AP942A - Process for preparing 2- azadihydroxybicyclo[2.2.1]heptane compounds and the L - tartaric acid salt of the compound. - Google Patents

Process for preparing 2- azadihydroxybicyclo[2.2.1]heptane compounds and the L - tartaric acid salt of the compound. Download PDF

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AP942A
AP942A APAP/P/1999/001486A AP9901486A AP942A AP 942 A AP942 A AP 942A AP 9901486 A AP9901486 A AP 9901486A AP 942 A AP942 A AP 942A
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compound
mol
formula
heptane
azadihydroxybicyclo
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APAP/P/1999/001486A
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AP9901486A0 (en
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Michael K O'brien
Patrick Leon
Dennis Largeau
Matthew R Powers
Thierry Durand
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Aventis Pharmaceuticals Products Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
    • C07D491/12—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains three hetero rings
    • C07D491/18—Bridged systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • C07D209/52—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring condensed with a ring other than six-membered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
    • C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/55—Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Nitrogen Condensed Heterocyclic Rings (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Low-Molecular Organic Synthesis Reactions Using Catalysts (AREA)
  • Indole Compounds (AREA)

Abstract

A method according to the invention is directed to the preparation of a 2-azadihydroxybicyclo [2 .2 .1] heptane compound of formula (I) or (I') wherein * represents an R chirality, *' represents an S chirality, R is hydrogen or, respectively, a group of formula (II) or (II') wherein Rj. is alkyl and Ar is optionally substituted aryl, comprising bishydroxylating a bicyclo[2.2.1]heptane compound of formula (III) or (III') wherein *, *' and R are as previously defined, in the presence of about 0.1 mol % to about 5 mol % of a metal osmate compound or about 0.06 mol % to about 0.07 mol % osmium tetroxide, and an oxidizing agent capable of regenerating osmium tetroxide. The invention is also directed to the treatment of the (1R) diastereomer of the 2-azadihydroxybicyclo [2.2.1] heptane compound (I) wherein R is a group of formula (II) with L-tartaric acid, and the L-tartaric acid salt product thereof. Furthermore, the invention is directed to using the (1R) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound in an acid facilitated acetalizing or ketalizing reaction that results in the protection of the dihydroxy moieties thereof in isopropanol. In addition, the invention is directed to oxidizing a bis O-protected derivative of the (1R) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound to a corresponding lactam compound in the presence of about 0.01 mol % to about 1 mol % of Ruo2 with about 3 equivalents of an oxidant to form the lactam compound with an enantiomeric excess ("ee") of greater than or equal to about 95%.

Description

PROCESS FOR PREPARING 2-AZADIHYDROXYBICYCLO[2.2.1]HEFTANE COMPOUNDS AND THE L-TARTARIC ACID SALT OF THE COMPOUND
This application is a continuation-in-part of U.S. patent application Serial No. 08/655,395, filed May 30, 1996, which in turn is a continuation-in-part of U.S. patent application Serial No. 08/476,156, filed June 7, 1995.
Field of the Invention
The present invention is directed to a method for preparing a
2-azadihydroxybicyclo[2.2.1]heptane compound. The invention is also directed to an L-tartaric acid salt of the (IR) diastereomer of the 2-aza-dihydroxybicyclo[2.2.1]heptane compound and its preparation. In addition, the invention is directed to a method for bis O-protection of the (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound and a method for oxidizing derivatives of the (IR) diastereomer of the 2-azadihydroxy-bicyclo[2.2.1]heptane compound to a corresponding lactam compound.
U.S. Patent No. 5,284,769 discloses that a lactam compound, which encompasses a lactam compound prepared according to the invention, as being a useful synthon for preparing pharmaceutically active agents. J. Chen et al., Tet. Lett., 30 5543 (1989) disclose a lactam compound, which encompasses a lactam compound prepared according to the invention, as being used in preparing a compound that is active as an adenosine agonist.
Reported Developments
C. K.-F. Chui, Syn. Comm.. 26(3), 577 (1996) discloses resolving a diastereomeric mixture of bicycloheptenamine compounds of formulae (i) and (ii)
AP/P/ 9 9 / 0 1 4 8 6 using L-dibenzoyl tartaric acid, i.e., by fractional crystallization. The Chui reference does not disclose means for resolving bishydroxylated products of tine diastereomeric mixture.
S. J. C. Taylor et ah, Tetrahedron: Asymmetry, 4(6), 1117 (1993) disclose the enzymatic resolution of a lactam of formula (iii)
AP 00942
'-' (iii) to yield the enantiomers of formulae (iv) and (v)
S. J. C. Taylor et al. do not disclose any means for resolving bishydroxylated products of the lactam (iii).
U.S. Patent No. 5,284,769 discloses the enzymatic resolution of a lactam of formula (vi)
•to yield the enantiomers of the lactam. U.S. Patent No. 5,284,769 does not disclose any means for resolving bishydroxylated products of the lactam (vi).
SUMMARY OF THE INVENTION
A method according to the invention is directed to the preparation of a 20 2-azadihydroxybicyclo[2.2.1]heptane compound of formula
K 0 / 6 6/d/dV wherein * represents an R chirality, *’ represents an S chirality, R is hydrogen or, respectively, a 25 group of formula
AP 00942
or
Ri
Ar (ip) wherein R, is alkyl and Ar is optionally substituted aryl, comprising bishydroxylating a bicyclo[2.2.1]heptene compound of formula
wherein *, *’ and R are as previously defined, in the presence of about 0.1 mol % to about 5 mol % of a metal osmate compound or about 0.06 mol % to about 0.07 mol % osmium tetroxide, and an oxidizing agent capable of regenerating osmium tetroxide.
The invention is also directed to the treatment of the (IR) diastereomer of the 2azadihydroxybicyclo[2.2.1]heptane compound (I) wherein R is a group of formula II with L-tartaric acid, and the L-tartaric acid salt product thereof. Furthermore, the invention is directed to using the (IR) diastereomer of the
2-azadihydroxybicyclo[2.2.1]heptane compound or salt thereof in an acid facilitated acetalizing or ketalizing reaction that results in the protection of the dihydroxy moieties thereof in isopropanol. In addition, the invention is directed to oxidizing a bis O-protected derivative of the (IR) diastereomer of the
2-azadihydroxybicyclo-[2.2.1]heptane compound to a corresponding lactam compound in the presence of about 0.01 mol % to about 1 mol % of RuO2 or hydrate thereof with about 3 equivalents of an oxidant to form the lactam compound in an enantiomeric excess (“ee”) of greater than or equal to about 95%.
DETAILED DESCRIPTION OF THE INVENTION
As used above, and throughout the description of the invention, the following terms, unless otherwise indicated, shall be understood to have the following meanings.
“Alkyl” means an aliphatic hydrocarbon group which may be straight or branched having 1 to about 4 carbon atoms. Exemplary alkyl groups include methyl, ethyl, z'-propyl and i-butyl.
“Optionally substituted methylene” means -CH2- or that moiety wherein the hydrogen atoms are replaced individually by one or two groups, which may be identical or different, selected from alkyl or phenyl, or replaced concomitantly to form, together the carbon atom of the methylene, cycloalkyi.
AP/P/ 9 9/01486
ΑΡ Ο Ο 9 4 2 “Aryl” means optionally substituted phenyl or optionally substituted a- or β-naphthyl. A substituted aryl is substituted by one or more aryl group substituents, which may be identical or different, which include halo, alkyl, alkoxy, and nitro.
“Alkoxy” means an alkyl-O- group wherein the alkyl group is as previously described. Exemplary alkoxy groups include methoxy, ethoxy, i-propoxy and z-butoxy.
