AP83A - Substituted tetralins, chromans and related compounds in the treatment of asthma, arthtitits and related diseases. - Google Patents

Substituted tetralins, chromans and related compounds in the treatment of asthma, arthtitits and related diseases. Download PDF

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AP83A
AP83A APAP/P/1988/000106A AP8800106A AP83A AP 83 A AP83 A AP 83A AP 8800106 A AP8800106 A AP 8800106A AP 83 A AP83 A AP 83A
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James Fredrick Eggler
Anthony Marfat
Jr Lawrence Sherman Melvin
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Pfizer
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    • C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
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Abstract

Substituted tetralins, chromans and related compounds

Description

SUBSTITUTED TETRALINS, CHROMANS AND RELATED COMPOUNDS IN THE TREATMENT
OF ASTHMA, ARTHRITIS AND RELATED DISEASES
The present invention is directed to substituted tetralins, chromans and related compounds of the formula (I), depicted below, which by inhibiting
5-lipoxvgenase enzyme and/or blocking leukotriene receptors, are useful in the prevention or treatment of asthma, arthritis, psoriasis, ulcers, myocardial infarction and related disease states in mammals. The present invention is also directed tc pharmaceutical compositions, a method of treatment, and to intermediates useful in the synthesis of said compounds of the formula (I).
Kreft et al., in U.S. Patent 4,661,596, describe compounds which are disubstituted naphthalenes, dihydronaphthalenes or tetralins having the formula
5 wherein the dotted lines represent optional double
5
compounds of the present invention, these compounds inhibit lipoxygenase enzyme and antagonize the effects of leukotriene D4, and so are useful in the prevention and treatment of asthma.
£ 8 0 0 0 0 dV
I
BAD ORIGINAL
-2The chemical nomenclature employed herein generally follows that of I.U.P.A.C. Nomenclature of Organic Chemistry, 1979 Edition, Pergammon Press, New York, 1979·.
Summary of the Invention
The present invention is directed to racemic or optically’active compounds having the structural formula wherein
n is 0 or 1,-
m is 0 or an integer from 1 to 3;
X is ch2, 0, S, SO, S02, NH or N(C^-C^)alkyl;
X1 is CH_ 1 , 0, S, SO or S02;
Y and Y1 are taken together and form a carbonyl
group, or Y and Y1 are taken separately, Y is hydrogen and Y1 is hydroxy or an acyloxy group which is hydrolyzed to form a hydroxy group under physiological conditions;
Z is CH2, CHCH3, CH2CH2 or CH2CH2CH2;
Z1 is CH or N;
R is 2-, 3- or 4-pyridyl, 2-, 3-, 4 or 8-quinolyl, 1-, 3- or 4-isoquinolyl, 3- or 4-pyridazinyl, 3- or
4-cinnolinyl, 1-phthalazinyl, 2- or 4-pvrimidinyl, 2or 4-quinazolinyl, 2-pvrazinyl, 2-quinoxalinyl, 1-, 2or 3-indolizinyl, 2-, 4- or 5-oxazolyl, 2-benzoxazolyl,
3-, 4- or 5-isoxazolvl, 5-benzo[c]isoxazolyl, 3-benzo[d]isoxazolyl, 2-, 4- or 5-thiazolyl, 2-benzothiazoly1,
3-, 4- or 5-isothiazolvl, 5-benzo[c]isothiazolyl,
3-benzo[d]isothiazolyl, 1-[(C^-C^)alkyl]-2-, 4- or
BAD ORIGINAL
-35-imidazolyl, 1-[(C^-C^)alkyl]-2-benzimidazolyl,
1- [(C^-C4)alkyl]-3-, 4- or 5-pyrazolyl, 2-[(C^-C4) alkyl]3(2H)-indazolyl, or 1-[ (C1~C4)alkyl]-3(1H)-indazolyl;
or one of said groups mono- or disubstituted on carbon 5 with the same or different substituents which are bromo, chloro, fluoro, (C^-cy alkyl, trifluoromethyl, hydroxy, hydroxymethyl or (C^-C4)alkoxy, or on adjacent carbons with trimethylene, tetramethylene, -CHj-O-CHj- or -O-CH_-O-; and
R is attached by means of aromatic or heteroaromatic carbon and is phenyl, naphthyl, pyridyl, quinolyl, isoquinolyl, pyridazinyl, cinnolinyl, phthalazinyl, pyrymidinyl, naphthyridinyl, pyrrolyl,
N-[(C^-C^)alkyl]pyrrolyl, indolyl, N-[(C^-C4)alkyl]15 indolyl, isoindolyl, N-[ (C^-C4)alkyl]isoindolyl, indolizinyl, pyrazolyl, 1-[(C^-C^alkyl]pyrazolyl, indazolyl, 1-[(C^-C^)alkyl]-lH-indazolyl, 2-(((^-(^)alkyl]-2H-indazolyl, imidazolyl, 1-[(C^-C4)alkyl]imidazolyl, benzimidazolyl, 1-[(C^-C4)alkyl]benzimidazolyl, furyl, benzofuranyl, isobenzofuranyl, oxazolyl, benzoxazolyl, isoxazolyl, benzo[c]isoxazolyl, benzo[d]isoxazolyl, thienyl, benzothiophenyl, isobenzothienyl, thiazolyl, benzothiazolyl, isothiazolyl, benzo[c]isothiazolyl, or benzo[d]isothiazolyl; or, only when either χ1 is CH2 or m is at least 2, is attached by means of heterocyclic nitrogen and is 1-pyrrolyl, 1-indolyl,
2- isoindolyl, 1-pyrazolyl, 1(1H)-indazolyl, 2(2H)indazolyl, 1-imidazolyl or 1-benzimidazolyl; or R1 is one of said groups which is mono- or disubstituted on carbon with the same or different groups which are bromo, chloro, fluoro, hydroxy, hydroxymethyl, (C^-C4) alkyl, (C^-C4)alkoxy, carboxy, [ (C^-C4) alkoxy]carbonyl, or substituted on adjacent carbons with trimethylene, tetramethylene, -Ct^-O-CHj- or -O-CI^-O-;
or substituted on tertiary nitrogen to form an N-oxide;
APO00083
BAD ORIGINAL
-4-i
Vba pharmaceutically acceptable acid addition salt thereof; or a pharmaceutically acceptable cationic salt when the compound contains a carboxy group.
Eecause of their ease of preparation and valuable biological activity, in the preferred compounds of the formula (I), regardless of the value of Y and Y^, n is 1, m is 0, X and are each independently CH9 or Ο, Z is CH2, Z is CH, R is 2-, 3- or 4-pyridyl, 2-quinolyl,
6-fluoro-2-quinolyl, 5-fluoro-2-benzothiazolyl or
2-pyrazinyl, and R* is phenyl, 3-methoxyphenyl,
4-methoxyphenyl, 3-methoxycarbonvlphenyl, 4-methoxycarbonylphenyl, 3-carboxypheny1, 4-carboxyphenyl, 2-pyridyl or 3-pyridyl.
In the most preferred compound when Y and Y^ are taken together to form a carbonyl group, n is 1, m is Ο, X is 0, X1 is CH2, Z is CH2, Z1 is CH, R is 2-quinolyl and is 3-pyridyl.
When Y is H and Y^ is OH, most preferred are racemic or optically active compounds having the relative stereochemical formula or
— (II)
most particularly those racemic or optically active compounds of the formula (II) or (III) wherein· X and
BAu HGINAL
-5X are each 0 or CH_, R is 2-quinolyl, 6-fluorc-2z 1 quinolyl or 5-fluoro-2-benzothiazolyl, and R is
3-pyridyi, 3-carboxyphenyl or 4-methoxypheny1.
Said pharmaceutically-acceptable acid addition 5 salts include, but are not limited to, those with HC1,
HBr, HNO3, H2SO4, H3PO4, CH3SO3H, £-CH3CgH4SO3H,
CH3CO2H, gluconic acid, tartaric acid, maleic acid and succinic acid. In the case of those compounds of the formula (I) which contain a further basic nitrogen, it will, of course, be possible to form diacid addition salts (e.g., the dihydrochloride) as well as the usual monoacid addition salt. Said pharmaceutically-acceptable cationic salts include, but are not limited to, those of sodium, potassium, calcium, magnesium, ammonia,
Ν,Ν'-dibenzylethylenediamine, N-methylglucamine (meglumine), ethanolamine and diethanolamine.
The reference to Y3- as an acyloxy group which is hydrolyzed to a hydroxy group under physiological conditions refers to esters of a type which are frequently referred to as pro-drugs. Such esters are now as well-known and common in the medicinal art as pharmaceutically-acceptable salts. Such esters are generally used to enhance oral absorption, but in any event are readily hydrolyzed in vivo to the parent hydroxy compound. The more preferred acyloxy groups are those in which the acyl moiety is the alpha-aminoacy1 residue of a naturally occurring L-alpha-amino acid,
-C-(CH2)p
NRZR3
-C-(CH2)rCOOH,
APO 00 0 8 3
O
-C-CKNH-(CH-) NR2R3, 2 2 q or -C-CHNH-(CH-.) COOH; 2 2 S wherein
BAD ORIGINAL ft
-62 3
R and R are taken separately and are each
1 independently hydrogen or (C^-C^) alkyl, or R and R are taken together with the nitrogen to which they are attached to form a pyrrolidine, piperidine, perhydro-
5 azepin or morpholine ring;
P is an integer from 1 to 4;
q is an integer from 1 to 3;
r is an integer from 2 to 3; and
s is an integer from 1 to 3 .
10 Also forming a part of the present invention are
pharmaceutical compositions for administration to a
JB mammal which comprise a compound of the formula (I) and a pharmaceutically acceptable carrier; and a method of inhibiting 5-lipoxygenase enzyme and/or blocking leukotriene D4 receptors in a mammal, so as to prevent or treat asthma (particularly in man), arthritis, psoriasis, gastrointestinal ulcers, or myocardial infarction.
Finally, the present invention is directed to valuable intermediate compounds having the structural formula
R
R — (IV) wherein
n.
X, Z and Z are as defined above;
3
Y and Y are taken together and form a
3 2 group, or Y and Y are taken separately, Y v3 carbonyl is hydrogen and Y is hydroxy; and a
R is hydroxy or benzyloxy;
&Αύ ORIGINAL ft and RC is -X1- (CH-) -R1
I2 m m, X *-J
R and R are taken separately and R is hydrogen and and R- are as defined above;
or in the second alternative b π
R and R' are taken together and are hydroxyh c methylene or diazo; or R and R are taken separately, b c
R is hydrogen and R is bromo;
Ra is rJ
-0·^ ; and
R^ is phenyl or a value of R as defined above.
The preferred values of n, m, X, X1, Z, Z1, R and are also as defined above.
The present invention is readily carried out. Without regard to geometrical (cis-trans) or optical isomers, the compounds of the formula (I) wherein
Y + Y1 = carbonyl, or Y = H and Y1 = OH, and X1 = CH2,
S or 0 are prepared according to the chemical transformations which are summarized in Flowsheets 1, 2 and 3, where the symbols n, m, X, Z, Z^, R and R^ are as defined above. The various transformations found in these flowsheets, as well as transformations required for the preparation of the compounds (I) having other values of Y, Y^ and X^, and methods for separation of cis-trans and optical isomers, are detailed below.
The condensation of Flowsheet 1 is typically carried out with the phenolic group in protected form as shown, methyl being a preferred protecting group only when Xx is CH2· The preferred conditions employ a molar excess of the required aldehyde and a molar excess of a secondary amine such as pyrrolidine or piperidine as base. (It is understood that such a base facilitates the condensation by forming an enamine intermediate .)
APO 00 0 8 3
BAD ORIGINAL
-8Flowsheet 1 When X1 = CH.
(I)
Y+Y1=carbonyl
X1=CHO
Ο
Condensation
C-Alky la-t^ion
X2CHo(CHo) r1 2 2 m
Catalytic
Hydrogenation n R (CH~) CHO 2 m
Catalytic Hydrogenation
R—benzyl
CH-(CH_) R 2 2 m
R=C6H5CH?
(IV) in the first (IV) in the first
alternative A alternative
Ra= benzyloxy Ra= hydroxy
Y2+Y'3=carbonyl YZ+YJ-carbonyl
xL= ck2 x1= ch2
f.3=r, CH3, or C6H5CH2
X2=Nucleophically displaceable group such as I, Br, Cl, CH^SO^ or £-CH3C6H4SO3 bad original 4
-9Flowsheet 2 When χΐ = 0 or S
Phenolic
Alkylation r6ch2x2
Formylation (IV) in the second alternative 2 3
Y +Y =carbonyl b c
R +R =hydroxymethylene X1=0 or S
Bromination £-ch3c^r4n? (IV) in the second alternative
3
Y +Y =carbonyl Rb+Rc=diazo X1=0 or S (a) r6=r (I)
Y+Y^=carbony1 X1=0 or S
(IV) in the second alternative
Y2+Y2=carbonyl Rb=H, RC=Br X1=0 or S (b)
R6-c6H5 (IV) in the first alternative
Ra=benzyloxy Y2+Y2=carbonyl X1=0 or S
R =R or
X2=C1, 3r, I, CH3SO3, £-CH3CgH4S03or other nucleophilically displaceable group (a) RL(CH_) SH or R1(CH?) OH, z in m m rhodium (II) acetate dimer (b) R1(CH_) SH or R1(CH_) OH, base m m £ 8 0 0 0 0 dV
BAD ORIGINAL 4
-10j· fi qfi
Flcwsheet 3 When X^ = CH?, 0 or S (I)
Y+Y^=carbony1 X1» CH,, O or S
Phenolic Alkylation
Reduction (I)
Y=H, Y1=OH
X1 = CK2, O or S
X
Phenolic Alkylation (IV) in the first alternative
Ra=benzyloxy Y^+Y^=carbonyl X1=CH2, 0 or S
BAD ORIGINAL &
The reaction is generally carried out in a reactioninert solvent, lower alcohols such as methanol being particularly well suited for this purpose. The temperature conditions for this transformation are not critical, e.g., 0-70° C. is generally satisfactory, with ambient temperature particularly well suited as a matter of convenience.
As used here and elsewhere herein, the expression reaction-inert solvent refers to a solvent which does not interact with starting materials, reagents, intermediates or products in a manner which adversely affects the yield of the desired product.
The C-alkylation of Flowsheet 1 is carried out by first converting the ketone (A) to its lithium salt, usually in situ, by the action of substantially one molar equivalent of a strong, sterically hindered base such as lithium diisopropylamide, usually carried out at low temperature (e.g., about -40 to -80° C. conveniently at the temperature of a dry ice-acetone bath).
The salt in turn is reacted with the alkylating agent, preferably the highly reactive iodide, usually in molar excess in the presence of a molar excess of hexamethvl phosphcramide, now at higher temperature (e.g., about 0 to 40° C.). Conveniently, the latter reagents are added to the cold lithium salt solution, and the temperature allowed to rise to ambient temperature as the reaction proceeds. The salt preparation and alkylation reaction are usually carried out in the same reactioninert solvent (e.g., tetrahydrofuran). It will be evident to those skilled in the art that any free hydroxy or carboxy groups in the alkylating reagent should be in protected form (vide supra).
ΔΡ0 0 0 0 8 3
BAD ORIGINAL ft
-12The catalytic hydrogenation transformations (debenzylations, ^-additions to double bond) of Flowsheets 1, 2 and 3 are carried out under conventional conditions, generally in a reaction-inert solvent, and preferably using a noble metal catalyst and moderate conditions of temperature (e.g., about 0 to 70° C.) and hydrogen pressure (e.g., about 1 to 10 atmospheres). While higher pressures may t>e desirable in selected instances, such moderate pressures permit the use of much less elaborate and expensive equipment. Suitable noble metal catalysts include platinum, palladium, rhenium, rhodium and ruthenium, either of the supported '·'·' or non-supported type, as well as the known catalytic r··'· compounds thereof such as the oxides, chlorides, etc.
Examples of suitable catalyst supports include carbon, .- silica and barium sulfate. The catalysts may be preformed or formed in situ by prereduction of an appro,0 priate salt of the catalytic compound. Examples of preferred catalysts are 5% palladium-on-carbon, 5% platinum-on-carbon; 5% rhodium-on-carbon, platinum chloride, palladium chloride, platinum oxide and ruthenium oxide. Most preferred in the present instance is pailadium-on-carbon. Solvents generally suitable for the present hydrogenation include lower alkanols, ethyl acetate and tetrahydrofuran.
The methyl ethers [compounds of the formula (C)] in Flowsheet 1 are deblocked to form the corresponding phenol derivative, again, by conventional methods; for example, using concentrated K3r, or 3Br^, both of which are exemplified below.
BAD ORIGINAL ft
-13The phenolic alkylations found in Flowsheets 2 and 3 and the bromine replacement reaction of Flowsheet 2 each represent conventional nucleophilic displacement reactions. These displacements are generally carried out in the presence of a base of sufficient strength to convert the displacing phenol, alcohol or thiol to its salt, and in a quantity at least sufficient to neutralize 2 the by-product acid (HX , HBr). In those substrates which contain an aliphatic alcohol group [e.g., a com2 3 pound (IV) wherein Y is H and Y is OH], bases of sufficient strength to convert that group to the anion will generally be used in an amount no more than sufficient to convert the more acidic phenol to the salt. When either of the reactants contains a group of acidity similar to or greater than that of the nucleophilic displacing compound, such potentially interfering groups are best introduced in protected form (e.g., a heteroaromatic phenolic group as methoxy or benzyloxy, a carboxy group as methyl or benzyl ester, removable by hydrolysis or hydrogenolysis according to methods detailed elsewhere herein). The present nucleophilic displacements are carried out in a reaction-inert solvent, preferably one which is much less acidic than the displacing phenol, alcohol or mercaptan. Most preferred are polar, aprotic solvents such as dimethylformamide or acetone, usually with a molar excess of the more readily available of the two reactants. Temperature is not critical, e.g., about 10-70° C. is usually satisfactory with ambient temperature most convenient. In one preferred variant, the phenol, alcohol or mercaptan is irreversibly converted to the anion with a base such as sodium hydride. Other preferred variants employ X^CO^ as base in the presence of Nal, or Cs2CO3 as base in the presence of Csl.
£ 8 0 0 0 0 dV
BAD ORIGINAL
-14In the special case of X=NH, such nucleophilic displacements will generally be carried out with the NH group protected, e.g., as the N-benzvl derivative (subsequently removed by hydrogenation) or as an N-alkanoyl or N-sulfonyl derivative (subsequently removed under appropriate hydrolysis conditions; for example, the N-tosyl derivative is hydrolyzed by heating in a mixture of acetic acid and concentrated HCl).
The formylation of Flowsheet 2 represents a 10 conventional condensation type reaction of a ketone with an alkyl formate. This reaction is generally in an aprotic reaction-inert solvent such as toluene in the presence of a strong base such as sodium hydride at moderate temperatures (e.g., 0-70’ C., conveniently at ambient temperature). The subsequent conversion to the diazo compound is conveniently accomplished with tosyl azide as the reagent, a reaction generally carried out at low temperature (e.g., about -10 to -60° C.) in the presence of molar excess of a tertiary amine (e.g., triethylamine) in a reaction-inert solvent such as
CH2CI2· In turn, the diazo compound is reacted with an appropriate alcohol or mercaptan in the presence of a catalytic amount of rhodium (II) diacetate dimer to form the desired ether or thioether. The latter trans25 formation is generally carried out in an anhydrous reaction-inert solvent such as toluene at somewhat elevated temperature, e.g., about 50-100° C. Substituent alcohol or carboxy groups which are not intended to react are preferably protected in this transformation, as in the case of the nucleophilic displacement reactions discussed above.
BAD ORIGINAL &
-15The reduction reactions of Flowsheet 3 require the reduction of a ketone to a secondary alcohol, for which a number of selective reagents are available.
Where no other LiAlH^ reducible groups (such as carboxy, methoxycarbonyl) are present, that reagent is well suited for this purpose. On the other hand, NaBH^ is preferred as the reducing agent when such reducible groups are present. In either case, these hydride reductions are generally carried out in a reaction-inert solvent (such as tetrahydrofura.n in the case of LiAlH^, methanol or a combination of methanol and tetrahydrofuran in the case of NaBH^). In either case, temperature is not critical, about 0 to 50° C. being generally satisfactory and ambient temperature preferred. The present reduction step offers the potential of producing a mixture of cis- and trans-isomers [as illustrated in the formulas (II) and (III)] and in the present hydride reduction, that is the result which is generally observed. If one or the other of these isomers is particularly desired, one can usually find a reduction method and set of conditions which will favor the desired isomer.
For example, NaBH, reduction in the presence of cesium
AP 0 0 0 0 8 3 chloride will generally strongly favor the cis-isomer. Catalytic hydrogenation is also a generally useful reduction method, generally carried out under conditions which are somewhat more vigorous than those described above (e.g., more prolonged time, higher catalyst level, higher temperature and/or higher pressure). Hydrogena. tion is preferably carried out on substrates such as
HO
R — (V)
BAD ORIGINAL a
3» which contain no other readily hydrogenated group.
?d/C catalyst tends to particularly favor formation of cis-isomer. However, by variation of the catalyst and conditions, it will be possible to modify or even reverse that tendency. Where both cis- and trans-isomers form in the present reduction, they are generally separable by standard chemical methods (e.g., selective or fractional crystallization, chromatography, and so forth).
If compounds wherein X^- is SO or S02 are desired, they are usually prepared from the corresponding compounds of the formula (I) or (IV) wherein the group χΐ as S is already in place. Peroxides are generally used as oxidizing agent. A particularly convenient reagent for this purpose is m-chloroperbenzoic acid.
The sulfide is reacted with substantially 1 molar equivalent of this reagent to obtain the sulfoxide and with at least 2 molar equivalents to obtain the sulfone, in a reaction-inert solvent such as CH2C12. Temperature is not critical, e.g., 0-60° C. being generally satis20 factory and ambient temperature preferred. However, when X is S, and compounds wherein X^ is SO or SO2 are desired, these are preferably formed by conventional sulfinylation or sulfenylation of an unsubstituted ketone compound of the formula (A), (D) or (E).
Those ketone compounds of the formula (I) wherein
Y and γΐ form a carbonyl group, and of the formula (IV) in the first alternative, contain an asymmetric carbon at the alpha-position which is adjacent to the carbonyl group, and therefore are racemic compounds capable of resolution into optically active enantiomers, e.g., by conversion of the racemate into diastereomeric salts with an optically active acid, which are generally, separable by a fractional crystallization process. Alternatively, if the substrate contains a carboxy
BAD ORIGINAL ft
-17group, separable diastereomeric salts are formed with an optically active organic amine. Optical activity can also be induced by use of an optically active reagent in the step by which the asymmetric carbon is formed,
e.g., use of an optically active Wilkinson type catalyst, or a noble metal supported on an optically active support, in the hydrogenation step. The optically active ketones are also available by conventional reoxidation of an optically active alcohol of the next paragraph, e.g., via the Jones oxidation, which is exemplified below.
The hydroxy compounds of the formula (I) and (IV) wherein Y (or Y^) is hydrogen and Y^ (or Y^) is OH contain two such asymmetric carbons—corresponding to two racemates and four optically active compounds. One of these racemates is the above noted cis-isomer, and the other the trans-isomer. Each of these racemates is capable of resolution into a pair of enantiomers via diastereomeric salts, as detailed in the preceding paragraph. It is preferred, however, to convert the racemic alcohol to corresponding diastereomeric esters or urethanes formed with an optically active acid or isocyanate. Such covalently bonded derivatives are generally subjectable to a broader variety of separation methods (e.g., chromatography) than are diastereomeric salts. Such diastereomeric esters are formed from the alcohol and the optically active acid by standard methods, generally those involving activation of the acid, e.g., as the acid chloride, as a mixed anhydride with an alkyl chloroformate, or with a dehydrative coupling agent such as dicyclohexylcarbodiimide. A preferred optically active acid in the present case is S-O-acetylmandelic acid. Once the resulting diastereomeric esters are separated, e.g., by chromatographic methods, they are hydrolyzed by conventional methods, e.g., aqueous acid or aqueous base, to obtain the enantiomeric, optically active alcohols.
AP 0 0 0 0 8 3
BAD ORIGINAL &
-18The prodrug esters of the present invention are prepared by methods similar to those used in the synthesis of esters in the preceding paragraph. Esters with alpha-amino acids, including natural L-amino acids, will generally by prepared from the appropriate ainino acid in which the alpha-amino group, substituent NH2 or NH groups (e.g., lysine, ornithine, arginine, histidine, tryptophan), hydroxy groups (serine, homoserine, threonine, tyrosine), mercapto groups (cysteine) and substituent carboxy groups (glutamic acid, aspartic acid) are in protected form (e.g., N-benzyloxycarbony1, 0- and S-benzvl) generally removed by catalytic hydrogenation in a subsequent step. Similarly, in the case of esters with primary or secondary amino substituents, the acids will be coupled with amino groups protected. Such protection is, of course, unnecessary with those acids containing tertiary amino substituents. Finally, the carboxy substituted esters are most conveniently prepared from the cyclic anhydride:
O
Concerning the biological activity of the present compounds, it is known that arachidonic acid is metabolized in mammals by means of two distinct pathways, one leading to prostaglandins and thromboxanes, the other to several oxidative products called leukotrienes, which are designated by letter number combinations such as B4, C4 and D4. The first step in this oxidative pathway is the oxidation of arachidonic acid under the influence of 5-lipoxygenase enzyme, an enzyme which is generally inhibited by the compounds (I) of the present invention, thus blocking the synthesis of all leukotrienes. That in itself provides the mechanism sufficient
BAD ORIGWL
-19for the utility of the present compounds in the treatment or prevention of asthma (where LTC4 and LTD4 are understood to be mediators) , arthritis (where LTB4 is understood to be a mediator in inflammation) , psoriasis (where LTB4 is understood to be a mediator) , ulcers (where LTC4 and LTD4 are understood to be mediators) and myocardial infarction (where LTB4 is understood to be a mediator). Supplementing this enzyme inhibitory activity is the general ability of the present compounds to antagonize leukotriene 04 (i.e., block LTD4 receptors). In general, the present compounds also antagonize leukotriene B4. For a review concerning leukotrienes, see Bailey et al., Ann. Reports Med. Chem. 17, pp. 203-217 (1982) .
The in vitro activity of the compounds of the formula (I) is tested as follows. RBL-1 cells, maintained in monolayer form are grown for 1 or 2 days in spinner culture in Minimum Essential Medium (Eagle) with Earl’s Salts plus 15% Fetal Bovine Serum supple20 mented with antibiotic/antimycotic solution (GIBCO).
The cells are washed 1 time with RPMI 1640 (GIBCO) and resuspended in RPMI 1640 plus 1 microM glutathione to a cell density of 1 x 10 cells/ml. A volume of 0.5 ml of the cell suspension is incubated at 30° C. with 0.001 mi of dimethyIsulfoxide solution of drug for 10 minutes.
The reaction is started by a simultaneous addition of 0.005 ml (14C)-arachidonic acid in ethanol and 0.002 ml A23187 in dimethylsulfoxide to give final concentrations of 5.0 and 7.6 microM, respectively. After a 5 minute incubation at 30° C., the reaction is stopped by the addition of 0.27 ml acetonitrile/acetic acid (100/0.3) and the media is clarified by centrifugation. Analysis of the product profile is made by a 0.2 ml injection of the clarified supernatant into HPLC. The separation of
AP 0 0 0 0 8 3
BAD ORIGINAL
-20radioactive products is effected on a radial ?AX CN column (5 mm I.D., Waters) with a solvent system cf acetonitrile/H^O/acetic acid (0.1%) with a linear acetonitrile gradient from 35% to 70% over 15 minutes at 1 ml/minute. Quantitation is accomplished with a
Berthold Radioactivity Monitor equipped with a built-in integrator and a 0.2 ml flow cell mixing 2.4 ml/minute Omnifluor (NEN) with column effluent. Integration units for each product are calculated as a percentage of total integration units, and then compared to the average control levels. The results are expressed as Percent of Control and are plotted vs the log of drug concentration. The IC-θ values are estimated by graphical inspection.
The leukotriene D4 (LTD4) receptor assay tests the ability of a compound to compete with radiolabelled LTD4 for specific LTD4 receptor sites on guinea pig lung membranes. In this test, normal 3-4 week-old guinea pigs are acclimatized under standard conditions for 3 days prior to being sacrificed. Final animal age; 24-31 days. The guinea pigs are stunned by a blow to the back of the neck, and exsanguinated by cutting the carotid artery. The chest cavity is opened and the lungs are removed, rinsed in 50 mM Tris buffer (pH 7.0) and placed in clean buffer. In this and all subsequent operations, all tissue and buffer are kept on ice throughout the preparation, and all centrifugation is carried out at 4° C. Bronchi and connective tissue are trimmed from the lungs. The tissue is weighed and placed in 50 ml polycarbonate tubes with buffer at a ratio of 1 gm tissue/3 ml buffer. The tissue is homogenized by a Tekmar Tissumizer at full
BAD ORIGINAL ft
-21- .
speed for 30 seconds and centrifuged in a Scvall SS-34 rotor at 3250 rpm x 15 minutes. The supernatant is centrifuged at 19,000 rpm x 10 minutes. The resulting pellet is resuspended in buffer with the Tissumizer at medium speed (position 75) for 10 seconds. Tl\£ resuspension is again centrifuged at 19,000 rpm x 10 minutes. The resulting pellet is resuspended by the Tissumizer at slow speed (position 50) for 10 seconds in 1 ml buffer/g of starting tissue. This final suspension is stirred at 4° C. while aiiquoted to polypropylene tubes and stored at -70° C. The following are added to a 12x75 mm polystyrene tube:
(1) 25 microL of one of the following:
A. Dimethylsulfoxide (to determine total binding) microM LTD4 (to determine non-specific binding) nanoM - 100 microM compound in dimethylsulfoxide
0.025 ml 3H-LTD4 (specific activity 30-60 Ci/mmol) in 50 mM Tris (pH 7.0) + 10 microM L-cvsteine (12,000 - 15,000 cpm/0.025 ml)
0.2 ml diluted membrane preparation (1 mg/ml) (The preparation is diluted in 50 microM Tris buffer + MgCi2 such that in 200 microL protein, a 10 microM MgCl2 concentration is achieved).
The reaction tubes are incubated at 25° C. for 30 minutes. Four ml of cold Tris buffer + 10 microM MgCl2 are added to each tube. The contents are quickly filtered through a Whatman GF/C filter with a Yeda separation device. The filter is washed 3X with 4 ml The filter is transferred to a Uitrafluor scintillation fluid is (2) (3)
B.
C.
AP000083
Tris-MgCl2 buffer, scintillation vial.
BAD ORIGINAL
-22added. The vial is capped, vortexed and counted for 3 hours. Percent specific binding is calculated using the formula:
% SB = (X - NSB)/(TB - NS3) , where X = cpm sample
NSB = cpm non-specific binding
TB = cpm total binding
Percent specific binding is graphed as a function of compound concentration. IC5q is that concentration at which 50% SB occurs. Ki is calculated by using the formula :
Ki = (IC5Q)/(1 + (L/Kd)] , where L = concentration of ligand added (microM) = cpm Or- added/cpm of 1 microM 3H-LTD4 c- 15 Kd = 1 microM (dissociation constant)
Human polymorphonuclear leukocytes are employed to measure the competition of test molecules with [3H]-LTB4 for binding at the LTB4 receptor. In this test neutrophils are isolated from heparinized human peripheral blood (usually 100 ml) using- a Hypaque-Ficoll gradient (density 1.095 g/ml). Hanks balanced salt solution (HBSS) containing 0.1 grams/100 ml bovine serum albumin (HBSS-BSA) is used to resuspend the cells. The one step Hypaque-Ficoll technique yields highly pure popula25 tions of neutrophils (greater than 95%). Cell viability is assessed by trypan blue dye exclusion (should be greater than 95%), and the functional integrity of the neutrophils was determined by nitroblue tetrazolium reduction (should be greater than 85% positive).
Compounds undergoing test are dissolved in dimethylsulfoxide at a concentration of 100 microM. These solutions are diluted by a factor of 500 using HBSS-3SA. A concentration of 100 microM drug is achieved by introducing the diluted sample in a 0.5 ml aliquot into the reaction
BAD ORIGINAL
-23tube. Serial· dilutions of 1-3 and 1-5 are made (as appropriate) and a 0.5 ml aliquot of these dilutions is added to the incubation tube. [3H]-LT34 (NEN:specific radioactivity, greater than 180 Ci/mmol; 0.005 ml in absolute ethanol) is introduced into borosilicate tubes (12 x 75 mm). A volume of 0.5 ml of the drug solution (see above) is then added. The binding reaction is initiated by adding 0.5 ml of ice cold neutrophils at a cell density of [5 x 10^ cells/mll, and continued at
4’ C. for 30 minutes. The incubation is terminated by rapid filtration through a Whatman GF/C glass filter to separate the free from the bound radiolabelled ligand.
The filters are washed 3-times with 3 ml ice-cold H3SS, dried, placed in 4 ml of Ultrafluor, and counted.
Total binding is defined as the CPM present on the filter (cell associated) when radiolabelled ligand is incubated with neutrophils in the absence of any competing agent. Nonspecific binding is obtained by incubating cells with radiolabelled ligand plus 1 microM nonradiolabelled LT34 . Specific binding is total binding CPM corrected for the nonspecific binding CPM. Every tube is corrected for nonspecific binding. Points of half-maximal displacement of radiolabelled ligand are estimated by graphical analysis on a semi-logarithmic plot of percent of specific binding (no competitor present) vs concentration.
To evaluate the compounds of the formula (I) in vivo, they are tested by the so-called PAF lethality assay procedure:
Materials:
Mice: CD1 males, all approximately the same weight (approximately 26 grams), 12 per group.
AP 0 0 0 0 8 3 bad original &
-24‘•«w*
X
Vehicle for oral drug dosing: EES (5% ethanol, 5% etr.ulphor, 90% saline). Stored at room temperature .
Drugs: For routine screening at 50 mg/kg, 20 mg drug is dissolved in 4 ml EES, using sonication in a sonicator bath or grinding in a Ten Broeck grinder to dissolve drug if necessary. If solubility is still a problem, the drug is used as a suspension.
Vehicle for i.v. Injection: Saline with 2.5 mg/ml
Bovine Serum Albumin (BSA, Sigma #A4378) and 0.05 mg/ml Propranolol (Sigma 4P0884). Prepared fresh daily and kept at room temperature .
Platelet Activating Factor (PAF): A 10 microM stock solution is prepared by dissolving 1 mg PAF (Calbiochem #429460) in 0.18 ml ethanol. This is stored at -20° C. and is diluted in vehicle (see above) the day of use. The concentration of PAF used is calibrated so that when injected at 0.1 ml/10 grams body weight, it will kill approximately 80% of untreated controls. This is usually about 0.028 g/kg (a 1 to
2034 dilution from stock). The solution is prepared in glass containers and is used with glass syringes to minimize surface adhesion by the PAF. It is kept at room temperature.
Positive Control: Phenidone is used at 25 mg/kg (its approximate ED 50).
BAD ORIGINAL
-25Method:
minutes before PAF injection, mice are treated orally with drug using 0.1 ml/10 grams body weight. 35 to 40 minutes later they are placed under a heat lamp to dilate the caudal vein for PAF injection. PAF is injected i.v. at 0.1 ml/10 grams body weight, and death follows usually within 30 minutes, rarely after 60 minutes. Results are expressed as percent mortality as compared tc controls. Because the assay appears to be sensitive to endogenous catecholamines (i.e., beta agonists protect the mice), Propranolol is used to overcome this potential problem. It also helps if the mice are acclimated to the room before testing, and if room noise and temperature are kept moderate and constant. The heat lamp distance should be calibrated so as tc permit vasodilation without visible stress to the mice. Fasting the mice should be avoided. Variations :
1. The time for oral dosing can be changed.
2. Intravenous drug dosing is possible by coinjecting the drug with PAF in the same volume and vehicle as described above. For coinjection, PAF is prepared at twice the desired concentration in saline with BSA and Propranolol as above, and the drug is prepared at twice the desired concentration in the same vehicle. The two preparations are mixed in equal volumes immediately before injection.
For use in the prevention or treatment of asthma, arthritis, psoriasis and gastrointestinal ulcers in a mammal, including man, a compound of the formula (I) is given in a 5-lipoxygenase inhibiting and/or leukotriene receptor blocking amount of about 0.5-50 mg/kg/day, in single or divided daily doses. A more preferred dosage range is 2-20 mg/kg/day, although in particular cases,
AP 0 0 0 0 8 3
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-26at the discretion .of the attending physician, doses outside the broader range may be required. The 'preferred route of administration is generally oral, but parenteral administration (e.g., intramuscular, intra5 venous, intradermal) will be preferred in special cases, e.g., where oral absorption is impaired as by disease, or the patient is unable to swallow.
The compounds of the present invention are generally administered in the form of pharmaceutical compositions comprising at least one of the compounds of the formula (I), together with a pharmaceutically acceptable vehicle or diluent. Such compositions are
X generally formulated in a conventional manner utilizing solid or liquid vehicles or diluents as appropriate to the mode of desired administration: for oral administration, in the form of tablets, hard or soft gelatin capsules, suspensions, granules, powders and the like; and, for parenteral administration, in the form of injectable solutions or suspensions, and the like.
The present invention is illustrated by the following examples, but is not limited to the details thereof .
-27EXAMPLE 1
6-Methoxy-3- ( 3-pyridyl) methy lene-4-ch'romanone
To a 25° C. mixture of 20.0 g (0.112 mol) of
6-methoxy-4-chromanone and 13.09 g (0.169 mol) of
3-pyridinecarbaldehyde in ICO ml of methanol was added 14.1 ml (0.169 mol) of pyrrolidine. The resultant solution was allowed to stir 60 hours at 25’ C. , cooled to 0° C. and filtered to yield 17.07 g (57%) of the title compound, m.p. 127-131° C.
MS (m/e) 267 (M~) , 23S, 161 , 150 (100%), 135 and
107. IR (CHC13) 1671 (C=O), 1614, 1589 aftd 1566 cm'1. 1H-NMR(CDC13)delta(ppm): 3.79 (s, OCHj), 5.23 (d,
J=1.5 Hz, CH,), 6.86 (d, J=8 Hz, C-8H), 7.06 (dd, J=8,
Hz, C-7H), 7.37 (d, J=1.5 Hz, vinyl H), 7.36, 7.58,
7.75, 8.52 and 8.57 (multiplets, 5ArH).
Analysis calculated for ci5^i3NO3:
C, 71.90; H, 4.90; N, 5.24%.
Found: C, 71.72; H, 4.85; N, 5.16%.
EXAMPLE 2
0 6-Methoxy-3- (3-pyridylmethyl) -4-c'nromanone
A mixture of 25.2 g (94.4 mmol) of the title product of the preceding Example and 2 g of 5% Pd/C/50% H^O in 1 liter ethyl acetate was hydrogenated at 35 psig hydrogen for 18 hours. The reaction was filtered through diatomaceous earth with ethyl acetate wash, and the combined filtrate and wash evaporated to an oil. Trituration of this oil with diisopropyl ether gave the title compound as crystals, m.p. 82-84° C.
MS (m/e) 269 (M+) , 252 , 177 , 150 (100%), 135, 118 and 107. IR (CHCl-j) 1635 (C=O) , 1618 and 1578 cm1. 1H-NMR(CDC13)delta (ppm) : 2.71 (dd, J=15, 10 Hz, 1
CH2Ar), 2.86 (m, CH), 3.19 (dd, J=15, 6 Hz, lCH2Ar), 3.75 (s, OCH3), 4.07 (dd, J=ll, 8 Hz, 1CH2O), 4.30 (dd, J=ll, 6 Hz, 1CH2O), 6.82 (d, J=9 Hz, C-8H), 7.03 (dd,
AP 0 0 0 0 8 3
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-2810
C, 71.13; Η, 5.57; N, 5.12%. Found; C, 71.31; H, 5.58; N, 5.15%.
EXAMPLE 3
6-Hydroxy-3-(3-pyridylmethyl)-4-chromanone
A mixture of 13.75 g (51.1 mmol) of the title product of the preceding Example, 46 ml of concentrated hydrobromic acid and 47 ml of acetic acid was heated at reflux for 10 hours, and then stirred 12 hours at 25° C. The reaction was poured into 470 ml of ice and water and the pH adjusted to 7.5-8 with solid sodium bicarbonate. The precipitate formed was stirred 0.5 hours, filtered, washed with water and dried in vacuo to yield 11.79 g (90%) of the title compound, m.p. 163-166° C.
MS (m/e) 255 (M+, 100%), 241, 163, 136, 120 and 108. IR(KBr) 1687 (C=O), 1625, 1598 and 1582 cm1. 1H-h74R (DMSO-dg ) delta (ppm) : 2.65 (dd, 3=11, 17 Hz, lCH2Ar), 3.10 (m, CH and lCH2Ar), 4.11 (dd, J=ll, 11 Hz, 1OCH2), 4.27 (dd, 3=11, 5 Hz, lOCHp, 6.85 (d, 3=8 Hz, C-8H), 6.98 (dd, 3=8, 2 Hz, C-7H), 7.07 (d, 3=2 Hz, C-5H), 7.31 (dd, 3 = 9, 8 Hz, C-5 PyrH), 7.67 (d, J=8 Hz, C-4 PyrH), 8.42 (m, 2 PyrH) and 9.48 (s, OH).
Analysis calculated for C^H^NOy iH2O:
C, 69.35; H Found: C, 69.39; H
5.39
5.08; N, 5.37%
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-29EXAMPLE 4 cis and trans-3-(3-?yridyl)methylchroman-4,6-diol·
To a 0° C. solution of 17.86 g (70.0 mmol) of the title product of the preceding Example in 150 ml tetrahydrofuran and 150 ml methanol was added 7.94 g (0.21 mol) of sodium borohydride in small portions to avoid excessive foaming. The reaction was stirred 18 hours at 0°-25° C. and then the solvents were removed by evaporation in vacuo. The residue was dissolved in 100 ml water and 100 ml 4N hydrochloric acid (cold) and stirred 20 minutes. The resultant solution was basified with solid sodium bicarbonate and the mixture multiply extracted with ethyl acetate. The combined extracts were dried over magnesium sulfate and evaporated to an oil (this material is a monohydrate). Hydration interfers with the next alkylation step, thus the hydrated water was replaced by ethanol via three azeotropic distillations on a rotating evaporator with 100 ml each of ethanol. The resultant oil was dried in vacuo to a foam which by ^H-NMR analysis is a 3:5 mixture of trans: cis isomers complexed with 0.3 mol of ethanol.
AP 0 0 0 0 8 3
MS (m/e) 257 (M ), 137, 120 and 101.
Ή-NMR(DMSO-dg)delta(ppm)
1.02 (t, J=7 Hz, CH3 of EtOH), 2.02 and 2.13 (m, CH) , 2.42 (m, lCH2Ar) , 2.72 (m, ICH-Ar), 3.40 (m, Ch\ of EtOH), 3.72 (m, lCHnO),
z. z,
3.84 (m, CK2O), 3.97 (m, 1CH2O), 4.17 (after D2O exchange, d, J=4 Hz, trans isomer CHOD), 4.22 (after □2O exchange, d, J=2 Hz, cis isomer CHOD), 4.22 (t, J=6 Hz, OH of EtOH), 5.33 (d, J=6 Hz, OH), 5.42 (d, J=6 Hz, OH), 6.55, 6.70, 7.28, 7.58, 7.67 and 8.28 (m, 7 ArK), 8.77 and 8.81 (s, OH).
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-30EXAMPLE 4A cis-3- ( 3-?yridyl) methylchroir.ar.-4, 6-diol
A mixture of the title product of Example 3 (6.0 g, 0.023 mol) and cerium, chloride heptahydrate (CeCl^ 7H2O? 5,25 9> 0.0141 mol) in methanol (125 ml) was cooled to 0-5° C. and sodium borohydride (0.445 g, 0.0117 mol) was added in three portions. The reaction was stirred at room temperature for 0.5 hours. Methanol was then removed in vacuo and the foamy residue was treated with saturated NH^Cl solution, followed by extraction with ethyl acetate. The organic layer was dried over MgSO^ and concentrated in vacuo to a foam.
The foam was treated with toluene and then pumped under ' '' high vacuum for several hours. This was repeated two more times to give present title product (5.7 g, 94%).
.iw j
-NMR analysis (see preceding Example) indicated about Ό 4% contamination with the trans-isomer.
EXAMPLE 5
Λ ~ cis and trans-3-(3-Pyridylmethy1-6s' 2θ (2-quinolyl) methoxv-4-chromano1
To a 0° C. solution of 18.3 g (71.2 mmol) of a 3:5 mixture of trans and cis-3-(3-pyridyl)methylchroman4,6-diol and 13.3 g (75.1 mmol) of 2-(chloromethyl)quinoline in 75 ml of dry dimethyIformamide was added 1.80 g (75.1 mmol·) of sodium hydride as a 60% mineral oil suspension. The reaction was stirred 1 hour at 0-20° C. followed by quenching with the addition of excess saturated ammonium chloride. The quenched reaction was extracted with ethyl acetate and the organic extract washed twice with saturated sodium chloride, dried over sodium sulfate and evaporated to an oil. This crude product was purified via column chromatography on 1 kg of silica gel eluted with 10% isopropanol/10% ethyl acetate/80% dichloromethane to give in order of elution
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-31the title cis-Isomer, 10.31 g (36%), ς>.ρ. 107-110° C., and crude trans-isomer which was rechromatographed on 750 g of silica gel to yield the title trans-isomer, 4.89 g (17%), m.p. 123-126° C. after recrystallization from methylene chloride/et’ner.
cis-isomer. MS (m/e) 398 (M+), 288, 261, 256,
IR (CHC13) 3591, 3285 (OH),
238 , 210 and 142 (100%) . 1617, 1601 and 1577 cm-1
Ή-NMR(CDCi.) delta(ppm) :
2.22 (m, C-3H), 2.59 (dd, 0 = 12, 6 Hz, lCH2Ar), 2.87 (dd, 0=12,
Hz, ICH^Ar) , 4.00 (m, OCH.J , 4.40 (d,
£.
