AP779A

AP779A - Apolipoprotein-B synthesis inhibitors.

Info

Publication number: AP779A
Application number: APAP/P/1997/000968A
Authority: AP
Inventors: Jan Heeres; Leo Jacobus Josef Backx; Robert Josef Maria Hendrickx; Der Eycken Luc Alfons Leo Van; Courcelles Didier Robert Guy Gabriel Der Chaffoy De
Original assignee: Janssen Pharmaceutica Nv
Priority date: 1994-10-27
Filing date: 1995-10-19
Publication date: 1999-11-03
Also published as: NO971895D0; IL115771A; CA2203274C; AU3868095A; CZ119897A3; EP0788496B1; DE69519995D1; OA10479A; BG101402A; HRP950532B1; CY2256B1; FI119548B; IL115771A0; DE122007000005I1; SK50797A3; AP9700968A0; BG63694B1; US5929075A; ES2155535T3; RO118715B1

Abstract

The present invention provides novel compounds of formula (I), the the stereochemically iso-forms thereof, and the phar-maceutically acceptable acid addition salts thereof, wherein A and B taken together form a bivalent radical of formula: -N=CH- (a), -CH=N- (b), -CH2-CH2- (c), -CH-CH- (d), -C(-O)- CH2- (e), -CH2-C(-O)- (f); R1 is hydrogen, Ci-6alkyl, or halo; R2 is hydrogen or halo; R3 is hydrogen; Ci-salkyi; Ca-ecycloalkyl or Ci-g alkyl substituted with hydroxy, oxo, C3-6cycloalkyl or aryl. Het is five- or six membered optionally substituted heterocyclic ring. The use as a medicine, especially as a lipid lowering agent is disclosed as well as pharmaceutical compositions and processes for preparing compounds and compositions.

Description

APOLIPOPROTEIN-B SYNTHESIS INHIBITORS

The present invention concerns novel compounds of formula (I), pharmaceutical 5 compositions comprising said compounds, the preparation thereof as well as the use as a medicine in the treatment of hyperlipidemia.

The causal relationship between hypercholesterolemia, particularly that associated with increased plasma concentrations of low density lipoproteins (LDL) and veiy low density lipoprotein (VLDL) remnants, and premature atherosclerosis has gained widespread acceptance over the last few years. The consensus that treatment of hypercholesterolemia has therapeutic benefit has become widely accepted by both physicians and the public.

A limited number of drugs are available for the treatment of hyperlipidemia. The primary agents used for die management of hyperlipidemia included bile acid sequestrants, fibrates, nicotinic acid and HMG Co A-reductase inhibitors. The inconvenience of administration and gastro-intestinal side-effects of available bile acid sequestrants make compliance a major problem. The fibrates have only limited usefulness in the treatment for certain types of hypercholesterolemia. Treatment with nicotinic acid encompasses side-effects and toxicity problems. The HMG Co A20 reductase inhibitors already form a first line treatment of familiar hypercholesterolemia. However there still remains a need for new lipid lowering agents for that act preferably via other mechanisms than the above mentioned drugs.

EP-0,006,711-A, published on September 9,1980, discloses heterocyclic derivatives of (4-phenylpiperazin-1 -yl-aryloxymethyl-1,3-dioxolan-2-yl)-methyl- l//-imidazoles and

IH-1,2,4-triazoles having antifungal properties. The presently claimed compounds differ therefrom by the presence of a sulfur atom adjacent to the Het-moiety and by their pharmacological profile, in particular their apolipoprotein B synthesis inhibiting activity. The present invention provides novel compounds of formula

AP/P/ 97 / 0 0 9 68

.-¾

Al

AP.00779

7.

the A-oxides, the stereochemically isomeric forms thereof, and the pharmaceutically acceptable acid addition salts, wherein A and B taken together form a bivalent radical of formula :

-N=CH- (a),

-CH=N- (b),

-CH2-CH2- (c),

-CH=CH- (d),

-C(=O)-CH2- (e),

-CH₂-C(=O)- (f), in the bivalent radicals of formula (a) and (b) the hydrogen atom may be replaced by Ci-6alkyl; in the bivalent radicals of formula (c), (d), (e), (f), one or two hydrogen atoms may be replaced by Ci-galkyl;

R¹ is hydrogen, Cj^alkyl or halo;

R² is hydrogen or halo;

R³ is hydrogen; Ci-8alkyl: C3-6Cycloalkyl; or Ci-salkyl substituted with hydroxy, oxo, C3-6cycloalkyl or aryl;

Het is a heterocycle selected from the group consisting of pyridine; pyridine substituted with one or two substituents selected from Ci^alkyl, hydroxy, Ci-6alkyloxy, trihalomethyl, amino, mono- or di(Ci-6alkyl)amino or aryl; pyrimidine; pyrimidine substituted with one or two substituents selected from Ci^alkyl, hydroxy, Ci-6alkyloxy, trihalomethyl, amino, mono- or di(Ci-6alkyl)-amino or aryl; tetrazole; tetrazole substituted with Cusalkyl or aryl; triazole; triazole substituted with one or two substituents selected from Ci^alkyl, hydroxy, Ci_6alkyloxy, trihalomethyl, amino, mono- or di(Ci^alkyl)-amino; thiadiazole; thiadiazole substituted with one or two substituents selected from Ci^alkyl, hydroxy, Ci-6alkyloxy, trihalomethyl, amino, mono- or di(Ci-6alkyl)amino; oxadiazole substituted with one or two substituents selected from Cj^alkyl, hydroxy, Cj-ealkyloxy, trihalomethyl, amino, mono- or di(Ci-6alkyl)amino; imidazole; imidazole substituted with one or two substituents selected from Cj^aikyl, hydroxy, Ci-6alkyloxy, trihalomethyl, amino, mono- or di(Ci^alkyl)amino; thiazole; thiazole substituted with one or two substituents selected from Cj^alkyl, hydroxy, Ci-ealkyloxy, trihalomethyl, amino, mono- or di(Ci^alkyl)amino; oxazole; oxazole substituted with one or two substituents selected from Ci-^alkyl, hydroxy, Cj-ealkyloxy, trihalomethyl, amino, mono- or di(Ci^alkyl)amino;

aryl is phenyl or phenyl substituted with Cj.ealkyl or halo.

AP/P/ 97/00968

The heterocyclic radical “Het” is bound to the sulfur atom via a carbon atom.

AP.00779

-3As used in the foregoing definitions halo is generic to fluoro, chloro, bromo and iodo; Cj-galkyl defines straight and branched chain saturated hydrocarbon radicals having from 1 to 6 carbon atoms such as, for example, methyl, ethyl, propyl, butyl, pentyl, hexyl, 1-methylethyl, 2-methylpropyl and the like; Cj.galkyl defines Cj^alkyl and the higher homologues thereof containing 7 or 8 carbon atoms such as, for example, heptyl or octyl and the branched isomers thereof. C3_6cycloalkyl defines saturated cyclic hydrocarbon radicals having from 3 to 6 carbon atoms, such us cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl.

Het may in particular be a radical of formula

S

AP/P/ 9 7 / 0 0 9 68

’-•Jr wherein :

R⁴ is hydrogen or Cj^alkyl;

R⁵ and are hydrogen, Ci-6alkyl or amino;

R⁷ is hydrogen or Cj^alkyl;

each R⁸ independently is hydrogen or Cj^alkyl;

each R⁹ independently is hydrogen, Ci-6alkyl, trifluoromethyi, amino or hydroxy;

R¹⁰ and R¹¹ each independently are hydrogen or Ci-6alkyl;

R13 is hydrogen or Cj^alkyl;

r14 is hydrogen, Cj-galkyl or hydroxy;

R¹⁵ is hydrogen or Cj-galkyl.

AP . Ο Ο 719

-4The pharmaceutically acceptable acid addition salts as mentioned hereinabove are meant to comprise the therapeutically active non-toxic acid addition salt forms which the compounds of formula (I) are able to form. The latter can conveniently be obtained by treating the base form with such appropriate acid. Appropriate acids comprise, for example, inorganic acids such as hydrohalic acids, e.g. hydrochloric or hydrobromic acid; sulfuric ; nitric ; phosphoric and the like acids; or organic acids such as, for example, acetic, propanoic, hydroxyacetic, lactic, pyruvic, oxalic, malonic, succinic, maleic, fumaric, malic, tartaric, citric, methane-sulfonic, ethanesulfonic, benzenesulfonic, p-toluenesulfonic, cyclamic, salicylic, p-aminosalicylic, pamoic and the like acids. The term addition salt as used hereinabove also comprises the solvates which the compounds of formula (I) as well as the salts thereof, are able to form. Such solvates are for example hydrates, alcoholates and the like. Conversely the salt form can be converted by treatment with alkali into the free base form.

