AP492A - 1,5 Benzodiazepine derivatives. - Google Patents

Abstract

Compounds of formula

Description

1,5-Benzodiazepine Derivatives 5

This invention relates to novel 1,5-benzodiazepine derivatives, to processes for their preparation, to pharmaceutical compositions containing them and to their use in medicine.

Cholecystokinins (CCK) and gastrin are structurally related peptides which exist in gastrointestinal tissue and in the central nervous system. Cholecystokinins include CCK-33, a neuropeptide of thirty-three amino acids in its originally isolated form, its carboxy terminal octapeptide sulphate, CCK-8 (also a naturally-occurring neuropeptide), and 39- and 12-amino acid forms. Gastrin occurs in 34-, 17- and 14- amino acid forms, with the minimum active sequence being the C-terminal tetrapeptide, Trp-Met-Asp-Phe-NH₂(CCK-4), which is the common structual element shared by both CCK and gastrin.

CCK and gastrin are gastrointestinal hormones and neurotransmitters in the neural and peripheral systems and perform their respective biological roles by binding to particular receptors located at various sites throughout the body.

There are at least two subtypes of cholecystokinin receptors termed CCK-A and CCK-B and both are found in the periphery and in the central nervous system.

CCK and gastrin receptor antagonists have been disclosed for preventing and treating CCK-related and/or gastrin related disorders of the gastrointestinal and central nervous systems of animals, and more particularly humans.

US Patent No. 4,988,692 describes a group of 3-acylamino 1-alkyl-5-phenyl 1,530 benzodiazepine derivatives as cholecystokinin antagonists. Further the specification teaches that the compounds have a significantly greater affinity for the CCK-A receptor over the CCK-B receptor.

We have now found a novel group of 3-substituted 1,5-benzodiazepine

AF/P/ 9 4 / 0 0 6 5 7

1160CH

AP. 0 0 4 9 2 compounds which are potent and specific antagonists of gastrin and/or CCK and in particular antagonists of gastrin and /or CCK at the CCK-B receptor which exhibit a particularly advantageous profile of activity.

Thus, the invention provides compounds of general formula (I)

NHCOXR (D wherein

R¹ represents a phenyl, C3.7cycloalkyl, C7.1 -j bridgedcycloalkyl or C-i^alkyl group which alkyl group may be substituted by a hydroxy, phenyl, C-j. galkoxycarbonyl, C3_7cycloalkyl, or C7.-J 1 bridgedcycloalkyl group;

R² represents a phenyl group optionally substituted by 1 or 2 substituents selected from, halogen, CMalkyl, CMalkylthio, cyano, nitro, trifluoromethyl, trifluoromethoxy, (CH2)nR⁴ or O(CH2)pR⁴ wherein R⁴ represents hydroxy, C-). ₄alkoxy, CO₂R⁵ or NR⁶R⁷; n is zero or 1; p is an integer from 1 to 4;

R³ represents the group AlkNR⁸R⁹;

R⁵ represents hydrogen or C_Malkyl;

R⁶ represents hydrogen or C_Malkyl;

R⁷ represents hydrogen, C^alkyl, acyl, or C^alkyl substituted by one or more hydroxy, carboxyl and/or amino groups or R⁶ and R⁷ together with the nitrogen atom to which they are attached form a 5-7 saturated heterocyclic ring which contain an additional heteroatom selected from oxygen, sulphur or nitrogen and/or may be substituted by 1 or 2 C_Malkyl or hydroxy groups.

R⁸ and R⁹ independently represent hydrogen, C^alkyl or C^alkyl substituted by one or more hydroxy, carboxyl and/or amino groups or R⁸ and R⁹ together with the nitrogen atom to which they are attached represent a 5-7 saturated heterocyclic ring which may contain an additional heteroatom selected from oxygen, sulphur or nitrogen and/or may be substituted by 1 or 2 Chalky I or hydroxy groups; Aik represents a straight or branched C₂^alkylene chain optionally substituted by an hydroxyl group;

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1160CH

R¹⁰ represents hydrogen or a halogen atom; m is zero, 1 or 2;

X is oxygen or NH; and pharmaceutically acceptable salts and or metabolically labile esters thereof.

It will be appreciated that compounds of formula (I) possess at least one asymmetric carbon atom (namely the carbon atom occupying the 3-position of the diazepine ring) and the compounds of the invention thus include all stereoisomers and mixtures thereof including the racemates.

In the compounds of formula (I) 'alkyl' when used as a substituent or part of a substituent group means that the group may be straight or branched. Thus, Ci_e alkyl includes methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl or tertbutyl, n-pentyl, isopentyl, neopentyl, n-hexyl, isohexyl, 1,3-dimethylbutyl, 3,3dimethylbutyl, 2,3-dimethylbutyl.

For the group R¹ the term C3_7cycloalkyl as a group or part of a group refers to a monocyclic alkyl group such as cyclopropyl, cylobutyl, cyclopentyl, cyclohexyl or cycloheptyl. The term C7.11 bridged cycloalkyl refers to groups such adamantyl, norbomanyl or norbornenyl.

For the groups R⁵· R⁶· R⁷ R⁸ and R⁹ the term Cj^alkyl includes 3-4- cycloalkyl (eg. cyclopropyl or cyclobutyl) as well as straight or branched chain alkyl groups as defined above.

Halogen in the definition of compounds of formula (I) may represent a fluoro, chloro, bromo or iodo substituent.

When R? is a phenyl group substituted by a single substituent this may be in the ortho, or more preferably the meta or para position.

When the group R⁶ and R⁷ together with the nitrogen atom represent a saturated heterocylic group examples of suitable groups include morpholino,

2,6-dimethylmorpholino, thiomorpholino, piperidino, 4,4-dimethylpiperidino and pyrrolidino.

AP/P/ 94/00657

1160CH

AP.00492

When R⁶ and R⁷ are both alkyl they are conveniently the same e g methyl.

When R⁷ represents acyl this may be for example C^alkanoyl e g. formyl or acetyl.

When Aik represents a straight or branched C₂^alkylene chain examples of such groups include ethylene, 2-methylethylene, propylene, butylene, pentamethylene or hexamethylene.

When Aik represents a C₂^alkylene chain substituted by a hydroxyl group examples of such chains include ethylene or propylene optionally substituted by hydroxymethyl, e g. 2 hydroxymethyl-ethylene

When R⁸ and R⁹ together with the nitrogen atom to which they are attached represent an heterocylic group examples of suitable groups include morpholino,

2,6-dimethylmorpholino, hexamethyleneimino, piperidino, pyrrolidino, piperazino or N-methylpiperazino.

When R⁸ and R⁹ independently represent Chalky I examples of such groups include methyl or ethyl

AP/P/ 94/00657

When R1O is halogen this is preferably chlorine or fluorine.

When m is 1 or 2 the halogen atom(s) e g. chlorine or fluorine are preferably in the 7 and/or 8 positions.

When R¹ represents an alkyl group substituted by a hydroxyl group this is preferably a C3^alkyl group substituted by hydroxy. Examples of such groups include 2-hydroxypropyl, 2-hydroxy-3-methylbutyl and 2-hydroxy-3,3dimethylbutyl of which 2-hydroxy-3-methylbutyl, and 2-hydroxy-3,3-dimethylbutyl are particularly preferred.

When R1 represent an alkyl group substituted by a C3_7cycloalkyl group this is preferably a C-|_3alkyl group such as methyl, ethyl or 1-methylethyl, substituted by a C3 7cycloalkyl group such as cyclopentyl, or cyclohexyl.

BAD ORIGINAL ft

11600H

When R¹ is a bridged C7.11 cycloalkyl group this may be for example an adamantyl group such as 1-adamantyl or 2-adamantyl group or a 2-norbornanyl group.

When R¹ is an alkyl group substituted by a bridged C7.1 -j cycloalkyl group this is preferably an ethyl group or more especially a methyl group substituted by a bridged C7.1 j cycloalkyl group. Examples of suitable bridged cycloalkyl groups include adamantyl such as 1 -adamantyl or 2-adamantyl, 2-norbornanyl or 5t 10 norbornenyl. Most preferably R* represents 1-adamantylmethyl.

When R1 is alkyl substituted by phenyl this may be for example benzyl or phenethyl.

When R¹ is alkyl substituted by alkoxycarbonyl this is preferably methyl substituted by alkoxycarbonyl such as methoxycarbonyl or as t-butoxycarbonyl.

Examples of suitable R¹ groups include a phenyl, adamantyl, norbornanyl, phenethyl, C^alkyl e g. n- butyl, 3-methyl butyl, 3,3-dimethyl butyl, 1,320 dimethylbutyl, 2,3- dimethylbutyl, C3.6 hydroxy alkyl e.g. 2-hydroxypropyl, 2hydroxy-3- methylbutyl, 2-hydroxy-3,3-dimethylbutyl, C^alkyl substituted by a bridged C7.1 gcycloalkyl group e g. 2-norbornanylmethyl, 5-norbornenylmethyl, ⁽ 2-adamantylmethyl, 2-adamantylethyl, 2-(1-adamantyl)ethyl, 1adamantylmethyl, alkoxycarbonylalkyl, e.g. methoxycarbonylmethyl or t25 butyoxycarbonyl methyl, cyclohexylmethyl, or 2-cyclopentylethyl.

Conveniently R¹ represents phenyl, cyclohexylmethyl, 3-methylbutyl or 1adamantylmethyl and more particularly 3-methylbutyl or 1-adamantylmethyl.

Examples of suitable R² groups include phenyl or phenyl substituted by one or two groups selected from fluorine, chlorine, bromine, methyl, methoxy, hydroxy,

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1160CH

AP. Ο Ο 4 9 2

Conveniently R² is a phenyl group or a phenyl group substituted in the meta or para position by a single substituent selected from fluorine, chlorine, bromine methyl, methoxy, hydroxy, trifluoromethyl or methylthio.

Preferably R² represents phenyl, 3-methylphenyl, 4-fluorophenyl, 4methoxyphenyl or more especially phenyl.

Conveniently the group X represents NH

Conveniently the group Aik represents ethylene, propylene or 2-hydroxymethylethylene.

Conveniently the group NR®R⁹ represents amino, dimethylamino, diethylamino, morpholino, pyrrolidino, piperidino or hexamethyleneimino.

R¹⁰ conveniently represents fluorine or chlorine or more particularly hydrogen.

Examples of suitable R³ groups include morpholinoethyl, pyrrolidinoethyl, piperidinoethyl, dimethylaminoethyl, diethylaminoethyl, dimethylaminopropyl, amino-propyl, 2-hydroxymethyl-2-aminoethyl or hexamethyleneiminoethyl.

Conveniently R³ represents morpholinoethyl, piperidinoethyl, pyrrolidinoethyl,

Q dimethylaminoethyl, diethylaminoethyl, dimethylamino-propyl or 2hydroxymethyl-2-aminoethyl or hexamethyleneiminoethyl Preferably R³ 25 represents morpholinoethyl.