0=3.37 Hz, CHOH), 5.22 (s, CH2O), 6.73 (d, 0 = 8 Hz, C-8H), 6.32 (d, 0 = 2 Hz, C-5H), 6.85 (dd, J=8, 2 Hz, C-7H), 7.18 (m, ArH), 7.48 (dd, 0=8, 3 Hz, ArH), 7.55 (m, 2ArH), 7.66 (dd, J=8, 8 Hz, ArH), 7.75 (d, J=8 Hz, ArH), 7.95 (d, J=8 Hz, ArH), 8.10 (d, J=8 Hz, ArH), 8.40 (m, ArH) and 8.47 (m, ArH).
Analysis calculated for C^H-^h^O^
C, 75.36? H, 5.56; N, 7.03%.
Found:
C, 75.15; H, 5
5; N, 6.89% .
.+ ,
AP 0 0 0 0 8 3 trans-isomer. MS (m/e) 398 (M ) , 288 , 261 , 256 and 142 (100%). IR (CKCl.*) 3583, 3302 (OH), 1618, 1601 and 1577 cm
Ή-NMR(CDC13)delta(ppm): 2.15 (m,
C-3K) , 2.48 (dd, J=13, 3 h’z, lCH2Ar) , 2.69 (dd, J=13, 6 Hz, lCH2Ar), 3.85 (dd, J=12, 6 Hz, 1CH2C), 4.15 (dd, J=12, 3 Hz, 1CH2O), 4.41 (d, J=3.95 Hz, CHOH), 5.27 (s, CK?O), 6.77 (d, 0=8 Hz, C-8H), 6.90 (dd, 0=8, 2Hz,
C-7H), 7.16 (m, ArH), 7.46 (m, 2ArH), 7.63 (d, J=8 Hz, ArH), 7.69 (m, ArH), 7.79 (d, 0=8 Hz, ArH), 8.02 (d,
J=8 Hz, ArH), 8.15 (d, J=S Hz, ArH), 8.36 (m, 2ArH). Analysis calculated for ^25^22^2^31
C, 75.36; H, 5.56; N, 7.03%.
Found: C, 75.15; H, 5.55; N, 6.89%.
bad or»g'nal
-32O
Ο
Ο .0
EXAMPLE 5A cls-3-(3-Pyridyl)methy1-6-(2quinolv1) me thoxy-4-chromanol
To a solution of 10 g (38.8 mmol) of the title 5 product of Example 4A and 7.02 g (39.5 mmol) of
2-chloromethylquinoline in 70 ml dimethylformamide was added, in one portion, 1.58 g (39.5 mmol) of sodium hydride (60% dispersion in mineral oil). The reaction was stirred for 2 hours at 25° C., quenched with an excess of saturated ammonium chloride, and extracted with ethyl acetate. The organic phase was washed with water and saturated sodium chloride, dried over magnesium sulfate, and evaporated to a foam, which was crystallized and recrystallized from chloroform-diiso15 propyl ether to yield 11.1 g (72%) of present title product, identical with cis-product of the preceding Example .
EXAMPLE 6
3S,4S- and 3R,4R-3-(3-?yridyl)raethyl-6-(220 quinolyl)methoxy-4-chromany1 R-O-Acetylmandelate
To a 0° C. solution of 4.00 g (10.1 mmol) of the title cis-isomer of the preceding two Examples, 2.30 g (11.8 mmol) of (R)-(-)-O-acetylmandelic acid and 1.44 g (11.8 mmol) of 4-N,N~dimethylaminopyridine in 20 ml dichloromethane was added 2.27 g (11.0 mmol) dicyclohexylcarbodiimide. The reaction mixture was stirred 16 hours while warming to 25° C. The reaction was filtered and the filtrate evaporated to an oil. Column chromatography of this crude product on 600 g silica gel eluted with 3% isopropanol-5% ethyl acetate-92% dichloromethane gave in order of elution 1.78 g (31%) of 3S^,4S-title diastereoir.er and 2.08 g (36%) of 3R,4R-title diastereomer as oils.
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-333S,4S-iscmer: MS (m/e) 574 (M+) , 397, 381, 288, 238, 149, 147 and 142 (100%). IRtCKCl^) 1745 (C=O), 1619, 1600 and 1573 cm-1.
1H-NMR(CDC13)delta(ppm); 1.91 (dd, J=15, 10 Kz, lCH2Ar), 2.2 (lCH2Ar overlap with 2.23), 2.23 (s, Ac),
2.35 (m, C-3H) , 3.87 (m, OCHp , 5.29 (s, CH2O) , 5.93 (d, J=3 Hz, C-4H), 5.98 (s, mandelate CH), 6.76 (d, J=9Hz, C-8H), 6.96 (m, C-5, 7H) and 7.1-8.5 (9m, 15 ArH) .
3R,4R-isomer: MS (m/e) 574 (;·Γ) , 397 , 381 , 288 (100%), 261 , 238 , 147 and 142. I.R(CHC13) 1742 (q=O) ,
1619, 1601 and 1577 cm-1.
1H-NMR(CDC13)delta(ppm): 2.22 (s, Ac), 2.48 (m, C-3H),
2.57 (dd, J=14, 9 Hz, lCH2Ar), 2.83 (dd, J=14, 6 Hz, lCH2Ar), 3.98 (m, OCHJ , 5.08 (m, CH2O), 5.99 (s, mandelate CH), 5.93 (d, J=3 Kz, C-4H), 6.59 (s, J=3 Hz, C-5H), 6.69 (d, J=9 Hz, C-8H), 6.84 (dd, J=9, 3 Hz, C-7H) and 7.1-8.5 (8m, 15 ArH).
The structure and absolute stereochemistry of these isomers was proven by X-ray crystallographic analyses. For this purpose, the 3S,4S-isomer was recrystallized from methanol, m.p. 135-136° C.
[alpha]^ = -7.78° {tetrahydrofuran, c - 0.0465).
Analysis calculated for Co_HonN_0,:
J j J U 4. Ό
C, 73.15; H, 5.26; N, 4.83%.
Found: C, 72.83; H, 4.89; N, 5.00%
For the same purpose, the 3R,4R-iscmer was recrystallized from CKC1,/hexane, m.p. 126.5-123° C.
J [alpha]^ = +50.65° (tetrahydrcfuran, c = 0.034).
Analvsis calculated for C^cH-,nNnOr· iH-.O:
□5 30 2 6 2
C, 72.59; H, 5.31; N, 4.84%.
Found: C, 72.39; H, 5.30; N, 4.80%
AP 0 0 0 0 8 3
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-34EXAMPLE
3S-(3-Pyridyl) methy1-6-(2quinolyi)methoxy-4S-chromanol
The 3S,4S-title diastereomeric ester of the preceding Example, 1.73 g (3.10 mmol) and 4.92 g (35.7 mmol) of potassium carbonate in a mixture of 38 ml methanol, ml tetrahvdrofuran and 10 ml water was stirred 16 hours a.t 25° C. The organic solvent was removed on a rotating evaporator and the residue dissolved in 500 ml water and 150 dichloromethane. The organic layer along with three 100 ml extracts of the aqueous layer was dried over magnesium sulfate and evaporated to an oil. This crude product was crystallized from diiscpropyl ether/dichloromethane to yield 1.06 g (88%) of 20 the title compound, m.p
-98.40
137-138° C., [alpha]
Ο
142 .
-1 cm 1 (CH3OH, ¢=0.01045).
MS (m/e) 398 (M+ 100%), 288, 263, 256, 238 and IR (CHC13) 3589, 3244 (OH), 1618, 1600 and 1577
K-NMR(CDC13)delta(ppm):
2.24 (m, C-3H), 2.16 (dd,
Hz , lCH2Ar) ,
4.41 (d, J=3 Hz, C-4H), 5.22 (s, CHnO),
J=14, 8 Hz, lCH2Ar), 2.89 (dd, J-14,
4.02 (m, OCH-),
6.75 (d, J=8 Hz, C-8H), 6.83 (d, J=2 Hz, C-5H), 6.86 (dd, J=8, 2 Hz, C-7H), 7.2 (m, lArH), 7.49 (m, lArH), 7.57 (m, 2ArH), 6.67 (add, J=8, 8, 2 Hz, lArH), 7.77 (d, J=8 Hz, lArH), 7.98 (d, J=S Hz, lArH), 8.11 (d, J=8 Hz, lArH), 8.42 (m, lArH) and 8.49 (d, J=2 Hz, lArH). Analysis calculated :or C25K22N2°3:
Found:
C, 75.36; H, 5.56; N, 7.03%. C, 75.06; H, 5.36; N, 7.00%.
bad ORIGINAL g
-35EXAMPLE 8
3R-(3-Pyridyl) methy1-6-(2cuinoiyi)methoxy-4R-chrcmanol
By the procedure of the preceding Example, the 5 title 3R,4R-diastereomeric ester of Example 6, 2.08 g (3.62 mmol) was converted to present title product,
1.15 g (80%), crystallized from diisopropyl ether/di20 chloromethane, m.p. 137-138° C., (alphal^ « +98.40° (CH-jOH, c=0.00985) .
MS (m/e) 398 (M+), 288, 261, 256, 238 and 142 (100%). IR(CKC13) 3588, 3285 (OH), 1619, 1600 and 1577 cm1. 1H-NMR(CDC13)delta(ppm): 2.24 (m, C-3H),
2.61 (dd, J = 14, 8, lCH2Ar), 2.89 (dd, J=14, 8 Hz, lCH2Ar), 4.02 (m, OCH2), 4.41 (d, J-3 Hz, C-4H), 5.22 (s, CH2O), 6.75 (d, J=8 Hz, C-8K), 6.83 (d, J=2 Hz,
C-5H), 6.86 (dd, J=8, 2 Hz, C-7H) , 7.2 (m, 1 ArH), 7.49 (m, lArH), 7.57 (m, 2 ArH), 6.67 (ddd, J=8, 8, 2 Hz, lArH), 7.77 (d, J=8 Hz, lArH), 7.98 (d, J=8 Hz, lArH), 8.11 (d, J = 8 Hz, lArH), 8.42 (m, lArH) and 8.49 (d, J=2
Hz , lArH) .
Analysis calculated for C?c.H22N2O3:
C, 75.36; H, 5.56; N, 7.03%.
Found: C, 75.19; H, 5.38; N, 6.97%.
EXAMPLE 9 *
5 6-Benzyloxv-3-phenoxv-4-chromanone
A solution of 17 g of 3-diazo-6-benzyloxy-4chromanone and 17 g of phenol in 100 ml of toluene was heated to 110° C. in an oil bath. Rhodium (II) acetate dimer (50 mg) was added in one portion. After nitrogen evolution ceased (5 minutes), the reaction was allowed to cool to room temperature, diluted with ethyl acetate and washed with 10% sodium hydroxide to remove excess phenol. The organic layer was dried over sodium sulfate and evaporated in vacuo to give the crude product,
AP 0 0 0 0 8 3
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-36.'X’
Ο which was purified by column chromatography on silica gel eluting with dichloromethane to give 2.6 g of product m.p. 100-102° C.
1H-NMR(CDC13) delta(ppm) : 4.4 (m, 2H) , 4.84 (m, IK) ,
4.88 (s, 2H) , 6.82-7.40 (m, 13H) .
EXAMPLE 10
6-Hydroxy-3-phenoxy-4-chromanone A mixture of 2.76 g of the title product of the preceding Example, 60 mi of ethyl acetate and 850 mg of
10% Pd/C catalyst was hydrogenated at 44 psig for 4 hours. The catalyst was removed by filtration and the filtrate evaporated in vacuo to give title product as a vellow solid, m.D. 142-146° C.
MS (m/e) calculated for C]_5H]_2°4 : 256.0736;
found: 256.0713.
^H-NMR(acetone-dg) delta(ppm) : 4.6 (m, 2H) , 4.15 (dd,
1H) , 6.8-7.3 (m, 8H) .
EXAMPLE 11 cis- and trans-3-Phenoxychroman-4,6-diol
To a solution of 1.86 g of the title product of the preceding Example in 50 ml of tetrahydrofuran was added 550 mg of lithium aluminum hydride. The reaction was stirred for 2 hours, then quenched with water, acidified to pH 4 with dilute hydrochloric acid and extracted with ethyl acetate. The ethyl acetate layer was dried ever sodium sulfate and evaporated in vacuo to give the crude product mixture which was triturated with CH2Cl2 and filtered to yield pure cis-title product, 650 mg, m.p. 207-208° C. The filtrate was evaporated in vacuo and separated by column chromatography on silica gel eluting with chloroform/ether. A total of 800 mg of less polar cis and 450 mg of more polar trans product (m.p. 144-146° C) were obtained.
BAD ORIGINAL Γ
-2>Ίcis-isomer: MS (m/e) 25S (M+-) .
*H-NMR(acetone-dg)delta(ppm): 4.00-4.13' (m, 2H) , 4.75 (m, 1H), 4.95 (m, 1H) , 6.5-7.40 (m, 8H).
EXAMPLE 12 cis-3-Phenoxychroman-4,6-diol
A mixture of 10.04 g of the title product of Example 9, 200 ml of methanol, 100 ml of tetrahydrofuran and 1 g 10% Pd/C catalyst was hydrogenated at 44 psig for 24 hours. The catalyst was recovered by filtration and the filtrate was evaporated in vacuo to obtain the crude product, which was triturated with dichloromethane and filtered to give 4.9 g of title product having properties identical to those of the cis-title product of the preceding Example.
EXAMPLE 13 ( + ) -cis-3-Phenoxy-6-(2-quinolyl)methoxy-4-chromanol £ 8 0 0 0 0 dV
To a solution of 800 mg of the title product of the preceding Example and 835 mg of 2-chloromethylquinoline in 55 ml of dimethylformamide was added 299 mg of 50% NaH. The reaction was allowed to stir at room temperature for 3 hours, then poured into water and extracted with ethyl acetate. The ethyl acetate layer was dried over sodium sulfate and evaporated in vacuo to give the crude product, which was purified by trituration with ether to yield 455 mg of title product, m.p. 151-153° C.
1H-NMR(DMSO-dg)delta(ppm) : 1H), 4.85 (s, 1H), 5.30 (s, 6.65-8.0 (m, 14H), 8.40 (d,
4.1-4.3 (m, 2H), 4.75 (s 2H), 5.55 (d, J=l, 1H), J=1, 1H).
BAD ORIGINAL
-33EXAMPLE 14 { + ) -trans-3- Phe.noxy-6- ( 2-quinolyl) methcxy-4-chromanol
To a solution of 450 mg of the trans-title product of Example 11 and 461 mg of 2-chloromethylquinoline in
30 ml of dimethylformamide was added 168 mg of 50% sodium hydride. The reaction was allowed to stir at room temperature for 3 hours, then poured into water and extracted with ethyl acetate. The ethyl acetate layer was dried over sodium sulfate and evaporated in vacuo to give the crude product, which was purified by column chromatography on silica gel eluting with dichloromethane and recrystallization from CH2Cl2/isopropyl ether to give 160 mg of title product, m.p. 127° C.
EXAMPLE 15 (+)-cis-6-(5-Fluoro-2-benzothiazolyl)methoxy-3-phenoxy-4-chromanol·
A mixture of 256 mg of cis-title product of Example 11, 221 mg of 2-chloromethv1-6-fluorobenzothiazole, 415 mg of potassium carbonate, 165 mg of sodium iodide and 25 ml of acetone was heated at reflux overnight. The reaction was cooled to room temperature and the inorganics were removed by filtration. The filtrate was evaporated to afford the crude product, which was purified by column chromatography on silica gel eluting with CH2Cl2/ether and trituration with ether to give 150 mg of product, m.p. 144-145° C.
MS calculated for C^H^NES: 423.0940; found 423.0914.
XK-NMR(acetone-dg) delta (ppm) : 4.2-4.45 (m, 2H),'4.85 (s, 1H) , 5.05 (s, 1H) , 5.5 (s, 2H) , 6.7-7.4 (m, 7H) ,
7.75 (d, J=2, 1H), 3.05 (m, 1H) .
BAD ORIGINAL &
-39EXAMRLS 16
3S,4R- and 3R,4S-3-Phenoxy-6-(2-quinolylime thoxy- 4 -chromanyl R-O-Acetylmandelate
To a solution of 1.96 g of the title product of 5 Example 13, 710 mg of dimethylaminopyridine and 1.13 g of (R)- (-)-O-acetylmandelic acid in 125 ml of dichloromethane was added 1.2 g of dicyclohexylcarbodiimide.
The reaction was allowed to stir at room temperature overnight. The precipitated dicyclohexyl urea was removed by filtration and the filtrate evaporated in vacuo to afford the product mixture. It was separated by column chromatography on silica gel eluting with dichloromethane/isopropyl ether. The less polar product was collected and recrystallized from ethyl acetate/hexane to give 610 mg product, m.p. 92-94° C. The more polar product was collected and recrystallized from ether/hexa.ne to give 577 mg of product, m.p. 107-108° C.
One of these diasterecmeric cis-compounds possesses title 3S,4R-chromanyl stereochemistry and the other
3R,4S-chromanyl stereochemistry. Although their absolute stereochemistry has not yet been independently determined, based upon its polarity and optical rotation, it is believed that the less polar isomer is the 3S,4R-diastereoisomer.
ad η η η o ft 3
BAD ORIGINAL ft
-40EXAMPLE 17 (-)-3S -Phenoxy-6-(2-quinolv1) ♦ methoxy-4R -chromanol
A mixture of 610 mg of the less polar ester of the 5 preceding Example, 1.7 g of potassium carbonate, 4 ml water, 13 ml methanol and 13 ml of tetrahydrofuran was stirred at room temperature overnight. Excess potassium carbonate was removed by filtration and the filtrate was evaporated in vacuo to afford the crude product, which was purified by dissolution in ethyl acetate and washing with water. The ethyl acetate layer was dried and evaporated to give 400 mg of title product, m.p. 155-157° C. [alphajp = -21.6° (c = .005, tetrahydrofuran) .
The absolute stereochemistry of this cis-product is believed to be 3j3,4R.
EXAMPLE 18 .
* (+)-3R -Phenoxy-6-{2-quinolyl)* methoxy-4S -chromanol
A mixture of 577 mg of the more polar mandelate ester of Example 16, 1.6 g of potassium carbonate, 4 ml of water, 13 ml of tetrahydrofuran and 13 ml of methanol was stirred at room temperature overnight. Excess potassium carbonate was removed by filtration and the filtrate evaporated in vacuo. The residue was dissolved in ethyl acetate and washed with water. The ethyl acetate layer was dried and evaporated to give 300 mg of product, m.p. 159-160° C. [alpha]., = +19.6° (c = .005, tetrahydrofuran). The absolute stereochem30 istrv of this cis-oroduct is believed to be 3R,4S.
BAD ORIGINAL £
-41EXAMPLE 19
6-Benzylcxy-3- (4-methoxyphenoxy) -‘i-chromar.or.e
Replacing the phenol with a molar equivalent of 4-methoxyphenol, the method of Example 9 was employed to convert 3-diazo-6-benzyloxy-4-chromanone (22 g) to present title product, 6.8 g, m.p. 98-100° C.
EXAMPLE 20 (+)-cis-3-(4-Methoxyphenoxy)-4,6-chromandiol
By the method of Example 12, the product of the preceding Example (6.8 g) was converted to present title product, 2.7 g, m.p. 137-189° C.
(DMSO-d6) delta (ppm) : 3.70 (s, 3H) , 4.05-4.30 (m,
2H), 4.55 (s, 1H), 4.80 (s, 1H), 6.50-7.10 (m, 7H) .
EXAMPLE 21 (+)-cis-3-(4-Methoxyphenoxy)-6(2-quinoly 1) methoxy-.4-chromanol
By the method of Example 13/ the product of the preceding Example (2.7 g) was converted to present title product 1.1 g, m.p. 131-132° C.
1H-NMR(DMSO-dg) delta(ppm) : 3.65 (s, 3H) , 4.05-4.3 (m,
2H) , 4.55 (s, 1H) , 4.85 (s, 1H) , 5.30 (s, 2H) , 5.55 (d, J=l, 1H), 6.70-9.4 (m, 13H).
EXAMPLE 22 ( + )-cis-6-(5-Fluoro-2-benzothiazoly1) 25 methoxy-3-(4-methoxyphenoxy)-4-chromanol
By the method of Example 15, title product of
Example 20 (0.70 g) was converted to present title product, 0.11 g, m.p. 180-181° C.
MS (m/e) calculated for c24H20NO5FS: 453.1047; found:
453.1043.
AP 0 0 0 0 8 3
BAD ORIGINAL
Γ'*Τ
42EXAMPLE 23 ( + )- and (-)-cis-3-(4-Methoxyphenoxy) 6-(2-quinolyl)methoxy-4-chromanol
By the methods of Examples 16, 17 and 18, the title product of Example 21 (3.55 g) was resolved into title products:
(+)-isomer, 0.29 g, m.p. 152-154° C, [alpha]D = +40.0° believed to be the 3S,4R-isomer.
(c = 0.005, CH2C12) (-)-isomer , 0.40 g, (c = 0.005, CH9C12)
m.p. 152-154° C, [alpha)D = -42.9* ; believed to be the 3R, 4S_-isomer.
EXAMPLE 24
6-Benzyloxy-3-(3-methoxyphenoxy)-4-chromanone
Replacing the phenol with a molar equivalent of 3-methoxyphenol, the method of Example 9 was employed to convert 3-diazo-6-benzyloxy-4-chromanone (76 g) to present title product, 5.5 g, m.p. 98-100° C. 1H-NMR(CDC13)delta(ppm): 3.85 (s, 3H), 4.45-4.65 (m,
2H), 5.0-5.2 (m, 3H) , 6.5-7.6 (m, 12H).
EXAMPLE 25 &
ϊ>
(+)-cis-3-(3-Methoxyphenoxy)-4,6-chromandiol
3y the method of Example 12, the product of the preceding Example (5.5 g) was converted to present title product, 2.3 g, m.p. 187-189° C.
MS (m/e) calculated for 288.0998;
found: 288.0989.
EXAMPLE 26 ( + )-cis-3- (3-Methoxyphenoxy)-6(2-quinolvl)methoxy-4-chromanol
By the method of Example 13, the product of the preceding Example (2.26 g) was converted to present title product, 3.5 g, m.p. 128-129° C.
BAD ORIGINAL &
-43XAMPLE 27
7-3enzyloxy-3,4-dihydro-4-phenoxy1-benzoxenin-5(2H)-one
To a solution of 1.4 g of phenol in 50 ml o tetrahydrofuran was added 720 mg of 50% sodium hydride. After stirring for 30 minutes, a solution of 4.5 g of crude 7-benzyloxy-4-bromo-3,4-dihydro-l-benzoxepin5(2H)-one was added. The reaction was allowed to stir at room temperature for 5 hours. The tetrahydrofuran was evaporated in vacuo, and the residue dissolved in ethyl acetate and washed with water. The ethyl acetate layer was dried over sodium sulfate and evaporated in vacuo to give the crude product which was purified by recrystallization from methanol to give 2 g of title product, m.p. 112-114° C.
MS (m/e) calculated for C28H20°4: 360.1361;
found: 360.1401.
EXAMPLE 28
3,4-Dihydro-7-hvdroxy-4-phenoxyl-benzoxeoin-5(2H)-one
AP 0 0 0 0 8 3
A mixture of 2 g of the product of the preceding Example, 200 mg of 10% ?d/C and 50 ml of methanol was hydrogenated in a Parr shaker at 50 psig for 2.5 hours. The catalyst was removed by filtration and the filtrate evaporated in vacuo to give 1.5 g crude product which was used without purification in the next step.
BAD ORIGINAL
-44EXAMPLE 29 cis and trar.s-2,3,4,5-Tetrahydro4-phenoxy-l-benzoxepin-5,7-diol
To a solution of 3.5 g of the product of the 5 preceding Example in 100 ml of tetrahydrofuran was added 1 g of lithium aluminum hydride. The reaction was allowed to stir at room temperature for 15 minutes, then quenched with water, acidified to pH 4 with dilute hydrochloric acid and extracted with ethyl acetate.
The ethyl acetate layer was dried over sodium sulfate and evaporated in vacuo to give the product mixture.
It was separated by column chromatography on silica cel eluting with dichloromethane/ether, yielding 0.9 g of less polar trans-title product and 1.2 g of more polar cis-title product, both as oils.
EXAMPLE 30 (+)-trans-2,3,4,5,-Tetrahydro-4-phenoxy-7(2-quinolyl)methoxy-l-benzoxepin-5-ol
To a solution of 840 mg of the trans-title product of the preceding Example in 25 ml of dimethy1fcrmamide was added 154 mg of 50% NaH. After stirring for 20 minutes, 570 mg of 2-chloromethyIquinoline was added.
The reaction was stirred at room temperature for 1 hour, poured into water and extracted with ethyl acetate.
The ethyl acetate layer was dried over sodium sulfate and evaporated in vacuo to afford the crude product.
It was purified by recrystallization from dichloromethane/ isopropyl ether to give 820 mg of product, m.p. 128-129° C.
bad original
V
-45EXAMPLE 31 ( + ) -cis-2 ,3,4,5,-Tetrahydro-4-p'nenoxv-772-quinolyl)methoxy-l-benzoxepin-S-ol ·
To a solution of 950 mg of the cis-title product 5 of Example 29 in 25 ml of dimethylformamide was added
173 mg of 50% sodium hydride. After stirring for 20 minutes, 641 mg of 2-chloromethylquinoline was added. The reaction was stirred at room temperature for 1 hour, poured into water and extracted with ethyl acetate. The ethyl acetate layer was dried over sodium sulfate and evaporated in vacuo to afford the crude product which was purified by recrystallization from methyl acetate/hexane to afford 200 mg of title product, m. p.- 130-132° C.
EXAMPLE 32
7-Benzyloxy-2,3-dihydro-4-(3-pyridyloxy) l-benzoxepin-5(2H)-one
By the method of Example 27, substituting the phenol with a molar equivalent of 3-hydroxypvridine,
7-benzyloxy-4-bromo-3,4-dihydro-l-benzoxepin-5(2H)-one (4.1 g) was converted to present title product, 2.6 g, m.p. 140-141° C.
MS (m/e) calculated for C^^H.QNO.: 361.1392;
z2 19 4 found: 361.1396.
EXAMPLE 33
2,3-Dihydro-7-hydroxy-4-(3-pyridyloxy) l-benzoxepin-5(2H)-one
By the method of Example 28, the title product of the preceding Example (3.3 g) was converted to present title product, 2.5 g, m.p. 182.-.185? C.·
APO 0 0 0 8 3
BAD ORIGINAL £
-46EXAMPLE 34 cis- and trans-2,3,4,5-Tetrahvdro-4(3-pyridyioxy)-l-benzoxepin-5, 7-diol
By the method of Example 29, the product of the 5 preceding Example (2.5 g) was converted to present title products.
cis-isomer, 1.1 g, more polar.
^H-NMR(acetone-dg)delta(ppm) : 2.2-2.4 (m, 2H),
3.90-4.15 (m, 2H) , 4.90 (t, J=4, 1H), 5 .20 (s, 1H),
10 6.70-8.35 (m, 7H) .
trans-isom er , 0.84 g, m.p. 154-155° C., less polar.
.‘Ο
Ο
Ό
JO
EXAMPLE 35 ( + )-trans-2,3,4,5-Tetrahydro-4-(3-pyridyloxy) 7-(2-quinolyl)methoxy-l-benzoxepin-5-ol
By the method of Example 30, the trans-title product of the preceding Example (0.80 g) was converted to present title product, 0.23 g, m.p. 134-136° C.
MS (m/e) calculated for C25H22^2°4: 414.1579;
found: 414.1635.
EXAMPLE 36 (+)-cis-2,3,4,5-Tetrahydro-4-(3-pyridyloxy)7-(2-cuinolvl)methoxy-l-benzoxepin-5-ol
By the method of Example 31, the cis-title product of Example 34 (1.1 c) was converted to present title product, 0.35 g.
XH-NMR (DMSO-dg) delta (ppm) : 2.40 (m, 2n) , 3.80 (m, 1H) ,
4.10 (m, 1H), 4.85 (s, 1H), 5.0 (d, 1H), 5.35 (s, 2H), 5.80 (d, 1H), 6.9-8.5 (m, 13H).
BAD OR'G'nAL
-47EXAMPLE 37
7-Benzyloxy-3,4-dihydro-4-(3-(methoxycarbonyl) phenoxy)-l-benzoxepin-5(2H)-one
By the method of Example 27, substituting the 5 phenol with a molar equivalent of methyl 3-hydroxybenzoate, 7-benzyloxy-4-bromo-3,4-dihydro-1-benzoxepin5(2H)-one (17.3 g) was converted to present title
product, 10.9 g, m.p. 134 -136° C.
MS (m/e) calculated for C 22H19 NO4: 361. . 1392;
found: 361.1396.
1H-NMR(CDC13)delta(ppm): 2.40 (m, 1H) , 2.95 (m, 1H),
3.95 (s, 3H), 4.0 (m, 1H) , 4.6 (m, 1H) , 5.05 (s, 2H),
5.30 (t, J=4, IK), 6.9-7. 7 (m, 12H)
EXAMPLE 38
3,4-Dihydro-7-hydroxy-4-(3-(methoxycarbonvl)phenoxy)-l-benzoxepin-5(2H)-one
By the method of Example 28, the title product of the preceding Example (10.9 g) was converted to present title product, 6.3 g.
EXAMPLE 39 cis- and trans-2,3,4,5-Tetrahydro-4-(3-methoxycarbonyl)phenoxy)-l-benzoxepin-5,7-diol
To a solution of 4.7 g of the title product of the preceding Example in 100 ml of methanol was added
550 mg of sodium borohydride. The reaction was stirred at room temperature for 1 hour, then quenched with water. The solvent was evaporated in vacuo and the residue dissolved in ethyl acetate and washed with water. The ethyl acetate layer was dried over sodium sulfate and evaporated in vacuo to afford the crude product mixture. It was separated by column chromatography on silica gel, eluting with dichloromethane/ether, yielding 1.14 g of the less polar trans-title product and 1.3 g of the more polar cis-title product.
APO00083
BAD ORIGINAL
-48cis - isomer
1H) (s ,
2.1 (m,
H-NMR(CDC1^)deIta(ppm):
3.8 (s, 3H), 3.3-4.1 (m, 2H), 4.61 1H) , 6.5-7.6 (m, 7H) .
1H-NMR(CDC13)delta(ppm): 2.1 (m,
3.7 (t. 3H). 3.85 (s. 3H1. 4.15 fm.
, 2.4 (m, 1H) ,
1H), 4.90 (s, trans -isomer 5 1H), 2.3 (m, IK),
1H), 4.35 (m, 1H), 4.90 (m, 1H), 6.6-7.6 (m, 7H). EXAMPLE 40 (+)-trans-2,3,4,5-Tetrahydro-4-(3-methoxycarbonyl)phenoxv-7- ( 2-quinolyl) methoxy) -l-benzoxepin-5-ol
By the method of Example 30, the title product of the preceding Example (1.1 g) was converted to present title product, 1.3 c, tic (ether) Rf 0.65.
EXAMPLE 41 ( + ) -cis-2,3,4,5-Tetrahvdro-4-(3-methoxycarbonyl)15 phenoxv-7-(2-quinolyl) methoxy-1-benzoxepin-5-ol
By the method of Example 31, the cis-title product of Example 39 (1.3 g) was converted to present title product, 0.6 g.
1H-NMR(DMSO-d ) delta(ppm) : 2.25 (m, 2K), 3.80 (s, 3H) ,
3.85 (m, 1H) , 4.05 (m, 1H) , 4.85 (m, 1H) , 5.1 (s, 1H) ,
5.3 (s, 2H), 6.85-8.3 (m, 13K).
EXAMPLE 42 ( + )-cis-2,3,4,5-Tetrahydro-4-(3-carboxyphenoxy) 7-(2-cuinolyl) methoxy-1-benzoxepin-5-ol
To a solution of 630 mg of the title product of the preceding Example in 100 ml methanol and 25 ml tetrahydrofuran was added 10 ml of 5N NaOH. The reaction was heated on a steam bath for 10 minutes.
The volatiles were evaporated in vacuo, and the residue dissolved in water and acidified to pH 5 with dilute HCl. The precipitated product was collected by filtration and allowed to air-dry, 0.21 g, m.p.
110-116° C. (dec.).
MS (m/e) calculated for C-,-,Η-,-,ΝΌ , : 457.1525;
z / z j o found; 457.1541.
BAD ORIGINAL
-49EXAMPLE 43 ( + )-trans-2,3,4,5-Tetrahvdro-4-(3-carboxvphenoxy)7-(2-quinolyL) me thoxy-l-benzoxepin-5-ol
To a solution of 1.3 g of the title product of
Example 40 in 100 ml methanol and 25 ml tetrahydrofuran was added 10 ml of 5N NaOH. The reaction was heated on a steam bath for 10 minutes. The volatiles were evaporated in vacuo and the residue dissolved in water and acidified to pH 5. The precipitated title product was collected by filtration and purified by trituration with isopropyl ether, 0.13 g, m.p. 186-13(8° C.
MS (m/e) calculated for Co_H23N0g.· 457.1525;
found : 457.1576 .
EXAMPLE 44
6-Benzyloxy-3-(3-(methoxycarbonyl)benzylidene)- 4-chromanone
A mixture of 17 g of 6-benzyloxy-4-chromanone,
11.3 g of 3-carbomethoxybenzaldehyde, 14.4 g of pyrrolidine, 100 ml of tetrahydrofuran and 300 ml of methanol was stirred at room temperature overnight.
The volatiles were evaporated in vacuo to afford the crude product, which was purified by column chromatography on silica gel eluting with dichloromethane. The product fractions were combined and concentrated to an oil which crystallized upon trituration with methanol to give 17.2 g of title product, m.p. 109-112° C.
AD ft ft ft ft ft 3
BAD ORIGINAL
-50EXAMPLE 45
6-Hvdroxy-3- ( 3- (methoxycarbonyl) benzyl) -4-chromanone
A mixture of 17 g of the product of the preceding Example, 1.7 g of 10% Pd/C catalyst, 200 ml of tetra5 hydrofuran and 200 ml of methanol was hydrogenated in a Parr shaker at 40 psig for 3 hours. The catalyst was removed by filtration and the volatiles were evaporated in vacuo to give 10.6 g of title product.
^H-NMR(acetcne-dg)delta (ppm) : 2.65-3.30 (m, 3H) , 3.80 (s, 3H) , 4.2 (dd, J=4, J=3, 2H), 6.80-3.30 (m, 7K) .
EXAMPLE 46 cis- and trans-3-(3- (Methoxycarbonyl·)benzyl) chroman-4,6-diol
Φ By the methods of Example 39, the product of the
15 preceding Example was converted to present chromatographed title products in about the same yields. This cis-isomer, m.p. 135-137° C., is less polar and the trans-isomer, m.p. 158-160° C., is more polar; tic (7:3
CH2C12:ether) Rf 0.25 and 0.20, respectively.
EXAMPLE 47 ( + ) -cis-3-(3-(Methoxycarbonyl)benzyl)6-(2-quinolyl)methoxy-4-chromanol
By the method of Example 30, the cis-title product of the preceding Example was converted to present title product in about the same yield.
EXAMPLE 48 ( + ) -trans-3-(3-(Methoxycarbonyl)benzyl)6-(2-quinolyl)methoxy-4-chromanol
By the method of Example 30, the trans-title product of Example 46 was converted to present title product in about the same yield, m.p. 153-154° C.
MS (m/e) calculated for 455.1733;
found: 455.1721.
BAD ORIGINAL
-51EXAHPLE 49 (j) —cis—3— ( 3-Carboxybenzyl) -6(2-quinolyl)methoxy-4-chromano!
By the method of Example 42, the title product of 5 Example 47 (0.50 g) was converted to present title product, 0.14 g, m.p. 165-168* C.
EXAMPLE 50 (+)-cis-6-(5-Fluoro-2-benzothiazoly1)methoxy3- (3 - (me thoxy car bony 1) be.nzv 1) -4-chromano 1
By the method of Example 15, the cis-title product of Example 46 (0.74 g) was converted to present title product, 0.7C g, m.p. 171-173° C.
EXAMPLE 51 (+)-cis-3-(3-Carboxybenzyl)-6-{5-fluoro15 2-benzothiazolv1)methoxy-4-chromanol
By the method of Example 42, the title product of the preceding Example (0.70 g) was converted to present title product, 0.40 g, m.p. 208-210° C.
EXAMPLE 52 (+)-trans-3-(3-Carboxybenzyl)-6(2-quinolyl)methoxy-4-chromanol
By the method of Example 43, the title product of Example 43 (0.5 g) was converted to present title product, 0.1 g, m.p. 206-209° C.
EXAMPLE 53 ( + ) -trans-6- (5-Fluoro-2-ber.zothiazolyl) methoxy3-(3-(methoxycarbony1) benzyl)-4-chromanol
By the method of Example ±5, the trans-title product of Example 46 was converted to present title product in about the same yield.
EXAMPLE 54 (+)-trans-β-(5-Fluoro-2-benzothiazolyl)methoxv-3-(3-(carboxybenzyl-4-chromanol
By the method of Example 43, the title product of 35 the preceding Example (0.45 g) was converted to present title product, 0.33 g, m.p. 189-190° C.
ΔΡ Ω 0 0 0 8 3
BAD ORIGINAL $
4-chromanone ηαΔ
EXAMPLE 55 6 - (2-Qu ino1y1) metho xyA mixture of 6-hydroxy-4-chrcmanone (10.0 g,
0.0609 mol), 2-chloromethyiquinoline'(11.9 g, 0.0670 5 mol), sodium iodide (10.0 g, 0.0670 mol), potassium carbonate (25.3 g, 0.183 mol), and acetone (200 ml) was refluxed overnight under N2 atmosphere. After 17 hours the reaction appeared lighter and tic analysis (10% EtOAc/CH^Cl,) indicated complete conversion of starting 10 material to a slightly less polar product. The mixture was cooled, filtered, and the filtrate concentrated in vacuo. The residue was taken up in ethyl acetate (400 ml), washed with and brine, dried over MgSO^, and concentrated in vacuo to a dark brown oil. Purification 15 on a silica gel column eluted with 10% ethyl acetate/CH^Cl^ gave title product as an off-white solid, 15.3 g (82%), m.p. 112-114° C.; tic (1:9 ethyl acetate:CH2C12)
Rf 0.30.
EXAMPLE 56
3-[4-(Methoxycarbony1)benzylidene]-6(2-quinolyl)methoxy-4-chromanone
By the method of Example 1, the product of the preceding Example (5.0 g, 0.0164 mol), methyl 4-formylbenzoate (3.2 g, 0.0194 mol) and pyrrolidine (9.37 ml,
0.0286 mol) were converted to present title product,
5.36 g, tic (isopropyl ether) Rf 0.25.
BAD ORIGINAL Ά
-53EXAMPLE 57
3-(4-(Methoxycarbonyl) benzyl]-6(2-quinolyl)methoxy-4-chromanone
Title product of the preceding Example (3.6 g) in 5 50 ml of tetrahydrofuran was hydrogenated on a Parr shaker at 45 psig over 0.40 g of 10% Pd/C for 3 hours. The catalyst was recovered by filtration over diatomaceous earth, and the filtrate stripped to yield crude title product (2.9 g) which was purified by trituration with ether, 1.38 g.
MS (m/e) calculated for C23H23NO5: 453.1596 ;
found : 453 . 1552.
EXAMPLE 58 cis and trans-3-(4-(Methoxycarbonyl)benzy1]15 _6-(2-quinolyl)methoxy-4-chromanol_
By the method of Example 4, except to use methanol alone as solvent and 10:1 Cl^C^iethyl acetate as chromatography eluant, the product of the preceding Example (1.3 g) was converted to 1.4 g of crude mixture, separated into 0.14 g of less polar, cis-title product, m.p. 138-140° C. and 0.13 g of more polar, trans-title product, m.p. 152-154° C.
MS (m/e) calculated for C2gH,3NO3: 455.1733; found : 4 55 . 1695 .
EXAMPLE 59 (+)-trans-3-(4-Carboxybenzv1)-6(2-quinolyl)methoxy-4-chromanol
By the method of Example 43, the trans-title product cf the preceding Example (0.24 g) was converted to present title product, 0.1 g, m.p. 214-215° C.
bad original
-54Q ί»
EXAMPLE 60 ( + )-cis-3- ( 4-Carboxybenzyl )-6(2-quinolyl)methoxy-4-chromanol
By the method of Example 42, the cis-title product 5 of Example 58 (0.19 g) was converted to present title product, 0.06 g, m.p. 199-201° C.
MS (m/e) calculated for C27H23NC>5: 441.1576;
found: 441.1578.
EXAMPLE 61
3-Hvdroxymethy Iene-6 - (2-quinolyl)methoxy-4-chromanene
To a solution of title product of Example 55 (7.00 g, 0.0229 mol) and excess ethyl formate (35 mi) in toluene (80 ml) at room temperature under argon was added in portions over 5 minutes 2.2 g (0.0458 mol) of
50¾ sodium hydride in mineral oil. The yellow-green mixture was stirred at room temperature for 5 minutes, followed by the addition of 2 drops of ethanol to initiate the reaction. Within 5 minutes the mixture turned red-orange with gas evolution and was mildly exothermic. The mixture was stirred at room temperature for 1 hour, after which tic (5% CK3OH/CH2C12) indicated complete conversion of starting material to a more polar product. The reaction mixture was* poured into 400 ml of ice water, adjusted to pH 5 with 2N HC1, and extracted with ethyl acetate (500 ml). The organic laver was washed with H~0 and brine, dried over MgSO,, * Z 4 and concentrated in vacuo to a pasty yellow solid. Repeated trituration with hexanes to remove mineral oil gave present title product in 85% yield, tic (1:19
CH3O'n’:CH2Cl2) Rf 0.40.
BAD ORIGINAL 0
-55EXAMPLE 62
3-Diazo-6- (2-quinolyl) methoxy-4-chromanone To a solution of the title product of the preceding Example (7.60 g, 0.023 mol) and dry triethylamine 5 (6.4 ml, 0.046 mol) in dry CH2C12 (100 ml) at -30° C.
(dry ice-acetone bath) was added dropwise over 20 minutes a solution of tosyl azide (4.5 g, 0.023 mol) in CH2C12 (25 ml). The reaction mixture was allowed to gradually warm to room temperature overnight with stir10 ring. After 13 hours tic (20% ethyl acetate/CH2Cl2) indicated complete disappearance of starting material and formation of a less polar product. The mixture was treated with IN NaCH (100 ml) and stirred for 10 minutes. After treating with brine, the layers were separated and the organic layer was diluted with 200 ml of ethyl acetate. Methylene chloride was then removed in vacuo. The ethyl acetate residue was washed with H2O and brine, dried over MgSO^, and concentrated in vacuo to give present title product as a dark yellow solid, 6 g (90%); tic (1:.4 ethyl acetate:CH2<3i2)
Rf 0.27.
EXAMPLE 63
3-?henyithio-6-(2-quinolyl)methoxy-4-chromanone
To a suspension of title product of the preceding
Example (3.0 g, 0.0091 mol) and thiophenol (3.0 ml,
0.029 mol) in 15 mi of dry toluene at 70° C. was added 4 mg of rhodium II acetate dimer. Immediately the reaction became homogeneous, turning dark, with gas evolution. Tic analysis . (20% ethyl acetate/CH2Ci2) indicated complete conversion of starting material to a major less polar product. The reaction was cooled, diluted with ethyl acetate (50 ml), washed with IN NaOH (3x50 ml), H2<0 and brine, dried over MgSO^, and
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-So10 concentrated 1n vacuo to a dark brown oil. Purification by silica gel column chromatography eluting with 10% ethyl acetate/CH2Cl2 gave title product, 1.83 g (49%), tic (1:4 ethyl ace tate: CH2Cl2) Rf 0.50.
EXAMPLE 64
3- ( 2-Pyridylthio) -6- (2-quinolyl)methoxy-4-chromanone
By the method of the preceding Example usj.ng 15% ethyl acetate/CH2Cl2 as chromatography eluant, title product of Example 62 (2.00 g) and 2-mercaptopyridine were converted to present title product, 1.13 a (45%); tic (1:4 ethyl acetate :CH_C1^) Rf 0.57 .
EXAMPLE 65
3-Benzyloxy-6- ( 2-quinolyl) methoxy-4-chromanone
DS
JO
By the methods of Example 63, title product of Example 62 (2.00 g) and benzyl alcohol were converted to present title product, 0.94 g (38%); m.p.
113-115° C., tic (1:4 ethyl acetate:CH2C12) Rf 0.69. EXAMPLE 66 ( + ) -cis and tra.us-3-Phenylthio-67 2-quinolyl)methoxy-4-chromanol
To a suspension of the title product of Example 63 (1.85 g, 0.00447 moi) in 70 ml of methanol at 0-5° C. was added in portions 203 mg (0.00538 mol) of sodium borohydride. The reaction was warmed with stirring to room temperature, then diluted with 20 ml of tetrahydrofuran to obtain a homogeneous mixture, which, after stirring for 1.2 hours was concentrated in vacuo and the residue taken up in 400 ml ethyl acetate, washed with K-0 and brine, dried over Xa.SO,, and concentrated in vacuo to a yellow-white foam. Purification was carried out by silica gel column chromatography eluting with 20% ethyl acetate/CH2Cl2. The less polar cis-title product was isolated as a white solid (1.11 g, 60%). Recrystallization from isopropanol/hexanes gave 1.04 g
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-3/10 of white crystals, m.p. 144-146° C. The more polar trans-title product was obtained as a mixture, contaminated with a small amount of the less polar product. It was re-chromatographed using the same eluant to yield 400 mg (22%) of trans-title product as a white foamy solid. Recrystallization from toluene-hexanes afforded 280 mg of white crystals, m.p. 100-102° C.
cis-isomer. IR(KBr) 750, 1015, 1210, 1500 cm MS (m/e) 415.1191. Analysis calculated for C23H21NO3S C, 72.27; H, 5.09; N, 3.37%.
C, 72.78; H. 5.42; N, 3.36%.