The term “stereochemically isomeric forms” as used hereinbefore defines all the possible isomeric forms which the compounds of formula (I) may possess. Unless otherwise mentioned or indicated, the chemical designation of compounds denotes the mixture of all possible stereochemically isomeric forms, said mixtures containing all diastereomers and enantiomers of the basic molecular structure. More in particular, stereogenic centers may have the R- or S-configuration; substituents on bivalent cyclic saturated radicals may have either the cis- or rranj-configuration. Stereochemically isomeric forms of the compounds of formula (I) are obviously intended to be embraced within the scope of this invention.

The A-oxide forms of the compounds of formula (I) are meant to comprise those compounds of formula (I) wherein one or several nitrogen atoms are oxidized to the so-called A-oxide, particularly those A-oxides wherein one or more of the pipp.ra7.inpnitrogens are A-oxidized.

The substituents on the dioxolane moiety of the compounds of formula (I) may have the cis- or trans-configuration. The compounds of formula (I) having the cis-configuration are preferred.

The compounds of formula (I) wherein the stereogenic carbon in the 2-position of the dioxolane moiety has the S-configuration are also preferred.

The compounds of formula (I) may also exist in their tautomeric forms. For instance, heterocycies such as, for example, pyridine, pyrimidine, triazole, thiadiazole, oxadiazole, imidazole, thiazole and oxazole, which are substituted with hydroxy, amino

AP/P/ 97 / 0 0 9 68

AP. Ο Ο 7 7 ί

-5or Ci-6alkylamino may exist in their tautomeric form. Such forms although not explicitly indicated in the above formula are intended to be included within the scope of the present invention.

A group of interesting compounds are those compounds of formula (I) wherein Rl is chloro or fluoro, especially chloro.

Also a group of interesting compounds are those compounds of formula (I) wherein R¹is Ci-6alkyl, especially methyl.

A further group of interesting compounds are those compounds of formula (I) wherein R² is hydrogen, chloro or fluoro, preferably hydrogen.

Another group of interesting compounds of formula (I) are those compounds wherein 15 the bivalent radical -A-B- is -CH=CH-, -N=CH- or -CH=N-, especially -CH=N- or

-N=CH-. In said bivalent radicals, the hydrogen atom may be replaced by Cj-galkyl, especially methyl.

A particular group of compounds are those compounds of formula (I) and especially 20 those interesting compounds wherein R² is Ci-8-alkyl or Cs-g-cycloalkyl, preferably butyl, pentyl or cyclopentyl.

A group of preferred compounds of formula (I) is formed by those compounds wherein Het is a triazole, substituted triazole, imidazole, substituted imidazole, thiazole, substituted thiazole.

More preferred compounds of formula (I) are those interesting or particular compounds wherein Het is 2-thiazolyl, 4-methyl-4//-1,2,4-triazol-3-yl, 4//-1,2,4-triazol-3-yl,

2-methyl-2//-l,2,4-triazol-3-yl or 2//-1,2,4-triazol-3-yl.

Most preferred compounds are cis-4-[4-[4-[4-[[2-(4-chlorophenyl)-2-[[(4-methyl-4//-l,2,4-triazol-3-yl)thio]methyl]1.3- dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-2,4-dihydro-2-(l-n^thylpropyl)-3//-l,2,4-triazol-3-one;

cis-2- [4-[4- [4-[ [2- (4-chlorophenyl)-2-[ [(4-methyl-4//-1,2,4-triazol-3-yl) thio]methyl]1.3- dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-2,4-dihydro-4-(l-methylpropyI)-3//-l,2,4-triazol-3-one;

cis'-2-[4-[4-[4-[[2-(4-fluorophenyl)-2-[[(4-methyl-4//-l,2,4-triazol-3-yl)thio]methyl]1.3- dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-4-cyclopentyl-2,4-dihydro40 3//-1,2,4-triazol-3-one;

89600/Z6 /d/dV

AP.00779

-6cri-2-[4-[4-[4-[[2-(4-chlorophenyl)-2-[[(4-methyl-4//-l,2,4-mazol-3-yl)thio]methyl]l,3-dioxolan-4-yl]methoxy]phenyI]-l-piperazinyl]phenyl]-2,4-dihydro-4-pentyl-3//l,2,4-triazol-3-one;

cz5-4-(l-ethylpropyl)-2-[4-[4-[4-[[2-(4-fluorophenyl)-2-[[(4-methyl-4//-l,2,4-triazol-35 yl)thio]methyl]-l,3-dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-2,4-dihydro3//-1,2,4-triazol-3-one; a pharmaceutically acceptable acid addition salt or a stereochemically isomeric form thereof.

The compounds of formula (I) may be prepared by (9-alkylating a phenol of formula (Π) 10 with a 1,3-dioxolane derivative of formula (ΙΠ), wherein W represents an appropriate leaving group such as halo, e.g. chloro or bromo, or a sulfonyloxy leaving group, e.g. 4-methylbenzenesulfonyloxy (tosylate) or methanesulfonyloxy (mesylate).

Said O-alkylation reaction can conveniently be conducted following art-known procedures, e.g. by stirring and heating the reactants in an appropriate solvent such as a dipolar aprotic solvent, e.g. N, //-dimethylformamide, ////-dime thy lace tarn ide, in the presence of a base such as, an alkali metal hydroxide or carbonate, e.g. sodium or potassium hydroxide, or sodium or potassium carbonate.

APiP! 97/00968

Intermediates of formula (H) may be prepared in similar ways as disclosed in EP-0,006,711, mentioned hereinabove. EP-0,331,232-A, published on September 6, 1989 and WO 93/19061, published on September 30, 1993, also disclose ways of preparing intermediates of formula (Π).

The compounds of formula (I) may also be prepared by reacting an intermediate of formula (TV), wherein W is an appropriate leaving group as defined hereinabove with a heterocyclic derivative of formula (V).

AP.00779

W/'

Said reaction may be performed by stining an heating the intermediates in an appropriate solvent such as a dipolar aprotic solvent, e.g. N, A-dimethylformamide, MV-dimethyl5 acetamide , dimethylsulfoxide, in the presence of a base such as, an alkali metal carbonate or hydroxide, e.g. sodium or potassium carbonate, or sodium or potassium hydroxide.

Compounds of formula (I) may also be converted into each other.

For instance, the compounds wherein R³ is Ci-galkyl substituted with hydroxy may be prepared by reduction of the corresponding compounds of formula (Ϊ) wherein R³ is Cj-galkyl substituted with oxo. The compounds of formula (I) wherein an endocyclic or exocyclic nitrogen atom of the heterocyclic radical 'Het is substituted with a Cj-galkyl may be prepared from the corresponding compounds wherein said endocyclic or exocyclic nitrogen atom is unsubstituted by art-known TV-alkylation reactions.

The compounds of formula (I) wherein R³ is other than hydrogen may be prepared from compounds of formula (I) wherein R³ is hydrogen by art-known /V-alkylation reactions.

The compounds of formula (I) may also be converted to the corresponding A-oxide forms following art-known procedures for converting a trivalent nitrogen into its

A'-oxide form. Said A-oxidation reaction may generally be carried out by reacting the starting material of formula (I) with an appropriate organic or inorganic peroxide. Appropriate inorganic peroxides comprise, for example, hydrogen peroxide, alkali metal or earth alkaline metal peroxides, e.g. sodium peroxide, potassium peroxide; appropriate organic peroxides may comprise peroxy acids such as, for example, benzenecarboperoxoic acid or halo substituted benzenecarboperoxoic acid, e.g. 3-chlorobenzenecarboperoxoic acid, peroxoalkanoic acids, e.g. peroxoacetic acid, alkylhydroperoxides, e.g. t.butyl hydroperoxide. Suitable solvents are, for example, water, lower alkanols, e.g. ethanol and the like, hydrocarbons, e.g. toluene, ketones, e.g. 2-butanone, halogenated hydrocarbons, e.g. dichloromethane, and mixtures of such solvents.

AP/P/ 9 7 / 0 0 9 68

Intermediates of formula (HI), which are deemed novel, may be prepared by the following rectionsequence. A heterocyclic reagent (V) is S-alkylated with an intermediate of formula (VI), wherein W is an appropriate leaving group as defined

AP. Ο Ο 7 7 9

-8hereinabove, by stirring and heating the intermediates in an appropriate reaction-inert solvent such as a ketone, e.g. acetone, in the presence of a base such as a alkali metal carbonate or hydoxide, e.g. sodium or potassium carbonate, sodium or potassium hydroxide. The thus formed ketone of formula (VH) is then converted into the corresponding ketal of formula (VUI) by stirring and heating the intermediate of formula (VH) with glycerol in ±e presence of an acid such as for example p-toluenesulfonic acid in a reaction inert solvent such as toluene. Finally, the hydroxylfunction of the intermediate of formula (VUI) is converted into an appropriate leaving group by artknown functional group transformation reations, such as, for example, converting the hydroxyl group into a tosylate by reaction with p-toluenesulfonylchloride .