“Cycloalkyl” means an aliphatic cyclic ring of about 5 to about 6 carbon atoms. An exemplary cycloalkyl group is cyclohexyl.
“Acyl” means an alkyl-CO- group wherein the alkyl group is as previously described. Exemplary acyl groups include acetyl and propanoyl .
“Aroyl” means an aryl-CO- group wherein the aryl group is as previously described. An exemplary aroyl group is benzoyl.
“Halo” means fluoro, chloro, bromo or iodo. Preferred are fluoro and chloro.
“Oxidizing agent capable of regenerating osmium tetroxide” means an oxidant that will oxidize the osmium of the metal osmate (Os*6) to osmium tetroxide (Os*8) or reoxidizes the osmium tetroxide reduced in effecting the bishydroxylation to osmium tetroxide (Os*8). Examples of oxidizing agents capable of regenerating osmium tetroxide include N-methylmorpholine oxide or triethylamine oxide and potassium ferricyanide (K3FeCN6), preferred is N-methylmorpholine oxide.
“Metal osmate” means a salt compound formed from M”*, a metal cation wherein n is 1 or 2, and an osmium oxide anion complex [OsO4]'2, or hydrates thereof. Preferable metal osmates are alkali or alkaline earth osmates, including sodium, potasium, rubidium, cesium, calcium and barium osmates, more preferable is K2OsO4*2H2O. Examples of methods useful for preparing metal osmates are described by Β. N. Ivanov-Emin et al., Zh. Neorg. Khim. 31(5) 1238 (1986), H. C. Jewiss, J.C.S. Dalton Trans. 199 (1985), Β. N. Ivanov-Emin et al., Zh. Neorg. Khim. 29(4) 1241 (1984), Β. N. Ivanov-Emin et al., Zh. Neorg. Khim. 28(5) 1246 (1983).
“Salt thereof’ means the compound with a basic moiety neutralized by an acid to form the corresponding acid addition salt. Acids which can be used to prepare the acid addition salts include preferably those which produce, when combined with the free base, pharmaceutically acceptable salts, that is, salts whose anions are non-toxic to a patient, and so that subsequent use of the acid addition salt does not proscribe the salt from subsequent chemical reactivity. The acid addition salt is useful
AP/P/ 9 9/01486
AP 00942 for example as a source for the regeneration of the base compound therein by treatment with a base such as alkali, for purposes of purification and/or identification, or for interconversion to another acid addition salt form by an ion exchange procedures. Examples of acid addition salts include those encompassing the following acids: mineral acids such as hydrobromic, hydrochloric acid, sulfuric acid, phosphoric acid and sulfamic acid; and organic acids such as acetic acid, citric acid, lactic acid, tartaric acid, dibenzoyltartaric, malonic acid, succinic, 2,3-dimethoxysuccinic, methanesufonic acid, ethanesulfonic acid, benzenesulfonic acid, p-toluenesulfonic acid, cyclohexylsulfamic acid, quinic acid, and the like.
Preferred Embodiments
A special embodiment for the bishydroxylation method according to tire invention, is that wherein R is a group of formula II or ΙΓ.
A preferred embodiment for the bishydroxylation method according to tire invention, is that wherein R, is methyl or ethyl, and Ar is optionally substituted phenyl, which when substituted is substituted by one or more methyl or methoxy.
A more preferred embodiment for the bishydroxylation method according to the invention, is that wherein R, is methyl, and Ar is phenyl.
A preferred embodiment for effecting the bishydroxylation uses the osmium tetroxide at about 0.06 mol % to about 0.07 mol %, more preferably at about 0.06 niol %.
AP/P/ 99/y1486
Another preferred embodiment for effecting the bishydroxylation uses the metal osmate at about 0.1 mol % to about 5 mol %, more preferably at about 0.2 to about 0.5 mol %.
Yet, another preferred embodiment for effecting the bishydroxylation uses an alkali or alkaline earth osmate as the metal osmate, more preferably K,OsOj*2H2O.
A special embodiment for preparing the L-tartaric acid salt of the (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound, i.e.,
HO
HO
N—R · L-tartaric acid salt according to the invention, is that wherein R is a group of formula II.
A preferred embodiment for preparing the L-tartaric acid salt of the
AP 00942 (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound according to the invention, is that wherein R, is methyl and Ar is phenyl.
Another special embodiment according to the invention is for preparing the L-tartaric acid 5 salt of the (IR) diastereomer of the 2-azadihydroxybicyclo-[2.2.1]heptane compound in a substantially enantiomerically purified state in the presence of a (IS) diastereomer of the 2azadihydroxybicyclo[2.2.1 ]-heptane compound.
A preferred embodiment for preparing the L-tartaric acid salt of the 10 (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound according to the invention, is that wherein the preparation occurs in an aqueous-organic solvent mixture.
A more preferred embodiment for preparing the L-tartaric acid salt of the (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound according to the invention, is that wherein the organic solvent is isopropanol (IPA).
An even more preferred embodiment for preparing the L-tartaric acid salt of the (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound according to the invention, is that wherein the preparation occurs in an aqueous-IP A solvent mixture having a volume ratio of about 30:70 to about 15:85, further preferably of about 25:75.
A special embodiment for the acid facilitated acetalizing or ketalizing reaction method according to the invention, is directed to a method for preparing a compound of formula IV
AP/P/ 9 9 / 0 1 4 86 wherein R is as defined herein, R3. and R3„ are hydrogen, alkyl or phenyl, or R3, and R3.. taken together with the carbon atom to which they are attached form a cycloalkyl, comprising acid facilitated acetalizing or ketalizing of a compound of formula V (V)
AP 00942
Ί wherein R4. and R4.. are alkoxy, or taken together with the carbon atom to which they are attached form carbonyl, with the (IR) diastereomer of the 2-aza-dihydroxybicyclo[2.2.1]heptane as described herein or salt thereof in IPA.
A preferred embodiment for the acid facilitated acetalizing or ketalizing reaction is that wherein R4. and R4.. are methoxy and R3. and R3.. are methyl.
Another preferred embodiment for the acid facilitated acetalizing or ketalizing reaction is that wherein the acid facilitation is effected using trifluoroacetic acid (TFA).
Yet another preferred embodiment for the acid facilitated acetalizing or ketalizing reaction is that wherein R is a group of formula II.
A more preferced embodiment for the acid facilitated acetalizing or ketalizing reaction is that 15 wherein R, is methyl and Ar is phenyl.
Still another preferred embodiment for the acid facilitated acetalizing or ketalizing reaction is that wherein the (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound is in the form of an L-tartaric acid salt thereof.
A special embodiment for the lactam preparative method according to the invention, is . directed to a method for preparing a lactam compound of formula VI
(VI)
AP/P/ 9 9 / 0 1 4 8 6 wherein R’, and R”t independently are acyl or aroyl, or taken together form an optionally substituted methylene, and G, is hydrogen or an amino protecting group, comprising oxidizing a bis O-protected (IR) diastereomer of the 2-aza-dihydroxybicyclo[2.2.1]heptane compound of formula VII
(VII)
AP Ο Ο 9 4 2 in the presence of about 0.1 mol % to about 1 mol % of RuO2 or hydrate thereof with about 3 equivalents of an oxidant to form the lactam compound with an ee of greater than or equal to about 95%.
A preferred embodiment for the lactam preparative method is that wherein the RuO2 is present at about 0.5 mol %.
Another preferred embodiment for the lactam preparative method is that wherein the lactam compound is formed with an ee of greater than or equal to about 99%.
General parameters for the preparative methods are those described heretofore and below.