IR(KBr) 750, 1015, 1215, 1500 cm Analysis calculated as for C, 72.10; H, 5.02; N, 3.30%. EXAMPLE 67
Found :
trans-isomer .
MS (m/e) 415.1311. cis-isomer. Found (+)-cis- and trans-3-Pyridylthio-6(2-quinolyl)methoxy-4-chromanol
By the method of the preceding Example, the title product of Example 64 (1.11 g) was converted to present title products, using 40% ethyl acetate/CH2Cl2 as eluant in the chromatographic separation.
cis-isomer, 0.50 g (45%), m.p. 136-138° C.
IR(K3r) 1210, 1500 cm-1. MS (m/e) 416.1239. Analysis calculated foi
C, 69.21; H, 4.84; N, 6.73%.
Found: C, 68.83; H. 4.86; N, 6.47%.
C24H20N2°3S: trans-isomer, 0.44 g (39%), m.p. 52-55° IR(KBr) 1500 cm \ Analysis calculated as for cis-isomer .
Found: C, 68.33; H, 4.82; N, 6.45%.
C.
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-58EXAMPLE 63 (Ζ ~cιs-3- (3enzy loxy )-6-( 2-quinoly 1) methoxv-4-chromanol
By the method of Example 66, the title product of Example 65, 0.70 g, was converted to present title product, in this instance without isolation of the corresponding trans-isomer. Chromatography on silica gel using 30% ethyl acetate/C^Cl^ as eluant gave purified title product, 0.53 g (75%), m.p. 134-136° C. IR(KBr) 1495 cm-1. MS (m/e) 413.1579 (M+) . Analysis calculated for
C, 75.53 ; H, 5.61; N, 3.39%.
Found: C, 75.29; H, 5.62; N, 3.34%.
EXAMPLE 69
L.» ο
ΠΟΑ (+)-cis-3-(Phenylsulfinyl)-6(2-quinolyl) methoxy-4-chromanol
To a solution of the cis-title product of
Example 66 (240 mg, 0.57 mmol) in CH^Cl^ (25 ml) at
0° C. was added 125 mg (0.57 mmol) of m-chloroperbenzoic acid. The reaction was stirred at 0° C. for 2 hours, after which tic (5% MeOH/CH^Cl^) indicated complete conversion of starting material to a less polar product.
The reaction was diluted with (50-70 ml), washed with saturated NaHCO-, H-0 and brine, dried over Na-SO., 3 2 2 4 and concentrated in vacuo to an off-white foamy solid. Purification by silica gel column chromatography eluting with 5% MeOH/C^C^ the sulfoxide title product as a white solid, 240 mg, 96%. Recrystallization from hexane-toluene afforded a white crystalline product, m.p. 174-176° C. IR (KBr) 1500 cm x. Analysis calculated for C~-H-,, NO.S : C, 69.59; H, 4.91; N, 3.25%.
u o u x 4
Found: C, 69.90; K, 4.93; N, 3.21%.
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-59EXAMPLS 70 ( + )- trans-3- (Phenylsulfinyl)-6( 2-quinoIvl) methoxy-4-chromanol
To a room temperature solution of the trans-title 5 product of Example 66 (230 mg, 0.55 mmol) in methanol (30 ml) was added a solution of KHSO.J (oxone monopersulfate; 340 mg, 0.55 mmol) in (10 ml). After 0.5 hours, the reaction mixture was diluted with ethyl acetate (300 ml) and H2<0 (300 ml). The organic layer was separated, washed with (2x300 ml) and brine (300 ml), dried over Na2SO^, and concentrated ir. vacuo to a foamy solid. Purification by silica gel column chromatography eluting with 5% MeOH/Ci^C^ afforded the sulfoxide title product as a foamy solid (185 mg, 77%).
Recrystallization from isopropanol/hexane gave 170 mg of white solid, m.p. 169-171° C. IR (KBr) 1490 cm-^.
EXAMPLE 71 (+)-cis-3-(Benzenesulfonyl)-6(2-quinolyl)methoxy-4-chromanol
To a partial solution of the cis-title product of
Example 66 (500 mg, 1.20 mmol) in 50 ml of hot methanol was added a solution of K'HSO^ (2.20 g, 3.58 mmol) in Η2θ (20 ml). Upon adding the oxone solution, the reaction turned milky with a white precipitate forming.
The reaction was stirred at room temperature. After 5 minutes, tic (20% ethyl acetate/CH2Cl2) indicated complete conversion of starting material to 2 more polar products in an approximate ratio of 1:1, presumably sulfoxide and sulfone. The reaction was stirred for an additional 25 minutes and then diluted with h’2O (200 ml) and ethyl acetate (250 ml). The organic layer, which still contained a significant amount of undissolved solid, was washed 2xH2O and brine, and filtered to give 170 mg of white solid. The filtrate was then dried
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-60over Na SO^ and concentrated in vacuo to give an oil which slowly solidified. Purification by silica gel column chromatography, eluting with 20% ethyl acetate/CH2Cl2 afforded 300 mg (56%) of sulfone title product as a white solid. Recrystallization from isopropanol/hexane gave 280 mg of white solid, m.p. 168-171° C. IR (KBr) 1500 cm MS (m/e) calculated: 447.1140, found: 447.1149.
EXAMPLE 72 o-Methoxy-3-(3-pvridyloxy)-4-chrcmanone
To a room temperature solution of 3-hydroxvpyridine (7.55 g, 0.0778 mol) in dimethylformamide (400 ml) was added in portions 3.74 g (0.0780 mol) of 50% sodium
:.0 hydride. The mixture was stirred at room temperature
L 15 for 30 minutes, followed by the addition of 20.0 g (0.0778 mol) of 3-bromo-6-methoxy-4-chromanone all at once. The resulting red-orange mixture was stirred at
Q room temperature for 1 hour, after which ~tlc (20% ethyl > acetate/CH2Cl-,) indicated complete conversion of starting material to the more polar products. The reaction mixture was poured into 1.2 liter of h'2O, adjusted to pH 3-9 with IN NaOH and extracted with ethyl acetate (2x800 ml). The combined extracts were washed with K2O and brine, dried over Na^O^, and con25 centrated in vacuo to a yellow oil. Purification by flash column chromatography using 2.3 Kg of fine mesh silica gel and eluting with 20% ethyl acetate/CH2Cl2 afforded title product as a pale yellow solid in 3.6% yield, m.p. 135-136° C.
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-61EX .AMPLE 7 3
6-Hydroxy-3- (3-pyridyloxv) -4-chromanone To a solution cf the title product of the preceding
Example (1.60 g, 5.90 mmol) in 150 ml of dry CI^C^ at 5 -78° C. was added slowly via syringe 11.S ml (11.8 mmol) of 1M boron tribromide over 30 minutes. The resulting red mixture was allowed to gradually warm to -20° C. with stirring and was then placed in the freezer (-10° C.) overnight. The next day the reaction mixture was stirred at 0-5° C. (ice bath) for 1 hour followed by the addition of 150 ml of I^O. The mixture was stirred at 0-5° C. for 2 hours more and then the layers were separated. The organic phase was extracted with 150 ml of H^O and the combined aqueous extracts were adjusted to pH 8 with IN NaOH and extracted with ethyl acetate (2x; total of 600 ml) . The combined extracts were washed with brine, dried over Na^SO^, and concentrated in vacuo to a yellow solid. The crude product was chromatographed on a silica gel flash column, elut20 ing with 5% CH^OH/CH^Cl., to afford title product as a yellow solid, 640 mg (42%), tic (1:19 CH^OK: C^Cl^)
Rf 0.21.
EXAMPLE 74 cis-6-Kydroxy-3- (3-pyridyloxy) -4-chromanol
To a solution of the title product of the preceding Example (640 mg, 2.49 mmol) in 2:1 CH^OK: tetrahydrofuran (45 ml) at 0-5° C. was added 96 mg (2.49 mmol) cf sodium borohydride. The yellow solution was stirred at room temperature and turned almost colorless within 5 minutes at which time tic (10% MeOH/CH-Cl-,) indicated comDlete conversion of starting material to the more polar product. Approximately 20 mg more NaBH^ was added, the mixture stirred for 10 minutes, and then concentrated in vacuo.
ad ft ft η n ft 3
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-62The crude mixture was purified by flash chromatography on a short silica column, eluting with 10% CH-OH/CH 0C1 to afford present title product as a white solid,
610 mg (94%), m.p. 194-197° C.
EXAMPLE 75 (+)-cis-6-(6-Fluoro-2-quinolyl)methoxy3-(3-pyridyloxy)-4-chromanol
To a room temperature solution of the title product of the preceding Example (450 mg, 1.74 mmol) and 6-fluoro-2-chloromethylquinoline (374 mg,
1.91 mmol) in dimethylformamide (17 ml) was added in portions 92 mg (1.91 mmol) of 50% sodium hydride in oil. The mixture was stirred at room temperature, the color gradually changing from light yellow to brown.
After 1 hour, tic (10% CH3OK/CH2CI2) indicated formation of a major less polar product and only a trace of starting material. The reaction mixture was poured into 200 ml of H2O and extracted with ethyl aceb-ate (2x200 ml). The combined extracts were washed with H2O and brine, dried over Na2SO^, and concentrated in vacuo to a dark yellow oil. Purification by silica gel flash column chromatography, eluting with 5% CH^OH/C’r^C^, afforded title product as an off-white solid, 460 mg (63%). Recrystallization from isopropyl ether-CH0Cl? gave 420 mg of white crystalline solid, m.p.
157-159° C.
IR (K3r) 1240, 1480, 1495 cm“x. MS (m/e)
418.1302 . Analysis calculated for :
C, 68.89; H, 4.58; N, 6.69%.
Found: C, 69.20; H, 4.41; N, 6.62%.
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-63EXAMPLE 76 (+)-cis-3-(3-Pyridyloxy)-6( 2-quinolyl) methoxy-4-chromanol
By the methods of the preceding Example, substitut5 ing a molar equivalent of 2-chloromethylquinoline for the 6-fluoro-2-chloromethylquinoline, the title product of Example 74 (0.26 g) was converted to present chromatographed title product, 0.35 g (88%), also recrystallized from isopropyl ether/C^C^, m.p. 126-128° C.
IR (XBr) 1500 cm1. MS (m/e) 400.1433. Analysis calculated for C_.H„nN_O , :
z 0 2 4
C, 71.99; K, 5.03; N, 7.00%.
Found: C, 71.52; H, 4.90; N, 6.86%.
EXAMPLE 77 i + )-cis-6-(5-Fluoro-2-benzothiazolyl) methoxv-3-(3-pyridyloxy)-4-chromanol
By the method of Example 75, substituting a molar equivalent of 2-chloromethyl-5-fluorobenzothiazole for the 6-fluoro-2-chloromethylquinoline, the title product of Example 74 (0.40 g) was converted to present chromatographed title product; 0.19 g (29%), also recrystallized from isopropyl ether/CH^C^, m.p. 213-215° C.
IR (XBr) 1260, 1495 cm1. MS (m/e) 424.0870.
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EXAMPLE 73 (^)—cis—6—(2-?yridyl)methoxy-3(3-pyridyloxy)-4-chromancl
By the method of Example 75, substituting a molar equivalent of 2-picoIyl chloride for the 6-fluoro-2chlorometnylquinoline and replacing the chromatography eluant with ethyl acetate, title product of Example 74 (0.246 g) was converted to present chromatographed title product, 0.19 g (57%), also recrystallized from isopropyl ether/CH?Cln, m.p. 148-150° C.
IR (KBr) 1270, 1435, 1500 1590 cm Analysis calculated for ^θΚ^Ν,,Ο^
C, 68.56; H, 5.IS; N, 8.00%.
Found: C, 68.19; H, 4.77; N, 7.81%.
EXAMPLE 79 (+)-cis-6-(3-Pyridy1)methoxy-3(3-pyridyloxy)-4-chromanol
By the method of Example 75, substituting a molar equivalent of 3-picolyl chloride for the 6-fluoro-2chloromethyleuinoline and using 10% methanol/ethyl acetate as chromatography eluant, title product of Example n4 (0.259 g) was converted to present chromatographed title product, 0.23 g (66%), recrystallized in the manner of the preceding Example, m.p. 139-141° C.
IR (KBr) 1425, 1500, 1575 cm x. MS (m/e) 350.1272 (M+) .
EXAMPLE SO ( + )-cis-6-(4-Pyridyl) methoxy-3By the method of Example 75, substituting a molar equivalent cf 4-picolyl chloride for the 6-f^oro-2chloromethyleuinoline and using the same chromatography eluant as in the preceding Example, the title product of Example 74 (0.259 g) was converted to present
BAl) ORIGINAL
-6 5chromatographed title product, 0.23 g (66%), recrystallized in the manner of Example 73, m.p. 149-151° C.
IR (X3r) 1260, 12S0, 1490, 1575 cm'1. Analysis calculated for C^gH^gN^O^
C, 68.56; H, 5.18; N, 8.00%.
Found: C, 63.12; H, 5.12; N, 7.78%.
EXAMPLE 81
6-Methoxy-3-(2-thiazolyl)methylene-4-chromanone
By the method of Example 1, 6-methoxy-4-chromanone (3.97 g, 0.0223 mol) and 2-thiazolecarbaidehyde (3.00 g, 0.0265 mol) were converted to present title product which was recrystallized from methanol, 1.20 g (20%), m.p. 130-132° C., tic (1:19 CH-jOH :CH2C12) Rf 0.67.
EXAMPLE 82
3-(3-1ndolvl)methylene-6-methoxy-4-chrcmanone
By the method of Example 1, 6-methoxy-4-chromanone (10.0 g) and 3-indolecarbaldehyde were converted to present title product recrystallized from CH^OH/Cl^C^, 16.7 g (98%), m.p. 217-219° C.
EXAMPLE 83
6-Methoxy-3- ( 2-thiazolyl) methyl-4-chromanor.e
By the method of Example 2, the title product of Example 81 (1.20 g, 0.0044 mol) was converted to present title product recrystallized from hexanes,
1.01 g (83%), m.p. 71-73° C., tic (1:1 ethyl acetate:CH2Cl2) Rf 0.39.
EXAMPLE 84
3-(3-Indolyl)methvl-6-methoxy-4-chromanone
By the method of Example 2, the title product of Example 82 (15.5 g) was converted to present title product, recrystallized from isopropanol/hexanes,
12.5 g (80%), m.p. 121-123° C.
ΑΡ0 0008 3 /ftWi
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EXAMPLE 85
6-Hydroxy-3- ( 2-thiazolyl) methyl-4-chromanone
By the method of Example 3, the title product of Example 83 (2.10 g) was converted to present title product recrystallized from isopropyl ether/hexanes,
1.80 g (95%), m.p. 150-152° C., tic (1:19 CH-jOH:CH2C12) Rf 0.23.
EXAMPLE 86
6-Hydroxy-3-(3-indolyl)methyl-4-chromanone 10 By the method of Example 73, the title product of
Example 84 (2.50 g) was converted to present title product, 0.43 g (1S%), m.p. 188-190° C.
EXAMPLE 87 cis- and trans-3- [ (2-Thiazolvl)15 _methyl) chroman-4,6-diol_
By the method of Example 4, the title product of Example 85 (1.74 g, was converted to a crude product, purified but not separated by column flash chromatography on silica gel, eluting with 1:9 CH^OH:CH2C12, to yield present title products as a mixture, 1.73 g (98¾), m.p. 50° C.
EXAMPLE 33 cis- and trans-3-[ (3-Indolyl)methyl]chroman-4,6-diol
By the method of Example 4, the title product of
Example 86 (0.40 g) was converted to a mixture of present title products, 0.40 g, m.p. 78° C. (dec.).
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EXAMPLE 39 ( + )-cis- and trans-6- ( 2-Quinolyl)methoxy_3-(2-thiazolyl)methyl-4-chromanol_
By the method of Example 5, using 1:39 CH^OK^H^Cl·^ as eluant in the chromatographic separation of the cis-trans isomers, and recrystallizing the final products from isopropyl ether-hexane, the title product mixture of Example 87 was converted to less polar cis-title product, 0.28 g (40%), m.p. 50° C. (dec.), and more polar trans-title product, 0.27 g (39%:
125-127° C.
cis-isomer. IR(KBr) 1210, 1495 cm
m. o.
-i trans-isomer. IR(X3r) 1225 , 1495 3280 cm Analysis calculated for ^23Η20Ν2θ3δ:
C, 68.30; K, 4.98; N, 6.93%.
Found: C, 68.19; H, 4.75; N, 6.60%.
EXAMPLE 90 ( + )-cis- and trans-6- (6-Fluoro-2-quinoly1) methoxy-3-(2-thiazolyl)methyl-4-chromanol
By the method of Example 5, using the-same eluant as the preceding Example for' initial chromatography, the mixed title products of Example 37 (0.45 g) and 2-chloromethyl-6-fluorocuinoline were converted to less polar cis-tirle product, triturated with hexanes,
0.18 g (24%), m.p. 47° C. (dec.) and crude, more polar trans-isomer. The latter was purified by column chromatography, using 30% hexane/ethyl acetate as eluant, thus yielding the trans-title product, 0.24 g (33%), recrystallized from isopropyl ether/C^Ci-,, m.p. 159-161° C.
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-68ί;cmer. IR(K3r) 1210, 15C0 cm \ MS (m/e)
422 . 1057. trans-i somer. IR(K3r) 1220, 1495 cm_i. MS
422 . 1103 . Analysis calculated for C?qFNpO^S:
c, 65.39; H, 4.53; N, 6.63%.
Found: C, 64.81; H, 4.00; N, 6.20%. . EXAMPLE 91
<v.
(+)-cis- and trans-6-(5-Fluoro-2-benzothiazolyl)methoxy-3-(2-thiazolyl)methv1-4-chromanol
3y the method of Example 5, the mixed title products of Example 87 (0.43 g) and 2-chioromethy15-fluorothiazole were converted to a crude mixture of title products. The trans-title isomer crystallized from the crude product with CH^CN, yield 0.08 g (12%) of this isomer, m.p. 168-170° C. The mother liquor was stripped and column chromatographed on silica gel using the eluant of Example 89 to yield the cis-isomer,
0.14 g, recrystallized from isopropyl ether/CK^Cl^, m.p. 138-140° C.
cis-isomer. IR(X3r) 1220 , 1500 cm MS (m/e)
428.0689. Analysis calculated for ^21^17^2^3^2:
C, 58.73; H, 4.22; N, 6.52%.
Found: C, 58.66 ; H, 4.03; N, 6.42%.
trans-isomer. IR(X3r) 1430 , 1460 1500 cm MS (m/e) 428.0689 . Analysis calculated as for cis-isomer.
Found: C, 53.71; H, 4.09; N, 6.50%.
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-69EXAMPLE 92 (+)-cis-3 - (3 -1ndoly1) me thy1 - 6 (2-cuir,o ly 1 ) methoxy-4-chromano!
By the method of Example 5, without isolation of 5 the corresponding trans-isomer in this instance, the mixed title products of Example 88 (0.38 g) and 2-chloromethylquinoline, using 40% ethyl acetate/hexane as eluant on chromatography, were converted to present less polar title product, 0.163 g (29%), recrystallizec from isopropyl ether/hexane, m.p. 60° C. MS (m/e)
436 . 1762 .
EXAMPLE 93 (+)-cis-6-(2-Pyridyl)methoxy-373-pyridyl)methvl-4-chromanol
By the method of Example 5, using 10% isopropanol/CK2Cl2 as chromatography eluant, but without isolation of the corresponding trans-isomer in this instance, the mixed title products of Example 4 (1.00 g) and 2-picolyl chloride were converted to present less polar title product in similar yield, recrystallized from isopropanol/hexane, m.p. 108-110° C.
IR(KBr) 1220, 1505 cm1. MS (m/e) 348.1500.
Analysis calculated for C21H20^3°3:
C, 72.40; H, 5.79; N, 8.04%.
Found: C, 72.30; H, 5.66; N, 7.87%.
EXAMPLE 94 ( + )-cis- and trans-6- (1-Metny1-2-benzimidazoly1)m,ethoxy-3- (3-pyridyl)methyl-4-chromanol
By the method of Example 5, using the chromatog30 raphy eluant of the preceding Example, the mixed title products of Example 4 (0.97 g) were converted to present less polar cis-title product, 0.5 g (30%), recrystallized from isopropanol/hexane, m.p. 151-153° C., and more polar trans-title product, 0.27 g (18%), also recrystallized from isopropanol/hexane, m.p. 181-183° C.
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-70ΓΜ
75»
Ο
X)
Ο cis-isc.-.er . IR(XBr) 12 15 , 1500 cm”1. MS (m/e)
401. 1717.
trans-isomer. IR(XBr) 1490 cm1. MS (m/e)
401.1721. Analysis calculated for C-.H_-.N-0,:
23 3 3
C, 71.80; H, 5.77; N, 10.47%.
Found: C, 70.63; H, 5.67; N, 10.14%.
EXAMPLE 95 cis-6-(3-Pyridyl)methoxy-3(3-ovridvl)methvl-4-chromanol -* — — —
By the method of Example 89, the product of
Example 4A (0.70 g) and 3-picolyl chloride were converted to present title product, 0.61 g (64%), m.p. 145-147° C.
IR(KBr) 1500 cm”1. MS (m/e) 348.1464 (M+) .
Analysis calculated for c?3H20^2°3:
C, 72.40; H, 5.79; N, 8.04%.
Found: C, 72.01; H, 5.77; N, 7.98%.
EXAMPLE 96 .
cis-6-(4-Pyridyl)methoxy-320 (3-pyridyl)methy1-4-chromanol
By the method of Example 89, the product of
Example 4A (0.70 g) and 4-picolyl chloride were converted to present title product, C.57 g (60%), m.p. 100-102° C.
IR(KBr) 1220 , 1500 cm”1. MS (m/e) 348.1474 (if).
Analysis calculated as for preceding Example.
Found: C, 72.19; H, 5.72; N, 7.81%.
EXAMPLE 97
7,3-Dihydrc-7-methy1-3-(2-cuinoiyl) 3 0 _methoxy-5 ( 6H)--quinolone_
3y the method of Example 55, 7,8-dihydro-3-hydrcxy7-methyl-5(6H)-quinolone and 2-chloromethylquinoline were converted to present title product in 67% yield, m.p. 141-144° C.
MS (m/e) calculated: 318.1365; found: 318.1325.
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-71EXAMPLE 93
7-Methy1-6(8H)- (3-pyridyl)methylene3-(2-ouinolyl)methoxy-5(7H) -quinolone
By the method of Example 1, the title product of 5 the preceding Example was converted to present title product in 54% yield.
MS (m/e) 407.2 <M+); tic (19:1 CH2C12: ethanol)
Rf. 0.12.
EXAMPLE 99 cis- and trar.s-7,8-Dihydro-7-methv 1-6- (3-pyridyl) methyl-3-(2-quinolyl)methoxy-5(6H)-cuinolone
By the method of Example 2, the title product of the preceding Example was converted to a mixture of present title products in 42% yield; MS (m/e) 409.1 (M+) ; tic (9:1 CH2C12: ethanol) Rf 0.5. It is believed that this product is a mixture of 6,7-cis and 6,7-trans-isomers, although the possibility that the product comprises substantially one or the other of these isomers is not excluded.
EXAMPLE 100
5,6,7,S-Tetrahydro-c-’ and t-7-methyl-c-6-(3pyridy1)methy1-3-(2-quinolyl)methoxy-r-5-quinolol and
5,6,7,8-Tetrahydro-c- and t-7-methyl-t.-6-( 325 pyridyl)metnvl-3- (2-quinolyl)methoxy-r-5-quinolol
3y the method of Example 4, except to use ethyl acetate as solvent, the product of the preceding Example was converted tc present chrcmatographically separated title products named according to the IUPAC
0 Ncmenc '..ature of Organic Chemistry, 1979 Ed., pp. 477-3. Each of these products is believed to be a mixture of two compounds, one having the 7-methyl group cis (c) relative(r) to the 5-hydroxy group and the other having the 7-methyl group trans (t) relative (.r) to the 5-hydroxy
BAD ORIGINAL
-72group. However, the possibility that each of these products comprises substantially one orethe other of these c-7 or t-7 isomers is not excluded.' r-5, c-6-isomer(s) . ., ,_n ,„,a — ~_: 47% yield, m.p. 179-181° C.;
less polar; MS (m/e) 411.3 (M ), exact mass calculated: 411.1945, found: 411.1941.
r-5, t-6-isomer(s) . χθ% yield, m>p< 190_i93o C.; more polar; MS (m/e) calculated; 411.1945, found:
Analysis calculated for C„,H„cN,0.,:
ώ 0 Z 0 J z
75.89; H, 6.12; N, 10.21%.
1 1 . 1896 .
C,
Found; C, 75.78; H, 6.22; N, 9.82% or pn·*, o
□
EXAMPLE 101
6(8H)-Hydroxymethylene-7-methyl-3-(2quinoly1)methoxy-5(7H)-quinolone By the method of Example 61, the title produci
Example 97 was converted to present title product in 99% yield; tic (19:1 CH2C12: ethanol).Rf 0.6.
EXAMPLE 102
6(8H) -Diazo-7-methy1-3-(2-quinolyl)methoxy-5(7H)-quinolone
By the method of Example 62, the title product of the preceding Example was converted to present title product in 99% yield; Rf 0.25.
:1c (19:1 CH2C12: ethanol)
EXAMPLE 103 cis- and trans-7,3-Dihydro-7-methyl-6-phenoxy3-(2-quinolyl) methoxy-5(6H)-quinolone (See Example 99 for comment re isomer composition.
By the method of Example 63, the title product cf the preceding Example and phenol were converted to a mixture of present title products in 50% yield; MS (m/e) 410.1 (M+) ; tic (19:1 CH2C12:ethanol) Rf 0.56.
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-73EXAMPLE 104
5,6,7,8-Tetrahydro-c- and t-7-methyl-c-6phenoxy-3-(2-quinolyl)methoxy-r-5-quinolol and
5,6,7,8-Tetrahydro-c- and t-7-methyl-t-6phenoxy-3-(2-quinolyl)methoxy-r-5-quinolol (See Example 100 for comments re nomenclature and isomer composition.)
By the method of Example 66, the title products of the preceding Example were converted to present, chromatographically separated title products.
r-5, c-6-isomer(s)
43% yield, m.p. 166-167° C.;
less polar. MS (m/e) calculated: 412.1787, found: 412.1829.
r-5, t-6-isomer(s)
6% yield, m.p. 129-132° C.;
more polar. MS (m/e) calculated: 412.1787, found: 412.1778.
EXAMPLE 105
6(8H)-Benzylidene-3-benzyloxy7-methyl-5(7H)-quinolone
By the method of Example 1, 3-benzyloxy-7,8-dihydro3-methyl-5(6H)-quinolone and benzaldehyde were converted to present title product in 95% yield; MS calculated: 355.1752, found: 355.1567.
EXAMPLE 106 cis- and trans-6-Benzy1-7,8-dihydro-3hydroxy-7-methvl-5(6H)-quinolone (See Example 99 for comment re isomer composition.)
By the method of Example 2, the title product of the preceding Example was converted to a mixture of present title products in 67% yield; MS (m/e) 267.1 (M+) ; tic (5:1 CF^C^xethyi acetate) Rf 0.08.
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EXAMPLE 107
5,6,7,8-Tetrahydro-6-benzyl7-me thy1-3,5-quinclined ioIs
3y the method of Example 4, the title products of the preceding Example were converted to present title products in 99% yield, understood to be a mixture of £-5,c-6,c-7; r-5,t-6,c-7; r-5,c-6,t-7; and r-5,t-6,t-7 geometric isomers of similar polarity; tic (9:1
CH-C1-. :CH,OH) Rf 0.3.
2 j
EXAMPLE 108 c-6-Benzyl-5,6,7,8-tetrahydro-c- and t-7-methy1-3- (2-quinolyl) methoxy-r-5-quinolol and t-6-3enzyl-5,6,7,8-tetrahydro-c- and t-7-methy 1-3- (2-quinolyl)methoxy-r-5-qui.nolol (See Example 100 for comments re nomenclature and isomer composition.)
By the method of Example 5, the product of the preceding Example was converted to present chromatographically separated citle products.
r-5, c-6-isomer(s) .o„ , - . ,, , — ' —_: 18% yield, m.p. 146.3147.5° C.; less polar. MS (m/e) calculated: 410.1994, found : 410.20 4 5 .
r-5, t-6-rsomer(s).. ig% yield, n>p. 151-151.5° c.; more polar. MS (m/e) calculated: 410.1994, found:
410.1998 .
EXAMPLE 109 ( + )-cis- and trans-3 - (3-Pyridyl)methvl6-(2-guinoxalinyl)methoxy-4-chromanol
3y the method of Example 5, using gradient elution with 39:1, 29:1 and finally 19:1 CH2Cl2:CHgOR in chromatography, the title products of Example 4 and 2-chloromethyIquinoxaline were converted to chromatograp’nically separated title products, the cis recrystallized from toluene and the trans recrystallized from CH2C1O.
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-75cis-isomer. 16% yield, m.p. 163.5-169.5° C.; less polar. MS (m/e) calculated: 399.1583, found:
399.1569.
trans-isomer. 3% yield, m.p. 141.5-143° C.; more polar. MS (m/e) calculated: 399.1583, found:
399.1571.
EXAMPLE 110 cis-3-(4-Methoxyphenoxy)-6(2-pyridy1)methoxy-4-chromanol
By the method of Example 13, using flash chromatography with 39:1 Cr^Cl2: isopropanol as eluant and recrystallization from isopropyl ether/C^Cl^ for purification of the product, the title product of Example 20 and 2-picolvl chloride were converted to present title product in 26% yield, m.p. 117-118° C.
MS calculated: 379.1420, found: 379.1412.
EXAMPLE 111 cis-6-(6-Fluoro-2-quinolyl)-3(4-methoxyphenoxy)-4-chromanol
By the method of Example 13, using flash chromatography with 35:1 : isopropanol as eluant and recrystal1ization from CH^Cl^ for purification of the product, ths title product of Example 20 (0.20 g, 0.69 mmol) and 6-5luoro-2-chlorcmethylquinoline were converted to present title product, 0.104 g (34%), m.p. 151-153.5° C. MS calculated: 447.1486, found: 447.1494.
EXAMPLE 112 cis-6-(6-Fluoro-2-quinolyl)-3(3-methoxyphenoxy)-4-chromanol
By the method of Example 13, using flash chromatography with 49:1 CH^C^: isopropanol as eluant and recrystallization as in the preceding Example, the title product of Example 25 (0.37 g, 0.00128 mol) and
2-chloromethy1-7-fluoroquinoline were converted to ftpn η ο o 8 3
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-76present title product, 0.39 g from the column, 0.113 g after recrystallization, m.p. 131-132° C. MS (m/e) calculated: 447.1486, found: 447.1497.
EXAMPLE 113 cis-3-(3-Methoxyphenoxy)-6(2-pyridyl)methoxy-4-chromanol
By the method of Example 13, using flash chromatography with 40:9:1 CH-^C^ : hexane: isopropanol as eluant and recrystallization from 1:1 toluene: hexane, the title product of Example 25 (0.37 g, 0.00128 mol) and
2-picolyl chloride were converted to present title product, 0.15 g (30%), m.p. 105-107° C. .MS (m/e) calculated: 379.1424, found: 379.1394.
EXAMPLE 114
Diasteromeric cis-6-(6-Fluoro-2-quinolyl)methoxy-3-(3-pyridyloxy)-4-chromanyl
N-(R-l-Naphthylethyl)carbamates
The title product of Example 75 (0.93 g, 0.0022 mol), toluene (30 ml) and R-(1-naphthylethyl) isocyanate (2.0 g, 0.01 mol) were combined in the order listed ar.d the mixture heated at reflux for 18 hours by which time tic (19:1 CH2C12:CHgOH) indicated at least 95% conversion to a less polar product. The reaction mixture was then cooled to room temperature, washed 3x10 ml H20 ar.d the Η2<3 layer back-washed 2x8 ml ethyl acetate. The organic layers were combined, dried over MgSO^ and stripped to a gum (2.83 g), which was flash chromatographed on silica gel, using 1:1 toluene: ethyl acetate as eluant, to yield a purified mixture of the title diastereoisomers, 1.2 g. The diastereoisomers were separated by HPLC on with 53:47 hexanes: ethyl acetate as eluant to yield less polar diasteroisomer, 0.52 g; a mixture of diastereoisomers. suitable for recycling,
0.23 g; and more polar diastereoisomer, 0.31 g.
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-77EXAMPLE 115 (-)-cls-6-(6-Fluoro-2-quinolyl)methoxy_3-(3-pyridyloxv)-4-chromanol_
A flame-dried flask under dry N2 was charged in sequence with the less polar diastereoisomer of the preceding Example (0.497 g, 0.81 mmol), benzene (30 ml), triethylamine (0.404 g, 0.56 ml, 0.004 mol) and HSiCl^ (0.542 g, 0.40 ml, 0.004 mol) with vigorous stirring. The mixture was stirred for 61 hours at room temperature by which time tic (19:1 iCH^OH) indicated complete conversion to a single, more polar product. The reaction mixture was quenched with 60 ml K2O, the pH adjusted to 7 with IN NaOH, and the resulting emulsion filtered over diatomaceous earth.
The filtrate phases were separated and the aqueous phase washed 2x30 ml ethyl acetate. The organic layers were combined, dried over MgSO^ and the dry solution combined with 3 cc silica gel and the mixture stripped to dryness. The dry silica gel/product mixture charge to a silica gel chromatography column which was eluted with 19:1 CH2Cl2: isopropanol· and the resulting chromatographed title product recrystallized from methanol,
0.16 g, m.p. 163-164° C., believed to be the 3S , 4R-isomer , [alpha]^1 = -57» [c = 1.3 CH2C12].
EXAMPLE 116 (+)-cis-6-(6-FluorO-2-quinolyl)methoxy3-(3-pyridyloxv)-4-chromanol
By the method of the preceding Example, the more polar diastereoisomer of Example 112 (0.30 g, 0.49 mmol) was converted to present title product, 1.0 c, m.p. 163.5-164.5° C., believed to be the 3R,4S-isomer, [alpha]^1 = +57» [c = 1.1 in CH2C12J.
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-73ο α
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EXAMPLE 117 cis-6-(6-Fluoro-2-quinolyl)methoxy3-(3-methcxyphenoxy)-4-chrcmanyl
N,N-Dimethylglycinate
Title product of Example 112 (0.10 g, 0.22 mmol) was dissolved in CH2C12 (3 ml). 4-(Dimethylamino)pyridine (0.043 g, 0.35 mmol), Ν,Ν-diraethylglycine hydrochloride (0.038 g, 0.26 mmol) and dicyclohexylcarbodiimide (0.050 g, 0.26 mmol) were then added in the listed sequence and the mixture was stirred for 18 hours at which time tic (1:1 toluene:ethyl acetate/1% triethylamine) indicated complete conversion to a single, more polar product. The reaction mixture was directly chromatographed on a silica gel column using 1:1 toluene : ethyl acetate/1% triethylamine as eluant to yield purified title product as a white solid, 0.10 g (86%). MS (m/e) 532.3 (M+) ; tic (1:1 toluene: ethyl acetate/1% triethylamine) Rf 0.32.
EXAMPLE 118 cis-6-(6-Fluoro-2-quinolyl)methoxy3-(3-methoxyphenoxy)-4-chrcmanyl
N, N-Dimethylglycinate Dihydrochloride To the product of the preceding Example (0.10 g,
0.19 mmol) dissolved in 5 ml absolute ethanol was added
0.475 ml (0.475 mmol) of IN HC1 and the mixture stirred for several minutes, then stripped to dryness,and restripped 3x5 ml absolute ethanol and finally 1x5 ml CH2C12 to yield title product as a white solid, 0.11 g (95.6%), m.p. 149-152° C.
0 EXAMPLE 119 cis-3-(3-Methoxvphenoxy)-6-(2-quinolvl)methoxy-4-chromany1 N,N-Dimethylglycinate
To a solution of the title product of Example 26 (0.493 g), 4-(dimethylamino)pyridine (0.226 g) and
N,N-dimethylglycine hydrochloride (0.193 g) in 30 ml of
BAD ORIGINAL
-79was aaaed a s olution of dicyclohexylcarbodiimide in 5 ml of Ch^C^· After stirring 18 hours, by-prcduct dicyclohexyl urea was recovered by filtration and the filtrate stripped to an oily solid which was chromato5 graphed on a silica gel column with ether as eluant to yield purified title product, 0.44 g.
1H-NMR (CDC13) delta (ppm) : 2.15 (s, 6H) , 3.15 (s, 2H) , . 3.80 (s, 3H), 4.30 (d, J=5Hz, 2H), 4.90 (m, 1H), 6.30 (d, J=3, 1H) , 6.30-8.30 (m, 13H) .
EXAMPLE 120 cis-3-(3-Methoxyphenoxy)-6-(2-quinolyl)methoxy4-chromanvl· N,N-Pimethylglvcinate Dihydrochloride
Title product of the preceding Example (0.44 g) was reacted by the method of Example 118, but the reaction mixture was simply stripped to dryness and title product crystallized from isopropanol, 0.39 g, decomposes on heating without melting.
EXAMPLE 121 cis-3-(4-Methoxyphenoxy)-6-(2-quinolyl)20 methoxy-4-chromanyl N,N-Dimethylglycinate
3y the method of Example 119, the title product of
Example 21 (0.40 g) was converted to present title product, 0.43 g, tic (9:1 ethyl acetate:CH3OH) Rf 0.5.
EXAMPLE 122 cis-3-(4-Methoxyphenoxy)-6-(2-quinolyl)methoxy4-chromanvl N,N-DimethyIglycinate Dihydrochloride
By the method of Example 120, the title product of the preceding Example (0.35 g) was converted to present title product, 0.31 g, decomposes on heating without melting.
ado no o 8 3
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-soEXAMPLE 12 3 c is-3-Phenoxy-6 - ( 2-cuinolyl)methoxy4-chromany1 N,N-Dimethylglycinate
By the method of Example 119, the title product of 5 Example 13 (0.40 g) was converted to present title product, 0.43 g.
1H-NMR(CDCl3)delta(ppm) : 2.20 (s, 6H) , 3.10 (s, 2H),
4.30 (d, J=5, 2H), 4.90 (s, 1H), 5.30 (s, 2H), 6.30 (d, J=3, 1H) , 6.80-8.30 (m, 14H).
EXAMPLE 124 cis-3-Phenoxy-6-(2-cuinolyl)methoxy-4-chromany1 N,Ν-Dimethylglycinate Dihydrochloride
By the method of Example 120, the title product of the preceding Example was converted to present title product, 0.40 g, m.p. 157° C. (dec.).
EXAMPLE 125
6-Benzyloxv-3- (4-metnoxy-3-(methoxycarbonyl)benzylidene)-4-chromanone
By the method of Example 44, 6-benzyloxy-4-chro20 manone (20.95 g) and 4-methoxy-3-methoxycarbonylbenzaldehyde were converted to present title product,
25.75 g (73%); tic (19:1 CH_Cln:ether) Rf 0.70.
EXAMPLE 126
6-Hydroxy-3-{4-methoxy-3-(methoxv2 5 carbonyl)benzyl)-4-chromanone
By the method of Example 45, the title product of the preceding Example (25.75 g) was converted to present title product, 13.2 g (64.5%); tic (ether)
Rf 0.40.
^H-NMR(CDC13) delta(ppm) : 2.60-3.20 (m, 3H) , 3.90 (s,
3H), 3.95 (s, 3H), 4.1 (d, J=3, 1H), 4.35 (d, J=3, IK), 6.30-7.70 (m, 6H).
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-31EXAMPLE 127 cis- and trans-3-(4-methoxy-3-(methoxycarbony 1 ) benzyl)-4,6-chremandio 1
By the method of Example 39, using 10% ether/CH^Cl^ as eluant for chromatographic separation, the title product of the product of the preceding Example (13.2 g) was converted to present less polar cis-title product, 6.2 g, tic (7:3 CH2C12: ether)
Rf 0.30, and more polar trans-title product, 3.94 g, tic (7:3 CHnCl0: ether, Rf 0.25.
EXAMPLE 128 cis-3-(4-Methoxy-3-(methoxycarbony1)benzyl)6-(2-quinolyl) methoxy-4-chromanol
3y the method of Example 30, the cis-title product of the preceding Example (1.0 g) and 2-chloromethylquinoline were converted to present title product, purified by column chromatography on silica gel using 5% ether/CH2Cl2 as eluant, 0.50 g.
1H-NMR(CDC13) delta(ppm) : 2.3-2.9 (m, 3H) , 2.90 (s,
3H), 2.95 (s, 3H), 4.0 (m, 2H), 4.4 (s, 1H), 5.25 (s,
2H), 6.70-8.20 (m, 12H).
EXAMPLE 129 cis-6-(5-Fluoro-2-benzothiazolyl)methoxy-3(4-methoxy-3-(methoxycarbonyl)benzyl) -4-chromanol
By the method of Example 15, the cis-title product of Example 127 (1.0 g) and 2-chloromethvl-5-fluorcbenzothiazole were converted to present title product, purified by chromatography as in the preceding Example, 0.84 g, m.p. 160-162° C.
* 1H-NMR(CDC13)delta(ppm): 2.3-2.9 (m, 3H), 2.90 (s,
3H), 2.95 (s, 3H), 4.0 (d, J=2, 2H), 4.40 (s, IK), 5.30 (s, 2H), 6.70-7.80 (m, 9H).
AP 0 0 0 0 8 3
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-32EXAMPLE 130 trans-3- (4-Methoxy-3- (methoxycar-bony 1) benzyl) -r3-( 2-quinolyl) me thoxy-4-chromanol
By the method of Example 30, the trans-title product of Example 127 (1.0 g) and 2-chloromethy1quinoline were converted to present title product, purified by chromatography as in Example 128, 0.88 g. 1H-NMR(CDCl^)delta(ppm) : 2.1-2.8 (at, 3H), 2.90 (s,
6H) , 4.10 (d, J=4, 1H) , 4.4 (s, 1H) , 5.25 (s, 2H) ,
6.70-8.20 (m, 12H).
EXAMPLE 131 trans-6- ( 5-Fluoro-2-benzothiazolvl) methoxy-3(4-methoxy-3- (methoxycarbonyl) benzyl) -4-chromanol
By the method of Example 15, the trans-title
1-5 product of Example 127 (0.60 g) and 2-chloromethy15-fluorobenzothiazole were converted to present title product, purified by chromatography according to Example 128, 0.43 g.
1H-NMR(CDC13) delta (ppm) : 2.1-2.8 (in, 3H) , 2.90 (s,
6H) , 4.15 (d, J=4, 1H) , 4.4 (s, 1H) , 5.30 (s, 2H) ,
6.70-7.80 (m, 9H).
EXAMPLE 132 cis-3- (3-Carboxy-4-methoxybenzyl) -6(2-quinolyl) methoxy-4-chromanol
By the method of Example 42, the title product of
Example 123 (0.33 g) was converted to present title product, 0.25 g, m.p. 167-169° C.
EXAMPLE 133 cis-3- (3-Carboxy-4-methoxybenzyl) -6- (5-f luoro3 0 _2-benzothiazolvl) me thoxy-4-chromanol_
By the method of Example 42, the title product of
Example 129 (0.83 g) was converted to present title product, 0.56 g, m.p. 197-198° C.
BAD ORIGINAL ft
-83EXAMPLE 134 trar.s-3- (3-Carboxy-4-methoxybenzy 1) 6- ( 2-cuinolyl) methoxy-4-chromanol
By the method of Example 42, the title product of 5 Example 130 (0.50 g) was converted to present title product, 0.35 g, m.p. 172-174° C.
EXAMPLE 135 trans-3-(3-Carboxy-4-methoxybenzyl)-6-(5fluoro-2-benzothiazolyl)methoxy-4-chromanol
By the method of Example 42, the title product of
Example 131 (0.42 g) was converted to present title product, 0.28 c, m.p. 149-151° C.
EXAMPLE 136
2-Benzy1-3,4-dihvdro-7-methoxy-l(2H)-naphthalenone
To a -78° C. solution of lithium diisopropyl amide (from 43.8 ml (0.312 mol) of diisopropyl amine in 280 ml tetrahydrofuran and 119 ml (0.298 mol) of 2.5 n-butyllithium) was slowly added a solution of 50 g (0.285 mol) of 7-methoxy-3,4-dihydro-1 (2H)-naphthaienone in 100 ml tetrahydrofuran. The resultant reaction mixture was stirred 10 minutes at -78° C. The cooling bath was charged to a 0° C. ice bath, followed immediately by the rapid addition of 38 ml (0.32 mol) of benzyl bromide. HexamethyIphosphoramide (106 ml, 0.60 mol) was’then added and the resultant solution stirred at 25° C. for 2 hours. The reaction was added to saturated N’H^Ci and saturated NaCl, dried over MgSO^, and evaporated to an oil, which was purified via column chromatography on 1 Kg of silica gel eluted with 10% ether-hexane to give 20 g (26%) of the title compound as an cil.
>·,
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-8410 'X>
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MS (m/e) 26 6 (M+) , 175 and 91. IR (CHC1J 1677 , 1603 , 1491 cm1. 1H-NMR(CDC1^, 300 MHz) delta(ppm) :
1.70 (m, 1H) , 2.03 (m, IK), 2.5-2.9 (m, 4H) , 3.42 (dd, J=12, 3 Hz, 1H), 3.79 (s, OCHj), 6.98 (dd, J=8, 2 Hz, ArH), 7.07 (d, J=8 Hz, ArH), 7.2 (m, 5ArH) and 7.49 (d, J=2 Hz, ArH).
EXAMPLE 137
2-Benzyl-3,4-dihydro-7-hydroxy-l(2H)-naphthalenone
A mixture of 9.00 g (33.8 mmol) of the title product of the preceding Example in 32 ml acetic acid and 32 ml concentrated H3r was heated at reflux for 8 hours. The reaction was cooled and slowly added to 150 ml ice and water. The precipitated needle crystals were collected by filtration and dried in vacuo to give 8.46 g (99%) of the title compound. A portion was recrystallized from ether/hexane, 158-160° C. (dec.).
