AP573A - Endothelin receptor antagonists. - Google Patents

Abstract

Novel indane and indene derivates are described which are endothelin receptor antagonists.

Description

ENDOTHELIN RECEPTOR ANTAGONISTS

FIELD QF INVENTION 5

The present invention relates to novel indane and indene derivatives, pharmaceutical compositions containing these compounds and their use as endothelin receptor antagonists.

Endothelin (ET) is a highly potent vasoconstrictor peptide 10 synthesized and released by the vascular endothelium. Endothelin exists as three isofonns, ET-1, ET-2 and ET-3. [Unless otherwise stated endothelin shall mean any or all of the isoforms of endothelin]. Endothelin has profound effects on the cardiovascular system, and in particular, the coronary, renal and cerebral circulation. Elevated or abnormal release of endothelin is associated with smooth muscle contraction which is involved in the pathogenesis of cardiovascular, cerebrovascular, respiratoiy and renal pathophysiology. Elevated levels of endothelin have been reported in plasma from patients with essential hypertension, acute myocardial infarction, subarachnoid hemorrhage, atherosclerosis, and patients with uraemia undergoing dialysis.

Jn vivo, endothelin has pronounced effects on blood pressure and cardiac output An intravenous bolus injection of ET (0.1 to 3 nmol/kg) in rats causes a transient dose-related depressor response (lasting 0.5 to 2 minutes) followed by a sustained, dose-dependent rise in arterial blood pressure which can remain elevated for 2 to 3 hours following dosing. Doses above 3 nmol/kg in a rat often prove fatal.

( . Endothelin appears to produce a preferential effect in the renal vascular bed. It produces a marked, long-lasting decrease in renal blood flow, accompanied by a significant decrease in GFR, urine volume, urinary sodium and potassium excretion. Endothelin produces a sustained antinatriuretic effect, despite significant elevations in atrial natriuretic peptide. Endothelin also stimulates plasma renin activity. These findings suggest that ET is involved in the regulation of renal function and is involved in a variety of renal disorders including acute renal failure, cyclosporine nephrotoxicity, radio contrast induced renal failure and chronic renal failure.

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-lb- ..Studies have shown that in vivo, the cerebral vasculature is highly sensitive to both the vasodilator and vasoconstrictor effects of endothelin. Therefore, ET may be an important mediator of cerebral vasospasm, a frequent and often fatal consequence of subarachnoid hemorrhage.

ET also exhibits direct central nervous system effects such as severe apnea and ischemic lesions which suggests that ET may contribute to the development of cerebral infarcts and neuronal death.

ET has also been implicated in myocardial ischemia (Nichols et al. Br. J. Pharm. 99: 597-601,1989 and Clozel and Clozel, Circ, Res.. 65: 1193-1200,

1989) coronary vasospasm (Fukuda fiLak, Eur, J. Pharm. 165: 301-304, 1989 and

Liischer, Circ. 83: 701,1991) heart failure, proliferation of vascular smooth muscle cells, (Takagi. Biochem & Biophys. Res. Commun.: 168: 537-543, 1990, Bobekfii al.. Am. J. Physiol. 258.408-C415,1990) and atherosclerosis, (Nakaki et al.. Biochem. & Biophvs. Res. Commun. 158: 880-881,1989, and Lerman et al.. New

Eng. J. of Med. 325: 997-1001,1991). Increased levels of endothelin have been shown after coronary balloon angioplasty (Kadel et al.. No. 2491 Circ, 82: 627,

1990) .

Further, endothelin has been found to be a potent constrictor of isolated mammalian airway tissue including human bronchus (Uchida et al.. Eur J.

of Pharm. 154:227-228 1988, LaGente, Clin. Exp. Allergy 20: 343-348, 1990; and Springall etal.. Lancet. 337: 697-701,1991). Endothelin may play a role in the pathogenesis of interstitial pulmonary fibrosis and associated pulmonary hypertension, Glard et al.. Third International Conference on Endothelin, 1993, p. 34 and ARDS (Adult Respiratory Distress Syndrome), Sanai filaL Supra, ρ. 112.

Endothelin has been associated with the induction of hemorrhagic and necrotic damage in the gastric mucosa (Whittle et al.. Br. J. Pharm. 95: 10111013,1988); Raynaud's phenomenon, Cinniniello etal.. Lancet 337: 114-115,

1991) ; Crohn's Disease and ulcerative colitis, Munch et al.. Lancet. Vol. 339,

p. 381; Migraine (Edmeads, Headache, Feb. 1991 p 127); Sepsis (Weitzberg et al..

Circ. Shock 33: 222-227, 1991; Pittet £t_ak, Ann. Sure, 213: 262-264, 1991), Cyclosporin-induced renal failure or hypertension (Eur, J. Pharmacol.. 180: 191192, 1990, Kidney Int. 37: 1487-1491,1990) and endotoxin shock and other endotoxin induced diseases (Biochem. Biophys, Res, Commun.. 161: 1220-1227, 1989. Acta Physiol. Scand. 137: 317-318, 1989) and inflammatory skin diseases.

(Clin Res. 41:451 and 484, 1993).

AP/P/ 94/00636

-2AP.005/3

Endothelin has also been implicated in preclampsia of pregnancy. Clark et al.. Am. J, Obstet. Gynecol. March 1992, p. 962-968; Kamor et al..

Eng, J. of Med.. Nov 22, 1990, p. 1486-1487; DekkerstaL Eur J, Ob, and Gvn, and Rep. Bio. 40 (1991) 215-220; Schiff et al.. Am. J. Ostet. Gynecol. Feb 1992, p.

624-628; diabetes mellitus, Takahashi et al.. Diabetologia (1990) 33:306-310; and acute vascular rejection following kidney transplant, Watschinger et al.. Transplantation Vol. 52, No. 4, pp. 743-746.

Endothelin stimulates both bone resorption and anabolism and may have a role in the coupling of bone remodeling. Tatrai et al. Endocrinology. Vol.

131, p. 603-607.

Endothelin has been reported to stimulate the transport of sperm in the uterine cavity, Casey £LaL J. Clin, Endo and Metabolism. Vol. 74, No. 1, p. 223-225, therefore endothelin antagonists may be useful as male contraceptives. Endothelin modulates the ovarian/menstrual cycle, Kenegsberg, J. of Clin. Endo.

and Met.. Vol. 74, No. 1, p. 12, and may also play a role in the regulation of penile vascular tone in man, Lau £LaL Asia Pacific J. of Pharm.. 1991, 6:287-292 and Tejada etal.. J. Amer. Physio. Soc, 1991, H1078-H1085. Endothelin also mediates a potent contraction of human prostatic smooth muscle, Langenstroer et al..

J, Urology. Vol. 149, p. 495-499.

Thus, endothelin receptor antagonists would offer a unique approach toward die pharmacotherapy of hypertension, renal failure, ischemia induced renal failure, sepsis-endotoxin induced renal failure, prophylaxis and/or treatment of radio-contrast induced renal failure, acute and chronic cyclosporin induced renal failure, cerebrovascular disease, myocardial ischemia, angina, heart failure, asthma, atherosclerosis, Raynaud’s phenomenon, ulcers, sepsis, migraine, glaucoma, endotoxin shock, endotoxin induced multiple organ failure or disseminated intravascular coagulation, cyclosporin-induced renal failure and as an adjunct in angioplasty for prevention of restenosis, diabetes, preclampsia of pregnancy, bone remodeling, kidney transplant, male contraceptives, infertility and priaprism and benign prostatic hypertrophy.

SUMMARY OF THE INVENTION

This invention comprises indane and indene derivatives represented by Formula (I) and pharmaceutical compositions containing these compounds, and their use as endothelin receptor antagonists which are useful in the treatment of a variety of cardiovascular and renal diseases including but not limited to:

J.·

IC

O c

o

Cm

O>

-3hypertension, acute and chronic renal failure, cyclosporine induced nephrotoxicity, stroke, cerebrovascular vasospasm, myocardial ischemia, angina, heart failure, atherosclerosis, and as an adjunct in angioplasty for prevention of restenosis.

This invention further constitutes a method for antagonizing endothelin receptors in an animal, including humans, which comprises administering to an animal in need thereof an effective amount of a compound of Formula (I).

DETAILED DESCRIPTION OF THE INVENTION

The compounds of this invention are represented by structural

Formula (I):

wherein:

Ri is -X(CH₂)_nAr or -X(CH₂)_nR₈ or

AF/.'-7 9 4 / U 0 6 3 6

R₂ is hydrogen, Ar, C|_4alkyl, or (c);

Pps-XCCH^Rg;

P₂ is -X(CH₂)_nR₈, or -X-R9-Y;

R3 and R5 are independently hydrogen, Rj 1, OH,

Ci_₈alkoxy, S(O)qRn, N(Rg)₂, Br, F, I, Cl, CF3, NHCORg, R11CO2R7,

-X-R9-Y, or -X(CH₂)_nRg wherein each methylene group within

-X(CH₂)_nRg may be unsubstituted or substituted by one or two -(CH₂)_nAr groups;

-4AP . Ο Ο 573

R4 is hydrogen, R|j, OH, Cj^alkoxy, S(O)_qRi i,N(R(j)2, -X(Rn),

Br, F, I, Cl or NHCORg wherein the Ci^alkoxy may be unsubstituted or substituted by OH, methoxy or halogen;

R5 is independently hydrogen or Cj^alkyl;

R7 is independently hydrogen, Cp loalkyl, &l- jQalkenyl or

C2-8alkynyl all of which may be unsubstituted or substituted by one or more OH, N(R$)2, CO2R12» halogen or XCi^alkyl or R7 is (CH₂)_nAr;

Rg is hydrogen, Rj j, CO2R7, CO2C(Rj 1)2 O(CO)XR7, PO3<R7)₂, SO2NR7R1 j, NR7SO2R1 i,CONR7SO₂Ri μ SO3R7, SO2R7, P(O)(OR7)R7, CN,

-CO2(CH₂)_mC(O)N(R₆)_2> C^ i)₂N(R₇)₂, C(O)N(R₆)₂, tetrazole or OR₆;

R9 is (CH₂)n» divalent Cpigalkyl, divalent C₂-i()alkenyl or phenyl, ( all of which may be unsubstituted or substituted by one or more OH, N(R^)₂,

P COOH, halogen, or R9 may be )C=O or XC1 ^alkyl;

RjO is R3 or R4;

Rjl is hydrogen, Ar, Cj.galkyl, C₂-galkenyl, C2.galkynyl, all of which may be unsubstituted or substituted by one or more OH, CH2OH, N(Rg)2 or halogen;

Rl2 is hydrogen, Cj.^alkyl, C₂-6alkenyl or C2-7alkynyl;

X is (CH₂)_n, O, NRg or S(O)_q;

Y is CH3 or X(CH₂)_nAr;

Aris:

92900/4-6 ,'4/dV

naphthyl, indolyl, pyridyl, thienyl, oxazolidinyl, oxazolyl, thiazolyl, isothiazolyl, pyrazolyl, triazolyl, tetrazolyl, imidazolyl, imidazolidinyl, thiazolidinyl, isoxazolyl, oxadiazolyl, thiadiazolyl, morpholinyl, piperidinyl,

-510 c ²⁵

C piperazinyl, pyrrolyl, or pyrimidyl; all of which may be unsubstituted or substituted by one or more R3 or R4 groups;

A is C=O, or [C(R6)2]_m;

B is -CH2- or -O-;

Zj and Z2 are independently hydrogen, Ci.galkyl, C2-galkenyl, C2galkynyl, OH, Ci.galkoxy, S(O)qCi_galkyl, N(R6)2, Br, F, I, Cl, NHCORg, -X-R9-Y, -X(CH2)_nR8’ phenyl, benzyl or Cg.gcycloalkyl wherein the Ci.galkyl, C2.galkenyl or C2-galkynyl may be optionally substituted by COOH, OH, CO(CH2)nCH3, CO(CH2)_nCH2N(R(>)2, or halogen; or Zi and Z2 together may be -ΟΆ-Ο- on contiguous carbons;

Z3 is Zi or -X-R9-Y; q is zero, one or two; n is an integer from 0 to six; m is 1, 2 or 3;

and the dotted line indicates the optional presence of a double bond; or a pharmaceutically acceptable salt thereof; provided that

R2 is not hydrogen when X is S(O)q;

when the optional double bond is present there is only one R^q and there is no Pj and P2 is not NR^RqY;

when the optional double bond is present and X-R2 is attached to the double bond, X is not NR&

when the optional double bond is present and Ri is attached directly to the double bond, Ri is not NRgAR;

when R3, R5, Ζμ Z2, or Z3 is X(CH2)_nRs and n is not 0, X is oxygen or NR(j when Rg is OR$ or CO2H;

when Rg is [CO(CRj i)2O(CO)XR7] CC>2C(Ri i)2O(CO)XR7_j X is not S(O)_q;

the compound of Formula I is not (IRS)-1,3-diphenylindene-2carboxylic acid; (cis,cis)-l,3-diphenylindane-2-carboxylic acid; (1RS)3-[3-Methyl-l-phenyl-(lH)-ind-2-en-l-yl] propionic acid; or (1RS)2[l,3-diphenyl-(lH)-ind-2-en-2-yl]ethanoic acid; 1,3-diphenyl-lethoxyindene-2-carboxylic acid; 1,2,3-triphenylindene; 1,3 diphenylindene; 1-(2,3-dimethyl-2-buten-yl)-1,3-diphenylindene; 1,3diphenyl-2-methylindene; l,3-diphenyl-2-methylindane; 1,3diphenylindane; 5,6-dimethoxy-l,3-dimethoxyindene; l,3-bis(4,5dimethoxy-2-hydroxyphenyl)-5,6-dimethoxyindane; 1,3-bis(3,4dimethoxyphenyl)-5,6-dimethoxyindane; 1,3-diphenyl-235

-6AP.00573 methoxyidene, l,3-diphenyl-2-ethoxyindene, 5-fluoro-2-methylindene-3-acetic acid;

and further provided that compounds wherein:

Ri is -X(CH₂)_nAr or -X(CH₂)_nR8 or

R₂ is hydrogen, Ar or (c);

Pl is -X(CH₂)_nRg;

P₂ is -X(CH₂)_nRg, or -XR₉Y;

R3 and R5 are independently hydrogen, Rj μ OH, Ci.galkoxy,

S(O)_qRn, N(R₆)₂, Br, F, I, Cl, CF3, NHCORg, -XR9-Y or -X(CH₂)_nRg wherein die methylene groups of

-X(CH2)_nR8 unsubstituted or substituted by one or more -(CH₂)_nAr groups;

R4 is hydrogen, Ri μ OH, Ci^alkoxy, S(O)_qRi μ N(Rg)₂, -X(Ri 1),

Br, F, I, Cl or NHCORg wherein the

Ci.5alkoxy may be unsubstituted or substituted by OH, methoxy or halogen;

Rg is independently hydrogen or Cj^alkyl;

R7 is independently hydrogen, C1 .galkyl or (CH₂)_nAr,

Rg is hydrogen, Ri μ CO₂H, PO3H₂, P(O)(OH)R7 or tetrazole;

R9 is Ci.iffriicyi, C₂_iQalkenyl or phenyl all of which may be unsubstituted or substituted by one or more OH, N(Rg)₂, COOH, halogen or ΧΟμ ₅alkyl;

RiQisR3orR4;

Rl 1 is Ci.galkyl, C₂.galkenyl, C₂.galkynyl all of which may be unsubstituted or substituted by one or more OH, CH₂OH, N(Rg)₂ or halogen;

X is (CH₂)_n, O, NRg or S(O)_q;

Y is CH3 or -CH₂X(CH₂)_nAr, c

c

Ar is:

naphthyl, indolyl, pyridyl or thienyl, oxazolidinyl, oxazolyl, thiazolyl, isothiazolyl, pyrazolyl, triazolyl, tetrazolyl, imidazolyl, imidazolidinyl, thiazolidinyl, isoxazolyl, oxadiazolyl, thiadiazolyl, morpholinyl, piperidinyl, piperazinyl, pyrrolyl, ot pyrimidyl; all of which may be unsubstituted or substituted by one or more R3 or R4 groups;

A is C=O, or [C(R6)2]_m;

B is -CH2- or -O-;

Zj and Z2 are independently hydrogen,

Ci.galkyl, C2-8alkenyl, C2-8alkynyl, OH, Cj.galkoxy, S(O)qCi_8alkyl, N(R6>2. Br, F, I, Cl, NHC0R₆,

-X(CH2)nR8» phenyl, benzyl or C3_6cycloalkyl wherein the Cj.galkyl, C2-galkenyl or C2-8alkynyl may be optionally substituted by COOH, OH, CO(CH2)_nCH3, CO(CH2)_nCH2N(R6)2, or halogen; or Zj and Z2 together may be -O-A-O- on contiguous carbons;

Z3 is Zi or XR9Y; q is zero, one or two; n is an integer from 0 to six; m is 1,2 or 3; are excluded.

Also included in the invention are pharmaceutically acceptable salt complexes.

All alkyl, alkenyl, alkynyl and alkoxy groups may be straight or branched. The term halogen is used to mean iodo, fluoro, chloro or bromo.

Alkyl groups may be substituted by one or more halogens up to perhalogenation.

The compounds of the present invention may contain one or more asymmetric carbon atoms and may exist in racemic and optically active form. All of these compounds and diastereoisomers are contemplated to be within the scope

AP/P/ 9 4/ 0 0 6 3 6

-8AP.00573 of the present invention.

Preferred compounds are those wherein Rj is X(CH2)_nAr, (Ar is (a) or (b)), dihydrobenzofuranyl, benzodioxanyl, cyclohexyl, Ci.4alkyl; R2 is (a), (b) Ci_4alkyl, indolyl or hydrogen; R3 and R5 are independently hydrogen, OH,

C μ 5 alkoxy, halogen, -OCj^alkyl phenyl, Ri iC02R_7j Cj^alkyl, N(Rg)2,

NH(COXH3_t -X(CH2)_nR8, -XR9 pyridyl, phenyl or S(O)pCj.5alkyl; R4 is hydrogen, OH, C^alkoxy, halogen, Cj^alkyl, N(Rg)2, NH(CO)CH3 or S(O)pCi_5alkyl; Ζμ Z2 and Z3 are independently XR9Y, benzyl, hydrogen, OH, C^alkoxy, -N(R(>)2, S(O)qCi_galkyl, NHCOR5, X(CH2)_nRg or halogen, or Zj and Z2 together may be -O-A-O on contiguous carbons; Pi and P2 are independently hydrogen, CO2H or tetrazole; Ar is (a), (b), phenyl, or pyridyl; X is (CH2)_n or oxygen.

More preferred are compounds wherein R3 is hydrogen or X(CH2)_nRg, Rl1CO2R7; R4 and R5 are independently hydrogen, OH,

Ci_5alkoxy, SCj^alkyl, F, Br, Cj^alkyl or ΝΗ2; Zj and Z3 are hydrogen and Z2 is hydrogen, OH, Ci_5alkoxy, halogen, X(CH2>_nRg, ΝΗ2, benzyl, NH(CO)CH3, <w Zj and Z2 together may be O-A-O.

Most preferred are compounds wherein Rj is (b) and R2 is (a) or (b); A is CH2, B is -O-; there is no optional double bond; Ri and XR2 are trans to

Pj; Z2 is OH, Ci_5alkoxy, -OCH2CHCH2 or hydrogen, Zi is hydrogen; R3 is XAr, hydrogen, X(CH2)_qCOOH, X(CH2)_qCONR₇SO2Ri 1 or CH=CHCO2H, R₄ is hydrogen, substituted phenyl, or Ci_2alkoxy; and R5, Rjq and P2 are hydrogen.

Also included are the following compounds:

(+) (1 S,2R,3S)-3-(2-Carboxymethoxy-4-methoxyphenyl)-1 -(3,4methylenedioxyphenyl)-5-(prop-1 -yloxy )indane-2-carboxylic acid;

AP/P/ 9 4 / U 0 6 3 6 (IRS, 2SR, 3RS)-3-[2-[(4-Carboxypyridin-3-yl)oxy]-4- methoxyphenyl]-1-(3,430 methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylic acid disodium salt;

(+) (IS, 2R, 3S)-3-[2-(2-Hydroxyeth-l-yloxy)-4-methoxyphenyl]-1-(3,4methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-2-carboxylic acid, dicyclohexylamine salt;

-9(IS, 2R, 3S)-3-[(2,2-dimethylpropanoyloxymethoxycarbonylmethoxy)-4-methoxyphenyl]-1-(3,4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylic acid sodium salt.

The present invention provides compounds of Formula (I) above

which can be prepared by a process which comprises:

a) reacting a compound of Formula (2) wherein X is Chalky!

Zi o

CO₂X *3 (2) with a substituted benzaldehyde or aldehyde of Formula (3).

D-CHO (3)

- 10AP . Ο Ο 5 7 3 wherein D is Ar or (c) as defined in Formula I, in a suitable solvent such as benzene with a catalyst such as piperidinium acetate at reflux to provide a compound of Formula (4).

(4)

Cyclization of compound (4) in the presence of a suitable Lewis acid such as titanium tetracholoride or aluminum chloride or alternatively when Zj is 3OR (meta)(where R is C^alkyl, or benzyl), trifluoroacetic acid, provides an indanone of the Formula (5).