Examples of suitable compounds of formula (I) are those wherein R^ represents 1-adamantylmethyl and R³ represents morpholinoethyl, X represents NH and, R¹⁰ represents hydrogen

A preferred group of compounds of formula (I) include those wherein R¹ is 1adamantylmethyl, R² is phenyl optionally substituted by halogen e.g. fluorine or bromine, R³ represents, 2-(4-morpholino)ethyl, 2-(1-piperidino)ethyl, 2-(1pyrrolidino)ethyl, 2-(dimethylamino)ethyl, 3-(dimethylamino)propyl, 29 4/ 0 0 6 5 7

hydroxymethyl -2-aminoethyl, 3-aminopropyl, X is NH and R¹⁰ is hydrogen or fluorine and m is 1.

A further preferred group of compounds of formula (I) include those wherein R¹ 5 is 3-methylbutyl, R² is phenyl optionally substituted by methyl, methoxy, chlorine, bromine, fluorine, trifluoromethyl, hydroxy or methoxy, R³ is 2(dimethylamino)ethyl, 2-(diethylamino)ethyl₁2-(1-piperidino)ethyl or 2-(4morpholino)ethyl. X is NH, R¹⁰ is hydrogen or fluorine and m is 1.

Compounds wherein R¹ is cyclohexylmethyl, R² is phenyl or 3-methylphenyl R³ is 2-(diethylamino)ethyl or 2-(4-morpholino)ethyl, X is NH and R¹⁰ is hydrogen represent a further preferred group of compounds of this invention.

Another preferred group of compounds according to the invention include those wherein R¹ is phenyl, R² is phenyl optionally substituted by methyl, fluoro or 315 methylthio, R³ is 2-(1-hexamethylenimino)ethyl, X is NH and R₁₀ is hydrogen.

Preferred compounds of the invention include:

N-[1-(1-Adamantylmethyl)-2,4-dioxo-5-[2-(4-morpholino)ethyl]-2,3,4,520 tetrahydro-1 H-benzodiazepin-3-yl]-N'-phenylurea;

N-[1-(1-Adamantylmethyl)2,4-dioxo-5-[2-(4-morpholino)ethyl]-2,3,4_l5 tetrahydro1 H-benzodiazepin-3-yl]-N^-(4-fluorophenyl) urea.

N-[2,4-Dioxo-1 -(3-methyl-1 -butyl)-5-[2-(4-morpholino)ethyl]-2,3,4,5-tetrahydro1H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

N-[2,4-Dioxo-1 -(3-methyl-1 -butyl )-5-[2-(1 -piperidino)ethyl]-2,3,4,5-tetrahydro1H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

N-{5-(2-(Dimethylamino)ethyl]-2,4-dioxo-1-(3-methyl-1-butyl)-2,3,4,5-tetrahydro1H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

N-[5-{2-(Dimethylamino)ethyl]-2,4-dioxo-1-(3-methyl-1-butyl)-2,3,4,5-tetrahydro35 1 H-1,5-benzodiazepin-3-yl]-N'-(4-methoxyphenyl)urea £5900/V6 /d.'dV

1160CH

AP00492

N-[2,4-Dioxo-1-(3-methyl-1-butyl)-5-[2-(4-morpholino)ethyl]-2,3,4,5-tetrahydro1 H-1,5-benzodiazepin-3-yl]-N'-{4-methoxyphenyl)urea;

N42,4-Dioxo-1-{3-methyl-1-butyl)-5-[2-(4-morpholino)ethyl]-2,3,4,5-tetrahydro1 H-1,5-benzodiazepin-3-yl]-N'-(4-hydroxyphenyl)urea;

N-iS-p^diethylaminoJethylJ^^-dioxo-HS-methyl-l-butyO-J^.SAS-tetrahydro1H-benzodiazepin-3-yl]-N’-phenylurea;

N-[5-[2-diethylamino)ethyl]-2,4-dioxo-1-(3-methyl-1-butyl)-]-2,3,4,5-tetrahydro1 H-benzodiazepin-3-yl]-N’-N’-(4-fluoropheny l)urea;;

N4(1-Adamantylmethyl)-5-[2-(dimethylamino)ethyl]-2,4-dioxo-2,3,4,5-tetrahydro15 1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

N-[1-(1-Adamantyl)methyl-5-[3-(dimethylamino)propyl)-2,4-dioxo-2,3,4,5tetrahydro-1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

N-[1-(1-Adamantylmethyl)-2,4-dioxo-5-[3-hydroxy-2(R) aminopropyl]-2,3,4,5tetrahydro-1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea hydrochloride;

N-[1-(1-Cyclohexylmethyl-2,4-dioxo-5-[2-(diethylamino)ethyl]-2,3,4_l5-tetrahydro1 H-benzodiazepin-1 H-benzodiazepin-3-yl]-N’-phenylurea;

N-(1-(1-Adamantylmethyl)-2,4-dioxo-7-fluoro-5-[2-(4-morpholino)-ethyl]-2,3,4,5tetrahydro-1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

N-[1-(3-methyl-1-butyl)]-2,4-dioxo-5-(2-(4-morpholino)ethyl)]-2,3,4,5-tetrahydro30 1 H-benzodiazepin-3-yl]-N’-(4-chlorophenyl)urea;

N-[2,4-Dioxo-1-(3-methylbut-1-yl)-5-[2-(4-morpholino)ethyl]-2,3,4,5-tetrahydro1 H-1,5-benzodiazepin-3-yl]-N’-(4-trifluromethyl)phenylurea;

APiPJ 94/00657

BAD ORIGINAL &

1160CH

N-[1-(1-Adamantylmethyl)-2,4-dioxo-5-(2-(1-pyrrolidino)ethyl]-2,3,4,5-tetrahydro1H-benzodiazepin-3-yl]-N'-phenylurea

N-[2,4-Dioxo-1-[2-(hexamethyleneimino)ethyl]-5-phenyl-2_l3,4,5-tetrahydro-1 H5 benzodiazepin-3-yl}-N'-(3-tolyl)urea;

and enantiomers and physiologically acceptable salts thereof.

A particularly preferred compound of the invention is (-)[1-(1-Adamantylmethyl)]-2,4-dioxo-5-[2-(N-morpholino)-ethyl]-2,3,4,5tetrahydro-1 H-1,5-benzodiazepin-3-yl}-N'-phenylurea and physiologically acceptable salts thereof.

The pharmaceutically acceptable salts of the compounds of formula (I) include conventional salts formed for example from pharmaceutically acceptable inorganic or organic acids as well as quaternary ammonium acid addition salts. Examples of suitable salts include hydrochloric, hydrobromic, sulphuric, phosphoric, nitric, perchloric, fumaric, acetic, propionic, succinic, glycolic, formic, lactic, maleic, tartaric, citric, pamoic, malonic, hydroxymaleic, phenylacetic, glutamic, benzoic, salicylic, fumaric, toluenesulphonic, methanesulphonic, naphthalene-2-sulphonic, benzenesulphonic and the like. Other acids such as oxalic, while not in themselves pharmaceutically acceptable, may be useful in the preparation of salts useful as intermediates in obtaining the compounds of the invention and their pharmaceutically acceptable salts.

The compounds of formula (I) in which r5 represents hydrogen may form pharmaceutically acceptable salts with suitable cations. Suitable pharmaceutically acceptable cations include alkali metal (e g. sodium or potassium) and alkaline earth metal (e g calcium or magnesium) cations.

Salts may also be formed with organic bases e.g. N-methylglucamine.

The invention also includes metabolically labile esters of compounds of formula (I) wherein represents hydrogen. Examples of such metabolically labile esters include Chalky I esters e.g. methyl or ethyl esters, substituted or

APiPi 9 4 / 0 0 6 5 7

1160CH

AP.00492 unsubstituted aminoalkyl esters (e g. aminoethyl, 2-(N,N-diethylamino) ethyl, or 2-(4-morpholino)ethyl esters) or acyloxyalkyl esters such as, acyloxymethyl or 1 acyloxyethyl e.g. pivaloyloxymethyl, 1-pivaloyloxyethyl, acetoxymethyl, 1acetoxyethy 1, 1 -methoxy-1 -methy l-ethy Icarbonyloxy ethy 1,1 -benzoy loxyethy I, isopropoxycarbonyloxymethyl, 1-isopropoxycarbonyloxyethyl, cyclohexylcarbonyloxymethyl, 1-cyclohexylcarbonyloxyethyl ester, cyclohexyloxycarbonyloxymethyl, 1-cyclohexyloxycarbonyloxyethyl, 1-(4tetrahydropyranyloxycarbonyloxyethyl) or 1 -(4-tetrahydropyranylcarbonyloxy)ethyl.

C 10

The compound of formula (i) and salts and metabolically labile esters thereof may form solvates e g. hydrates and the invention includes such solvates.

The compounds of the invention are potent and specific antagonists of gastrin 15 and/or CCK and in particular gastrin and or CCK at the CCK-B-receptor. Thus compounds of the invention have been shown to be antagonists of CCK, particularly at CCK-B receptors as demonstrated for example by the compound’s ability to inhibit the contractile actions of CCK-4 in the presence of a CCK-A antagonist, in the guinea-pig isolated ileum longitudinal muscle20 myenteric plexus.

Compounds of the invention have also been found to have a significantly weaker activity at CCK-A receptors compared with their activity at gastrin and/or CCK-B receptors, as demonstrated by their ability to inhibit the contractile activity of CCK-8 in guinea-pig isolated ileum longitudinal muscle-myenteric plexus.

The preparation and use of guinea-pig isolated ileum longitudinal musclemyenteric plexus has been described by K-H Buchheit et al in Nauyn30 Schmeideberg's Arch. Pharmacol, (1985), 329, p36-41 and by V.L. Lucaites et al (1991) in J. Pharmacol. Exp. Ther., 256, 695-703.

The compounds of the invention have also been shown to be antagonists of gastrin as demonstrated by their ability to inhibit pentagastrin-stimulated acid

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BAD ORIGINAL ft secretion from rat isolated gastric mucosa using the procedure described by J J Reeves and R. Stables in Br. J, Pharmac., 1985, 86, p.677-684.

The greater affinity of the compounds of the invention for the CCK-B receptor 5 over the CCK-A receptor has also been established using the CCK receptor binding assays described by G Dal Fomo et al., J. Pharmcol. Exp & Ther. 261.

1056-1063,1992.

The compounds of the invention are therefore useful for the treatment and/or 10 prevention of disorders in mammals, especially humans, where modification of the effects of gastrin or CCK is of therapeutic benefit. Thus the compounds of the invention are useful for the treatment of central nervous system disorders where CCK and/or gastrin are involved. For example anxiety disorders (including panic disorder, agoraphobia, social phobia, simple phobia, obsessive 15 compulsive disorders, post traumatic stress disorder, and general anxiety disorder), tardive dyskinesia, depression, Parkinson's disease or psychosis.

