AP662A

AP662A - Anhydrous crystalline valaciclovir hydrochloride.

Info

Publication number: AP662A
Application number: APAP/P/1997/001058A
Authority: AP
Inventors: Carter Barry Howard; Partin Jane Muse; Varlashkin Peter Gregory; Winnike Richard Augustus; Grubb William Bayne; Conway Gregory Alan; Lake Philip George; Skinner David Michael; Whatrup David James
Original assignee: The Wellcome Foundation Ltd
Priority date: 1995-01-20
Filing date: 1996-01-19
Publication date: 1998-08-19
Also published as: SK285329B6; BG101833A; NO973326L; RS49518B; SI0804436T1; BR9606768A; DK0804436T3; HRP960024A2; IN182468B; KR100376074B1; UY25779A1; PL321326A1; YU3396A; CA2210799A1; RO118693B1; HRP960024B1; GEP20001940B; UA46001C2; MX9705462A; EA000364B1

Abstract

The invention relates to an anhydrous crystalline form of (2-[2-amino-1,6-dihydro-6-oxo-purin-9-yl)methoxy]ethyl l-valinate hydrochloride (otherwise known as valaciclovir hydrochloride)and a process for preparing it.

Description

GUANINE DERIVATIVE

The present invention relates to a specific crystalline form of the antiviral compound valaciclovir hydrochloride, and to a process for producing it.

The compound 9-[(2-hydroxyethoxy)methyl]guanine, otherwise known as acyclovir possesses potent antiviral activity and is widely used in the treatment and prophylaxis of viral infections in humans, particularly infections caused by the herpes group of viruses (see, for example, Schaeffer et al, Nature, 272. 583-585 (1978), UK patent no. 1,523,865 and US patent no. 4,199,574). However, acyclovir is poorly absorbed (A. from the gastrointestinal tract upon oral administration and this low bioavailability means that multiple high doses of oral drug may need to be administered, especially for the treatment of less sensitive viruses or infections in order to achieve and maintain effective anti-viral levels in the plasma.

The L-valine ester of acyclovir, namely (2-[2-amino-l, 6dinydro-6-oxo-purin-9-yl)methoxylethylL-vaiinate, ( otherwise known as valaciclovir) has been shown to possess much improved bioavailability whilst retaining the anti-viral properties cf ,,.-._; 25 acyclovir. A preferred form of this compound is its hydrochloride salt which is otherwise known as valaciclovir hydrochloride. The L-valinate ester of acyclovir and its salts including the hydrochloride salt are disclosed in US patent no. 4,957,924 (see particularly example 13), European patent no. 0308,065 (see particularly example IS) and Beauchamp et al. Antiviral Chemistry and Chemotherapy, 3(3), 157-164 (1992) (see particularly page 162 column 1).

We have now found that valaciclovir hydrochloride can exist in various forms, and moreover we have discovered a form of valaciclovir hydrochloride which is anhydrous and crystalline \

AP/P/ 97 / 0 1 0 58 and which surprisingly has particularly good pharmaceutical properties. It is particularly stable and essentially nonhygroscopic. 3atches cf this crystalline form can be consistently made to a high crystal form purity i.e. where the proportion of other amorphous and crystalline forms of valaciclovir hydrochloride is limited. Furthermore this anhydrous crystalline form has good storage properties and can be readily formulated into pharmaceutical compositions such as tablets and capsules.

Accordingly in a first aspect of the invention there is provided valaciclovir hydrochloride in essentially anhydrous crystalline form including substantially the following d spacing pattern ( in Angstroms ):

10.20 ± 0.08, 8.10 ± 0.06, 7.27 + 0.06, 6.08 ± 0.05, 5.83 + 0.03, 5.37 ± 0.02, 5.23 ± 0.02, 4.89 ± 0.02, 4.42 + 0.02, 4.06 ± 0.02, 3.71 ± 0.02, 3.39 + 0.02, 3.32+ 0.02, 2.91 + 0.02, 2.77 +,0.02.

Hereinafter by anhydrous crystalline form according to the invention, we mean a crystalline form having substantially the same X-ray powder diffraction pattern as shown in figures 1 to 3, or having substantially the same d spacing pattern as defined above.

Any particular crystalline form of a molecule will have its own unique d spacing pattern which can be determined from its powder X-ray diffraction pattern using the Bragg equation ηλ = 2 dsin© where: n is the order of diffraction (usually 1); λ is the wavelength of the radiation; d is the d spacing (Angstroms); and Θ is the angle of deflection of the radiation

AP . Ο Ο 6 6 2

It will be appreciated that the measured d spacings can vary slightly e.g. depending on the degree to which the powder sample is packed.

The invention relates to the anhydrous crystalline form both in pure form and in admixture with other forms of valaciclovir hydrochloride such as hydrated crystalline forms. For example in any batch containing the anhydrous crystalline valaciclovir hydrochloride, there may also be hydrated crystalline forms of the compound.

Preferably the crystal form purity in any drug batch of valaciclovir hydrochloride is at least 70% w/w, more preferably at least 80% w/w, more preferably still at least

90% w/w, and most preferably at least 95% of anhydrous crystalline valaciclovir hydrochloride (as defined above).

In an alternative method of determining crystal form purity, since the anhydrous crystalline form of valaciclovir hydrochloride is essentially free of water of hydration, the proportion of hydrate forms of valaciclovir hydrochloride in any batch of the compound can be measured by the overall water of hydration content of each batch.

Accordingly in a second aspect of the invention there is provided valaciclovir hydrochloride having a water of hydration content of not more than 3% by weight (w/w) and including substantially the X-ray powder diffraction patterns of figures 1 to 3, or substantially the same d spacing pattern shown above.

