AP892A - Niprisan-phytodrug for treatment and management of sickle cell anaemia and methods of preparing and using same. - Google Patents
Niprisan-phytodrug for treatment and management of sickle cell anaemia and methods of preparing and using same. Download PDFInfo
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- AP892A AP892A APAP/P/1998/001207A AP9801207A AP892A AP 892 A AP892 A AP 892A AP 9801207 A AP9801207 A AP 9801207A AP 892 A AP892 A AP 892A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/67—Piperaceae (Pepper family), e.g. Jamaican pepper or kava
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
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Abstract
A phytochemical composition for treating sickle cell disease is provided. The composition is a cold water extraction product of a mixture containing piper guineenses seeds, Pterocarpus osum stem, Fugenia carvophyllum fruit, Sorghum bicolor leaves and potash. Also described are mixtures of phytomaterials used for preparing the extraction product, methods for making the extraction product, and methods for using the extraction product
Description
vfLE OF THE INVENTION
PHYTODRUG FOR TREATMENT AND MANAGEMENT OF SICKLE CELL ANEMIA AND METHODS OF PREPARING AND USING SAME
BACKGROUND OF THE INVENTION
Field of the Invention
The present invention relates to the field of phy todrucjs , and in particular the invention relates to phytodrugs for treatment and management of sickle cell disease and methods of preparing and using same.
S^rior Activities and._Problems in the Field
Sickle Cell Disease (SCD) is a genetic disorder which, in particular, shows its clinical manifestations in the Black race.
At the present time there is no known safe drug for the management of this disease anywhere in the world. Nigeria, being the most populous Black nation in the world, has the highest incidence of £CS>- Conservatively it is estimated that over 2 million Nigerians suffer from SCD while another 25 million Nigerians are carriers.
100,000 babies are born every year with SCD and it causes --, /
Λ.’-X hbproximately 8% of all infant deaths each year.
The morbidity and mortality factors associated with sickle cell disease are well-known. The acute and chronic trauma of the painful crisis are beyond description. In view oi these realities, there is a constant need for drugs which might alleviate the effects of this terrible disease. And the search for such drugs is of the highest priority.
AP/P/ 9 8 / 0 1 2 0 7
ΑΡ£ίΟβ92
The first known case of SCD involving a 20 year old West Indian student studying in America was described by Herrick in 1910. However, centuries before Herrick documented this case, SCD was known to the people of West Africa. In fact, archaeological research in Nigeria has unearthed 700-year-old human bones showing
Γ evidence of sickle cell, infarcts.
SCD is a hereditary disease of the red blood cells ^characterized by the presence of an abnormal gene that causes production of abnormal hemoglobin of the sickle or ”S variety. It is not known exactly when, how, or where the mutation producing the sickle cell gene occurs, but the sickle cell gene is found to be prevalent in malaria endemic regions of tropical Africa.
The relationship of the sickle cell gene to P. falciparum malaria has become a classic example of natural selection in man. The high mortality of children in endemic countries due to malaria makes it a powerful selective influence for factors that confer ,/ resistance. It is generally accepted that the hemoglobin AS system '-'represents a balanced polymorphism in nature and that the force sustaining the high frequency of HbS is endemic malaria, especially
Ej_falciparum malaria. It is thought that human populations in such high malaria endemic areas evolved high frequency of genes that in the heterozygotes hardly causes genetic disease per se and leaves a selective advantage to the population:; concerned, i.e., that the heterozygote sickle state (AS) confers a survival o
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U ix ζ//- ΑΡ Ο Ο Ο 8 9 2
Attorney Doc) et: 1300 .'1001/JHM .^antage over the. normal AA individual. It is postulated that the S gene accords a relative protective effect against severe forms of P. falciparum malaria such that a child with the sickle cell trait (AS) has a better chance of survival to adult age than the
J ' non-sickler. However, the exact mechanism(s) through which HbS 1 ’ protects against severe malaria still remains unclear. /
Accelerated sickling and preferential destruction of ^parasitized red blood cells as compared to non-parasitized red blood cells, especially in a hypoxic micro-environment, has been postulated. Also, the HbS polymer may inhibit parasite growth or interfere with some other critical functions of the parasite. It must be emphasized that these postulates do not suggest that sickle cell trait individuals are resistant to infection by P. falciparum, but rather that they are less likely to di'e of the infection than Individuals with normal Hb. On the other hand, in the sickle cell patient, acute malaria constitutes a life threatening event by .1 means of its propensity for initiating vaso-occulisive and anaemic crises. />.