In general, the bishydroxylation is carried out under the conditions described by V. VanRheenen et al., Tetrahedron Letters, Vol. 23, 1973-1976 (1976). The oxidant must effect the bishydroxylation in the exo form. More particularly, the oxidation can be carried out by means of potassium permanganate or osmium tetroxide or a metal osmate and working in the presence of Nmethylmorpholine oxide or triethylamine oxide or potassium ferricyanide (K3FeCN6).
According to the invention the osmium tetroxide is employed in a catalytic amount which results in a more effective control of osmium residues in the product. The reaction with the osmium may occur with as little as about 0.06 mol% to about 0.1 mol% which takes respectively from about 21 to about 5 hours. The reaction preferably takes place with about 0.06 mol% osmium tetroxide. The oxidation may take place in an aqueous-organic solvent medium such as water-z-butanol or water-acetone, more preferably water-acetone. An ether solvent such as ί-butyl methyl ether or di-z25 propyl ether may additionally be present when the oxidation is carried out in an water-acetone solvent medium. A preferred range of volume amounts of ether:acetone:water solvent mixture is about 1.9:16.7:1 of ether/acetone to about 11.1:7.4:1; more preferred is 11.1:16.7:1 to 16.7:16.7:1.
The bishydroxylation can also be effected in the same manner on a mixture of the diastereomers (I) and (Γ), i.e., without them having to be separated before effecting the bishydroxylation.
The (IR) diastereomer of formula I, wherein R is a group of formula II, can be isolated as salts of optically-active organic acids, and more especially from a mixture of diastereomeric compounds of formulae I and Γ by diastereo-selective crystallization using such optically-active organic acids. One useful optically-active organic acid is L-dimethoxysuccinic acid. The salt formation using L-dimethoxysuccinic acid is carried out in an appropriate organic solvent such as a ketone or an aliphatic alcohol, particularly advantageous is IPA. According to the invention, Ltartaric acid is another useful optically-active organic acid. Tire salt formation using L-tartaric acid acid is carried out in a solvent such as an aqueous-organic solvent mixture wherein the organic
APlPi 99/01486
AP 00942 solvent such as an aliphatic alcohol such as IPA. The use of L-tartaric acid results in improved yields and enantiomeric purity of the desired diastereomer (I).
The dihydroxy moieties of a compound of formula I, wherein R is hydrogen or a group of 5 formula II, can be protected in the form of an ester or acetal/ketal to yield a product of formula VIII
wherein R is hydrogen or a group of formula II, and R’, and R”, are as defined before.
In general, the protection of the hydroxy groups is achieved under either esterification or acetalization/ketalization conditions. For example, esterification takes place by reacting an acyl containing group such as acetic acid or propionic acid in the presence of p-toluenesulfonic acid in an organic solvent such as an aromatic hydrocarbon, for example, benzene or toluene, by separating the water gradually, for example azeotropically, as it is formed. For example, acetalization/ ketalization is effected by reacting an aldehyde or a ketone, possibly in the form of ketal, in the presence of an acid such as TFA in an organic solvent such as an aliphatic alcohol, for example, IPA, aromatic hydrocarbon, for example, benzene or toluene, or ether, for example, ί-butyl methyl ether or di-ipropyl ether, at about 50°C to about the boiling point of the reaction mixture. When an ether solvent is used acetic may acid may also be present which results in formation of a salt of the compound of formula IV that is extractable in water. A preferred ketalization medium, according to the invention, comprises tlie use of 2,2-dimethoxypropane, TFA and IPA to give improved yield and enantiomeric excess of the product. The reaction takes place at about 70°C.
The product of formula VIII wherein R is a group of formula II can be transformed into a product of formula VIII wherein R is hydrogen by hydrogenolysis. In general, the hydrogenolysis is carried out by means of hydrogen, which is optionally pressurized, in the presence of a catalyst such as palladium on charcoal in an organic solvent such as an alcohol, for example, methanol, ethanol or IPA, at about 0°C to about 50°C. A product of formula VIII wherein R is hydrogen is also be formed employing the same hydrogenolysis reagents and conditions on the salt of the compound of formula IV wherein R is a group of formula II.
The product of formula VIII wherein R is hydrogen can be transformed into a product of formula IX
98710/66 /d/dV
AP 00942
wherein R’, and R”, are defined as before and G2 is an amino protecting group, by the selective introduction of an appropriate protecting group.
The protecting groups are selected from those which can later be removed selectively. These protecting groups include the following, which are particularly well suited: /-butoxycarbonyl, chloroacetyl, methoxymethyl, trichloro-2,2,2-ethoxycarbonyl, t-butyl, benzyl, p-nitrobenzyl, pmethoxybenzyl, diphenylmethyl, trialkylsilyl, allyloxycarbonyl, and benzyloxycarbonyl groups, wherein the phenyl ring is optionally substituted by halo, alkyl or alkoxy. Among the protecting groups which are particularly well suited, one can mention those described by T.W. Greene and
P.G.M. Wuis, Protecting Groups in Organic Synthesis, Chapter 7, 2nd edition, John Wiley & Sons (1991). The t-butoxycarbonyl group is of particular interest.
The product of formula IX wherein G2 is t-butoxycarbonyl can be obtained directly from a product of formula VIII wherein R is the group of formula II by simultaneous hydrogenolysis and t-butoxycarbonylation.
For example, the reaction is carried out by simultaneously reacting hydrogen in the presence of a catalyst such as palladium and charcoal and di-t-butyl dicarbonate with a product of formula VIII wherein R is a group of formula II in an organic solvent such as an alcohol, for example, methanol, ethanol or IPA, about 0°C to about 50°C. This reaction is particularly useful where R’, and R”, taken together form an optionally substituted methylene.
Alternatively, the product of formula IX wherein G2 is t-butoxycarbonyl can be obtained in two steps from a product of formula VIII wherein R is the group of formula II by first effecting the hydrogenolytic removal of the group of formula II to yield the corresponding product wherein R is hydrogen, and second effecting the t-butoxycarbonylation of that product. The hydrogenolytic removal is effected as previously described, and the t-butoxycarbonylation is effected in water under alkaline conditions using (Boc)2O.
The product of formula IX is then oxidized into a product of formula X
AP/P/ 9 9 / 0 1 4 86
AP Ο Ο 94 2
wherein R’,, R”, and G, are defined as above.
In general, the oxidation is conducted by means of ruthenium oxide (RuO4), which can be optionally generated in situ from a precursor such as RuO2 or RuCl3 in the presence of an oxidant selected from a periodate such as sodium periodate, a hypohalite such as hypochlorite or sodium hypobromite or a bromate such as sodium bromate or an organic tertiary amine oxide such as Nmethylmorpholine oxide or triethylamine oxide. The reaction takes place in a solvent such as water lv or a homogeneous or heterogeneous aqueous-organic medium, such as a water-EtOAc mixture.
The oxidation can also be conducted using sodium hypochlorite alone or using potassium permanganate or sodium tungstate in the presence of an oxidant such as sodium hypochlorite, hydrogen peroxide or an alkyl hydroperoxide.
The product of formula X can also be obtained by oxidation of a product of formula VIII wherein R is hydrogen under the conditions described above, followed by the protection of the nitrogen atom of the lactam of formula XI
AP/P/ 99/01486 wherein R’, and R”, are defined as above, by a protecting group as defined above.