The intermediates of formula (IV) may be prepared be prepared in an analogous way.

AP/P/ 97 / 0 0 9 68

An intermediate of formula (VI) is ketalized as described hereinabove. Subsequently, the hydroxyl function is converted into an appropriate leaving group, e.g. a sulfonyloxy group. Reaction of the thus formed intermediate (IX) with an intermediate (Π) results in an intermediate (TV).

Pure stereochemically isomeric forms of the compounds of formula (I) may be obtained by the application of art-known procedures. Diastereomers may be separated by physical separation methods such as selective crystallization and chromatographic techniques, e.g. liquid chromatography. Enantiomers may be separated from each other

AP .00779

-9by forming diastereomeric salt forms with optically pure chiral acids and subsequent selective crystallization. Said pure stereochemically isomeric forms may also be prepared from the corresponding stereochemically isomeric forms of the appropriate starting materials, provided that the reaction occurs stereospecifically. Preferably if a specific stereochemically isomeric form is desired, said form will be synthesized by stereospecific methods of preparation. These methods will advantageously employ enantiomerically pure starting materials.

The present compounds inhibit the synthesis of apolipoprotein B as can be evidenced by 10 the results obtained in the Apolipoprotein B (apo B) inhibition test as described hereinafter. Apolipoprotein B is the principal protein component of veiy low density lipoproteins (VLDL) and low density lipoproteins (LDL). Approximately 60 to 70% of the total serum cholesterol is transported in LDL. Increased concentration of LDLcholesterol in serum is causally related to atherosclerosis. By inhibiting the synthesis of apolipoprotein B the amount of noxious low density lipoproteins is decreased.

The present compounds show no or little undesired side-effects such as, for example, albumine inhibiting activity, androgen biosynthesis inhibiting activity or cholesterol biosynthesis inhibiting activity.

In view of their apolipoprotein B inhibiting activity and concommitant lipid lowering activity the present compounds are useful as a medicine especially in a method of treating patients suffering from hyperlipidemia. In particular the present compounds may be used for the manufacture of a medicine for treating disorders caused by an excess of very low density lipoproteins (VLDL) or low density lipoproteins (LDL), and especially disorders caused by the cholesterol associated with said VLDL and LDL.

A large number of genetic and acquired diseases can result in hyperlipidemia. They can be classified into primary and secondary hyperlipidemic states. The most common causes of the secondary hyperlipidemias are diabetes mellitus, alcohol abuse, drugs, hypothyroidism, chronic renal failure, nephrotic syndrome, cholestasis and bulimia. Primary hyperlipidemias are common hypercholesterolaemia, familial combined hyperiipidaemia, familial hypercholesterolaemia, remnant hyperiipidaemia, chylomicronaemai syndrome, familial hypertriglyceridaemia. The present compounds may also be used to prevent or treat patients suffering from atherosclerosis, especially coronary atherosclerosis and more in general disorders which are related to atherosclerosis, such as ischaemic heart disease, peripheral vascular disease, cerebral vascular disease. The present compounds may cause regression of atherosclerosis and inhibit the clinical consequences of atherosclerosis, particularly morbidity and mortality.

AP/P/ 97 / 0 0968

AP. Ο Ο 7 7 9

-10Ιη view of their apolipoproteine B inhibiting activity the subject compounds may be formulated into various pharmaceutical forms for administration purposes. To prepare these pharmaceutical compositions, an effective amount of a particular compound, in base or acid addition salt form, as the active ingredient is intimately mixed with a pharmaceutically acceptable carrier. Said carrier may take a wide variety of forms depending on the form of preparation desired for administration. These pharmaceutical compositions are desirably in unitary dosage form suitable, preferably, for administration orally, rectally or by parenteral injection. For example, in preparing the compositions in oral dosage form, any of the usual pharmaceutical media may be employed, such as, for example, water, glycols, oils, alcohols and the like in the case of oral liquid preparations such as suspensions, syrups, elixirs and solutions; or solid if? carriers such as starches, sugars, kaolin, lubricants, binders, disintegrating agents and the like in the case of powders, pills, capsules and tablets. Because of their ease in administration, tablets and capsules represent the most advantageous oral dosage unit form, in which case solid pharmaceutical carriers are obviously employed. For parenteral compositions, the carrier will usually comprise sterile water, at least in large part, though other ingredients, for example, to aid solubility, may be included. Injectable solutions, for example, may be prepared in which the carrier comprises saline solution, glucose solution or a mixture of saline and glucose solution. Injectable suspensions may also be prepared in which case appropriate liquid carriers, suspending agents and the like may be employed. In the compositions suitable for percutaneous administration, the carrier optionally comprises a penetration enhancing agent and/or a suitable wetting ‘λ .. 5* agent, optionally combined with suitable additives of any nature in minor proportions, which additives do not cause a significant deleterious effect to the skin. Said additives may facilitate the administration to the skin and/or may be helpful for preparing the desired compositions. These compositions may be administered in various ways, e.g., as a transdermal patch, as a spot-on, as an ointment. Acid addition salts of the compounds of formula (I) due to their increased water solubility over the corresponding base form, are obviously more suitable in the preparation of aqueous compositions.

It is especially advantageous to formulate the aforementioned pharmaceutical compositions in dosage unit form for ease of administration and uniformity of dosage. Dosage unit form as used in the specification herein refers to physically discrete units suitable as unitary dosages, each unit containing a predetermined quantity of active ingredient calculated to produce the desired therapeutic effect in association with the required pharmaceutical carrier. Examples of such dosage unit forms are tablets (including scored or coated tablets), capsules, pills, powder packets, wafers, injectable

AP/P/ 9 7 / 0 0 968

AP . Ο Ο 7 7 9

-11solutions or suspensions, teaspoonfuls, tablespoonfuls and the like, and segregated multiples thereof.

Those of skill in the treatment of hyperlipidemia could easily determine the effective 5 daily amount from the test results presented hereinafter. In general it is contemplated that a therapeutically effective dose would be from 0.001 mg/kg to 5 mg/kg body weight, more preferably from 0.01 mg/kg to 0.5 mg/kg body weight, it may be appropriate to administer the therapeutically effective dose as two, three, four or more sub-doses at appropriate intervals throughout the day. Said sub-doses may be formulated as unit dosage forms, for example, containing 0.05 mg to 250 mg, and in particular 0.5 to 5 mg of active ingredient per unit dosage form.

$

The exact dosage and frequency of administration depends on the particular compound of formula (I) used, the particular condition being treated, the severity of the condition being treated, the age, weight and general physical condition of the particular patient as well as other other medication the patient may be taking, as is well known to those skilled in the art. Furthermore, it is evident that said effective daily amount may be lowered or increased depending on the response of the treated patient and/or depending on the evaluation of the physician prescribing the compounds of the instant invention.

The effective daily amount ranges mentioned hereinabove are therefore only guidelines.

Experimental part

Hereinafter, the term DIPE means diisopropylether, MIK means me±ylisopropyl ketone and DMF means ^//-dimethylformamide.

& Si

A. Preparation of the intermediates Example 1

a) A mixture of 1-methy 1-1//-1,2,4-triazole-5-thioI (35 g), 2-chloro-l-(fluorophenyl)ethanone (51.4 g) and sodium carbonate (32.5 g) in 2-propanone (500 ml) was stirred and refluxed for 4 hours. The solvent was evaporated, the residue was dissolved in CH2CI2, filtered and the filtrate evaporated. The residue was crystallized from DIPE, yielding 25 g (33%) of product. A sample (3 g) was purified by column chromatography over silica gel (eluent: CH2CI2/CH3OH 99/1). The pure fractions were collected and evaporated. The residue was crystallized from DIPE, yielding 1-(435 fluorophenyl)-2-[(2-methyl-2//-1,2,4-triazol-3-yl)thio]ethanone (interm. 1).

b) A mixture of intermediate (1) (22 g), glycerol (39.6 g) and /7-toluenesulfonic acid (20 g) in toluene (200 ml) was stirred and refluxed overnight. The mixture was cooled and water was added. The mixture was extracted with toluene and washed with water.