(5)

Dehydrogenation with 2,3-dichloro-5,6-dicyano-l,4-benzoquinone in an appropriate solvent or alternatively bromination with pyridinium hydrobromide perbromide in dichloromethane followed by treatment with 1,5diazabicyclo[4,3,0]non-5-ene provides indenones of Formula (6).

tC

C c

o c

o

CO₂X (6)

- 11 b) Alternatively, a compound of Formula 6 wherein Ζμ Z2 and Z3 are hydrogen and

can be prepared by treatment of 2-bromobenzoic acid with two equivalents of nbutyllithium in a solvent such as tetrahydrofuran under argon at -78°C followed by the addition of an acid chloride of Formula (7):

Treatment of compounds of type (8) with thionyl chloride at reflux gives an acid chloride which can be isolated by concentration under reduced

- 12AP.00573 pressure. This acid chloride can then be treated with diethyl magnesium malonate in a solvent such as ether to give a compound of Formula (9):

On *> -η

Reaction of a compound of type (9) at reflux with 5% aqueous sodium carbonate gives compounds of Formula (10):

c) Treatment of an indenone of Formula (11):

CO₂X

I 'Lt' «.j (10)

-t* o

α>

(11)

wherein Ζμ Ζ2, Ζ3 and R} are as defined for formula I or a group convertable to them, with an organomagnesium compound of Formula (12) wherein R2 is defined for

R₂(CH₂)_nMgBr (12)

Formula I or a group convertable to it, in a suitable solvent provides compounds of Formula (13):

(13)

Saponification of compounds of Formula (13) using sodium hydroxide in 15 aqueous methanol followed by reduction with triethylsilane and boron trifluoride etherate in a suitable solvent such as dichloromethane at 0°C affords racemic compounds of Formula (14).

(14)

Conjugate addition of nucleophiles to an ester derived from Formula (14), followed by saponification affords compounds of Formula (I) having an Rjq other than hydrogen. Re-introduction of a double bond into an ester derived from such acids followed by conjugate addition of another nucleophilic species and subsequent saponification affords compounds of Formula (1) in which neither Rjq substituent is hydrogen.

Reduction of compounds of Formula (13) with triethylsilane and boron trifluoride etherate in a suitable solvent such as dichloromethane at 0°C followed by hydrogenation with hydrogen gas under pressure at approximately 60

- 14AP. Ο Ο 5 7 3 psi in the presence of a suitable catalyst such as 10% palladium on charcoal affords

(15)

Alkylation or acylation of the ester enolate derived from Formula (15) affords compounds wherein Pj and P2 are as defined in Formula (1).

Alternatively, hydrogenation of compounds of Formula (13) with hydrogen gas under pressure at approximately 60 psi in the presence of a suitable catalyst such as 10% palladium on charcoal in a suitable solvent such as ethyl acetate or methanol containing 1-5% acetic acid affords compounds of Formula (15) . Treatment of these compounds with a base such as sodium hydroxide in a suitable solvent such as aqueous ethanol provides racemic compounds of Formula (16) :

Mr

Ο

Ο 20

(16) wherein Ζμ Z2 and Z3 are hydrogen; Ri = R2; and n is 0.

Treatment of compounds of Formula (13) with triethylsilane and boron trifluoride etherate in a suitable solvent such as dichloromethane at 0°C followed by reaction with samarium Π iodide in a suitable solvent such as tetrahydrofuran and then saponification, provides compounds of Formula (17)

u.

-C c

c σ

Cz cr.

(17)

- 1510

C ²⁵ c

With appropriate manipulation and protection of any chemical functionalities, synthesis of the remaining compounds of the Formula (I) is accomplished by methods analogous to those above and to those described in the

Experimental section.

In order to use a compound of the Formula (I) or a pharmaceutically acceptable salt thereof for the treatment of humans and other mammals it is normally formulated in accordance with standard pharmaceutical practice as a pharmaceutical composition.

Compounds of Formula (I) and their pharmaceutically acceptable salts may be administered in a standard manner for the treatment of the indicated diseases, for example orally, parenterally, sub-lingually, transdermally, rectally, via inhalation or via buccal administration.

Compounds of Formula (I) and their pharmaceutically acceptable salts which are active when given orally can be formulated as syrups, tablets, capsules and lozenges. A syrup formulation will generally consist of a suspension or solution of the compound or salt in a liquid carrier for example, ethanol, peanut oil, olive oil, glycerine or water with a flavouring or colouring agent. Where the composition is in the form of a tablet, any pharmaceutical carrier routinely used for preparing solid formulations may be used. Examples of such carriers include magnesium stearate, terra alba, talc, gelatin, agar, pectin, acacia, stearic acid, starch, lactose and sucrose. Where the composition is in the form of a capsule, any routine encapsulation is suitable, for example using the aforementioned carriers in a hard gelatin capsule shell. Where the composition is in the form of a soft gelatin shell capsule any pharmaceutical carrier routinely used for preparing dispersions or suspensions may be considered, for example aqueous gums, celluloses, silicates or oils and are incorporated in a soft gelatin capsule shell.

Typical parenteral compositions consist of a solution or suspension of the compound or salt in a sterile aqueous or non-aqueous carrier optionally containing a parenterally acceptable oil, for example polyethylene glycol, polyvinylpyrrolidone, lecithin, arachis oil, or sesame oil.

Typical compositions for inhalation are in the fonn of a solution, suspension or emulsion that may be administered as a dry powder or in the form of an aerosol using a conventional propellant such as dichlorodifluoromethane or trichlorofluoromethane.

- 1610

ΑΡ.00573 c ²⁵ ο

A typical suppository formulation comprises a compound of Formula (1) or a pharmaceutically acceptable salt thereof which is active when administered in this way, with a binding and/or lubricating agent, for example polymeric glycols, gelatins, cocoa-butter or other low melting vegetable waxes or fats ar their synthetic analogues.

Typical transdermal formulations comprise a conventional aqueous or non-aqueous vehicle, for example a cream, ointment, lotion or paste or are in the form of a medicated plaster, patch or membrane.

Preferably the composition is in unit dosage form, for example a tablet, capsule or metered aerosol dose, so that the patient may administer to themselves a single dose.

Each dosage unit for oral administration contains suitably from 0.1 mg to 500 mg/Kg, and preferably from 1 mg to 100 mg/Kg, and each dosage unit for parenteral administration contains suitably from 0.1 mg to 100 mg, of a compound of Formula (I) or a pharmaceutically acceptable salt thereof calculated as the free acid. Each dosage unit for intranasal administration contains suitably 1400 mg and preferably 10 to 200 mg per person. A topical formulation contains J suitably 0.01 to 1.0% of a compound of Formula (I). J ·» The daily dosage regimen for oral administration is suitably about 0.01 mg/Kg to 40 mg/Kg, of a compound of Formula (I) or a pharmaceutically <x acceptable salt thereof calculated as the free acid. The daily dosage regimen for ·®* parenteral administration is suitably about 0.001 mg/Kg to 40 mg/Kg, of a compound of the Formula (I) or a pharmaceutically acceptable salt thereof _c calculated as the free acid. The daily dosage regimen for intranasal administration cn and oral inhalation is suitably about 10 to about 500 mg/person. The active ingredient may be administered from 1 to 6 times a day, sufficient to exhibit the desired activity.

No unacceptable toxicological effects are expected when compounds of the invention are administered in accordance with the present invention.

Hie biological activity of the compounds of Formula (I) are demonstrated by the following tests:

I. Binding Assay

A) Membrane Preparation

Rat cerebellum or kidney cortex were rapidly dissected and frozen immediately in liquid nitrogen or used fresh. The tissues, 1-2 g for cerebellum or 3-5 g for kidney cortex, were homogenized in 15 mis of buffer containing 20mM

- 1710 c ²⁵ (

Tris HCI and 5mM EDTA, pH 7.5 at 4°C using a motor-driven homogenizer. The homogenates were filtered through cheesecloth and centrifuged at 20,000 x g for 10 minutes at 4°C. The supernatant was removed and centrifuged at 40,000 xg for 30 minutes at 4°C. The resulting pellet was resuspended in a small volume of buffer containing 50 mM Tris, 10 mM MgCl2, pH 7.5; aliquotted with small vials and frozen in liquid nitrogen. The membranes were diluted to give 1 and 5 mg of protein for each tube for cerebellum and kidney cortex in the binding assay.

Freshly isolated rat mesenteric artery and collateral vascular bed were washed in ice cold saline (on ice) and lymph nodes were removed from along the major vessel. Then, the tissue was homogenized using a polytron in buffer containing 20 mM Tris and 5mM EDTA, pH 7.5 at 4°C in 15 ml volume for ~6 gm of mesenteric artery bed. The homogenate was strained through cheesecloth and centrifuged at 2,000 xg for 10 min. at 4°C. The supernatant was removed and centrifuged at 40,000 xg for 30 min. at 4°C. The resulting pellet was resuspended as explained above for cerebellum and kidney cortex. Approximately 10 mg of membrane protein was used for each tube in binding experiments.

B) Γ^ΠΕΤ-! Binding Protocol [125ΐ]Εψ_ι binding to membranes from rat cerebellum (2-5 mg protein/assay tube) or kidney cortex (3-8 mg protein/assay tube) were measured after 60 minutes incubation at 30°C in 50 mM Tris HCI, 10 mM MgCl2,0.05% BSA, pH 7.5 buffer in a total volume of 100 ml. Membrane protein was added to tubes containing either buffer or indicated concentration of compounds.

(2200 Ci/mmol) was diluted in the same buffer containing BSA to give a final concentration of 0.2-0.5 nM ET-1. Total and nonspecific binding were measured in the absence and presence of 100 nM unlabelled ET-1. After the incubation, the reactions were stopped with 3.0 ml cold buffer containing 50 mM Tris and 10 mM MgCl2, pH 7.5. Membrane bound radioactivity was separated from free ligand by filtering through Whatman GF/C filter paper and washing the filters 5 times with 3 ml of cold buffer using a Brandel cell harvester. Filter papers were counted in a gamma counter with an efficiency of 75%. IC50S for the compounds of this invention range from 0.1 nm to 50 mm.

Π. In Vitro Vascular Smooth Muscle Activity

Rat aorta are cleaned of connective tissue and adherent fat, and cut into ring segments approximately 3 to 4 mm in length. Vascular rings are suspended in organ bath chambers (10 ml) containing Krebs-bicarbonate solution of the following composition (millimolar): NaCl, 112.0; KC1, 4.7; KH2PO4, 1.2;

>

z·

C

σ.

oo>

- 18AP . Ο Ο 5 7 3 ζ·'· 25 c

MgSC>4,1-2; CaCl₂, 2.5; NaHC03, 25.0; and dextrose, 11.0. Tissue bath solutions are maintained at 37°C and aerated continuously with 95% 0₂/ 5% CO₂. Resting tensions of aorta are maintained at 1 g and allowed to equilibrate for 2 hrs., during which time the bathing solution is changed every 15 to 20 min. Isometric tensions are recorded on Beckman R-611 dynographs with Grass FT03 force-displacement transducer. Cumulative concentration-response curves to ET-1 or other contractile agonists are constructed by the method of step-wise addition of the agonist. ET-1 concentrations are increased only after the previous concentration produces a steady-state contractile response. Only one concentration-response curve to ET-1 is generated in each tissue. ET receptor antagonists are added to paired tissues 30 min prior to the initiation of the concentration-response to contractile agonists.