The products of formula X and XI are particularly useful for the preparation of a carbo 25 sugar of formula XII
FT
NHGi
RO
OR’ (XII) wherein R2 is carboxy, alkoxycarbonyl, N-alkylaminocarbonyl, hydroxymethyl or alkoxymethyl, and 30 R' and R which may be identical or different, are hydrogen, acyl or aroyl, or R’, and R”, taken
AP 00942 together with the carbon atom to which they are attached form an optionally substituted methylene group whose carbon atom is optionally substituted by one or two groups, which may be identical or different, selected from alkyl or phenyl, or two alkyl taken together can form cycloalkyl, and G, is hydrogen or a protecting group G2 for the amino function. More particularly, R2 is an ethylaminocarbonyl group or hydroxymethyl group, and R' and R together form an isopropylidene group.
The product of formula X can be transformed into a product of formula XII under conditions which are appropriate for the nature of the substituent R2 which must be introduced.
The product of formula XII wherein R2 is carboxy can be prepared by reacting a mineral base such as NaOH with the product of formula X, followed by the replacement of the protecting group G2 by hydrogen and optionally groups R’, and R”, by hydrogen.
Tire product of formula XII, wherein R2 is carboxy, can be obtained by the replacement of the protective group G2 of formula X with a hydrogen atom, followed by the action of a mineral base such as sodium carbonate, and optionally replacing tire radicals R’, and R”, by hydrogen.
The product of formula XII wherein R2 is alkoxycarbonyl can be prepared by reacting an alkali metal alkoxide with the product of formula X, followed by the replacement of the protecting group G2 by hydrogen and optionally of the groups R’, and R”, by hydrogen.
The product of formula XII, wherein R2 is alkoxycarbonyl can be obtained by the replacement of the protective group G2 of the product of formula X by hydrogen, followed by the action of an alkali metal alkoxide, and optionally replacing the radicals R’, and R”, by hydrogen.
The product of formula XII wherein R2 is N-alkylaminocarbonyl can be prepared by reacting an alkylamine with the product of formula X, followed by the replacement of the protecting group G2 by hydrogen and optionally of the groups R’, and R”, by hydrogen.
The product of formula XII, wherein R2 is N-alkylaminocarbonyl, can be obtained by the replacement of tire protective group G2 of the product of formula X by hydrogen, followed by the action of an alkylamine, and optionally replacing the radicals R’, and R”, by hydrogen.
The product of formula XII wherein R2 is a hydroxymethyl group can be prepared by reacting a reducing agent such as a borohydride, for example, sodium or potassium borohydride, with the product of formula X, followed by the replacement of the protecting group G2 by hydrogen and optionally of the groups R’, and R”, by hydrogen.
The product of formula XII, wherein R2 is a hydroxymethyl radical, can be obtained by replacement of protective group G2 of the product of formula X by a hydrogen atom, followed by
AP/P/ 9 9/01486
ΑΡο ο 9 4 2 the action of a reducing agent such as sodium or potassium borohydride, and optionally replacing the radicals R’, and R”, by hydrogen.
The present invention also encompasses the isolation of the 5 (IS) diastereomer of the compound of formula Γ using optically-active organic acids of the opposite configuration to those described for isolating the (1R) diastereomer of the compound of formula I. According to the invention, the (IS) diastereomer of the compound of formula Γ can then be converted the corresponding (IS) diastereomers of compounds of formulae IV, VI, VII, VIII, IX and X employing the methods used in preparing the (1R) diastereomeric compounds of those formulae.
The starting materials and intermediates are prepared by the application or adaptation of known methods.
Hie compounds of formulae XIII and ΧΙΙΓ
wherein R, and Ar are as previously defined, can be obtained by a Diels-Alder reaction between a mixture of homochiral amines of formulae
R, R,
Ar-^7^NH2 an(J Ar-4^NH2 wherein R, and Ar are as previously defined, in the form of a salt, preferably with a mineral acid such as HCI, formaldehyde and cyclopentadiene working under the conditions described by S.D. Larsen and P.A. Grieco, J. Amer. Chem. Soc„ Vol. 107, 1768-1769 (1985). This method leads to a mixture of two diastereoisomers. The diasteromers may be separated using L-dibenzoyl tartaric acid as described by C. K.-F. Chiu in Svn. Comm., 26(3), 577 (1996).
The compounds of formulae III and ΙΙΓ wherein R is hydrogen can be obtained by hydrogenolysis of the compounds of formula XIII and ΧΙΙΓ by means two step procedure. First the compounds are treated with 2,2,2-trichlorethoxycarbonyl (Troc) chloride or β(trimethylsilyl)ethoxycarbonyl (Teoc) chloride to give the corresponding Troc or Teoc derivatives (carbamates) and treating with Zn in an alcoholic solvent such as ethanol with heating, or Zn in an organic acid solvent such as acetic acid at room temperature.
AP/P/ 9 9/01486
AP Ο Ο 9 4 2
The present invention is further exemplified but not limited by the following illustrative examples which illustrate the preparation of the compounds according to the invention.
In the nuclear magnetic resonance (NMR) spectra the chemical shifts are expressed in ppm relative to tetramethyl silane. Abbreviations have the following significance: s=singlet; d=doublet; t=triplet; m=multiplet; dd=doublet of doublets; ddd=doublet of doublets of doublets; dt=doublet of triplets, b=broad.
Example la
Preparation of 2-(a-S-Methylbenzyl)-2-azabicyclo[2.2.1]hept-5-ene
In a 2 L reactor is charged 255 g of (S)-(-)-a-methylbenzylamine and 300 mL of water. The suspension is cooled to -5°C and a solution of 185 mL of concentrated HCI in 100 mL of water is added with stirring over one hour. The pH of the mixture is adjusted to between 5 and 6.5. Stirring is continued for 30 minutes and then 242 mL of 37% formaldehyde solution is charged. After stirring for an additional 40 minutes, cyclopentadiene (-270 mL) is distilled directly into the reaction mixture. The resultant mixture is stirred vigorously overnight at -5°C. The completion of the reaction is determined by high-performance liquid chromatography (HPLC). The resulting two layers are separated and the aqueous layer is washed with 250 mL of heptane before basifying to a pH 11 with 168 mL of 50 % NaOH solution and crushed ice. The organic mixture is then extracted with 2 x 500 mL and 2 x 300 mL portions of EtOAc. The combined extracts are washed with 200 mL of cold water, followed by 200 mL of saturated NaCl solution, dried over anhydrous Na2SO4 and filtered. The clear filtrate is concentrated by rotary evaporation to yield 408.4 g (97.4% ) of a yellow oil, 2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]hept-5-ene, in a diastereomeric ratio of 77.1:22.9 in favor of the desired isomer.
Ή NMR (500 MHz, CDC13): δ 1.35 (d, 2H); 1.46 (d, IH); 1.62 (d, IH); 2.89 (d, IH); 3.05 (m, IH); 4.13 (s, IH); 6.11 (d, IH); 6.32 (m, IH); 7.26 (d, 2H); 7.33 (d, 2H); MS (El, 70eV) m/z (relative intensity): 199 (M+, 70)
Example lb
Preparation of 2-(a-S-Methylbenzyl)-2-azabicyclo[2.2.1 ]hept-5-ene
Into a 250-mL three-necked flask equipped with a cooling apparatus and stirring system, a solution is introduced under an argon atmosphere, which solution consists of 20 g of (S)-(-)-amethylbenzylamine (165 mmol) in 60 mL of water whose pH is adjusted to 6.1 by the addition of 17 mL of 36% HCI (W/V). After cooling to 5°C, 20 mL of a 37% (W/V) aqueous formaldehyde solution are added. The solution is stirred for 5 minutes at 5°C; then 21.8 g of cyclopentadiene (330 mmol) are added. The mixture is stirred for 16 hours between -5 and 0°C. The aqueous phase is separated by decanting and then washed with 50 mL of pentane. Neutralization to pH 8 is achieved by addition of concentrated NaOH. Two extractions are then carried out, each with 70 mL of EtOAc. The pH of the aqueous phase is adjusted to 11 by the addition of concentrated NaOH. followed by
8 V tO / 6 6 /d/dV
AP 00942 two extractions, each with 70 mL of EtOAc. The organic phases are combined, and then washed two times with 50 mL of water, and then they are dried over Na2SO4. After filtration and concentration to dryness at a reduced pressure, the yield consists of 33.1 g of 2-(a-S-methyl-benzyl)-2azabicyclo[2.2.1]hept-5-ene in the form of a slightly yellow oil.