AP/P/ 9 7 / 0 0 9 68

AP . Ο Ο 7 7 9

-12The organic layer was dried, filtered and the solvent evaporated. The residue was purified by HPLC over silica gel (eluent: CH2CI2/CH3OH 98/2). The pure fractions were collected and evaporated, yielding 9 g (31.6%) of (±)-cz's-2-(4-fluorophenyl)-2[[(2-methyl-2//-l,2,4-triazol-3-yl)thio]methyl]-l,3-dioxolane-4-methanol (interm. 2).

c) A mixture of intermediate (2) (9 g), p-toluene sulfonyl chloride (6.3 g) and

A/V-dimethyl-4-pyridinamine (1 g) in CH2CI2 (150 ml) and AQV-diethylethanamine (5 ml) was stirred at room temperature for 4 hours. Water was added and the layers were separated. The organic layer was washed with water, dried, filtered and the solvent evaporated. The residue was purified by column chromatography over silica gel (eluent: CH2CI2/CH3OH 99/1). The pure fractions were collected and evaporated at a temperature < 35°C. The residue was dissolved in MIK and converted into the p-toluenesulfonic acid salt (1:1). A little DOPE was added and the product was crystallized out. The precipitate was filtered off and dried, yielding 6.8 g (37.8%) of (±)-czs-2-(4-fluorophenyl)-2-[[(2-methyl-2H-l,2,4-triazol-3-yl)thio]methyl]-l,315 dioxolane-4-methanol 4-methylbenzenesulfonate 4-methylbenzenesulfonate(l:l) (interm. 3).

In a similar matter were also prepared :

(±)-czj-2-(4-fluorophenyl)-2-[[(4-methyl-4/7-l,2,4-triazol-3-yl)thio]methyl]-l,3dioxolan-4-methanol 4-methylbenzenesulfonate(ester) 4-methylbenzenesulfonate(l: 1);

mp. 136.4°C (interm. 4);

(±)-cz's-2-(2,4-difluorophenyl)-2-[[(4-methyl-4//-l,2,4-triazol-3-yl)thio]methyl]-l,3dioxolane-4-methanol 4-methylbenzenesulfonate(ester) 4-methylbenzenesulfonate (1:1) (interm. 5);

(±)-fra/u-2-(4-chlorophenyl)-2-[[(4-methyl-4//-l,2,4-triazol-3-yl)thio]methyl]-l,325 dioxolane-4-methanol 4-methylbenzenesulfonate(ester) 4-methylbenzenesulfonate (1:1); mp. 151.9°C (interm. 6);

(±)-cz's-2-(2,4-difluorophenyl)-2-[[(2-methyl-2H-l ,2,4-triazol-3-yl)thio)methyl]-1,3dioxolane-4-methanol 4-methylbenzenesulfonate (ester) (interm. 7); and (±)-cz's-[2-(bromomethyl)-2-(2,4-difluorophenyl)-l,3-dioxolan-4-yl]methyl

2-naphthalenesulfonate (interm. 40).

Example 2

a) A mixture of 2-bromo-l-(4-chlorophenyl)etnanone (350 g), glycerine (322 g) and p-toluenesulfonic acid (35 g) in toluene (3000 ml) was stinted and refluxed for 24 hours, using a water separator. The reaction mixture was poured into an aqueous

NaHCC>3 solution and stirred for a while. The organic layer was separated, dried,

AP/P/ 97 / 0 0 9 68

AP. Ο Ο 7 7 9

-13filtered and the solvent was evaporated, yielding 485 g (93%; oil) of (cis +trans)-2(bromomethyl)-2-(4-chlorophenyl)-1,3-dioxolane-4-methanol (interm. 8a). 2-Napthalenesulfonyl chloride (21 g) was added porbonwise to a mixture of intermediate (8a) (25 g) and AUV-dimethyl-4-pyridinamine (1 g) in AJV-diethylethanamine (25 ml) and CH2CI2 (250 ml) and the mixture was stirred at room temperature for hours. The mixture was poured into water and washed. The organic layer was dried, filtered and the solvent evaporated. The residue was purified by column chromatography over silica gel (eluent: CH2CI2/CH3OH 99/1). The pure fractions were collected and evaporated. The residue was purified by column chromatography (eluent: CH2CI2/ hexane 40/60 to 60/40). The pure fractions were collected and evaporated, yielding 21.8 g (55%) of (±)-cis-[2-(bromomethyl)-2-(4-chlorophenyI)'•j? l,3-dioxolan-4-yl]methyl 2-naphthalenesulfonate (interm. 8b).

c) 2,4-dihydro-4-[4-[4-(4-hydroxyphenyl)-l-piperazinyl]phenyl]-2-(l-methylpropyl)3//-1,2,4-triazoI-3-one (206.9 g) was added to a solution of intermediate (8b) (250 g) in dimethyl sulfoxide (2000 ml). Potassium hydroxide (67 g) was added and the reaction mixture was stirred overnight at room temperature. The mixture was poured into water (3000 ml) and stirred for 30 minutes. The precipitate was filtered off, washed with 2-propanol (1000 ml) and DIPE (1000 ml), then dried, yielding 316 g (92.2%) of (±)-cii-4-[4-[4-[4-[[2-(bromomethyl)-2-(4-chlorophenyl)-l,3-dioxolan-4-yl]methoxy]20 phenyl]-1 -piperazinyl]phenyl] -2,4-dihydro-2-( 1 -methylpropy 1)-3//-1,2,4-triazol- 3-one (interm. 8c).

AP/P/ 9 7 / 0 0 9 68

In a similar manner were prepared: Table 1

Int. No.	R¹	R²	A-B	R³	Physical data
8c	Cl	H	CH=N	CH(CH₃)CH2CH3	cis
9	Cl	H	N=CH	CH(CH₃)2	mp. 185.8°C; cis
10	Cl	H	CH=N	CH₂CH(CH3)2	mp. 168.3°C; cis
11	Cl	H	N=CH	CH₂CH(CH₃)2	mp. 175.6°C; cis
12	Cl	H	N=CH	CH(CH₃)CH₂CH3	mp. 172.6°C; cis

AP . Ο ϋ 7 7 9

Int. No.	R¹	R2	A-B	R³	Physical data
13	Cl	H	N=CH	CH(CH₂CH₃)2	mp. 164.3°C; cis
14	Cl	H	N=CH	(CH₂)₂CH₃	mp. 201.9°C; cis
15	Cl	H	N=CH	(CH₂)₃CH₃	mp. 153.8°C; cis
16	Cl	H	CH=N	cycloC5H9	cis
17	Cl	H	CH=N	(CH₂)₃CH₃	mp. 172.0°C; cis
18	Cl	H	N=CH	ch₂ch₃	mp. 186.3°C; cis
19	Cl	H	N=CH	(Ch₂)₄ch₃	mp. 164.7°C; cis
20	Cl	H	CH=N	(CH₂)₂CH₃	mp. 172.9°C; cis
21	CI	H	CH=N	ch₂ch₃	mp. 186.6°C; cis
22	Cl	H	N=CH	ch₃	mp. 2O3.9°C; cis
23	Cl	H	CH=N	ch₃	cis
24	Cl	H	CH=N	CH(CH₃)CH₂CH₃	[2S-[2a,4a(R*)]]
25	Cl	H	CH=N	CH(CH₃)CH₂CH₃	[2R-[2a,4a(S*)]]
26	Cl	H	CH=N	CH(CH₃)CH₂CH₃	[2S-[2a,4a(S*)]]
27	Cl	H	CH=N	CH(CH₃)CH₂CH₃	[2R-[2a,4a(R*)]]
28	F	H	CH=N	(CH₂)₂CH(CH₃)₂	mp. 170.3°C; cis
29	F	H	CH=N	CH(CH₂CH₃)₂	cis
30	F	H	CH=N	CH(CH₃)CH₂CH₃	mp. 152.9°C; cis
31	F	H	N=CH	CH(CH₃)CH₂CH₃	mp. 174.2°C; cis
32	F	F	CH=N	CH(CH₂CH₃)₂	cis
33	Cl	H	C(CH₃)₂CO	CH(CH₃)CH₂CH₃	cis
34	Cl	H	COC(CH₃)₂	CH(CH₃)CH₂CH₃	cis
35	Cl	H	C(CH₃)=N	CH(CH₃)CH₂CH₃	cis
36	F	H	CH=N	cycloCsH9	cis
37	F	H	N=CH	cycloC5H9	cis
38	F	H	N=CH	CH(CH₂CH₃)₂	cis
39	F	F	CH=N	cycioCsHg	cis
40	F	H	CH=CH	CH(CH₃)CH₂CH₃	cis
41	Cl	H	CH=CH	CH(C₂H₅)CH₂CH₃	mp. 169.8°C; cis
42	Cl	H	CH=CH	cycloC5H9	mp. 192.7°C; cis
43	F	H	N=CH	(CH₂)₄CH₃	cis
44	Cl	H	N=CH	cycloC5H9	mp. 192.3°C; 2S-cis
45	Cl	H	N=CH	(CH₂)₄CH₃	2S-cis