ET-1 induced vascular contractions are expressed as a percentage of the response elicited by 60 mM KC1 for each individual tissue which is determined at the beginning of each experiment. Data are expressed as the mean ± S.E.M.

Dissociation constants (K5) of competitive antagonists were determined by the standard method of Arunlakshana and Schild. The potency range for compounds of this invention range from 0.1 nM to 50 mm. *

The following examples are illustrative and are not limiting of the compounds of this invention.

u

EXAMPLE 1 ί ύύ (lS.2R.3S)-3-(2-Carboxymethoxy-4-methoxvohenyl)-1 -(3.4- _c methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-2-carboxvlic acid e

a) 3-(Prop-1 -yloxy)acetophenone. To a slurry of NaH (13.84 g, 0.58 mol) in dry

DMF (50 mL) at 0°C, was added a solution of 3-hydroxyacetophenone (50 g, 0.37 mol). After stirring for 30 min. 1-iodopropane (70 mL, 0.72 mol) was added and the mixture stirred overnight at room temperature. The mixture was diluted with dry DMF (50 mL) and further NaH (2.77 g, 0.12 mol) added followed by 1iodopropane (23 mL, 0.24 mol). After 1 h. TLC indicated that the reaction was complete and the product was cautiously quenched with 6M HCI and extracted with

EtOAc. The EtOAc extract was washed successively with Η₂0,10% aqueous

NaOH and then brine. After drying (MgSO4), filtration and evaporation gave the title compound (65 g, 98%) as a light yellow oil which was used without further purification. Anal. Calc, for CuHi4O₂: C, 74.13; H, 7.89. Found: C, 73.85;

H, 7.86.

- 19b) Methyl S-fProp-l-yloxylbenzovlacetate. To a suspension of NaH (12 g, 0.5 mol) in dry dimethyl carbonate (50 mL) was added slowly a solution of 3-(Prop-lyloxy)acetophenone (65 g, 0.37 mol) in dry dimethyl carbonate (100 mL). During the addition the exothermicity of the reaction caused refluxing. Following the addition the mixture was stirred mechanically overnight and was then quenched cautiously with 3M HCI and extracted with EtOAc. The EtOAc extract was washed successively with H2O, 5% aqueous NaHCO3, H2O and brine. After drying (MgSO4), filtration and evaporation gave a yellow oil (82 g, quantitative) which was used without further purification.

c) Methyl 3-(3.4-methylenedioxyphenvP-2-f3-(prop-1-vloxy)benzoyllpropenoate.To a solution of methyl 3-(prop-l-yloxy)benzoylacetate (10 g,

4.2 mmol) in benzene (50 mL) was added 3,4-methylenedioxybenzaldehyde (6.36 g, 4.2 mmol) followed by piperidine (0.42 mL, 0.42 mmol) and glacial acetic acid (8 drops approx.). The mixture was refluxed for 2 h. and the volatiles removed in vacuo to give methyl

3-(3,4-methylenedioxyphenyl)-2-[3-(prop- l-yloxy)-benzoyl]propenoate (7.4 g, 48%) as an off white solid after trituration with methanol (m. p. 122-123°C). Anal. Calc, for C₂i H20O6: C, 68.47; H, 5.47. Found: C, 68.81; H, 5.49.

d) Methyl (lRS.2SR)-l-(3.4-Methylenedioxyphenyl)-5-(DroD-l-yloxy)-3-oxoindane-2-carboxylate. Methyl 3-(3,4-methylenedioxyphenyl)-2-[3-(prop-l-yloxy)benzoyljpropenoate (7.4 g, 2.0 mmol) was dissolved in trifluoroacetic acid (50 mL) at 0°C and the mixture stirred at room temperature for 20 min. The trifluoroacetic acid was removed in vacuo to give the title compound (6.4 g, 87 %) as a white solid after trituration with warm isopropanol m. p. 106-108°C. Anal. Calc, for C21 Η20θ6^: C, 68.47; H, 5.47. Found: C, 68.12; H,5.41.

e) Methyl 3-(3.4-Methylenedioxyphenyl)-6-(prop-l-yloxy)-l-oxo-indene-230 carboxylate. Methyl (IRS, 2SR)-1-(3,4-methylenedioxyphenyl)-5-(prop-l-yloxy)3-oxo-indane-2-carboxylate (26.2 g, 71 mmol) was dissolved in toluene (250 mL) and DDQ (dichlorodicyano-quinone) (16.5 g, 71 mmol) was added. The mixture was heated at 80°C for 2 h. then cooled, filtered and the solvent removed in vacuo. The product was purified by flash column chromatography on silica gel (eluant:

EtOAc/hexane, 20:80) to give the title compound as an orange solid (11.3 g, 44 %);

m.p. 125-126°C. Anal. Calc, for C21 HisOg: C, 68.85; H, 4.95. Found: C, 68.45; H, 4.97.

- 20AP.0 0 57 J

f) Methvl (lRS~)-l-Hvdroxv-l-(4-methoxv-2-methoxvmethoxvphenvn-3-(3.4methylenedioxyphenvl)-6-fprop-l-yloxv)indene-2-carboxvlate. To dry magnesium turnings ¢1.7 g, 69 mmol) under an argon atmosphere was added portionwise, a 5 solution of l-bromo-4-methoxy-2-methoxymethoxybenzene (16.8 g, 68 mmol) in 5% THF/ether (120 mL). The resulting 4-methoxy-2-methoxymethoxyphenyl magnesium bromide was added to a solution of methyl 3-(3,4methylenedioxyphenyl)-6-(prop-l-yloxy)-l-oxo-indene-2-carboxylate (18.5 g, 51 mmol) in THF (400 mL) under an argon atmosphere at O°C. The resulting mixture 10 was allowed to warm to room temperature and was stirred for 10 min. The mixture was partitioned between 3M HCI and EtOAc. The organic extract was washed successively with H2O, aqueous NaHCO3, H2O and saturated aqueous NaCl and dried (Na2SO4). The solvent was removed under reduced pressure, and the residue purified by flash chromatography on silica gel (eluant: EtOAc/hexane, 10-20%) to 15 afford the title compound as a yellow oil (24.5 g. 91%). Anal. Calc, for C30H30O9: C, 67.41; H, 5.66;. Found: C, 67.21; H, 5.66.

SEPARATION

Separation of (+) and (-) methyl (lRS)-l-Hydroxy-l-(4-methoxy-2methoxymethoxyphenyl)-3-(3,4-methylenedioxyphenyl)-6-(prop-l-yloxy)indenc-2carboxylate was done on a column of cellulose tris(3,5-dimethylphenyl carbamate) coated on silica gel (Daicel Chiralcel OD); retention time for,(+) 8.8 min. [α]25θ+87.5° (c = 0.24, CH3OH). Retention time for (-) 14.5 min. [a]25p= +85.9° (c = p 25 0.21.CH3OH)

HPLC data; column Chiralcel OD (DAICEL) 21.2 mm internal diameter, 250 mm length; solvent Ethanol:Hexane 60:40; flow rate 10 mL/min.; injection: 1 g of racemate; detection UV = 405 nm

g) (+)Methyl(lS.2S.3Sl-3-(4-Methoxy-2-methoxymethoxyDhenyn-l-(3.4mcthykncdiQxyphsnyl)^.-IprQp-1 -ylQxy)indang-2-partx>*ylatg, a parr vessel was charged with (+) methyl (lRS)-l-Hydroxy-l-(4-methoxy-2methoxymethoxyphenyl)-3-(3,4-methylenedioxyphenyl)-6-(prop-l-yloxy)indene-2carboxylate (1 g, 1.8 mmol) dissolved in a small volume of EtOAc (25 mL) and

10% palladium on activated carbon (93 mg). The resulting solution was stirred under an atmosphere of hydrogen for 120 hours and filtered. The filtrate was concentrated under reduced pressure and the product purified by column

AP/P/ 94/00636

-21 chromatography on silica gel (eluant: EtOAc/hexane, 5-10%) to give the title compound as a white foam (0.80 g, 83%). [a]²5_D= +105.4° (c = 0.13, CH3OH).

Anal. Calc, for C30H32O8: C, 69.22; H, 6.20. Found: C, 68.95; H, 6.11.

h) f+)Methvl (lS.2S.3S)-3-(2-Hvdroxy-4-methoxyphenyl)-1-(3.4methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-2-carboxylate. To a solution of methyl (lS,2S,3S)-3-(4-Methoxy-2-methoxymethoxyphenyl)-1-(3,4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylate (0.7 g, 1.3 mmol) in methanol (10 mL) concentrated HCI (0.1 mL) was added and it was then heated to reflux for 2 h. The solvent was then eliminated under vacuum and the residue was purified by column chromatography on silica gel (eluant: EtOAc/hexane, 10-20%) to give the title compound as a colorless glass (0.50 g, 78%). [α]²^θ= +116.0° (c = 0.18, CH3OH). Anal. Calc, for C28H28O7.I/2 H₂O: C, 69.27; H, 6.02.

' Found: C, 69.59; H, 5.99.

i) i±l Methyl (1S.2S.3S)-3-(2-Carboethoxymethoxy-4-methoxvphenyl)-l-(3.4methvlenedioxvphenvl)-5-(prop-1 -vloxv)indane-2-carboxvlate. A solution of methyl (lS,2S,3S)-3-(2-hydroxy-4-methoxyphenyl)-l-(3,4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylate (0.1 g, 0.2 mmol) in dry DMF (2 mL) was added to NaH (6 mg, 0.24 mmol) in a small volume of dry DMF at 0°C. The mixture was stirred at 0°C for 15 min. and ethyl bromoacetate was then added (42 mg, 0.25 mmol). The resulting mixture was stirred at 0°C for lh. The reaction was then quenched with dilute HCI and extracted with EtOAc.

The EtOAc extract was washed with water then brine, dried (MgSO4), filtered and ¢-, 25 evaporated. The product was purified by column chromatography on silica gel ,- (eluant: EtOAc/hexane, 10-15%) to give the title compound as a glassy solid (82 mg, 68%).[a]²⁵D= +116.0° (c = 0.45, CH3OH). Anal. Calc, for C32 H34O9: C, 68.32; H, 6.09. Found: C, 67.98; H, 6.09.

j) (+) (1 S.2R.3S)-3-(2-Carboxymethoxy-4-methoxyphenyl)-1 -(3.4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylic acid. To a solution of methyl (lS,2S,3S)-3-(2-carboethoxymethoxy-4-methoxyphenyl)-1-(3,4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylate (20 mg, 0.04 mmol) in dioxane (1 mL) was added 3 M NaOH solution (0.3 mL, 1 mmol). The reaction mixture was heated to reflux for 4 h and after cooling the solvent was eliminated in vacuo dissolved in water and acidified with 3N HCI. The resulting precipitate was __collected by filtration and dried to give a white solid (15 mg, 81%); m.p. 99-102°C

AP/P! 94/00636

- 22 AP.00573 [α]²⁵0= +38.1° (c = 0.22, CH3OH). Anal. Calc, for C29H28 O9: C, 66.92; H, 5.42. Found C, 67.37; H, 5.32.