The compounds of the invention are also useful for the treatment of gastrointestinal disorders especially those where there is an advantage in lowering gastric acidity. Such disorders include peptic ulceration, reflux 20 oesophagitis and Zollinger Ellison syndrome. They may also be useful for the treatment of gastrointestinal disorders such as irritable bowel syndrome, excess pancreatic secretion, acute pancreatitis, motility disorders, antral G cell hyperplasia, fundic mucosal hyperplasia or gastrointestinal neoplasms. They may also be useful for the treatment of dependency on drugs or substances of 25 abuse and withdrawal, Gilles de la Tourette syndrome, or dysfunction of appetite regulatory systems; as well as the treatment of certain tumours of the lung, lower oesophagus, pancreas, stomach, intestines and colon. Compounds of the invention are also useful for directly inducing analgesia, or enhancing opiate or non-opiate mediated analgesia, as well as anaesthesia or loss of the 30 sensation of pain.

Compounds of the invention have also been found to exhibt anxiolytic activity in conventional pharmacological tests. For example in mice in the black-white box test and marmoset threat test_

1160CH

AP.00492

The invention therefore provides a compound of formula (I) or a pharmaceutically acceptable salt or solvate thereof for use in therapy, in particular in human medicine.

According to another aspect the invention provides the use of a compound of formula (I) or a pharmaceutically acceptable salt or solvate thereof for the manufacture of a medicament for the treatment of conditions where modification of the effects of gastrin and/or CCK is of therapeutic benefit.

f 10 According to a further aspect of the invention we provide a method for the treatment of a mammal, including man, in particular in the treatment of conditions where modification of the effects of gastrin and/or CCK is of therapeutic benefit which method comprises administering an effective amount of a compound of formula (I) or a pharmaceutically acceptable salt or solvate thereof to the patient.

It will be appreciated by those skilled in the art that reference herein to treatment extends to prophylaxis as well as the treatment of established diseases or symptoms.

It will further be appreciated that the amount of a compound of the invention required for use in treatment will vary with the nature of the condition being ^f treated and the age and the condition of the patient and will be ultimately at the discretion of the attendant physician or veterinarian. In general however doses employed for adult human treatment will typically be in the range of 0.01 2000mg per day e g 0.01-500mg per day.

The desired dose may conveniently be presented in a single dose or as divided doses administered at appropriate intervals, for example as two, three, four or more sub-doses per day.

Because the compounds of the invention antagonise the function of CCK in animals, they may also be used as feed additives to increase the food intake in animals in daily dosages of around 1 mg/kg to 10mg/kg.

AP/P/ 9 4 / 0 0 6 5 7

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1160CH

While it is possible that, for use in therapy, a compound of the invention may be administered as the raw chemical it is preferable to present the active ingredient as a pharmaceutical formulation.

r

The invention thus further provides a pharmaceutical formulation comprising a compound of formula (I) or a pharmaceutically acceptable salt thereof together with one or more pharmaceutically acceptable carriers therefor and, optionally, other therapeutic and/or prophylactic ingredients. The carrier(s) must be 'acceptable' in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.

The compositions of the invention include those in a form especially formulated for oral, buccal, parenteral, implant, or rectal administration. Oral administration is preferred.

Tablets and capsules for oral administration may contain conventional excipients such as binding agents, for example, syrup, accacia, gelatin, sorbitol, tragacanth, hydroxypropyl cellulose, mucilage of starch or polyvinylpyrrolidone, fillers, for example, lactose, sugar, microcrystalline cellulose, maize-starch, calcium phosphate or sorbitol; lubricants, for example, hydrogenated vegetable oils, magnesium stearate, stearic acid, talc, polyethylene glycol or silica; disintegrants, for example, potato starch or sodium starch glycollate, or wetting agents such as sodium lauryl sulphate. The tablets may be coated according to methods well known in the art. Oral liquid preparations may be in the form of, for example, aqueous or oily suspensions, solutions emulsions, syrups or elixirs, or may be presented as oral drops or a dry product for constitution with water or other suitable vehicle before use. Such preparations may contain conventional additives such as suspending agents, for example, sorbitol syrup, methyl cellulose, glucose/sugar syrup, gelatin, hydroxyethylcellulose, carboxymethyl cellulose, aluminium stearate gel or hydrogenated edible fats; emulsifying agents, for example, lecithin, sorbitan mono-oleate or acacia; non-aqueous vehicles (which may include edible oils), for example, almond oil, fractionated coconut oil, oily esters, propylene glycol or ethyl alcohol; and preservatives, for example, methyl or propyl β-hydroxybenzoates or sorbic acid. The

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AP .0 0 4 9 2 compositions may also be formulated as suppositories, eg. containing conventional suppository bases such as cocoa butter or other glycerides.

For buccal administration the composition may take the form of tablets or 5 lozenges formulated in conventional manner.

The composition according to the invention may be formulated for parenteral administration by injection or continuous infusion. Formulations for injection may be presented in unit dose form in prefilled syringes, vials and ampoules, or f 10 in multi-dose containers with an added preservative. The compositions may take such forms as suspensions, solutions, or emulsions in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising and/or dispersing agents. Alternatively the active ingredient may be in powder form which may be obtained by freeze drying for constitution with a suitable vehicle, e.g. sterile, pyrogen-free water, before use.

The composition according to the invention may also be formulated as a depot preparation. Such long acting formulations may be administered by implantation (for example subcutaneously or intramuscularly) or by intramuscular injection. Thus for example, the compounds of the invention may be formulated with suitable polymeric or hydrophobic materials (for example as an emulsion in an acceptable oil) or ion exchange resins, or as sparingly soluble derivatives, for example, as a sparingly soluble salt.

The compositions according to the invention may contain between 0.1 - 99% of the active ingredient, conveniently from 30-95% for tablets and capsules and 350% for liquid preparations.

Compounds of general formula (I) and salts thereof may be prepared by the general methods outlined hereinafter. In the following description, the groups R¹-R1O are as defined for the compounds of formula (I) unless otherwise stated.

According to a first general process (A) compounds of formula (I) wherein X is

94/00657

ΓιΟ/Μ I ι ιου^π represents the group NHCOR'¹ wherein R¹¹ is an optionally substituted phenoxy group or a 1-imidazole group.

H₂NR² (III) optionally in the presence of a base such as a tertiary amine (e.g.

triethylamine). The reaction conveniently takes place in a suitable solvent such as a halogenated hydrocarbon (e.g. dichloromethane) or an ether (e.g. tetrahydrofuran) or an amide e.g. N,N- dimethylformamide optionally at a temperature ranging from room temperature to the reflux temperature of the solvent.

In a particular aspect of the process (A) when Y is the group NHCOR¹¹ and R¹¹ is a 1-imidazole group, the imidazolide (II) may be formed in situ in which case the amine of formula (III) will be mixed with a compound of formula (IV)

AP/P/ 9 4 / 0 0 6 5 7

in the presence of carbonyldiimidazole under the aforementioned conditions. For process A when Y is the group NHCOR^{1 1} and R¹ 1 is optionally substituted phenoxy group the reaction with the primary amine (III) is preferably carried out in the presence of a base such as a tertiary amine e g. triethylamine.

1160CH

AP.00492

The compounds of formula (II) wherein R¹¹ is an optionally substituted phenoxy group may be prepared from the primary amine (IV) by reaction with the corresponding optionally substituted phenyl chloroformate in the presence of a base such as pyridine. The reaction may be carried out in a solvent such as a halohydrocarbon e.g. dichloromethane and at a temperature from 0-50θ.

Compounds of formula (II) wherein R**¹ is a 1-imidazole group may be prepared by reacting a compound of formula (IV) with carbonyldiimidazole in the presence of a suitable solvent such as a halogenated hydrocarbon (e g. dichloromethane) or an ether (e g. tetrahydrofuran) at a temperature ranging from 0° to 80θ (conveniently at room temperature).

According to a further general process (B) compounds of formula (I) may be prepared by reacting a compound of formula (IV) with an isocyanate of formula (V)

O=C=N-R2 (V) or an acyl chloride of formula (VI) ²⁰

CICO(X)R² (VI)

The reaction conveniently takes place in the presence of a suitable solvent such as a halohydrocarbon (e.g. dichloromethane), an ether (e.g tetrahydrofuran) or a nitrile (e.g. acetonitrile) or a mixture thereof at a temperature in the range of 0°C to 80°C.

AP/P/ 9 4 / 0 0 6 57

Compounds of formula (IV) may be prepared by reduction of compounds of formula (VII)

1160CH wherein R³a represents the group R³ as defined in formula (I)

Compounds of formula (VII) may be reduced to a compound of formula (IV) by 5 reaction with zinc and acetic acid. This reaction may be carried out a temperature with the range 0-50°. Alternatively the reduction may be carried out using palladium on charcoal and ammonium formate in a solvent such as methanol.

Compounds of formula (VII) may be prepared by reaction of the orthophenylenediamine (VIII) with the diacid chloride (IX), in a suitable solvent such as an ether e.g. tetrahydorfuran or an ester e g. ethyl acetate.

CIOC

CIOC

NNHPh (IX)

Compounds of formula (VIII) are either known compounds or may be prepared by analogous methods. Thus for example a compound of formula (VIII) may be prepared by alkylation of the amine (X).

ZS900M6 /d'dV

Thus the amine (X) may be reacted with the compound R¹ L, in which L is a leaving group e.g. chlorine or bromine, optionally in the presence of sodium iodide in a solvent such as Ν,Ν-dimethylformamide. Alternatively the group R¹ may be introduced wherein the amine (X) is reacted with an appropriate

1160CH

AP.00492

In general, the compounds of formula (III), V and (VI) are either known compounds or may be prepared according to methods used for the preparation of known compounds,

According to a further process (C) a compound of formula (I) may be prepared by reaction of a compound of formula (XI)

with reaction with the alkylating agent R⁸R⁹N Aik L wherein L is a leaving group e.g. a halogen. This process is conveniently carried out in the presence of a strong base such as an alkali metal carbonate e.g. potassium carbonate or sodium hydride and in an aprotic solvent such as NN-dimethyl formamide.

The compounds of formula (XI) may be prepared from the amine (XII) wherein R¹, R¹⁰ and m have the meanings defined on formula (I) and R³a is a hydrogen atom or a nitrogen protecting group.

AP/P/ 9 4 / 0 0 6 5 7 by reaction with the isocyanate (V) or the acid chloride (VI) under the conditions described above for the preparation of the compounds of formula (I) from the amine (IV), followed if necessary by removal of the nitrogen protecting group R³a

Compounds of formula (XII) may be prepared from compounds of formula (VII) wherein R³a is nitrogen protecting group e.g. benzyl or p-methoxybenzyl

1160CH group. Thus the compound of formula (VII) may be reduced with palladium on charcoal in the presence of ammonium formate to give the required compound of formula (XII) wherein R³a represents a hydrogen atom. Alternatively the required compound of formula (XII) may be prepared by reduction of the compound of formula (VII) wherein R³a is a nitrogen protecting group followed if desired by the subsequent removal of the said R³a nitrogen protecting group using conventional procedures. Thus the reduction of the hydrazone may be affected using zinc and acetic acid and if desired, the nitrogen protecting group subsequently removed by hydrogenolysis or reaction with ceric ammonium nitrate.