MS (m/e) 252 (M+), 174, 161, 133, 115, 106 and 91. IR (KBr) 1677 , 1610 cm1. 1H-NMR (CDCl-j-DMSO-dg, 300 MHz) delta(ppm) : 1.57 (m, 1H) , 1.90 (m, 1H), 2.4-2.7 (m, 4H), 3.28 (dd, J=14, 3 Hz, 1H), 6.83 (dd, J=8, 2 Hz, ArH), 6.91 (d, J=8 Hz, ArH), 7.08 (m, 5 ArH), 7.33 (d, J=2 Hz, ArH) and 8.86 (s, OH).
Analysis calculated for Ο^^Η^θΟ^:
C, 80.93; H, 6.39%.
Found: C, 81.25; H, 6.16%.
BAD ORIGINAL &
-35EXAMPLE 133
2-Benzyl-7-(2-quinolyl)methoxy3,4-dihvdro-l·(2H)-naphthalenone
A mixture of 3.87 g (15.4 mmol) of the title 5 product of the preceding Example, 4.7 g (22.9 mmol)
2-chloromethvlquinoline, 17.9 g (54.9 mmol) cesium carbonate hnd 220 mg (0.849 mmol) cesium iodide in 33 ml acetone was heated at reflux for 15 hours. The reaction was cooled, diluted with ether and filtered.
Evaporation of the filtrate gave an oil. This crude product was crystallized from ether-hexane to give 4.17 g (69%) cf the title compound, m.p. 117-118° C.
MS (m/e) 393 (M+), 143, 142, 115 and 91. IR (CKC13) 1678, 1604, 1568 cm1. 1H-NMR(CDC13, 300
MHz)delta(ppm): 1.72 (m, 1H), 2.06 (m, 1H), 2.5-3.0 (m, 4H), 3.46 (m, 1H), 5.40 (s, OCH3), 7.2 (m, 7H),
7.52 (dd, J=8, 8 Hz, lArH), 7.65 (m, 3ArH), 7.80 (d,
J=8 Hz, ArH), 8.07 (d, J=8 Hz, ArH) and 8.17 (d, J=8 Hz , ArH) .
Analysis calculated for
C, 82.42; K, 5.89; N, 3.56%.
Found: C, 82.32; H, 5.91; N, 3.51%,
EXAMPLE 139 cis- and trans-2-Benzyl-l,2,3,4-tetra25 · hydro-7-(2-quinolyl)methoxy-l-naphthol
To a 0° C. solution of 3.00 g (7.63 mmol) of the title product of the preceding Example in 100 mi 1:1 methanol: tetrahydrofuran was added portionwise 1.5 g (39.5 mmol) of sodium borohydrice and the reaction stirred for 15 hours. The reaction was concentrated on a rotating evaporator and the residue dissolved in a mixture of ethyl acetate and saturated NaCl. The δρ η n 0 0 8 3 bad ORIGINAL $
-86ο η γ» Π A organic layer was washed with saturated NaCl, dried over MgSO4 and evaporated to a solid, which was purified via medium pressure liquid chromatography on silica gel eluted with 66% ether-hexane to give in orier of elution
1.09 g (36%) of cis-title product and 1.68 g (56%) of trans-title product as oils. Crystallization was achieved from diisopropyl ether-dichloromethane to give 850 mg of the cis and 1.30 g of the trans isomer.
cis-isomer. m.p. 113-115° C.; MS (m/e) 395 (M+) ,
286 , 142 , 130 , 115 and 91. IR (CHCl-j) 3596, 3435,
1602, 1576 cm1. 1H-NMR(CDCl^, 300 MHz)delta(ppm): 1.5-1.9 (m, 3H), 1.96 (m, 1H) , 2.5-2.84 (m, 2H and OH), 2.90 (m, 1H) , 4.42 (bs, OCH) , 5.31 (s, 00Η?), 6.96 (dd, J=8, 2 Hz, ArH) , 6.92 (d, J=2 Hz, ArH) , 6.99 (J=8 Hz,
ArH), 7.22 (m, 5ArH), 7.50 (dd, J=8, 8 Hz, ArH), 7.60 (d, J=8 Hz, ArH), 7.69 (dd, J=8, 8 Hz, ArH), 7.78 (d,
J=8 Hz, ArH), 8.03 (d, J=8 Hz, ArH) and 8.13 (d, J=8 Hz, ArH).
Analysis calculated for C_,7H25NO2:
C, 82.00; K, 6.37; N, 3.54%
Found: C, 82.11; H, 6.36; N, 3.50% trans-isomer. m.p. 112-113° C.; MS (m/e) 395 (M+), 304, 303, 2S6, 143, 142, 115 and 91. IR (CHCip 3578, 3350, 1601, 1576 cm1. 1K-NMR(CDC13, 300
MHz)delta(ppm) : 1.20 (m, 1H) , 1.9 (m, 3H) , 2.43 (dd,
J=12, 8 Hz, IK), 2.62 (m, 1H and CH), 3.03 (dd, J=13, 5 Hz, 1H), 4.19 (dd, J=7, 7 Hz, OCH), 5.32 (s, OCH2),
6.92 (dd, J=8, 2 Hz, ArH), 6.94 (d, J=8 Hz, ArH), 7.15 (m, 5ArH), 7.48 (dd, J=8, 8 Hz, ArH), 7.62 (d, J=8 Hz,
ArH), 7.62 (dd, J=8, 8 Kz, ArH), 7.76 (d, J=8 Hz, ArH), 8.02 (d, J=8 Kz, ArH) and 8.12 (d, J=8 Hz, ArH).
Analysis calculated for ^27^25^^21
C, 82.00; H, 6.37; N, 3.54%
Found: C, 82.18; H, 6.39; N, 3.49%
BAD ORIGINAL &
EXAMPLE 140 trans-2-Benzy1-1,2,3,4-tetrahydro-7(2-quinolyl) methoxy-1-naphthoi Hydrochloride
To a 0° C. solution of 1.00 g (2.53 mmol) of trans-title product of the preceding Example in 100 ml ethanol was added 2.53 ml of IN hydrochloric acid (2.53 mmol) . The reaction solvent was evaporated on a rotating evaporator, and the residue diluted with 100 ml ethanol and evaporated, a process which was repeated 3X to give dry material. The residue was crystallized from dichloromethane-ether to give 855 mg (78%) of the title compound, m.p. 183-185° C.
1H-NMR(CDC13, 300 MHz)delta(ppm): 1.40 (m, IK),
1.8-2.05 (m, 2H), 2.47 (dd, J=13, 8 Hz, 1H), 2.73 (m,
2H) , 3.19 (dd, J=14, 4 Hz, 1H) , 4.50 (d, J=7 Hz, OCH) ,
5.85 (d, J=15 Hz, OCH2 1H), 5.99 (d, J=15 Hz, OCH2 1H),
6.86 (dd, J=8, 2 Hz, ArH), 6.97 (d, J=8 Hz, ArH), 7.26 (m, 5ArH), 7.54 (d, J=2 Hz, ArH), 7.86 (dd, J=8, 8 Hz, ArH), 8.08 (m, 3ArH), 8.75 (d, J=8 Hz, ArH) and 8.98 (d, J=8 Hz, ArH).
EXAMPLE 141
1R,2R- and IS ,2S-(trans)-2-Benzyl1,2,3,4-tetrahydro-7-(2-quinolyl)methoxy-1-naphthyl R-O-Acetylmandelate
To a 0° C. solution of 2.00 g (10.3 mmol) of (R)-O-acetylmandelic acid and 1.26 g (10.3 mmol) of 4-N,N-dimethylaminopyridine in 16 ml CH2C12 was added 3.40 g (8.60 mmol) of trans-title product of Example 139, followed by 1.95 g (9.46 mmol) of dicyclohexylcarbodi30 imide. The reaction was then stirred at 25° C. for 24 hours. The reaction was filtered and the filtrate evaporated to a crude oil. This oil was purified via column chromatography on 500 g of silica gel eluted with 50% ether-hexane to give in order of elution 1.93 g £ 8 0 0 0 0 dV bad ORIGINAL
Ο 20
-S3(39%) o: the 1£ the lS,2S-tit!e from ether/hexane.
IR, 2R-diastereomer
R-titie product and 2.03 g (41%) of rcduct. The former was crystallized
m.p. 150-151° C.; MS (m/e) 571 (M+) , 378 , 296, 286, 261 , 241 , 142 , 125 , 111, 97 l;
and 85. IR (CHCl^) 1741, 1602, 1600 i - J
300 MHz)delta(ppm): 1.28 (m, 1H) , 1.94 (m, 1H), 2.22 (s, CH3CO) , 2.24 (m, 1H) , 2.36 (m, lH) , 2.66 (m, 2H) , 2.96 (dd, J = 13, 3 Hz, 1H) , 4.94 (d, J=15 Hz, OCH2 1H) , 5.04 (d, J=15 Hz, CCH2 1H) , 5.90 (d, J=7 Hz, OCH) , 5.94 (s, OCH), 6.36 (d, J = 2 Hz, ArH), 6.81 (dd, J=8, 2 Hz,
ArH), 6.96 (d, C = 3 Hz, ArH), 7.1-7.3 (m, 9ArH), 7.56 (m, 3Ar.H) , 7.75 (dd, J = 8 Hz, ArH), 7.85 (d, J=8 Hz, lArH), 8.11 (d, J = 8 Hz, lArH) and 8.20 (d, J=8 Hz,
ArH) .
Analysis calculated for C^H^NO^:
C, 77.74 ; H, 5.83; N, 2.45%.
Found: C, 78.30; H, 5.87; N, 2.41%.
IS ,2S-diastereomer 1 cm
H-NMR(CDC1
3'
H-NMR(CDC13, 300
MHz) delta (ppm) : 1.20 (m, 1H) , 1.77 (m, 1H) , 1.97 (m,
1H) , 2.15 (m, IK), 2.26 (s, CK-jCO) , 2.45 (dd, J=15, 2 Hz, 1H) , 2.65 (m, 2H) , 5.36 (AS pattern, OCH2), 5.91 (d, J=7 Hz, OCH), 5.98 (s, OCH), 6.85-7.04 (m, 5ArH),
7.2 (m, 4ArH), 7,38 (m, 3ArH), 7.55 (m, 2ArH), 7.72 (m, 2ArH), 7.84 (d, J = 3 Hz, lArH), 3.11 (d, J=8 Hz, ArH) and 8.21 (d, J=3 Hz, ArH).
BAD ORIGINAL
-89EXAMPLE 142
1R, 2R-2-Benzy1-1,2,3,4-tetrahydro7 - (2-quinoly I) rnethyl-l-naphthol
A mixture of 1.70 g (2.98 mmol) of lR,2R-title product of the preceding Example and 4.75 g (34.4 mmol) of anhydrous K^CO^ in 35 ml tetrahydrofuran, 35 ml methanol and 9 ml water was stirred at 25° C. for 20 hours. The reaction was concentrated on a rotating evaporator and the residue dissolved in a mixture of
300 ml water and 100 ml ether. The combined organic layer and two further 100 ml ether extracts were dried over MgSO^ and evaporated. The resultant solid was recrystallized from C^Cl^/hexane to give 1.10 g (93%) of present title compound, m.p. 130-132° C.
IR (CHCip 3580, 3387, 1602, 1580 cm'1. MS (m/e)
395 (M+), 376, 366, 348, 303, 286, 253, 143, 142, 115 and 91. 1H-NMR(CDC13, 300 MHz)delta(ppm): 1.25 (m,
1H), 1.80 (d, J=7 Hz, OH), 1.84-2.6 (m, 2H), 2.48 (dd, J=16, 10 Hz, 1H), 2.67 (m, 2H), 3.07 (dd, J=15, 6 Hz,
1H), 4.43 (dd, J=7, 7 Hz, OCH), 5.37 (s, OCH2), 6.85 (dd, J=8, 2 Hz, ArH), 6.98 (d, J=8 Hz, ArH), 7.1-7.4 (m, 6ArH), 7.52 (dd, J=8, 8 Hz, ArH), 7.66 (d, J=8 Hz, ArH), 7.71 (cd, J=8, 3 Hz, ArH), 7.81 (d, J=8 Hz, ArH), 8.06 (d, J=8 Hz, ArH) and 8.16 (d, J=8 Hz, ArH).
Analysis calculated for ^27^25^^2:
C, 82.00; H, 6.37; N, 3.54%.
Found: C, 81.90; H, 6.42; N, 3.51%.
[alpha]2Q = -55.3° (methanol c=0.01).
bad original
£ 8 0 0 0 0 dV
-90vi ,χ ··>· ' v, tt
->
m/ ώ
>
EXAMPLE 143
IS , 2S-2-Benzy1-1,2,3,4-tetrahydro7-(2-cuinolvl)methoxv-l-naohthol * * -- . X. Using the method of the preceding Example, 700 mg 5 (1.23 mmol) of the lS,2S-title product of Example 141 gave 393 mg (81%) of present title compound, recrystallized from dichloromethane/diisopropyl ether, m.p. 131-132? C.
IR (CHC13) 3581, 3375, 1602, 1493 cm1. MS (m/e)
395 (M+), 376, 303, 286, 253, 143, 142, 115 and 91.
[alpha]^ = +55.26° (methanol c=0.01026).
EXAMPLE 144 trans-2-Benzy1-1,2,3,4-tetrahydro7-(2-quinolyl)methoxy-l-naphthy1
4-P iperidinobutyrate Dihydrochloride
To a 0° C. solution of 935 mg (4.52 mmol) of
4-piperidinobutyric acid hydrochloride, 733 mg (6.01 mmol) 4-(N,N-dimethylamino)pyridine and 1.49 g (3.77 mmol) of the trans-title product of Example 139 in 7.5 ml was added 852 mg (4.14 mmol) dicyclohexylcarbodiimide. The resultant reactio-n mixture was stirred 15 hours at 25° C., then filtered and the filtrate evaporated to an oil. The latter was purified via column chromatography on 120 g silica gel eluted with 10% methanol-CH^Cl^ to yield a second oil, which was dissolved in 100 ml ethanol. IN Hydrochloric acid (7.54 ml) was added, and the reaction concentrated to dryness on a rotating evaporator. The residue was twice dissolved in 100 ml ethanol and evaporated to give a solid. This solid was'recrystallized from
CH2C12 and diisopropyl ether to give 2.00 g (85%) of the title compound, m.p. 132-135° C.
BAD ORIGINAL
-91IR (X3r) 1745, 1643, 1606 cm1. MS (m/e) 549 (M+-2HC1), 405, 236, 170, 142, 98 and 91.
XH-NMR(DMSO-dg, 300 MHz) delta(ppm) : 1.3-2.1 (m) ,
2.1-3.0 (m), 3.36 (m, 2H), 5.47 (s, OCH2), 5.73 (d, J=7 Hz, OCH), 6.80 (d, J=2 Hz, ArH), 7.00 (dd, J=8, 2 Hz, ArH) , 7.10 (d, J=8 Hz, ArH), 7.13-7.32 (m, 5ArH), 7.73 (dd, J=8 , 8 Hz, ArH), 7.81 (d, J=8 Hz, ArH), 7.92 (dd, J=8, 8 Hz, ArH), 8.12 (d, J=8 Hz, ArH), 8.18 (d, J=8 Hz, ArH) and 8.66 (a, J=8 Hz, ArH).
Analysis calculated for C3gK42C^2N2O3*1 *5H2O:
C, 66.66; H, 6.99; N, 4.32%.
Found: C, 66.20; H, 6.82; N, 4.23%.
EXAMPLE 145
3,4-Dihydro-7-(2-quinoly1)methoxy-1(2H)-naphthalenone
By the method of Example 138, 5.00 g (30.9 mmol) of 7-hydroxy-3,4-dihydro-l(2H)naphthalenone and 9.91 g (46.3 mmol) of 2-chlorcmethvlquinoline hydrochloride gave 3.5 g (37%) of the title compound.
MS (m/e) 303 (M+), 286, 274, 142, and 115. 1H-NMR(CDC13, 300 MHz)delta(ppm): 2.08 (m, 2H), 2.60 (t, J = 7 Hz, CH2) , 2.87 (t, J=6 Hz, CH^, 5.39 (s,
OCH2), 7.16 (d, J=2 Hz, ArH), 7.52 (dd, J=8, 8 Hz,
ArH), 7.6-7.75 (m, 4ArH), 7.79 (d, J=8 Hz, ArH), 8.07 (d, J=8 Hz, ArH) and 3.16 (d, J=8 Hz, ArH).
ΔΡ0 0 0 0 8 3
BAD ORIGINAL
-92EXAMPLE 146
2- (3-Pyridylmethylene)-7- (2-quinolyl) methoxy-3,4-dihydro-1(2H) -naohthalenone
A solution of 3.50 g (11.5 mmol) of the title 5 product of the preceding Example, 2.47 g (23.1 mmol) of
3-pyridine carbaldehyde and 1.9 ml (23 mmol) of pyrrolidine in 12 ml methanol was heated at reflux for 22 hours. The reaction was cooled and added to 300 ml saturated NaCl and 150 ml ethyl acetate. The organic layer was combined with two further 150 ml ethyl acetate extracts, dried over MgSO^, evaporated to dryness and the residue triturated with ether to give 2.95 g (66%) of the title compound, m.p. 147-148° C.
MS (m/e) 392 (M+) , 363 , 300 , 14 2 , and 115. IR (CHC13) 1667, 1600, 1566 cm1. 1H-NMR(CDC13, 300
MHz)delta(ppm) : 2.90 (t, J=7 Hz, 2H) , 3.08 (t, J = 7 Hz, .i 2H) , 5.43 (s, OCH2) , 7.1-7.4 (m, 3ArH) , 7.54 (dd, J=8,
Hz, ArH), 7.4-7.9 (m, 6ArH), 8.08 (d, J=8 Hz, ArH), ' 8.19 (d, J=8 Hz, ArH), 8.54 (d, J=2 Hz, ArH) and 8.66 & . 20 (s, vinyl H) .
> EXAMPLE 147
2-(3-Pyridylmethyl)-7-(2-quinolvl)methoxv-3,4-dihydro-l(2H)-naphthalenone and cis-2-(3-Pyridylraethyl)-7-(2-quinolyl)2 5 methoxy-1,2,3,4-tetrahvdro-l-naphthol·
A mixture of 3.30 g (8.42 mmol) of the title product of the preceding Example and 500 mg of 10% Pd/C in 177 ml of tetrahydrofuran was stirred under 1 atmosphere of hydrogen. When hydrogen uptake ceased, the reaction was faltered and the filtrate evaporated to a solid. Column chromatography of this solid on 500 g of silica gel using 25% acetonitrile-dichloromethane as eluant gave in order of elution 1.35 g (41%) of ketonic title product as a solid, m.p. 115-117° C., and 350 rag (11%) of the cis-naphthol title product, recfystallized from dichloromethane, m.p. 157-160° C.
BAD ORIGINAL &
-93EXAMPLE 143 cis- and trans-1,2,3,4-Tetrahydro-2-(3-pyridvlmethv1) - 7 - ( 2 -quinolyl) methoxy-l-naphthol
To a 0° C. solution of 1.35 g (3.43 mmol) of the ketonic title product of the preceding Example in 45 ml of 1:1 methanol: tetrahydrofuran was added 320 mg (8.57 mmol) of NaBH^. The reaction was stirred for 2 hours and then added to 200 ml saturated NaCl and 150 ml
CH2Cln. The organic layer was combined with two additional 150 ml Ch’2Cl2 extracts, dried over MgSO^, and evaporated to a solid, which was purified via column chromatography on 500 g of silica gel eluting with 10% isopropyl alcohol-CHnCl0 to give in order of
4. 4b elution the title-cis-isomer, identical with the cisisomer of the preceding Example, and the title-transisomer as an oil.
EXAMPLE 149
2-Benzylidene-6-methoxy-l-indanone
To a 0° C. mixture of 9.66 g (59.6 mmol) of 6-methoxy-l-indanone and 6.32 g (59.6 mmol) of benzaldehyde in 10 ml ethanol was added 9.66 ml of a 4% KOH in ethanol solution. The reaction was stirred 1 hour and then added to 300 ml water and the pH of the quench adjusted to 2 with IN hydrochloric acid. The resultant mixture was extracted with 3 x 300 ml ether, and the extracts combined, dried over MgSO^, evaporated and the residue triturated with ether to yield 10.8 g (72%) of present title compound as a solid.
1H-NMR(CDC13 , 300 MHz)delta (ppm) : 3.80 (s, OCH3), 3.92 (s, CH2), 7.13 (dd, J=8, 2 Hz, ArH), 7.35 (m, 4ArH and vinyl H) and 7.6 (m, 3ArK).
ad η nn Π ft
BAD ORIGINAL ft
-94EXAMPLS 150
2-Benzyl-6-methoxy-1-indanone By the method of Example 147, 9.94 g (39.8 mmol) of the title product of the preceding Example and 5 1.00 g of 10% Pd/C in 500 ml ethyl acetate was converted to 8.04 g (80%) of present title compound as a solid from hexane.
MS (m/e) 252 (M+), 161 and 91. 1H-NKR(CDC13, 300 MHz)delta(ppm) : 2.62 (dd, J=13, 10 Hz, 1H) / 2.74 (d,
J=13 Hz, IK), 2.92-3.12 (m, 2H), 3.34 (dd, J=16, 3 Hz,
1H) , 3.80 (s, OCh'3), 7.08-7.5 (m, 8ArH).
EXAMPLE 151
2-Benzyl-6-hydroxy-1-indanone By the method of Example 137, 8.00 g (31.7 mmol) of the title product of the preceding Example gave
7.44 g (97%) of present title compound, recrystallized from water-acetic acid, m.p. 140-143° C.
MS (m/e) 238 (M+) , 161, 147 and 91. IR (CHCl-j) 3665, 3583, 1701, 1618, 1602 cm-1. 1H-MMR(CDC13, 300
MHz) delta(ppm) : 2.62 (dd, J=15, 12 Hz, 1H) , 2.74 (d,
J=15 Hz, 1H), 2.93-3.13 (m, 2H), 3.34 (dd, J = 12, 3 Hz, 1H) , 7.10 (dd, J=8, 2Hz, ArH) and 7.23 (m, 7ArH).
EXAMPLE 152
2-Benzyl-6-(2-quinolyl)methoxy-1-indanone
By the method of Example 138, 3.50 g (14.7 mmol) of the title product of the preceding Example and 4.80 g (22.4 mmol) of 2-chloromethylquinoline hydrochloride gave 890 mg (16%) of present title compound, recrystallized from ether, m.p. 104-106’ C.
MS (m/e) 379 (M+), 344, 143, 115 and 91. IR (CKC13) 1705 , 1617 , 1603 , 1567 cm1. 1H-NMR(CDC13, 300 MHz) delta(ppm) : 2.60 (dd, J=13, 10 Hz, 1H) , 2.74 (dd,
J=16, 3Kz, 1H), 2.9-3.1 (m, 2H), 3.33 (dd, J=ll, 3 Hz, 1H), 5.36 (s, OCH3), 7.1-7.4 (m, 8ArH), 7.51 (dd, J=8, bad ORIGINAL &
-958Kz, ArH), 7.59 (d, J=3 Hz, ArH), 7.70 (dd, J=8, 8 Hz, ArH), 7.79 (d, J=8 Hz, ArH), 8.05 (d, J=8 Hz, ArH) and 8.15 (d, J=3 Hz, ArH).
EXAMPLE 153 cis- and trans-2-Benzyl-6-(2quinolyl)methoxy-l-indanol
By the method of Example 139, 0.89 g (2.35 mmol) of the title product of the preceding Example gave in order of elution 220 mg (25%) of the cis-title product,
m.p. 138-139° C., and 459 mg (52%) of the trans-title product, m.p. 137-138’ C.
cis-isomer. MS (m/e) 381 (M+) , 272 , 142, 115 and
91. IR (CHC13) 3593, 3432, 1613, 1603, 1584, 1567 cm1 1H-NMR(CDC13 , 300 MHz) delta (ppm) : 2.6-2.8 (m, 4H) ,
3.07 (dd, J=13, 6 Hz, 1H), 4.92 (bs, with D20; d, J=6 Hz, OCH) , 5.37 (s, OCH^ , 6.92 (dd, J=8, 2 Hz, ArH), 7.04 (d, J=2 Hz, ArH), 7.11 (d, J=8 Hz, ArH), 7.15-7.4 (m, 5ArH), 7.54 (dd, J=8, 2 Hz, ArH), 7.65 (d, J=8 Hz, ArH), 7.72 (dd, J=8, 8 Hz, ArH), 7.81 (d, J=8 Hz, ArH), 8.07 (d, J=8 Hz, ArH) and 8.17 (d, J=8 Hz, ArH). Analysis calculated for ^26^23^°2:
C, 81.86; H, 6.08; N, 3.67%
Found: C, 81.86; H, 6.00; N, 4.06%.
trans-iscmer. MS (m/e) 381 (M+), 272, 142, 130, 115 and 91. IR (CHCl-j) 3585 , 3436 , 1614 , 1602, 1568 cm 1H-NMR(CDC13 , 300 MHz)delta (ppm) : 2.5 (m, 2H), 2.76 (dd, J=15, 9 Hz, 1H), 2.9 (m, 1H), 3.06 (dd, J=12, 6 Hz, 1H) , 4.88 (bs, with D2<0; d, J=6 Hz, OCH), 5.36 (s, 0CHo), 6.87 (dd, J=8, 2 Hz, ArH), 7.02 (m, 2ArH), 7.17.4 (m, 5ArH), 7.52 (dd, J-8, 2 Hz, ArH), 7.64 (d, J=8 Hz, ArH), 7.71 (dd, J=8, 8 Hz, ArH), 7.80 (d, J=8 Hz,
AP 0 0 0 0 8 3
ArH), 8.06 (d, J=8 Hz, ArH) and 8.16 (d, J=8 Hz, ArH). Analysis calculated for C26H23^°2:
Found :
C, 81.86; H, 6.08; N, 3.67% C, 81.51; H, 6.05; N, 3.57%
BAD ORIGINAL ft
-96EX AMPLE 154
2-5enzyl-6- ( 2-pyridyl) methcxy-l-indanone By the method of Example 138, 3.50 g (14.7 mmol) of the title product of Example 151 and 3.61 g (22.0 5 mmol) of 2-chloromethylpyridine hydrochloride were converted to 3.40 g (69%) of present title compound, recrystallized from CH^^-hexane, m.p. 94-97° C.
MS (m/e) 329 (M+), 314, 300, 238, 93, 92 and 91.
IR (CHC1-J 17C4, 1617, 1595, 1574 cm1. 1H-NMR(CDC1.,
J j
300 MHz)delta(ppm): 2.61 (dd, J=15, 11 Hz, 1H), 2.73 (dd, J=15, 4 Hz, IK), 2.9-3.1 (m, 2H), 3.34 (dd, J=12,
Hz, 1H) , 5.19 (s, OCh’2), 7.1-7.4 (m, 9ArH) , 7.44 (d, J=8 Hz, ArH), 7.67 (dd, J=8, 8 Hz, ArH) and 8.56 (J=5 Hz , ArH) .
* 15 EXAMPLE 155 v cis- and trans-2-Benzyl-6(2-pyridyl)-methoxy-l-indanol
By the method of Example 139, the title product of the preceding Example (3.40 g, 10.3 mmol) was converted p 20 to present title products, giving in order of elution £> 810 mg (24%) of cis-title product, m.p. 124.5-125.5° C.
and 1.32 g '(39%) of trans-title product, m.p.
112-113° C., both recrystallized from CH2C12/isopropyl ether .
cis-isomer. MS (m/e) 331 (M+), 313, 240, 222, 115 and 91. IR (CHC13) 3591, 1613, 1596, 1574 cm1.
1H-NMR(CDC13 , 300 MHz) delta(ppm) : 2.75 (m, 4H) , 2.09
(dd, J=12, 6 Hz, 1H), 4.95 (d, J = 6 Hz, OCH) , 5.20 (s,
OCH2), 6.90 (dd, J=8, 2 Hz , ArH), 7.02 (d, J=2 Hz,
ArH), 7.12 (d, J=8 Hz, ArH) , 7.2-7.4 (m, 6 ArH), 7.52
(d, J=8 Hz, ArH), 7.72 (dd, J=8, 8 Hz, ArH) and 8.57
(d, J=5 Hz, ArH) .
Analysis calculated for C^H^NC^:
C, 79.73; H, 6.39; N, 4.23%.
Found: C, 79.38; H, 6.32; N, 4.11%
BAD ORIGINAL
-97trans-isomer. MS (m/e) 331 (M+,, 240, 239, 222,
148 and 91. IR (CHCi-j) 3586 , 34 1 1 , 1613 , 1596 ,-1575 cm1 1H-NMR(CDC13, 300 MHz)delta(ppm): 2.55 (m, 2H), 2.77 (dd, J=14, 8 Hz, 1H), 2.92 (m, 1H), 3.09 (dd, J=12, 6 Hz, 1H), 4.89 (d, J=6 Hz, OCH), 5.19 (s, OCH2), 6.85 (dd, J=8, 2 Hz, ArH), 7.00 (d, J=2 Hz, ArH), 7.05 (d,
J=8 Hz, ArH), 7.15-7.4 (m, 6 ArH), 7.51 (d, J=8 Hz,
ArH), 7.70 (dd, J=8, 8 Hz, ArH) and 8.56 (d, J=5 Hz,
ArH) .
Analysis calculated for C22H211JO2:
C, 79.73; K, 6.39; N, 4.23%.
Found: C, 79.67; H, 6.37; N, 4.16%.
EXAMPLE 156
3,4-Dihvdro-7-methoxy-2-phenoxy-l(2H)-naphthalenone
A mixture of 2.95 g (31.4 mmol) of phenol, 25.5 g (78.2 mmol) of cesium carbonate and 320 mg (1.23 mmol) of cesium iodide in 64 ml of acetone was heated at reflux for 40 minutes and then cooled to 0° C. To this 0° C. mixture was added 8.00 g (31.4 mmol) of 2-bromo3,4-dihydro-7-methoxy-l(2K)-naphthalenone. The resultant reaction mixture was stirred 3 hours at 0° C. and 15 hours at 25° C., then filtrated. The filtrate was evaporated to an oil which was purified via column chromatography on 400 g of silica gel, eluting with 25% ether-hexane, to give 5.14 g (61%) of present title product as an oil, crystallized from ether-hexane, m.p. 94.5-96.5° C.
MS (m/e) 268 (M+), 174, 173, 160, 147, 131, 120
115 and 103. IR (CHCl^) 1696, 1610, 1598 cm1.
I J
H-NMR(CDC13)delta(ppm): 2.3-2.7 (m, 2H), 3.0-3.4 (m,
2H), 3.82 (s, OCH3), 4.88 (dd, J=8, 6 Hz, OCH) and
6.9-7.6 (m, 8ArH) .
Analysis calculated for 0^7Η^,03
Found:
C, 76.10; H, 6.01%. C, 75.75; H, 5.95%.
BAD ORIGINAL
-98Ζ» :'ϊ /»
EXAMPLE 157 cis- and trans-1,2,3,4-Tetrahvdro7-methoxy-2-phenoxy-l-naphthol
Sy the method of Example 139, 2.86 g (10.8 mmol) of title product of the preceding Example gave 2.88 g (100%) of a mixture of present .title products as a solid.
MS (m/e) 270 (M+), 176, 159, 147 and 121. IR (CHCl-j) 3560 , 161 1 , 1597 , 1583 cm1. 1H-NMR(CDCi^, 300
MHz)delta(ppm) : 1.9 (m, IH) , 2.3 (m, IH) , 2.65 (m,
1H) , 2.85 (m, IH), 3.79 (s, 0CH3), 4.45 , 4.69 , 4.85 (m, 2H), 6.79, 7.0, 7.25 (m, 8ArH).
EXAMPLE 158 cis- and trans-2-Phenoxy-l,2,3,415 tetrahydro-1,7-naphthalenediol
To a solution of 2.80 g (10.4 mmol) of the title products of the preceding Example in 5 ml of hexamethy1phosphoramide (distilled in vacuo from sodium was added 15.66 ml (about 42 mmol) of a solution of lithium n-propylmercaptide (made from 74.6 mmol of propanethiol in 75 ml of tetrahydrofuran and 44 ml of 1.6N n-butyllithium in hexane, followed by dilution with 70 mi hexamethyiphosphoramide). The reaction solution was heated at 105° C. for 4.5 hours and then stirred for 15 hours at 25° C. The reaction was added to 200 ml water containing 2.6 ml concentrated hydrochloric acid. The quenched reaction was extracted with two 100 ml portions of ether. The combined ether extracts were washed with water and saturated NaCl, dried over MgSO^, and evapo30 rated to a solid. Recrystallization from benzene gave 1.94 g (73%) of present title products as a mixture of cis and trans-isomers, m.p. 140-157° C.
-99MS (m/e) 256 (M ), 162, 145 and 133. IR (RBr)
1615, 1597, 1588 cm ’H-NMR(CDC13, 300
MHz)delta(ppm):
1.85 (m, 1H), 2.18 (m, 1H), 2.5-2.9 (m, 2K), 4.39, 4.55, 4.7 (m, 2H), 6.64, 6.85, 7.15 (m, 8ArH).
Analysis calculated for
C, 74.98; H, 6.29%.
Found: C, 74.79; H, 6.26%.
EXAMPLE 159 cis- and trans-2-Phenoxy-7-(2-quinolyl)methoxy-1,2,3,4-tetrahydro-l-naphthol
By the method of Example 138, 1.82 g (7.16 mmol) of the title product of the preceding Example and 2.30 g (10.8 mmol) of 2-chloromethyIquinoline hydrochloride gave 2.2 g of a cis-trans mixture of the title compounds after silica gel column chromatography. Separation of isomers was achieved via HPLC on a 21.4 mm x 25 cm Dynamax Macro HPLC Si column eluted with 15% ether-dichloromethane (16 ml/min) to give the less polar cis-isomer (186 mg, 6%) and the more polar trans-isomer (28 mg, 1%) as pure fractions.
cis-isomer. m.p. 118° C.; MS (m/e) 397 (M+) , 304 , 286, 274, 143, 142, 116 and 115. IR (CHCl-j) 3562,
1609, 1599 , 1583 cm-1. 1H-NMR(CCC13, 300 MHz) delta(ppm) 2.0 (m, 1H), 2.33 (m, 1H), 2.7 (m, 1H), 2.93 (m, 1H), 4.71 (ddd, J=3.02, 3.02, 8.44 Hz, ArOCH), 4.87 (s,
OCH), 5.39 (s, OCH2), 6.9-7.1 (m, 5ArH), 7.15-7.35 (m, 3ArH), 7.54 (dd, J=8, 8 Hz, ArH), 7.7 (m, 2ArH), 7.83 (d, J=8 Hz, ArH), 8.08 (d, J=3 Hz, ArH) and 8.19 (d,
J=8 Hz, ArH).
Analysis calculated for Cn,K^^NO,:
J 26 z3 3
C, 78.57; H, 5.83; N, 3.52%.
Found: C, 78.30; H, 5.82; N, 3.33%.
ADO Ω 0 0 8 3 bad original
-100trans-isomer . (CDCl^, 300 MHz) delta(ppm) :
l. 85 (m, 1H) , 2.22 (m, 1H) , 2.32 (m, 2 Ii), 4.44 (ddd, J=2.77, 6.85, 9.82 Hz, ArOCH), 4.84 (d, J=7 Hz, OCH), 5.35 (s, OCH^), 6.3-7.1 (m, 5ArH), 7.24 (m, 3ArH), 7.50 (dd, J=8, 8 Hz, ArH), 7.65 (m, 2ArH), 7.78 (d, J=S Hz, ArH), 8.04 (d, J=8 Hz, ArH) and 8.14 (d, J=8 Hz, ArH).
EXAMPLE 160
3(2H)-Benzylidene-6-methoxy-l-(g-toluenesulfonyl) -4 (1H) -quinolinor.e
By the method of Example 146, 25.0 g (75.5 mmol) of 6-methoxy-l-(d-toluenesulfonyl)-2,3-dihydro-4(1H)quinolinone (J. Am. Chem. Soc., Vol. 71, p. 1901, 1949) and 12.0 g (113 mmol) of benzaldehyde were converted to 20.0 g (63%) of present title product recrystallized from ether-hexane, m.p. 131-132° C.
MS (m/e) 419 (M+) , 264 and 221. IR (CHCi-j) 1672 ,
1599 , 1569 cm1. 1H-NMR(CDC13) delta(ppm) : 2.32 (s,
ArCH3), 3.83 (s, OCH3) , 5.05 (d, J=2H, CH2), 7.0-7.6 (m, lOArH, vinyl H) and 7.75 (d, J=8 Hz, ArH).
Analysis calculated for C^H^NO^S:
C , 6 8.72 ; H, 5.05; N , 3.3 4 % .
Found; C, 68.85; H, 4.98; N, 3.17%.
EXAMPLE 161
6-Methoxy-l-(p-toluenesu1fony1) 2 5 3-benzyl-2,3-dihvdro-4(1H)-quinolinone
By the method of Example 147, 19.9 g (47.4 mmol) of the title product of the preceding Example was converted to 13.8 g (69%) of present title compound,
m. p. 101-103° C.
MS (m/e) 421 (M+) , 266 and 91. IR (CKCl-j) 1683 ,
1604 cm”1. 1H-NMR(CDC13, 300 MHz)delta(ppm) ; 2.2-2.5 (m, 2H) , 2.38 (s, ArCH3) , 3.28 (dd, J = 15, 2 Hz, 1H) , 3.56 (dd, J=13, 12 Hz, 1H), 3.62 (s, OCH3), 4.24 (dd,
BAD ORIGINAL ft
-101J=15, 5 Hz, 1H), 7.0 (m, 2ArH), 7.11 (m, 2ArH), 7.25 (m, 6ArH), 7.39 (d, J=2 Hz, ArH) and 7.77 (d, J=8 Hz, ArH) .
Analysis calculated for C_ ,H_.oN0 ,S :
* 24 23 4
c, 68.39; H, 5.50; N, 3.32%.
Found: C, 68.52; H, 5.51; N, 3.28%.
EXAMPLE 162
6-Methoxy-3-benzy1-2,3-dihydro-4(1H)-quinolinone
A mixture of 9.71 g (23.1 mmol) of the title product of the preceding Example in 78 ml acetic acid and 48 ml concentrated hydrochloric acid was refluxed for 8 hours. The reaction was added to 1 liter ice and water and the precipitate collected by filtration to yield 6.2 g (100%) of present title compound, m.p. 108-111° C.
MS (m/e) 267 <M+), 190, 176 and 91. IR (KBr)
1638 cm-1. 1H-NMR(CDC13)delta(ppm): 2.4-3.5 (m, 5H),
3.73 (s, OCH3), 6.64 (d, J=8 Hz, ArH), 6.96 (d, J=2 Hz, ArH) and 7.3 (m, 6ArH).
EXAMPLE 163
6-Hydroxy-3-benzy1-2,3-dihvdro-4 (1K)-quinolinone
By the method of Example 137, 6.16 g (23.0 mmol) of the title product of the preceding Example was converted to 2.52 g (43%) of the title compound, m.p. 143-149° C.
MS (m/e) 253 (M+), 176, 162 and 91. IR (K3r)
1640 cm”1. 1H-NMR(DMSO-d6 300 MHz)delta(ppm): 2.5-2.7 (m, 2H), 2.9-3.3 (m, 3H), 6.25 (s, NH) , 6.64 (d, J=8 Hz, ArH), 6.84 (dd, J=3, 2 Hz, ArH), 7.01 (d, J=2 Hz, ArH), 7.25 (m, 5ArH) and 8.31 (s, OH).
Analysis calculated for
C, 75.87; K, 5.97; N, 5.53%.
Found: C, 75.61; H, 5.94; N, 5.37%.
APO00083
BAD ORIGINAL
-102λ #
□ .S>
EXAMPLE 164 , 6-Hydroxy-1-(ο-toluenesui for.y1)-3benzvl-2,3-dihvdro-4(1H)-cuino1inene — 1 — ' >
To a solution of 2.19 g (8.66 mmol) of the title product of the preceding Example in 13 ml pyridine was added (gradually) 1.65 g (8.66 mmol) of £-toluenesulfonyl chloride. The reaction was stirred 1 hour and then added to 200 ml IN hydrochloric acid. The quenched mixture was extracted with ethyl acetate, and the organic extract washed with IN KC1 and saturated NaCl, dried over MgSO^, evaporated and the residue triturated with ether-hexane to give 2.88 g (82%) of present title compound, recrystallized from ether, m.p. 200-205° C.
MS (m/e) 407 (M+), 252 and 91. IR (KBr) 1687,
1604 cm1. 1H-NMR(CDC1-, + DMSO-d,, 300 MHz) delta (ppm) :
J o
2.1-2.3 (m, 2H), 2.32 (s, ArCH3), 3.20 (m, 1H), 3.47 (t, J=12 Hz, 1H), 4.15 (dd, J=14, 4 Hz, 1H), 6.8-7.3 (m, HArH), 7.61 (d, J=8 Hz, ArH) and 8.96 (s, OH). Analysis calculated for C23H21NO^S·}H^O:
C, 66.33; H, 5.32; N, 3.36%.
Found: C, 66.57; H, 5.29; N, 3.30%.
EXAMPLE 16 5 l-p-Toluenesulfonyl)-3-benzyl-6-(2-quinolyl)methoxy-2,3-dlhydro-4 (IF.) -cuinolinone
A mixture of 2.83 g (7.08 mmol) of the title product of the preceding Example, 1.38 g (7.79 mmol)
2-chloromethyIquinoline, 2.93 g (21.2 mmol) of anhydrous K2CO3 and 1.17 g (7.79 mmol) Nal in 7 ml acetone was heated at reflux for 20 hours. The reaction was cooled, filtered and the filtrate evaporated. Crystallization from ether-hexane gave
2.19 g (56%) of present title compound, m.p.
165-175° C.
bad original &
-103MS (m/e) 548 (M+), 393, 154, 142, 115 and 91. IR (K3r) 1687, 1604 cm-1. 1H-NMR(CDClg, 300 MHz)delta(ppm) 2.2-2.5 (m, 2H), 2.39 (s, ArCHg), 3.29 (dd, J=15, 2 Hz, 1H), 3.57 (t, J=15 Hz, 1H), 4.25 (dd, J=15, 5 Hz, 1H) ,
5.40 (s, OCH2), 7.03, 7.12 (m, 4ArH), 7.3 (m, 6ArH),
7.57 (m, 2ArH), 7.65 (d, J=8 Hz, ArH), 7.74 (dd, J=8, 8 Hz, ArH), 7.82 (m, 2ArH), 8.08 (d, J=8 Hz, ArH) and 8.21 (d, J=8 Hz, ArH).
EXAMPLE 166
IO 3-Benzy1-6-(2-cuinolyl)methoxy) 2,3-dihydro-4(1H)-auinolinone
A mixture of 2.05 g (3.74 mmol) of title product of the preceding Example, 13 ml of acetic acid, 8 ml concentrated hydrochloric acid and 2 ml water was heated at reflux for 9 hours. The reaction was added to 250 ml ice and water, adjusted to pH 6 with 6N NaOH (about 45 ml) and then adjusted to pH 8 by addition of solid NaHCOg. This mixture was extracted with CH2CI2 and the extract dried over MgSO^, evaporated and the residue triturated with ether to yield 350 mg (58%) of present title compound, m.p. 174-176° C.
MS (m/e) 394 (M+), 252, 143, 115 and 91. 1H-NMR(DMSO-d6, 300 MHz) delta (ppm) : 2.62 (m, 1H) ,
2.9-3.2 (m, 3H) , 3.29 (m, 1H) , 5.27 (s, OCH2), 6.51 (s,
NH) , 6.74 (d, J=8 Hz, ArH), 7.1-7.3 (m, 9 ArH)-, 7.6 (m, 2ArH), 7.76 (dd, J=3, 8 Hz, ArH), 7.93 (m, 2ArH) and 8.37 (d, J=8 Hz, ArH).
EXAMPLE 167 cis- and trans-3-Benzyl-6-(2-quinolyl)30 methoxy-1,2,3,4-tetrahydro-4-quinolinol
By the method of Example 139, 810 mg (2.06 mmol) of the title product of the preceding Example was converted to present title compound. Purification via column chromatography on 300 g of silica gel eluted
ΔΡ000083
BAD ORIGINAL &
-104cis-title product, m.p. 163-164° C., and 260 mg (32%) of the trans-title product, m.p. 152-153° C., both recrystallized from CH^Cl^/diisopropyl ether.
cis-isomer. MS (m/e) 396 (M+) , 254 , 143 , 117 and 5 91. IR (CHC1-.) 1616, 1600, 1560 cm”1. 1H-NMR(DMSO-dc,
300 MHz)delta(ppm) : 1.86 (m, 1H) , 2.8 (m, 2H) , 2.98 (m, 1H), 3.3 (m, 1H covered by H2O peak), 4.26 (bs,
OH), 4.97 (d, J=5 Hz, OCH), 5.19 (s, OCH2), 5.46 (s,
NH) , 6.38 (d, J=8 Hz, ArH), 6.75 (m, 2ArH), 7.1-7.4 (m,
6ArH), 7.6 (m, 2ArH), 7.75 (dd, J=8, 8 Hz, ArH), 7.97 (dd, J=8, 8 Hz, ArH) and 8.36 (d, J=8 Hz, ArH).
Analysis calculated for C_ _,N_O_· iH_O :
Z o z z z z z
C, 78.27? H, 5.68; N, 7.02%
Found: C, 78.35; H, 6.25; N, 6.92%.
trans-isomer. MS (m/e) 396 (M+), 254 , 143 , 117 and 91. IR (CHC13) 1619, 1601, 1561 cm”1.
1H-NMR(DMSO-dg, 300 MHz)de1ta(ppm) : 1.92 (m, 1H) , 2.31 (dd, J=12, 10 Hz, 1H), 2.67 (dd, J=15, 6 Hz, 1H), 2.78 (m, 1H), 3.09 (m, 1H), 4.18 (t, J=6 Hz, OCH, with D2O:
d, J=5.8 Hz), 5.15 (d, J=6 Hz, OH), 5.26 (s, OCH2>,
5.46 (s, NH) , 6.44 (d, J = 8 Hz, ArH), 6.76' (bd, J=3 Hz,
ArH), 6.92 (bs, ArH), 7.1-7.3 (m, 5ArH), 7.63 (dd, J=3, 8 Hz, ArH), 7.69 (d, J=8 Hz, ArH), 7.80 (dd, J=8 , 3 Hz, ArH) , 8.02 (m, 2ArH) , and 8.22 (d, J=8 Hz, ArH) .