EXAMPLE IA

Preparation of l-Bromo-4-methoxy-2-methoxymethoxvbenzene,

a) l-Bromo-2-hydroxy-4-methoxybenzene. 3-Bromo-2-hydroxy-6methoxybenzoic acid [T. de Paulis et. al. .J, Med. Chem.. (1985), 28, 1263-1269] (5 g, 0.02 mol) was heated in quinoline (200 mL) at 160°C for 1 h. On cooling, the product was partitioned between Et2O and 3M HCI. The organic extract was washed with water and brine then dried (MgSO4), filtered and evaporated to give the title compound which was recrystallized from 5% ethyl acetate/hexane (4 g, 97%); m.p. 40-42°C. Anal. Calc, for C7H7 BrO2: C, 41.41; H, 3.48.

Found C, 41.39; H, 3.37.

b) l-Bromo-4-methoxy-2-methoxymethoxybenzene, To a suspension of NaH (2.5 g, 0.06 mol) in dry DMF (100 mL) at 0°C was added solution of l-bromo-2hydroxy-4-methoxybenzene (10.6 g, 0.05 mol). After stirring at 0°C for 30 min. bromomethyl methyl ether (7.8 g, 0.06 mmol) were dropwise added. The mixture was warmed to room temperature over 20 min. and then stirred for 2 h, it was then quenched cautiously by the addition of cold dilute HCI and extracted with EtOAc. The EtOAc extract was washed successively with; H20,5% aqueous NaHCO3, H2O and finally brine. After drying (MgSO4) filtration and evaporation gave liquid. The product was purified by distillation (85°C, 0.2 mm Hg) to give the title compound as a colorless oil (13.7 g, 97%).

NMR (CDCI3) δ 7.40 (d, 1 H, J = 8.9 Hz), 6.75 (d, 1 H, J = 2.8 Hz), 6.46 (dd,

H, J = 8.9,2.8 Hz), 5.23 (s, 2 H), 3. 77 (s, 3 H), 3.52 (s, 3 H).

Cz σ

EXAMPLE 2 (1RS.2SR. 3RS)-3-r2-(2-Hydroxyeth-l-yloxy)-4-methoxyphenyll-l-(3.4methylenedioxyphenvl)-5-f prop-1 -yloxy)indane-2-carboxylic acid.

dicyclohexyiamine salt

m.p. 182-184°C.

Anal. Calc, for C41H53NO8: C, 71.59; H, 7.77; N, 2.04. Found: C, 71.67; H, 7.66; N, 2.42.

-23EXAMPLE 2A

M (IS. 2R. 3S)-3-i2-(2-Hydroxyeth-l-vloxy)-4-methoxyphenyll-l-(3.4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxyIic acid

a) f+) Methvl-dS. 2R. 3S)-3-f2-(2-r-ButyldimethylsiIoxveth-l-yloxy)-4methoxyphenyll-l-(3.4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2carboxylate. A solution of methyl-(lS,2S,3S)-3-(2-hydroxy-4-methoxyphenyl)-l(3,4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylate (3 g, 6 mmol) in dry DMF (30 mL) was added to NaH (230 mg, 7 mmol, 80%) in a small volume of dry DMF at 0°C. The mixture was stirred at 0°C for 15 min. and 2-fbutyldimethylsiloxyethylbromide was then added dropwise (1.65 g, 8 mmol). The resulting mixture was stirred at 0°C for 3 h. The reaction was then quenched with dilute HCI and extracted with EtOAc. The EtOAc extract was washed with water then brine, dried (MgSO4), filtered and evaporated. The product was purified by column chromatography on silica gel (eluant: EtOAc/hexane, 5-10%) to give the title compound as a colorless oil (520 mg, 18% based on recovery of epimerized starting material).

b) Methyl-(+) (IS. 2R. 3S)-3-f2-(2-Hvdroxyeth-l-yloxy)-4-methoxyphenyll-l20 (3.4-methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-2-carboxylate. To a solution of (+)methyl-(lS, 2R, 3S)-3-[2-(2-f-butyldimethylsiloxyeth-l-yloxy)-4methoxyphenyl]-1-(3,4-methylenedioxyphenyl)-5-(prop-1-yloxy)indane-2carboxylate (0.520 g, 0.74 mmol) in THF (10 mL) at RT, a 1 M solution of tetra-nbutylammonium fluoride (2.5 mL) in THF was added. The reaction mixture was stirred at RT for 1 h. After concentration in vacuo, the residue was partitioned between ether/ethyl acetate and water. The organic layer was washed with brine, dried (MgSO4) and concentrated. The residue was purified by flash column chromatography on silica gel (eluant: 20-30% ethyl acetate/hexane) to give the title compound as a colorless foam (0.35 g, 82%).

c) (+) (IS. 2R, 3S)-3-f2-(2-Hydroxyeth-l-yloxy)-4-methoxyphenyn-l-(3.4methylenedioxyphenvl)-5-(prop-l-yloxv)indane-2-carbQxvlic_agid

To a solution of methyl-(lS,2R,3S)-3-[2-(2-hydroxyeth-l-yloxy)-4methoxyphenyl)-1-(3,4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-235 carboxylate (0.35 g, 0.67 mmol) in methanol/THF 1/2(15 mL) was added a 0.5 M LiOH solution (5 mL). The reaction mixture was stirred at RT overnight. The solvent was eliminated in vacuo the product dissolved in water and acidified with

- 24AP.00573

3N HCl. The resulting precipitate was collected by filtration and dried to give a white solid (0.31 mg, 91%); m.p. 94-98°C.[a]²³D= +73.16° (c = 0.19, CHjOH) Anal. Calc, for C29H290gNa.l 1/8 H2O: C, 63.47; H, 5.74. Found: C, 63.39; H, 5.66.

EXAMPLE 3 (IRS. 2SR. 3RS)-3-f2-i(4-Carboxypyridin-3-vI)oxv1-4methoxyphenyn-l-(3.4-methylenedioxyphenyl)-5-(prop-l--yloxv)indane-2carboxylic acid disodium salt;

a) Methyl (IRS. 2SR. 3RS)-3-i2-r(4-Formylpyridin-3-ynoxyl-4-methoxyphenyl1l-(3.4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylate

C To a solution of Methyl (IRS, 2SR, 3RS)-3-(2-hydroxy-4-methoxyphenyl)-1-(3,415 methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylate (300 mg, 0.63 mmol) in DMF (4 mL) was added K2CO3 (109 mg, 0.79 mmol) and 3-fluoro-4fonnylpyridine (150 mg, 1.2 mmol). The reaction mixture was heated to reflux under argon for 2 h. After cooling to room temperature it was partitioned between 3N HCl and ethyl acetate. The ethyl acetate extract was washed with water, aqueous NaHCO3 and brine and dried (MgSO4). The solvent was removed in vacuo and the residue purified by flash column chromatography (silica gel, gradient elution from 10% to 20% ethyl acetate/hexanes) to afford the title compound (128 mg, 42%).

¢- 25 b) Methyl (IRS. 2SR, 3R5.L2-J22j.(4-CarbQ^ypyridin-3-yl)oxy]-4Q methoxyphenyIl-l-(3.4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2carboxylatc

To a solution of Methyl (IRS, 2SR, 3RS)-3-[2-[(4-formylpyridin-3-yl)oxy]-430 methoxyphenyl]-1-(3,4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2carboxylate (128 mg, 0.24 mmol) in t-BuOH (10 mL) was added a solution of NaQO2 (34 mg, 0.28 mmol) and NH2SO3H (40 mg, 0.42 mmol) in water (6mL). The reaction mixture was stirred at room temperature for 2 h and partitioned between water and ethyl acetate. The ethyl acetate extract was washed with water and brine and dried (MgSO4). The solvent was removed in vacuo the residue was purified by flash column chromatography (silica gel, 25% ethyl acetate/hexanes containing 5% of acetic acid) to afford the title compound (90 mg, 69%).

-2510 <

r ²⁵ ί

c) (IRS. 2SR. 3RS)-3-i2-f(4-Carboxypyridin-3-yl)oxvl-4-methoxyphenyll-l-(3.4methvlenedioxvphenvl)-5-(prop-l-vIoxy)indane-2-carboxvlic acid disodium salt

To a solution of Methyl (IRS, 2SR, 3RS)-3-[2-[(4-Carboxypyridin-3-yl)oxy]-4methoxyphenyl]-l-(3,4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2carboxylate (90, 0.15 mmol) in isopropanol (2 mL) was added 1M aqueous NaOH (0.3 mL, 0.3 mmol). The resulting mixture was heated to reflux for 12 h, then concentrated under reduced pressure. The residue was partitioned between dilute HCI and ethyl acetate. The ethyl acetate extract was washed with water and dried (MgSO4>. The solvent was removed in vacuo and the residue was purified by flash column chromatography (silica gel, 30% ethyl acetate/hexanes containing 5% of acetic acid) to afford the title compound (65 mg, 74%); m.p. 220-222°C (dec.) (disodium salt).

EXAMPLE 4

2.2-DimethvlDrooanovloxvmethvl (lRS.2SR.3RS)-3-(2-carboxymethoxy-4methoxyphenyl)-l-(3.4-methylenedioxyphenyI)-5-(prop-l-yloxy)indane-2carboxylate sodium salt

a) 2-2-Dimethylpropanoyloxymethyl (lRS.2SR.3RS)-3-C2-hydroxy-4methoxyphenyl)-l-(3.4-methylenedioxyphenyB-5-(prop-l-yloxy)indane-2carboxvlate (lRS,2SR,3RS)-3-(2-hydroxy-4-methoxyphenyl)-1-(3,4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylic acid potassium salt (125 mg, 0.54 mmol) (obtained by treatment of the corresponding acid with KHCO3 (54 mg, 0.54 mmol)) was dissolved in DMF (3 ml) and pivaloyloxymethyl iodide (0.54 mmol) (prepared from pivaloyloxymethyl chloride (73 mg, 0.54 mmol) and excess sodium iodide in acetone) added. The reaction mixture was stirred overnight and then partitioned between dil. HCI and ethyl acetate. The organic layer was washed with water and brine then dried (MgSO4 anhyd.) filtered and evaporated. The product was purified by column chromatography to provide the title compound (120 mg, 77%) as a colorless oil.

b) 2.2-Dimethylpropanoyloxymethyl (IRS. 2SR. 3RS)-3-(2carbobenzyloxymethoxy-4-methoxyphenyl)-l-(3.4-methylenedioxyphenyl)-5(prop-1 -yloxy)indane-2-carboxylate.

2,2-Dimethylpropanoyloxymethyl (lRS,2SR,3RS)-3-(2-hydroxyti

-Cc σ

o cr.