Example 2
Preparation of 5R,6S-Dihydroxy-2-(a-S-methylbenzyl)-2-azabicyclo-[2.2.1 Jheptane
Into a 500-mL three-necked flask equipped with a cooling apparatus and a stirring system, containing a solution of 20 g of 2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]hept-5-ene (75.34 mmol) in 220 mL of /-butanol, 12 g of N-methylmorpholine oxide in 32 mL of water, at a temperature of approximately 25°C, are added, then 6.3 mL of a 25% (W/V) solution of osmium tetroxide (OsO4) in /-butanol are added slowly. The stirring is continued for 2 hours at a temperature of approximately 20°C, then for 3 hours at 65°C. After evaporation of the ί-butanol at a reduced pressure, the residue is redissolved in 350 mL of IPA. After concentration to dryness at a reduced pressure, 24 g of cis-5,6-dihydroxy-2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]heptane are produced in the form of an oil. 14 g of 5R,6S-dihydroxy-2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]heptane are produced by crystallization in cyclohexane, with an isomeric purity of more than 95%.
The NMR spectrum, determined in deuterochloroform, shows the following shifts (d): 1.21 (3H, d); 1.38 (1H, d); 1.59 (1H, d); 2.22 (2H, m); 2.45 (1H, dd); 2.95 (1H, s); 3.99 (1H, q); 3.78 (1H, d); 3.90 (1H, d); 7.28 (5H, m).
Example 3 a
Preparation of 5R,6S-Dihydroxy-2-(a-S-methylbenzyl)-2-azabicyclo-[2.2.1]heptane
A solution of 0.5 mmol of a mixture (78/22 in mol) of 5R,6S-dihydroxy-2-(a-Smethylbenzyl)-2-azabicyclo[2.2.1]heptane and 5S,6R-dihydroxy-2-(a-S-methylbenzyl)-2azabicyclo[2.2.1]heptane and 0.5 mmol of L-dimethoxy-succinic acid in 1 mL of IPA is stirred for
24 hours at a temperature ranging from 25°C at the beginning to 5°C. The crystals obtained are separated by filtration and dried. One thus obtains 110 mg of 5R,6S-dihydroxy-2-(a-Smethylbenzyl)-2-azabicyclo[2.2.1]heptane with an enantiomeric excess of 97%.
The mixture of 5R.6S-dihydroxy-2-(a-S-methylbenzyl)-2-azabicycIo-[2.2.1]heptane and
5S,6R-dihydroxy-2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]heptane (78/22 in mol) may be obtained in the following manner;
In a 250 mL three-necked round-bottom flask provided with a coolant and a stirring system, containing a solution of 7 g 2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]hept-5-ene (35 mmol) in
70 mL of /-butanol, is added, at a temperature of approximately 25°C, 4.12 g of Nmethylmorpholine oxide in 11 mL of water, then, 360 mL of a 2.5% solution (p/v) of osmium tetroxide (OsO4) in /-butanol is slowly added. The mixture is stirred for 1 hour at a temperature of
8 7 I n / 6 6 /d/dV
AP 00942 approximately 20°C! and then for 4 hours at 65°C. After the evaporation of the z-butanol under reduced pressure, the residue is taken up in 150 mL of IPA. After concentrating until dry under reduced pressure, one obtains 8.27 g of a product, the NMR spectrum of the proton of which shows that it is composed of a mixture (78/22 in mol) of 5R,6S-dihydroxy-2-(a-S-methyl-benzyl)-25 azabicyclo[2.2.1]heptane and 5S,6R-dihydroxy-2-(a-S-methyl-benzyl)-2-azabicyclo[2.2.1]heptane.
Example 3b
Preparation of 5R,6S-Dihydroxy-2-(a-S-methylbenzyl)-2-azabicyclo-[2.2.1 ]heptane
In a 50 mL one-necked round bottom flask provided with a magnetic stirrer and a coolant is charged 1 g of 2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]hept-5-ene (5 mmol), 2.5 mL of di-ipropyl ether, 2.5 mL of acetone, 0.9 mL of 58 w% aq. N-methylmorpholine oxide and 0.15 mL water. The mixture is stirred for 5 minutes and 9 mg of solid K2Os04»2H20 is charged in once and stirring is continued at room temperature for 25 minutes. The mixture is then stirred at reflux for
7.5 hours. HPLC shows after that time a 95% completion of the oxidation reaction. To the brown mixture cooled to room temperature is added a solution of 630 mg of sodium sulfite in 4 mL of water. The biphasic mixture is stirred at room temperature for 1 hour. Most of the organic solvents are evaporated under reduced pressure, 5 mL of di-z-propyl ether are added. The aqeous phase is separated by decantation and reextracted by 2 x 5 mL di-i-propyl ether. The combined organic phases are washed with an aqueous saturated sodium chloride solution, dried on Na^SO.,, filtered and evaporated under reduced pressure to yield 1.04 g (89%, corrected yield=86%) of 5R,6Sdihydroxy2-(a-S-methylbenzyl)-2-azabicyclo-[2.2.1]heptane as an oil that precipitates on standing. The product is 95% mol. pure by ‘H NMR in CDC13 (contain 4 mol. % N-methylmorpholine + 0.6 mol. % starting material).
Example 3c
Preparation of 5R,6S-Dihydroxy-2-(a-S-methylbenzyl)-2-azabicyclo-[2.2.1]heptane L-tartrate
To a 2 L reactor is charged 210 g of 2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]hept-5-ene,
1,200 mL of 2-methyl-2-propane and 182 mL of 4-methymorpholine N-oxide. To this mixture is charged, in dropwise fashion, 8 mL of a 2.5 % solution of osmium tetroxide in ζ-butanol. Under nitrogen the mixture is heated to 62°C with vigorous stirring for 22 hours. Tire reaction mixture is concentrated by rotary evaporation at 60°C. 300 mL of IPA is charged and the solution is again concentrated at 60°C to yield 246 g of 5R,6S-Dihydroxy-2-(a-S-methylbenzyl)-2-azabicyclo35 [2.2.1]heptane as a dark brown syrup. The crude product is suspended in 1.8 L of 75% IPA at 40°C. To the suspension is added 158.2 g of L-tartaric acid with vigorous stirring. Agitation is continued at 40°C for 2.5 hours. The mixture is cooled to 30°C, filtered, washed with 500 mL of 75% IPA and 200 mL of IPA, then dried at 70°C in vacuo for 16 hours to give 269.5 g of the desired L-tartrate salt as a cream colored solid (MP 143-145°C, diastereomeric ratio of 94.2:5.8 ).