The amines of formula (VIII wherein R³a is hydrogen) or formula (XI) are either known compounds or may be prepared from the corresponding nitro derivatives (XIII) or (XIV)

Reaction of the nitro compounds (XIII) or (XIV) with Na₂S₂O₄ yields the corresponding primary amine which can then be alkylated in a conventional manner to give the required diamine (VIII).

AP/P/ 9 4 / 0 0 6 5 7

The compounds of formula (XII) wherein R³a is hydrogen may be prepared from the compounds of formula (XV)

NHOCOCHjPh (XV) by removal of the N-benzyloxycarbonyl protecting group using standard procedures e.g. hydrogenolysis with hydrogen and a palladium catalyst.

1160CH

AP.00492

The compounds of formula (XV) may be prepared by treating the compound (XVI)

COjC^

NHCO^CH^

COCHNHOCOCH^h with a suitable acid e.g. hydrochloric acid.

The compound of formula (XVI) may be prepared by reaction of the protected 10 diamine (XVII)

HO₂C-CHCO₂C₂H5

NHCO-jCiCH^j (XVII)

NHOCOCHjPh (XVIII) with the acid (XVIII) in the presence of dicyclohexylcarbodimide.

The protected diamine (XVII) may be prepared from the corresponding nitro 15 derivative (VIII) by standard procedures. Thus the nitro group may be reduced by reaciton with Na2S2O4 and the resultant amine converted into the corresponding N-t-butoxycarboxy derivative by standard procedures.

According to a further process (D) compounds of formula (I) may be prepared by 20 reaction of a compound of formula (XIX), wherein R¹, R², R¹⁰, m and alk have the meanings defined in formula (I) and L is a leaving group

AP/P/ 9 4 / 0 0 6 5 7

BAD ORIGINAL- £,

1160CH

with an amine R⁸a R% NH wherein R⁸a and or R⁹b NH wherein R⁸a and or R⁹b, have the meanings defined for the groups R⁸ and R⁹ respectively or each may independently represent a nitrogen protecting group such as an arylmethyl group e.g. benzyl, or p-methoxybenzyl, followed where necessary or desired by removal of any said nitrogen protecting group. The reaction may be carried out in the absence or presence of and additional solvent e g. an ether such as tetrahydrofuran. Conveniently the leaving group L is a halogen atom of e.g. chlorine bromine or iodine, or sulphonyloxy such as alkylsulphonyloxy e.g. methanesulphonyloxy or arylsulphonyloxy e.g. phenylsulphonyloxy. The nitrogen protecting group R⁸a or R⁹b may be removed by conventional procedures e.g. hydrogenolysis.

The compounds of formula (XIX) may be prepared from the corresponding compound of formula (XIX) wherein L represents an hydroxyl group using conventional procedures. Thus compounds of formula (XIX) wherein L is a sulphonyloxy group may be prepared by reaction of the corresponding hydroxy compound with the appropriate alkyl or aryl sulphonylchloride in the presence of a tertiary organic base.

Compounds of formula (XIX) wherein L is a halogen atom may be prepared from the corresponding hydroxy compound using standard procedures for converting hydroxyl groups into halo derivatives. Thus for example compounds of formula (XIX) wherein L is bromine may be prepared by treatment of the corresponding hydroxyl compound with carbon tetrabromide and triphenyl phosphine.

AP/P/ 9 4 / 0 0 6 5 7

Compounds of formula (XIX) wherein L is hydroxy may be prepared from the corresponding amine (VIII) wherein R³a is a protected hydroxyalkyl group by reaction with the diacid chloride (IX) followed by reduction of the hydrazone thus

1160CH

AP . Ο Ο 4 9 2

94/00657 formed to give the amine (XII) wherein R³a is an hydroxyalkyl group or a protected derivative thereof.

Reaction of the resultant amine (XII) with the isocyanate (V) or acyl chloride (VI) yields the desired compound XIX wherein L is an hydroxyl group.

According to a further process (E) a compound of formula (I) may be converted into another compound of formula (I) using conventional techniques.

Thus compounds of formula (I) wherein r2 is phenyl substituted by hydroxy may be prepared from compounds wherein r2 is phenyl substituted by methoxy by conventional means e.g. reaction with aluminium iodide. Similarly compounds of formula (I) wherein R2 is a phenyl group substituted by a carboxyl group may be prepared by hydrolysis of the corresponding compound of formula (I) wherein

R2 is a phenyl group substituted by an alkoxycarbonyl group.

In the processes described above the groups R¹, r2 and R³ in the intermediates II, III, V and VI may be a group as defined in formula (I) or a group convertible thereto.

The metabolically labile esters of the compounds of formula (I) may be prepared from the corresponding carboxylic and by conventional means.

Acid addition salts of compounds of formula (I) may be prepared by jT conventional means. Thus for example a compound of formula (I) may be 25 treated with the desired acid, conveniently in the presence of a solvent, e.g. an alkanol to give a solution of the required salt which may then be isolated in a conventional manner.

Compounds of formula (I) contain at least one asymmetric carbon atom, namely the carbon atom of the diazepine ring to which the grouping NHCOXR² is attached. Specific enantiomers of the compounds of formula (I) may be obtained by resolution of the racemic compound using conventional procedures such as chiral HPLC. Alternatively the specific enantiomers of formula (I) may be prepared from the appropriate enantiomer of the compounds of formula (IV)

1160CH using the processes described above for preparing compounds as the invention from the compounds of formula (IV)

The specific enantiomers of the compound of formula (IV) may be prepared by 5 conventional procedures. Thus the racemic amine (IV) may be reacted with an optically active reagent such as a derivative of phenylalanine or Mandelic acid and the resultant diastereoisomers may be separated by conventional procedures. The required enantiomeric amine (IV) may then be obtained from the single diastereoisomer by removal of the phenylanine or mandelic acid residue by conventional procedures.

The following examples, which are non-limiting, illustrate the invention.

In the Preparations and Examples, unless otherwise stated; Melting points (m.p.) were determined on a Buchi m.p. apparatus and are uncorrected. All

Otemperatures refer to OC. Infrared spectra were measured in chloroform-dj solutions on a FT-IR instrument. Proton Magnetic Resonance (1H-NMR) spectra were recorded at 300MHz as solutions in chloroform-d-|. Chemical shifts are reported in ppm downfield (d) from Me4Si as an internal standard, and are assigned as singlets (s), doublets (d), doublet of doublets (dd) or multiplets (m). Column chromatography was carried out over silica gel (Merck AG Darmstadt, Germany). Solutions were dried over anhydrous sodium sulphate. Petrol refers to petroleum ether,b.p.40-600c. Dichloromethane was redistilled over calcium hydride; tetrahydrofuran was redistilled over sodium; ethyl ether was redistilled over sodium and ethyl acetate was dried over activated molecular sieves. The following abbreviations are used in the text. EA = ethyl acetate, CH = cyclohexane, P = petroleum ether 40-60°C, THF = tetrahydrofuran, DCM = dichloromethane, EE = ethyl ether, DMF = Ν,Ν-dimethylformamide. Tic refers to thin layer chromatography on silica plates. All the compounds are intended as racemic mixtures unless otherwise indicated.

AP/P/ 9 4 / 0 0 6 5 7

Intermediate 1

N-(4-Methoxyphenylmethvl)-2-nitroaniline

A mixture of 1-fluoro-2-nitrobenzene (20g) and 4-methoxybenzylamine

1160CH nitrogen atmosphere. The mixture was filtered, then the organic layer was concentrated in vacuo to an oil. Ethanol (50ml) was added and a solid separated. After filtration, the title compound was obtained as an orange solid (16.35g). The filtrate was concentrated in vacuo and the residue was treated with further ethanol (10ml) to give a further amount of title compound (7.9a).

M.p.81-2O T.I.c. CH-EA (8:2), Rf0.55.

Intermediate 2

N-(4-Methoxyphenylmethvl)-1,2-phenylenediamine

Potassium carbonate (96.95g) and sodium hydrosulfite (80.96g) were added to a suspension of the intermediate 1 (24g) in 95% ethanol (500ml) and water (500ml). The mixture was stirred at 230 for 1 h, then acidified with conc.hydrochloric acid (150ml) until pH=3. The mixture was concentrated in vacuo to half volume and the residue was basified with a 10% sodium hydroxide solution (900ml) until pH=10. The mixture was extracted with ethyl acetate (1200ml). The organic layer was washed with brine (600ml), dried, and concentrated in vacuo to a brown solid. This material was triturated with diethyl ether to give the title compound as a beige solid (17.7g). Μ.ρ.91-2θ T.I.c. CHEA (8:2), Rf 0.22.

Intermediate 3

N-(4-Methoxyphenylmethvl)-N'-(3-methvl-1-butvl)-1,2-phenylenediamine

Bromo 3-methylbutane (10.68ml) was added to a solution of intermediate 2 (19.5g) and sodium iodide (12.8g) in dimethylformamide (400ml) under a nitrogen atmosphere. The solution was heated to 80° for 4h under a nitrogen atmosphere, then cooled to room temperature, diluted with water (300ml) and extracted with diethyl ether (2x700ml). The combined organic extracts were washed with brine (1000ml), dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with CH-EA 9:1) to give the title compound as a yellow oil (14.0g). T.I.c. CH-EA (9:1), Rf 0.42. IR : 1610 and 1601 (C=C) cm-1; 1H-NMR :7.31 (d); 6.89 (d); 6.84-6.74 (m); 4.22 (s); 3.81 (s); 3.10 (t); 1.75 (m); 1.6-1.5 (m); 0.94 (d).

Intermediate 4

AP/P/ 94/00657

BAD ORIGINAL

1160CH

2,4-Dioxo-5-(4-methoxvphenvlmethvl)-1-(3-methvl-1-butyl)-3-phenvlhvdrazono2,3,4.5-tetrahvdro-1 H-1.5-benzodiazepine

Intermediate 3 (14.0g) and 2-phenylhydrazonomalonyldichloride (13.8g) were each taken up in THF (100ml) and dropped in a flask containing THF (100ml) under a nitrogen atmosphere. After complete addition, the solution was heated at 500 for 2h. The solution was concentrated in vacuo and the residue was triturated with diethyl ether to give the title compound as a yellow solid (8.9g). The filtrate was concentrated in vacuo and purified by flash chromatography (eluting with CH-EA 8:2) to give a further amount of title compound (6.35g).

< 10 M.p.189-1910 T.I.c. CH-EA (8:2), Rf 0.30.