-26AP. Ο Ο 5 7 3

4-methoxyphenyl)-1 -(3,4-methylenedioxypheny l)-5-(prop-1 -y loxy)indane-2carboxylate (280 mg, 0.5 mmol) in dry DMF (3 ml) was added to NaH (18 mg,

0.6 mmol) in a small volume of dry DMF. The mixture was stirred at RT for 20 min. then benzyl bromoacetate (137 mg, 0.6 mmol) was added. After stirring for 1.5 h the product was partitioned between 3M aqueous HCI and ethyl acetate. The organic layer was washed with water then brine, dried (MgSO4 anhyd.) filtered and evaporated to give an oil. The product was purified by column chromatography to provide the title compound (240 mg, 66%) as a colorless oil.

c) 22-DimcthylprQpanoyl9\ymgthyI (lRS.2SR,3RS)-3-(2carboxymethoxy-4-methoxyphenyl)-1 -(3.4-methylenedioxvphenyn-5-(prop-1 ylQxy)indanc-2-garboxylic add·

2,2-DimethylpropanoyloxymethyI (1 RS,2SR,3RS)-3-(2carbobenzyloxymethoxy-4-methoxyphenyl)-1 -(3,4-methylenedioxyphenyl)-515 (prop-l-yloxy)indane-2-carboxylate (240 mg, 0.3 mmol) was dissolved in a 2:1 mixture of ethyl acetate and ethanol (3 ml) and then 50 mg of 10%Pd/C was added. The mixture was stirred at room temperature under an H2 atmosphere for 3 h. The catalyst was then filtered, the solvent concentrated in vacuo and the resultant oil purified by flash column chromatography. The title compound was obtained (180 mg, 86%) as a colorless oil.

MS (exact mass) (M+Na)⁺·: 647.2335 (sodium salt) (D = -2.3. mDa for C33H38O1 jNa) mp 190-195°C (dec, sodium salt)

EXAMPLE 5 (lS.2R.3S)-3-r2-rCarbo-(lRS)-l-(2-methoxv-2-methvlpropionvloxv)eth-lylQxymethoxyl-4-methoxyphenyl-l-(3.4-methlenedioxyphenyl)-5-prop-lyloxv^,)indane-2-carboxylic acid sodium salt

a) Allyl (IS. 2R. 3S)-3-(4-methoxy-2-methoxymethoxyphenvn-1-(3.4mcthytengdi9xyphgnyl)-g-(prop-1 -ytoxy)indapg-2-cartoxytotg.

(IS, 2R, 3S)-3-(4-methoxy-2-methoxymethoxyphenyl)-l-(3,4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylic acid (4.8 g, 9.5 mmol) was dissolved in dry acetonitrile (30 ml) and DBU (1.7 ml, 11.4 mmol) was added followed by allyl bromide (3.4 g, 28. mmol). After stirring for 0.5 h. the product was partitioned between 3M aqueous HCI and ethyl acetate. The _organic layer was washed with water and brine, then dried (MgSO4 anhyd.) c

Oi

Cm

O>

-27filtered and evaporated to give an oil. The product was purified by column chromatography to provide the title compound as a pale yellow oil (5.7 g, quantitative).

b) Allyl (IS. 2R. 3S)-3-(2-hydroxy-4-methoxyphenyl)-1-(3.4methylenedioxyphenyB-5-(prop-l-yloxy)indane-2-carboxylate.

Allyl (IS, 2R, 3S)-3-(4-methoxy-2-methoxymethoxyphenyl)-l(3,4-methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-2-carboxylate (1.7 g,

3.11 mmol) was dissolved in allyl alcohol (20 ml) and then 15 drops of cone.

HCI was added. The resulting solution was stirred at 65°C for 2 h. After removing the solvent the residue was partitioned between water and ethyl acetate. The organic layers were washed with water, 5% aqueous NaHCO3 and brine; then dried (MgSO4 anhyd.), filtered and evaporated to give an oil. The product was purified by column chromatography to provide the title compound as a pale yellow oil (1.26 g, 81 %).

c) Allyl (IS. 2R. 3S)-3-(2-Carbo-l-rm-butoxymethoxy-4methoxyphenyl)-l-(3.4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2carboxvlate.

Allyl (IS, 2R, 3S)-3-(2-Hydroxy-4-methoxyphenyl)-1-(3,4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylate (1.0 g, 2 mmol) in dry DMF (4 ml) was added to NaH (57 mg, 2.4 mmol) in a small volume of dry DMF. The mixture was stirred at RT for 20 min., then rm-butyl bromoacetate (974 mg, 5 mmol) was added. After stirring for 0.5 h., the ζ 25 product was partitioned between 3M aqueous HCI and ethyl acetate. The organic layer was washed with water, brine and dried (MgSO4 anhyd.), filtered and evaporated to give an oil. The product was purified by column chromatography to provide the title compound (1.1 g, 93%) as a pale yellow oil.

d) Allyl (IS, 2R. 3S)-3-(2-carboxvmethoxv-4-methoxyphenyl)-l-f3.4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxvlate.

Allyl (IS, 2R, 3S)-3-(2-carbo-tert-butoxymethoxy-4methoxyphenyl)-1 -(3,4-methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-235 carboxylate (765 mg, 1.24 mmol) was dissolved in TFA (5 ml) containing a few drops of anisole. The reaction mixture was stirred at RT for 20 min. The solvent was eliminated, the residue was diluted with ethyl acetate, washed with

-28AP.00573 water, brine and dried (MgSO4 anhyd.), filtered and evaporated. The product was purified by column chromatography to provide the title compound (575 mg, 83%) as a colorless oil.

e) Allyl (IS. 2R. SSl-S-rZ-rCarbo-flRSll-tt-methoxv-Zmcthylpropionyloxyleth-1 -yloxymethoxvl-4-methoxyphenyI-1 -(3.4methlenedioxvphenvl)-5-prop-l-vloxv)indane-2-carboxylic acid sodium salt

Allyl (IS, 2R, 3S)-3-(2-carboxymethoxy-4-methoxyphenyl)-l(3,4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylate (287 mg,

0.5 mmol) was dissolved in DMF (5 ml) and CS2CO3 (333 mg, 1 mmol) added followed by (1S>1-bromoethyl 2-methoxy-2-methylpropionate (225 mg, 1 mmol). The reaction mixture was stirred at RT overnight, then partitioned

C between water and ethyl acetate, washed with dil. HCI and brine, dried (MgSO4 ( anhyd.), filtered and evaporated to give an oil. The product was purified by column chromatography to provide the title compound (260 mg, 74 %) as a colorless oil.

f) (IS. 2R. 3S)-3-r2-iCarbo-flRS)-l-(2-methoxy-2methylpropionyloxykth-l-yloxymethoxyl-4-methoxyphenyll-1 -(3.420 methvlenedioxvphenvl)-5-prop-l-vloxv)indane-2-carboxvlic acid sodium salt

Allyl (1 S,2R,3S)-3-[2-Carbo-(l RS)-1 -(2-methoxy-2methylpropionyloxyeth-1 -yloxymethoxy]-4-methoxyphenyl]-1 -(3,4methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-2-carboxylate (260 mg,

0.37 mmol) was dissolved in CH2CI2 (2 ml) and tetrakis(triphenylphosphine)palladium(0) (36 mg, 0.037 mmol) added followed by tri-n-butyltin hydride (0.11 ml, 0.4 mol). The reaction mixture was stirred at RT for 3 h then quenched with 3N HCI and stirred for 20 min. The organic layer was diluted with ethyl acetate washed with water then brine, dried (MgSO4 anhyd.), filtered and evaporated to give an oil. The product was purified by column chromatography to provide the title compound (210 mg, 85 %) as a colorless oil.

MS (exact mass) (M+Na)⁺·: 687.2415 (sodium salt) (D = +0.3. mPa for C35H4QOj2Na)

AP/P/ y 4 / 0 0 6 3 6

By the methods given above, the following compounds were made.

(IRS. 2SR, 3RS)-3-i2-i(3-Carboxypyridin-2-yl)oxyl-4-methoxyphenyl1-1-(3.4methylenedioxvphenvl)-5-(prop-1 -yloxy)indane-2-carboxvlic acid.

m.p. 152-155°C (1RS.2SR. 3RS)-3-r2-rCarbo(N.N-diethylcarbamoynmethoxylmethoxy-4methoxyphenyIl-l-(3.4-methylenedioxyphenyl)-5-(prop-l-yIoxy)indane-2carboxvlic acid.

m.p. 181-182°C;

Trans. Trans-1,3-Bis(4-methoxyphenyl)indane-2-carboxamide.

C m.p. 223-225°C;

( (1 RS.2SR.3SR)-3-i4-Methoxy-2-i2-(methylphosphinyl) eth-1 -yllphenvll-1 -(3.4methylenedioxyphenvl)-5-(prop-l-yloxy )indane-2-carboxylic acid disodium salt;

(exact mass) M⁺+Na: 619.1462 (Δ = -1.2 mDa for C3()H3iOgPNa3)

EXAMPLES .fc2Q

Indan-5-yl (1 RS.2SR.3RS)-3-(2-carboxymethoxy-4-methoxyphenyD-1-(3.4methylenedioxyphenyl)-5-(prop-1-yloxy )indane-2-carboxvlate sodium, salt,

m.p. 181-183°Cdec.

3. 5-Dimethoxyphenvl (lRS.2SR.3RS)-3-(2-carboxymethoxv-425 . methoxyphenyl)-l-(3.4-methylenedioxyphenyl)-5-(prop-l-yloxy )indane-2r carboxylate sodium salt ' m.p. 185-189°C dec.

(IRS)-1- (2-Methoxy-2-methylpropionyloxy)eth-l-yl (lRS.2SR.3RS)-3-(230 carboxymethoxy-4-methoxyphenyl)- l-(3.4-methylenedioxyphenyI)-5-(prop-1 yloxy)indane-2-carboxvlate sodium salt

m.p. 178-181°Cdec.

- 30AP.0 0 5 7 3 oo

N.N-Dimethylcarbamoylmethyl (lRS.2SR.3RS)-3-(2-carboxymethoxy-4methoxyphenyl)-l-(3.4-methylenedioxyphenvl)-5-(prop-l-yloxy^>>indane-2carboxylate sodium salt

m.p. I70-174°C dec.

Ethoxycarboxymethyl (1 RS.2SR.3RS)-3-(2-carboxymethoxy-4methoxyphcnyl)-1 -(3.4-methylenedioxvphenyn-5-(prop-1 -yloxy)indane-2caiteaylatc sodium salt

MS (exact mass) (M+Na)⁺·: 645.1959 (sodium salt) (D =-1.1 mDa for C33H340i₂Na)

Benzoyloxymethyl (!RS.2SR.3RS)-3-(2-carboxymethoxy-4-methoxyphenyl)-l(3.4-methylcncdioxyphenyl)-5-(prop- l-yloxy)indane-2-carboxylate sodium salt C MS m/e : 672(M+NH4)+

Cyclohexyloxycarboxymethyl (lRS.2SR.3RS)-3-(2-carboxymethoxv-4methoxyphenyl)-l-(3.4-methylenedioxyphenyl)-5-iprop-l-yloxy)indane-2eartoxylatc sodium salt

MS (exact-mass) (M+Na)⁺·: 699.2453 (sodium salt) (D = -3.5. mDa for C37H4QOj₂Na)

Ethyl f lRS.2SR,3RS)-3-(2-carboxymethoxy-4-methoxyphenyn-1 -(3.4methvlenedioxvphenvl)-5-(prop-l-vloxv)indane-2-carboxylate sodium salt <=.