*H NMR (500 MHz, CDC13): δ 1.3 (d, 3H); 2.5 (m, 2H); 4.18 (s, 2H); 7.36 (t, 2H); 7.4 (t, 2H); MS (El, 70eV) m/z (relative intensity): 233 (M+, 13)
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AP 00942
Example 4a
Preparation of 5R,6S-isopropylidenedioxy-2-(a-S-methylbenzyl)-2-azabicyclo-[2.2.1 [heptane
Into 500-mL three-necked flask, equipped with a cooling apparatus and a stirring system, containing a solution of 18.4 g of 5R,6S-dihydroxy-2-(a-S-methylbenzyl)-2azabicyclo[2.2.1 [heptane (76 mmol) in 130 mL of toluene, 31.7 g of 2,2-dimethoxypropane (304 mmol) and then 13 g of TFA are added slowly (114 mmol). The mixture is heated for 4 hours 10 minutes at 65°C. After cooling to 30°C and concentration in the rotary evaporator to eliminate the toluene, the excess 2.2-dimethoxypropane and partially the TFA, the reaction mixture is taken up in dichloromethane, then it is neutralized by the addition of 100 mL of 2 N NaOH. After decanting, drying of the organic phase over Na2SO4, filtration, treatment with decolorizing charcoal (30 g) for 30 minutes at the boiling point of dichloromethane, filtration through Clarcel® and concentration to dryness at reduced pressure, the yield consists of 18.8 g of 5R,6S-isopropylidenedioxy-2-(a-Slo methylbenzyl)-2-azabicyclo-[2.2.1 [heptane. whose structure is confirmed by the proton NMR spectrum, which, determined in deuterochloroform, shows the following shifts (d): 1.22 (3H, d); 1.23 (6H, s); 1.31 (IH, d); 1.57 (IH, d); 2.08 (IH, d); 2.34 (IH, broad s); 2.45 (IH, dd); 3.06 (IH, s);
3.40 (IH, q); 4.09 (IH, d); 4.19 (IH, d); 7.26 (5H, m).
Example 4b
Preparation of 5R,6S-isopropylidenedioxy-2-(a-S-methylbenzyl)-2-azabicyclo-[2.2.1]heptane
To a 2-liter, 4-neck, jacketed cylindrical reactor equipped with a thermocouple, overhead stirrer and condenser is charged 223 g of 5R,6S-dihydroxy-2-(a-S-methylbenzyl)-225 azabicyclo[2.2.1]heptane L-tartrate followed by 1200 mL 1PA. Agitation is begun and the flask charged 286 mL of 2,2-dimethoxypropane and 44.6 mL of TFA. The suspension is heated to 72°C until all the solids dissolved. After 5 hours, the reaction is cooled to 65°C and the contents transferred to a 3 L round bottom flask. Approximately 1100 mL of solvent is removed at 48°C and 124 mbar vacuum. To the original 2-liter, 4-neck, jacketed cylindrical reactor is added 1.2 L of 2 M
NaOH with stirring at 25°C. To the NaOH solution, is charged the residue from the distillation described above (~ 700 mL of solution). The tan solution is cooled to 25°C over 40 minutes. Solids begin to precipitate from the solution at 28°C. The suspension is stirred several hours before being filtered through an 11 cm Buchner funnel fitted with Whatman # 1 filter paper. The filter cake is washed with 300 mL of water. The off-white solids are slurried in water for 13 hours and refiltered, washed with water and air dried. The solids are then vacuum dried at 50°C to yield 112 g of 5R.6Sisopropylidenedioxy-2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]heptane as a white solid which according to HPLC is diastereomerically pure.
Ή NMR (500 MHz, CDC13): δ 1.28 (s. 3H); 1.27 (d, 3H); 1.39 (s, 3H); 1.63 (d, IH); 2.27 (d. IH);
2.4 (d, IH); 2.51 (dd, IH); 3.12 (s, IH); 3.46 (q, IH); 4.2 (dd, 2H); 7.28 (m, 5H); MS (El, 70eV) m/z (relative intensity): 273 (M+, 8.4)
98^10/66 /d/dV
Example 5 a
AP 0 0 9 4 2
Preparation of 5R,6S-isopropylidenedioxy-2-(/-butoxycarbonyl)-2-azabicyclo-[2.2.1 ]heptane
In a 250-mL three-necked flask equipped with a stirring system are combined, 0.5 g of 5% palladium on charcoal, 5 g of 5R,6S-isopropylidene-dioxy-2-(a-S-methylbenzyl)-2azabicyclo[2.2.1]heptane, 3.98 g of di-i-butyl dicarbonate and 36 mL of methanol. The apparatus is purged with argon and then with hydrogen, and then it is placed under a hydrogen atmosphere at 25 °C. The reaction is continued for 5 hours by carrying out a purge with hydrogen every 15 minutes to eliminate the carbon dioxide formed.
After filtration through Clarcel1® and concentration to dryness at a reduced pressure, the yield consists of 4.84 g of 5R,6S-isopropylidenedioxy-2-(/-butoxycarbonyI)-2-azabicyclo[2.2.1]heptane, whose structure is confirmed by the NMR spectrum, which, determined in dimethyl sulfoxide-d6, shows the following chemical shifts (d): 1.16 (s, 3H); 1.28 (s. 3H); 1.32 (s, 1H); 1.34 (s, 3H); 1.65 (d, 1H); 2.38 (m, 1H); 2.65 (d, 1H); 2.99 (m, 1H); 3.84 (m, 1H); 3.94 (d, 1H); 4.16 (d, 1H).
Example 5b
Preparation of 5R,6S-isopropylidenedioxy-2-(/-butoxycarbonyl)-2-azabicyclo-[2.2.1 ]heptane
To a 2-liter, 4-neck, jacketed, cylindrical reactor equipped with a thermocouple, overhead stirrer, gas bladder and a septum for nitrogen and hydrogen inlet is charged in succession; 140 g of 5R,6S-isopropylidenedioxy-2-(a-S-methylbenzyl)-2-azabicyclo[2.2.1]heptane, 13.4 g of 10% Pd/C and 900 mL of methanol. The stirred suspension is sparged with nitrogen for 10 minutes followed by hydrogen for 10 minutes at 25°C. This procedure is repeated every 30 minutes and the reaction is monitored by TLC (silica gel, EtOAc, visualized with iodine). After 3 hours, the reaction is 50% complete according to TLC. To this partially reduced solution is charged 56 g of diί-butyldicarboxylate over 10 minutes followed by a nitrogen/hydrogen sparge as described above. Every 30 minutes, an additional 10 g of di-t-butyldicarboxylate is added, followed by a nitrogen/hydrogen sparge until a total of 112 g of di-t-butyldicarboxylate is added (56 g plus 10 g charges). The reaction mixture is stirred overnight at 25 °C. The Pd/C suspension is filtered through a 9 cm Buchner funnel fitted with # 54 filter paper and a bed of 5 g of Celite and the reactor and filter cake are washed with 100 mL of methanol. The filtrate is placed in 2 liter 1-neck round bottom flask and 750 mL of solvent is removed at 40°C and 105 mbar (~ 250 mL of a light yellow solution remained). To the original reaction vessel, is charged 1 L of water which is cooled to 10°C. The yellow residue from the above distillation is added to the cold water in virtually one portion. 5R,6SIsopropylidenedioxy-2-(r-butoxycarbonyl)-2-azabicyclo[2.2.1]heptane precipitates from the solution as a white solid. The slurry is stirred for 30 minutes at 6°C before filtering and washing with water. The resulting white solids are vacuum dried at 60°C to yield 129.6 g of white solid which according to chiral HPLC is enantiomericaliy pure.