More preferably the water of hydration content is net mere than 2% w/w, still more preferably not more than 1.5% w/w, and more preferably still not more than 1% w/w, and most preferably not more than 0.5% w/w.

AP/P/ 9 7 / 0 1 0 58

-4Thls water of hydration content is measured by the Karl Fischer method which is well known in the art and is described in the 1990 U. S. Pharmacopoeia at pages 1519-1521, and the European Pharmacopoeia, second edition (1992), part 2, sixteenth fascicule at v. 3.5.6-1.

According to a further aspect, the present invention provides a process for the production of valaciclovir hydrochloride in anhydrous crystalline form which comprises treating valaciclovir hydrochloride with a solubilising solvent serving to convert an amount of valaciclovir hydrochloride into said anhydrous crystalline form having the d spacing pattern shown above in the first aspect of invention; and thereafter isolating said anhydrous crystalline form.

The invention also provides a process for the production cf valaciclovir hydrochloride in an anhydrous crystalline form having the d spacings shown above, said process comprising the steps of.

a) forming valaciclovir in solution either in free base or salt form;

b) converting said free base valaciclovir or a salt thereof (when not the hydrochloride salt) to valaciclovir hydrochloride;

c) isolating valaciclovir hydrochloride from the solution and optionally removing unbound (damp, non-solvatec) solvent leaving the valaciclovir hydrochloride in substantially dry form;

d) treating valaciclovir hydrochloride with a solubilising solvent serving to convert an amount of the optionally dried valaciclovir hydrochloride from step c) into said anhydrous crystalline form; and

AP . ο Ο 6 6 2

e) isolating said anhydrous crystalline form.

Valaciclovir may be prepared by any method known in the art, but preferably by the methods described in the references mentioned above (US patent no. 4,957,524, European patent no. 0308,065, and Beauchamp et al, Antiviral Chemistry & Chemotherapy 303, 157-164 (1992), the disclosure of US patent no. 4,957,924 being incorporated herein by reference). Preferably the process starts from acyclovir whose synthesis is described in Schaeffer et al, Nature, 272. 583-585 (1978), UK patent no. 1,523,865 and US patent no. 4,199,574). Acyclovir is firstly esterified to its L-valine ester (valaciclovir), using an optionally protected L-valine for example carbobenzyloxy-L-valine (CBZ-L-valine) in a solvent such as pyridine or DMF in the presence of a coupling agent such as N,N' -dicyclohexylcarbodiimide, optionally in the presence of a base such as 4-dimethylaminopyridine as catalyst. Protecting groups can be removed in known manner (such as by treating with formic acid in the presence of 5% palladium on carbon) following the esterification reaction. Valaciclovir in the form of the free base or a salt of another acid (e.g. the formate) can be converted into the hydrochloride salt in conventional manner, for example by treatment with hydrochloric acid in a solvent.

The synthesis cf valaciclovir hydrochloride generally leads to the formation of the compound in solution in the reaction mixture from which it may be separated and purified as a solid product. The valaciclovir hydrochloride may then optionally be dried such as by slurrying in acetone and then drying. A number of factors influence the crystalline form of the solid product and in accordance with the present invention the conditions of separation and/or subsequent processing are adjusted to produce valaciclovir hydrochloride as the anhydrous crystalline form. For example a hydrate form of valaciclovir hydrochloride can be converted to the anhydrous

AP/P/ 97/01058 crystalline form using a suitable solvent under aporooriate conditions .

Such suitable solvent which is preferably a water-soluble organic solvent, should be sufficiently solubilising and be employed in an amount to allow partial solubilisation to effect the conversion and precipitation for example from hydrated crystalline form to the desired anhydrous crystalline form of valaciclovir hydrochloride. Advantageously the solvent is eventually removed by drying under vacuum. Preferably the organic solvent is an alcohol advantageously a lower alcohol containing 1 to 4 carbon atoms or a lower ketone (containing 3 to 6 carbon atoms). Most preferably the lower alcohol is ethanol or a solvent composed substantially of ethanol, for example in the form of denatured alcohol such as SVM or industrial methylated spirits. Most preferably also, the lower ketone contains water, preferably aqueous acetone such as having a water content of about 6% to about 12% by weight. Preferably the drug is slurried in the lower ketone. Our early investigations also suggest that methanol and isopropyl alcohol should also be suitable lower alcohols.

According to one particular embodiment cf the invention, valaciclovir hydrochloride is formed in solution, for example in ethanol/water, obtained for example by the general method referred to above and the valaciclovir hydrochloride is isolated by partial removal of the solvent by distillation followed by precipitation, for example by addition of acetone. The valaciclovir hydrochloride (such as that isolated after step c above) can be separated at this stage in an unstable solvated form by filtration. This product which is in nondesired form can then optionally be dried and processed to the desired anhydrous crystalline form as described below.

The damp valaciclovir hydrochloride following the first isolation (as in step c above) is preferably dried: such as

AP . Ο Ο 6 6 2 by being slurried in acetone then filtered and the damp solid dried for example about 30’ to about 70’C to provide substantially dry valaciclovir hydrochloride. At this point, the valaciclovir hydrochloride 5 may contain a high proportion of the dihydrate form which has a theoretical water of hydration content of about 9.8%.

According to a process for forming anhydrous crystalline valaciclovir hydrochloride, the substantially dry valaciclovir hydrochloride (as obtained above) is mixed with an amount of lower alcohol such as ethanol or denatured alcohol, preferably in an amount of about 15% to 40% w/w, more preferably about 17% to 30% w/w. The mixture is then heated for example from about 50*C to 70*C for several hours. Finally the product is dried under vacuum to remove residual solvent, for example at about 50*C to 70*C.