AP/P/ 9 7 / 0 0 9 68

AP . ο ύ 7 7 9

-15Β. Preparation of the final compounds

Example 3

A mixture of 4-methyl-4/7-l,2,4-triazole-3-thiol (1.9 g), intermediate (8c) (9 g) and sodium carbonate (3 g) in DMF (150 ml) was stirred under N2 at 120°C overnight. The mixture was cooled, diluted with water and the product was crystallized out. The precipitate was filtered off and purified by column chromatography over silica gel (eluent: CH2Cl2/n-hexane/EtOAc/CH3OH 500/250/250/2). The pure fractions were collected and evaporated. The residue was triturated in CH3OH and recrystailized from n-C4H9OH, yielding 6.3 g of (±)-c/s-4-[4-[4-[4-[[2-(4-chlorophenyl)-2-[[(4-methyl10 4H-1,2,4-triazol-3-yl)thio]methyl]- l,3-dioxolan-4-yl]methoxy]phenyl]-1 -piperazinyl]phenyl]-2,4-dihydro-2-(l-methylpropyl)-3/7-l,2,4-triazol-3-one (68%); mp. 173°C (compound 22).

Example 4

A mixture of intermediate (3) (3.3 g), 2,4-dihydro-2-[4-[4-(4-hydroxyphenyl)-lpiperazinyl]phenyl]-4-(l-methylpropyl)-37/-l,2,4-triazol-3-one (2 g) and potassium hydroxide (1 g) in DMF (100 ml) was stirred at room temperature under N2 for 6 hours. Intermediate (3) (1 g) was added again and the mixture was stirred for 1 hour. The mixture was poured into water and filtered. The precipitate was purified by column chromatography over silica gel (eluent: CH2CI2/CH3OH 99/1). The pure fractions were collected and evaporated. The residue was crystallized from MIK, yielding 1.6 g of (±)-cfr-2-[4-[4-[4-[[2-(4-fluorophenyl)-2-[[(2-methyl-2/7-l,2,4-triazol-3-yl)thio]methyl]-1,3-dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-2,4-dihydro-4(l-methylpropyl)-3//-l,2,4-triazol-3-one (45.7%); mp. 157.3°C (compound 70).

Example 5

Sodium hydride , 50% , dispersion in mineral oil (0.31 g) was added to a mixture of compound (76) (4.3 g) in DMF (100 ml) and the mixture was stirred at room temperature for 30 minutes. 2-Bromopropane (0.86 g) was added and the mixture was stirred at room temperature for 48 hours. Sodium hydride , 50% , dispersion in mineral oil and 2-bromopropane were added again and the mixture was stirred for 4 hours. The mixture was poured into water, extracted with CH2CI2 and washed with water. The organic layer was dried, filtered and the solvent evaporated. The residue was purified by column chromatography over silica gel (eluent: CH2CI2/CH3OH 99/1). The pure fractions were collected and evaporated. The residue was crystallized from CH3OH.

The residue was purified by HPLC. The pure fractions were collected and evaporated.

Fraction 1 was crystallized from n-C4H9OH, yielding 0.4 g of (±)-cfr-4-[4-[4-[4-[[2(4-chlorophenyl)-2-[[[ 1 -(1-methylethyl)-1/7-1,2,4-triazol-3-yI]thio]methyI]-l ,3AP/P/ 9 7 / 0 0 9 68 'to

AP . Ο Ο 7 7 9

-16dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-2,4-dihydro-2-(l-methylpropyl)3//-1,2,4-triazol-3-one; mp. 128.8°C (compound 112). Fraction 2 was triturated in CH3OH, yielding 1.4 g of (±)-cfr-4-[4-[4-[4-[[2-(4-chlorophenyl)-2-[[[2-(lmethylethyl)-2//-l,2,4-triazol-3-yl]thio]methyl]-l,3-dioxolan-4-yl]methoxy]phenyl]-l5 piperazinyl]henyl]-2,4-dihydro-2-(l-methylpropyl)-3//-l,2,4-triazol-3-one; mp. 141.2°C (compound 82).

Example 6

A solution of sodium borohydride (1 g) in water (20 ml) was added dropwise to a solution of compound (47) (3.6 g) in DMF (100 ml). The reaction mixture was stirred overnight at room temperature. Acetic acid (1 ml) was added. Water (750 ml) was added, resulting in crystallization of the product. The residue was purified by column chromatography over silica gel (eluent: CH2CI2/CH3OH 90/10). The pure fractions were collected and the solvent was evaporated. The residue was triturated in

2-propanol. The precipitate was filtered off and dried, yielding 2.9 g of (±)-cfr-4-[4-[4[4-[[2-(4-chlorophenyl)-2-[[(4-methyl-4//-l,2,4-triazol-3-yl)thio]methyl]-l,3-dioxolan4-yl ] methoxy] phenyl] -1 -piperaziny l]pheny 1] - 2,4-dihydro-2-(2-hydroxy-1 methylpropyl)-3//-l,2,4-triazol-3-one; mp. 153.4°C (compound 48).

Table 2

AP/P/ 97 / 0 0 9 68

Co. No	Ex. No.	R¹	R2	R3	physical data
1	3	Cl	H	CH(CH₃)₂	mp. 194.8°C /cis
2	3	Cl	H	CH(CH₃)CH₂CH₃	mp. 147.8°C/ cis
3	3	Cl	H	CH₂-CH(CH₃)2	mp. 182.5°C/cfr
4	4	F	H	CH(CH₃)2	mp. 181.1°C/cfr
5	4	F	H	CH₂-CH(CH₃)₂	mp. 166.4°C / cis
6	3	Cl	H	cyclo(C5H9)	mp. 198.8°C/cfr
7	3	Cl	H	CH(CH₂CH₃)₂	mp. 139.6°C/cts
8	3	Cl	H	(CH₂)₂CH₃	mp. 184.6°C / cis
9	4	F	H	CH(CH₃)CH₂CH₃	mp. 180.0°C/ cis
10	4	F	F	CH(CH₃)CH₂CH₃	mp. 180.7°C /cis

AP . Ο Ο 7 7 9

Co. No	Ex. No.	R¹	(^R2	R³	physical data
11	4	F	IH	cycloCCsHc))	mp. 194.2°C / cis
12	4	F	1 H	CH(CH₂CH₃)₂	mp. 144.3°C / cis
13	4	F	F	cyclo(C5H9)	mp. 202.4°C / cis
14	4	F	F	CH(CH₂CH₃)₂	mp. 166.7°C 1 cis
15	3	Cl	H	(CH₂)₃CH₃	mp. 194.6°C/ cis
16	3	Cl	H	ch₂-ch₃	mp. 218.3°C / cis
17	3	Cl	H	CH₂-CH(OH)-C(CH₃)₃	mp. 205.9°C / cis
18	3	Cl	H	(CH₂)₄CH₃	mp. 173.8°C / cis
19	4	Cl	H	CH(CH₃)CH₂CH₃	mp. 140.9°C / trans
20	4	Cl	H	ch₃	mp. 208.6°C / cis
21	4	Cl	H	CH(CH₃)CH(OH)(CH₃)	mp. 202.4°C / cis
133	3	ch₃	H	(CH₂)₄CH₃	mp. 147.4°C / cis
134	3	Br	H	(CH₂)₄CH₃	mp. 152.5°C / cis
136	3	Cl	H	cycloiCsHg)	2S-cis
137	3	Cl	H	(CH₂)₄CH₃	2S-cis

Table 3

ΑΡ/Ρ/ 97 / 0 0 9 68

Co. No	Ex. No.	R¹	R2	R³	-X-	physical data
22	3	Cl	H	CH(CH₃)CH₂CH₃	/-s —_ N—	mp. 176.9°C / cis
23	3	Cl	H	CH₂CH(CH₃)₂	—N N —	mp. 192.9°C/ cis
24	3	Cl	H	cyclo(C5H9)	— K N —	mp. 2iQ.2°C / cis
25	4	F	H	CH₂CH(CH₃)₂	—/	mp. 180.6°C/c/j
26	3	Cl	H	(CH₂)₃CH₃	—^N\ /^N~	mp. 194.1 °C / cis

AP. Ο Ο 7 7 9

-18’.2»

Co. No	Ex. No.	R¹	R²
27	3	Cl	H
28	4	F	H
29	4	F	F
30	3	Cl	H
31	3	Cl	H
32	4	Cl	H
33	4	Cl	H
34	4	Cl	H
35	4	F	H
36	4	Cl	H
37	4	F	F
38	3	Cl	H
39	3	Cl	H
40	J	Cl	H
41	0 3	Cl	H
42	3	F	H