Intermediate 5

3-Amino-2.4-dioxo-5-(4-methoxyphenvlmethvl)-1-(3-methvl-1-butvl)-2,3.4,5tetrahvdro-1 H-1.5-benzodiazepine

Zinc dust (15.8g) was added to a suspension of the intermediate 4 (15.25g) in acetic acid (150ml). The mixture was heated at 400 for 2h, then decanted from zinc. The filtrate was basified with a 10% sodium hydroxide solution until pH=10 (2000ml) and the mixture extracted with ethyl acetate (2000ml). The combined organic extracts were washed with brine (1000ml), dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with EA) to give the title compound as a white solid (8.24g). M.p. 115-60 T.I.c. EA-MeOH (95:5), Rf 0.25.

z f

Intermediate 6

3-Amino-2.4-dioxo-1 -(3-methyl-1 -butvl)-2.3.4,5-tetrahvdro-1 H-1,5benzodiazeoine

Ammonium cerium (IV) nitrate (17.45g) was added to a solution of the intermediate 5 (3.0g) in acetonitrile (90ml) and water (10ml). The solution was stirred at 23° for 36h,then concentrated in vacuo to a slurry solid. This material was diluted with a 10% sodium hydroxide solution (150ml), stirred at 23© for 30min, then inorganic salts were filtered off. The aqueous solution was extracted with ethyl acetate (4x100ml). The combined organic extracts were washed with brine (300ml),dried and concentrated in vacuo to give an oil, that was purified by flash chromatography (eluting with DCM-MeOH 95.5) to give the

AP/P/ 9 4 / 0 0 6 5 7

1160CH

AP, Ο Ο 4 9 2 title compound as a white solid (0.6g). M.p. 148-1500 T.I.c. DCM-MeOH (95:5), Rf 0.20.

Intermediate 7

N-f2.4-dioxo-1 -(3-methvl-1 -butvl)-2.3.4.5-tetrahvdro-1 H-1.5-benzodiazepin-3-vnN'-phenvIurea

Phenyl isocyanate (0.183ml) was added to a solution of the intermediate 6 (0.4g) in dry dichloromethane (5ml) under a nitrogen atmosphere. The mixture was stirred at 230 for 30min, then concentrated in vacuo. The residue was triturated with diethyl ether to give the title compound as a white solid (0.478g). M.p. 249-2500. T.I.c. DCM-MeOH (95:5), Rf 0.38.

Intermediate 8

N-f2.4-dioxo-1 -(3-methvl-1-butyl)-2,3.4,5-tetrahvdro-1 H-1.5-benzodiazepin-3-vn15 N'-(4-methoxyphenyl)urea

4-Methoxyphenyl isocyanate (0.1ml) was added to a solution of the intermediate 6 (0.19g) in dry dichloromethane (5ml) under a nitrogen atmosphere. The mixture was stirred at 23° for 30min, then concentrated in vacuo. The residue was triturated with diethyl ether to give the title compound as a white solid (0.197g). M.p. 150-1520. T.I.c. EA-MeOH (95:5), Rf 0.78.

Intermediate 9

N-i2.4-Dioxo-5-(4-methoxvphenvlmethvl)-1-(3-methvl-1-butyl)-2.3,4.5tetrahvdro-1 H-1 .S-benzodiazepin-S-vll-N'-phenvIurea 25 Phenyl isocyanate (0.017ml) was added to a solution of the intermediate 5 (0.05g) in acetonitrile (1ml). The mixture was stirred at 23° for 30min, then filtered to give the title compound as a white solid (0.062g). M.p. 206-80. T.I.c. CH-EA(1:1), Rf0.4.

Intermediate 10

-(3-methvl-1 -butyl) amino-2-nitrobenzene

A solution of amino 3-methylbutane (1,5g) in THF (20ml) was dropped into a solution of 2-fluoronitrobenzene (2.4g) in THF (20ml), at 23° under a nitrogen atmosphere. The mixture was stirred at 23* for 3h, then heated at reflux for 1.5h.

The mixture was allowed to cool to 23’, then concentrated under vacuum to give

AP/P/ 9 4 / 0 0 6 5 7

1160CH a crude compound which was purified by flash chromatography on silica gel using CH-EA 9/1 as eluants to give the title compound as a yellow oil (2.12g). T.I.c. CH-EA ( 8/2 ), Rf 0.79 . IR :3383 (NH); 1620 (C=C) cm'¹

Intermediate 11

2-(3-methyl-1-butyl) amino-aniline

A solution of potassium carbonate (9.1g) and sodium hydrosulfite (8 g) in water (50ml) was added to a mixture of intermediate 10 (2.12g) in ethanol (30ml) and water (70ml). The mixture was stirred at 23° for 1 h, then acidified with cone, hydrochloric acid until pH=3. The mixture was then basified with a 10% sodium hydroxide solution until pH=10 and extracted with ethyl acetate (2x100ml); the combined extracts were washed with brine (150ml),dried and concentrated in vacuo to give the title compound as a brown solid (1.8g). T.I.c. CH-EA (8/2 ), Rf 0.36 . IR :3420 (NH); 1620 (C=C) cm’¹

Intermediate 12

N-(2,2-dimethylethoxvcarbonvl)-N'-(3-methvl-1-butyl )-1.2-phenylenediamine

Di-t-butyl dicarbonate (2.44g) and sodium hydrogen carbonate (1,42g) were added to the solution of intermediate 11 ( 3g ) in THF (50ml) /water (40ml); the mixture was stirred at 30° for 1,5h and concentrated in vacuo. The residue was diluted with ethyl acetate (150ml) and washed with water(50ml) and brine (50ml). The organic layer was dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with CH/EA 9/1 ) to give the title compound as a wax (3,1 g). T.I.c. CH-EA (9:1), Rf=0.37. IR :3420 (NH); 17221697 (C=O) cm'¹

Intermediate 13

N-(2.2-dimethvlethoxycarbonvl) -N'-[2-( 1 -benzvloxvcarbonvlamino-1 ethoxvcarbonvl)-2-oxo ethyll-N'-O-methvl-l -butvl)1,2-phenvlenediamine

To a solution of benzyloxycarbonylamino malonic acid monoethyl ester (0.90g) in ethyl acetate (40 ml), Ν,Ν'-Dicyclohexycarbodiimide (0.76 g) and 1Hydroxybenzotriazole hydrate (0.55 g) were added. After complete addition the mixture was stirred at 20° for 1h, then a solution of intermediate 12 ( 0.88 g ) in ethyl acetate (20ml) was added and stirring was continued for 2 h. The reaction mixture was then heated at reflux for 4h and left at 20° for 20h , filtered, and

AP/P/ 94/00657

1160CH

AP. Ο Ο 4 9 2 washed with water (50ml) and brine (50ml). The organic layer was dried, concentrated in vacuo and the residue was purified by flash chromatography (eluting with CH/EA 9/1 ) to give the title compound as an oil (0.64g). T.I.c. CH-EA (8:2), Rf 0.33. IR :3500-3300 (NH); 1726-1672 (C=O) cnr¹

Intermediate 14

1-i3-methvl-1-butvl)-3-benzvloxvcarbonylamino-2.4-dioxo-2.3.4.5-tetrahvdro1H-1.5-benzodiazepine

Concentrated hydrocloric acid (5ml) was added to a suspension of intermediate

13 (0.64g) in ethanol (15 ml). The mixture was stirred at 23® for 2h, diluted with ethyl acetate, washed with water,dried and concentrated in vacuo to an oil (

0.49 g ), which was purified by flash chromatography (eluting with to give the title compound as a white foam (0.23g). T.I.c. EA-CH 1:1, Rf 0.59. IR :3431,3256 (NH); 1734,1717 (C=O) cm-¹

Intermediate 15

5-f2-(diethylamino)ethvH-1-(3-methyl-1-butyl)-3-benzvloxvcarbonvlamino-2.4dioxo-2,3.4.5-tetrahvdro-1 H-1,5-benzodiazepine A solution of intermediate 14 (0.22g) and potassium carbonate (0.24g) in acetone ( 30 ml)/ water (1 ml) was stirred at reflux for 6h. The solution was concentrated in vacuo, the residue was diluted with ethyl acetate (150ml) and washed with water (50ml) and brine (50ml). The organic layer was dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with EA-MeOH 9:1) to give the title compound as an oil (0.194g). T.I.c.

EA-CH (3:1), Rf=0.23. IR :3400 (NH); 1697-1668 (C=O) cm-¹

Intermediate 16

3-amino-5-f2-(diethvlamino)ethvl1-1-(3-methyl-1-butvl)-2.4-dioxo-2.3.4.5tetrahydro-1 H-1.5-benzodiazepine

5% Pd/C (0.04 mg) was added to a solution of intermediate 15 (0.18g) in methanol (20ml) and the mixture was hydrogenated at 1 atm. for 2h. The catalyst was filtered over celite and the filtrate concentrated in vacuo and the residue was dissolved in ethyl acetate (100ml), washed with water( 100ml), brine (10ml), dried and concentrated in vacuo to give the title compound as a

AP/P/ 94/00657

1160CH

Intermediate 17

N-( 1-Adamantylcarbonvl)-N'-(4-Methoxypheny Imethyl )-1,2-phenvlenediamine

-Adamantanecarbonyl chloride (1.91 g ) ) was dropped into a solution of intermediate 2 (1.83g ) and triethylamine (1.45 ml ) in dry THF (100ml at23°, under a nitrogen atmosphere. The mixture was stirred at reflux for 3h, allowed to cool to 20°, then diluted with ethyl acetate (120ml) washed with brine (150ml), dried, and concentrated in vacuo . This material ( 3.21 g) was crystallised from DCM/CH to give the title compound as a white solid (2.3g). T.I.c. CH-EA (8:2),

Rf 0.34 IR 3393, 3304 (NH ) cm-1 ·

Intermediate 18

N-( 1 -Adamantvlmethvl)-N^,-(4-Methoxyphenvlmethvl)-1.2-phenvlenediamine

Borane dimethylsulfide complex (10 M solution; 15ml) was added dropwise, under a nitrogen atmosphere, to a solution of intermediate 17 (2.3g) in dry THF ( 70ml ) previously heated at reflux. Dimethyl sulfide and THF ( 50ml) were distilled and the solution was allowed to cool to r.t., then a 10% potassium carbonate solution (30ml ) was added and the mixture was stirred at 20° for 40 min.Then it was diluted with methanol ( 20 ml ) and strirred at reflux for 3h, then at 20° for 20h. ethyl acetate (100 ml) was added;the layers were separated the organic extracts washed with brine (2x50 ml ), dried and concentrated in vacuo to an oil ( 0.20g), which was purified by flash chromatography (eluting with CH-EA 9:1) to give the title compound (0.2g)as a white solid, m.p 132134. T.I.c. CH-EA (9:1), Rf 0.63.