The present invention also provides the anhydrous crystalline form of valaciclovir hydrochloride (hereinafter identified as the active compound) for use in medical therapy, e.g. in the treatment of a viral disease in an animal, e.g. a mammal such as a human. The compound is especially useful for the treatment of diseases caused by various DNA viruses , such as herpes infections, for example, herpes simplex I and 2, varicella zoster, cytomegalovirus, Epstein-Barr viruses cr human herpes virus-6 (HHV-6) as well as diseases caused by hepatitis B. The active compound can. also be used for the treatment of papilloma or wart virus infections and, may furthermore be administered in combination with other therapeutic agents, for example with zidovudine, to treat retroviral associated infections in particular HIV infections.

In addition to its use in human medical therapy, the active compound can be administered to other animals for treatment of viral diseases, e.g. to other mammals.

AP/P/97/0 1 058

The present invention also provides a method for the treatment of a viral infection, particularly a herpes viral disease, in an animal, e.g. a mammal such as a human, which comprises administering to the animal an effective antiviral amount of the active compound.

The present invention also provides the use of the active compound in the preparation of a medicament for the treatment of a viral infection.

The active compound may be administered by any route appropriate to the condition to be treated, but the preferred route of administration is oral. It will be appreciated however, that the preferred route may vary with for example the condition of the recipient.

For each of the above-indicated utilities and indications the amounts required of the active ingredient (as above defined) will depend upon a number of factors including the severity of the condition to be treated and the identity of the recipient and will ultimately be at the discretion of the attendant physician or veterinarian. In general however, for each of these utilities and indications, a suitable effective dose win be in the range 1 to 150 mg per kilogram bodyweight of recipient per day, preferably in the range 5 to 120 mg per kilogram bodyweight per day (Unless otherwise indicated, all weights of the active ingredient are calculated with respect to the free base valaciclovir). The desired dose is preferably presented as one, two, three or four or more subdoses administered at appropriate intervals throughout the day. These sub-doses may be administered in unit dosage forms, for example, containing about 50 to 2000 mg, preferably about 250, 500, 1000 or 2000 mg of active ingredient per unit dose form.

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AP. ο Ο 6 6 ί ernes are giver. tor suisance ireaiTer.; of herpes simplex virus types 1 and 2 infectior.:total daily dose of about 1 or 2 g administered a; = mo were a oav or _ 0 twice a suooress;

of herpes simplex virus types I and 2 infections:- total dailv dose about 250 to 1 g for about one to ten years (dependmo on the patient);

tnree times a cay tor cvtcmeoalovirus infections ·. treatment of varicella zoster virus infections (for exancle total daily dose about 2 g administered at 1 g seven days; suppression of total daily dose about = c administered at 2 g 4 times a day; fcr transplant patients this daily dose is administered for three to six months fcr the period at risk; and for HIV positive patients a daily dose is administered as usually indicated for improving quality of life, fcr examole for two years cr mere.

Early results now indicate that valaciclovir can be used in the effective suppression of recurrent genital herpes at a once daily dose of from about 200 mg to about 1000 mg fcr an effective treatment period. The meso likely daily dosages are 250 mg, 500 mg cr 1000 mg.

While it is possible for the active ingredient to be administered alone, it is preferable to present io as a pharmaceutical formulation. The formulation comprises the active ingredient as above defined, together with one or more pharmaceutically acceptable excipients therefor and optionally other therapeutic ingredients. The excipient is) muse be acceptable in the sense of being compatible with the ocner ingredients of the formulation and not deleterious to the recipient thereof.

The formulations include those suitable for oral administration and may conveniently be presences in unit dosage form oreoared bv ar.v of the methods we_i xr.ow pharmacy. Such methods include the step of bringing mt association che aocive ingredient wetr. me carrier wr.ee constitutes one or more accessory mgreocer.ts . In genera-.

AP/P/ 97 / 0 1 0 58 the formulations are prepared by uniformly and intimately bringing into association the active ingredient with liquid carriers or finely divided solid carriers cr both, and then, if necessary, shaping the product.

Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, sachets of granules or tablets (such as a swallowable, dispersible or chewable tablet) each containing a predetermined amount of the active ingredient; as a powder or granules; as a solution or a suspension in an aqueous liquid or a non-aqueous liquid; or as an oil-in-water liquid emulsion or a water-in-oil liquid emulsion. The active ingredient may also be presented as a bolus, electuary or paste.

A tablet may be made by compression or moulding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active ingredient in a free-flowing form such as a powder or granules, optionally mixed with a binder, lubricant, inert diluent, preservative, surface active or dispersing agent. Moulded tablets may be made by moulding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent. The tablets may optionally be coated or scored and may be formulated so as to provide slow or controlled release of the active ingredient therein.

Preferred unit dosage formulations are those containing a daily dose or unit daily sub-dose (as herein above recited) or an appropriate fraction thereof, of the active ingredient.

It should be understood that in addition to the ingredients particularly mentioned above the formulations of this invention may include other agents conventional in the art having regard to the type of formulation in question, for example those suitable for oral administration may include

AP. Ο Ο 6 6 2 flavouring agents cr taste masking agents.