Ή NMR (500 MHz. CDClj): δ 1.28 (s, 3H); 1.4 (s, 3H); 1.45 (s, 9H); 1.87 (d, 1H); 2.53 (s, 1H); 2.82 (d, 1H); 3.17 (dd, 1H); 4.09 (m, 2H); 4.2 (m, 2H); MS (FAB-LRP) m/z (relative intensity); 270 ((M + H)+, 9.4)
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Example 6a
Preparation of 5R,6S-isopropylidenedioxy-2-(i-butoxycarbonyl)-2-azabicyclo-[2.2.1]heptan-3-one
In a 30-mL tube, 270 mg of 5R,6S-isopropylidenedioxy-2-(z-butoxycarbonyl)-2azabicyclo[2.2.1]heptane (1 mmol) and 40 mg of RuO2-H2O (0.3 Eq) are introduced. 10 mL of EtOAc and 720 mg of water (40 Eq) are added. Then, 2.14 g of sodium periodate (10 Eq) are added, and the tube is sealed hermetically. The stirring is continued for 16 hours at 50°C. The reaction mixture is filtered through Clarcel®, and then two extractions are carried out, each with 20 mL of EtOAc. The organic phases are dried over Na2SO4. After the filtration and concentration to dryness at a reduced pressure, 245 mg of a solid are obtained, containing 68% of 5R,6Sisopropylidenedioxy-2-(t-butoxycarbonyl)-2-azabicyclo[2.2.1]heptan-3-one and 32% of starting material. The structure of the product obtained is confirmed by the NMR spectrum, which, determined in dimethyl sulfoxide d6, shows the following chemical shifts (d): 1.38 (9H. s); 1.23 (3H,
s); 1.33 (3H, s); 1.85 (1H, d); 1.93 (1H, d); 2.69 (1H, s); 4.24 (1H, s); 4.41 (1H, d); 4.51 (1H, d).
Example 6b
Preparation of 5R,6S-isopropylidenedioxy-2-(/-butoxycarbonyl)-2-azabicyclo-[2.2.1]heptan-3-one
To a 2 liter 4-neck jacketed cylindrical reactor equipped with a thermocouple, overhead stirrer, and condenser is charged in succession: 120 g of 5R,6S-isopropylidenedioxy-2(/-butoxycarbonyl)-2-azabicyclo-[2.2.1]heptane, 0.3 g of RuO2, 201.2 g of sodium bromate, 960 mL of EtOAc, and 1000 mL of water with stirring. The reaction mixture is heated to 45°C and stirred at this temperature for 15 hours. The stirring is discontinued and the aqueous layer discarded.
Saturated NaCl (500 mL) is added to the reaction vessel and the suspension is stirred for 10 minutes. Stirring is again discontinued, the layers are allowed to separate and the aqueous layer removed. A 33% maleic acid disodium salt solution (500 mL) is charged to the reaction vessel, the suspension stirred for 5 minutes and the layers again separated. The organic layer is then filtered through a bed of Celite to remove the catalyst and the solvent is removed in vacuo. The resulting solid is dried in a vacuum oven to yield 117 g of 5R,6S-isopropylidenedioxy-2-(/-butoxy-carbonyl)-2azabicyclo[2.2.1]heptan-3-one as a white solid contaminated with 5% starting material. A 115 g sample of this material is dissolved in 350 mL of heptane at 85°C and allowed to cool to 25°C over about 3 hours then to 5°C before filtration and drying in vacuo at 60°C. 92 g (74%) of 5R.6Sisopro-pylidenedioxy-2-(/-butoxycarbonyl)-2-azabicyclo-[2.2.1]heptan-3-one is obtained as a white crystalline solid.
Ή NMR: δ 1.32 (m, 3H); 1.48 (m, 12H); 1.82 (m, 1H); 2.1 (m. 1H); 4.43 (m, 1H); 4.48 (m, 1H);
4.6 (m, 1H); MS (FAB-LRP in nitrobenzyl alcohol): 284 ((M + H)+, 10%)
Example 7a
Preparation of 2R,3S-isopropylidenedioxy-4R-amino-lS-ethylaminocarbonyl-cyclopentane benzoate
AP/P/' 9 9/01486
AP 00942
In a Berghoff tube is placed 568 mg of 5R,6S-isopropylidenedioxy-2-(f-butoxycarbonyl)-2azabicyclo[2.2.1]heptan-3-one and 10 mL of a 70% aqueous solution of ethylantine (by weight).
The mixture is heated for 4 hours at 60°C while stining. After cooling, the excess ethylamine and water is eliminated under reduced pressure. After drying under reduced pressure, one thus obtains
98% yield of 650 mg of 2R.3S-isopropylidenedioxy-4-R-z-butoxy-carbonylamino-l-Sethylaminocarbonyl cyclopentane, the structure of which is confirmed by the NMR spectrum of the proton, and the rotatory power of which is [a]D20 = 15.0 (c = 1; methanol).
To a solution of 200 mg of 2R,3S-isopropylidenedioxy-4R-z-butoxycarbonylamino-l-S10 ethylaminocarbonyl cyclopentane in 1.6 mL of anhydrous dichloromethane is added 275 mL of
TFA. The mixture is stirred overnight at a temperature of approximately -5°C. The reaction mixture is poured into 4 mL of 2.5 N aqueous sodium carbonate. The organic layer is concentrated under reduced pressure at a temperature below 25°C. One thus obtains 125 mg of a product which is dissolved in 0.5 mL tetrahydrofuran. To this solution is added 70 mg of benzoic acid. After cooling the solution obtained to a temperature of approximately 0°C, the crystals obtained are separated by filtration and washed in pentane. One thus obtains 138 mg of 2R,3S-isopropylidenedioxy-4Ramino-lS-ethylaminocarbonyl cyclopentane benzoate.
Example 7b
Preparation of 2R,3S-isopropylidenedioxy-4R-amino-lS-ethylaminocarbonyl-cyclopentane trifluoroacetate
In a 25-mL autoclave, equipped with a magnetic stirrer, 1.47 g of 5R,6Sisopropylidenedioxy-2-(i-butoxycarbonyI)-2-azabicyclo[2.2.1]heptan-3-one in a solution in 10 mL of anhydrous toluene is introduced, followed by approximately 0.7 mL of ethylamine. The autoclave is closed and then heated at a temperature between 90 and 100°C for 21 hours. After cooling, the toluene is evaporated, and the dissolution is carried out with 10 mL of dichloromethane and 10 mL of water. After decanting, the organic phase is washed with 10 mL of water. Tire combined aqueous layers are washed in 10 mL of dichloromethane. The combined organic phases are washed with 10 mL of a saturated sodium chloride solution and then dried over Na2SO4. After filtration and concentration to dryness at a reduced pressure, the yield consists of 1.58 g of a product containing 95% 2R,3S-isopropylidene-dioxy-4R-z-butoxycarbonylamino-l-Sethylaminocarbonylcyclopentane, whose structure is confirmed by the NMR spectrum, which, determined in dimethyl sulfoxide-d6, shows the following chemical shifts: 0.95 (t, 3H); 1.14 (s, 3H);
1.31 (s, 12H); 1.55 (m, 1H); 2.11 (m, 1H); 2.64 (m. 1H); 3.00 (q, 2H); 3.77 (m, 1H); 4.23 (m, 1H);
4.54 (m, 1H); 7.07 (d, 1H); 8.12 (t, 1H).
In a 25-mL flask, 1.22 g of 2R,3S-isopropylidenedioxy-4R-t-butoxy-carbonylamino-lSethylaminocarbonylcyclopentane and 10 mL of dichloro-methane are introduced. At a temperature of approximately 25°C, 0.85 g of TFA is added with stirring. After 6 hours of stirring and concentration to dryness, the yield consists of 1.16 g of 2R,3S-isopropylidenedioxy-4R-amino-lSethylaminocarbonylcyclopentane trifluoroacetate, whose structure is.confirmed by the NMR
AP/P/ 9 9/01486
AP 009 4 2 spectrum, which, determined in dimethyl sulfoxide-d6, shows the following chemical shifts: 0.79 (t, 3H); 1.03 (s, 3H); 1.19 (s, 3H); 1.42 (m, 1H); 2.05 (m, 1H); 2.52 (m, 1H); 2.89 (q, 2H); 3.04 (m, 1H); 4.16 (m, 1H).