R3

-Xphysical data (CH₂)₂CH₃

CH(CH₃)C.H₂CH₃

CH(CH₃)CH₂CH₃

CH2-CH3 ch₃ (ch₂)₄ch₃

CH(CH₃)2

CH₂-CH(OH)-C(CH₃)₃ cyclo(C5H9)

CH(CH₃)CH₂CH₃ cyclo(C5H9)

CH(CH₃)CH₂CH₃

-N N — \__/ — Ν N—

N-N N—

-Ν N-N N-Ν N—

-Ν N— /—\

-Ν NV7

-Ν N —

-Ν N—

CH(CH₃)CH₂CH₃ (CH₂)₂CH(CH₃)₂

-Ν N-N N—

-Ν N\_y —Ν Nmp. 187.3°C/c/j· mp. 157.5°C/ cis mp. 146.4°C/c/s mp. 195.5°C / cis mp. 161.2°C / cis mp. 191.7°C / cis mp. 157.2°C/cis mp. 189.9°C / cis mp. 198.2°C/cw mp. 18O.7°C I trans mp. 185.2°C/cfr mp. 187.0°C / [a]p° = -24.5° (c = 0.5% in DMF) -)-[2S-[2a,4a(R*)]] mp. 155.1°C/[a]p° = +34.64° (c = 0.5% in DMF) (+)-[2R-[2a,4a(S*)]] mp. 156.4°C 20 /[«Id =-33.1° (c = 0.5% in DMF) (-)-[2S-[2a,4a(S*)]] mp. 187.7°C/ [a]2° = +24.65° (c = 0.5% in DMF) (+)-[2R-[2a,4a(R*)]] mp. 176.4°C/czs σ>

o / L 6 /d/dV

AP.00779

Co. No	Ex. No.	R¹	R²	R³	-X-	physical data
43	3	F	H	CH(CH₂CH₃)₂	Λ λ — N N —	mp. 145.6°C / cis
44	4	Cl	H	CH(CH₂CH₃)2	—\	mp. 156.7°C / cis
45	4	F	F	(CH₂)2CH(CH₃)2	—K	mp. 176.8°C /cis
46	3	F	F	CH(CH₂CH₃)2	/—\ —N_ K —	mp. 118.6°C/ cis
47	4	Cl	H	CH(CH₃)COCH₃	—n N — \_/	mp. 157.6°C/ cis
48	6	Cl	H	CH(CH₃)CH(OH)CH₃	—ti \\— v_y	mp. 153.4°C/c/j
135	3	Cl	H	CH(CH₃)CH₂CH₃	—N N—	cis

Table 4

c.

Ol <

'1

N-N

R⁹-<

N

I

R⁸

o u

N^XN-CH—CH2-CH3 \ ' ¹b=N CH₃

Co. No.	Ex. No.	R⁹	R⁸	physical data
49	3	cf₃	H	mp. 133.3°C
50	3	cf₃	CH₃	mp. 159.6°C
51	3	H	(CH₂)₃CH₃	mp. 173.5°C
52	3	H	CH(CH₃)2	mp. 159.1°C
53	3	LJ	ch₂ch₃	mp. 175.6°C
54	3	H	CH₂CH(CH₃)2	mp. 186.4°C
55	3	H	(CH₂)2CH₃	mp. 168.5°C
56	3	CH₃	ch₃	mp. 170.0°C
57	3	nh₂	H	-
58	3	OH	CH₃	-
59	3	OH	CH(CH₃)₂	-

AP . Ο Ο 7 7 8

-20Table 5

v --.-¾

Co. No.	Ex. No.	R¹	R2	R³	A-B	physical data
60	3	Cl	H	CH(CH₃)CH₂CH₃	CH=N	mp. 147.7°C
61	3	Cl	H	CH₂CH(CH₃)2	CH=N	mp. 159.4°C
62	4	F	F	CH(CH₃)CH₂CH₃	CH=N	mp. 100.6°C
63	4	F	H	CH(CH₃)CH₂CH₃	CH=N	mp. 138.8°C
64	3	F	H	CH(CH₂CH₃)₂	CH=N	mp. 132.3°C
65	3	F	F	CH(CH₂CH₃)₂	CH=N	mp. 120.4°C
66	3	F	H	cyclo(C5H9)	CH=N	mp. 163.0°C
67	□	F	F	cyclo(C5H9)	CH=N	mp. 150.7°C
68	3	Cl	H	CH(CH₃)₂	N=CH	mp. 170.1°C
69	3	Cl	H	CH(CH₃)CH₂CH₃	N=CH	mp. 176.2°C
70	4	F	H	CH(CH₃)CH₂CH₃	N=CH	mp. 157.3°C
71	4	F	F	CH(CH₃)CH₂CH₃	N=CH	mp. 162.4°C
72	4	F	F	cycloiCsHg)	N=CH	mp. 183.3°C
73	4	F	F	CH(CH₂CH₃)₂	N=CH	mp. 158.9°C
74	J	F	H	cyclo(C5H9)	N=CH	mp. 201.2°C
75	3	F	H	CH(CH₂CH₃)₂	N=CH	mp. 117.4°C

δρ/ρ/ a 7 / η η o fi ft

Table 6

CH₂-0

AP .00779

Co. No.	Ex. No.	R9	R⁸	R¹	A-B	R³	physical data
76	3	1^H	H	a	CH=N	CH(CH₃)CH₂CH₃	mp. 179.6°C
77	3	H	CH2CH3	a	CH=N	CH(CH₃)CH₂CH₃	mp. 119.3°C
78	3	CH2CH3	(CH₂)₂CH₃	a	CH=N	CH(CH₃)CH₂CH₃	mp. 97.8°C
79	3	H	(CH₂)₃CH₃	a	CH=N	CH(CH₃)CH₂CH₃	mp. 108.6°C
80	3	H	(CH₂)₂CH₃	Cl	CH=N	CH(CH₃)CH₂CH₃	mp. 87.3°C
81	3	CH₃	ch₃	Cl	CH=N	CH(CH₃)CH₂CH₃	mp. 85.6°C
82	5	H	CH(CH₃)2	Cl	CH=N	CH(CH₃)CH₂CH₃	mp. 141.2°C
83	3	H	H	Cl	N=CH	CH(CH₃)CH₂CH₃	mp. 160.1 °C
84	3	H	H	a	N=CH	CH₂CH(CH₃)2	mp. 160.6°C
85	5	H	CH(CH₃)₂	Cl	N=CH	CH(CH₃)CH₂CH₃	mp. 134.9°C
86	3	H	H	F	CH=N	CH(CH₃)CH₂CH₃	mp. 101.3°C
87	3	H	ch₃	Cl	N=CH	CH₂CH(CH₃)2	mp. 154.3°C
114	3	H	ch₃	a	CH=CH	CH(CH₃)CH2CH3	mp. 125.2°C
115	3	H	ch₃	a	CH=CH	CH(C₂H₅)CH2CH3	mp. 147.7°C
116	3	H	ch₃	a	CH=CH	cyclo(C5H9)	mp. 154.2°C
117	3	H	H	a	CH=CH	CH(CH₃)CH₂CH₃	mp. 186.8°C
118	3	H	ch₃	F	CH=CH	CH(C₂H₅)CH₂CH₃	mp. 134.1°C
119	3	H	ch₃	a	CH=N	cyclo(C5H9)	mp. 161.1°C
120	5	H	CH(CH₃)2	a	CH=CH	CH(CH₃)CH₂CH₃	mp. 137.5°C
121	3	H	ch₃	F	CH=CH	cyclo(C5H9)	mp. 166.2°C

Table 7

N-N '1

N

V

N^X

I

R⁷

Co. No.	Ex. No.	R⁷	physical data
88	3	ch₃	-
89	3	phenyl	-

AP .ο Ο 7 7 9

-22Table 8

ί·4

Co. No.	Ex. No.	A-B	physical data
90	3	C(CH₃)=N	mp. 98.3°C / 1/2 H₂0
91	3	C(CH₃)₂CO	mp. 96.0°C
92	3	CO-C(CH₃)2	mp. 127.1 °C
93	4	CH=CH	mp. 171.8°C
94	4	CH₂-CH₂	mp. 147.3°C