The invention is illustrated by the following examples:

Example 1

A. 2 -Γ(2-amino-l.6-dihvdro-6-oxo-9H-purin-9-yl) methoxy]ethvl-N-Γ (benzoloxv)carbonyl1-L-valinate

CBZ-L-valine (170 g) was dissolved in dimethylformamide (DMF) (750 ml) and cooled. A cold solution of N,N-dicyclohexylcarbodiimide (DCC) (156.7 g) in DMF (266 ml) was added and stirred with cooling. Acyclovir (10.1 g) was added in a single portion, and then 4-(dimethylamino)pyridine (9.4 g) was added while maintaining cooling. The mixture was stirred cold overnight. A white precipitate. of the by-product was then removed by filtration. The filtrate was reduced in volume by vacuum distillation and the concentrate treated with water (663 ml) then heated to 70’C. The suspension was cooled to

20 *C, filtered and the solid washed with water.

The damp, crude material was then purified by recrystallisation from denatured alcohol (1.2 litres) to afford the title compound as a damp white crystalline solid (281.5 g).

B. 2-Γ ( 2-amino-l, 6-dihydro-6-oxo-9H-ourin-9-yl'>

methoxy]ethyl-L-valinate hydrochloride

2- [ (2-amino-l,6-dihydro-6-oxo-9H-purin-9-yl)methoxv]ethyl-N[(benzyloxy)carbonyl]-L-valinate (175 g) was charged to aqueous denatured alcohol (335 ml/795 ml) and heated to reflux. The solution, was then cooled to 40*C. The suspension was treated with 5% palladium on carbon catalyst (35 g wet weight 50% wet with water) then formic acid (30.6 ml of 90% w/w) added over 1 hour. The reaction mixture was stirred for a further 1 hour then a second charge of formic acid made

AP/P/ 97 / 0 1 0 58 _ ' 7 _ (19.5 ml) and the mixture filtered to remove the catalyst. The filter cake was washed with denatured alcohol and the combined filtrates were treated with concentrated hydrochloric acid (33.7 ml) and the resultant mixture was concentrated by vacuum distillation.

Acetone (1295 ml) was then added over 15 minutes and the suspension stirred for 1 hour before filtering off the product. The solid was then slurried with acetone (circa. 530 ml), refiltered and dried at 6Q*C in vacuo to give the title compound (1123 g : 81.6%).

A 15 g sample of this material was combined with denatured alcohol (circa. 7 ml), to moisten and was heated with agitation at 60 *C overnight in a closed flask to avoid loss of alcohol and maintain the dampness of the mixture. The mixture was then dried at 60 *C in vacuo to afford the product as the desired morphic form.

Physical Data:

Karl Fischer value : 0.9% w/w water.

The X-ray powder diffraction patterns of the product cf example 13 are shown in Figure 1 of the accompanying drawings.

The d spacings and further X-ray diffraction data are shown in Table 1.

-13- AP . 0 0 6 6 2

Table 1

	Peak No:	Angle	Peak
5		( degrees )	( counts;
	1	3.56	630
	2	8.62	1151
	3	9.42	87
10	4	10.86	1438
	5	12.10	835
	6	13.22	198
	7	14.49	2172
	8	15.12	455
15	9	15.90	352
	10	15.45	1969
	11	16.90	744
	12	17.33	119
	13	18.12	1013
20	14	22.71	1429
	15	20.55	256
	16	21.21	370
	17	21.83	753
	18	22.71	95
25	19	23.95	2893
	20	25.10	171
	21	26.21	1784
	22	26.89	428
	23	27.08	373
30	24	23.02	158
	25	23.27	151
	26	28.91	391
	27	29.68	191
	28	30.55	502
35	29	31.34	110
	30	31.58	98
	31	32.13	597
	32	32.96	260
	33	33.99	344
40	34	34.38	374
	35	35.12	141
	36	35.78	408
	37	33.71	101
45	I/Imax -	(peak height/max.

peak ht) x 100

d Spacing parrern (A)	Error in d (± A)	I/Zmac (*)
24.8	0.5	24
10.25	0.08	39
9.38	0.07	3
8.14	0.06	49
7.31	0.06	28
6.69	0.05	6
6.11	0.05	75
5.85	0.03	15
5.57	0.02	12
5.38	0.02	68
5.24	0.02	25
5.11	0.02	4
4.89	0.02	35
4.43	0.02	49
4.32	0.02	8
4.19	0.02	12
4.07	0.02	26
3.91	0.02	3
3.71	0.02	100
3.54	0.02	5
3.40	0.02	61
3.31	0.02	14
3.29	0.02	12
3.18	0.02	5
3.15	0.02	5
3.09	0.02	13
3.01	0.02	6
2.92	0.02	17
2.65	0.02	3
2.83	0.02	3
2.78	0.02	20
2.72	0.02	8
2.64	0.02	11
2.61	0.02	12
2.55	0.02	4
2.44	0.02	14
2.32	0.02	3

AP/P/ 97 / 0 1 0 58

The powder sample used to produce the above X-ray diffraction data was prepared by an equivalent method as the powder sample used to produce the X-ray diffraction date of table 2 (described hereinafter) except that for the above data the following preparation was used to prepare the powder sample.

The sample was prepared by milling 1 g of sample in a plastic cup using two acrylic balls for 5 minutes with a Chemplex Spectromill. The samples were then back packed against a glass slide to a depth of 2 mm.

The X-ray diffraction scan was obtained using a Scintag PADV diffractometer in the step scan mode at 0.02’ per step and a 10 second count per step. The sample holder was spun at 1 rotation per second during the scan. Additional setting as described below.

X-ray generator: 45 kV, 40 mA

Radiation: Copper K alpha radiation

Fixed divergent slit: 1 mm

Incident scatter slit: 2 mm

Diffracted scatter slit: 0.5 mm

Receiving slit: 0.3 mm

Goniometer radius: 235 mm

Detector: Scintillation with a graphite monochromator.