MS (exact mass) M⁺·: 548.2040 (free acid) ® (D =+0.6. mDa for ¢311^()9) (lS.2R.3S)-3-r2-rCarbo-f2'.6^,-dimethylphenoxy)methoxyl-4-methoxvphenyll-l(3.4-methvlenedioxvphenvl)-5-(prop-1 -yloxy)indane-2-carboxylic acid sodium salt

MS (exact mass) (M+Na)⁺·: 647.2264 (sodium salt)

D = -0.7. mDa for (^F^C^Na) flS.2R.3S)-3-f2-(Carbocyclopentyloxymethoxy)-4-methoxyphenyn-l-(3.4methvlenedioxyphenyl)-5-(prop-l-yloxylindane-2-carboxylic acid sodium salt

MS (exact mass) (M+Na)⁺·: 611.2282 (sodium salt) (D = -2.5. mDa for ^341135()9^)

-31 (lS.2R.3S)-3-12-fCarbo(indan-5-yloxy)methoxy]-4-methoxyphenylI-l-(3.4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylic acid sodium salt

MS (exact mass) (M+Na)⁺· : 659.2281 (sodium salt) (D = -2.4. mDa for C3gH3bO9Na) (lS.2R.3S)-3-r2-Carbo(eth-l-yloxy)methoxyl-4-methoxyphenyll-l-(3.4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylic acid sodium salt

MS (exact mass) (M+2Na-H)⁺· : 593.1769 (sodium salt) (D =-0.6 mDa for C3iH3iO9Na2) (lRS.2SR.3RS)-3-(2-Carboethoxymeihoxv-4-methQXYDhenvl)-l-(3.4methylenedioxyphenyl)-5-(prop-l -yloxy)indane-2-carboxylic acid

m.p. 148-149°C.

(IRS. 2SR, 3RS)-2-TrifluoromethvIsulfonamidomethvl-3-(2-carboxvmethoxv4-methoxvphenyl)-l-(3.4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane

m.p. 184-186°C

Indan-5-yl (1 S.2R.3S)-3-(2-carboxymethoxy-4-methoxyphenyl)-1 -(3.420 methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-2-carboxylate sodium salt

CyclopentylflS.2R.3S)-3-(2-carboxvmethoxY-4-methoxyphenvI)-l-(3.4methylenedioxvphenyl)-5-(prop-l -vloxy)indane-2-carboxvlate sodium salt. c?

C

Ethoxycarboxymethvl (lS.2R.3S)-3-(2-carboxymethoxy-4-methoxyphenyl)-l(3.4-methylenedioxvphenyl)-5-(prop-1 -yloxv)indane-2-carboxylate sodium salt, (IRS)-1 -(1 -Methylethoxycarboxvleth-1 -yl (1 RS.2SR.3RS)-3-(2carboxymethoxv-4-methoxyphenyl)-l-(3.4-methylenedioxyphenvl)-5-(prop-l30 yloxv)indane-2-carboxvlate sodium salt. m.p. 151-155°C.

Ethyl (lS.2R.3S)-3-(2-carboxymethoxv-4-methoxvphenvl)-l-(14methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylate sodium salt

- 32AP . Ο Ο 5 7 3 flS.2R.3S)-3-r2-Carbomethoxvmethoxy-4-methoxvphenvll-1-(3.4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylic acid sodium salt nS.2R.3S)-3-r2-rCarbobenzovloxymethoxvmethoxyl-4-methoxyphenvn-l5 (3.4-methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-2-carboxvlic acid sodium salt (lS.2R.3S)-3-f2-(Carboethoxvcarboxyloxvmethoxymethoxy)-4mcttoxyphcnyll· 1 -(3.4-mctfiylcngdiQxyphcnyl)-5-(prop,d-ylgxy)indang-210 carboxylic acid sodium salt (lS.2R.3S)-3-r2-(Carboacetoxymethoxymethoxy)-4-methoxyphenyn-l-(3.4methylenedioxvphenvl)-5-(prop-l-vloxv~)indane-2-carboxvlic acid sodium salt (lS^R.3S)-3-r2-iCarbophthalidylmethoxy)-4-methoxyphenyn-l-(3.4methylenedioxyphenyl)-5-(prop-l-yloxy)indane-2-carboxylic acid sodium salt

QS.2R3S)-3J2-Carfao-(2-methoxv-2-methylpropionvIoxymethoxymethoxyl-4methoxyphenyIl-l-(3.4-methylenedioxyphenyl)-5-(prop-l-yloxy)indane-220 carboxylic acid sodium salt

EXAMPLE 31 (1 S2R3S)-3-f2-CarbQ-('2.2-dimethvlpropanovloxvmethoxymethoxvl-425 methoxyphenyl1-l-(3.4-methylenedioxyphenyP-5-(prop-l-yloxy)indane-2carbo&ylic acid sodium salt

To a solution of (lS,2R,3S)-3-[2-Carboxymethoxy-4-methoxyphenyl]-l-(3,4methylenedioxyphenyl)-5-(prop-1 -yloxy)indane-2-carboxylic acid monopotassium salt (170 mg, 0.3 mmol) (obtained by treatment of the corresponding diacid (156 mg, 0.3 mmol) with 1 equiv. of KHCO3 (30 mg, 0.3 mmol)) in DMF (4 mL) pivaloyloxymethyl iodide (74 mg, 0.3 mmol) was added. The reaction mixture was stirred at RT for 0.5 h and more pivaloyloxymethyl iodide was then added (20 mg. 0.08 mmol) and then stirred for an additional 0.5 h. The reaction mixture was partitioned between dilute aqueous HCI and ethyl acetate. The ethyl acetate extract was washed with water and brine and dried (MgSO4 anhydrous). The solvent was removed in vacuo and the residue was purified by flash column chromatography

-t

c.0 cr.

Cr o>

-3310 c

r (silica gel, Ethyl Acetate/hexane/HOAc 30/65/5) to obtain the desired compound as a white foam (140 mg, 73 %) as the free acid which was converted to its sodium salt. m.p. 128 - 133°C.

MS (exact mass) M⁺+Na : 679.2116 (sodium salt) (D = +1.2 mDa for C35H370jiNa2)

EXAMPLE 32

Formulations for pharmaceutical use incorporating compounds of the present invention can be prepared in various forms and with numerous excipients. Examples of such formulations are given below.

Inhalant Formulation

A compound of Formula I, (1 mg to 100 mg) is aerosolized from a metered dose inhaler to deliver the desired amount of drug per use.

Tablgts/Ingredisnts Esr Table!

c ²⁵ (

Active ingredient	40 mg
(Cpd of Form. I)
Com Starch	20 mg
Alginic acid	20 mg
Sodium Alginate	20 mg
Mg stearate	13 mg
	2.3 mg

Procedure for tablets;

Step 1 Blend ingredients No. 1, No. 2, No. 3 and No. 4 in a suitable mixer/blender.

-34^

AP.005 7 J

Step 2 Add sufficient water portion-wise to the blend from Step 1 with careful mixing after each addition. Such additions of water and mixing until the mass is of a consistency to permit its conversion to wet granules.

Step 3 The wet mass is converted to granules by passing it through an oscillating granulator using a No. 8 mesh (2.38 mm) screen.

Step 4 The wet granules are then dried in an oven at 10 140°F (60°C) until dry.

Step 5 The dry granules are lubricated with ingredient No. 5.

Step 6 The lubricated granules are compressed on a suitable tablet press.

Parenteral Formulation

A pharmaceutical composition for parenteral administration is prepared by dissolving an appropriate amount of a compound of formula I in polyethylene glycol with heating. This solution is then diluted with water for injections Ph Eur. (to 100 ml). The solution is then steriled by filtration through a

0.22 micron membrane filter and sealed in sterile containers.

Claims (9)

1. A compound of Formula (I):

(I) wherein:

Rl is -X(CH₂)_nAr or -X(CH₂)_nRg or

AP/P/ 9 A / 0 0 6 3 6

R₂ is hydrogen, Ar, C^alkyl or (c);

Pi is -X(CH₂)_nRg;

P₂ is -X(CH₂)_nRg, or -X-R9-Y;

R3 and R5 are independently hydrogen, R11, OH,

Ci.galkoxy, S(O)_qRi μ N(Rg)₂, Br, F, I, Cl, CF3, NHCORg, Ri iCO₂R7,

-X-R9-Y, or -X(CH₂)_nRg wherein each methylene group within

-X(CH₂)_nRg may be unsubstituted or substituted by one or two -(CH₂)_nAr groups;

R4 is hydrogen, Rn, OH, Ci^alkoxy, S(O)_qRn,N(R6)₂, -X(Rn),

Br, F, I, Cl or NHCORg wherein the Ci^alkoxy may be unsubstituted or substituted by OH, methoxy or halogen;

R5 is independently hydrogen or C^alkyl;

R7 is independently hydrogen, Ci_ioalkyl, C₂_ioalkenyl or C₂.g alkynyl, all of which may be unsubstituted or substituted by one or more OH,_

- 36 - ...AP . Ο Ο 5 7 3

N(Rg)2’ CO2R12. halogen or XC galkyl; or R7 is (CH2)_nAr;

Rg is hydrogen, Ri j, CO2R7, CC>2C(Ri i)2O(CO)XR7, PO3(R7)2,

SO2NR7R11, CONR7SO2R11, SO₃R₇, SO2R7, P(O)(OR₇)R₇, CN, C(O)N(R₆)₂,

-CO2(CH2)_mC(O)N(R6)2, C(Ri 1^^7)2, tetrazole or ORg;

5 R9 is (CH₂)_n> divalent C^qQalkyl, divalent C2-ioalkenyl ^or phenyl, all of which may be unsubstituted or substituted by one or more OH, N(Rg)2, COOH, halogen, or may be )C=O or XCj^alkyl;

RlO is R3 or R4;

Rll is hydrogen, Ar, C^galkyl, C₂-8alkenyi, C₂-galkynyl, all of

10 which may be unsubstituted or substituted by one or more OH. CH₂OH, N(Rg)2 or halogen;

Rl2 is hydrogen, Ci.galkyl, C2-galkenyl or C2-7alkynyl;

X is (CH2)_n, O, NRg or S(O)_q;

Y is CH3 or X(CH₂)_nAr;

r z

naphthyl, indolyl, pyridyl, thienyl, oxazolidinyl, oxazolyl, thiazolyl, 20 isothiazolyl, pyrazolyl, triazolyl, tetrazolyl, imidazolyl, imidazolidinyl, thiazolidinyl, isoxazolyl, oxadiazolyl, thiadiazolyl, morpholinyl, piperidinyl, piperazinyl, pyrrolyl, or pyrimidyl; all of which may be unsubstituted or substituted by one or more R3 or R4 groups;

A is C=O, or [C(Rg)2]_m;

25 B is -CH2- or -O-;

Zj and Z2 are independently hydrogen, Cj. galkyl, C2-galkenyl, C2-8alkynyl, OH, Cvgalkoxy, S(O)qCi.galkyl, N(Rg)2, Br, F, I, Cl, NHCORg, X-R9-Y,

-X(CH2)_nR8’ phenyl, benzyl or C3_gcycloalkyl wherein the Cj.galkyl,

30 C2-8alkenyl or C2-galkynyl may be optionally substituted by COOH, OH, c

c cr

O*·

σ.