Analysis calculated for C2gH2ON202·1K2O:
C, 73.27; H, 5.63; N, 7.02%
Found: C, 78.32 ; H, 5.80; N, 6.89%.
bad original
-105EXAMPLE 168
3-3enzylidene-6-methoxythiochroman-4-one By the method of Example 1, 39.9 g (0.206 mol) of
6-methoxythiochroman-4-one (Chem. Abstracts 66:46292η) and 21.S g (0.206 mol) of benzaldehyde gave 54.8 g (95%) of present title compound, m.p. 124-126° C.
MS (m/e) 282 (M+), 265, 253, 194, 177, 166 151,
138, 122 and 115. IR (CHC13) 1661, 1594 cm1. 1H-NMR(CDCI3) delta (ppm) : 3.85 (s, OCH-j), 4.05 (d,
J=1.5 Hz, CH2), 6.92 (dd, J=8, 2 Hz, ArH), 7.1-7.4 (m,
6ArH) , 7.63 (d, J = 2 Hz, ArH). and 7.70 (bs, vinyl H) .
EXAMPLE 169
3-Benzy1-6-methoxythiochroman-4-one By the method of Example 2, except to use methanol as solvent, 52.0 g (0.184 mol) of the title product of the preceding Example gave 39.8 g (76%) of present title compound, recrystallized from CH2Cl2/diisopropyl ether, m.p. 66-68° C.
MS (m/e) 284 (M+) , 251, 229, 193, 180, 166 150,
138 , 123 and 91. IR (CHC13) 1669 , 1600' cm1.
1H-NMR(CDCI3)delta(ppm): 2.6-3.5 (m, 5H), 3.89 (s,
OCH3), 6.97 (dd, J=8, 2 Hz, ArH), 7.08 (bs, ArH), 7.28 (m, 5ArH) and 7.62 (d, J=2 Hz, ArH).
EXAMPLE 170
3-BenzyI-6-hydroxythiochroman-4-one
By the method of Example 3, 39.8 g (0.140 mol) of the title product of the preceding Example gave 37.1 g (98%) of present title compound, m.p. 73-77° C.
MS (m/e) 270 (M+), 251, 237, 179, 166 153, 152,
124, 115 and 91. IR (KBr) 1662, 1594, 1579, 1552 cm1.
1H-NMR(CDC13 + DMSO-dg)delta(ppm) : 2.5-3.5 (m, 5H) ,
6.9-7.4 (m, 7ArH), and 7.52 (d, J=2 Hz, ArH).
ADO ft n 0 fl 3
BAD ORIGINAL
-106EXAMPLE 171
3-Benzyl-6- ( 2-quinolyl) methoxythiochroman-4-one
By the method of Example 55, 36.0 g (0.133 mol) of the title product of the preceding Example and 26.0 g (0.147 mol) of 2-chloromethylquinoline gave 20.0 g (37%) of present title compound, recrystallized from
CH2C12/diisopropyl ether, m.p. 127-130° C.
MS (m/e) 411 (M ), 320, 142, 117, 116, 115 and 91. -1 1,
IR (CHC13) 1669, 1598 cm MHz) delta(ppm) : 2.74-2.91 (m, 2H) , 2.96 (m, 1H) , 3.07 (dd, J=12, 3 Hz, 1H), 3.28 (dd, J=15, 4 Hz, 1H), 5.35 (s, OCH2), 7.06-7.32 (m, 7ArH), 7.50 (dd, J=8, 8 Hz, ArH), 7.60 (d, J=8 Hz, ArH), 7.69 (dd, J=8, 8 Hz, ArH),
7.78 (m, 2ArH), 8.05 (d, J=8 Hz, ArH), and 8.14 (d, J=8
Ή-NMR (CDC1.
300
Hz, ArH).
Analysis calculated for C^H^NC^S:
C, 75.89; H, 5.14; N, 3.40%
Found: C, 75.72; H, 5.12; N, 3.33%.
EXAMPLE 172 cis- and trans-3-Benzy1-6-(2-quinolyl)me thoxvthiochroman-4-ol
By the methods of Example 4, 2.91 g (7.08 mmol) of the title product of the preceding Example gave in order of elution 1.45 g (50%) of the cis-isomer, recrystallized from ether/hexane, m.p. 93-108° C. and 1.13 g (39%) of the trans-isomer, recrystallized from CH?Cl2/diisopropyl ether, m.p. 123° C.
c is-isomer. MS (m/e) 413 (M+) , 322 , 277 , 215 ,
182, 143, 142, 117, 116, 115 and 91. IR (KBr) 1618, 1599 , 1 56S cm'1. 1H-NMR(CDC13, 300 MHz) delta(ppm) :
2.2 (m, 1H) , 2.61 (dd, J=12, 2 Hz, 1H) , 2.72 (dd, J=14, 8 Hz, 1H), 2.92 (dd, J=14, 8 Hz, 1H), 3.10 (t, J=12 Hz, 1H), 4.46 (s, OCH), 5.26 (s, OCH2), 6.82 (dd, J=8, 2 Hz, ArH), 6.89 (s, ArH), 6.98 (d, J=8 Hz, ArH), 7.1-7.3
BAD ORIGINAL
-10 7(m, 5ArH), 7.50 (dd, J=8, 3 Hz, ArH), 7.56 (d, J = 8 Hz, ArH), 7.68 (dd, J=8, 8 Hz, ArH), 7.77 (d, J=8 Hz, ArH), 8.01 (d, J=8 Hz, Ar) and 8.12 (d, J=3 Hz, ArH).
Analysis calculated for *-26H23^°2^:
C, 75.52; H, 5.61; N, 3.39%
Found: C, 75.63; H, 5.53; N, 3.26%.
trans-isomer. MS (m/e) 413 (M+), 271 , 143, 142,
IR (KBr) 1617, 1598, 1565 cm1.
117, 116, 115 and 91.
1H-NMR(CDC13, 3C0 MHz)delta(ppm) : 2.35-2.7 (m, 4H) ,
3.34 (dd, J = 15, 3 Hz, 1H), 4.43 (d, J=4 Hz, OCH), 5.31 (s, OCH2), 6.38 (dd, J=8, 2 Hz, ArH), 7.00 (d, J=2 Hz, ArH), 7.05 (d, J = 8 Hz, ArH), 7.1-7.3 (m, 5ArH) , 7.52 (dd, J=8, 8 Hz, ArH), 7.62 (d, J=8 Hz, ArH), 7.63 (dd, J = 8, 8 Hz, ArH), 7.80 (d, J=8 Hz, ArH), 8.05 (d, J=8
Hz, ArH) and 8.16 (d, J=8 Hz, ArH).
Analysis calculated as for cis-isomer:
Found: C, 75.63; H, 5.53; N, 3.26%.
EXAMPLE 173 cis- and trans-3-Benzy1-6-(2-cuinolyl)methoxythiochroman-4-one-1-oxide and
3-5enzyl-6 - (2-quinolyl)methcxythiochroman-4-one-l,1-dioxide
AP 0 0 0 0 8 3
To a -5° to 0° C. solution of 12.0 c (29.2 mmol) of the title product of Example 171 in 100 ml CH0Cin was slowly added a solution of 3-chloroperbenzoic acid (S.02 g, 46.6 mmol) in 50 ml CH2C12. The reaction mixture was added to additional CH2C12 and saturated NaHCO3, and the organic layer separated, dried over MgSO^ and evaporated to a solid, which war. purified via column chromatography on 1 Kg of silica gel eluted with CH2C12 and ether to give in order of elution 650 mg (5%) of 1,1-dioxide, 1.16 g (9.3%) of cis-l-oxide and 6.56 g (52.6¾) of trans-l-oxide.
BAD ORIGINAL Λ
-1031,l-dioxide , recrystal/Lized from C^C^/diisopropyl ether, m.p. 177-173° C. MS (m/e) 443 (M+) , 143, 142, 117, 116, 115 and 91. IR (CHCl-J 1695, 1619,
1590 , 1571 cm-1. 1H-NMR(CDC13 , 300 MHz) delta(ppm) :
5 2.92 (dd, J=14, 10 Hz, IH), 3.4 (m, 2H) , 3. 52 (dd,
J=14, 5 Hz, IH) , 3.72 (m, IH), 5.48 (s, OCH 2), 7.17 (m
2ArH), 7.25 (m, 3ArH), 7.39 (dd, J=8, 2 Hz, ArH), 7. 58
(m, 2ArH), 7.76 (m, 2ArH), 7.83 (d, J=8 Hz , ArH), 7. 89
(d, J=8 Hz, ArH) , 8.09 (d, J-8 Hz, ArH) and 8.21 (d,
10 J=8 Hz, ArH).
Analysis calculated for Ο2θΗ2^ΝΟ^3:
C, 70.41; H, 4.77; N, 3.16%.
Found; C, 70.19; II, 4.93; N, 3.10%.
trans-l-oxide, recrystallized from CK2Cl2/diiso15 propyl ether, m.p. 176-179° C. MS (m/e) 427 (M+), 143, 142, 117, 116, 115 and 91. IR (KBr) 1684 cm-1. 1H-NMR(CDC13, 300 MHz)delta(ppm): 2.8-3.1 (m, 3H),
3.48 (dd, J=12, 2 Hz, 1H), 3.56 (dd, J=12, 3 Hz, IH), 5.45 (s, OCH2), 7.1-7.35 (m, 6ArH) , 7.42 (dd, J=8, 2
Hz, ArH), 7.54 (dd, J=8, 8 Hz, ArH), 7.60 (d, J=3 Hz, ArH), -7.73 (ddd, J=8, 8, 1.5 Hz, ArH), 7.8 (m, 2ArH), 8.07 (d, J=8 Hz, ArH) and 8.19 (d, J=8 Hz, ArH). Analysis calculated for ^26^21^03^:
C, 73.05; H, 4.95; N, 3.28%.
Found; C, 72.98; H, 5.21; N, 3.25%.
cis-l-oxide, recrystallized from CH2Cl2/diisopropyl ether, m.p. 149-151° C. MS (m/e) 427 (M+), 411, 410, 143, 142, 115, 105 and 91. IR (CHCl-J 1693, 1588, 1567 cm-1. 1H-NMR(CDC13, 300 MHz)delta(ppm): 2.8-3.1 (m, 2H), 3.35 (dd, J=12, 3 Hz, IH), 3.49 (dd, J=15, 5
Hz, IH), 4.02 (m, IH), 5.48 (s, OCH2), 7.1-7.9 (m, 12ArH), 8.08 (dd, J=8 Hz, ArH), and 8.20 (d, J=8 Hz, ArH). ·
Analysis calculated as for cis-isomer:
Found: C, 73.25; H, 5.18; N, 3.26%.
BAD ORIGINAL
-109EX AMPLE 174 trans-3-Benzyl-6-(2-quinolyl)methoxythlochroman-4-ol-l, 1-dioxide
To a 0° C. solution of 400 mg (0.903 mmol) of the 5 1,1-dioxide title product of the preceding Example in ml methanol, 2 ml tetrahydrofuran and 2 ml CHjC^ was added 25 mg (0.657 mmol) of sodium borohydride. After 5 minutes, the reaction was diluted with 2 ml water and the precipitate which formed collected by filtration.
Drying of the solid in vacuo gave 391 mg (97%) of present title compound, m.p. 190-191° C.
MS (m/e) 445 (M+), 354, 263, 143, 142, 115, 105 and 91. IR (CHCl-j) 3573, 3390, 1597, 1571 cm-1.
1H-NMR(DMSO-dg + CDCl3) delta(ppm) : 2.25 (dd, J=14, 11
Hz, 1H), 2.42 (m, 1H), 2.46 (dd, J=13, 10 Hz, 1H) , 2.8 (dd, J=12, 4 Hz, 1H) , 2.99 (dd, J=14, 5 Hz, ArH) , 4.09 (t, J=8 Hz, OCH; with D2O: d, J=9.23 Hz), 5.04 (s, OCH2) , 5.49 (d, J=8 Hz, OH), 6.69 (dd, J=8, 2 Hz, ArH), 6.75-6.95 (m, 5ArH), 7.07 (d, J=2 Hz, ArH), 7.1-7.4 (m,
4ArH), 7.46 (a, J=8 Hz, ArH), 7.63 (d, J=8 Hz, ArH) and 7.84 (d, J=8 Hz, ArH).
Analysis calculated for C26H23NO4S’^2°:
C, 69.39; H, 5.26; N, 3.11%
Found: C, 69.58; H, 5.01; N, 3.08%.
EXAMPLE 175 £-3-Benzyl-6-(2-quinolyl)methoxythiochroman-t-4-ol-t-l-oxide (For comment re nomenclature, refer to Example 99).
By the method of the preceding Example withou't isolation of the £-3-benzyl-r-4-ol isomer, 2.00 g (4.68 mmol) of trans-l-oxide title product of Example 173 was converted to 1.45 g (72%) of present title compound, m.p. 213-215° C.
*·· £ 8 0 0 0 0 dV
BAD ORIGINAL
-11010
MS (m/e) 429 (M+), 412, 312, 311, 294, 143, 142,
117, 116, 115 and 91. 1H-NMR(CDCl^, 300 MHz)delta(ppm):
2.41 (dd, J=14, 10 Hz, 1H) , 2.61 (dd, J=13, 8 Hz, ArH) ,
3.05 (m, 2H), 3.25 (dd, J=14, 5 Hz, IH), 4.25 (d, J=9
Hz, OH), 4.39 (dd, J=9, 7 Hz, OCH; with D2O: d,
J=7.26 Hz), 6.98 (dd, J=8, 2 Hz, ArH), 7.1-7.3 (m,
5ArH), 7.36 (d, J=2 Hz, ArH), 7.5-7.6 (m, 3ArH), 7.71 (ddd, J=8, 8, 1.5 Hz, ArH), 7.79 (d, J=8 Hz, ArH), 8.05 (d, J=8 Hz, ArH) and 8.15 (d, J=8 Hz, ArH).
Analysis calculated for C_ ,H~ -.NO,S :
z6 23 3
C, 72.70; H, 5.40; N, 3.26%
Found: C, 72.55; H, 5.42; N, 3.23%.
EXAMPLE 176 r-3-Benzy1-6-(2-quinolyl) methoxythio15 chroman-c- and t-4-ol-c-l-oxide (For comment re nomenclature, refer to Example 99).
To a 10° C. mixture of 600 mg (1.41 mmol) of the cis-l-oxide title product of Example 173 in 4 ml methanol, 2 mi tetrahydro furan and 4 mi CHOC1? was added 54 mg (1.42 mmol) of sodium borohydride. After 15 minutes the reaction solution was diluted with H2O and added to additional CH2Ci2 and saturated NaCi. The organic layer was separated over Na^O^ and evaporated to an oil, which was crystallized from CH2C12 and diisopropyl ether to give 554 mg (92%) of present title compound as a 2:1 mixture of title trans-4-ol-loxide: cis-4-ol-l-oxide , m.p. 133-140° C.
MS (m/e) 429 (M+) , 412 , 294 , 143 , 142 , 1 17 , 116,
115 and 91. IR (CHCl-j) 3575 , 3350 , 1597 and 1572 cm'1 . iH-NMR(CDCl3 + D2O, 300 MHz) delta (ppm) : 4.50 (d,
J=2.64 Hz, cis- (1 ,4) OCH) and 4.79 (d, J=7.85 Hz, trans- (1,4) OCH) .
BAD ORIGINAL 0
-111Analysis calculated
C, 71.95; H, 5.46; N, 3.23%
Found; C, 71.93; H, 5.11; N, 3.13%.
EXAMPLE 177
3-Benzylidene-6-methoxy-4-chromanone
By the method of Example 1, 20.0 g (0.112 mol) 6-methoxy-4-chromanone and 11.9 g (0.112 mol) benzaldehyde gave 25.1 g (84%) of present title compound, m.p. 118° C.
MS (m/e) 266 (M+) , 151, 150, 115 and 107. IR (CHC13) 1668, 1610 cm1. 1H-NMR(CDC13)delta(ppm);
3.85 (s, OCH3) , 5.29 (d, J=2 Hz, CH2), 6.86 (d, J=8 Hz,
ArH), 7.00 (d, J=2 Hz, ArH), 7.1-7.5 (m, 6ArH) and 7.83 (t, J=2 Hz, vinyl H).
Analysis calculated for C.-,Η. .0.,:
1714.3
C, 76.68; H, 5.30%.
Found: C, 76.64; H, 5.08%.
EXAMPLE 178
3-3enzy1-6-methoxy-4-chromanone
By the method of Example 2, using CH3OH as solvent, 6.85 g (25.8 mmol) of the title product of the preceding Example was converted to 6.04 g (88%) of present title compound, m.p. 71-72° C.
•or C26H23NO3S iH2O:
ΔΡ0 0 0 0 8 3
MS (m/e) (CHC13) 1682, 2.6-3,
268 (M ), 237, 177, 150, 107 and 91. IR 1617 , 1586 cm1. 1H-NMR(CDC13) delta(ppm):
(m, CH.) , 6. SO (m, 3H) , 3.83 (s, OCH-j) , 4 [a,
J=S Hz, ArH), 6.98 id, J=2 Hz, ArH) and 7.2 (m, 6ArH) .
Analysis calculated for C.-,Η,^Ο.:
i / i 0 J
C, 76.10; H, 6.01%.
Found: C, 76.31; H, 5.90%.
EXAMPLE 179
3-Benzyl-6-hydroxv-4-chromanone ·
By the method of Example 3, 5.50 g (20.5 mmol) of the title product of the preceding Example was
BAD ORIGINAL ft
-112converted to 5.11 g (98%) of present title compound, m.p. 140-143° C.
1H-NMR(CDC13)delta(ppm): 2.6-3.6 (m, 3H), 4.3 (m,
CH2), 6,35 (bs, OH), 6.82 (d, J = 8 Hz, ArH), 7.15 (dd,
J=8, 2 Hz, ArH), 7.3 (m, 5ArH) and 7.51 (d, J=2 Hz,
ArH) .
EXAMPLE 180
3-Benzvl-6-(2-quinolyl)methoxy-4-chromanone
3y the method of Example 138, 4.96 g (19.5 mmol) of title product of the preceding Example and 4.13 g (19.5 mmol) of 2-chloromethylquinoline hydrochloride gave 2.58 g (33%) of present title compound.
1H-NMR(CDC13) delta(ppm) : 2.66 (dd, J=15, 12 Hz,
1H) , 2.86 (8 line m, 1H), 3.24 (dd, J = 14, 4 Hz, 1H) ,
4.10 (dd, J=14, 10 Hz, 1H), 4.29 (dd, J=14, 10 Hz, 1H), 5.34 (s OCH2), 6.89 (d, J=8 Hz, ArH), 7.25 (m, 8ArH), 7.53 (m, 2ArH), 7.62 (d, J=8 Hz, ArH), 7.70 (dd, J=8, 8 Hz, ArH), 7.80 (d, J=8 Hz, ArH), 8.07 (d, J=8 Hz, ArH) and 8.17 (d, J=8 Hz, ArH).
EXAMPLE 181 cis- and trans-3-Benzvl-6( 2-quinolyl)methoxy-4-chromanol
By the method of Example 139, 2.58 g (6.53 mmol) of title product of the preceding Example gave in order of elution (on 150 g silica gel with 66% ether-hexane as eluant), and after recrystallizaticn from CH2Cl2/ai-
isopropyl ether, 353 mg (33%) of the cis-isomer , m.p.
107-108° C., and 710 mg (27%) of the trans-isomer, m.p.
148-149° C.
cis-iscmer. MS (m/e) 397 (M+), 261, 142, 115 and
91. IR (CHC13) 1619, 1601, 1566 cm1 1H-NMR(CDC13,
300 MHz) delta (ppm) : 1.99 (d, J=6 Hz, OH), 2.28 (m,
CH), 2.63 (dd, J=13, 7 Hz, 1H), 2.86 (dd, J=13, 7 Hz,
1H), 4.02 (d, J=9 Hz, 1H), 4.45 (dd, J=8, 4 Hz, OCH) ,
5.26 (s, OCH2), 6.75 (d, J=8 Hz, ArH) , 6.87 (m, 2ArH),
BAD ORIGINAL
-1137.25 (m, 5ArH), 7.52 (m, ArH), 7.60 (d, J=S Hz, ArH), 7.70 (dd, J=8, 8 Hz, ArH), 7.80 (d, J=8 Hz, ArH), 8.04 (d, J=3 Hz, ArH) and 8.14 (d, J=8 Hz, ArH).
Analysis calculated for C26H23N<^3:
C, 78.57; H, 5.38; N, 3.52%
Found: C, 78.61; H, 5.80; N, 3.47%.
trans-isomer. MS (m/e) 397 (M+), 255, 142, 115 and 91. IR (CHC13) 1619, 1601, 1566 cm1.
1H-NMR(CDC13, 300 MHz)delta(ppm): 2.05 (d, J=6 Hz,
OH), 2.18 (m, 1H), 2.52 (dd, J=14, 10 Hz , 1H) , 2.70
(dd, J=14, 6 Hz, 1H), 3.93 (dd, J=13, 4 Hz , 1H) , 4.16
(dd, J = 12, 2 Hz, 1H), 4.43 (bt, OCH), 5.32 (s, OCH3),
6.80 (d, J=8 Hz, ArH), 6.92 (dd, J=8, 2 Hz , ArH) , 7.00
(d, J=2 Hz, ArH), 7.1-7.3 (m, 5ArH), 7.54 (dd, J=8, 8
Hz, ArH), 7.66 (d, J=8 Hz, ArH), 7.72 (dd, J=8, 8 Hz,
ArH), 7.82 (d, J=8 Hz, ArH), 8.06 (d, J=8 Hz, ArH) and
8.18 (d, J=8 Hz, ArH).
Analysis calculated for C26H23NO3:
C, 78.57; H, 5.38; N, 3.52%.
Found: C, 73.54 ; H, 5.76;. N, 3.47%. ' .
EX-AMPLE 13 2
3-Benzyl-6-(2-pyridyl)methoxy-4-chromanone By the method of Example 138, 5.00 g (19.7 mmol) of the title product of Example 179 and 4.84 g (29.5 mmol) of 2-chloromethylpyridine hydrochloride gave 5.6 g (82%) of present title compound.
EXAMPLE 18 3 cis- and trans-3-Benzyl-6(2-pyridyl)methoxy-4-chromanol
By the method of Example 139, 5.60 g (16.2 mmol) of title product of the preceding Example gave, in order of elution (600 g silica gel, eluted with ether) and after recrystallization from ether-hexane, 3.59 g (54%) of the cis-isomer, m.p. 125° C., and 1.97 g (35%) of the trans-isomer, m.p. 126-127° C.
AP 0 0 0 0 8 3 bad ORIGINAL 3 — 114 — cis-isomer. MS (m/e) 347 (M+), 255, 209, 191,
167, 137, 117 and 91. IR (CHC1J 3653, 3340, 1595, -11 J
1574 cm . Η-NMR(CDCl^, 300 MHz)delta(ppm): 2.29 (m,
IK), 2.85 (dd, J=13, 7 Hz, 1H), 2.87 (dd, J=13, 7 Hz, 1H), 4.03 (d, J=7 Hz, CH2), 4.47 (d, J=3 Hz, OCH), 5.10 (s, OCH2), 6.75 (d, J=8 Hz, ArH), 6.83 (m, 2ArH), 7.1-7.4 (m, 6ArH), 7.47 (d, J=8 Hz, ArH), 7.69 (dd,
J=8, 8 Hz, ArH) and 8.53 (d, J=5 Hz, ArH).
Analysis calculated for C22H21NO3:
C, 7 6.06; H , 6.09; N, 4.0 3 %
Found: C, 75.87; H, 6.32; N, 4.12%.
trans-isomer. MS (m/e) 347 (M+), 255, 211, 191,
167 , 137 , 117 and 91. IR (CHCl.^) 3583, 3376 , 1595,
1573 cm-1. 1H-NMR(CDC13 , 300 MHz)de1ta(ppm) : 2,19 (m,
1H), 2.52 (dd, J=14, 9 Hz, 1H), 2.72 (dd, J=14, 7 Hz, 1H), 3.90 (dd, J=12, 6 Hz, 1H), 4.16 (dd, J=12, 2 Hz, 1H), 4.43 (d, J=5 Hz, OCH), 5.14 (s, OCH-j)', 6.78 (d,
J=8 Hz, ArH), 6.90 (dd, J=8, 2 Hz, ArH) 6.95 (d, J=2 Hz, ArH), 7.1-7.4 (m, 6ArH), 7.51 (d, J=3 Hz, ArH),
7.71 (dd, J=8, 8 Hz, ArH) and.8.54 (d, J=5 Hz, ArH).
Analysis calculated for C^H^NO^.
C, 76.06; H, 6.09; N, 4.03%
Found: C, 75.93; H, 6.31; N, 4.30%.
EXAMPLE 184 trans-3-Benzy1-6-(2-pyridyl)methoxv-4-chrcmanol Hvdrochloride
By the method of Example 118, 800 mg (2.31 mmol) of title product of the preceding Example gave 841 mg (95%) of present title compound, crystallized from ethanol-ether, m.p. 138-140° C.
MS (m/e) 347 (M+-HC1), 255, 211, 191, 137, and 91. IR (KBr) 3355, 1615, 1527, 1491 cm1. 1H-NMR(DMSO-dg, 300 MHz)delta(ppm): 2.10 (m, IK), 2.44 (dd, J=14, 8
Hz, 1H), 2.77 (dd, J=14, 6 Hz, 1H), 3.84 (dd, J=12, 6
BAD ORIGINAL ft
-115Hz, 1H) , 4.05 (dd, J=12, 2 Hz, 1H) , 4.30 (d, J=6 Hz, OCH), 5.38 (s, OCH3), 6.76 (d, J=8 Hz, ArH), 6.93 (dd,
J = 3, 2 Hz, ArH) 7.08 (d, J=2 Hz, ArH), 7.1-7.4 (m,
5ArH) , 7.82 (dd, J=8 Hz, ArH), 7.94 (d, J=8 Hz, ArH),
8.36 (dd, J=8, 8 Hz, ArH) and 8.82 (d, J=5 Hz, ArH).
Analysis calculated for C22H22ClN<^3* H20:
C, 65.75; H, 6.02; N, 3.49%
Found: C, 65.58; H, 6.14; N, 3.05%.
EXAMPLE 185
6-Hydroxy-3-[3,4-(methylenedioxy) benzylidene] -4-chromanor.e
By the method of Example 1, 5.7 g (34.8 mmol) of 6-hydroxy-4-chromanone and 5.21 g (34.S mmol) of piperonol gave 9.21 g (90%) of present title compound, tic (60% ether-hexane) Rf 0.30.
1H-NMR(DMSO-άθ)delta(ppm): 5.33 (d, J=2 Hz, CH2),
6.10 (s, OCH2O), 7.0 (m, 5ArK), 7.15 (m, ArH) and 7.61 (t, J=2 Hz, vinyl H).
EXAMPLE 136
6-Hydroxy-3-[3,4-(methylenedioxv) benzyl]-4-chromanone
By the method of Example 2, 9.00 g (30.4 mmol) of the title product of the preceding Example gave 7.15 g (79%) of present title compound, tic (60% ether-hexane)
Rf 0.38 .
1H-NMR(CDC13 + DMSO-dg)delta(ppm): 2.3-3.3 (m,
3K) , 3.9-4.5 (m, 2H) , 5.96 (s, OCK-,0) and 6.6-7.6 (m, 6ArH) .
AP 0 0 0 0 8 3 bad original
-11625
EXAMPLE 187
3-[3,4-(Methylenedioxy) benzyl]-6(2-quinolyl) methoxy-4-chromay:ne
By the method of Example 138, 6.90 g (23.1 mmol) of title product of the preceding Example and 4.96 g (23.1 mmol) of 2-chloromethylquinoline hydrochloride gave 5.40 g (53%) of present title compound, tic (10% ether-dichloromethane), Rf 0.39.
1H-NMR(DMSO-dg)delta(ppm): 2.55 (m, 1H), 3.0 (m,
2H), 4.08 (dd, J=12, 12 Hz, 1H), 4.27 (dd, J=12, 5 Hz, 1H) , 5.35 (s, OCH2) , 5.97 (s, OCH2O) , 6.62 (d, J=S Hz, ArH), 6.78 (m, 2ArH), 6.98 (d, J = 3 Kz, ArH), 7.30 (m, 2ArH), 7.60 (m, 2ArH), 7.76 (dd, J=8, 8 Hz, ArH), 7.98 (m, 2ArH) and 8.38 (d, J=8 Hz, ArH).
EXAMPLE 188 cis- and trans-3-[3,4-(Methylenedioxy) benzyl]-6-(2-quinolyl) methoxy-4-chromanol
By the method of Example 139, 3.92 g (8.92 mmol) of title product of the preceding Example gave in order of elution (on 400 g silica gel, eluted with 25% ethyl acetate-dichloromethane) and after recrystallization from dichloromethane-diisopropylether, 1.91 g (49%) of the cis-isomer, m.p. 140-143° C., and 1.16 g (30%) of the trans-isomer, m.p. 153-155° C.
cis-isomer. MS (m/e) 441 (M+) , 281 , 261 , 251 ,
160, 142, 135 and 115. IR (CKC13) 3590, 3355, 1619,
1601, 1566 cm1. 1H-NMR(CDCI3, 300 MHz)delta(ppm):
1.85 (d, J=6 Hz, OH), 2.17 (m, 1H), 2.51 (dd, J=14, 7
Hz, 1H), 2.71 (dd, J=13, 8 Hz, 1H), 3.97 (d, J=8 Kz,
2H), 4.41 (bt, OCH; with D2O: D, 0=3.37 Hz), 5.24 (s, OCH2), 5.89 (s, OCH2O), 6.65 (m, 4ArH), 6.83 (m, 2ArK), 7.48 (dd, J=8, 8 Hz, ArH), 7.57 (d, J=8 Hz, ArH), 7.67 (dd, J=8, 3 Hz, ArH) 7.76 (d, J=8 Hz, ArH), 8.00 (d,
J=8 Hz, ArH) and 8.11 (d, J=8 Hz, ArH).
BAD ORIGINAL &
-117Anaivsis calculated for “ 2/23 a
C, 73.46 ; H, 5.25; N, 3.17%
Found: C, 73.73; H, 5.15; N, 3.12%.
trans-isomer. MS (m/e) 441 (M+) , 281, 261, 251,
160, 143, 142, 135 and 115. IR (CHCi.^) 3590, 3355,
1619, 1601, 1566 cm1. 1H-NMR(CDC13, 300 MHz) delta(ppm) : 2.08 (m, 1H) , 2.40 (dd, J=15, 11 Hz, 1H) , 2.58 (dd,
J=14, 6 Hz, 1H), 3.87 (dd, J=12, 5 Hz, 1H), 4.12 (dd, J=ll, 2 Hz, 1H), 4.38 (bt, OCH; With D2O: d, J=3.96
Hz), 5.28 (s, CCH2), 5.39 (s, OCH2O), 6.54 (d, J=8 Hz, ArH), 6.62 (d, J=2 Hz, ArH), 6.67 (d, J=8 Hz, ArH),
6.75 (d, J=8 Hz, ArH), 6.89 (dd, J=8, 2 Kz, ArH), 6.95 (d, J=2 Hz, ArH), 7.50 (dd, J=8, 8 Hz, ArH), 7.62 (d,
J=8 Hz, ArH), 7.66 (dd, J=8, 8 Hz, ArH), 7.68 (d, J=3
Hz, ArH), 8.02 (d, J=8 Kz, ArH) and 8.14 (d, J=8 Hz,
ArH) .
Analysis calculated for C^H^NO^:
C, 73.46; H, 5.25; N, 3.17%
Found: C, 73.39; H, 5.08; N, 3.16%.
EXAMPLE 189
6-Hydroxy-3- ( 3 , 4-cimethoxybenzylidene) -4-chromanor.e
By the method of Example 1, 5.00 g (30.5 mmol) of 6-hydroxy-4-chromanone and 5.06 g (30.5 mmol) of veratraidehyce gave 4.29 g (45%) of present title compound, tic (66% ether-hexane) Rf 0.13.
EXAMPLE 190 trans-6-Hydroxy-3-(3,4-dimethoxybenzyl)-4-chromanol
To a 0° C. suspension of 1.20 g (33.6 mmol·) of lithium aluminum hydride in 26 ml tetrahydrofuran was added (slowly) a suspension of 4.19 g (13.4 mmcl) of title product of the preceding Example in 30 ml tetrahydrofuran. The reaction mixture was heated to reflux for 30 minutes, then cooled to 0° C. and quenched with water. The quenched reaction was
AP 0 0 0 0 8 3
BAD ORIGINAL
-118acidified with 60 mi 10% sulfuric acid and extracted with ether. The organic extract was dried over McSO, and evaporated. The crude product was purified via column chromatography on 400 g of silica gel eluted with 50% ethyl acetate/CH^Cl^ to yield (after crystallization from dichloromethane-isopropyl ether) 900 mg (21%) of present title compound, m.p. 165-166° C.
MS (m/e) 316 (M+), 152, 137, 121 and 107. IR (KBr) 1593, 1515, 1495 cm
DMSO-d-) delta (oom) : 2.05 (m, 1H) , 2.34 (dd, J = 15, 10 b
Hz, 1H), 2.59 (dd, J=15, 6 Hz, 1H), 3.45 (d,
OH), 3.76 (s, 2CCH3), 4.05 (dd, J=12, 2 Hz, (t, J=6 Hz, OCH), 6.5-6.8 (m, 6ArH) and 7.91 (s, OH). Analysis calculated for C
C, 67.86; H, 6.41%.
Found: C, 67.81; H, 6.37%.
EXAMPLE 191 -H-NMR(CDC13 + .·1/8H.0. 18205 z o=0 r. z , H) , 4.26
Vb u ι trans-3- (3 , 4-Dimet'noxybenzyl) -6(2-qu inolvl) methoxy-4-chromanol
By the method of Example 138, 700 mg (2.22 mmol) of the title product of the preceding Example and 711 mg (3.32 mmol) of 2-chloromethylquinoline hydrochloride gave 700 mg (69%) of present title compound, recrystallized from ethyl acetate-ether, m.p. 175° C.
MS (m/e) 457 (M+), 306, 261, 176, 152, 151, 144,
143 and 115. IR (KBr) 1617, 1599, 1570 cm1.
^H-NMR(DMSO-d-, 300 MHz)de1ta(oom) : 2.00 (m, IK), 2.29 o' (dd, J=14, 9 Hz, 1H), 2.61 (dd, J=14, 6, 1H), 3.70 (s, 2OCH3), 3.77 (dd, J=12, 6 Hz, 1H), 3.99 (dd, J=12, 2 Hz, 1H), 4.19 (bs, OCH), 5.28 (s, OCHo), 5.43 (bs, OK),
6.57 (d, J=8 Hz, ArH), 6.66 (d, J=8 Hz, ArH), 6.72 (bs, ArH), 6.78 (d, J=8 Hz, ArH), 6.85 (dd, J=8, 2 Hz, ArH),
SAD ORIGINAL
-1196.99 (d, J = 2 Hz , ArH) , (d, J=8 Hz, ArH), 7.74 (dd, J=8 ,
2ArH) and 8.37 (d, J=8 Hz, ArH).
Analysis calculated for C_,oH__N0c:
C, 73.51; H, 5.95; N, 3.06%.
Found: C, 73.28; H, 5.92; N, 2.92%.
EXAMPLE 192
3S,4R- and 3R,4S-3-Benzyl-6-(2-quinolyl)methoxy-4-chromanyl R-O-Acetylmandelate
By the method of Example 6, 19.97 g (50.30 mol) of the trans-title product of Example 181 and 11.71 g (60.36 mol) of (R)-(-)-O-Acetylmandelic acid gave in order of elution (2.7 kg silica gel, eluted with 10% ether-toluene) and after recrystallization from dichloromethane-ether 10.87 g (37.7%) of the 3S,4R-diastereomer, m.p. 142-145° C., and 5.97 g (20.7%) of the 3R,4S-diastereomer . The absolute configuration of these diastereoisomers was determined by X-ray crystallography.
(dd, J=8, 8 Hz, ArH), 8 Hz, ArH) , 7-.96
3S,4R-aiastereoisomer:
: & o , .6,
MS (m/e) 573 (M ), 396,
IR (CHC13) 1740, 1612
AP 0 0 0 0 8 3
380 ,
1599 cm (dd, J=12, 6 (dd, J=12, 2
J=14 Hz, 1H) , 5.63 (d, J=3 Hz, OCH) , 5.81 (s, CH) , 6.5: (d, J=2 Hz, ArH), 6.74 (d, J=8 Hz, ArH), 6.87 (dd, J=3 2 Hz, ArH), 7.1-7.45 (m, lOArH), 7.51 (dd, J=8, S Hz, ArH), 7.57 (d, J=8 Hz, ArH), 7.70 (dd, J=S, 3 Hz, ArH)
CH3CO), 2
261, 237, 142 and 91 ^-NMRiCDCl-j, 300 MHz) delta (ppm) : (m, 1H), 2.47
Hz , 1H) , Hz , 1H) ,
2.16 (s (dd, J=12, 10 Hz, 1H), 2.71 (dd, J=12, 3 Hz, 1H), 4.05 (d, J=14 Hz, 1H) , 5.11 (d, bad ORIGINAL £
-12C7.80 (d, u — 8 Hz (d, J=8 Hz, ArH c=0.0116)
Analysis calculated for C
C, 75.38; H, 5.45; N, 2.44%.
Found; C, 75.54; H, 5.47; N, 2.45%. 3R,4S-diastereoisomer:
ArH), 3.05 (d, J=8 Hz, ArH) and 8.15 [alpha];?0 = +0.69° (acei •36H31N°6:
MS (m/e) 573 (M ), 380, 288, 260, 237, 142 and 91. IR (CHC13) 1740, 1599 cm 3, 300 MHz)delta(ppm); 1.99 (m, 1H), 2.18
1,
H-NMR(CDC1
10 (s , CH3CO), 2.37 (dd,
6 Hz , IK), 3.76 (d, J
5.68 (d, J=3 Hz, OCH)
ArH) , 6.90 (d, J = 2 Hz
7.01 (d, J=8 Hz, ArH)
15 J=8, 8 Hz, ArH), 7.7
cr 8.06 (d, J=8 Hz, ArH)
(alp ha]§° = -41. 65° (
'3
J3
3»
Analysis calculated for C^H^NOg’.
C, 75.33; H, 5.45; N, 2.44%.
Found; C, 75.13; H, 5.51; N, 2.39%.
EXAMPLE 193
3S-Benzyl-6-(2-quinolyl)methoxy-4R-chromanol
By the method of Example 7, the 3S,4R-diastereomer 25 of the preceding Example gave 6.42 g (87%) of the title compound, recrvstallized from CH^^-diisopropy1 ether,
m.p. 137-138° C.
bad original
-121MS , IR and XH-NMR were identical· to those of the racemic trans-title product of Example 181, and of the
3R,4S-enantiomer of the next Examole.
[alpha]D = +21.6° (methanol, c=0.0101)
Analysis calculated for C26H23N<^3:
C, 78.57; H, 5.38; N, 3.52%.
Found: C, 78.19; H, 5.74; N, 3.50%.
EXAMPLE 194
3R-Benzyl-6-(2-quinolyl) 10 methoxy-4S-chromanol
By the method of Example 7, 5.91 g (10.3 mmol) cf the 3R,4S-diastereomer of Example 191 gave 3.76 g (92%) of present title compound, recrystallized from
CH?Cl_-diisopropyl ether, m.p. 138° C.
MS, IR and H-NMR were identical to those of the racemic trans-title product of Example 181, and of the 3S,4R-enantiomer of the preceding Example.
(alpha]2Q = -21.9° (methanol, c=0.0122)
Analysis calculated for C2gH23NO3:
C, 78.57; H, 5.38; N, 3.52%.
Found: C, 78.32; h, 5.75; N, 3.47%.
EXAMPLE 195
3-Ber.zyl-6- (2-quinolyl) methoxy-4-chromany 1 4-Piperidinobutyrate Dihydrochloride
To a 0° C. mixture of 980 mg (8.04 mmol) ·
4-(N,N-dimethylamino)pyridine, 1.25 g (6.04 mmol) of 4-piperidinobutyric acid hydrochloride and 2.00 g (5.04 mmol) of the trans -title product of Example 181 in 10 ml of dichlorom .‘thane was added 1.14 g (5.54 mmol) of dicyclohexvl carbodiimide. The resultant mixture was stirred for 15 hours at 25° C. and then filtered. The filtrate was evaporated and the residue purified via column chromatography on 150 g of silica gel eluted with 10% metnanol-dichloromethane to give an oil. This £ 8 0 0 0 0 dV
BAD ORIGINAL £ — 12 2 — oil was dissolved in ethanol and acidified with 10.1 ml of IN hydrochloric acid. The solvent was removed on a rotating evaporator and the residue crystallized from dichloromethane-diisopropyl ether to give 2.97 g (95%) of present title compound, m.p. 145-150° C.
1H-NMR(CDC13, 300 MHz)delta(ppm): 1.25 (m,), 1.8 (m),
2.22 (m), 2.38 (m), 2.47 (dd, J=14, 9 Hz, IH), 2.60 (dd, J=12, 6 Hz, IH), 2.81 (m), 2.9-3.2 (m) , 3.46 (m), 3.99 (dd, J=12, 2 Hz, IH), 4.04 (dd, J=12, 2 Hz, IH), 5.64 (d, J=3 Hz, OCH) , 5.76 (d, J=20 Hz, IH) , 5.82 (d, J=20 Hz, IH), 6.81 (d, J=8 Hz, ArH), 6.96 (m, 2ArH), 7.1-7.3 (m, 5ArH), 7.82 (dd, J=8, 8 Hz, ArH), 8.01 (dd, J=8, 8 Hz, ArH), 8.08 (d, J=8 Hz, ArH), 8.12 (d, J=8 Hz, ArH) and 8.82 (m, 2ArH).
Analysis calculated for C35H4qc12N2°4.H2O:
C, 65.52; H, 6.60; N, 4.37%.
Found: C, 65.69; H, 6.40; N, 4.37%.
EXAMPLE 196 .0
3S-Benzyl-6-(2-cuinolyl)methoxy-4Rchromanyl 4-Piperidinobutyrate
By the method of Example 195, 3.21 g (8.09 mmol) of the title product of Example 193 and 2.01 g (9.70 mmol) of 4-piperidinobutyric acid hydrochloride gave 4.45 g (88%) present title product as a solid.
MS (m/e) 550 (M+-2 HC1) , 407, 379 , 288 , 237 , 169 , 147 , 142 , 115, 98 and 91. IR (CHCl-j) 1730 , 1647 ,
1602 cm 1.
Analysis calculated for C35K40C12N2°4:
C, 67.40; H, 6.46; N, 4.49%.
Found: C, 67.56; H, 6.57; N, 4.40%.
[alphal^0 = +44.20° (methanol, c=0.0119).
BAD ORIGINAL $
EXAMPLE 197
3R-Benzyi-6-(2-quinolyl)methoxy-4Schromanyl 4-Piperidinobutyrate
By the method of Example 195, 1.38 g (4.74 mmol) of the title product of Example 194 and 1.18 g (5.68 mmol) of 4-piperidinobutyric acid hydrochloride gave 2.68 g (91%) present title product as a solid.
MS (m/e) 550 (M+-2 HCl), 407, 379, 288, 237, 170, 169, 147 , 142, 115, 98 and 91. IR (CHC13) 1730, 1646, 1602 cm-1.
Analysis calculated C, 67.40; H
Found: C,. 67.58; H [alpha]2° = -43.38° for c35H40C12N2O4: 6.46; N, 4.49%.
6.55; N, 4.41%.
(methanol, c=0.0114). EXAMPLE 198 trans-3-Benzyl-6-(2-quinolyl)methoxy4-chromanvl Hemisuccinate
To a solution of 1.00 g (2.52 mmol) of the transtitle product of Example 181 in 8 ml pyridine was added 277 mg (27.7 mmol) of succinic anhydride and the reaction heated at 80° C. for 12 hours. The reaction was evaporated in vacuo to an oil which crystallized upon addition of ether. Recrystallization from dichloromethane-diisopropyl ether gave 912 mg (73%) of present title product, m.p. 175-176° C.
MS (m/e) 497 <M+) , 396, 379, 362, 261, 237, 142 £ 8 0 00 OdV
1.
H-NMR(CDC13, 300 and 91. IR (K3r) 1731, 1703 cm MHz)delta(ppm): 2.30 (m, In,, 2.4-2.8 (m, 6K), 3.83 (dd, J=12, 6 Hz, 1H), 4.00 (dd, J = 12, 2 Hz, 1H) , 5.29
Γ=15 Hz, 1H) , 5.75 (d, J=6 Hz, CCH), 6.70 (d, J=3 Hz, ArH), 6.84 (dd, J=8, 2 Hz, ArH), 6.94 (d, J=2 Hz, ArH), 7.1-7.3 (m, 5ArH), 7.52 (d , J=15 Hz , 1H) , (d,
BAD ORIGINAL
-12 410 (dd, J=8, 3 Hz, ArH), 7.70 (m, 2ArH), 7.79 (d, J=3 Hz,
ArH), 8.15 (d, J=3 Hz, ArH) and 8.21 (d, J=3 Hz, ArH).
Analysis calculated for C, ..H-.-.NO, :
JU J / 0
C, 72.42; H, 5.47; N, 2.82%.
Found: C, 72.16; K, 5.43; N, 2.70%.
EXAMPLE 199 trans-3-Benzy1-6-(2-quinoly1)methoxy-4chromanyl Hemisuccinate Ester, Sodium Salt
To a solution of 300 mg (0.'604 mmol) of the title product of the preceding Example in 50 mi ethanol was added 0.604 ml of IN sodium hydroxide. The reaction solution was evaporated in vacuo and the residue triturated with ether to give a quantitative yield of present title product as a solid.