The peak intensities are reported as absolute counts of the peak top. The intensity units on the X-ray diffraction plot are counts/sec. The absolute counts = counts/sec x count time = counts/sec x 10 sec. The peak intensities in the table have been corrected for background and copper K alpha II X-ray wavelength contribution.

Slight variations in d spacings are expected based on the specific diffractometer employed and the analyst's sample preparation technique. More variation is expected fcr the relative peak intensities. Identification of the exact crystal form of a drug should be based primarily on observed

AP.00662 d spacings with lesser importance placed cn relative peak intensities. To identify the anhydrate crystal form of valaciclovir hydrochloride, the fifteen most intense diffraction peaks are reasonably characteristic. These peaks occur at 10.25 ± 0.08, 8.14 ± 0.06, 7.31 r 0.06, 6.11 ± 0.05, 5.85 ± 0.03, 5.38 ± 0.02, 5.24 * 0.02, 4.89 ± 0.02, 4.43 * 0.02, 4.07 ± 0.02, 3.71 ± 0.02, 3.40 ± 0.02, 3.31 ± 0.02, 2.92 ± 0.02, and 2.78 ± 0.02 angstroms. The error in determining d spacings decrease with increasing diffraction scan angle or decreasing d spacing. The error of the 10.25 angstroms peak would be approximately ± 0.03 angstrom and the error of the 2.78 angstroms peak would be approximately ±0.01 angstrom for a reasonably aligned diffractometer and reasonably prepared sample.

/ 15

The first peak In the line listing at 3.56 degrees is due to a hydrated crystal phase of valaciclovir hydrochloride and not due to the anhydrate crystal form.

In a further sample of anhydrous crystalline valaciclovir hydrochloride, the following d spacings were obtained:

AP/P/ 9 7/01058

	10.20,	8.10,	7.27,	6.58,	6.08,	5.83,	5.56, 5.37, 5.23, 5.10,
	4.89,	4.42,	4.31,	4.18,	4.06,	3.91,	3.71, 3.64, 3.54, 3.39,
25	3.35,	3.32,	3.28,	3.22,	3.13,	3.14,	3.C8, 3.00, 2.97, 2.91,
	2.85,	2.77,	2.70,	2.63,	2.60,	2.55,	2.44, 2.42, 2.37, 2.32.

The fifteen most characteristic peaks are 10.20 ± 0.08, 8.10 ± 0.05, 7.27 ± 0.04, 6.08 ± 0.03, 5.83 ± 0.03, 5.37 ± 0.02,

5.23 ± 0.02, 4.89 ± 0.02, 4,42 ± 0.02, 4.06 ± 0.01, 3.71 ±

0.01, 3.39 ± 0.01, 3.32 ± 0.01, 2.9 ± 0.01, and 2.77 ± 0.01.

Example 2

A. 2-Γ ( 2-amlno-l, 5-dihydro-6-oxo-9H-purin-9-vl ) methoxvlethvl-N-^r (benzvloxv)carbonvl1-L-valinate

CBZ-L-valine (167 g) was dissolved in dimethylformamide (DMF) (750 ml) and cooled. A cold solution of N,N-dicyclohexylcarbodiimide (DCC) (153.5 g) in DMF (266 ml) was added followed by acyclovir (111.7 g) in a single portion. 4(dimethyl-amino) pyridine (9.4 g) was then added and the mixture stirred cold overnight. A white precipitate of the by-product was then removed by filtration. The filtrate was reduced in volume as before to give the title compound (215.3 g) ·

B. 2-Γ(2-amlno-l,6-dihydro-6-oxo-9H-purin-9-yl) methoxy]ethyl-L-vallnate hydrochloride

2-[ (2-amino-1,6-dihydro-6-oxo-9H-purin-9-yl)methoxy]ethyl-N[(benzyloxy) carbonyl]-L-valinate (200 g) was charged to aqueous denatured alcohol (382 ml/908 ml) and heated to reflux to dissolve solids. The solution was then cooled to 40’C. The suspension was treated with a 50% w/w paste of 5% palladium on carbon catalyst and water (40 g) then formic acid (96% w/w : 32.8 ml) added over 1 hour. The reaction mixture was stirred for a further 1 hour then a second charge cf formic acid made (20.88 ml) and the mixture filtered to remove the catalyst. The filtrate was treated with concentrated hydrochloric acid (38.56 ml) and the resultant mixture distilled and concentrated under vacuum.

Acetone (1480 ml) was then added over 15 minutes and the suspension stirred for 1 hour before filtering off the product. The solid was then slurried with acetone (ca. 500 ml), refiltered and dried at 60’C in vacuo to give the title compound (137.75 g : 87.6%).

AP.00662

A 10 g sample cf this material was combined with denatured alcohol (3.5 ml), heated at 60’C for several hours and the solvent then removed in vacuo to afford the product as the desired mcrphic form.

Result: Valaciclovir hydrochloride in the anhydrous crystalline form was obtained substantially free of other forms of valaciclovir hydrochloride (i.e. in more than about 90% w/w anhydrous crystal form purity).

Physical Data:

The X-ray powder diffraction patterns of the product of example 13 are shown in Figures 2 and 3 of the accompanying drawings in which :Fig 2 is a linear plot X-ray diffractogram; and

Fig 3 is a square root plot X-ray diffractogram.

The d spacings and further X-ray diffraction data are shown in Table 2.