Example 7c
Preparation of 2R,3S-isopropylidenedioxy-4R-amino-lS-ethylaniinocarbonyI cyclopentane
To a solution of 167 mg of 5R,6S-isopropylidenedioxy-2-(/-butoxycarbonyl)-2azabicyclo[2.2.1Jheptan-3-one in 1 mL of dichloromethane, cooled to 0°C, is added 90 pL of TFA.
The temperature is allowed to rise to 23°C over 40 minutes, then stirred for 22 hours at this temperature. Another 90 pL of TFA is added and then stirred for another one hour at a temperature of 23°C. After evaporating under reduced pressure, one obtains 123 mg of 5R.6Sisopropylidenedioxy-2-azabicyclo[2.2.1]heptan-3-one, the purity of which, determined by HPLC, is approximately 92%, and the structure of which is confirmed by the NMR spectrum of the proton.
15
A solution of 10 g of 5R,6S-isopropylidenedioxy-2-azabicyclo-[2.2.I]heptan-3-one in 100 mL in a 70% aqueous solution of ethylamine (by weight) is heated to 110°C for 20 hours under standard pressure. After cooling, the excess ethylamine is eliminated under reduced pressure, then washed with dichloromethane to eliminate the starting product that did not react. The aqueous layer is then concentrated and dried. One thus obtains 10.54 g of 2R,3S-isopropyIidenedioxy-4R-amino1 S-ethylaminocarbonyl cyclopentane.
The present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof.

Claims (10)

  1. WE CLAIM:
    I. A method for the preparation of a 2-azadihydroxybicyclo[2.2.1]heptane compound of formula:
    wherein * represents an R chirality, *’ represents an S chirality, R is hydrogen or, respectively, a group of formula Ri R1
    Ar (Π) or ‘Ar (II’) wherein R, is alkyl and Ar is optionally substituted aryl, comprising bishydroxylating a bicyclo[2.2.1]heptene compound of formula
    AP/P/ 9 9 / u 1 4 8 6 in the presence of about 0.1 mol % to about 5 mol % of a metal osmate compound or about 0.06 mol % to about 0.07 mol % osmium tetroxide, and an oxidizing agent capable of regenerating
    20 osmium tetroxide.
  2. 2. The method according to claim 1 wherein R is the group of formula
  3. 3. The method according to claim 2 wherein R, is methyl or ethyl, and Ar is optionally substituted phenyl, which when substituted is substituted by one or more methyl or methoxy.
  4. 4.
    The method according to claim 3 wherein R, is methyl, and Ar is phenyl.
    AF 00942
  5. 5. The method according to claim 1 wherein the bishydroxylation is effected using osmium tetroxide at about 0.06 mol % to about 0.07 mol %.
  6. 6. The method according to claim 5 wherein the osmium tetroxide is present at about 0.06 mol
    5 %.
  7. 7. The method according to claim 1 wherein the bishydroxylation is effected using metal osmate at about 0.1 mol % to about 5 mol %.
    10
  8. 8. The method according to claim 7 wherein the metal osmate is present at about 0.2 to about
    0.5 mol %.
  9. 9. The method according to claim 1 wherein the metal osmate is K2OsO4*2H2O.
    15 10. The method according to claim 1 wherein the oxidizing agent capable of regenerating osmium tetroxide is N-methylmorpholine oxide.
    11. A method for the preparation of an L-tartaric acid salt of the (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound according to claim 1,
    20 comprising treating the (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound according to claim 1 wherein R is a group of formula
    Rt
    AP/P/ 99/01486 with L-tartaric acid.
    12. The method according to claim 11 wherein R, is methyl and Ar is phenyl.
    13. The method according to claim 11 further comprising preparing the
    L-tartaric acid salt of the (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]-heptane compound 30 in a substantially enantiomerically purified state in the presence of a (IS) diastereomer of the 2azadihydroxybicyclo[2.2.1]heptane compound.
    14. The method according to claim 11 wherein the treating occurs in an aqueous-organic solvent mixture.
    35
    15. The method according to claim 14 wherein organic solvent is isopropanol.
    16. The method according to claim 14 wherein the treating occurs in an aqueous-isopropanol solvent mixture having a volume ratio of about 30:70 to about 15:85.
    AP 00942
    17. The method according to claim 16 wherein the volume ratio is about 25:75.
    18. The compound of formula
    HO —R · L-tartaric acid salt wherein R is as defined in claim 11.
  10. 10 19. A method for the preparation of a compound of formula wherein R is as defined in claim 1, R3. and R3,. are hydrogen, alkyl or phenyl, or R3. and R3.. taken 15 together with the carbon atom to which they are attached form cycloalkyl, comprising acid facilitated acetalizing or ketalizing of a compound of formula
    9 8 * I 0 / 6 6 /d/dV
    20 wherein R4. and R4.. are alkoxy, or taken together with the carbon atom to which they are attached form carbonyl, with the (IR) diastereomer of the 2-azadihydroxybicyclo[2.2.1]heptane compound according to claim 1 or salt thereof in isopropanol.
    20. The method according to claim 19 wherein R„. and R4.. are methoxy and R3. and R3.. are
    25 methyl.
    21. The method according to claim 19 wherein acid facilitation is effected using trifluoroacetic acid.
    30 22. The method according to claim 19 wherein R is a group of formula
    AP 00942
    23. The method according to claim 22 wherein R, is methyl and Ar is phenyl.
    5 24. The method according claim 19 wherein the (IR) diastereomer of the 2azadihydroxybicyclo[2.2.1]heptane compound is in the form of an L-tartaric acid salt thereof.
    25. The method for the preparation of a lactam compound of formula wherein R’, and R”, independently are acyl or aroyl, or taken together form an optionally substituted methylene, and G, is hydrogen or an amino protecting group, comprising oxidizing a bis O-protected lR-2-azadihydroxybicyc!o-[2.2.1]heptane compound of formula
    9 8 7 10/66 /d/dV with about 0.1 mol % to about 1 mol % of RuO3 or hydrate thereof in the presence of about 3 equivalents of an oxidant to form the lactam compound with an enantiomeric excess of greater than
    20 or equal to about 95%.
    26. The method according to claim 25 wherein RuO2 is present at about 0.5 mol %.
    27. The method according to claim 25 wherein the lactam compound is formed with an 25 enantiomeric excess of greater than or equal to about 99%.
APAP/P/1999/001486A 1996-10-16 1997-10-15 Process for preparing 2- azadihydroxybicyclo[2.2.1]heptane compounds and the L - tartaric acid salt of the compound. AP942A (en)

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US08/732,024 US5684159A (en) 1995-05-30 1996-10-16 L-tartaric acid salt of a (1R) diastereomer of a 2-azadihydroxybicyclo 2.2.1!heptane compound and the preparation of 2-azabicyclo 2.2.1!heptane compounds
PCT/US1997/018365 WO1998016510A1 (en) 1996-10-16 1997-10-15 Process for preparing 2-azadihydroxybicyclo[2.2.1]heptane compounds and the l-tartaric acid salt of the compound

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ES2216252T3 (en) * 1997-12-25 2004-10-16 Kuraray Co., Ltd. PREPARATION PROCEDURE AND METHOD OF CRYSTALLIZATION OF 2-AZABICICLO (2.2.1) HEPTA-5-EN-3-ONA.
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