AP/P/ 94/00657

Intermediate 19

1-(1 -Adamantvlmethyl)-dioxo-5-(4-methoxvphenvlmethvl)-3-phenvlhvdrazono2.3.4.5-tetrahvdro-1 H-1.5-benzodiazepine

Intermediate 18 (1.22g) and 2-phenylhydrazonomalonyldichloride (0.940g) were each taken up in THF (40ml) and dropped in a flask containing THF (40ml) under a nitrogen atmosphere. After complete addition, the solution was heated at 500 for 4h. The solution was diluted with ethyl acetate (100ml) and washed with saturated sodium hydrogen carbonate ( 2x100 ml) and brine ( 2x80 ml) .dried and concentrated in vacuo to give the title compound as a yellow foam

AP.00492 (1.64g). M.p. 170-880 T i c. CH-EA(8:2), Rf 0.60. IR .3441 (NH); 1661 , 1653 (C=O) cm-1;

Intermediate 20

1-(1 -Adamantvlmethvl)-3-amino-2.4-dioxo-5-(4-methoxyphenvlmethvl)-2.3.4,5tetrahvdro-1 H-1.5-benzodiazepine

Zinc dust (1.46g) was added to a suspension of the intermediate 19 (1.36g) in acetic acid (30ml). The mixture was stirred at 20“for 18h, then a further amount of Zinc dust (0.30g) in acetic acid (3ml) was added a stirring continued for Ih.The mixture was decanted from zinc, the filtrate diluted with ethyl acetate (150ml), washed with saturated sodium hydrogen carbonate (2x150 ml) and brine ( 200 ml), dried and concentrated in vacuo to an oil (1.31g), which was purified by flash chromatography (eluting with EA, then EA/Methanol 95/5) to give the title compound as a pale yellow solid (0.90g). M.p.223-5o T.I.c. DCMMeOH (95:5), Rf 0.34. IR : 1700 and 1670 (C=O) cm-1;

Intermediate 21

-(1 -Adamantylmethyl)-3-amino-2,4-dioxo-2.3.4.5-tetrahydro-1 H-1,5benzodiazepine

Ammonium cerium (IV) nitrate (3.85g) was added to a solution of the intermediate 20 (0.80g) in acetonitrile (125ml) and water (5ml). The solution was stirred at 23° for 36h,then water ( 5ml) was added to the suspension and stirring continued for 30h at 50° The solution was concentrated in vacuo to a slurry solid. This material was diluted with a 10% sodium hydroxide solution (250ml) and stirred at 230 for 1 h, then inorganic salts were filtered off. The aqueous solution was extracted with ethyl acetate (2x150ml), the combined organic extracts were washed with brine (300ml),dried and concentrated in vacuo to give an oil (0.750g), that was purified by flash chromatography (eluting with DCM-MeOH 95:5) to give the title compound as a white solid (0.500g). T.I.c. DCM-MeOH (95:5), Rf 0.34. IR : 3213-3126 (NH and NH2), 1705,1668 and 1660 (C=0)cm-1;

APIP! 9 4 / 0 0 6 57

Intermediate 22

N-f(1 -Adamantvlmethvl)-2.4-dioxo-2,3.4.5-tetrahvdro-1 H-1.5-benzodiazepin-3vll-N'-phenylurea

BAD ORIGINAL Q

1160CH

Phenyl isocyanate (0.05ml) was added to a solution of the intermediate 21 (0.14g) in dry acetonitrile (10ml) under a nitrogen atmosphere. The mixture was stirred at 23θ for 30min, then the solid was filteredoff, washed with acetonitrile to give the title compound as a white solid (0.146g). M.p.> 280°. T.I.c. DCM5 MeOH (95:5), Rf 0.46. IR : 3383, 3215 (NH), 1697 and 1676 and 1665 (C=O); 1597 (C=C) cm-1

Intermediate 23

141 -Adamantvlcarbonvlamino)-2-nitrobenzene

A solution of 1 -adamantanecarbonyl chloride (17.95g) in acetone (60ml) was dropped into a solution of 2-nitroaniline (10.4g) and triethylamine (12.6ml) in acetone (50ml), at⁰ under a nitrogen atmosphere. The mixture was stirred at 23° for 22h, then further acetone (50ml) was added.The mixture was heated at 70° for 3h. The mixture was allowed to cool to 23°, then filtered; the brown solid obtained was crystallized from acetone to give the title compound as a yellow solid (17.3g). T.I.c. CH-EA(10;2), Rf0.67. M.p. 111-4°.

Intermediate 24

1- (1 -Adamantylmethylamino)-2-nitrobenzene

Borane dimethylsulfide complex (10M solution; 6.0ml) was added, dropwise, under a nitrogen atmosphere, to a solution of intermediate 23 (13.5g) in dry toluene (160ml) previously cooled to 10° . The solution was stirred at 10° for 15min, then heated at 110° for 1h. The solution was allowed to cool to room temperature, then a 10% potassium carbonate solution (50ml) was added and the mixture was stirred at 23°for 40min. The layers were separated; the organic extracts was washed with brine (50ml), dried and concentrated in vacuo to a slurry solid, which was purified by flash chromatography (eluting with CH-EA 10; 1) to give the title compound as an orange solid (7.0g). T.I.c. CH-EA (10:1), Rf0.68. M.p. 106-109°

Intermediate 25

2- ( 1 -Adamantvlmethvlamino)-aniline

A solution of potassium carbonate (23.2g) and sodium hydrosulfite (20.9g) in water (150ml) was added to a mixture of intermediate 24 (6.9g) in ethanol

L 5 9 0 0 / V 5 ,'d/dV

1160CH

AP. Ο Ο 4 9 2 acidified with cone, hydrochloric acid until pH=3. The mixture was then basified with a 10% sodium hydroxide solution until pH=10 and concentrated to half volume. The residue was extracted with ethyl acetate (2x300ml); the combined extracts were washed with brine (150ml),dried and concentrated in vacuo to a residue, which was purified by flash chromatography (eluting with CH-EA 10:2) to give the title compound as a grey solid (5.0g). T.I.c. CH-EA (10:2), Rf 0.36. M.p. 101-104°.

Intermediate 26

N-1-Adamantylmethvl-N'-i2-(4-morpholino)ethvl1-1.2-phenylenediamine

A solution of intermediate 25 (1.3g), sodium iodide (0.76g) and 2-(4morpholino)ethyl chloride hydrochloride (0.94g) in dry dimethylformamide (40ml) was heated at 160° for 4h under a nitrogen atmosphere. The solution was cooled to 23° and concentrated in vacuo. The residue was diluted with ethyl acetate (150ml) and washed with a 10% sodium hydroxide solution (50ml) and brine (50ml). The organic layer was dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with CH-EA 6:4) to give the title compound as a brown oil (0.55g). T.I.c. CH-EA (1:1), Rf=0.39.

Intermediate 27

1-(1 -Adamantylmethyl)-2,4-dioxo-5-f2-(4-morpholino)ethvn-3-phenylhydrazono2,3.4.5-tetrahvdro-1 H-1.5-benzodiazepine

A solution of the intermediate 26 (0.5g) in ethyl acetate (20ml) was added, dropwise, to a solution of the 2-phenylhydrazonomalonyldichloride (0.4g) in ethyl acetate (30ml) at 23° under a nitrogen atmosphere. After complete addition the solution was heated at 80° for 1h. The solution was cooled to 23° and washed with a 10% sodium hydroxide solution (30ml) and brine (50ml). The organic layer was dried, concentrated in vacuo and the residue was purified by flash chromatography (eluting with CH-EA 1:5) to give the title compound as a yellow solid (0.45g). M.p. 110-120°. T.I.c. CH-EA (1:5), Rf 0.48.

AP/P/ 9 4 / 0 0 6 57

Intermediate 28

-(1 -Adamantylmethyl)-3-amino-2.4-dioxo-5-f2-(4-morpholino)ethvn-2,3.4.5-

1160CH

Zinc dust (0.4g) was added to a solution of the intermediate 27 (0.45g) in glacial acetic acid (20ml). The mixture was stirred at 23° for 1,5h, then decanted from zinc. The organic layer was concentrated in vacuo, then diluted with ethyl acetate (50ml) and washed with a 10% sodium hydroxide solution (20ml) and brine (20ml). The organic layer was dried and concentrated in vacuo to a yellow oil, which was purified by flash chromatography (eluting with EA-MeOH 10:1) to give the title compound as a white solid f0.25gl M.p.75-80’. T.I.c. EA-MeOH 10:1, Rf0.11.

Intermediate 29

N-( 1 -Adamantvlmethyl)-N'-[2-( 1 -pyrrolidino)ethvl1-1,2-phenvlenediamine

A solution of intermediate 25 (2.0g), sodium iodide (1.17g) and 2-(1pyrrolidinojethyl chloride hydrochloride (1.33g) in dry dimethylformamide (40ml) was heated at 160° for 4h under a nitrogen atmosphere. The solution was cooled to 23° and concentrated in vacuo. The residue was diluted with ethyl acetate (150ml) and washed with a 10% sodium hydroxide solution (50ml) and brine (50ml). The organic layer was dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with EA-MeOH 95:5) to give the title compound as a brown oil (0.54g). T.I.c. EA-MeOH (9:1), Rf=0.33.

Intermediate 30

-(1 -Adamantvlmethvl)-2.4-dioxo-5-f2-( 1 -pvrrolidino)ethvl1-3-phenvlhvdrazono2.3,4,5-tetrahvdro-1 H-1.5-benzodiazepine

A solution of the intermediate 29 (0.5g) in ethyl acetate (20ml) was added, dropwise, to a solution of the 2-phenylhydrazonomalonyldichloride (0.416g) in ethyl acetate (30ml) at 23θ under a nitrogen atmosphere. After complete addition the solution was heated at 80° for 3h. The solution was cooled to 23° and washed with a 9M ammonium hydroxide solution (25ml) and brine (50ml). The organic layer was dried, concentrated in vacuo and the residue was purified by flash chromatography (eluting with EA-MeOH 10:1) to give the title compound as a yellow solid (0.27g). M.p. 105-110°. T.I.c. EA-MeOH (10:1), Rf 0.44.

AP/P/ 94/00657

1160CH

AP.00492

1-(1-Adamantylmethvl)-3-amino-2,4-dioxo-5-f2-(1-pyrrolidino)ethvH-2,3,4,5tetrahydro-1 H-1.5-benzodiazepine

Zinc dust (0.23g) was added to a solution of the intermediate 30 (0.25g) in glacial acetic acid (20ml). The mixture was stirred at 23° for 15min. The mixture was decanted from zinc, diluted with further ethyl acetate (25ml) and washed with a 10% sodium hydroxide solution (50ml) and brine (50ml). The organic layer was dried and concentrated in vacuo to a yellow oil, which was purified by flash chromatography (eluting in gradient from EA-MeOH 9:1 to EA-MeOH 7:3) to give the title compound as a white foam (0.15g). T.I.c. EA-MeOH 7:3, Rf 0.3.

Intermediate 32

N-d-AdamantylmethvD-N'-fS'-d.l dimethvlethvloxvcarbonvl)-2^,2^,-dimethvl-4^,methvlen-1,2-phenvlene diamine

To a solution of intermediate 25 (0.1 OOg) and 1,1 -dimethylethyl (R) -4-formyl15 2,2-dimethyl-3-oxazolidinecarboxylate (0.125g) in methanol (10ml), acetic acid (0.027 ml) and sodium cyanoborohydride (0.050g ) were added The solution was stirred at 23’ for 1 h, then saturated sodium hydrogen carbonate solution was added ( 50 ml) and the reaction mixture extracted with ethyl acetate (50ml). The organic extracts were washed with brine (50ml), dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with CH-EA 9/1) to give the title compound as a foam (0.100g). T.I.c. CH-EA (5:25), Rf=0.4.