EXAMPLE 200 trans-3-Benzy1-6- (2-quinolyl)methoxy-4-chromanyl Hemisuccinate Ester, Ethanodamine Salt *Τ2· ‘ I o
Ό
J3
To a solution of 300 mg (0.604 mmol) of the title product of Example 198 in 50 ml dichloromethane was added 36.8 mg (0.604 mmol) of ethanolamine. The reaction solution was evaporated in vacuo and the residue triturated with ether to give a quantitative yield of present title product as a solid.
EXAMPLE 201
6-Met’noxv-3- ( 2-pyridvl) methylene-4-chromanone
By the method of Example 1, 20.0 g (0.112 mol) of 6-raethoxy-4-chromanone and 18.0 g (0.168 mol) of 2-pyridinecarbaldehyde gave 17.5 g (60%) of present title product, m.p. 109-111° C.
MS (m/e) 267 (M+) , and 117. IR (CHCip 1663,
1611 , 1586 , 1564 cm-1. 1H-NMR (CDCl-j, 300 MHz) delta (ppm) : 3.80 (s, GCH-j) , 5.83 (d, J=2 Hz, CH2), 6.89 (d, J=S Hz, ArH), 7.17 (dd, J=8, 2 Hz, ArH), 7.2 (m, ArH), 7.39 (d, J=2 Hz, ArH), 7.46 (d, J=8 Hz, ArH), 7.70 (m, 2ArH) and 8.67 (d, J=2 Hz, vinyl H).
tAD ORIGINAL
-125Analysis calculated C, 71.90; H,
Found: C, 71.98; H,
°r C16HnNO3:
4.90; N, 5.24%
4.90; N, 5.22%
EXAMPLE 202
6-Methoxv-3-(2-pyridyImethyl)-4-chromanone
By the method of Example 2, 154 g (0.577 mol) of title product of the preceding Example gave 128 g (82%) of present title product, m.p. 112-114° C.
MS (m/e) 269 (M+) , 254, 177, 118, 107 and 93. IR (KBR) 1684, 1641, 1620, 1588, 1565 cm’1. 1H-NMR(CDC13, 300 MHz)delta(ppm): 2.82 (dd, J=14, 9 Hz, 1H), 3.29 (m, 1H) , 3.39 (dd, J = 14, 4 Hz, IK), 3.74 (s, OCH-j),
4.17 (dd, J=10, 9 Hz, IK) , 4.45 (dd, J=ll, 5 Hz, 1H) , 6.84 (d, J=8 Hz, ArH), 7.02 (dd, J=8, 2 Hz, ArH), 7.03 (dd, J=8 , 8 Hz, ArH), 7.18 (dd, J=8, 2 Hz), 7.27 (d,
J=2 Hz, ArH), 7.56 (ddd, J=8, 8, 2 Hz, ArH) and 8.47 (d, J=5 Hz, ArH).
Analysis calculated for ^ιθΗ^ΝΟ^:
C, 71.13; H, 5.57; N, 5.12%.
Found: C, 71.36; H, 5.61; N, 5.12%.
EXAMPLE 203
6-Hydrc:<y-3- ( 2-pyridy Imethyl) -4-chromanone
By the method of Example 3, 128 g (0.474 mol) of the title product of the preceding Example gave 104 g (86%) of present title product, crystallized from ethyl· acetate, m.p. 150-151° C.
MS (m/e) 255 (M+) , 163, 137, 118, 117 and 93. IR (KBR) 1691, 1645, 1616, 1599, 1566 cm’1.
1H-NMR(DMSO-d,)delta(oom): 2.81 (dd, J=16, 10 Hz, 1H), o
3.2-3.3 (m, 2H and H2<D) , 4.20 (t, J=12 Hz, 1H) , 4.36 (dd, J=12, 4 Hz, 1H), 6.S8 (d, J=3 Kz, ArH), 7.01 (dd, J=8, 2 Hz, ArH), 7.10 (d, J=2 Hz, ArH)', 7.22 (dd, J=8,
Hz, ArH), 7.31 (d, J=8 Hz, ArH), 7.72 (dd, J=8, 8 Hz, ArH), 8.47 (d, J=5 Hz, ArH) and 9.50 (bs, OH).
AP 0 0 0 0 8 3 . BAD ORIGINAL
-126J3
EXAMPLE 204 cis- and trans-6-Hydroxy-3(2-pyridvImethyl)-4-chrcmanol
By the method of Example 4, 11.5 g (45.1 mmol) of 5 the title product of the preceding Example gave a crude mixture of isomers. This mixture was purified and the isomers separated via column chromatography on 830 g of silica gel eluted with 10% isopropanol-60% ethyl acetate-30% dichloromethane chromatography to give, in order of elution, 4.27 g (31%) or cis-isomer, m.p. 153-155° C. and 5.50 g (40%) of trans-isomer, m.p. 146-147° C.
cis-isomer. MS (m/e) 257 (M+) , 240 , 147 , 1 18 and 93. IR (KBr) 1617, 1599, 1569 cm1.
1H-NMR(DMSO-dg)delta(ppm) : 2.41 (m, 1H) , 2.68 (m, 1H) ,
2.96 (dd, J=14, 6 Hz, 1H), 3.91 (m, 2H), 4.35 (bs,
OCH), 5.36 (d, J=7 Hz, OH), 6.6 (m, 3ArH), 7.22 (m, ArH), 7.31 (d, J=8 Hz, ArH), 7.72 (dd, J=8, 8 Hz, ArH), 8.48 (bs, ArH) and 8.81 (s, OH).
Analysis calculated for ^^Η^ΝΟβ1
C, 70.02; H, 5.88; N, 5.44%.
Found: C, 69.86; H, 5.82; N, 5.33%.
trans-isomer. MS (m/e) 257 (M+) , 240 , 113 and 93.
IR (KBr) 1613, 1595, 1570 cm1.
1H-NMR(DMSO-άθ)delta(ppm): 2.32 (m, 1H) , 2.61 (dd,
J=13, 8 Hz, 1H), 2.83 (dd, J=13 , 5 Hz, 1H), 3.82 (dd,
J=12, 6 Hz , 1H), 4.07 (dd, J=12, 2 Hz , 1H) , 4.25 (t,
J=6 Hz, OCH; with D2O: d, J=6 Hz), 5.47 (d, J=6 Hz ,
CH), 6.58 (bs, 2ArK), 7.75 (bs, ArH), 7.24 (d, J=8 Hz, 30 ArH), 7.72 (dd, J=8 , 8 Hz, ArH), 3.51 (d, J=4 Hz, ArH) and 8.84 (s, OH).
Analysis calculated for C^H., ^NO^· 1/8H2O:
C, 69.42; H, 5.92; N, 5.40%.
Found: C, 69.61; H, 5.86; N, 5.35%.
BAD ORIGii.
-127ΞΧ AMPLE 20 5 cis-3-(2-?yridyImethy1)-6( 2-cuinolvl)methoxv-4-chromanol
3y the method of Example 5, 5.00 g (19.5 mmol) of the cis-title product of the preceding Example and 3.54 g (20.0 mmol) of 2-chloromethylquinoline gave 4.41 g (57%) of present title product, recrystallized from CH2Cl2-diisopropyl ether, m.p. 115-118° C.
MS (m/e) 398 (M+), 306, 288, 256, 142, 118 and 93. IR (KBr)· 1618 , 1594 , 1566 cm1. 1H-NMR (CDCl-j 300,
MKz)delta(ppm) : 2.38 (m, 1H) , 2.84 (dd, J=15, 5 Hz,
IK), 3.00 (dd, J=12, 11 Hz, 1H), 4.07 (m, 2H), 4.38 (d, J=3 Hz, OCH, with ΰ>20: d, J=3.63 Hz), 5.32 (s, OCHp , 5.38 (bs, OH), 6.78 (d, J=3 Hz, ArH), 6.88 (dd, J=8, 2 Hz, ArH), 7.01 (d, J=2 Hz, ArH), 7.2 (m, 3H), 7.52 (dd, J=8, 8 Hz, ArH), 7.65 (m, 3ArH), 7.81 (d, J=8 Hz, ArH), 8.05 (d, J=8 Hz, ArH), 8.16 (d, J=8 Hz, ArH) and 8.54 (d, J=5 Hz, ArH, .
Analysis calculated for ^25Η22Ν2θ3:
C, 75.36; H, 5.56; N, 7.03%.
Pound: C, 75.30; H, 5.52; N, 6.98%.
EXAMPLE 206
AP 0 0 0 0 8 3 trans-3-(2-PyridyImethy1)-6(2-quinolyl)methoxv-4-chromanone
By the method of Example 5, 4.00 g (15.6 mmol) of the trans-title product of Example 204 and 2.89 g (16.3 mmol) of 2-chloromethylquinoline gave 3.80 g (61%) of present title product, also crystallized from CH2C12~ diisopropyl ether, m.p. MS (m/e) 306, 283,
1658, 1619, 1589 cm1.
121-123° C.
256, 144, 113 and 93. IR (KBr) 1K-NMR(CDC13 + D2O, 300
MHz)delta(ppm) : 2.27 (m, 1H) , IK), 2.94 (dd, J=13, 7 Hz, 1H) 1H), 4.20 (dd, J=12, 2 Hz, 1H)
2.75 (dd, J=13, 6 Hz, 3.89 (dd,·J=12, 6 Hz, 4.56 (d, J=6.26 Hz,
BAD ORIGINAL &
-123OCH), 5.30 (s, CCH2), 6.74 (d, J=8 K:, ArH), 6.87 (dd, J=8, 2 Hz, ArH), 7.12 (m, 3ArH), 7.5-7.9 (m, 5ArH), 8.45 (d, J=3 Hz, ArH), 3.15 (d, J=S Hz, ArH) and 8.44 (d, J = 5 Hz, ArH) .
Analysis calculated for C25H22N2^3:
C, 75.36; H, 5.56; N, 7.03%.
Found: C, 75.11; H, 5.64; N, 6.95%.
trans-isomer. MS (m/e) 348 (M+), 331, 256, 238,
118 and 93. IR (CHC13) 3200, 1595, 1571 cm1.
1H-NMR(CDC13, 3C0 MHz, delta (ppm) : 2.47 (m, 1H) , 2.79 (dd, J=15, 6 Hz, 1H) , 2.95 (dd, J=13, 6 Hz, 1H) , 3.92 (dd, J=ll, 9 Hz, 1H) , 4.21 (dd, J=ll, 3 Hz, 1H) , 4.59
(d, J=6 Hz, OCH), 4.98 (bs, OH) , 5. 14 (s, OCH2), 6.73
(d, J=8 Hz, ArH), 6.82 (dd, J = 8 , 2 Hz, ArH), 7.15 (m,
15 4ArH), 7.49 (d, J=8 Hz, ArH) , 7. 60 (ddd, J=8, 8, 2 Hz,
ArH), 7.68 (ddd, J=8, 8, 2 Hz, ArH), 8.48 (d, J=6 Hz, ArH) and 8.54 (d, J=6 Hz, ArH).
Analysis calculated for C21K20N2^3:
C, 72.40; H, 5.79; N, 8.04%.
20 Found: C, 72.41; H, 5.52; N, 8.05%.
EXAMPLE 207
cis- and trans-6-(2-pyridyl)methoxy-
3-(2-pyridylmethvl) -4-chromanol
By the method of Example 78, 10.0 g (38.9 mmol) of 25 a mixture of the title products of Example 204 and
5.08 g (39.9 mmol) of 2-picolyl chloride gave, in order of elution (360 g silica gel eluted with 50% acetonedichloromethane as eluant), 3.30 g (24%) of cis-isomer, m.p. 85-95° C., and 5.69 g (42%) of trans-isomer, m.p.
103-104° C., both recrystallized from CH2Cl2-diisoprcpyl ether .
cis-isomer. MS (m/e) 348 (M+), 256, 238, 119,
118, 93 and 92. IR (CHCl-j) 3262 , 1595 , 1571 cm1.
1H-NMR(CDC13 , 300 MHz)delta(ppm) : 2.37 (m, 1H) , 2.33
BAD ORIGINAL ft
-129(dd, J=12, 5, In), 2.99 (dd, J=12, 11 Kz, IK), 4.05 (m,
2H), 4.38 (d, J=4 Hz, OCH), 5.11 (s, OCH2), 5.36 (bs,
OH), 6.73 (d, J=8 Kz, ArH), 6.82 (dd, J=8, 2 Hz, ArH),
6.93 (d, J=2 Hz, ArH), 7.2 (m, 3ArH), 7.46 (d, J=8 Hz,
ArH), 7.63 (m, 2ArH) and 8.52 (m, 2ArH).
Analysis calculated for C21H20N2°3*3/4H2O:
C, 69.69; H, 5.99; N, 7.74%.
Found: C, 69.89; H, 5.69; N, 7.88%.
EXAMPLE 208
0R,4_S- and 3J3,4R-3-(2-Pyridylmethyl)-6(2-quinolyl)methoxy-4-chromanyl R-O-Acetylmandelate
By the method of Example 192, 4.78 g (12.0 mmol) of the title product of Example 206 and 3.20 g (16.5 mmol) of (R)- (-)-O-acetylmandelic acid gave in order of elution (from 1.2 kg silica gel eluted with 33% ethyl acetate-dichloromethane) and after crystallization from CH2Cl2-diisopropyl ether, 980 mg (14%) of 3R,4S-diastereomer, m.p. 97-102° C., and 1.64 g (24%) of 3S^4R-diastereomer, m.p. 109-110° C.
3R,4£-diastereomer. ^K-NMR(CDCl^, 300
MHz)delta(ppm): 2.18 (s, CH^CO), 2.33 (m, 1H), 2.64 (m, 2H) , 3.85 (m, 2.H) , 5.28 (s, OCHp, 5.72 (d, J=4 Hz, OCH), 5.87 (s, CH), 6.78 (d, J=8 Hz, ArH), 6.9 (m,
3ArH), 7.07 (dd, J=3, 8 Hz, ArH), 7.35 (m, 6ArH), 7.51 (dd, J=8 , 8 Hz, ArH), 7.68 (m, 2ArH), 7.80 (d, J=8 Hz, ArH), 8.05 (d, J=8 Hz, ArH), 8.18 (d, J=8 Hz, ArH) and 8.48 (d, J=5 Hz, ArH) .
3S,4R-diastereomer. ^H-NMR(CDCl^ 300,
MHz) delta (ppm) : 2.17 (s, CH^CO) , 2.65 (bs, OH), 2.75 (dd, J=9, 9 Hz, 1H) , 2.87 (dd, J=12, 6 Hz, 1H) , 3.97 (dd, J=12, 3 Hz, 1H), 5.04 (d, J=14 Kz, 1H), 5.11 (d,
J=14 Hz, 1H), 5.71 (d, J=4 Hz, OCH), 5.85 (s, CH), 6.55 (d, J=2 Hz, ArH), 6.73 (d, J=8 Hz, ArH), 6.87 (dd, J=8,
BAD ORIGINAL
AP 0 0 0 0 8 3
-1302 Hz, ArH), 7.09 (m, 2ArH), 7.25 (m, 3ArH), 7.38 (m, 2ArH) , 7.55 (m, 3ArH) , 7.71 (dd, J=8 , 8 Hz, ArH), 7.31 (d, J=S Hz, ArH), 8.07 (c, J=8 Hz, ArH), 8.17 (d, J=8
Hz, ArH) and S.52 (d, J=4 Hz, ArH).
EXAMPLE 209
3R-(2-Pyridylmethyl)-6(2-qu7nolyl)methoxy-4S-chromanol
By the method of Example 7, 949 mg (1.64 mmol) of
4S, 3R-dias tereomer of the preceding Example gave 47 0 mg (72%) of present title product, recrystallized from
CH2Cl2-diiscpropyl ether, m.p. 142-143° C.
MS, IR and ^H-NMR are identical to those of the racemic trans-product of Example 206.
Analysis calculated for ε25Η22Ν2θ3*
C, 74.52; H, 5.63; N, 6.95%
Found: C, 74.68; H, 5.54; N, 6.96%.
[alpha]2° = -18.51’ (methanol, 0.01345).
EXAMPLE 210
3S_- (2-Pyridylmethyl) - 620 (2-quinolyl)methoxy-4R-chromanol ,α
3y the method of Example 7, 1.60 g (2.78 mmol) of 3S_, 4R-dias tereomer of Example 208 gave 900 mg (82%) of present title product, recrystallized from CH2Cl2~diisopropyl ether, m.p. 142-143° C.
MS, IR and ^H-NMR are identical to those of the racemic trans-product cf Example 206.
Analysis calculated for C^H-^N^Oy iH20:
C, 74.52; H, 5.63; N, 6.95%
Found: C, 74.71; H, 5.58; N, 6.98%.
Γ) [alphaK = +17.74° (methanol, 0.0155).
Bad original
-131EXAMPLS 211 cis-3-(3-?yridylmethy1)-6-(2-quinolyl)methoxy-4-chromanol Dihydrochloride
By the method of Example 184, cis-3-(2-pyridylmethyl)-6-(2-quinolyl)methoxy-4-chromanol (1.00 g, 2.51 mmol) was converted to 830 mg (70%) of the title dihydrochloride salt, crystallized from a mixture of ethanol, ether and water, m.p. 110” C. (dec.).
Analysis calculated for C25H24C12N2°3’2H2O:
C, 59.18; H, 5.56; N, 5.52%
Found: C, 59.04; H, 5.32; N, 5.44%.
EXAMPLE 212 trans-3-(3-Pyridylmethyl)-6-(2-quinolyl)methoxy-4-chromanol Dihydrochloride
By the method of Example 184, trans-title product of Example 4 (560 mg, 1.41 mmol) was converted to 572 mg (87%) of the title dihydrochloride, crystallized from acetone, ethanol and ether, m.p. 197° C.
Analysis calculated for C25H24C12N2°3’^H20:
C, 63.10; H, 5.19; N, 5.89%
Found: C, 63.24; H, 5.16; N, 5.85%.
EXAMPLE 213
3-[4-(Ethoxycarbonyl and methoxycarbonyl)-2-pyridyl]methylene-6-(2-quinolyl) methoxy-4-chromanone
By the method of Example 1, 12.1 g (39.7 mmol) of the title product of Example 55 and 7.10 g (39.7 mmol) of 4-carboethoxy-2-pyridinecarbaldehyde gave 11.8 g (64%) of present title products, a mixture of methyl and ethyl esters.
bad original
APO00083 ,21H-NMR (CBC1, , 300 MHz) ce Ita (ppm) : 1.40 (t, J = 6
Hz, C H ) , 3.95 (s, CC.-.J , 4.39 (g, J=6, OCH^ of ethyl ester), 5.36 (s, OCHJ , 5.31 (d, J=2 Hz, CHp , 6.91 (d, J=8 Hz, ArH), 2.2 (m, 2ArH), 7.4-7.8 (m, 5ArH), 7.98 (bs, vinyl H), 8.05 (d, J=8 Hz, ArH), 8.15 (d, J=8 Hz, ArH) and 8.79 (d, J=5 Hz, ArH).
EXAMPLE 214
3-(4-(Ethoxycarbonyl and methoxycarbonyl)-2pyridyl]methyl-6-(2-guinolyl·)methoxychromanone
By the method of Example 2, 11.8 g (25.3 mmol) of the title product of the preceding Example in tetrahydrofuran gave 11.7 g (99%) of present title products as a mixture of ethyl and methyl esters.
1K-NMR(CDC13 , 300 MHz)delta(ppm) : 1.35 (t, J=6 σ>
a ο
Ο
Λ
15 Hz, CH ), 2.87 (dd, J=15, 10 Hz, IH) , 3.31 (m, IH),
3.46 (m, IH) , 3.89 (s, OCHJ , 4.16 (t , J=ll Hz, IH),
4.34 (g, J=6 Hz, OCH2 of ester), 4.43 (dd, J=ll, 5 Hz
IH) , 5.29 (s, OCH2) , 6.85 (d, J = 8 Hz , ArH), 7.15 (m,
ArH), 7.4-7.8 (m, 7ArH), 8.01 (d, J = 8 Hz , ArH) , 8.11
20 (d, J=8 Hz, ArH) and 8.59 (d, J=5 Hz, ArH) .
EXAMPLE 215
cis- and trans-3 - (4-(Hydroxymethyl)-2-pyridyl]methvl-6-(2-quinolyl)methoxy-4-chromanol
By the method of Example 190, using sufficient
LiAlH^ to reduce both the kerone and ester groups,
11.7 g (25.0 mmol) of the title product of the preceding Example gave in order of elution (using 650 g silica gel eluted with acetone) 1.98 g (19%) of cis-title product as a glass and 2.04 g (20%) of
3G trans-title product, crystallized from ethyl acetate, m.p. 147-149° C.
BAD ORIGINAL ft
-133cis-isomer. MS (m/e) 306 (M+) , 236, 14S, 123 and 115. IR (KBr) 1608 , 1561 cm1. 1H-NMR (CDCl-j, 300 MHz)delta(pern): 2.26 (m, 1H), 2.71 (dd, J=14, 6 Hz,
1H), 2.87 (dd, J=14, 10 Hz, 1H), 3.95 (m, 2H), 4.25 (d, J=4 Hz, OCH), 4.62 (s, OCH^, 5.18 (s, OCH2), 6.65 (d, J=8 Hz, ArH), 6.77 (dd, J=8, 2 Hz, ArH), 6.94 (d, J=2 Hz, ArH), 7.06 (d, J=6 Hz, ArH), 7.16 (d, J=8 Hz, ArH), 7.44 (dd, J=8, 8 Hz, ArH), 7.54 (d, J=8 Hz, ArH), 7.62 (ddd, J=8, 8, 1.5 Hz, ArH), 7.72 (d, J=8 Hz, ArH), 7.96 (d, J=8 Hz, ArH), 8.07 (d, J=8 Hz, ArH) and 8.32 (d,
J=8 Hz, ArH) .
Analysis calculated for C^H^N^O^· 3/4H2O: C, 70.65; H, 5,
Found: C, 70.49; H, 5, trans-isomer. MS
81; N, 6.34%.
80; N, 5.98%.
(m/e) 306 (M+), 148, 123, 115 and 94. IR (KBr) 1602, 1557 cm ‘H-NMR(CDC13, 300
MHz)delta(ppm): 2.39 (m, 1H), 2.72 (dd, J=15, 6 Hz,
1H), 2.88 (dd, J=14, 7 Hz , 1H), 3.85 (dd, J=10, 8 Hz,
1H), 4.14 (dd, J=12, 2 Hz , 1H), 4.51 (d, J=7 Hz, OCH),
4.66 (s, OCH2), 5.24 (s , OCH2), 6.67 (d, J=8 Hz, ArH),
6.80 (dd, J=8, 2 Hz, ArH) , 7.05 (m, 3ArH), 7.47 (dd,
J=8, 8 Hz, ArH) , 7.61 (d, J=8 Hz, ArH), 7.66 (dd, J=8,
8 Hz, ArH), 7.76 (d, J=8 Hz, ArH), 3 .CO (d, J=8 Hz,
ArH), 8.12 (d, J=8 Hz , ArH) and 8.33 (d, J=8 Hz, ArH) .
Analysis calculated for C^H^I^O^· H2O:
c, 69.94; H, 5.87; N, 6.27%.
Found: C, 70.21; H, 5.49; N, 6.23%.
EXAMPLE 216
APO 00 0 8 3
3-Benzy1-6-(6 - fluoro-2-quinolyl) methoxy-4-chromanone
By the method of Example 55, 1.00 g (3.94 mmol) of the title product of Example 179 and 347 mg (4.33 mmol) of 6-fluoro-2-chloromethylquinoline gave 1.38 g (85%) of present title product, recrystallized from CH2C12~ diisopropyl ether, m.p. 142-143.5° C.
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( u. I d- , ‘ . (*'. I f *.<.· 'J f a. ‘i , Ό . . 1-. — ( C . 1 X - , · J o x, / , 1C . x , x υ u ? , i ; ύ j , * x 5 t cm . .: -'.,Μ.4 (C5C i . , 3 u x .Ί L L X ) XX '— x X. U . χ '' , * χ · U x ( 'X X— , xJ — X X , x X ,1 X , x ,1 ) , X a Ο 'X (3-line m, 1 H) , 3.35 (dd, J=14, 4 Hz, IK) , 4.11 (dd,
J=ll, 8 Hz, id), 4.31 (dd, J=10, 4 Hz, 1H), 5.33 (s,
OCH2) , 6.91 (d, J=8 Hz, ArH), 7.25 (m, 6ArH.) , 7.45 (m,
3ArH), 7.63 (d, J=8 Hz, ArH), 8.06 (dd, J=12, 8 Hz,
ArH) and S.12 (d, J=8 Hz, ArH).
Andlv3i3 CtixCUTLxcd for C.xH.xFhO-,:
2o 20 3 x ounc
C, u ,
3.39’. j Η , j. 4 4 5.
tra n:;-j-3er.
rl-6- (6-fluoro2-quinolyl) -4 -chromano 1
By the method of Example 4, 1.33 g (3.22 mmol·) of the title product of the preceding Example gave, in order of elution (130 g silica gel eluted with 10% ether-dichloromcthxine) , and following recrystallization from dichloromethane-diisopropyl ether, 691 mg (52%) of ois-1i11e product, m.p. 145-147° C. and 444 mg (33%) of
i. x- - 4. 4 4- ’ - --z ' ,·<->- — .-, i c ·’_· :;o π .-. .. -- ; I L. “ -. u. W / ... . J . 1 u -. — x υ J k, .
: y Λ ? ί η
6-Denzyloxy-3 - (1-imicazoly1)me thy1-4-^hromanou'
A solution of 3.7 g of 6-benzvloxy-3-methylene4 — cnx omanone ana a . / g of imidazole m 50 cc of DM.· was Λcu tlcG 3 Ί ύ U ° C . 10 Ί 1.5 hCUl ο , Tii*2 1C 1C11Cn Vu 3 £ * _C -·<
»’χ_Γ'. OCullC 1.1 Ίθ HC cXtloCltiC WltLO
LO COOl· , e n i y ΐ ο c o x u f . over Ha.SO, and cv w *i i c γϊ w ci b ο οι r — — o cl· to give 3 g of title product, m.p. lie—l±0° C. M ;G <.O C IVtj n t.
r G C111 crude prodoorecrystallization from CHOC1O/:
.eel :oi x xl io η i. ’ -j
0.: 334.1317; found: 334.131'
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-135ΞΧ AMPLE 219
6-Hydroxy-3-(1-imidazolyl)methyl-4-chromanone
By the method of Example 2, 3 g of the title product of the preceding Example was converted to 2 g of present title product, tic (9:1 CH2C12zCH^OH)
Rf 0.5.
EXAMPLE 220 cis- and trans-6-Hydroxy-31-imidazolyl)methyl-4-chromanol
By the method of Example 39, 2 g of the title product of the preceding Example was converted to a mixture of title products, 1.8 g, tic (9:1 CH2C12:CH^OH) Rf 0.15 (cis-isomer) and 0.17 (trans-isomer) .
EXAMPLE 221 cis- and trans-3-(1-Imidazolyl)methy1-6(2-quinolyl)methoxy-4-chromanol
By the method of Example 13, 1.8 g of a mixture of cis- and trans-title products of the preceding Example were converted to a mixture of present cis- and transproducts which were separated on silica gei eluting with CH2Ci2/MeOH. Less polar cis-isomer (680 mg) was obtained; it was recrystallized from CH,OH/ethyl acetate J to give 500 mg of pure cis-title product, m.p. 163° C.
MS calculated for 387.1610 ; found:
387.1613.
More polar trans-isomer (720 mg) was recrystallized from tetrahydrofuran and ethyl acetate to give 440 mg of pure trans-title product, m.p. 142-144° C.
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-13!
Ο
Ο
C5 ja
EXAMPLE 222
5-3enzyioxy-3-(3-methoxycarbonyl) _benzylidene)-4-chromanene_
3v the method of Example 1, 6-be.nzyloxy-4-ohro5 manone (2.5 g, 0.0098 mol) was converted to present title product, 5.76 g, as a gum, tic (9:1 CH-C1-,: hexane) Rf 0.5.
EXAMPLE 223
6-Hydroxy-3-(3-methoxy10 carbonyl)benzyl-4-chromanone
Title product of the preceding Example (5.74 g) in
167 ml of tetrahydrofuran and 83 ml of ethyl acetate was hydrogenated at 50 psig for 24 hours over 2.5 g of 10% Pd/C by which time tic (9:1 CH-^C^ : ethyl acetate) indicated complete conversion to the desired product.
The catalyst was recovered by filtration over diatomaceous earth and the filtrate stripped to dryness to yield present title product as a yellow gum, 3.0 g, tic (9:1 : hexane) Rf 0.07.
EXAMPLE 224 cis- and trans-3-(3-Methoxycarbonyl)benzyl-4 , 6-chromar.diol
Title product of the preceding Example (4'. 38 g, 0.014 mol) and Νβ3Η^ (0.563 g, 0.015 mol) were combined in 65 ml CH^OK and stirred for 30 minutes. Silica gel was then added and the mixture evaporated to dryness, charged onto a 25 cm x 10 cm silica gel column, and mixed title products eluted with 2500 ml of 49:1 CH2CI2: isopropanol to yield, after stripping, the fractions, 2.2 g of present mixed title products, tic (29:1 CH2Cl2: i-soProPano-) Rf 0.31 (cis-isomer) and 0.28 (trans-isomer).
BAD ORIGINAL
-139Method Β
To a 5° C. mixture of 22.2 g (55.7 mmol) of transtitle product of Example 5 in 75 ml of water was added 5.9 ml (111 mmol) of concentrated sulfuric acid. To this solution was added 300 ml acetone and then 79.6 ml (55.7 mmol) of 0.7M Jones Reagent was rapidly added.
The resultant mixture was stirred 1 hour at 25° C. and then added to saturated sodium bicarbonate (300 ml).
The quenched reaction mixture was extracted twice with 150 ml ethyl acetate and once with 150 ml dichloromethane. The combined organic extract was dried over magnesium sulfate and evaporated to a solid. Purification via column chromatography on silica gel eluted with 9 2:3:390:5:5 dichloromethane: isopropanol: ethyl acetate gave 18.5 g (84%) and recrystallization from ethyl acetatehexane gave product identical with that of present Method A.
This method applied to the cis-title product of Example 5 produces the same product. Applied to the product of Example 7, 3S-(3-pyridyl)methyl-6-(2-quinolyl) metho.xy-4-chromanone is produced. Applied to the product of Example 8, 3R-(3-pyridyl)methyl-6-(2-quinolyl) methoxy-4-chromanone is produced.
EXAMPLE 230 cis-3-(3-Pyridy1)methyl-6(2-quinolyl) methoxy-4-chromar.ol
By the method of Example 4A, 5.00 g (12.6 mmol) of the title product of the preceding Example gave 3.75 g (74%) of present title product (after crystallization from chloroform-diisopropyl ether) identical with the same product produced according to the method of Example 5A.
AP 0 0 0 0 8 3
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EX/C-IPLE 231 f r ?.ns - 3 -3en z y 1- 6 - (6-chloro2-pyridyl)methoxy-4-chromanol
3y the method of Example 5, 0.50 g (1.96 mmol) of trans-3-benzyl-4,6-chromandiol and 445 mg (2.16 mmol) of 2-chloro-6-(bromomethyl)pyridine were converted to present title product purified by recrystallization from CH2Cl2/hexane, to yield 0.50 g (67%) of present title compound, m.p. 117-119° C.
MS (m/e) 3S1 (M+), 363, (M+-H2O), 137, 91 (100%); high resolution 363.0936 (M+-H2<0) . IR (CKC13) 3674, 3577, 3011, 1602, 1588, 1491, 1261, 1157, 1013, 991,
852 cm 1.
1H-NMR(DMSO-d6)delta(ppm): 7.91 (t, J=7.8 Kz, 1H),
7.51 (d, J=7.8 Hz, 1H) , 7.47 (d, J=7.8 Hz, 1H) ,’ 7,157.32 (m, 5H), 6.98 (d, J=3.7 Hz, 1H), 6.85 (dd, J=9.7, 3.7 Hz, 1H), 6.71 (d, J=9.7 Hz, 1H), 5.49 (d, J=5.7 Hz, 1H) , 5.09 (s, 2H), 4.25 (t, J=5.7 Hz, 1H), 4.02 (dd, J=10.3, 3.1 Hz, 1H), 3.80 (dd, J=10.3, 6.0 Hz, 1H),
2.73 (dd, J=12.5, 6.1 Hz, 1H), 2.41 (dd, J=12.5, 8.6 Hz, 1H) and 2.03-2.10 (m, 1H).
EXAMPLE 232 trans-6-(6-Chloro-2-pyridyl)methoxy3-(3-pyridyl) methyl-4-chromanol
By the method of Example 5, 500 mg (1.95 mmol) of trans-3 - (3 -pyridyl) me thy 1-4,6-chromandiol· and 442 mg' (2.14 mmol) of 2-chloro-6-(bromomethyl)pyridine were converted to present title product, purified by flash chromatography on a silica gel column using isopropyl alcohol:ethy1 acetate :CH2Cl2, 1:2:17 as eluant, to yield 0.11 g (14%) of present title compound as a glass .
MS (m/e) 382 (M+), 256, 137, 9 (100%); high resolution 382.1039. IR (CHCip 3589, 2923, 1587,
1491, 1421, 1261, 1157, 1140, 1012, 852 cm-1.
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-1415 ^H-NMR(DMSO-άθ)delta(ppm): 8.42 (dd, J=5.0, 1.5 Hz,
IH) , 3.38 (d, J=1.2 Hz, IH), 7.85 (t, J=7 • 8 r. 2 f IH) ,
7.61 (dt, J=7.8, 1.2 Hz , IH), 7.47 (d, J= 7.8 Hz , IH) ,
7.43 (d, J=7.8 Hz, IH), 7.33 (dd, J = 7. 8, 5.0 Hz , IH) ,
6.96 (d, J=3.6 Hz, IH), 6.83 (dd, J=8. 5, 3.6 Hz , IH) ,
6.68 (d, J=8.5 Hz, IH), 5.53 (d, J=5.5 Hz , IH ) , 5.07
(s, 2H), 4.24 (t, J=5.5 Hz, IH), 4.00 (dd , J= 10 .9, 3.1
Hz, IH), 3.78 (dd, J=10 .9, 5.8 Hz, IH) , 2 .72 (d d,
J=13 .9, 6.0 Hz, IH), 2. 43 (dd, J=13.9, 8. 7 Hz t IH) and
2.01 -2.14 (m, IH).
EXAMPLE 233 trans-3-Benzy1-6-(6-methyl2-pyridyl)methoxy-4-chromanol
By the method of Example 5, 500 mg (1.96 mmol) of trans-3-benzyl-4,6-chromandiol and 401 mg of 2-(bromomethyl) -6-methyl pyridine were converted to present title product, purified by flash chromatography on silica gel using 1:1 ethyl acetate: hexane as eluant to yield 0.38 g (53%) of present title product as white crystals, m.p. 87-90° C.
MS (m/e) 361 (M+), 343 (M^-H^O), 91; high resolution
361.1692 . IR (CHCI-j) 3536 , 2923 , 1598 , 1492 , 1454 ,
1257, 1230, 1014, 681 cm-1.
XH-NMR(DMSO-dg) delta(ppm) : 7.67 (t, J = 7.8 Hz, IH) ,
7.08-7.32 (m, 7H), 6.95 (d, J=3.7 Hz, IH), 6.81 (dd, J=9.7, 3.7 Hz, IH), 6.67 (d, J=9.7 Hz, IH), 5.47 (d,
J=5.7 Hz, IH) , 5.04 (s, 2H) , 4.25 (t, J=5.7 Hz, IH) ,
4.02 (dd, J=10.3, 3.1 Hz , IH) , 3.78 (dd, J=10.3, 6.0 Hz, IH), 2.72 (dd, J=12.5, 6.1 Hz, IH), 2.40 (dd,
J=12.5, 8.6 Hz, IH) and 2.00-2.12 (m, IH).
APO00083
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-142ZXANPLE 23 4 trans — 6 — (6-Methyl-2-pyridyi) methoxy3-(3-pyridyl) methyl-4-chromanol
By the method of Example 5, 0.53 g (1.95 mmol) of 5 trans-3-(3-pyridylmethyl)-4,6-chromandioi and 400 mg (2.15 mmol) of 2-(bromomethyl)-6-methyi pyridine were converted to present title product, purified by flash chromatography on silica gel using ethyl acetate as eluant to yield purified title product, 0.14 g (20%),
m.p. 66-68° C.
MS (m/e) 362 (M+, 100%), 344 (M+-H_O), 256, 92;
high resolution 362.1615. IR (CHCi^) 3539, 2921, 1597, 1491, 1458, 1255, 1156, 1015, 850 cm1.
1H-NMR (DMSO-dg) delta (ppm) : 8.42 (dd, J = 5.0, 1.5 Hz,
15 1H) , 8.38 (d, J=1.2 Hz, 1H) , 7.70 (t, J=7. 8 Hz, 1H),
7.57 (dt, J=7.8, 1.2 Hz , 1H) , , 7.23 (dd, J= 7.8, 5.0 Hz,
•~i 1H) , 7.24 (d, J=7.8 Hz, 1H) , 7.15 (d, J=7. 8 Hz, 1H) ,
•'X 6.96 (d, J=3.6 Hz, 1H), 6.81 (dd, J=8.5, 3 .6 Hz, 1H) ,
··> 6.67 (d, J=8.5 Hz, 1H), 5.51 (d, u = 5.5 Hz, 1H -OH),
A 20 5.05 (s, 2H), 4.24 (t, J=5.5 Hz, 1H), 4.00 (cd, J=10.9,
£9* 3 . 1 Hz, 1H), 3.78 (dd, J=10. ( 5 , 5.8 Hz, 1H) , 2.72 (dd,
J=13.9, 6.0 Hz, 1H), 2.43 (dd, J=13.9, 8.7 Hz, 1H) and 2.02-2.14 (m, 1H).
EXAMPLE 235 trans-6-(2-Pyridy1)methoxy-3(3-pyridyl)methyl-4-chromanol
By the method of Example 5, 500 mg (1.95 mmol) of trans-3-(3-pyrldyl methyl)-4,6-chrcmandiol and 266 mg (2.09 mmol) cf 2-picolyl chloride were converted to present title product, purified by flash chromatography on 100 g silica gel using 1:19 CH3OH:ether as eluant to yield 136 mg (20%) of present title compound as an oil.
BAD ORIGINAL ft
3C
-143MS (m/e) 3 4 8 (M ) , 256 , 9 2 ( 10 0%); high resolution 348.1429. IR (CKCIJ 3592, 2957, 1595, 1491, 1262, 1206, 1015 cm-1.
1H-NMR(DMSO-d6)delta(ppm): 8.54 (dt, J=4.8, 1.8 Hz,
1H), 8.40 (dd, J=5.0, 1.5 Hz, 1H), 8.38 (d, J=1.2 Hz, 1H) , 7.81 (dt, J=1.8, 7.8 Hz, 1H) , 7.60 (dt, J=7.8, 1.2 Hz, 1H), 7.49 (d, J=7.8 Hz, 1H) , 7.28-7.36 (m, 2H) ,
6.98 (d, J=3.6 Hz, 1H)., 6.84 (dd, J=8.5, 3.6 Hz, 1H) , 6.69 (d, J=8.5 Hz, 1H), 5.53 (d, J=6 Hz, 1H, -OH), 5.10 (s, 2H), 4.26 (t, J=6 Hz, 1H), 4.03 (dd, J=10.9, 3.1 Hz, 1H), 3.80 (dd, J=10.9, 5.8 Hz, 1H), 2.73 (dd, J=13.9, 6.0 Hz, 1H), 2.46 (dd, J=13.9, 8.7 Hz, 1H) and 2.04-2.16 (m, 1H).
EXAMPLE 236 cis-6-(3-Bromo-6-methyl-2-pyridyl)methoxy-3-(3-pvridyl)methyl-4-chromanol
By the method of Example 5, 203 mg (0.79 mmol) of the title product of Example 4A and 218 mg (0.82 mmol) of 3-bromo-2-(bromomethyl)-6-methylpyridine were converted to present title product, purified by flash chromatography on silica gel using ethyl acetate as eluant to yield 93 mg (38%) of purified title compound as a glass.
MS (m/e) 440 (M+), 442 (M+-H2O), 256 (100%), 92; high resolution 440.0682. IR (CHCl-j) 3538, 2949 , 1576, 1491, 1443. 1275, 1237, 1192, 1151, 1020 cm-1.
1H-NMR(DMSO-dg)delta(ppm): 8.49 (d, J=1.2 Hz, IK),
8.44 (dd, J=5.0, 1.5 Hz, 1H) , 7.97 (d, J=8.2 Hz, 1H) , 7.74 (dt, J=7.8, 1.2 Hz, 1H), 7.35 (dd, J=7.8, 5.0 Hz, 1H), 7.22 (d, J=8.2 Hz, 1H), 6.92 (d, J=3.5 Hz, 1H), 6.86 (dd, J=9.0, 3.5 Hz, 1H), 6.69 (d, J=9.0 Hz, 1H), 5.47 (d, J=5.5 Hz, 1H, OH), 4.30 (t, J=5.5 Hz, 1H) ,
3.93 (d, J=6.9 Hz, 2H), 2.82 (dd, J=12.6, 7.7 Hz, 1H), 2.59 (dd, J=12.6, 7.3 Hz, 1H), 2.44 (s, 3H), 2.18-2.32 (m, 1H) .
AP 0 0 0 0 8 3
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EXAMPLE 237 cs.s-6- (5-3rcmo-6-methyl-2-pyridyl) methoxy-3 -(3-pyridyl)methy1-4-chromano 1
By the method of Example 5, 240 mg (0.93 mmol) of 5 the title product of Example 4A and 270 mg (1.02 mmol) of 3-bromc-6-(bromomethyl)-2-methylpyridine were converted to present title product, purified by recrystallization from ether to yield 65 mg (16%) as a white solid, m.p. 134-137° C.
MS (m/e) 440 (M+), 44 2 (M+-H 2O), 256 (100%) , 92;
high resolution 440.0714.
1H-X1 MR(CDC1-)delta(ppm): 3.59 (d , J = 1 . 2 Hz, IK) , 8.51
(dd, J=5.0, 1.5 Hz, 1H), 7 .80 (d, J=7.8 Hz, 1H), 7.69
(dt, J=7.3, 1.2 Hz , 1H) , 7 .33 · (dd , J=7.8, 5.0 Hz , 1H) ,
7.19 (d, J=7.8 Hz, 1H), 6. 75-6.90 (m, 3H), 5.01 (s,
2H) , 4.45 (d, J=5.5 Hz, 1H ), 4.07 (d, J=8.4 Hz, 2H) ,
2.95 (dd, J=12.6, 7.7 Hz, 1H), 2. 70 (dd, J=12.6, 7.3
Hz , 1H), 2.63 (s, 3H) and 2.22-2. 40 (m, 1H) .
·» a
EXAMPLE 238 cis-(6-Methyl-2-pyridyl)methoxy3-(3-pyridyl)methv1-4-chromanol
By the method cf Example 5, 500 mg (1.95 mmol) of the title product of Example 4A and 544 mg (2.93 mmol) of 2-(bromomethyl)-6-methvlpyridine were converted to present title product, purified by flash chromatography on silica gel using 1:19 CH3OH:ether as eluant to yield 204.2 mg (29%) of the present compound.
IR (CHCi3) 3591, 2952, 1597, 1491, 1459, 1425, 1277, 1242, 1152, 1073, 1023 cm-1.
BAD ORIGINAL ft
-145AH-NMR(DMSC-dg)delta(ppm): 8.46 (d, J=1.2 Hz, IK),
8.39 (dd, J=5.0, 1.5 Hz, 1H), 7.62-7.72 (m, 2H), 7.30 (dd, J=7.8, 5.0 Hz, 1H) , 7.23 (d, J=7.8 Hz, 1H) , 7.14 (d, J=7.8 Hz, 1H), 6.86 (d, J=3.6 Hz, IK), 6.80 (dd, J=9.0, 3.5 Hz, 1H), 6.66 (d, J=9.0 Hz, 1H), 5.41 (d,
J=5.5 Hz, 1H), OH), 5.02 (s, 2H), 4.28 (t, J=5.5 Hz,
1H), 3.91 (d, J=6.9 Hz, 2H), 2.77 (dd, J=12.6, 7.7 Hz, 1H), 2.53 (dd, J=12.6, 7.3 Hz, 1H), 2.46 (s, 3H) and 2.10-2.27 (m, 1H).
EXAMPLE 239
6-(6-Fluorc-2-quinolyl) methoxy-3(3-ovridvloxv)-4-chromanone
By the method of Example 229, Method B, the title product of Example 75 (500 mg, 1.2 mmol) was converted to present title product, purified by flash chromatography on silica gel using 22:1 CH2C12:CH3OH as eluant, 202 mg; m.p. 188° C.; MS calculated: 416.1176; found: 416.0798.
EXAMPLE 240 (-)-cis-(6-Fluoro-2-quinolyi)methoxy-3(3-pyridyloxy)-4-chromanyl Dimethylglycinate Ester
By the method of Example 117, the title product of
Example 115 (608 mg, 1.4 mmol) was converted to present title product, 578 mg; tic (5:1 CH2C12: isopropanol) Rf
0.3; MS 503 (M+). By the method of Example 118, except to use 4 molar equivalents of HCl, this product was converted to its trihydrochloride salt, recrystallized from isopropanol and ether, 584 mg; m.p. 160-165° C.