The acute complications that have constituted major causes of mortality in SCD include the following:
(a) Overwhelming bacterial infection (meningitis and septicaemia from Strep. pneumonia or H. influenzae infection), and in this regard, the combination of penicillin prophylaxis and multi-valent pneumococcal
AP0.0 0 8 9 2
Attorney Docket: 1300.1001/JHM vaccination have been shown to decrease incidence and mortality in the United States and is currently being introduced to some West African Countries;
(b) Acute chest syndrome;
(c) Acute splenic sequestration;
(d) Cerebro-vascular accident (C.VA); and . · (e) Aplastic crisis.
However, painful episodes are by far the most common complications of SCD. About 70% of patients with painful episodes do not even seek professional medical assistance. But one of the primary goals of management of painful episodes is to alleviate pain and suffering. And since painful episodes are never life threatening, it is important also to reduce the level of anxiety in the patient and family. Placebos have no place in the management of pain.
SUMMARY OF THE INVENTION
It is'a primary objective of the present invention to'provide v.7 a phytodrug that is useful in the care and management of sickle { -J
-cell disease m persons afflicted with such disease. It is also an objective of the invention to provide methods for preparing a useful phytodrug and for treating persons afflicted with sickle cell disease.
With the foregoing in mind, the present invention provides a composition for treating sickle cell disease comprising a cold
AP/P/ 98/01207
extraction product of a mixture containing Piper qd'ineenses
Pterocarpus osum stem, Eugenia_saryjaphylllUB fruit, and im bicolor (caudatum) leaves. The mixture may also include h. In a preferred form of the invention the mixture may de from about 12 to about 17 parts by weight of said Piper, enses seeds, from about 15 to about 19 parts by weight/of said carpus osum stem, from about 12 to about 18 parts by weight of Eugenia caryophyllum fruit, from about 25 to about 32 parts by said Sorghum bicolor leaves and from about 15 to about 22 s by weight of said potash. In its presently most preferred , the mixture may include about 15.8 percent by weight of said >r guineenses seeds, about 18.4 percent by weight of said -ocarpus osum stem, about 13.2 percent by weight of said Eugenia yophyllum fruit, about 31.6 percent by weight of said Sorghum olor leaves, and about 21.0 percent by weight of said potash. The invention also provides a composition for preparing a drug r^ctive for treating sickle cell disease which comprises a ctu-T-e containing Piper guineenses seeds, Pterocarpus osum stem, genia caryophyllum fruit, and Sorghum bicolor leaves. The xture may also include potash.
In another aspect the invention provides a method for eparing a composition for treating sickle cell disease. The -thod includes the steps of forming a mixture containing Piper ll,nee.ns.es. seeds, Pterocarpus osum stem, Eugenia caryophyllum ^0210/86 /d/dV
Γ“·
000099 fruit, and Sorghum bicolor leaves and subjecting said mixture to extraction using cold water to thereby form an extract containing a drug material effective for treating said sickle cell disease.
The mixture may also include potash.
The invention also provides a method for treating a human • afflicted with sickle cell disease. In this aspect /of the invention the method includes forming a mixture containing Piper guineenses seeds, Pterocarpus osum stem, Eugenia carvophyllum fruit, and Sorghum bicolor leaves, subjecting said mixture to extraction using cold water to thereby form an extract containing a drug material effective for treating said sickle cell disease, c
and treating a human afflicted with sickle cel] disease with said drug material. In this aspect of the invention also the mixture may include potash. In accordance with the invention, a dose of approximately 166.7 mg of said drug material may be administered orally to a person afflicted with sickle cell disease.
(*
DET7KILED DESCRIPTION OF PREFERRED EMBODIMENTS OF THE INVENTION
In accordance with the invention, a composition for treating sickle cell disease is provided. The composition consists of a cold water extraction product of a mixture containing Piper guineenses seeds, Pterocarpus osum stem, Eugenia carvophyllum fruit, and Sorghum bicolor leaves. Preferably the initial mixture may also include potash. Generally the initial mixture from which the extraction product is prepared will include from, about 12 to
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AP 0 e is β 9 2 parts by weight of Piper ciuineenses seeds, from about 15 to about 19 parts by weight of Pterocarpus osum stem, from about 12 to about 18 parts by weight of Eugenia oarvophyHum fruit, from . about 25 to about 32 parts by weight of Sorghum bicolor leaves, and from about 15 to' about 22 parts by weight of said potash.