Table 2 25

AP/P/ 9 7 / 0 1 0 58

Peak	No: Angle (degrees)	Peak (counts )	d Spacing pattern (A)	I/Imax (%)
1	3.62	2673	24.40	35
2	7.21	119	12.26	2
3	8.64	1910	10.22	25
4	9.43	180	9.37	2
5	10.86	2652	8.14	35
6	12.12	734	7.30	10
7	13.24	615	6.68	8
8	13.77	106	6.42	1
9	14.50	2333	6.11	31
10	15.14	635	5.35	8
11	15.89	511	5.57	7
12	16.44	2652	5.39	35

uir

Table 2	- continued
Peak No	: Angle ( degrees ) (	Peak counts )	d Spacing oattern * (A)	Iz'Imax (%)
13	16.90	1267	5.24	17
14	17.33	475	5.11	6
15	'18.13	1548	4.89	22
16	20.05	2172	4.43	28
17	20.56	640	4.32	8
18	21.20	1096	4.19	14
19	21.78	2034	4.08	27
20	21.90	1384	4.06	18
21	22.66	729	3.92	10
22	23.94	7621	3.71	100
23	24.39	1624	3.65	21
24	25.11	967	3.54	13
25	25.86	2460	3.44	32
26	26.21	5127	3.40	67
27	26.82	1892	3.32	25
28	26.89	1927	3.31	25
29	27.19	1429	3.28	19
30	27.99	1156	3.18	15
31	28.35	1076	3.15	14
32	28.87	1722	3.09	23
33	28.94	1529	3.08	20
34	29.62	1274	3.01	17
35	30.56	1673	2.92	22
36	31.30	999	2.86	13
37	32.25	2570	2.77	34
38	33.04	1376	2.71	18
39	34.00	1806	2.63	24
40	34.45	1225	2.60	16
41	35.13	1149	2.55	15
42	36.77	1600	2.44	21
43	38.01	576‘	2.37	8
44	38.76	729	2.32	10
45	39.52	524	2.28	7
46	40.70	751	2.22	10
47	41.28	870	2.19	11
48	41.88	686	2.16	9
49	42.47	718	2.13	9
50	43.40	543	2.08	7
51	44.53	729	2.03	10
The dif	fraction pat	terns of	the product of	example

generated on a Phillips PW1800 Automatic X-ray Powder Diffractometer using a scan of 2 to 45 2Θ with step intervals z

AP. Ο Ο 6 6 2 of 0.02 degrees and an integration time of 4 seconds per step.

Generator settings: 40 KV, 45 mA, Cu alpha 1,2 wavelengths: 1.54060, 1.54439 A; Step site, sample time: 0.020 deg, 4.00 s, 0,005 deg/s; monochromator used: yes; divergence slit: automatic (irradiated sample length: 10.0 mm); peak angle range: 2.000 - 45.000 deg; range in D spacing: 44.1372 2.01289 A; peak position criterion: top of smoothed data; cryst peak width range: 0.00 - 2.00 deg; minimum peak significance: 0.75 maximum intensity: 7621 cts, 1905.3 cps.

The powder sample was prepared as follows:

A 1 gram portion of valaciclovir hydrochloride was transferred 15 to a Retsch 10 ml polystyrol container ref 31-762 containing acrylic balls ref 26-253 and was then ground to a very fine powder using a Retsch MM2 miser mill set at 100% power for five minutes. The ground powder was back loaded into a Philips PW1811/10 sample holder which had been placed inverted on a perfectly smooth surface (e.g. that afforded by a glass plate or a highly polished metal sheet). The powder was then packed into the holder and further powder added and packed until the holder was full. A Philips PW 1811 00 bottom plate was then clamped into the holder and the entire assembly was then inverted before removing the glass/metal plate in an upwards direction to reveal the smooth sample surface which was flush with that of the holder.

As illustrated above, crystalline forms of valaciclovir hydrochloride can be characterised by their X-ray powder diffraction pattern. Figures 1 to 3 show diffractograms of the anhydrous crystalline form of valaciclovir hydrochloride as a linear plot (Figures 1 and 2) and a square root plot (Figure 3). In each case the diffractogram displays count rate (intensity of the diffracted peak) against diffraction angle 2Θ. The linear plot allows easy rationing of the peak intensifies whereas the square root plot attenuates small

AP/P/ 9 7/01058

-20peaks thereby emphasising the main peaks in the diffraction pattern.

Example 3

Hygroscopicity and Stability Measurements of anhydrous

Crystalline Valaciclovir Hydrochloride

Hygroscopicity: An integrated microbalance system was used to measure equilibrium water sorption profiles (Model MB300G, VTI Corp.), weighing about 20 mg of valaciclovir hydrochloride, and using the following conditions:

Drying parameters:Temperature: 65*C; heating rate: 10’C/min; equilibrium criteria: 5 pg; sample interval: 5 min Sorption parameters :Temperature: 25’C; equilibrium criteria: 5 pg; sample interval: 5 min.

Data Collection Interval: 2 min

The water sorption/desorption were isotherms noted in Figure

4.

The isotherm shows that only a small amount of water is absorbed. This absorption is, in fact, due to the presence of hydrated crystal form in the sample which is consistent with the 0.9% w/w water measured by the Karl Fischer titration.

The effect of the small amount of this hydrate can be seen more clearly by comparing with the isotherm of figure 5 which was generated with substantially 100% anhydrate crystal form.

X-ray powder diffraction: No change between the initial powder pattern and the patterns of material stored for 4 weeks at 40*C, ambient temp/60% RH (relative humidity), and 40*C/75%

AP.00662

RH.