(degassing), 180° C. (dec.); IR 1775 cm
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-14 6·.£ 'i
Ο
EXAMPLE 241 (-)- and (-)-cis-3-(4-Methoxyphenoxy)-6( 2-pyridyl)methcxy-4-chromanol
3y the methods of Examples 16-18, the title product of Example 110 (14 g) was resolved into present title products. Initial separation of the intermediate . diastereomeric esters was achieved using gradient elution with 5:1, 4:1, 3:1 and finally 1:1 toluenezethyl acetate to yield 11.87 g of the pure, less polar, (-)-cis isomer and 19.51 g of more polar, (+)-cis isomer, contaminated with some lp isomer. The latter was recnromatographed using 3:1, then 2:1 toluene: ethyl acetate to yield 15.32 g of the pure, more polar (+)-cis isomer. Hydrolysis per Example 18 gave:
title (-)-cis isomer, 7.17 g; m.p. 117-118.5° C.;
exact mass calculated: 379.1454; found: 379.1437. Analysis calculated for ^22^1^5: «
C, 69.64; H, 5.58; N, 3.69%.
Found: C, 69.53; H, 5.59; N, 3.77%.
title (+)-cis isomer, 8.21 g; m.p. 115.5-117.5° C.;
exact mass calculated: 379.1420; found: 379.1282.
Analysis calculated as for (-)-isomer. Found: C,
69.46; H, 5.51; N, 3.74%.
EXAMPLE 242 (-)-cis-3-(4-Methoxyphenoxy)-6-(2-pyridyl)methoxy-4-chromanyl Dimethylglycinate Ester
By the method of Example 117, the (-)-cis title product of the preceding Example (1.0 g) was converted to present title product, purified by chromatography on silica gel gradiently eluting with 7:1, 5:1 and 3:1 toluene: isopropanol to yield purified title product,
1.20 g; tic Rf 0.1 (7:1 toluene: ethyl acetate); MS 464 (M+). The latter was converted to its dihydrochioride
BAD ORIGINAL ft
-147salt according to Example 118 and recrystallized from isopropar.ol to yield 1.12 g of dihydrochloride; m.p. 200-203° C.; IR 1757 cm-1.
Analysis calculated for Ο^Η^ϊ^Οθ · 2HC1:
C, 57.61; H, 5.26; N, 5.17%.
Found: C, 57.60; H, 5.62; N, 5.16%.
By the same method, the title product of Example 111 (0.41 g, 0.916 mol) was converted to (+)-cis-3-(4methoxyphenoxy)-6-(6-fluoro-2-quinolyl)methoxy-4chromanyl dimethylglycinate ester dihydrochloride,
0.44 g; m.p. 190-195° C.; 1H-NMR(250 MHz, DMSO-dr) includes delta 3.71 (s, 3H), 2.81 (s, 3H), 2.85 (s, 3H) and 5.34 (s, 2H).
Prepared in like manner were (+)-cis-6-(6-fluoro2-quinolyl)methoxy-3-(3-pyridyloxy)-4-chromanyl dimethylglycinate trihydrochloride [m.p. 215-217° C.; ^H-NMR (same conditions) includes delta 2.84 (s, 6H), 5.39 (s, 2H) ] ; and (+)-cis-6-(2-pyridyl)methoxy-3-(3-pyridyloxy)4-chromanyl dimethylglcinate ester trihydrochloride [m.p. 190-200° C.; ^H-NMR (same conditions) includes 2.85 (s, 6H) and 5.33 (s, 2H) ] .
EXAMPLE 243 (+)- and (-)-cis-3-(3-Pyridyloxy)-6(2-pyridyl)methoxy-4-chromanol
By the method of Examples 114-116, the title product of Example 78 (1.52 g) was resolved into title products. The intermediate carbamate diastereoisomers were separated by silica gel chromatography using CHCl^: isopropanol as eluant followed by hplc on a Zorbax Sil packed column using 19:1 CHCl^ as eluant to yield 986 mg of lp, (-)-cis diastereomer and 875 mg of ap η η ο 0 8 3
BAD ORIGINAL ft purified rr.p M-cis isomer. Hydrolysis of these diastereomers according to Example 115 gave:
title (-)-cis isomer, purified by chromatography using 7:1 CH^^ : isopropar.ol as eluant and recrystalli5 zation from toluene, 338 mg; m.p. 146.5-148.5; exact mass calculated: 350.1267; found: 350.1315.
Analysis calculated for C„nH :
18 2 4
C/ 68.56; H, 5.18; N, 8.00%.
Found: C, 68.27; H, 5.09; N, 7.97%.
[alpha]D = -39° (c = 0.1 CHCl-j) .
title (+)-cis isomer, likewise chromatographed and recrystallized, 312 mg; m.p. 150.5-151.5; exact mass calculated as above; found; 350.1267.
Analysis calculated as above. Found: C, 67.91; H,
5.07; N, 7.98%.
[alpha]D = +39° (c = 0.1 CHC13).
EXAMPLE 24 4 (-)-cis-3-(4-Methoxyphenoxy)-6-ζ (2-quinolvl) methoxy-4-chromanol
By the method of Examples 16-18, title product of
Example 21 (9.10 g) was resolved via diastereomeric
5* E(-)-O-acetylmandellate esters. Chromatography of these esters on silica gel using 49:1 C^C^: isoPr°Pan°f as eluant gave the pure, less polar (-)-cis ester, recrystallized from 1:1 toluene: hexane, 1.87 g; and 8.83 g of mixed (-)-cis and (+)-cis (less polar and more polar, respectively) suitable for recycling and further separation.
The pure (-)-cis ester was hydrolyzed according to
Example 17 to yield present title product recrystallized from toluene, 1.13 g; m.p. 154-156° C. [alpha]D = -52.8° (c = 0.1 CHC13) .
BAD ORIGINAL
-149ΞΧAMPLE 245 (+)- and (-)-cis-3-(3-Pyridyloxy)-6(2-quinolyl)methoxv-4-chromanol
By the method of Examples 16-18, title product of 5 Example 78 (1.28 g) was converted to present title products. The intermediate diastereomeric esters were separated by chromatography using 53:43 CHCl^ihexane containing 0.5% triethylamine. Following hydrolysis, each product was recrystallized from toluene to yield:
(+)-cis isomer, 292 mg; m.p. 156.5-158.5° C.;
exact mass calculated: 400.1423; found: 400.1395.
Analysis calculated for CO .H^nN„O . · 0.25?' 0:
z4 zu Z 4 2
C, 71.18; H, 5.10; N, 6.92%.
Found: C, 71.39; H, 4.92; N, 6.77%.
[alpha]D = +40.6°.
(-)-cis isomer, 171 mg; m.p. 152-153.5° C.; exact mass calculated as above; found: 400.1418.
Analysis calculated for C24H20N2°4’θ’^^H20:
C, 69.63; H, 5.23; N, 6.77%.
Found: C, 69.83; H, 4.84; N, 6.58%.
EXAMPLE 246 (+)-cis-3-(3-Pyridyloxy)-6-(2-quinolyl)methoxy4-chromanyl DimethyIglycinate Ester Trihydrochloride
By the methods of Example 240, title product of
Example 76 (250 mg, 0.87 mmol) was converted to present title product, 320 mg; m.p. 165° C. (degassing); exact mass calculated: 485.1953; found: 485.1929.
By the same methods, the title products of the preceding Example were each converted to the correspond30 ing optically active forms:
£ 8 0 0 0 0 dV
BAD ORIGINAL
-150(+)-cis isomer, IS! mg from 1S4 mg; exact mess calculated: 4 3 5.1954 ; found: 485.1975.
Analysis calculated for CnnH-„0-N^·3HC1·2H_0:
Z 0 Z / 0 J Z
C, 53.29; H, 5.43; N, 6.66%.
Found; C, 53.42; H, 5.29; N, 6.61%.
(-)-cis isomer, 228 mg from 235 mg.
Analysis calculated for C28K27N3°5*3HC1*2 *5H2°:
C, 52.54; H, 5.51; N, 6.57%.
Found: C, 52.56; H, 5.35; N, 6.63%.
10 EXAMPLE 247
'v.
ο ο
JO >
( + )-cis-3-(4-Methoxyphenoxy)-6-(4-methoxy2-pyridyl)methoxy-4-chromanol
By the method of Example 13, the title product of Example 20 (494 mg, 3 mmol) and freshly prepared
4-methoxy-2-picolyl chloride (903 mg, 3 mmol) were converted to present title product, purified by chromatography on silica gel using 51:25:4 toluene:ethyl acetate: isopropanol as eluant and recrystallization from toluene, 564 mg; m.p. 80-82° C.; tic Rf 0.3 (19:1
CH2C12: isopropanol); exact mass calculated: 409.1531; found: 409.1530.
Analysis calculated for C23H23N°6:
C, 67.47; H, 5.66; N, 3.42%.
Found: C, 67.13; H, 5.77; N, 3.39%.
25 EXAMPLE 248 (+)-cis-3-(3-Methoxyphenoxy)-6-(4-methoxy2-pyridvl·)methoxy-4-chromano 1
According to the methods of the preceding Example, title product of Example 25 (267 mg, 1.7 mmol) was converted to present title product, 112 mg; m.p.
165-166° C.; exact mass calculated: 409.1531; found: 409.1540.
€>/·!. U
OFUGINAL ft
-151EXAMPLE 249 (t)~cis-6- (4-Me thoxy-2-pyridyl) methoxy-3-(3-pyridyimethyl)-4-chromanol and its Dihydrochloride
By the method of the preceding Example, title product of Example 4A (612 mg) was converted to crude title product in free base form. The latter was taken up in 25 ml of methanol and 2N HC1 (2.7 ml, was added. After stirring for 15 minutes, the mixture was stripped in vacuo. The residue was stirred with 20 ml of toluene and restripped three times and the residue triturated with ethyl acetate to yield present title dihydrochloride product, 1.07 g; m.p. 113° C., (degasses) 135° C., (dec .) .
This preparation was repeated on 266 mg of the same starting material with purified free base of title product obtained by silica gel chromatography using 3:3:2 toluene: ethyl acetate: isopropanol as eluant and recrystallization from CH2C12» 119 mg; m.p. 66.5-68.5; exact mass calculated: 380.1372; found: 380.1379.
Analysis calculated for ί-21^20^2θ5 :
C, 66.30; H, 5.30; N, 7.36%.
Found: C, 66.55; H, 5.29; N, 7.24%.
EXAMPLE 250 (+)-cis-3-(3-(Methoxycarbonyl)benzyl)25 6-(2-pyridvl)methoxy-4-chromanol
By the method of Example 13, the mixed title product of Example 46 (1.0 g, 3.18 mmol) and 2-picolyl chloride (444 mg, 3.48 mmol) were converted to a mixture of title product and the corresponding trans30 isomer. Present title product was separated by two-fold silica gel chromatography, first with 33:1 CH2Cl2:isopropanol as eluant followed by 3:2 toluene:ethyl acetate as eluant to yield present, purified title product,
422 mg.
AP 0 0 0 0 8 3
BAD ORIGINAL £
EXAMPLE 251 ( + ) -cis-3-(3-Carboxybenzvl) 6-72-pyridyl) methoxy-4-chromanol
Title product of the preceding Example (422 mg) was combined with 11 ml of methanol and 5 ml IN NaOH and then heated at reflux for 15 minutes, cooled, stripped in vacuo and the residue combined with 20 ml of toluene and restripped. The dried residue was chromatographed on silica gel using 6:1 CH2C12:CH3OH as eluant,'and recrystallized from isopropyl ether/CH2Cl0/ hexane to yield purified title product, 254 mg; m.p. 196.5-197 .-5° 0., exact mass calculated: 391.1424; found: 391.1425.
Analysis calculated for ^23Η21Νθ5*θ^H20:
C, 68.98; H, 5.29; N, 3.49%.
Found: C, 69.10; H, 5.36; N, 3.58%.
EXAMPLE 252 ' -- x
6-(2-Pyridyl)methoxy-3-(3pyridyloxy)-4-chromanone
..., 20 By the method of Example 229, Method B, the title product of Example 78 (150 mg, 0.43 mmol) was converted > to present title product, purified by silica gel chromatography using 19:1 CH2C12: isopropanol as eluant and recrystallization from toluene, 63 mg; m.p. 155-156.5° C., exact mass calculated: 348.1114; found: 348.1035. Analysis calculated for C^H^gN^^· 1.25K2O:
C, 64.75; H, 5.03; N, -7.55%.
Found: C, 64.94; H, 4.77; N, 7.29%.
BAD ORIGINAL Ά
-153EXAMPLE 253
3- (3-Pvridyloxy) -6- (2-quinolyl·) methoxy-4-chromanone
By the method of Example 229, Method B, the title product of Example 76 (527 mg) and/or the corresponding trans-isomer was converted to present title product, purified by silica gel chromatography using 1:1 toluene:ethyl acetate containing 1% glacial acetic acid as eluant, 216 mg; m.p. 174-175” C., exact mass calculated: 398.1266; found: 398.1232.
Analysis calculated for C_,Η.oN_0 , :
18 2 4
C, 72.34; H, 4.55; N, 7.03%.
Found: C, 72.10; H, 4.49; N, 6.97%.
EXAMPLE 254
6-Benzyloxy-3- (6-methyl-3-pyridyloxy) -4-chromanone 15 2-Methyl-5-hydroxypyridine (8.18 g, 0.025 mol) and
6-benzyloxy-3-bromo-4-chromanone (25.0 g, 0.075 mol) were converted to present chromatographed title product by the method of Example 72, 1.34 g; tic Rf 0.25 (1:4 ethyl acetate:CH2Cl2); IR (CHCl-j) 1702, 1484 cm-1.
By the same methods, 2-methyl-3-hydroxypyridine (7.30 g, 0.067 mol) was converted to 1.17 g of 6-benzyloxy-3- (2-methyl-3-pyridyloxy) -4-chromanone (^H-NMR includes delta 2.43 (s, 3H), 4.6 (m, 2H) and 4.93 (dd, 1H); MS includes 361 (M+) and base peak at
91; IR (CHCl.^) 1697, 1486 cm-1].
EXAMPLE 255 ( + ) -cis-6-Benzyloxy-3-(6-methyl-3pyridyloxy)-4-chromanol
By the method of Example 4, title product of the preceding Example (1.33 g, 0.0037 mol) was converted to present title product, 1.40 g, evidently contaminated with 10-15% of trans-isomer by ^Ή-ΝΜΚ; tic Rf 0.4 (19:1 CH2C12:CH3OH); IR (CHCip 3562 cm-1.
£ 8 0 0 0 0 dV
BAD ORIGINAL ft the same method, the isomeric product of the preceding Example (1.16 c, 3.21 mmol) was converted to (+)-cis-6-benzyloxy-3-(2-methy1-3-pyridyloxy)-4-chromanol, 1.12 g; m.p. 133-134° C.; tic Rf 0.28 (1:19
CH3OH: CH2C12; Rf 0.58 (1:9 CH3OH:CH’2C12) .
EXAMPLE 256 (+)-cis-3-(6-Methyl-3-pyridyloxy)-4,6-chromandiol
By the method of Example 10, using 2:1 CH3OH:THF as solvent, title product of the preceding Example was converted to present title product, purified by silica gel chromatography initially using 19:1 and then 9:1 CH2C12:CH3OH as eluant, 0.85 g; tic Rf 0.14 (9:1 CH2C12:CH3OH).
By the same method, the isomeric product of the preceding Example (1.02 g) was converted to (+)-cis-3(2-methyl-3-pyridyloxy)-4,6-chromandiol, 395 mg; tic Rf 0.24 (1:9 CH3OH:CH2C12); m.p. 240-241° C.
EXAMPLE 257 (+)-cis-6-(6-Fluoro-2-quinolyl)methoxy-320 (6-methy1-3-pyridyloxy)-4-chromanol
By the method of Example 13, title product of the preceding Example (250 mg, 0.92 nmol) and (6-fluoro-2quinolyl)methyl chloride (179 mg, 0.92 mmol) were converted to present title product, purified by silica gel chromatography using gradient elution with 1:50, 1:19 and 1:10 CII-jOH :CH2C12 , 262 mg; tic Rf 0.28 (9:1 CH3OH:CH2C12), m.p. 164-165° C.; IR (KBr) 1501, 1483 cm'
BAD ORIGINAL &
-1553y the same method, the isomeric product of the preceding Example (270 mg, 0.99 mmol) was Converted to ( + )-cis-6-(6 — fluoro-2-quinolyl)methoxv-3-(2-methyl-3pyridyloxy)-4-chromanol, 325 mg; m.p. 158-159° C.; tic
Rf 0.37 (1:9 CH^OHzCL^Cl^); exact mass calculated; 432.1486; found; 432.1469; IR (KBr) 1491, 1457 cm-1.
EXAMPLE 258 ( + ) -cis-3-(6-Methyl-3-pyridyloxy)-6(2-guinolyl)methoxy-4-chromanol
By the method of the preceding Example, except to use 1:50 and then 1:19 CH3OH:CH2C12 as eluant, title product of Example 256 (420 mg, 1.54 mmol) and (2-quinolyl)methyl chloride (274 mg, 2.54 mmol) were converted to present title product, 520 mg; m.p.
134-136° C.; IR (KBr) 1618, 1571, 1494 cm1; tic Rf 0.4 (9:1 CH2C12:CH3OH).
By the same method, the isomeric product of Example 257 (300 mg, 1.10 mmol) was converted to (+)-cis-3-(2-methy1-3-pyridyloxy)-6-(2-quinolyl)methoxy20 4-chrcmanol, 390 mg; m.p. 159-160° C.; tic Rf 0.35 (1:9 CF3OH:CH2C12); 1H-NMR includes delta 5.33 (s, 2H), 2.39 (s, 3H); IR (CHC13) 3564, 1491 cm 1; exact mass calculated: 414.1581; found: 414.1580.
EXAMPLE 259 (+)- and (-)-cis-3-(6-Methy1-3-pyridyloxy)-6(2-quinolyl) methoxy-4-chromanol via
Esters with N-(t-Butoxycarbonyl)-L-tryptophane
Substituting a molar equivalent of N-(t-butoxycarbony1-L-tryptophane for the O-acetyl mandelic acid in the method of Example 16, title product of the preceding Example (4.73 g, 11.4 mmol) was converted to present diastereomeric title products, separated by silica gel chromatography using 21:7:1 CHC13: hexane : isopropanol as eluant to yield 2.1 g of the less polar
ΔΡ 0 0 0 0 8 3
BAD ORIGINAL sa (+)-cis isomer and 1.51 g of the more polar (-)-cis isomer. These esters were hydrolyzed by stirring for 1 hour in aqueous methanol (20 ml methanol and 8 ml of IN NaOH for each gram of ester). The reaction mixtures were diluted with water (20 ml/g), adjusted to pH 7 with 2N HC1 and resolved title products recovered by filtration and purified by recrystallization from toluene:
(+)-cis-title product, 715 mg; m.p. 128.5-130° C.; 10 exact mass calculated: 414.1580; found: 414.1572.
Analysis calculated for ’H2°:
C, 69.34; K, 5.59; N, 6.48%.
Found: C, 69.34; H, 5.20; N, 6.30%.
[alpha)D = +44.7°.
(-)-cis-title product, 720 mg; exact mass calculated as above; found: 414.1564.
[alpha)D = -44.2°.
EXAMPLE 260 ( + )- and (-)-cis-3-(6-Methyl-3-pyridyloxy) 20 6-(2-quinolyl)methoxy-4-chromany1
Dimethylglycinate Esters Trihydrochlorides
By the methods of Example 240, the title products of the preceding Example (250 mg of each) were converted to present title products:
(+)-cis-title product, 317 mg; m.p. 155° C.
(degassing) 180° C. (dec).
(-)-cis-title product, 220 mg; m.p. identical, as expected.
BAL· ORIGINAL Ά
-15710
EXAMPLE 251
6-Hethoxy-3- (5-pyrimidy1) me‘chylene-4-chromanone
By the method of Example 1, 6-methoxy-4-chromanone (6.76 g, 0.033 mol) and pyrimidine-4-carbaldehyde (4.14 g, 0.038 mol) were converted to present title product, purified by flash chromatography on silica gel using 45:1 CH2C12: isopropanol as eluant, 2.03 g; MS 268 (M+) .
EXAMPLE 262
6-Methoxy-3-(5-pvrimidylmethyl)-4-chromanone
By the method of Example 2, title product of the preceding Example (2.03 g) was converted to present title product without trituration, 2.96 g; MS 270 (M+).
EXAMPLE 263
6-Hydroxy-3-(5-pvrimidylmethyl)-4-chromanone
By the method of Example 3, title product of the preceding Example (2.05 g, 0.0076 mol) was converted to present title product, purified by the chromatographic method of Example 261, 269 mg; MS consistent with product.
EXAMPLE 264 (+)-cis-3-(5-PyrimidyImethyl)-4,5-chromandio1
AP 0 0 0 0 8 3
By the method of Example 4, the title product of the preceding Example (264 mg, 1 mmol) was converted to present title product, purified by chromatography on silica gel using 19:1 CK2C12:CH3OH as eluant, 125 mg; tic Rf 0.18 (19:1 Ch’2Cl2 :Ch'3OH) .
EXAMPLE 265 (+)-cis-6-(6-?luoro-2-cuinoiyl)methoxy-33 0 _( 5-pyrimidylmethyl) -4-chromanol_
By the method of Example 75, title product of the preceding Example (125 mg, 0.48 mmol) was converted to present title product using first 14:1 and then 9:1 CH2C12: isopropanol as eluant, 37 mg; m.p. 133-191° C.;
exact mass calculated: 417.1489; found: 417.1508.
BAD ORIGINAL ft
-158EXAMPLE 266
5-Eenzyioxy-3-(3-(methoxycarbonvl) phenoxy)-4-chromanone
By the method of Example 72, 6-benzyloxy-3-bromo5 4-chromanone (49.9 g, 0.15 mol) and methyl 3-hydroxybenzoate (22.8 g, 0.15 mol) were converted to present title product purified by chromatography on silica gel using CH2C12 as eluant, 2.19 g; tic Rf 0.22 (CH2C12).
EXAMPLE 267 (+)-cis-6-Benzyloxy-3-(3-(methoxycarbonyl) phenoxy-4-chromanone Title product of the preceding Example (2.18 g,
5.4 mmol) was dissolved in 120 ml of THF. CeCl^ 7H2O) (1.20 g, 0.32 mmol) was added and the mixture cooled to
-40° C. and stirred under N2· NaBH^ (0.204 g, 0.54 mmol) was added and stirring continued for 25 minutes. To achieve complete conversion to product, additional CeCl^ 7H2O (1.0 g) and NaBH^ (0.102 g) were added ana stirring continued for 15 minutes. The reaction was quenched by adding 2 ml of acetone and warming the reaction mixture to room temperature. It was then stripped of solvent and the residue distributed between 150 ml H_0 and 100 ml ethyl acetate. The aqueous layer was separated and extracted 1 x 100 ml fresh ethyl acetate. The organic layers were combined, washed with brine, dried (Na-^SO^) , stripped to 2.41 g of oil and chromatographed on silica gel using 1:19 ethyl acetate:CH2C12 as eluant, 1.34 g; tic Rf 0.52 (1:9 CH..OH : CH 9C1 _ ) .
BAD ORIGINAL &
-159EXAMPLE 263 (+)-cis-3-(3-(Methoxycarbonyl)phenoxy-4,6-chromandiol
By the method of Example 12, the title product of 5 the preceding Example (1.34 g) was converted to present title product, purified by silica gel chromatography using 1:4 ethyl acetatezCl^Clj alone and then with 1% CH3OH as eluants, 935 mg; tic Rf 0.30 (1:19 CH3OH:CH2C12); MS 316 (M+), 138 (base peak).
EXAMPLE 269 (+)-cis-6-(Substituted)methoxy-3-(3(methoxycarbonyl) phenoxy) -4-chrorranol
By the method of Example 15, title product of the preceding Example was converted to the following present title products:
(a) 6-(5-fluoro-2-benzthiazolyl)methoxy derivative, 726 mg from 547 mg (1.73 mmol), using gradient elution with 1:99, 1:49 and 1:24 CH3OH:CH2C12; tic Rf 0.48 (1:19 CH3OH:CH2C12)? MS 481 (M+) , 166 (base peak); IR 20 (CHC13) 1722, 1489 cm-1.
(b) 6-(2-quinolyl)methoxy derivative, 411 mg from 322 mg (1.02 mmol), using 1:99 and then 1:49 CH3OK:CH2C1? as eluant; tic Rf 0.47 (1:19 CH3CK:CH2C12); MS 457 (M+), 142 (base peak); IR (KBr) 1725, 1499 cm-1.
(c) 6-(6-fluoro-2-quinoly1) methoxy derivative
454 mg from 320 mg (1.01 mmol), eluant as (b); tic Rf 0.63 (1:19 CK-jOH:CH2Cl2) ; IR (KBr) 3415, 1722 cm1.
(d) 6-(2-pyridyl)methoxy derivative, 363 mg from
359 mg (1.13 mmol), eluant as (a); tic Rf 0.29 (1:19
CH-,ΟΗ : C .i _C1 „) ; MS 407 (M+) , 93 (base peak); IR (CHC1.)
3565, 1721, 1490 cm .
AD ft ft ft ft ft bad original $
-160VO J s
EXAMPLE 270 ( + )-cis-6-(Substituted) methoxy-3(3-carboxyphenoxy) - 4-chromanol
3v the method of Example 251, the products of the 5 preceding Example were hydrolyzed to present title products as follows:
(a) 6-(5-fluoro-2-benzthiazolyl)methoxy derivative, 383 mg from 620 mg, recrystallized from 1:19 CH3OH:CH2C12; m.p. 211-212° C.; tic Rf 0.27 (1:9 CH3OH:CH2C12); exact mass calculated: 467.0839; found: 467.0658.
(b) 6-(2-quinolyl)methoxy derivative 336 mg from 400 mg, not recrvstallized; m.p. 144-145° C.; tic Rf 0.30 (1:9 CH3OH:CH2C12); MS 443 (M+), 142 (base peak); exact mass calculated: 443.1369; found: 443.1468.
(c) 6-(6-fluoro-2-quinolyl)methoxy derivative,
306 mg from 445 mg, not recrystallized; m.p. 128-130° C.; tic Rf 0.27 (1:9 CH3OH:CH2C12); MS 461 (M+), 160 (base peak); IR (KBr) 1699, 1498 cm exact mass calculated: 461.1275; found: 461.1253.
(d) 6-(2-pyridyl)methoxy derivative, 79 mg from
344 mg, chromatographed with'1:9 CH3OH:CH2C12 as eluant and recrystallized from 1:19 CH3OH:CH2C19; m.p.
174-176° C.; tic Rf 0,20 (1:9 CH^OH:CH2C12); MS 393 (M+; base peak), 137; IR (KBr) 3325, 1703, 1498 cm-1.
BAD ORIGINAL &
-161EXAMPLE 271 (+)- and (-)-trans-3-(3-PyridyImethyl)¢-(2-cuinolyl)methoxy-4-chromanol
By the methods of Examples 6, 7 and 8, trans-title product of Example 5 (3.30 g, 8.28 mmol) was resolved via its diastereomeric R- (-)-O-acetylmandelate esters:
diastereomer A, 1.2 g; MS 574 (M+), 142 (base peak); IR (KBr) 1743, 1673, 1618, 1600, 1575 cm1.
diastereomer B, 0.9 g; m.p. 108-110° C.; MS 574 (M+) , 142 (base peak); IR (KBr) 1745, 1671, 1617, 1599 , 1574 cm-1.
Analysis calculated for C-,cH,nNo0, · 0.25H„O:
J J JU z. D 2
C, 72.59; H, 5.31; N, 4.84%.
Found: C, 72.55; H, 5.15; N, 4.77%.
Following hydrolysis, title products were obtained as follows:
(-)-trans-isomer (from A), 0.88 g; m.p. 150151° C.; [alpha] = -30.8° (CH3OH, c=0.006); IR (KBr) 1638, 1621, 1601, 1575 cm-1.
Analysis calculated for 0.251^0:
C, 74.52; H, 5.63; N, 6.95%.
Found: C, 74.68; Η, 5 ·51; X, 7.10%.
( + ) -trans-isomer (from B) , 0.84 g; m.p. 151AP 0 0 0 0 8 3
152.5° C.; [alpha] identical with (-)-isomer.
= +30.6° (CH3OH, c=0.005); IR
BAD ORIGINAL £
6-Methoxv-3- ( 4-:
dvl) ist'av
By the method of Example 1, 6-methoxy-4-chromanone (26.7 g, 0.15 mol) and pyridine-4-carbaldehyde were converted to present title product, 13.5 g; m.p. 170171.5° C.; IR (KBr) 1675, 1616, 1598, 1552 cm-1. Analysis calculated for C^gH^NO^:
C, 71.90; H, 4.90; N, 5.24%.
Found: C, 71.76; H, 4.90; N, 5.29%.
EXAMPLE 273
6-Methoxy-3- (4-pvridylmethyl)-4-chromanone By the method of Example 2, title product of the preceding Example (3.50 g,) was converted to present title product, recrystallized from ethyl acetate/hexane, 3.2 g; m.p. 91-92.5° C.; MS 269 (M+), 150 (base peak);
IR (KBr) 1676, 1618, 1604, 1588, 1561 cm-1.
Analysis calculated for C^gH-^NO^ :
C , 71.36; H, 5.61; N , 5.2 0 % .
Found: C, 71.35; K, 5.58; N, 5.03%.
EXAMPLE 27 4
6-Hvd:
• 3- {4-cvridvlmethvl)-4-chromanone
By the method of Example 3, the title product of the preceding Example (7.5 g) was converted to present title product, recrystallized from ethyl acetate,
5.2 g; m.p. 188-189.5° C.; MS 255 (M+), 93 (base peak); IR (KBr) 1633, 1633, 1611, 1587, 1560 cm 1.
BAD ORIGINAL ft
-163EXAMPLE 275 cis- end trans-3-(4-Pyridylmethyl)-4,6-chromandiol
By the method of Example 5, title product of the 5 preceding Example (5.0 g, 19.6 mmol) was converted to a mixture of title products, 4.8 g; MS 257 (M+) ; IR (KBr) 1610, 1561 cm1; XH-NMR (300 MHz, CDCl-j) includes delta 4.18 and 4.26 (bs, ratio of 2.7:2.1, CHOH).
EXAMPLE 276 (+)-cis- and (+)-trans-3-(4-Pyridylmethyl)_6-(2-quinolyl)methoxy-4-chromano!_
By the method of Example 5, except to use KOC(CK3)3 as base, the mixed title product of the preceding Example (4.0 g, 0.016 mol) was converted to present title products, separated by chromatography on silica gel using 8:1:1 CH2Cl2:ethyl acetate:diisopropyl ether as eluant to yield title products as follows:
(+)-cis-isomer, recrystallized from chloroform-diisopropyl ether, 1.2 g; m.p. 115-117° C.; MS 398 (M+),
142 (base peak); IR (KBr) 1621, 1603, 1571, 1557 cm-1.
Analysis calculated for ^25“2°Ν2^3:
C, 75.36; K, 5.57; N, 7.03%.
Found: C, 75.08; K, 5.55; N, 6.87%.
(+)-trans-isomer, recrystallized from CH„Cl^-diiso25 propyl ether, 0.90 g; m.p. 145.5-147° C.; MS 398 (M ),
142 (base peak); IR (KBr) 1619, 1601, 1560 cm X.
Analysis calculated for 1 2o 22 3
C, 75.36; H, 5.57; N, 7.03%.
Found: C, 75.31; H, 5.51; N, 6.89%.
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-164EXAMPLE 277
3S_, 4S.-3- ί 3-Pyridylmethyl) -6- (2-quinoiyl) methoxy4-chrcmanyl Dimethyiglycinate Ester Trihydrochloride
By the methods of Example 240, the 3S,4S-title product of Example 6 (1.0 g, 2.51 mmol) was converted to present title product, recrystallized from ethanolether, 900 mg; m.p. 148° C. (dec.).
Analysis calculated for ConH-,-Cl-,Ν-,Ο. · 0.25H„O:
32 3 3 4 2
C, 58.30; H, 5.48; N, 7.03%.
Found: C, 53.08; H, 5.08; N, 6.94%.
EXAMPLE 278
3S_, 4S.-3- (3-Pyridylmethyl) -6- (2-quinolyl) methoxy-4-chromanol L-Lysine Ester
By the method of Example 117, 3S.,4S-title product of Example 6 (0.50 g, 1.26 mmol) and N(epsilon)-(tbutoxycarbony1)-L-lysine (160 mg, 1.32 mmol) were coupled to form the intermediate N-t-boc protected ester, recrystallized from CHCl^-hexane, 700 mg; m.p. 109-111° C.
Analysis calculated for C.,H_~N.0o:
oO 4 8
C, 67.75; H, 6.93; N, 7.71%.
Found: C, 67.99; H, 7.14; N, 7.77%.
Intermediate prepared in this manner (1.1 g, 1.52 mmol) was dissolved in 100 ml of dioxane saturated with dry HC1, stirred for 12 hours at room temperature, and title product recovered by filtration; exact mass calculated: 526.2580; found: 526.2549.
EXAMPLE 279
3S_, 4S-3- (3-Pyridylmethyl) - 6-(2-cuinolyl) methoxv4-chromanyl 4-ZJiperidinobutyrate Ester Trihydrochloride
By the methods of Example 144, using 1:9 CH^OK:ether as eluant on chromatography of the free base, 3£>,4S-title product of Example 6 (1.0 g, 2.5 mmol) was converted to present title product, isolated as trihydrochloride by
BAD ORIGINAL Ά
-16 5bubbling dry HC1 into a solution of the free base in ether, 331 mg; m.p. 74-73° C.; MS 551 (M+) , 163 (base peak); IR (nujol) 3359, 3372, 2913, 2S65, 1642, 1494, 1457, 1377, 1210, 500, 453 cm1.
EXAMPLE 280
3£5,4S_-3- (3-PyridyImethyl) -6- (2-quinolyl) methoxy-4-chromanol Acetate Ester
To 3S.,4S-title product of Example 6 (0.398 g, 1.0 mmol) in 10 ml CHjClj, stirring under N2, was added 10 ml of acetic anhydride. After stirring for 2 days, the reaction mixture was stripped of volatiles in vacuo and the residue flash chromatographed on silica gel using ethyl acetate as eluant to yield title product as an oil, 385 mg; MS 440 (M+), 142 (base peak). ^H-NMR (DMSO-dg)delta(ppm) 8.45 (d, 1H) , 8.42 (dd, 1H) , 8.38 (d, 1H) , 7.99 (d, 1H) , 7.97 (d, 1H) , 7.60-7.77 (m, 4H) ,
7.30 (dd, 1H) , 6.97 (dd, 1H), 6.87 (d, 1H), 6. 77 (d,
1H) , 5.67 (d, 1H) , 5.25 (AB quartet, 2H) , 4 .09 (dd,
1H) , 3.92 (t, 1H) , 2.51-2.65 (m, 3H) , 1.99 (s, 3H) .
EXAMPLE 231 ( + )-cis-6-{5-Fluoro-2-benzo thiazolyl)methoxy3-(3-(hydroxymethyl) benzv1-4-chromanol
By the method of Example 215, the title product of
Example 50 (2.0 g) was converted to present product, purified without chromatography by recrystallization from THF/ethyl acetate, 1.20 g; m.p. 192-194° C.; exact mass calculated: 451.1253; found: 451.1213.
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-166 J ο
Jl >
EXAMPLE 28 2 (y)-trans-3-(6-Methyl-3-pyridyloxy)-4,6-chromandiol
Above Example 256 was repeated on 6.02 g of Example 255 title product. Following chromatography, the product (4.04 g) was further purified by trituration with 100 ml CH2C12 to yield the (+) -cis-title product of Example 256 (2.54 g). The mother liquor was stripped to yield crude (+)-trans-title product, 0.5 g; tic Rf 0.35 (1:9 CH3OH:CH2C12); 1H-NMR indicates 20% contamina10 tion with the ί-f) -cis-isomer.
EXAMPLE 283 ( +) -trans-3-(6-Methyl-3-pyridyloxy)6-(2-quinolyl)-4-chromanol
Without further purification, the product of the 15 preceding Example (160 mg, 0.59 mmol) was reacted by the method of Example 5 to yield present title product, separated and purified by chromatography on silica gel using gradient elution with 1:99, 1:49, 1:25 and 1:12.5 CH3OH:CH2C12, 113 mg; tic Rf 0.48 (1:9 CH3OH:CH2C12).
EXAMPLE 284
6-Benzyloxy-3-(3-pyrioyloxy)-4-chromanone
To a solution of 3-hydroxypyridi.ne (8.56 g, 90.0 .mmol·) in 300 ml anhydrous DMF was added portionwise 3.6 g of 60% NaH in oil (90 mmol. 1.0 eq.). Stirring
0.5 hour was followed by the addition of 30.0 g (90.0 mmol, 1.0 eq.) of 3-bromo-6-benzyloxy-4-chromanone in one portion. After 1 hour, the reaction was poured into 1 liter Hn0 and extracted 3 x 250 ml ethyl acetate.
z.
The organic layers were combined, washed 1 x 100 mi
H20, 1 x 100 ml 10% LiCi and 1 x 100 mi brine, then dried over Na„3O,. Filtration and solvent removal afforded 40 g crude product. Silica gel chromatography with 1:4 ethyl acetate :CH2C12 as eluant afforded 1.13 g (3.6%) of purified title product; m.p. 133-134° C.
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-167EXAMPLE 235 cis- and trans-6-5enzyloxy-3-(3Ά _pyridyioxy) -4-chrcmanol_
Title product of the preceding Example (2.38 g,
6.85 mmol) was dissolved in 120 ml methanol and 80 ml
THF. After cooling to 0°-5°, NaBH^ (285 mg, 7.54 mmol, 1.1 eq.) was added in one portion. After 75 minutes, the reaction mixture was warmed to room temperature and concentrated in vacuo. Dilution with 600 ml of ethyl acetate was followed by washing 2 x 100 ml H2O and 1 x 100 ml brine. The organic layer was dried (Na2SO^), concentrated and dried to yield 2.4 g of present title products; 1H-NMR(250 MKz, CDCl3)delta 2Hs 5.05 (s, 2H) with small shoulder 5.02 (5¾ by integration). The major compound is cis (95%) and the minor compound is trans (5%). MS 349.0 (M+), 91.0 (base peak).
EXAMPLE 286 cis- and trans-3-(3-Pyridyloxy)4,6-chromandiol
To a solution of the mixed title product cf the preceding Example (6.S2 g) in 150 ml methanol and 75 ml THF was added 2.5 g of 10% Pd/C (50% water wet) and the mixture hydrogenated at 50 psig for 24 hours. Catalyst was recovered by filtration, the mother liquor stripped of solvent, and the residue chromatographed on silica gel using gradient elution with from 1:19 to 1:9 methanol:CH2C12 to yield present title product as a white powder; 4.42 g; MS 295 (M+, base peak).
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-163Γ Υ ’ Μ D Γ Π1 Ο ‘.1 “7 Λ «,-u· 1 r υ ώ Ζ Ο / ( + ) -trann-6- (6-Fluoro-2-cuinolyl} m.cthoxy_3- ί 3-pyridyloxv) -4-chromanone
According to the methods of Example 75, the mixed 5 title products of the preceding Example (2.75 g, 10.6 mmol) were converted to present, chromatographed title product as a 10:1 cis:trans mixture, 3.29 g. Recrystallization from isopropyl ether/CH2Cl2 gave 2.8 g of purified (+)-cis isomer of title product (identical with the product of Example 75). The mother liquor was stripped to yield 1.1 g of a product enriched in transiscmer as an oil, which was chromatographed on silica gel (1:24 CH^CH:CH2C12 as eluant) to yield 0.76 g of a 3:2 cis: trans mixture as a white foam. Final isolation of the title (+)-trans-isomer was achieved using reverse phase hplc, with 40% CH3CN/60% 0.1M NH4OAc (pH 4.3) as the mobile phase, detection at 254 nm, a flow rate of ©
6.3 ml/mmute and a Dupont Zorbax C-8 9.6 mm x 25 cm column as stationary phase. The 3:2 cis: trans mixture was dissolved in 3.3 ml of the mobile phase and injected 0.11 ml on each preparative run. The retention times for the cis and trans-isomers were 15 and 16 minutes, respectively. The product fractions from ten runs were . combined and stripped to yield 60.2 mg of the cis-isomer and present title (+)-trans-isomer, 50.5 mg; m.p.
163-165° C.; MS calculated: 418.1330; found: 418.1214.
EXAMPLE 28 3
6-Methoxy-3-(2- and 3-(trifluoromethyl)phenyl)methylene-4-chromanones
Present title products were each prepared from
6-methoxy-4-chromanone (26.7 g, 0.15 mol) and 2- or 3-(trifluoromethyl)benzaldehyde (29.6 g, 0.17 moi) according to the method of Example 1 to yield 34 g of the 2-isomer (m.p. 130-131° C.) and 23 g of the
3-isomer (m.p. 116-117° C.), respectively.
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-169EXAMPLE 289
3-(2- and 3-(Trifluoromethyl)benzyl6-methoxy-4-chromanones
By the method of Example 2, the title products of 5 the preceding Example were converted to present title products, each triturated with hexane:
2- isomer: 23 g from 33 g; m.p. 72-73° C.; MS 336 (M+) ; IR (KBr) 1687, 1658, 1623, 1609, 1583 cm1.
3- isomer: 9 g from 20 g;
Analysis calculated for
C, 64.29; H, 4.50%.
Found: C, 64.37; H, 4.42%.
EXAMPLE 290
6-Hydroxy-3-{2- and 3-(trifluoro15 methyl)benzyl-4,6-chromanols
By the method of Example 3, the title products of the preceding Example were converted to present title products :
2-isomer: 16.5 g from 21 g; isolated from cyclohexane; m.p. 115-116° C.; MS 322 (iM+) , 136 (base peak); IR (KBr) 1683, 1624, 1610, 1587 cm1.
Analysis calculated for 1 1 / 13 3 J
C, 63.36; H, 4.07%.
Found: C, 63.35; H, 4.08%.
3-isomer: 6.4 g from 9 g; isolated as solid from acetone/hexane; tic Rf 0.23 (8:2:1 hexane:ethyl acetate :diisopropyl ether).
EXAMPLE 291 cis- and trans-3-(2- and 3-(Trifluoro3 0 _methyl)benzyl-4,6-chremandioIs
By the method of Example 4, the title products of the preceding Example were converted to present title products :
ADAΠ 0 0 8 3
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ΜΗ:
)MS( :rom
1647, 162
1603 cm .ncludes delta (ppm) 4.24 :rans mixture; -1 1,.
and 4.39 (bs) , CH-OH, integrated to show the 2:3 cis : trans ratio.
3-isomer: 6 g from 6 g; 9:8 cis: trans mixture;
^H-NMR (same conditions) includes delta (ppm) 4.26 (d, J=6 Hz) and 4.33 (d, J=3 Hz), CH-OH, integrated to show the 9:8 cis : trans ratio.
EXAMPLE 292 (+)-cis- and (+)-trans-6-(2-Quinolyl)methoxya n·'
3-(trifluoromethyl)benzvl)-4-chromanol .χ,
By the method of Example 5, each of the cis-trans mixtures of the preceding Example were converted to chromatographically separated title products:
from 6 g of cis/trans 2-isomer: 1.3 g of (+)-cisisomer; m.p. 119-120.5° C.; MS 465 (M+) ,· IR (KBr) 1621, 1600, 1584 , 1570 cm 3 g of cis-trans mixture suitable for recycling; and 0.4 g of (+)-trans-isomer; m.p. 110-112° C. (from CHCl3/hexane), MS 465 <M+)
1607, 1533, 1569 cm-1.
IR (KBr) cis/trans 3-isome:
tram z.z go (+-) -cis- isomer ; m.p. 149-150
C. (from CH_Cl,/hexane) ;
— -1 2 IR (KBr) 1670, 1617, 1602, 1565 cm ; 3.7 g as cis-tran:
mixture suitable for recycling; isomer; m.p and 0.4 g of trans1617, 155'
147-148° C. (from CH9Cl0/hexane)
£.
524 and 1564 cm
IR (KBr)
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-171EXAMPLE 293
3-(6-Methv1 and 6-methoxv-3-pyridyl)methylene6- ( 2-quinolyl) methoxy-4-chromanones
By the method of Example 1, 6-(2-quinolyl)methoxy5 4-chromanone and the appropriate 6-substituted-3-pyridine carbaldehyde were converted to present title products :
6-methyl analog: 3.38 g from 4.20 g of the chromanone? m.p. 185-186° C. from CH^OH/Ci^C^.
6-methoxy analog: 6,23 g from 7.0 g of the chromanone; m.p. 155-158° C. from CHOH/diisopropyl ether.
EXAMPLE 294
3-(6-Methyl- and 6-methoxy-3-pyridy1)benzyl15 _6-(2-quinolvl)methoxy-4-chromanones_
By the method of Example 2, the products of the preceding Example were converted to present title products :
6-methyl analog: 2.78 g from 3.28 g; m.p.
108-110° C. (from :diisopropyl ether); MS 410 (M+); IR (KBr) 1630, 1637, 1614, 1601, 1584, 1560 cm-1.
6-methoxy analog: 3.71 g from 5.90 g; m.p.
98-99° C. (from Ci^C^: diisopropyl ether); MS 426 (M+) ; IR (KBr) 1686, 1639, 1611, 1571 cm-1.
EXAMPLE 295 ( + ) -cis- and ( + ) -trans-3- (6-xMethyl- and 6-methoxy-3pyridv1)benzyl-6-(2-quinolyl)methoxy-4-chromanols
By the method of Example 5, each of the title products of the preceding Example were converted to present chromatographically separated cis- and transisomers, each isolated from Ci^C^/diisopropyl ether:
from 2.0 g of the 6-methyl analog, 0.516 g of the cis-isomer; m.p. 121-123° C.; MS 412 (M+); and 0.48 g of the trans-isomer; m.p. 164-165° C.; MS 412 (M+).
£ 8 0 0 0 0 dV
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-17210 ·· *
C-.
from 2.4o g of tne metnoxy analog, 1.08 g of the cis-isomer; m.p. 132-133° C.; MS 428 (M+) ; IR (CKC1-.) _ i 3
3589, 3388, 1610, 1571 cm ; and 0.75 g -of the transisomer; m.p. 144-145° C.; MS 428 (M+); IR (CHC1,) 3582, 3374, 1610, 1572 cm1.