, Phytochemical screening indicates that the extraction product contains flavonoids, alkaloids1, saponins, tannins, glycosides and traces of anthraquinones. However, the extraction product does not ^^include steroids or fatty acids. In this regard it is postulated that· the major contributor to the anti-sickling properties of, the drug materials of the invention may be the tannin content of the Eugenia carvophyllum fruit. Moreover, it is believed also that the other ingredients of the extracted drug materials may have a synergistic effect which enhances the beneficial effect of the drug materials overall when used in connection with the treatment and uanagement of sickle cell disease.
In accordance with the .··/ /«^initial mixture from which includes about 15.8 percent about 18.4 percent by weight percent by weight of Eugenia caryophvllum fruit, about 31.6 percent by weight of Sorghum bicolor leaves, and about 21.0 percent by weight of potash.
preferred form of the invention, the the extraction product is prepared by weight of Piper guineenses seeds, of Pterocarpus osum stem, about 13.2
AP/P/ 9 8/01207
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The various raw materials described above are weighed, mixed together and then subjected to cold water extraction for 24 hrs.
The fluid containing the extract is then decanted and filtered and the residue is discarded.; The filtrate is freeze-dried for 48 . hours using a Finn - Aqua Lyovac GT3 freeze dryer to provide a dry . . . . / powder containing the active drug materials extracted from the initial mixture.
The powder containing the active drug materials may then be formulated into capsule dosage form. Each capsule may preferably contain 166.7 mg of the freeze-dried powder and inert additives in sufficient quantities to provide a 300 mg per capsule.
It has been determined that the most effective daily dosage consists of four capsules for adults and two capsules for children. The daily dosages should be administered in two divided doses with one-half of the daily dosage taken twice daily. Preferably the .separate divided doses will be taken in the morning and again in the evening.
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In accordance with the invention, it was initially observed that the extracted drug materials described above protected red blood cells obtained from sickle cell patients, against sickling when such cells were exposed to low oxygen tension. This effect lasted for over 48 hours while the protection was about 91%. Furthermore, laboratory data clearly indicated the dose dependant reversal of already sickled red blood cells using the described
ΑΡϋ 0 0 09 2 t extracts. This reversal also lasted for over 48 hour's and effect was 100%. These tests indicated that the extract had ntific justification for possible beneficial effect in sickle patients. Safety evaluation tests were therefore conducted.
t ί data obtained from such evaluation tests clearly show that the iuct is very safe. Carcinogenicity and mutagenicity evaluation also conducted and the obtained results indicate that the aribed drug materials are devoid of such adverse effects. t>jhe World Health Organization (WHO) has policies concerning development of herbal medicine. WHO clearly advises that· when lant based product (e.g. herbal medicine) is safe and indicates ioacy, it can be subsequently standardized and formulated into table dosage form for clinical trials. In accordance with the .sent invention, the procedures and policies recommended by WHO e been followed in developing and testing the efficacy of the :g .materials of the present invention. Thus, the drug materials -the invention were standardized and formu1ated into capsule sa/qq form as described above. /..
Clinical trial phase I was conducted using staff of the gerian National Institute for Pharmaceutical Research and /elopment (N1PRD) as volunteers. The clinical parameters of the ood chemistry, the liver functional status as well as kidney notional status were assessed. It was thus determined that the
API?! 9 8 / 0 1 2 0 7
000892 drug materials of the invention had no adverse effect on the volunteers -regarding all the parameters studied.
Initially during the phase II clinical trials, the staff of NIPRD went regularly to the homes of patients so as to make vital observations with respect to the response of the patients to the • new drug materials of the invention. Later, due to the encouraging results obtained, the visits were reduced to once a month. After one and one-half years, the patients were seen only about once every two months at clinics established by NIPRD. The clinical status of each patient was assessed by medical doctors at?these clinics.
Laboratory tests were conducted to evaluate the functional status of the liver, the kidneys and the blood chemistry. Approximately 500 patients coming from different parts of Nigeria were involved in the testing. Because of the large number of patients involved, three clinics in all were established by NIPRD | to attend to patients involved in the clinical trials.
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From the data obtained it has been determined that the new drug materials of the invention have no detectable adverse affect on the kidney, liver and the blood chemistry. The values recorded for these parameters fall within normal range. On the average, about 90% of the patients involved in the clinical trials have not experienced any major sickle cell disease crisis since they entered the clinical trial program. Even the few patients (10%) who have
AP 0 ϋ U β 9 2 •V' £-Vperienced crises reported that such crises were less freqhent and less severe as compared to their experiences before they joined the clinical trial program.