H5(X)42-4rr

CO(CH2)_nCH3, CO(CH2)_nCH2N(R6)2, or halogen; or Zj and Z2 together may be -O-A-O- on contiguous carbons;

Z3 is Zj or -X-R9-Y; q is zero, one or two; n is an integer from 0 to six;

m is 1,2 or 3;and the dotted line indicates the optional presence of a double bond; or a pharmaceutically acceptable salt thereof; provided that

R2 is not hydrogen when X is S(O)q;

when the optional double bond is present there is only one Rjq and there is no Pj and P2 is not NRgRgY;

when the optional double bond is present in Formula (I) and X-R2 is attached to the double bond, X is not NRg;

when the optional double bond is present and Rj is attached directly to the double bond, Ri is not NR^AR;

when R3, R5, Zj, Z2, or Z3 is X(CH2)_nRg and n is not 0, X is oxygen or NR$ when Rg is ORg or CO2H;

when Rg is CO2C(Ri j)2O(CO)XR7_> X is not S(O)q;

• the compound of Formula I is not (IRS)- l,3-diphenylindene-2carboxylic acid; (cis,cis)-(lRS,3SR)-l,3-diphenylindane-2-carboxylic acid; (lRS)-3-[3-Methyl-l-phenyl-(lH)-ind-2-en-!-yl] propionic acid; or (lRS)-2[l,3-diphenyl-(lH)-ind-2-en-2-yl]ethanoic acid; 1,3diphenyl-l-ethoxyindene-2-carboxylic acid; 1,2,3-triphenylindene; 1,3 diphenylindene; l-(2,3-dimethyl-2-buten-yl)-l,3-diphenylindene; 1,3diphenyl-2-methylindene; l,3-diphenyl-2-methylindane; 1,3diphenylindane; 5,6-dimethoxy-l,3-dimethoxyindene; l,3-bis(4,5dimethoxy-2-hydroxyphenyl)-5,6-dimethoxyindane; 1,3-bis(3,4dimethoxyphenyl)-5,6-dimethoxyindane; 1,3-diphenyl-2methoxyidene, l,3-diphenyl-2-ethoxyindene, or 5-fluoro-2-methylindene-3-acetic acid;

and further provided that compounds :

Methyl-1,3-diphenylindene-2-carboxylate;

1,3-diphenyl-2-(4-methoxyphenyl)-H-indene; and compounds wherein

Rl is -X(CH₂)_nAr or -X(CH₂)_nR8 or tt

O'.

OM

Ch

-38AP . Ο Ο 5 7 3

R2 is hydrogen, Ar or (c);

Pl is -X(CH₂)_nR8;

P₂ is -X(CH₂)_nR8> ^or -XR9Y;

R3 and R5 are independently hydrogen, Rj p OH, Ci_galkoxy,

5 S(O)qRn, N(Rg)2, Br, F, I, Cl, CF3, NHCORg, -XR9-Y or -X(CH2)_nR8 wherein the methylene groups of

-X(CH2)_nR8 ^may be unsubstituted or substituted by one or more -(CH2)_nAr groups;

R4 is hydrogen, Rj j, OH, Cj^alkoxy, S(O)qRn, N(Rg>2, -X(Rn),

10 Br, F, I, Cl or NHCORg wherein the

C 1.5alkoxy may be unsubstituted or substituted by OH, methoxy or halogen;

R6 is independently hydrogen or Cj^alkyl;

R7 is independently hydrogen, Cj.galkyl or (CH2)_nAr,

Rg is hydrogen, Rj j, CO2H, PO3H2, P(O)(OH)R7 or tetrazole;

15 R9 is C1. lOalkyl· 10^a^^ceny^^or phenyl all of which may be unsubstituted or substituted by one or more OH, N(Rg)2, COOH, halogen or XCp 5alkyl;

RlO is R3 or R4;

Rj j is Cμgalkyl, C2-8alkenyl, C2-8alkynyl all of which may be

20 unsubstituted or substituted by one or more OH, CH2OH, N(Rg)2 or halogen;

X is (CH2)_n, O, NRg or S(O)_q;

Y is CH3 or -CH₂X(CH2)_nAr,

Ar is:

/R₃ '/□ *

• 1 <0

Ti (a)

Si naphthyl, indolyl, pyridyl or thienyl, oxazolidinyl, oxazolyl, thiazolyl, isothiazolyl, pyrazolyl, triazolyl, tetrazolyl, imidazolyl, imidazolidinyl, thiazolidinyl, isoxazolyl, oxadiazolyl, thiadiazolyl, morpholinyl, piperidinyl, piperazinyl, pyrrolyl,

-39or pyrimidyl; all of which may be unsubstituted or substituted by one or more R3 or R4 groups;

A is C=O, or [C(R6)2l_m;

B is -CH2- or -O-;

5 Zi and Z2 are independently hydrogen,

Ci.galkyl, C2-galkenyl, C2.galkynyl, OH, Ci^alkoxy, S(O)qCj.galkyl, N(Rg>2, Br, F, I, Cl, NHCOR₆,

-X(CH2)_nRg, phenyl, benzyl or C3.gcycloalkyl wherein the Ci.galkyl, C2-galkenyl or C2-galkynyl may be optionally substituted by COOH, OH, COiCH2)_nCH3,

10 CO(CH2)_nCH2N(R6)2>^or halogen; or Zi and Z2 together may be -Ο-A-O- on contiguous carbons;

Z3 isZi or XR9Y; q is zero, one or two; n is an integer from 0 to six;

15 mis 1,2or 3; are excluded.

2. A compound of Claim 1 wherein Ri is X(CH2)_nAr, dihydrobenzofuranyl, benzodioxanyl, cyclohexyl, or Ci^alkyl; R2 is a moiety of formula (a) or (b), Ci_4alkyl, indolyl or hydrogen; R3 and R5 are independendy

20 hydrogen, OH, C1.5alkoxy, halogen, -OC1 ^alkyl phenyl, R11CO2R7, C1 ^alkyl,

N(Rg>2, NH(C0)CH3, -X(CH2)_nRg. -X-R9-Y, pyridyl, phenyl or S(0)qCi_5alkyl; R4 is hydrogen, OH, Ci^alkoxy, halogen, Ci^alkyl, N(Rg)2, NH(CO)CH3 or S(O)qCi_5alkyl; Ζμ Z2 and Z3 are independently X-Rq-Y, benzyl, hydrogen, OH, Ci_5alkoxy, -N(Rg)2, S(O)qCi_galkyl, NHCORg, X(CH2)_nR8 or halogen, or Zi

25 and Z2 together may be -0-A-0 on contiguous carbons; Pi and P2 are independently hydrogen, CO2H or tetrazole; Ar is a moiety of formula (a), or (b), phenyl, or pyridyl and X is (CH2)_n or oxygen.

3. A compound of Claim 2 wherein R3 is hydrogen, -X(CH2)_nR8 or

30 Ri 1CO2R7; R

4 and R5 are independently hydrogen, OH, Cj^alkoxy, SCi^alkyl, substituted phenyl, F, Br, C^alkyl or NH2; Zj and Z3 are hydrogen and Z2 is hydrogen, OH, Ci^alkoxy, halogen, X(CH2)_nRg, NH2, benzyl or NH(CO)CH3, or Zi and Z2 together may be O-A-O on contiguous carbons.

35 4. A compound of Claim 3 wherein Ri is a moiety of formula (b) and

R2 is a moiety of formula (a) or (b); A is CH2, B is -O-; there is no optional double bond; Ri and XR2 are trans to Ρμ Z2 is OH, Ci^alkoxy, -OCH2CHCH3 or

J

R u

-t c

c

C

-40AP.00573 hydrogen, Zj is hydrogen; R3 is hydrogen, XAr, X(CH2)qCO2H, X(CH2)qCONR7SO2Rn, orCH=CHCO2H, R4 is hydrogen, substituted phenyl, or Cj^alkoxy; and R5, Rjq and P2 are hydrogen.

5 5. A compound selected from the group consisting of:

(+)(1RS, 2SR, 3SR)-3-(2-Carboxymethoxy-4-methoxyphenyl)-l-(3,4methylenedioxyphenyl)-5-(prop’l-yloxyy)indane-2-carboxyIic acid; and

10 (+)(1RS, 2RS, 3SR)-3i2-(2-Hydroxyeth-l -yloxy)-4-methoxyphenyl]-l-(3,4methylenedioxyphenyI)-5-(prop- l-yloxy)indane-2-carboxylic acid;

(IRS, 2RS, 3SR)-3-[2-[(4-Carboxypyridin-3yl)oxy]'-4-methoxyphenyI]-l(3,4>methylenedioxyphenyl)-5-(prop-l *yloxy)indane-2-carboxylic acid disodium.

6. A pharmaceutical composition comprising a compound of Claim 1 or Claim 5 and a pharmaceutically acceptable carrier.

7. A method of antagonizing endothelin receptors which comprises 20 administering to a subject in need thereof, an effective amount to antagonize endothelin receptors of a compound of Claim 1 or Claim 5.

8. A method of treating hypertension, renal failure or cerebrovascular disease which comprises administering to a subject in need thereof, an effective

25 amount of a compound of Claim 1 or Claim 5.

AP/P/ 94/0063«

9. A process for the preparation of a compound of formula (I) of Claim 1 or Claim 5 a pharmaceutically acceptable salt thereof, which process comprises reacting a compound of formula (Π)

-41 wherein Ζμ Z₂, Z3 and R} are as described in claim 1 or a group convertable thereto, and X is alkyl, with an organoraagnesium compound of formula (ΙΠ)

R2-(CH₂)_n-MgBr (IH) wherein R₂ is as described in claim 1 or a group convenable thereto, in a suitable solvent to provide a compound of formula (IV) which is reduced and thereafter, when desired or necessary undergoes,

a) insertion of R}q (when other than hydrogen) through conjugate additon;

and/or

15 b) alkylation or acylation to give compounds wherein Pi and P₂ are other than CO₂H; and/or

c) conversion R j, R₂, Ζμ Z₂ and Z3_; to afford a compound of formula (I).