) 1998-11-06 1999-11-08 METHODS TO PURIFY HUMAN ERYTHROPOYETIN RECOMBINANT OF CELLULAR CROP OVERRANKS.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ARP990100680 AR008364A1 (en) 1999-02-23 1999-02-23 PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE

Publications (1)

Publication Number Publication Date
AR008364A1 true AR008364A1 (en) 2000-01-19

Family

ID=45724369

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP990100680 AR008364A1 (en) 1998-11-06 1999-02-23 PROCEDURE FOR THE PURIFICATION OF RECOMBINANT HUMAN Erythropoietin FROM CELL CROP OVERSIGHTERS AND HUMANARECOMBINANT ERYTHROPOYETIN OBTAINED WITH SUCH PROCEDURE

Country Status (1)

Country Link
AR (1) AR008364A1 (en)

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