The patients involved in the clinical trial program had improved appetites accompanied by appreciable weight gain. There toas less incidence of anaemia and jaundice, most likely due/ to the »
reduced rate of destruction of the red blood cells. It was also observed that with patients involved in the clinical trial program whose tests indicated 60 - 90% sickling with sodium metabisulphite at· the beginning of the study, such sickling tendencies had completely disappeared after about 8 months of using the drug materials provided by the present invention. These results are clearly highly encouraging and probative of the efficacy of the drug materials of the invention.
Claims (21)
- V7E CLAIM:1. A composition for treating sickle cell disease comprising a cold water extraction product of a mixture containing Piper guineenses seeds, Pterocarpus osum stem, Eugenia_caryophyllum fruit, and Sorghum bicolor leaves.
- 2. A composition as set forth in claim 1, wherein said mixture comprises potash.
- 3. A composition as set forth in claim 1, wherein said mixture comprises from about 12 to about 17 parts by weight of saidPiper guineenses seeds, from about 15 to about 19 parts by weight *of said Pterocarpus osum stem, from about 12 to about 18 parts by weight of said Eugenia caryophvllum fruit, and from about 25 to about 32 parts by weight of said Sorghum'bicolor leaves.
- 4. A composition as set forth in claim 2, wherein mixture comprises from about 12 to about 17 parts by weight ofPiper guineenses seeds, from about 15 to about 19 parts by weight of said Pterocarpus osum stem, from about 12 to about 18 parts by weight of said Eugenia caryophyllum fruit, from about 25 to about 32 parts by weight of said Sorghum bicolor leaves, and from about 15 to about 22 parts by weight of said potash.
- 5. A composition as set forth in claim 4, wherein said mixture comprises about 15.8 percent by weight of said Piper guineenses seeds, about 18.4 percent by weight of said Pterocarpus osum stem, about 13.2 percent by weight of said Eugenia9 8/012 0 .7U said saidAP«ul»89 2 ( fruryophyllum fruit, about 31.6 percent by weight of said9 Sorahum bicolor leaves, and about 21.0 percent by weight of said potash.
- 6. A composition for preparing a drug effective for treating sickle cell disease comprising a mixture containing Piper guineenses seeds, Pterocarpus osum stem, Eugenia caryophyllum fruit, and Sorghum bicolor leaves. /
- 7. A composition as set forth in claim 6, wherein said mixture comprises potash. ·>
- 8. A composition as set forth in claim 6, wherein said mixture comprises from about 12 to about 17 parts by weight of, said Piper guineenses seeds, from about 15 to about 19 parts by weight of said Pterocarpus osum stem, from about 12 to about 18 parts by weight of said Eugenia caryophyllum fruit, and from about 25 to about 32 parts by weight of said Sorghum bicolor leaves.
- 9. A composition as set forth in claim 7, wherein said mixture^ comprises from about 12 to about 17 parts by weight of saidLpiper- win*ense*seeas' from about 15 to about 19 Ts by wei9ht s of said Pterocarpus osum stem, from about 12 to about 18 parts by weight of said Eugenia caryophyllum fruit, from about<;25 to about 32 parts by weight of said Sorghum bicolor leaves, and from about fc*15 to about 22 parts by weight of said potash. ,
- 10. A Composition as set forth in claim 9, yherein said mixture comprises about 15.8 percent by weight of said Piper guineenses seeds, about 18.4 percent by weight of said PterocarpusAP/P/ 9 8 / 0 1 2 0 7Ai*2 a u 8 9 2OSUKl stem about 13.2 ·· I percent by weight of said * Eugenia caryophyllum fruit, about 31.6 percent by weight of said Sorghum bicolor leaves. and about 21.0 percent by weight of said potash.
- 11. A method for preparing a composition ίor treating sickle cell disease comprising:forming a mixture containing Piper guineenses/' seeds, • *·Pterocarpus osum stem, Eugenia caryophyllum fruit, and gorghtm bicolor leaves; and subjecting said mixture to extraction using cold water to thereby form an extract containing a drug material effective for treating said sickle cell disease.
- 12. A method as set forth in claim 11, wherein said mixture comprises potash.