Table 9

ΑΡ/Ρ/ 97 / 0 0 968

Co. No.	Ex. No.	R12	A-B	R²	physical data
95	3	ch₃	CH=N	CH(CH₃)CH₂CH₃	mp. 134.2°C
96	3	ch₃	CH=N	CH₂CH(CH₃)₂	mp. 164.9°C
97	Π 0	H	CH=N	CH(CH₃)CH₂CH₃	-
98	n 0	ch₃	N=CH	CH(CH₃)2	mp. 187.7°C
99	0 0	ch₃	N=CH	CH(CH₃)CH₂CH₃	mp. 150.4°C
100	3	ch₃	N=CH	CH₂CH(CH₃)2	mp. 146.8°C

-23Table 10

Co. No.	Ex. No.	R5	R⁶	physical data
101	3	H	H	mp. 159.6°C
102	3	ch₃	ch₃	mp. 157.4°C
103	3	nh₂	nh₂	mp. 248.5°C

Table 11

AP/P/ 97 / 0 0 9 68

Co. No.	Ex. No.	Het	A-B ....	R3	physical data
104	1³	5-methy!-1,3,4-thia diazol-2-yl	CH=N	CH(CH₃)CH₂CH₃	-
105	3	2-pyridinyl	CH=N	CH(CH₃)CH₂CH₃	mp. 154.1°C
106	3	4-pyridinyl	CH=N	CH(CH₃)CH₂CH₃	mp. 174.9°C
107	3	4-methyl-2-oxazolyl	CH=N	CH(CH₃)CH₂CH₃	mp. 115.3°C
108	3	2-thiazolyl	CH=N	CH(CH₃)CH₂CH₃	mp. 158.6°C
109	3	4-oxo-2-thiazolyl	CH=N	CH(CH₃)CH₂CH₃	-
110	3	2-thiazolyl	N=CH	CH(CH₃)CH₂CH₃	mp. 157.8°C
111	3	2-thiazolyl	N=CH	CH₂CH(CH₃)₂	mp. 167.9°C
112	5	(1 -methylethy 1)-2//l,2,4-triazol-3-yl	CH=N	CH(CH₃)CH₂CH₃	mp. 128.8°C
113	5	(l-methylethyl)-l//- l,2,4-triazol-3-yl	N=CH	CH(CH₃)CH₂CH₃	mp. 150.0°C
122	3	4-methy 1-4//-1,2,4triazol-3-yl	CH=CH	CH(C₂H₅)CH₂CH₃	mp. 134.4°C

AP . Ο Ο 7 7 9

Co. No.	Ex. No.	Het	A-B	R³	physical data
123	3	4-methyl-4//-1,2,4triazol-3-yl	CH=CH	cyclofCsHg)	mp. 202.8°C
124	5	(l-methylethyl)-l//- l,2,4-triazol-3-yl	CH=CH	CH(CH₃)CH₂CH₃	mp. 155.7°C
125	3	4-methyl-4//-1,2,4triazol-3-yl	CH=N	CH(C₂H₅)CH₂CH₃	mp. 123.2°C

Table 12 •LU»''

Co. No.	Ex. No.	R2	R³	A-B	physical data
126	«V 0	H	CH(CH₃)CH₂CH₃	CH=CH	mp. 175.4°C
127	3	F	CH(CH₃)CH₂CH₃	CH=CH	mp. 155.5°C
128	3	H	cyclo(C5H9)	CH=CH	mp. 192.0°C
129	3	F	cyclo(C5H9)	CH=CH	mp. 181.8-C
130	3	H	CH(C₂H₅)CH₂CH₃	CH=CH	mp. 145.5°C
131	3	F	CH(C₂H₅)CH₂CH₃	CH=CH	mp. 139.1°C
132	3	H	(CH₂)4CH₃	N=CH	mp. 153.1°C

AP/P/ 97 / 0 0 968

C. Pharmacological example

Example 7: Apolipoprotein B fapo B) inhibition test

Cultured human liver cells (HepG2-celIs), which synthesize and secrete low-density lipoproteins, were incubated overnight at 37 “C in a liquid medium containing radinactively labelled leucine. Thus radioactively labelled leucine was incorporated into the apolipoprotein B. The liquid medium was decanted and the apolipoprotein B was isolated by means of a double immunoprecipitation, i.e. first an apolipoprotein B-specific antibody (antibody i) was added to the liquid medium and subsequently a second antibody (antibody2) was added which binds specifically to the apoB-antibodyicomplex. The thus formed apoB-antibody]-antibody2 complex precipitated and was isolated by centrifuge. Quantification of the amount of apolipoprotein B synthesized

AP . Ο Ο 7 7 9

-25during the night resulted from measuring the radioactivity of the isolated complex. To measure the inhibiting activity of the test compound, that test compound was added to the liquid medium at different concentrations and the concentration of apolipoprotein B synthesized in the presence of a test compound (concentration apoB (after)) was compared to the concentration of apolipoprotein B which was synthesized in the absence of the test compound (concentration apoB(control)). For each experiment the inhibition of apolipoprotein-B formation was expressed as kJ?

% inhibition = 100 x (1 - concentration of apoB(after)/concentration apoB(control))

When more experiments were carried out for the same concentration, the median value of the inhibition calculated for these experiments was calculated. IC50-values (concentration of the drug needed to reduce apoB secretion to 50 % of the control) were also computed.

Table 13 lists the IC50-values for some of the exemplified compounds of formula (I). Exemplified compounds of formula (I) that are not listed in Table 13, and for which data is available, have an IC50-value of 1 χ IO'⁶ M or more.

AP/P/ 97 / 00968

Table 13

Comp. No.	IC50 (χ IO-⁸ M)
1	9.2
2	4.7
3	9.1
4	26
5	20
6	12
7	7.9
8	13
9	11
12	19
13	51
15	4.8
18	4.1
22	7.1
23	14
24	5.8

Comp. No.	IC50 (χ 10-⁸ M)
54	7.9
55	7.8
56	23
58	31
60	4.6
61	8.1
62	19
63	4.6
64	16
65	29
66	13
67	18
68	8.1
69	2.6
71	12
72	19

Comp. No.	IC50 (x 10-8 M)
89	51
93	2.7
94	19
95	1.8
96	4.7
98	2.0
99	1.5
100	2.1
101	16
102	37
105	9.9
106	88
107	4.5
108	2.6
110	2.7
111	6.2

_Ap .ΰ ϋ 7 7 9

Comp. No.	IC50 (χ 10-⁸ M)	Comp. No.	IC50 (χ IO'⁸ M)	Comp. No.	IC50 (χ IO-⁸ M)
28	9.7	73	18	112	98
32	18	74	14	113	3.0
33	9.1	75	12	114	5.3
35	7.7	76	2.4	115	5.7
37	23	77	7.1	116	5.8
38	6.5	78	5.3	117	1.6
40	2.3	79	4.6	118	9.1
43	11	80	7.2	119	4.6
44	5.1	81	4.9	121	14
49	85	82	3.1	122	8.8
50	26	83	1.5	123	7.4
51	4.7	84	2.8	126	14
52	25	87	6.9	128	18
53	8.4	88	45	130	If

CO σ>

o

Λ '-JS*

D. Composition examples

The following formulations exemplify typical pharmaceutical compositions in dosage unit form suitable for systemic or topical administration to warm-blooded animals in accordance with the present invention.

Active ingredient (A.L) as used throughout these examples relates to a compound of formula (I), a A-oxide form, a pharmaceutically acceptable acid addition salt or a stereochemically isomeric form thereof.

Example 8 : Oral solutions g of methyl 4-hydroxybenzoate and 1 g of propyl 4-hydroxybenzoate are dissolved in 4 1 of boiling purified water. In 3 1 of this solution are dissolved first 10 g of 2,3-dihydroxybutanedioic acid and thereafter 20 g of the A.I. The latter solution is combined with the remaining part of the former solution and 121 of 1,2,3-propanetriol 15 and 3 1 of sorbitol 70% solution are added thereto. 40 g of sodium saccharin are dissolved in 0.5 1 of water and 2 ml of raspberry and 2 ml of gooseberry essence are added. The latter solution is combined with the former, water is added q.s. to a volume of 201 providing an oral solution comprising 5 mg of the A.I. per teaspoonful (5 ml).

The resulting solution is filled in suitable containers.

p^ £

Cu <

AP · Ο θ ^{7 7 9}

-27Example 9 : Capsules g of the A.I., 6 g sodium lauryl sulfate, 56 g starch, 56 g lactose, 0.8 g colloidal silicon dioxide, and 1.2 g magnesium stearate are vigorously stirred together. The resulting mixture is subsequently filled into 1000 suitable hardened gelatin capsules, each comprising 20 mg of the A.I..