% water :

	Initial:	0.9
5	40’C 2 weeks:	0.Θ
	40*C 4 weeks:	0.8
	ambient T/60% RH, 2 weeks:	1.0
	ambient T/60% RH, 4 weeks:	0.9
	40*C/75% RH, 2 weeks:	1.1
10	40*C/75% RH, 4 weeks:	1.1

Four weeks chemical bulk stability data:
	% w/w on anhydrous basis
15
		INITIAL	40’C	ambient	40*C/
					T/60% RH	75%Ri
	L-valine:	ND ( <	0.1%) )	ND	ND	ND
20	2-hydroxyethyl vaiinate
	hydrochloride:	ND (<	0.1%)	ND	ND	ND
	Acyclovir:	0.9		0.9	0.9	0.9
	Guanine:	ND ( <	0.1%)	ND	ND	ND
	D-isomer of
25	valaciclovir:	1.1		1.2*	1.1	1.2*
	Valaciclovir HCI:	97.3		97.8	98.2	97.5

AP/P/ 97/01058

ND « none detected

Data rounded to nearest 1.1% w/w

There is no significant increase in 2-hydroxyethyl vaiinate hydrochloride; the actual values between the initial and 4 week time points are within 0.03% of each other (which is within experimental error).

Example 4

Further hygroscopicity and stability studies were carried out on substantially 100% anhydrate crystal form at 3O’C/75% RH (12 months) and 4O’C/75% RH (6 months).

Samples stored at 30*C/75% RH and 4O’C/75% for 12 months and 6 months, respectively, showed no significant change in moisture content (Karl Fischer) or crystallinity (as measured by X-ray powder diffraction). Using the integrated microbalance system, no more than 0.5% w/w water is absorbed at 25’C at relative humidifies of up to 90%. Furthermore, a separate sample stored for 2.5 months at 25’C and 75% RH corroborated the moisture content measured by the integrated microbalance system, that is, about 0.3% moisture at 75% RH.

The results indicate that the anhydrous crystalline form of valaciclovir hydrochloride is chemically and physically stable.

These characteristics give the anhydrous crystalline form good formulation and storage properties, and assist in obtaining in a highly reproducible manner, batches of high crystalline form purity.

,23. AP. 0 0 6 6 2

Example 5: Tablet Formulation

The following formulations was prepared as follows using anhydrous crystalline valaciclovir.

Example	5	Per Batch (kg)
Ingredients	mg/ tablet	w/w
valaciclovir hydrochloride*	615	65.74	5.289
lactose	205	21.91	1.763
microcrystalline cellulose (Avicel PH101) ( intragranular)	75	8.02	0.6450
povidone k30	18	1.92	0.1548
crospovidone (intragranular)	18	1.92	0.1548
colloidal silicon dioxide (Aerosil 200)	0.9	0.10	0.002598
magnesium stearate	3.6	0.38	0.03096
TOTAL WEIGHT	935.5	100

AP/P/ 97 / 0 1 0 58 *bulk density 0.45 g/cc after 50 taps (anhydrous crystalline form)

Example 6: Tablet Formulation

Example	6	Per Batch (kg)
Ingredients	mg/ tablet	w/w
valaciclovir hydrochloride*	576.5	82.3	0.9973
crospovidone (intragranular)	14.0	2.0	0.02422
povidone k90 (intragranular)	14.0	3.1	0.03806
crospovidone (extragranular)	14.0	2.0	0.11200
microcrystalline cellulose (Avicel PH101) (extragranular)	70.0	10.0	0.05600
colloidal silicon dioxide (CAB-O-SIL, M-5®) (extragranular)	2.0	0.3	0.00160
magnesium stearate (extragranular)	4.0	0.6	0.00320
TOTAL WEIGHT	702.5	100.0	1.13158

*bulk density 0.60 g/cc after 50 taps (anhydrous crystalline form); Karl Fischer water content = 0.4.

-25- AP . 0 0 6 6 2

Tablet Preparation For Example 5:

Step 1. The following ingredients as shown were sifted with a hand screen as shown.

Mesh valaciclovir hydrochloride lactose microcrystalline cellulose povidone K30 crospovidone

Mesh magnesium stearate colloidal silicon dioxide (CSD)

Step 2. The 30 mesh sifted ingredients from Step 1 were then blended, excluding the povidone, in a 1 cubic foot V-shell blender for 10 minutes.

Step 3. 1.540 kg of SD3A alcohol (ethanol denatured with 5% methanol) was then mixed with 0.6600 kg of purified water and the screened povidone, 0.1548 kg, was dissolved in 0.6192 kg of the mixed solvents by hand stirring.

Step 4. The blended powders from Step 2 were then granulated in a 1 cubic foot Littleford Lodige mixer by adding the dissolved povidone while mixing. 1.315 kg of more mixed solvent was added and the mixture massed for seven minutes total as shown below.

Ploughs 7 min Choppers 6.5 min

API9I 97/01016

-2όStep 5. The granule from Step 4 was then dried in a Fluid Bed Dryer (Glatt GPCG5) with an inlet air temperature of 50 *C to any acceptable moisture content of approximately 1.0 to 3.0% L.O.D.

Step 6. The material from Step 5 was then sifted using a Fitz Mill Model M fitted with a 30 mesh screen, with knives forward, operating at medium speed.

Step 7. The screened a magnesium stearate from Step 1, was added to the material from Step 6 and blended for 5 minutes using the blender from Step 2.

-ί.

Step 8. Lubricated granule 2.650 kg (from Step 7) was 15 weighed and the sifted CSD, from Step 1, added then dispersed by hand and the mixture blended for 5 minutes in the blender from Step 2. The mixture was compressed to form tablets on a Manesty Beta Press fitted with oval tooling, 19.1 mm x 10.2 mm, at a compression weight of approximately 935.5 mg.

AP.00662

Tablet Preparation For Example 6:

Step 1. The core ingredients were sifted with a 20 mesh hand screen, and then blended in an appropriately sized V-shell blender for 10 minutes.