- 13. A method as set forth in claim 11, wherein said mixture comprises from about 12 to about 17 parts by weight of said Piper guineenses seeds, from about 15 to about 19 parts by weight cf said Pterocarpus osum stem, from about 12 to about 18 parts by weight of said Eugenia carvophyllum fruit, and from about·. 25 to about 32 parts by weight of said Sorghum bicolor leaves.
- 14. A method as set forth in claim 12, wherein said mixture comprises from about 12 to about 17 parts by weight of said Piper gilin .censes seeds, from about 15 to about 19 parts by weight of said pterocarpus osum stem, from about 12 to about 18 parts by weight of said Eugenia caryophyllum fruit, from about 25 to about 32 parts byAP/P/ 9 8 / 0 1 2 07ΑΡύ σ υ ? ?,?ί''/eight of said ^Sorghum bicolor leaves, and from about 15 to about 22 parts by weight of said potash.
- 15. A method as set forth in claim 14, wherein said mixture comprises about 15.8 percent by weight of said Piper guineenses . seeds, about 18.4 percent by weight of said Pterocarpus osum stem, ..about 13.2 percent by weight of said Eugenia carvophyllum/fruit, about 31.6 percent by weight of said Sorghum bicolor leaves, and about 21.0 percent by weight of said potash.
- 16. A method for treating a human afflicted with sickle cell disease comprising:forming a mixture containing Piper guineenses seeds, Pterocarpus osum stem, Eugenia caryophvllum fruit, and Sorghum bicolor leaves;subjecting said mixture to extraction using cold water to thereby form an extract containing a drug material effective for treating said sickle cell disease; and *treating a human afflicted with sickle cell disease with “/said drug material.
- 17. A method as set forth in claim 16 wherein said mixture comprises potash.
- 18. A method asset forth in claim 16, wherein isaid mixture comprises from about 12 to about 17 parts by wcight of said Piper g„uineenses seeds, from about 15 to about 19 part:;, by weight of said Pterocarpus osum stem, from about 12 to about 18 parts by weight ofAP/P/ 9 8 / 0 1 207AF ?Ό ϋ 8 9 2 said Eugenia caryophvllum fruit, and from about 25 to ’about 32 parts,by weight of said Sorghum bicoior leaves.
- 19. * A method as set forth in claim 17, wherein said mixture comprises from about 12 to about 17 parts by weight of said Piper guineenses seeds, from about 15 to about 19 parts by weight of said P.terooarpus osum stem, from about 12 to about 18 parts by weight of said Eugenia carvophyllum fruit, from about 25 to about 32 parts by weight of said Sorghum bicolor leaves, and from about 15 to about 22 parts by weight of said potash.
- 20. A method as set forth in claim 19, wherein said mixture comprises about 15.8 percent by weight of said Piper* guineenses seeds, about 18.4 percent by weight of said Pterocarpus osum stem,
about 13.2 percent by weight of said Euaenia caryophy1lum fruit. about 31.6 percent by weight of said Sorcihum bicolor leaves, and about 21.0 percent by weight of said potash. - 21.» A method as set forth in claim 20 where a dose of approximately 166.7 mg of said drug material is administered orally to said person afflicted with sickle cell diseise.