Intermediate 33

1-d-Adamantylmethvh-2.4-dioxo-5-i3'-d.1-dimethyl ethvloxv carbonvl)-2'·2'dimethvM'-methvlen-oxazolidinel -3-phenylhvdrazono-2.3,4,5-tetrahydro-1H1.5-benzodiazepine

A solution of the intermediate 32 (5.8g) in THF (20ml) and a solution of phenylhydrazonomalonyldichloride (4.5g) in THF ( 50 ml) were added , dropwise, to a suspension of Potassium carbonate in THF (20 ml) at 23’ under a nitrogen atmosphere. After complete addition the solution was heated at 80’ for 2h. The solution was cooled to 23’ and washed with a 10% sodium hydroxide solution (30ml) and brine (50ml). The organic layer was dried, concentrated in vacuo and the residue was purified by flash chromatography

AP/P! 94/00657

BAD ORIGINAL &

1160CH (eluting with CH-EA 8/2) to give the title compound as a foam (4.9g). . T.I.c. CH-EA (2:1), Rf0.8.

Intermediate 34

1 -(1-Adamantvlmeth vl)-3-ami no-2,4-dioxo-5-f 3'-/1.1-di methyl ethvloxy carbonvl)2^,,2'-dimethvl-4^,-methvlen-oxazolidine1 -2,3.4,5-tetrahvdro-1 H-1.5benzodiazepine

10%Pd /C (0.33g ) and p-toluensulfonic acid ( 0.325 g) were added to the solution of the intermediate 33 (1 .Og) in methanol (100ml). The mixture was hydrogenated at 23°at 4 atm. for 1 h, then filtered on celite, and concentrated in vacuo to give the title compound as a white solid (0.25g). T.I.c. EA-MeOH 26:4, Rf 0.4.

Intermediate 35

N-f1 -(1-Adamantanemethyl)-2.4-dioxo-5-f3-hvdroxv-2(R)(dimethvlethvloxvcarbonvl)amino-1-propvn-2.3.4.5-tetrahvdro-1H-1.5benzodiazepin-S-vll-N'-phenylurea ISOMER 1 and ISOMER 2

The solution of the intermediate 34( 3.30 g) in trifluoro acetic acid/dichloromethane (50 ml; 0.5 M ) was stirred at 20° for 1 h; 5% sodium hydrogen carbonate solution (100 ml) was added , the organic phase was separated , washed with brine (100 ml), dried and concentrated under vacuum to give a residue wich was taken up in acetonitrile ( 50 ml).

To the resulting solution, phenyl isocyanate (0.072ml) was added; the reaction mixture was stirred at 23° for 15h, concentrated under vacuum to give the title compound as a mixture of isomers 1 and 2. This mixture was separated by purified by flash chromatography on silica using CH/EA 9/1 as eluant to give the title compound (ISOMER 1 ) as a white solid (0.6g). M.p.188^o. T.I.c. CH-EA 1:1, Rf0.6. Rf0.6. IR: 3431 (NH, OH), 1699 (C=O) cm-1; 1H-NMR: 7.66 (m); 7.46-7.24 (m); 7.05 (m); 6.99 (s); 6.34 (d); 5.42 (d);5.16 (d); 4.39 (d); 4.30-4.18 (m); 3.96-3.80 (m); 3.66 (t); 3.49 (dd); 3.19 (d); 1.83 (m); 1.66-1.10 (m); 1.44 (s).

Continuing the elution, some mixed fractions were obtained (0.34 g) and then the title compound (ISOMER 2 ) was eluted ( 0.9g ) T.I.c. CH-EA 1:1, Rf 0.6. IR: 3364 (NH, OH), 1697 (C=O) cm-1; 1H-NMR: 7.70 -7.10 (m); 6.92 (m);6.69( d ); 5.37 (bd;); 5.16 (d); 4.38 (d); 4.24-4.0 (m); 3.96-3.68 (m); 3.70-3.44 (m); 3.35

AP/P/ 94/00657

1160CH

Intermediate 36

N-Cvclohexvlmethyl-1,2-phenylenediamine

A solution of 1,2-phenylenediamine (5.0g), cyclohexylomethyl bromide (7.0g) and sodium iodide (7.0g) in dry dimethylformamide (250ml) was stirred at 32° for 24h under a nitrogen atmosphere. The solution was diluted with water (200ml) and extracted with ethyl acetate (4x200ml); the combined organic extracts were washed with brine (500ml), dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with CH-EA 8:2) to give the title compound as a white solid (1.8g). T.I.c. HC-EA (1:1), Rf=0.55. IR:

3400, 3371 and 3271 (NH2 and NH) cm-¹

Intermediate 37

N-Cvclohexylmethyl-N'-f2-(diethvlamino)ethvM-1,2-phenvlenediamine a solution of Intermediate 36 (2.42g), sodium iodide (1.22g) and 2diethylaminoethyl chloride hydrochloride (2.0g) in dry dimethylformamide (100ml) was heated at 160° for 4h under a nitrogen atmosphere. The solution was cooled to 23° and concentrated in vacuo. The residue was diluted with a 10% sodium hydroxide solution (100ml) and extracted with ethyl acetate (200ml). The organic layer was washed with brine (150ml), dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with CH-EA 4:6) to give the title compound as a brown oil (1.7g). T.I.c.

( CHEA (1:1), Rf=0.26. IR: 1601 (C=C) cm-¹.

Intermediate 38

1-Cvclohexvlmethvl-2.4-dioxo-5-f2-(diethvlamino)ethvl1-3-phenylhvdrazono2.3.4,5-tetrahvdro-1 H-1.5-benzodiazepine

A solution of the Intermediate 37 (1,7g) in ethyl acetate (50ml) was added, dropwise, to a solution of the 2-phenylhydrazonomalonyldichloride (1.37g) in ethyl acetate (50ml) at 23° under a nitrogen atmosphere. After complete addition the solution was heated at 80° for 4h. The solution was cooled to 23° and washed with a 10% sodium hydroxide solution (50ml) and brine (50ml). The organic layer was dried, concentrated in vacuo and the residue was purified by flash chromatography (eluting with CH-EA 6:4) to give the title compound as a

AP/P/ 94/00657

1160CH yellow foam (1.0g). T.I.c. CH-EA (1:1), Rf0.34. IR: 3441-3186 (NH); 1661 (C=0) cm·¹.

Intermediate 39

3-Amino-1-cvclohexvlmethyl-5-i2-(dimethylamino)ethyl1-2,4-dioxo-2.3.4.5tetrahvdro-1 H-1,5-benzodiazepine

Zinc dust (0.3g) was added to a solution of the intermediate 38 (0.38g) n glacial acetic acid (5ml). The mixture was stirred at 23° for 3h, then decanted from zinc. The filtrate was basified with a 10% sodium hydroxide solution until pH=10 and extracted with ethyl acetate (2x40ml). The combined organic extracts were washed with brine (60ml), dried and concentrated in vacuo to an oil, which was purified by flash chromatography (eluting with DCM-MeOH 9:1) to give the title compound as a yellow solid (0.17a). M.p.94-5°. T.I.c. DCMMeOH 85:15, Rf 0.78. IR:1695 and 1664 (C=O) cm*¹

Intermediate 40

5-Fluoro-N-f2-(4-morpholino)ethvn-2-nitroaniline

A solution of 2-(N-morpholino)ethylamine (2.45g) in tetrahydrofuran (10ml) was added dropwise to a solution of 2,4-difluoronitrobenzene (3.0g) in tetrahydrofuran (30ml) and the mixture was stirred at 23° for 1.5h. The mixture was concentrated in vacuo, crystallized from DCM-Petrol and purified by flash chromatography (eluting with CH-EA 1:1) to give the title compound as a yellow solid (3.0g). T.I.c. CH-EA (1:1) Rf= 0.39. M.p. 103-4°. IR (nujol): 3400 (N-H); 1632 (C=C); 1570, 1312 (NO2) cm'¹.

Intermediate 41

5-Fluoro-N'-f2-(4-morpholino)ethvn-1.2-benzenediamine

Potassium carbonate (9.7g) and sodium hydrosulfite (8.4g) were added to a suspension of intermediate 40 in ethanol-water 1:1 (150ml) and the mixture was stirred at 23° for 1h. The mixture was concentrated in vacuo, acidified to pH= 3 with cone, hydrochloric acid and extracted with ethyl acetate (150ml). The aqueous layer was basified with a 10% sodium hydroxide solution then extracted with ethyl acetate(2x150ml). The organic layer was washed with brine (200ml), dried and concentrated in vacuo to give the title compound as a brown

AP/P/ 94/00657

1160CH

AP.00492 oil (1.9g). T.I.c. EA-MeOH (9:1) Rf= 0.28. IR (nujol) 3337 (NH); 1614 (C=C) cnv 1

Intermediate 42a

N-( 1 -Adamantvlcarbonvl)-4-fluoro-N^,-F2-(4-morpholino)ethvH-1.25 benzenediamine

A solution of 1-adamantanecarbonyl chloride (1.78g) in dry THF (30ml) was added dropwise to a mixture of intermediate 41 (1.9g) and triethylamine (1.36ml) in dry THF (70ml). The mixture was heated at 60° for 1.5h, then the solvents were evaporated in vacuo. The residue was taken up with ethyl acetate f 10 (200ml), washed with water (100ml) and brine (50ml), dried and concentrated in vacuo to give the title compound as a white solid (3.23g). T.I.c. CH-EA (1:1) Rf =0.31. M.p. 172-40. IR (nujol) 3375, 3314 (NH); 1647 (C=O); 1618, 1600 (C=C) cm^-1.

Intermediate 42b

N-( 1 -Adamantvlmethvl)4-fluoro-N^,-f2-(4-morpholino)ethvn-1,2-benzenediamine

A solution of Vitride [sodium dihydro-bis (2-methoxyethoxy)aluminate] (5.7ml) in toluene (10ml) was added dropwise over 15min to a cooled (0°C) suspension of intermediate 42a (3.23g) in toluene (40ml). The mixture was stirred at 0° for further 10min, then at 23° for 30min. The reaction was quenched by adding ( ethyl acetate (20ml) at 0°, over 15min. After additional 15min, the mixture was diluted with more ethyl acetate (100ml) and washed with water (3x100ml); the ( aqueous layer was extracted with ethyl acetate (200ml), the combined organic extracts were washed with brine (150ml), dried and concentrated in vacuo . The residue was purified by flash chromatography (eluting with CH-EA 7:3) to give the title compound as a wax (2.2g). T.I.c. CH-EA (1:1) Rf 0.49. IR (nujol) 3300 (NH); 1612(C=C)cm-i

Intermediate 43

1-Adamantylmethyl-2.4-dioxo-7-fluoro-5-f2-(4-morpholino)ethvH-3phenvlhvdrazono-2.3,4.5- tetrahvdro-1 H-1.5-benzodiazepine A solution of phenylhydrazonomalonyl dichloride (1.43g) in ethyl acetate (50ml) was added dropwise to a solution of intermediate 42b (2.05g) in ethyl acetate (30ml) and the mixture was heated at 60° for 3h. The mixture was diluted with ethyl acetate (150ml) and washed with a 5% sodium hydroxide solution (100ml);

AP/r/ 94/00657 bad original

1160CH the aqueous layer was reextracted with ethyl acetate (100ml) and the combined organic layers were washed with brine (100ml), dried and concentrated in vacuo. The residue was purified by flash chromatography (eluting with CH-EA 7:3) to give the title compound as a yellow foam (2.05g). T.I.c. CH-EA (1:1) Rf= 0.42. IR (nujol) 1663 (C=O); 1603, 1590 (C=C) cm’¹.