Example 10 : Film-coated tablets

Prep.araij.on..of..uhlet.c.o.re

A mixture of 100 g of the A.I., 570 g lactose and 200 g starch is mixed well and 10 thereafter humidified with a solution of 5 g sodium dodecyl sulfate and 10 g polyvinylpyrrolidone (Kollidon-K 90) in about 200 ml of water. The wet powder mixture is sieved, dried and sieved again. Then there are added 100 g microcrystalline cellulose (Avicel) and 15 g hydrogenated vegetable oil (Sterotex). The whole is mixed well and compressed into tablets, giving 10.000 tablets, each comprising 10 mg of the active 15 ingredient.

.Coating

To a solution of 10 g methyl cellulose (Methocel 60 HG) in 75 ml of denaturated ethanol there is added a solution of 5 g of ethyl cellulose (Ethocel 22 cps) in 150 ml of dichloromethane. Then there are added 75 ml of dichloromethane and 2.5 ml

1,2,3-propanetriol. 10 g of polyethylene glycol is molten and dissolved in 75 ml of dichloromethane. The latter solution is added to the former and then there are added 2.5 g of magnesium octadecanoate, 5 g of polyvinylpyrrolidone and 30 ml of concenJ· trated colour suspension (Opaspray K-1-2109) and the whole is homogenated. The . tablet cores are coated with the thus obtained mixture in a coating apparatus.

Example 11 : Injectable solution

1.8 g methyl 4-hydroxybenzoate and 0.2 g propyl 4-hydroxybenzoate were dissolved in about 0.5 1 of boiling water for injection. After cooling to about 50°C there were added while stirring 4 g lactic acid, 0.05 g propylene glycol and 4 g of the A.I. The solution was cooled to room temperature and supplemented with water for injection q.s. ad 11 volume, giving u. solution of 4 mg/ml of A.I. The solution was sterilized by filuaiion (U.S.P. XVII p. 811) and filled in sterile containers.

Claims

Claims

1. A compound of formula a /V-oxide, a stereochemically isomeric form, or a pharmaceutically acceptable acid addition salt thereof, wherein A and B taken together form a bivalent radical of formula :

-N=CH- (a), -CH=N- (b), -CH2-CH2- (c), -CH=CH- (d), -C(=O)-CH₂- (e), -CH₂-C(=O)- (f),

AP/P/ 9 7 / 0 0 9 68 in the bivalent radicals of formula (a) or (b) the hydrogen atom may be replaced by Ci.galkyl; in the bivalent radicals of formula (c), (d), (e), (f), one or two hydrogen atoms may be replaced by Ci_6alkyl;

Rtis hydrogen, Ci-galkyl or halo;

R² is hydrogen or halo;

R³ is hydrogen; Ci^alkyl; C3_6cycloalkyl; or C —galkyl substituted with hydroxy, oxo, Cj. 6cycloalkyl or aryl;

Het is a heterocycle, bound to the sulfur atom via a carbon atom, selected from the group consisting of pyridine; pyridine substituted with one or two substituents selected from Cj^alkyl, hydroxy, Ci^alkyloxy, trihalomethyl, amino, mono- or di(Ci_6alkyl)amino or aryl; pyrimidine; pyrimidine substituted with one or two substituents selected from Cpgalkyl, hydroxy, Cj^alkyloxy, trihalomethyl, amino, mono- or di(Ci_6alkyl)-amino or aryl; tetrazole; tetrazole substituted with Chalky! or aryl; triazole; triazole substituted with one or two substituents selected from C^alkyl, hydroxy, C^alkyloxy, trihalomethyl, amino, mono- or di(Ci_6alkyl)-amino; thiadiazole; thiadiazole substituted with one or two substituents selected from Ci^alkyl, hydroxy, C^alkyloxy, trihalomethyl, amino, monoor di(C|_6alkyl)amino; oxadiazole substituted with one or two substituents selected from

AP. Ο Ο 7 7 9

- 29 C'l-6alkyl, hydroxy, Cj^alkyloxy, trihalomethyl, amino, mono- or di(C]_6alkyl)amino: imidazole; imidazole substituted with one or two substituents selected from Ci.^alkyl. hydroxy, Ci^alkyloxy, trihalomethyl, amino, mono- or di(Ci.galkyl)amino; thiazole; thiazole substituted with one or two substituents selected from Ci^alkyl.

hydroxy, Ci_6alkyloxy, trihalomethyl, amino, mono- or di(C,.^alkyl)amino; oxazole; oxazole substituted with one or two substituents selected from Ci.galkvl, hydroxy, Ci.galkyloxy, trihalomethyl, amino, mono- or di(C j.^alkyl)amino, aryl is phenyl or phenyl substituted with Cj^alkyl or halo.
2. A compound according to claim 1 wherein R¹ is chloro or fluoro.
3. A compound according to claim 1 wherein R¹ is methyl.
4. A compound according to any one of claims 1 to 3 wherein the bivalent radical -A-B- is N=CH- or -CH=N-, wherein one hydrogen atom is optionally replaced by a C j.^alkyl.
5. A compound according to any of claims 1 to 4 wherein R³ is butyl, pentyl or cyclopentyl.
6. A compound according to claim 1 wherein the compound is cw-4-[4-[4-[4-[[2-(4-chlorophenyl)-2-[[(4-methyl-4/7-l,2,4-triazol-3-yl)thio]methyl]-l,3dioxolan-4-yl]methoxyjpnenyl]-1 -piperazinyl]phenyl]-2,4-dihydro-2-( 1 -methylpropyl)-3#l,2,4-triazol-3-one;

cij-2-[4-[4-[4-[[2-(4-chlorophenyl)-2-[[(4-methyl-47i-l,2,4-triazol-3-yl)thio]methyl]-l,3dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-2,4-dihydro-4-(l-methylpropyl)-3#1.2.4- triazol-3-one;

czj-2-[4-[4-[4-[[2-(4-fluorophenyl)-2-[[(4-methyl-47/-l,2,4-triazol-3-yl)thio]methyl]-l,3dioxolan-4-yl]methoxy]phenyI]-l-piperazinyI]phenyl]-4-cyclopentyI-2,4-dihydro-3//-1,2,4triazol-3-one;

cz5-2-[4-[4-[4-[[2-(4-chlorophenyl)-2-[[(4-methyl-4H-1,2,4-triazol-3-yl)thio]methyl]-1,3dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-2,4-dihydro-4-pentyl-3H-1,2,4triazol-3-one;

czs-4-(l-ethylpropyl)-2-[4-[4-[4-[[2-(4-fluorophenyl)-2-[[(4-methyl-4//-l,2,4-triazol-3yl)thio]methyl]-l,3-dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-2,4-dihydro-3H1.2.4- triazol-3-one; a pharmaceutically acceptable acid addition salt or a stereochemically isomeric form thereof.

AP/P/ 97/00968

AP.00779
7. A compound according to claim 1 wherein the compound is (-)-[2S-[2a,4a(S*)]]-4-[4-[4-[4-[[2-(4-chlorophenyl) -2-[[(4-methyl-4//-1,2,4-triazol-3yl)thio]methyl]-l,3-dioxolan-4-yl]methoxy]phenyl]-l-piperazinyl]phenyl]-2,4-dihydro-2(l-methylpropyl)-3//-l,2,4-triazol-3-one, or a pharmaceutically acceptable acid addition salt form thereof.
8. A pharmaceutical composition containing a pharmaceutically acceptable carrier and as an active ingredient a therapeutically effective amount of a compound as claimed in any of claims 1 to 7.
9. A process of preparing a pharmaceutical composition as claimed in claim 8, wherein a therapeutically effective amount of a compound as claimed in any of claims 1 to 7 is intimately mixed with a pharmaceutically acceptable carrier.
10. A compound as claimed in any one of claims 1 to 7 for use as a medicine.
11. A compound as claimed in any one of claims 1 to 7 for use as a medicine useful in the treatment of hyperlipidemia.
12. A process for preparing a compound of formula (I) as defined in claim 1, characterized in that:

a) an intermediate of formula (II), wherein -A-B and R³ are as defined in claim 1, is 0alkylated with an intermediate of formula (III), wherein R¹, R² and Het are as defined in claim 1 and W is an appropriate leaving group such as halo or a sulfonyloxy leaving group, :jS

8 9 6 0 0 / L 6 /d/dV

b) an intermediate of formula (V), wherein Het is defined as in claim 1, is reacted with an intermediate of formula (IV), wherein R¹, R², R³, -A-B- are as defined in claim 1 and wherein W is an appropriate leaving group such as halo or a sulfonyloxy leaving group;

Het-SH (V) or optionally converting the compounds of formula (I) into each other by a functional group transformation reaction; and, if desired, converting a compound of formula (I) into a therapeutically active non-toxic acid addition salt, or conversely, converting an acid addition salt into a free base form with alkali; and / or preparing IV-oxide or stereochemically isomeric forms thereof.