EXAMPLE 296
3S,4S-3-(l-Oxo-3-pyridyl)methyl-6(2-quinolyl·)methoxy-4-chromanol
The title product of Example 7 (1.0 g, 2.5 mmol) and m-chloroperbenzoic acid (0.55 g, 3.19 mmol) in 100 ml of CH2C12 was stirred for 12 hours. The reaction mixture was then washed with saturated NaHCO-,, j
dried (MgSO^), stripped of solvent and the residue chromatographed on silica gel using 8:1:1 Ci^Cl^ethyl acetate: isopropanol and finally recrystallized from ethyl acetate and diisopropyl ether to yield purified title product, 0.29 g; m.p. 163-164° C.; exact mass calculated: 414.1487; found:
-65.38° (ethanol).
Analysis calculated fo
414,1579; [alpha]
CocH„_N^O, -0.5H-O:
z2 z 4 2
Founa:
C, 70.91; H, 5.47; N, 6.62% C, 71.01; H, 5.39; N, 6.37%
EXAMPLE 297
Acid Addition Salts of 3S., 4S.-3-(3-?yridylmethyi) 6-(2-quinolyl)methoxy-4-chromanol
Dihydrochlorice
To a solution of 500 mg (1.26 mmol) of the title product of Example 7 in was added, an excess of dichloromet’nane saturated with saturated HCl/CH^Cl^. The solvent was evaporated and the residue recrystallized from ethanol-ether to give 495 mg (84%) of the dihydrochloride salt hydrate; m.p. 135° C. (dec.). Analysis calculated for C_5^^24^^2^2^^3* θ *:
C, 62.51; H, 5.24; N, 5.83%.
Found: C, 62.21; H, 5.17; N, 5.72%.
-17 3ΜΟΠΟ L — T Η Γ '3 Γ Ξ 10
A mixture of 398 mg (l.G mmol) of the title product of Example 7 and 300 mg (2.0 mmol) of L-tartaric acid in 20 ml acetone was heated to obtain a solution and then cooled to 25° C. Pentane (80 ml) was added causing precipitation. The precipitate was collected and recrystallized from acetone-pentane to give 187 mg (34%) of the mono L-tartrate hydrate; m.p. 152-154° C.
Analysis calculated for CoqHooN_0„·1.25H-O:
y 2 o 2 y 2
C, 61.00; H, 5.38; N, 4.91%.
Found: C, 60.66; H, 5.00; N, 4.74%.
Diphosphate
To a solution of 500 mg (1.26 mmol) of title 15 product of Example 7 in methanol was added 0.172 ml (2.51 mmol) of 85% phosphoric acid. The mixture was heated to give a solution and then cooled to 25° C.
The precipitate formed was filtered to yield 390 mg (45%) of the diphosphate salt solvated with one equivalent phosphoric acid; m.p. 148-150° C.
Analysis calculated for C25H28N2°11P2*H3PO4:
C, 43.36; H, 4.51; N, 4.05%.
Found: C, 43.69; H, 4.50; N, 4.05%.
Mono Fumarate
A mixture of 500 mg (1.26 mmol) of title product of Example 7 and 291 mg (2.51 mmol) of fumaric acid in acetone was heated to obtain a solution and then cooled to 25° C. The precipitate formed was collected to yield 500 mg (77%) of the mono fumarate salt; m.p.
165-166° C.
Analysis calculated for C29H26N2°7:
C, 67.69; H, 5.09; N, 5.45%.
Found: C, 67.63; H, 5.04; N, 5.22%.
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EXAMPLE 298
7-Merhoxu-3- (3-pyridyl) methylene-4-chromancna
By the method of Example 1, 7-methoxy-4-chromanone (10 g, 56 mmol) and 3-pyridine carbaicehyde (5.2 g, 73 mmol) were converted to present title product, isolated directly from the reaction mixture by cooling to 0° C., 11.9 g; m.p. 176-178° C.; MS 267 (M+, base peak). Analysis calculated for C^gH^NO^:
C, 71.90; H, 4.90; N, 5.24%.
Found: C, 71.94; H, 4.93; N, 5.05%.
By the sair.e method, 7-methoxy-4-chromanone (5.0 g, 23 mmol) and 3-(methoxycarbonyl)benzaldehyde (2.04 g, 28.7 mmol) were converted to 7-methoxy-3-(3-(methoxycarbonyl) phenyl) methylene-4-chromanone, 6.14 g; m.p.
126-123° C.; MS 324 (M+, base); IR (nujol) 2950, 2913,
2851, 1730, 1661, 1583, 1460, 1292, 1239, 925, 817, s . 288 cm .
j EXAMPLE 299 3 7-Methoxy-3-(3-pyridylmethyl)-4-?hromanone
2C Title product of the preceding Example (12.35 g) in 300 ml CH^OH was hydrogenated for 12 hours at 5C psig over 1.4 g of 10% Pd/C. Catalyst was recovered by filtration. The filtrate was stripped to an oil from which title product was crystallized by trituration with 200 ml of warm isopropyl ether, 9.89 g; m.p,
95-99° C.; MS 269 (M+), 122 (base); IR (CHCl-j) 2953 , 1673, 1611, 1577, 1435, 1258, 837 cn'1.
Analysis calculated for C^gH^.NO^:
C , 71.13; Η , 5.5 7; N, 5.12 % .
Found: C, 70.94 ; H, 5.54; N, 5.06%.
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In like manner the other product of the preceding Example (6.14 g) in 100 ml of 1:1 methanol:THF was converted to 7-methoxy-3-(3-(methoxycarbonyl) ber.zyi4-chromanone, isolated directly upon stripping the catalyst filtrate, 4.50 g; MS 326 (M+, base).
EXAMPLE 300
7-Hydroxy-3-(3-pyridylmethyl)-4-chromanone
By the method of Example 3, title product of the preceding Example (3.8 g) was converted to present title product, 13.2 g; m.p. 181-190° C.
Analysis calculated for ·0.25Η2<0:
C, 69.35; H, 5.24; N, 5.39%.
Found: C, 69.96; H, 5.16; N, 5.33%.
Application of this method to the other product of 15 the preceding Example (4.5 g, 13.8 mmol) concurrently hydrolyzed the methyl ester to yield 7-hydroxy-3-(3carboxybenzyl)-4-chromanone, 1.54 g; m.p. 234-236° C.; MS 298 (M+), 136 (base); IR (nujol) 3316, 2920, 2850, 1706, 1609, 1578, 1448, 1284, 848, 695, 276 cm1. To reesterify, the acid was taken into 34 ml CH^OH, and the solution saturated with dry HC1 and then heated to reflux for 1 hour. The reaction mixture was stripped and the residue recrystallized from CH2C12 to yield the corresponding methyl ester, 1.39 g; m.p. 157-159° C.;
MS 312 (M+), 136 (base); IR (nujol) 2947, 2921, 2348, 1717, 1459, 1376, 1346, 1297, 1248, 1172, 753,
282 cm 1.
EXAMPLE 301
3- (3-Pyridylmethyl)-7-(2-quinolyl)3 0 2_methoxy-4-chroma none_
By the method of Example 5, the title product of the preceding Example (3.0 g, 11.7 mmol) was converted to present title product using in sequence CH2C12, £ 8 0 0 0 0 dV
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Ύ»
..
-176ether and finally 1:19 CH3OH:ether as chromatography eluants to yield present, purified title product,
2.17 g; m.p. 111-114° C.; IR (nujol) 2950, 2921, 2851, 1677, 1611, 1243, 1173, 834, 267 cm-1.
In like manner, the other, esterified product of the preceding Example (700 mg, 2.24 mmol), was converted to chromatographically purified 3-(3-(methoxycarbonyl) benzyl-7- (2-quinolyl)methoxy-4-chromanone,
394 mg; MS 453 (M+), 142 (base); IR (CHC13) 2947, 1721, 1607, 1466, 1436, 1287, 1256, 1237, 1163, 822, 218 cm-1.
Substituting 2-(chloromethyl)-6-fluorobenzothiazole for 2-(chloromethyl)quinoline, the other, esterified product of the preceding Example (1.63 g, 5.22 mmol) was converted to 7-(6-fluoro-2-benzothiazolyl)methoxy3-(3-(methoxycarbonyl)benzy1-4-chromanone, 1.5 g; m.p. 162-165° C.; IR (nujol) 2946, 2926, 1723, 1457, 1366, 1276, 1156, 965, 800, 748, 266 cm1.
EXAMPLE 302 (+)-cis- and (+)-trans-3-(3-Pyridylmethyl)7-(2-quinolyl)methoxy-4-chromanol
By the method of Example 4, the title product of the preceding Example (1.97 g, 5.0 mmol) was converted to present title products, separated by chromatography on silica gel using ether and then 1:19 CH3OH:ether as eluants to yield title products as follows:
( + )- cis-product: 944 mg; m.p. 48-65° C.; MS 398 (M+), 142 (base); IR (CnCl^) 2948, 1617, 1265, 1163, 723 cm1.
Analysis calculated
C, 75.36; H Found: C, 75.08; H for C
2o 22
5.56; N, 7 5.41; N, 6 .03%.
.81%.
( + )-trans-product: 680 mg; m.p. 53-55* C.; MS 398 (M+) , 131 (base); IR (CHC1,) 2954, 1618, 1425, 1270,
-1 *
1162, 249 cm .
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-173PREPARATICN 1 4- (2-Cyanoethoxv) anisole
4-Methoxyphenol (248 g) , KOH (5.6 g) and acrylonitrile (397 ml) were dissolved in 1 liter of t-butanol and heated with stirring at 75° C. for 5 hours. The mixture was then cooled to room temperature and stripped in vacuo to solid residue, which was repulped in ether and insolubles recovered by filtration. The latter were taken up in 2 liters of ethyl acetate, washed in sequence with 1 liter each of H^O, saturated NaHCO^ and saturated NaCl, dried over MgSO^ and restripped to yield purified title product, 199.4 g, m.p. 62-64° C.
PREPARATION 2 6-Methoxy-4-chromanone
The title product of the preceding Example (199 g) was combined with 240 ml H^O and 480 ml of concentrated HC1 and heated at reflux overnight. The reaction mixture was cooled to room temperature and solids recovered by filtration. The latter were taken up in 2 liters of ethyl acetate, washed with 200 ml of H^O, dried over
McSO. and stripped in vacuo to yield intermediate 4 * *
3-(4-methoxyphenoxy) propionic acid, 195 g, m.p.
105-107° C. The latter was added to 600 ml of hot, stirred polyphosphoric acid maintained at 75° C.
and the mixture stirred for 2 hours. The temperature rose to a maximum of 89’ C. over the first one-half hour, then fell to the 75° C. bath temperature. The reaction mixture was quenched into 3.2 liters of ice and water and extracted with 1.2 liters of ethyl acetate. The organic extract was in sequence with 600 ml each of K20, saturated NaHCO^ and saturated NaCl, dried over MgSO^ and stripped to ISO g of solids which were taken up in 400 ml CH2C1-, treated with activated carbon and restripped to a like quantity of
ΛΡΠn 0 0 8 3
BAD ORIGINAL solids. The latter were recrystallized from isopropyl ether to yield purified title product, 120 g, m.p. 46-43° C., identical with the commercial product.
PREPARATION 3 6-Hydroxy-4-chromanone
A solution of 36 g of the product of the preceding Preparation in 290 ml of acetic acid and 290 ml of 48% hydrobromic acid was heated at reflux for 3 hours. The reaction was cooled and stripped in vacuo to crude product which was diluted with water (6 liters), cooled to 0-5° C. and title product recovered by filtration, 25.7 g (80%), m.p. 133-136° C. Optionally, the product is further purified by chromatography on silica gel using ethyl acetate/hexane as eluant.
PREPARATION 4
A*
6-Benzyloxy-4-chromanone
A mixture of 25 g of the product of the preceding Preparation, 26.5 g of benzyl bromide and 28 g of potassium carbonate in 150 ml of acetone was heated at reflux overnight. The reaction was cooled and filtered to remove potassium carbonate. The filtrate was evaporated and the residue was dissolved in ethyl acetate and washed with water. The ethyl acetate layer was dried over sodium sulfate and evaporated in vacuo to obtain the crude product, which was purified by recrystallization from methylene chloride/hexane to give 29 g of title product, m.p. 107-108° C.
^H-NMR(acetone-d-) delta(ppm) : 2.7 (t, 2H) , 4.4 (t,
2H) , 5.08 (s, 2H) , 7.2-7.5 (m, 3H) .
BAD ORIGINAL
-131PREPARATION 5
3-Hydroxyme thylene-6-benzy loxy-4-chroma ncr.e
To a solution of 172.5 g of the product of the preceding Preparation in 1.7 liters of toluene contain5 ing 168 ml of ethyl formate and 3.5 ml of ethanol was added, in portions, 66 g of 50% sodium hydride. The reaction was allowed to stir at room temperature for 1 hour, then poured into 1.5 liters of ice and H2O, and acidified to pH 4 with dilute hydrochloric acid. The aqueous layer was extracted with several portions of ethyl acetate. The organic layers were combined, dried over sodium sulfate and evaporated in vacuo to give the crude product which was triturated with hexane to remove hydride oil. The resultant product crystallized on standing, m.p. 82-85° C.
PREPARATION 6
3-Diazo-6-benzyloxy-4-chromanone To a -10° C. solution of 35.3 g of title product of the preceding Preparation in 250 ml of dichloromethane containing 25.2 g of triethylamine was added dropwise a solution of 24.4 g of tosyl azide in 100 ml of cichlorcmethane. After complete addition, the reaction was allowed to warm to room temperature and stirred overnight. The reaction mixture was washed with water, dried over sodium sulfate and evaporated in vacuo to give the crude product, which was purified by column chromatography on silica gel eluting with dichloromethane to give 21 g of product, m.p. 100-103° C.
1H-NMR(CDC13)delta(ppm): 5.02 (d, J=4, 2K), 6.7-7.5 (m, 10H) .
Bau umIGINAL
AP 0 0 0 0 8 3
-18 2PREPARATION 7
4-(4-Methoxyphenoxy)butyric Acid 4-Methoxyphenol was added to a solution cf NaOC^H^ made by dissolving 2.3 g of Na in 50 ml ethanol. After 5 5 minutes, gamma-butyrolactone was added and the mixture heated at reflux overnight. Ethanol was distilled off and the residue heated at 155° C. overnight, then cooled, diluted with water and acidified to pH 3 with dilute hydrochloric acid. The product was collected by filtra10 tion, 19.5 g, m.p. 103-104° C.
PREPARATION 8
3,4-Dihydro-7-methoxy-l-benzoxepin-5(2H) -one
The product of the preceding Preparation, 34 g, was dissolved in 300 ml of polyphosphoric acid and heated at 100° C. for 1 hour. The reaction was cooled, poured into water and extracted with ether to give the crude product. It was purified by distillation, b.p.
100° C./0.5 mm.
PREPARATION 9
3 , 4-Dihydro-7-hydroxy-l-benzoxepin-5(2H) -one
A mixture of 19.23 g of the product of the preceding Preparation, 95 ml of 48% hydrobromic acid and 95 ml of acetic acid was heated at reflux for 4 hours. The reaction was cooled and evaporated in vacuo to afford the crude product, which was purified by column chromatography on silica gel, eluting with dichloromethane to give 8.3 g of product, m.p.
116-120° C.
1H-NMR(CDCi3)delta(ppm) : 2.0-2.45 (m, 2H) , 2.95 (t,
J = 7 , 2H), 4.20 (t, J=7 , 2K), 6.8-7.1 (m, 3H), 7.4 (s,
1H) .
BAD ORIGINAL ft
-133PREPARATION 10
7-Senzyloxy-3 , 4-dihydro-i-benzoxepin-5 ( 2H -one
A mixture of 6.5 g of the product of the preceding Preparation, 4.3 ml of benzyl bromide, 6.3 g of potassium carbonate and 40 ml of acetone was heated with stirring at reflux overnight. The reaction was cooled and filtered to remove inorganics. The filtrate was evaporated in vacuo, and the residue dissolved in ethyl acetate and washed with water. The ethyl acetate layer was dried over sodium sulfate and evaporated in vacuo to give the crude product which was purified by recrystallization from isopropyl ether to give 8.4 g of title product, m.p. 62-63° C.
PREPARATION 11
7-Benzvloxy-4-bromo-3,4-dihydro-l-benzoxepin-5(2H)-one
To a solution of 6.3 g of the title product of the preceding Preparation in 25 ml of acetic acid was. added a solution of 3.76 g of bromine in 25 ml of acetic acid. The reaction was stirred for 3 minutes and the volatiles evaporated in vacuo to a residue which was dissolved in ethyl acetate and washed with water. The ethyl acetate layer was dried and evaporated to give 8.2 g of product which was used without purification in the next step.
PREPARATION 12
3-3rcmo-6-methoxy-4-quinolone
To a solution of 6-methoxy-4-chrcmanone (35 g) in ethyl ether (1.6 liters) at 5-10° C. was added dropwise over 30 minutes 10.6 ml of bromine. The mixture was stirred at 5-10° C. for 30 minutes and then allowed to warm to room temperature. After 2 hours tic (Cl^C^) indicated formation of less polar products and only a trace of starting material remaining. The reaction mixture was washed with water (1 liter), saturated
ΔΟ n ft 0 0 8 x
BAD ORIGINAL d
-χότ
NaHCO^ (500 ml), and brine (SCO ml), dried over MgSO^, and concentrated in vacuo to a yellow solid. The crude product was purified by silica gel flash column chromatography on 2.4 Kg fine silica gel, eluting with a gradient system consisting of 3:1 hexanes/dichloromethane followed by 2:1 hexanes/dichloromethane and finally 30% hexanes/dichloromethane. This afforded title product as a yellow solid in 80% yield.
PREPARATION 13
1-Amino-5-methylcyclohex-l-en-3-one
5-MethyI-l,3-cyclohexanedione (40 g, 0.32 mol) was dissolved in 500 ml of benzene at 70° C. The solution was heated at reflux for 2 hours, during which NH^ was bubbled through the reaction mixture and formed r^O was collected in a Dean-Stark trap. The mixture was then cooled to 0° C. and title product recovered by filtration, 39.8 g, m.p. 165-169° C.
^H-NMR(DMSO-άθ) delta(ppm): 0.98 (s, 3H) , 1.6-1.38 (2H), 2.14-2.38 (2H), 3.14-3.6 (1H), 4.93 (s, IK),
6.2-7.2 (m, 2H).
PREPARATION 14
7,8-Dihydro-7-methy1-3-nitro-5(6 K)-quinolone Sodium nitromalonaldehyde (Org. Synth. Coll., vol.
4, p. 844; 42.4 g, 0.269 mol) was dissolved in 200 ml of dimethyiformamide and the resulting solution dried over 4A-type molecular sieves, recovered by filtration with 100 mi of the same solvent for wash. To the combined filtrate and wash was added pyridine (91 mi, g, 1.13 mol) and the mixture cooled to -5° C. Tosyl chloride (53 g, 0.277 mol) in 200 ml of dimethy1 formamide was added dropwise, maintaining a temperature of -5° to -8° C., and the reaction mixture allowed to warm to room temperature. The title product of the preceding Preparation (33.6 g, 0.270 mol), dissolved by warming
BAD ORIGINAL
-185in 200 ml of dimethy1 formamide and added in a steady stream to the reaction mixture, which was then stirred for 13 hours at room temperature, then poured into 2 liters of ice and water and extracted 2x1 liter of ethyl acetate. The organic layers were combined, dried over MgSO4 and stripped to yield present title product, g (61%), m.p. 64-67° C.
PREPARATION 15
3-Amino-7,8-dihydro-7-methyl-5(6H)-quinolone 10 Title product of the preceding Preparation (27 g) was placed in. a 250 ml Parr bottle with 830 ml absolute ethanol· and 9.0 g 10% Pd/C. This was then agitated on a Parr apparatus under 50 psig H2 for 2 hours at room temperature. The catalyst was recovered by filtration over diatomaceous earth and the filtrate was concentrated to dryness. The resulting brown solid was flash chroma- <x tographed by first dissolving in CH^OH, adding 50 ml C dry 32-63 micron silica gel and concentrating to dryness.
The resulting material was then charged dry onto a c
30 cm x 15 cm column of fresh silica gel which had been wet packed with 1% triethylamine in 19:1 CH2Cl2:isopropanol. The column was eluted with the same solvent system. Middle product-containing fractions were combined and stripped to yield present title product, M5 (m/e) calculated: 176.0950, found: 176.0944; tic (19:1 CH2C12:C2H5OK) Rf 0.32.
PREPARATION 16
7,8-Dihydro-7-methvl-5(6K)-quinolone6-diazonium Hexafluorophosphate
At room temperature, title product of the preceding Preparation (15.26 g) was placed in a 500 ml 3-necked flask equipped with a mechanical stirrer, dropping funnel and venting line placed up the back of the fume hood. Then 6.93 ml glacial acetic acid was added. 159 Ml of 3.48N HC1 was then added all at once
BAD ORIGINAL $ ·- . I — tie n
J
J b
:1 ion mmturc reci i tate ' go.ic precipitates cut o: solution. .
t 0° C., was then added 5.98 g NaNO^ in )5 ml H2O, dropwise over 5-10 minutes, and the resulting .“j mixture stirred at 0° C. for 30 minutes. Still maintaining 0° C., 15.24 ml HPFg over 5 minutes. A light brown precipitate formed immediately. Vigorous stirring was continued for 10-15 minutes after addition was complete. The resulting solid was filtered, washed with 2x25 ml cold n90, 2x25 ; ether and then dried under high vacuum overnight over P?Gr to yield 25.62 g (39%) of present title product, mlpt 175-176.5° C.
PREPARATION 17
7,8-Dihydro-3-hydroxy-7-methyl-5(6H) -guinolcne
Title product of the preceding Example (25.62 c) was added in 0.5 g portions to 500 ml of boiling 5% (60 weight % in H2O) was added ;ver a time period (2.5 hours in this instance) :d excessive foaming due to h’2 evolution. The reaction mixture was beared at reflux for an add! tional 40 minutes, then cooled to 0° C. and adjusted oh 7 with SN NaCH (160 mi required in this instance) . The reaction mixture was extracted 3x250 ml ethyl acetate. in the first extraction, the emulsion was broker, bv filtration over diatomaceous earth. The organic extracts were combined, dried over MgSC_, , stripped to r
S 1 '3 1* 1* - β C WiC?. olilCd C’G crooned cis - prcCGGiSc usin<j 19:1
CE2C12:isopropanol as eluant to yield present t product, 9.2 g (67%) , m.p. 210.5-212° C.
:O .
wmen avo ids, and the residue dissolved in CH,Cr:,
BAD ORIGINAL ά r~ι
PREPARATION 18
3-Benzy loxy-7,8-dihydro-7-methyl-5 (6H) -qu inolone
By the method of Preparation 4, the product of the preceding Preparation was converted to present title product in 78% yield, m.p. 80.5-81.5° C. MS (m/e) calculated: 267.1259, found: 267.1261.
PREPARATION 19
2-Chloromethylquinoxaline
2-Methylquinoxaline (8.94 g) was combined with
50 ml CC14 and 6.5 g Na2CO3 in a 125 ml beaker. This was heated to 68° C. and then Cl2 was introduced via an inverted funnel so that the Cl2 was bubbled very slowly. This was continued for 1 hour and then the reaction mixture was cooled to 20° C. in an ice bath and parti15 tioned between ether and saturated NaHCO^ solution.
The ether was separated, dried over MgSO^, and concentrated to dryness. The residue was immediately flashed down a column packed with 20 cm of 32-63 micron silica gel (the column having a diameter of 8 cm) using 1:1 ether: hexane as eluant. After a 1 liter forerun,
250 ml fractions were collected. Fractions 3-5 were combined and concentrated to yield 2.58 g (23%) of title product as a yellow solid; tic (3:7 ethyl acetate : CH2C1-,) Rf 0.65.
1H-NMR(CDC13)delta(ppm): 4.86 (s, 2n), 7.74-7.78 (m,
2H) , 8.02-8.16 (m, 2K), 9.0 (m, 1H).
ADft ft ft 0 ft 3
BAD ORIGINAL £
PREPARATION 20
- Βromp -3 ,4 -ΰ rnydrc-7-methoxy-1 ( 2Η) -ηaphthalenone
To a 10° C. solution of 25 ς (0.142 moi) of 7-methoxy-3,4-dihydro-l (2H)-naphthaler.or.e in 1 liter ether was added dropwise (maintaining reaction temperature at about 10° C.) 37.9 g (0.237 mol) of bromine. The reaction solution was concentrated on a rotating evaporator and the residue crystallized from ether to give 31.6 g (87%) of present title compound, m.p. 79-80° C.
MS (m/e) 256 and 254 (M ), 174, 173, 143, 131
120 , 1 and 103.
Ir (CHC13) 1680, .610 cm
H-NMR(CDCi3)delta(ppm): 2.2-2.7 (m, 2K), 2
2H) , 3.95 (s, CCH3), 4.78 (t, J=4 Kz, CH3r), (m, 2ArH) and 7.58 (bs, ArH).
Analysis calculated for C^^H33BrO2· iH2O:
C, 50.89; H, 4.46%.
Found: C, 50.71; H, 4.36%.
PREPARATION 21
6-Benzy loxy-3-methyler.e-4-chroma none A solution of 9.2 g of 6-benzyloxy-4-chromanone, dimet'ny lam ine hydrochloride and 1.3 g of paraformaldehyde in 100 ml of acetic acid was heated on a steam bath for The r nours.
volatiles were evaporated i:
9-3.5 7.0-7, (m, vacuo ana the residue was purified on silica gel, eluting with Rf (CH->C1_) = 0.5.
(s, 2H), (s, 1H) , 6.30 (s, 1H) , 6.80-7.60 (m, SH) .
CH^Cl^, to give 3.7 g of product, 1H-NMR(CDC13)delta(ppm): 4.95 (s, 2H), 5
BAD ORIGINAL
-139PRSRARATICN 22
3-Sromo-2-(bromomethyl)-6-methyl pyridine and
3-3romo-6-(bromomethyl·)-2-methyl pyridine 5 To a 25 ml round bottomed flask equipped with a stir bar and condenser, under an inert atmosphere, were added 1.4 g (7.35 mmol) of 3-bromo-2,6-lutidine, 1.21 g (6.77 mmol) of N-bromosuccinimide, 4.5 ml of carbon tetrachloride, and 10 mg (0.04 mmol) of benzoyl peroxide.
The resulting mixture was refluxed overnight. Tic at this point indicated that there still was starting material present, so 0.7 g (3.9 mmol) of N-bromosuccinimide was added and the reaction mixture refluxed for an additional 4 hours. The precipitate was filtered off and washed 2x50 ml CCl^ (hot). The filtrate was concentrated to an oil and the crude product was then purified by flash chromatography on 200 g silica gel with 3:1 hexane:CH2C12 as eluant to yield the two title compounds, 218 mg (11%) yield of the 2-(bromomethyl) derivative and 285 mg (14%) yield of the 6-(bromomethyl) derivative, tic (3:1 hexane:CH2Cl2) Rf 0.07 and 0.13, respectively.
2-(bromomethyl) derivative.
1H-NMR(DMSO-dg)delta(ppm): 7.99 (d, J=7.3 Hz , 1H) ,
25 7.19 (d, J=7.8 Hz, 1H), 4.71 (s, 2H) , 2.46 (s, 3H) .
6-(bromomethyl) derivative.
^H-NMR(DMSO-dg) delta(ppm) : 8.00 (d, J=7.8 Hz , 1H) ,
7.32 (d, J=7.8 Hz, 1H), 4.63 (s, 2Kz ), 2.56 (s , 3K)
non η ο o a 3 bad original »' i ι-~ -........’ ·. r '--o'·,. j. j.,ι i— 1 ets g , 0.1 ; ~ mo — ) , uaklui.··^ if under hexane, wore added to 540 ml dry methanol, under Following dissolution, the mixture 'was diluted with 900 ml methanol, 4-nitro-2-methylpyridine N-oxide (26.0 g, 0.169 mol) was added, and the mixture heated at reflux for 1 hour, cooled to room temperature and acidified with 13 ml glacial acetic acid. .After stirring 15 minutes, the reaction mixture was strapped - salvent, the orange residue taken up in 300 ml of do ., n .uuralized with saturated NaHCO-, , rotor.J) centrated to dryness, and the residue triturated 5 x 50 mi oi ethanol. The ethanol triturates were combined, stripped to dryness, and the residue restripped 3 x 50 ml toluene to yield solids (36.1 g) which were chromatographed on silica gel using 6:1 CH-C1-, :CH-,OK as eluant to yield purified title product, 21.14 a; MS 139
JVD
Y'iii, /r 're; o i tr.a pr-ccri ^reparation /21.14 c) was radijx.ed in 1C J ml of acetic ankvdri ;.· was taken -p 440 ml of water and extracted 4
100 ml of Css'4-. The organic layers were combined, dried (ba-..itd . s slipped and the residue oil chromatographed on silica gel gradrontly eluted with 3:2, 7:1
0 orc duo t as a.. si, 2 0 . 7 b g; tic Rf 0.9 ( 6 : 1 Ch „C s ~ : C h -Oft:) ;
ht 111 id-'' .
BAD ORIGINAL
-191PREPARATION 25
- H y1roxymethyl-4-methoxypyridine Title product of the preceding Preparation (20.75 g, 0.114 mol) and sodium methoxide (9.23 g,
0.171 mol) were combined in 110 ml methanol and heated under reflux for 65 minutes. The mixture was cooled, stripped in vacuo, the residue taken up in 100 ml H2O, neutralized with IN HCl and extracted with 3 x 70 ml of ethyl acetate. The organic layers were combined, dried (Na2SO^) and stripped to yield title product as a solid, 14.2 g.
PREPARATION 26
4-Methoxy-2-picoly1 Chloride To title product of the preceding Preparation (500 mg, 3.6 mmol) in 6 ml CH2C12, stirring under N2, was added dropwise 0.26 ml (3.6 mmol) of SOC12 over 5 minutes, then stirred for 20 minutes, combined with 20 ml H20, neutralized with saturated NaHCO^ and extracted with 25 ml CHCl^. The organic layer was dried (MgSO^) and stripped at low temperature to yield title product as an oil, 494 mg, used immediately in the next chemical step.
PREPARATION 27
6-Benzyloxy-3-bromo-4-chromanone By the method of Preparation 12, 6-methoxy-4chromanone (200 g, 0.79 mol) was converted to present title product, purified by chromatography on silica gel gradiently eluted with 1:1, 2:1, 3:1 and 1:0 CH2C12:hexane, recrystallizea from isopropyl ether, 41.4 g, tic Rf 0.4 (CH2C12).
ADfl n 0 0 8 3 bad ORIGINAL $
-1923-Hydroxy-2-methyIpyr id me 2-Acetylfuran (21.2 g) , concentrated NH^CH (325 ml) and Ii2O (175 ml) were combined and heated in 5 an autoclave at 150° C. (observed pressure, 262 psig) for 22 minutes. The mixture was cooled, stripped and the residue chromatographed on silica gel eluting sequentially with 1:19 and then 1:9 CH3OH:CH2C12 to yield 13.2 g of title product. Recrystallization from isopropanol gave 9.68 g of purified title product in two crops; tic Rf 0.3 (1:9 CH^OH:CH2CI2).

Claims (10)

1. A racemic or optically active compound having the structural formula wherein n is 0 or 1;
m is 0 or an integer from 1 to 3;
X is CH2, 0, S, SO, S02, NH or N (C^-C^) alky1;
XI is CH2, 0, S, SO or S02;
Y and Y1 are taken together and form a carbonyl group, or Y and Y1 are taken separately, Y is hydrogen and Y1 is hydroxy or an acyloxy group which is hydrolyzed to form a hydroxy group under physiological conditions;
Z is CH2, CHCH3, CH2CH2 or CH2CH2CH2;
Z1 is CH or N;
R is 2-, 3- or 4-pyridyl, 2-, 3-, 4- or 8-quinoiyl,
1-, 3- or 4-isoquinolyl, 3- or 4-pyridazinyl, 3- or
4- cinnolinyl, 1-phthalazinyl, 2- or 4-pyrimidinyl, 2or 4-quinazolinyl, 2-pyrazinyl, 2-quinoxalinyl, 1-, 2or 3-indoliziny1, 2-, 4- or 5-oxazolyl, 2-benzoxazolyl, 3-, 4- or 5-isoxazolyl, 5-benzo[c]isoxazolyl, 3-benzo[d]isoxazolyl, 2-, 4- or 5-thiazolyl, 2-benzothiazoly1,
3-, 4- or 5-isothiazolyl, 5-benzo[c]isothiazolyl,
3-benzo[d]isothiazolyl, 1-[(C^-Cj)alkyl]-2-, 4- or
5- imidazolyl, 1-[(C^-C^) alkyl]-2-benzimidazolyl,
1-[ (C^-C^j)alkyl]-3-, 4- or 5-pyrazolyl, 2-[ (C^-C^) alkyl]3 (2K)-indazolyl, or 1-[ (C^-C^,) alkyl]-3 (1H)-indazolyl; or one of said groups mono- or disubstituted on carbon with the same or different substituents which are bromo,
ΔΡ 0 0 0 0 8 3
BAD original
-194' Qfi ehloro, fluoro, (C-^-C^) alkyl, trifluoromethyl, hydroxy, hydroxymethyl or (C^-C^)alkoxy, or on adjacent carbons with trimethylene, tetramethylene, -CH_-O-CH^- or
-O-CH--O-;and 21
R is attached by means of aromatic or heteroaromatic carbon and is phenyl, naphthyl, pyridyl, quinolyl, isoquinolyl, pyridazinyl, cinnolinyl, phthalazinyl, pyrymidinyl, naphthyridinyl, pyrrolyl,
N-[(C^-C^)alky1]pyrrolyl, indolyl, N-[(C^-C^)alkyl]indolyl, isoindolyl, N-[(C^-C^)alkyl]isoindolyl, indolizinyl, pyrazolyl, 1-[(C^—C4)alkyl]pyrazolyl, indazolyl, 1-ί(C-C ,) alkyl]-lK-incazolyi, 2—{(C,—C.) — alkyl]-2H-indazolyl, imidazolyl, 1-[(C^-C^) alkyl]imidazolyl, benzimidazolyl, 1-[(C^-C^) alkyl]benzimidazolyl, furyl, benzofuranyl, isobenzofuranyl, oxazolyl, benzoxazolyl, isoxazolyl, benzo[c]isoxazolyl, benzo[d]isoxazolyl, thienyl, benzothiophenyl, isobenzothienyl, thiazolyl, benzothiazolyl, isothiazolyl, benzoic]isothiazolyl, or benzo[d]isothiazolyl; or, only when either X1 is CH. or m is at least 2, R1 is attached by means of heterocyclic nitrogen and is 1-pyrrolyl, 1-indolyl,
2-isoindolyl, 1-pyrazolyl, 1{IH)-indazolyl, 2(2K)indazolyl, 1-imidazolyl or 1-benzimidazolyl; or R1 is one of said groups which is mono- or disubstituted on carbon with the same or different groups which are bromo, chloro, fluoro, hydroxy, hydroxymethyl, (C^-C^ alkyl, (C^-C^) alkoxy, carboxy, [(C^-C^) alkoxy]carbonyl, or substituted on adjacent carbons with trimethylene, tetramethylene, -CH0-G-CH0- or -O-CH-,-0-; or substituted on tertiary nitrogen to form an N-oxide;
a pharmaceutically acceptable acid addition salt thereof; or a pharmaceutically acceptable cationic salt when the compound contains a carboxy group.
BAD OHlGlNAL
-1952. A compound of claim 1 wherein Y and Y1 are taken together and form a carbonyl group.
3. A compound cf claim 1 wherein Y and Y1 are taken separately, Y is hydrogen and Y1 is an acyloxy group in which the acyl moiety is the alpha-aminoacyl residue of a naturally occurring L-alpha-amino acid,
0 0 0 -C-(CH2)pNR2R3, -C-CHNH2(CH2)qNR2R3, -C-(CH2)rCOOH, or
-C-CHNH2(CH9) COOR;
2 3
R and R are taken separately and are each
2 3 independently hydrogen or (C^-C^) alkyl, or R and R are taken together with the nitrogen to which they are attached to form a pyrrolidine, piperidine, perhvdroazepin or morpholine ring;
p is an integer from 1 to 4;
q is an integer from 1 to 3;
r is an integer from 2 to 3; and s is an integer from 1 to 3.
4. A compound of claim 1 wherein Y and Y1 are taken separately, Y is hydrogen and Y1 is hydroxy.
5. A compound of claim 4 wherein n is 1, m is 0,
X and X1 are each independently CH2 or 0, Z is CH2, Z1 is CH, R is 2-, 3- or 4-pyridyl, 2-quinolyl,
6-fluoro2- quinolyl, 5-fluoro-2-benzothiazolyl or 2-pyrazinyl, and R1 is phenyl, 3-methoxyphenyl, 4-methoxypheny1,
3- methoxycarbonylphenyl, 4-methoxycarbonylphenyl, 3-carboxyphenyl, 4-carboxyphenyl, 2-pyridy1 or 3-pyridyl.
ap η n 0 0 8 3
BAD ORIGINAL ft
-1 9 όβ. A racemic or optically active ccmpc; claim 5 having the relative stereochemical fc ot
7. A compound of claim 6 wherein X is Ο, X1 is CH^, R is 2-quinolyl or 5-fluoro-2-benzthiazoiyl and R1 is 3-pyridyl or 3-carboxyphenyl.
3. An optically active compound of claim 5 having the absolute stereochemical formula
-νΤ
OH .7*
Ο
9»
9. A compound of claim 8 wherein X is 0, X-1 is CH2, R is 2-quinolyl or 5-fluoro-2-benzthiazoiyl and R1 is 3-pyridyl or 3-carboxyphenyl.
10. A racemic or optically active compound having the structural formula
R
R b
c wherein n is 0 or 1;
X is CH_, 0, S, SO, SO , NH or N(C.-C^)alkyl; 1 z z
Σ is CH or N;
BAD ORIGINAL &
-197in the first alternative 2 3
Y and Y are taken together and form a carbonvl group, or Y2 and Y2 are taken separately, Y2 is 3 hydrogen and Y is hydroxy; and Ra is hydroxy or benzyloxy;
R1 5 and Rc are taken separately and R13 is hydrogen and RC is -X1-(CHO) -R1;
z m m is 0 or an integer from 1 to 3;
X1 is CH2, 0, S, SO or S02; and
R1 is attached by means of aromatic or heteroaromatic carbon and is phenyl, naphthyl, pyridyl, quinolyl, isoquinolyl, pyridazinyl, cinnolinyl, phthalazinyl, pyrymidinyl, naphthyridinyl, pyrrolyl,
N-[(C1-C4)alkyl]pyrrolyl, indolyl, N-[(C^-C4)alkyl]indolyl, isoindolyl, N-[(C^-C^) alkyl]isoindolyl, indolizinyl, pyrazolyl, 1-[ (C^-C^)alkyl]pyrazolyl, indazolyl, 1-[(0^-04) alkyl]-lH-indazolyl, 2-[(C3~C4)alkyl]-2H-indazolyl, imidazolyl, 1-[ (C3~C4) alkyl]imidazolyl, benzimidazolyl, 1-[ (C^—) alkyl]benzimidazolyl, furyl, benzofuranyl, isobenzofuranyl, oxazolyl, be.nzoxazolyl, isoxazolyl, benzo[c]isoxazolyl, benzo[d]isoxazolyl, thienyl, benzothiophenyl, isobenzothienyl, thiazolyl, benzothiazolyl, isothiazoly1, benzo[c]isothiazolyl, or benzo[d]isothiazolyl; or, only when either X1 is CH2 or m is at least 2, R1 is attached by means of heterocyclic nitrogen and is 1-pyrrolyl, 1-indolyl, 2-isoindolyl, 1-pyrazolyl, 1(1H)-indazolyl, 2(2H)indazolyl, 1-imidazolyl or 1-benzimidazolyl; or R1 is one of said groups vzhich is mono- or disubstituted on carbon with the same or different groups which are bromo, chloro, fluoro, hydroxy, hydroxymethyl, (C^-C4) alky1, (C3~C4)alkoxy, carboxy, [(C3-C4) alkoxy]carbonyl, or substituted on adjacent carbons with trimethylene, tetramethylene -CH2-C-CH2- or -0-CH2~0-; or substituted on tertiary nitrogen to form an N-oxide;
ΔΡ0 0 0 0 8 3
BAD ORIGINAL ft υ»
-193or in the second alternative
Y and YJ are taken together and form a carbonyl group;
R^ and RC are taken together and are hydroxyb c methylene or diazo; or R and R are taken separately, b c
R is hydrogen and R -is bromo;
Ra is ; and
R6 is phenyl, 2-, 3- or 4-pyridyl, 2-, 3-, 4- or 8-quinolyl, 1-, 3- or 4-isoquinolyl, 3- or 4-pyridazinyl,
3- or 4-cinnolinyl, 1-phthalazinyl, 2- or 4-pyrimidinyl, 2- or 4-quinazolinyl, 2-pyrazinyl, 2-quinoxalinyl, 1-,
2- or 3-indolizinyl, 2-, 4- or 5-oxazolyl, 2-benzoxazolyl, 3-, 4- or 5-isoxazolyl, 5-benzo[c]isoxazolyl,
3- benzo[d]isoxazolyl, 2-, 4- or 5-thiazolyl, 2-benzothiazolyl, 3-, 4- or 5-isothiazolyl, 5-benzo[c]isothiazolyl, 3-benzo[d]isothiazolyl, 1-[(C^-C4) alkyl]-2-, 4or 5-imidazoly1, 1-[(C^-C^) alkyl]-2-benzimidazolyl,
1-[(C1~C4)alkyl]-3-, 4- or 5-pyrazolyl, 2-[(C1~C4)alkyl]3(2H)-indazolyl, or 1-[ (C^-C^ alkyl]-3 (1H)-indazolyl; or one of said groups mono- or disubstituted on carbon with the same or different substituents which are bromo, chloro, fluoro, alkyl, trifluoromethyl, hydroxy, hydroxymethyl or (CL-C^) alkoxy, or on adjacent carbons with trimethylene, tetramethylene, -CU^-O-C'd^- or -O-CH2-O-.
11. A pharmaceutical composition for administration to a mammal which comprises a 5-lipoxygenase inhibiting and/or leukotriene D4 receptor blocking amount of a compound of claim 1 and a pharmaceutically acceptable carrier. *
APAP/P/1988/000106A 1987-10-19 1988-10-17 Substituted tetralins, chromans and related compounds in the treatment of asthma, arthtitits and related diseases. AP83A (en)

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US5298512A (en) * 1989-04-07 1994-03-29 Pfizer Inc. Substituted chromans and their use in the treatment of asthma, arthritis and related diseases
WO1990015801A1 (en) * 1989-06-22 1990-12-27 Pfizer Inc. Substituted sulfonamides and related compounds in the treatment of asthma, arthritis and related diseases
US4987231A (en) * 1989-08-04 1991-01-22 Pfizer Inc. Optical resolution method for 3R-(3-carboxybenzyl)-6-(5-fluoro-2-benzothiazolyl)methoxy-4R-chromanol
US5155130A (en) * 1989-08-11 1992-10-13 Ciba-Geigy Corporation Certain benzopyran and benzothiopyran derivatives
CA2023492A1 (en) * 1989-08-31 1991-03-01 Barry Clifford Lange Herbicidal glutarimides
WO1991012253A1 (en) * 1990-02-07 1991-08-22 Pfizer Inc. Tetralin and chroman derivatives useful in the treatment of asthma, arthritis, and related diseases
US5073562A (en) * 1990-05-10 1991-12-17 G. D. Searle & Co. Alkoxy-substituted dihydrobenzopyran-2-carboxylic acids and derivatives thereof
US5082847A (en) * 1990-07-18 1992-01-21 Syntex (U.S.A.) Inc. Carbostyril compounds connected via an oxyalkyl group with a piperidine ring and having pharmaceutical utility
US5120758A (en) * 1991-02-08 1992-06-09 Ciba-Geigy Corporation Certain benzodioxole, benzodioxane and benzodioxepin derivatives useful as 5-lipoxygenase inhibitors
US5149703A (en) * 1991-09-06 1992-09-22 Merck Frosst Canada, Inc. Quinoline-substituted chromans and related compounds as leukotriene antagonists
MX9300312A (en) * 1992-01-23 1993-07-31 Pfizer BENZOPYRANIC AND RELATED ANTAGONISTS OF LTB.
US5698550A (en) * 1993-06-14 1997-12-16 Pfizer Inc. Tetralin and chroman derivatives useful in the treatment of asthma, arthritis and related diseases
IL112568A0 (en) * 1994-02-10 1995-05-26 Wyeth John & Brother Ltd Substituted quinolines, their preparation and pharmaceutical compositions containing them
JPH10273467A (en) * 1997-01-29 1998-10-13 Snow Brand Milk Prod Co Ltd New tetralone or benzopyranone derivative and its production
ZA981080B (en) * 1997-02-11 1998-08-12 Warner Lambert Co Bicyclic inhibitors of protein farnesyl transferase
US6162810A (en) * 1997-11-17 2000-12-19 The Regents Of The University Of California Inadone and tetralone compounds for inhibiting cell proliferation
US5952326A (en) * 1997-12-10 1999-09-14 Pfizer Inc. Tetralin and chroman derivatives useful in the treatment of asthma, arthritis and related diseases
BR0309278A (en) * 2002-04-17 2005-04-26 Cytokinetics Inc Compound, composition, methods for modulating ksp kinesin activity, for inhibiting ksp, for the treatment of a cell proliferative disease, and use of a compound
ATE532777T1 (en) 2004-09-21 2011-11-15 Marshall Edwards Inc SUBSTITUTED CHROMEDER DERIVATIVES, MEDICATIONS AND APPLICATIONS IN THERAPY
US8080675B2 (en) 2004-09-21 2011-12-20 Marshall Edwards, Inc. Chroman derivatives, medicaments and use in therapy
JP2008531558A (en) * 2005-02-25 2008-08-14 イーライ リリー アンド カンパニー Novel lipooxygenase inhibitors
WO2012061409A1 (en) 2010-11-01 2012-05-10 Marshall Edwards, Inc. Isoflavonoid compounds and methods for the treatment of cancer
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