Priority Applications (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/786,313 US5800819A (en) | 1996-01-25 | 1997-01-21 | Piper guineense, pterocarpus osun, eugenia caryophyllata, and sorghum bicolor extracts for treating sickle cell disease |
ZA9708662A ZA978662B (en) | 1996-01-25 | 1997-09-26 | Phytodrug for treatment and management of sickle cell anaemia and methods of preparing and using same. |
DE19745712A DE19745712A1 (en) | 1996-01-25 | 1997-10-16 | New composition for treating sickle cell disease |
GB9722051A GB2330304A (en) | 1996-01-25 | 1997-10-17 | Pharmaceutical composition for the treatment of sickle cell disease |
FR9713437A FR2770133A1 (en) | 1996-01-25 | 1997-10-27 | New composition for treating sickle cell disease |
CN97122296A CN1217206A (en) | 1996-01-25 | 1997-11-13 | Phytodrug for treatment and management of sickle cell anemia and method of preparing and using same |
APAP/P/1998/001207A AP892A (en) | 1996-01-25 | 1998-01-05 | Niprisan-phytodrug for treatment and management of sickle cell anaemia and methods of preparing and using same. |
JP10101665A JP3053608B2 (en) | 1996-01-25 | 1998-03-11 | Herbal medicine for the treatment and management of sickle cell anemia, its production and use |
Applications Claiming Priority (8)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NG1236996 | 1996-01-25 | ||
ZA9708662A ZA978662B (en) | 1996-01-25 | 1997-09-26 | Phytodrug for treatment and management of sickle cell anaemia and methods of preparing and using same. |
DE19745712A DE19745712A1 (en) | 1996-01-25 | 1997-10-16 | New composition for treating sickle cell disease |
GB9722051A GB2330304A (en) | 1996-01-25 | 1997-10-17 | Pharmaceutical composition for the treatment of sickle cell disease |
FR9713437A FR2770133A1 (en) | 1996-01-25 | 1997-10-27 | New composition for treating sickle cell disease |
CN97122296A CN1217206A (en) | 1996-01-25 | 1997-11-13 | Phytodrug for treatment and management of sickle cell anemia and method of preparing and using same |
APAP/P/1998/001207A AP892A (en) | 1996-01-25 | 1998-01-05 | Niprisan-phytodrug for treatment and management of sickle cell anaemia and methods of preparing and using same. |
JP10101665A JP3053608B2 (en) | 1996-01-25 | 1998-03-11 | Herbal medicine for the treatment and management of sickle cell anemia, its production and use |
Publications (2)
Publication Number | Publication Date |
---|---|
AP9801207A0 AP9801207A0 (en) | 1998-03-31 |
AP892A true AP892A (en) | 2000-11-16 |
Family
ID=33437312
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
APAP/P/1998/001207A AP892A (en) | 1996-01-25 | 1998-01-05 | Niprisan-phytodrug for treatment and management of sickle cell anaemia and methods of preparing and using same. |
Country Status (7)
Country | Link |
---|---|
US (1) | US5800819A (en) |
JP (1) | JP3053608B2 (en) |
CN (1) | CN1217206A (en) |
AP (1) | AP892A (en) |
DE (1) | DE19745712A1 (en) |
FR (1) | FR2770133A1 (en) |
GB (1) | GB2330304A (en) |
Families Citing this family (37)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5800819A (en) * | 1996-01-25 | 1998-09-01 | National Institute For Pharmaceutical Research And Development Federal Ministry Of Science And Technology | Piper guineense, pterocarpus osun, eugenia caryophyllata, and sorghum bicolor extracts for treating sickle cell disease |
US6105991A (en) | 1997-11-20 | 2000-08-22 | The Burton Corporation | Core for a gliding board |
ES2434946T3 (en) | 2005-08-31 | 2013-12-18 | Celgene Corporation | Isoindol imide compounds and compositions comprising them and methods for using it |
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1997
- 1997-01-21 US US08/786,313 patent/US5800819A/en not_active Expired - Lifetime
- 1997-10-16 DE DE19745712A patent/DE19745712A1/en not_active Withdrawn
- 1997-10-17 GB GB9722051A patent/GB2330304A/en not_active Withdrawn
- 1997-10-27 FR FR9713437A patent/FR2770133A1/en not_active Withdrawn
- 1997-11-13 CN CN97122296A patent/CN1217206A/en active Pending
-
1998
- 1998-01-05 AP APAP/P/1998/001207A patent/AP892A/en active
- 1998-03-11 JP JP10101665A patent/JP3053608B2/en not_active Expired - Lifetime
Patent Citations (3)
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US4473559A (en) * | 1981-08-13 | 1984-09-25 | Robinson Larry H | Method and composition for retarding red blood cell sickling |
US5447720A (en) * | 1993-07-06 | 1995-09-05 | Sunday O. Fadulu | Composition and method for treatment of hemoglobinopathies |
US5800819A (en) * | 1996-01-25 | 1998-09-01 | National Institute For Pharmaceutical Research And Development Federal Ministry Of Science And Technology | Piper guineense, pterocarpus osun, eugenia caryophyllata, and sorghum bicolor extracts for treating sickle cell disease |
Also Published As
Publication number | Publication date |
---|---|
CN1217206A (en) | 1999-05-26 |
DE19745712A1 (en) | 1999-04-22 |
US5800819A (en) | 1998-09-01 |
GB2330304A (en) | 1999-04-21 |
JP3053608B2 (en) | 2000-06-19 |
AP9801207A0 (en) | 1998-03-31 |
FR2770133A1 (en) | 1999-04-30 |
JPH11292779A (en) | 1999-10-26 |
GB9722051D0 (en) | 1997-12-17 |
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