Step 2. The blended powders from Step 1 were then granulated in a 10 litre high shear mixer (modelSPl) by adding pure water while mixing.

Approximately 11-14% water, w/w of the core ingredients was then added and the mixture massed for 3 to 4¼ minutes.

Step 3. The granule from Step 2 was dried in a tray O 15 (examples 5, 6 and 7) or vacuum (examples 3 and 4) drier (model-SPl) at a temperature of 50*C to an acceptable moisture content of approximately 1.0 to

2.0 % L.O.D.

Step 4. The remaining ingredients were sifted through a 20 mesh screen and added to the core ingredients of step 3, and then the mixture was sifted using a Comil Model 197 AS fitted with a 0.062” screen.

Step 5

Step 6

The mixture was then blended in an appropriately sized V-shell blender for 5 minutes.

The blended granule from Step 5 was compressed on a Manesty Beta Press fitted with capsule shaped tooling, 18.25 mm x 7.14 mm, at a compression weight of approximately 700 mg and a compression force of about 14.5 to 18 kN.

AP/P/ 97 / 0 1 0 58

Step 7 The tablet can then optionally be film coated 35 using standard methods such as using white colour concentrate, methylhydroxypropykellulose, titanium dioxide, polyethylene glycol and polysorbate.

Claims

1. Valaciclovir hydrochloride in anhydrous crystalline form including the following d spacing pattern (in

5 Tingstroms) :

10 .20 ± 0.08, 8.10 ± 0.06, 7.27 ± 0.06, 6.08 ± 0.05, 5. 83 ±0.03, 5.37 ± 0.02, 5.23 + 0.02, 4.89 + 0.02, 4 . 42 ± 0.02, 4.06 ± 0.02, 3.71 ± 0.02, 3.39 + 0.02, 10 3. 32 ± 0.02, 2.91 ± 0. .02, 2.77 ± 0. 02

Crystalline valaciclovir hydrochloride as claimed in Claim 1 and having a water of hydration content of not more than 3% w/w.

Crystalline valaciclovir hydrochloride as claimed in

Claim 2 having a water of hydration content of not more than 2% w/w.

20 4. Crystalline valaciclovir hydrochloride as claimed in

Claim 3 having a water of hydration content of not more than 1% w/w.

5. Crystalline valaciclovir hydrochloride having

25 substantially the same X-ray diffraction pattern as shown in Figures 1 to 3.

6. A process for the production of valaciclovir hydrochloride in anhydrous crystalline form including

30 the d spacings as defined in Claim 1, said process comprising treating valaciclovir hydrochloride, with 15% to 40% w/w of an alcohol having 1 to 4 carbon atoms or with a ketone containing from 3 to 6 carbon atoms, to convert an amount of said valaciclovir

35 hydrochloride into said anhydrous crystalline form;

and then isolating said anhydrous crystalline form.

85010//6 /d/dV

P5412KZ-WO - 16/05/S6

AP.00662

7. A process for the production of valaciclovir hydrochloride in an · anhydrous crystalline form including the d spacings as defined in Claim 1, said process comprising the steps of:

a) forming valaciclovir in solution either in free base or salt form;

b) converting said free base valaciclovir or salt

10 thereof to valaciclovir hydrochloride;

c) isolating valaciclovir hydrochloride from the solution and optionally removing unbound solvent leaving the valaciclovir hydrochloride in

15 substantially dry form;

d) treating valaciclovir hydrochloride, with 15% to

40% w/v; of an alcohol having 1 to 4 carbon atoms or with a ketone containing from 3 to 6 carbon

20 atoms, to convert an amount of said optionally dried valaciclovir hydrochloride into said anhydrous crystalline form; and

e) isolating said anhydrous crystalline form.

A process as claimed in Claim 7, wherein the alcohol is ethanol or a solvent composed substantially of ethanol.

AP/P/ 9 7/01058

30 9. A process as claimed in Claim 8, wherein the alcohol is ethanol or a solvent composed substantially of ethanol and is added in a range of about 17% by weight to about 30% by weight of substantially dry valaciclovir hydrochloride.

10. A process according to any one of Claims 7 to 9, wherein after the alcohol is added as solubilising solvent, the mixture is heated.

P5412N2-WO - 16/05/98

- 30 11. A process according to Claim 9, wherein said mixture is heated from about 50°C to 7 0°C for several hours.

12. A process according to Claims 10 or 11, wherein said

5 mixture after heating is dried under vacuum at about

50°C to 70°C.

13. Crystalline valaciclovir hydrochloride as claimed in any one of the preceding claims for use in medical

10 therapy.

14. Use of valaciclovir hydrochloride as claimed in any one of Claims 1 to 5 in the preparation of a medicament for the treatment of a herpes viral

15 infection.

15. A pharmaceutical composition comprising crystalline valaciclovir hydrochloride together with a pharmaceutically acceptable carrier, wherein said

20 valaciclovir hydrochloride is valaciclovir hydrochloride as defined in any one of Claims 1 to 5.

8S 0 I 0 / L 6 /d/dV

A pharmaceutical composition as claimed in Claim 15 which is a tablet or capsule containing 50mg to 2000mg of said crystalline valaciclovir hydrochloride.

A method of preparing a pharmaceutical composition of valaciclovir valaciclovir acceptable hydrochloride hydrochloride by hydrochloride with carrier, wherein is selected to mixing crystalline a pharmaceutically said valaciclovir be valaciclovir hydrochloride as defined in any one of Claims 1 to 5.

18. The use of crystalline valaciclovir hydrochloride as 35 defined in any one of Claims 1 to 5 to reduce the hygroscopicity of a pharmaceutical dosage form of valaciclovir hydrochloride.