Intermediate 44

1-Adamantvlmethvl-3-amino-2.4-dioxo-7-fluoro-5-f2-(4-morpholino)ethvn2.3.4,5- tetrahvdro-1 H-1.5-benzodiazepine

Zinc dust (1.76g) was added to a solution of intermediate 43 (2.05g) in glacial acetic acid (30ml) and the mixture was stirred at 23° for 4h; then, it was filtered over celite, washing the solid with ethyl acetate, and the filtrate was basified with a 10% sodium hydroxide solution. The layers were separated and the aqueous phase was extracted with ethyl acetate (2x50ml); the combined organic extracts were washed with brine (50ml), dried and concentrated in vacuo. The residue was purified by flash chromatography (eluting with EA-MeOH 9:1) to give the title compound as a white foam (1,1g). T.I.c. EA-MeOH (8:2) Rf 0.5. IR (nujol): 3400 (NH), 1693-1663 (CO); 1605 (C=C) cm-¹.

Intermediate 45

N-(3-methvl-1-butvh-N'-[(2-(4-morpholino)ethyl1-1,2-benzenediamine Glacial acetic acid (1.5ml) was added to a solution of 2-[-2-(4-morpholinoethyl) aminojaniline (5.7g) and 3-methylbutyraldehyde (2.7ml) in methanol (100ml). The mixture was stirred at 23° for 10min., then, sodium cyanoborohydride (3.5g) was added portionwise. Stirring was continued for 3h, then the mixture was concentrated in vacuo, the residue was diluted with ethyl acetate (500ml), washed with a 5% sodium bicarbonate solution (2x100ml) and brine (150ml), dried and the solvents were evaporated in vacuo. Purification of the crude material by flash chromatography (eluting with CH-EA 55:45) afforded the title compound as a colorless oil (3.3g). T.I.c. CH-EA (1:1), Rf 0.33. IR: 1601 (C=O) cm-¹; ¹H-NMR: 6.79; 6.66 (m); 3.71; 3.13 (m); 2.69; 2.49; 1.79; 1.58; 0.97.

Intermediate 46

2.4-Dioxo-1-(3-methvl-1-butvl)-54(2-(4-morpholino)ethyH-3-phenvlhvdrazonoAP/P/ 9 4/ 0 0 6 57

1160CH

AP. Ο Ο 4 9 2

Γ ¹⁰ (

A solution of 2-phenylhydrazonomalonyl dichloride (3.6g) in ethyl acetate (250ml) was added dropwise to a solution of intermediate 45 (3.3g) in ethyl acetate (150ml) and the mixture was stirred under reflux for 2h. The mixture was concentrated in vacuo and the residue was purified by flash chromatography (eluting with EA), to give the title compound as a yellow solid (3.6g). T.I.c. CHEA (1:1), Rf 0.13. M.p. 76-8°. IR. 1653 and 1626 (C=O) cm-¹;

Intermediate 47

3-Amino-2.4-dioxo-1-(3-methvl-1-butvl)-5-f2-(-4-morpholino)ethvn-2.3.4.5tetrahvdro-1 H-1.5-benzodiazepine

Zinc metal (3.8g) was added portionwise to a solution of intermediate 46 (3.6g) in glacial acetic acid (60ml); the mixture was stirred at 23° for 15min., then it was diluted with ethyl acetate (150ml) and filtered, washing the solid with ethyl acetate (100ml) and a 10% sodium hydroxide solution (20ml). More 10% sodium hydroxide solution (150ml) was added to the filtrate, until pH=10, then the solution was extracted with ethyl acetate (150ml). The organic layer was washed with brine (100ml), dried and concentrated in vacuo. The residue was purified by flash chromatography (eluting with EA-MeOH 10:1) to give the title compound as a light yellow solid (1.5g). T.I.c. EA-MeOH (10:1), Rf 0.37. M.p. 117-9°C. IR: 1691 (C=0)cm'1;

AP/P/ 94/00657

Intermediate 48

N-f2-(Hexamethvleneimino)ethvn-N'-phenvl-1.2-benzenediamine

N-Phenyl-1,2-benzenediamine (2.0g) and 2-(hexamethyleneimino)-ethylchloride hydrochloride (2.57g) were added to a mixture of potassium carbonate (4.48g) and potassium iodide (2.16g) in dry xylene and the mixture was refluxed under a nitrogen atmpsphere for 2h. The mixture was diluted with methylene chloride (100ml), washed with a 5% ammonia solution (50ml), water (50ml) and brine (70ml), dried and the solvents were evaporated in vacuo. Purification of the crude material by flash chromatography (eluting with CH-EA 6:4) to give the title compound (2.09g) as a dark oil. T.I.c. CH-EA (4:6), Rf 0.45. IR: 3252 (NH); 1597 (C=C)cm-1;

Intermediate 49

BAD ORIGINAL gj

1160CH

2.4-Dioxo-1-f2-(hexamethvleneimino)ethyl1-5-phenyl-3-phenylhvdrazono2.3,4,5-tetrahvdro-1 H-1,5-benzodiazepine

A solution of 2-phenylhydrazonomalonyl dichloride (1.81g) in ethyl acetate (115ml) was added dropwise to a solution of intermediate 48 (2.08g) in ethyl acetate (115ml) and the mixture was stirred at 23° for 1 h, then at 50° for 1h. The mixture was washed with a 10% sodium hydroxide solution (100ml) and brine (100ml), dried and concentrated in vacuo. The residue was purified by flash chromatography (eluting with EA), to give the title compound as a yellow foam (2.03g). T.I.c. EA, Rf 0.34. IR: 1664 (C=O); 1591 (C=C) cm-1;

r 10

Intermediate 50

3-Amino-2.4-dioxo-1-i2-(hexamethvleneimino)ethvn-5-phenyl-2.3.4.5-tetrahydro1 H-1,5-benzodiazepine

Ammonium formate (0.656g) and 10% Palladium on charcoal (0.463g) were 15 added to a solution of intermediate 49 (0.50g) in dry methanol (20ml). The mixture was refluxed for 30min under a nitrogen atmosphere, then cooled to

23°C and filtered over celite. The filtrate was concentrated in vacuo; the residue was taken up in diethyl ether (50ml) and extracted with a 10% hydrochloric acid solution (50ml). The aqueous layer was neutralized with solid sodium bicarbonate, then extracted with ethyl acetate (2x50ml). The organic layer was washed with brine (50ml), dried and concentrated in vacuo to give the title compound as an orange foam (0.36g). T.I.c DCM-MeOH 95:5, Rf 0.42.

(

Intermediate 51 (S)-(+)-2-(4-toluenesulohonyloxy)-phenvlacetic acid methvl ester

Method A) (S)-(+)-methyl mandelate (3.0 g) and triethylamine (6.13 ml) were dissolved in dry dichloromethane (40 ml). The mixture was cooled to 0 ⁰ then 4toluenesulphonyl chloride (6.87 g) was added under stirring. The solution was kept at this temperature for 40min. and then was allowed to warm to 23 ° during

20min. After this time, the mixture was diluted with dichloromethane (20 ml), washed with brine (50 ml), dried and concentrated in vacuo. The crude material was purified by flash chromatography (eluting with CH/EA 5:1 then 2:1) to give the title compound as a white wax (5.75 g). T.I.c. (CH/EA 2:1) Rf=0.54, HPLC: (+)/(-)=91.4/8.6 e.e.=82.8%, M.p =57-58

AP/P/ 94/00657

1160CH

AP . Ο Ο 4 9 2

Method Β) (S)-(+)-methyl mandelate (3.0 g) and pyridine (2.9 ml) were dissolved in dry dichloromethane (40 ml). The mixture was cooled to 0° then 4toluenesulphonyl chloride (6.87 g) was added under stirring. The solution was kept at this temperature for 15min. and then was allowed to warm to 23° . After

4h the mixture was diluted with dichloromethane (50 ml), washed with HCI 5% (80 ml) and brine (80 ml), dried and concentrated in vacuo. The crude material was purified by flash chromatography (eluting with CH/EA 4:1 then 2:1) to give a colorless oil which was further purified by flash chromatography (eluting with CH/EA 5:1) to give the title compound as a white wax (2.2 g). T.I.c. (CH/EA 2:1)

Rf=0.54, HPLC: (+)/(-)=99.8/0.2 e.e.=99.6%, M.p =57-58 ⁰

Intermediate 52 f1-(1-Adamantvlmethvl)-2.4-dioxo-5-f2-(4-morpholino)ethvn-2.3.4.5-tetrahvdro1H-1.5-benzodiazepin-3-vll-amino phenylacetic acid methyl ester (isomer 1 and isomer 2)

Diisopropylethylamine (0.348ml) was added to a mixture of intermediate 28 (0.905g) and intermediate 51 (1.28g) in dry tetrahydrofuran (30ml). The mixture was refluxed for 8h, then it was diluted with dichloromethane (100ml), washed with a saturated ammonium chloride solution (100ml) and brine (100ml), then dried and concentrated in vacuo. The crude material was purified twice by flash chromatography (eluting with CH-EA in gradient from 1:3 to 1:9, then with EAMeOH 4:1), to give :

title compound(isomer 1)(0.336 g) as white foam as white foam. T.I.c. (EAMeOH 24:1) Rf=0.65, IR: 1742, 1700, 1666 (C=O).

title compound(isomer2)(0.081 g). as a white foam.T.I.c. (EA-MeOH 24:1) Rf=0.61, HPLC: d.e =90.6%,

Intermediate 53

N-(tert-butoxycarbonvl)-D-phenvlalanine

Di-tert-butyldicarbonate (4,32g) was added to a solution of D-phenylalanine (3g) in a mixture of dioxan/water (2:1, 54ml) and sodium hydroxide 1N (18ml). The mixture was stirred at 23° for 3h, dioxan was evaporated in vacuo and the chilled water phase was extracted with ethyl acetate (30ml). The water solution was acidified at pH=3 by the addition of solid citric acid and extracted with ethyl

AP/P/ 9 4 / 0 0 6 57

1160CH acetate (2x30ml). The organic layer was washed with brine (30ml), dried and evaporated to give the crude title compound as an oil (4,3g).

1H-NMR (CDCI3):7.4-7.1(m),5.0-4.5(bm), 3.3-2.7(bm), 1.4(s)

Intermediate 54

N-(1-Adamantvlmethvl-5-f2-(4-morpholino)ethyll-2.4-dioxo-2.3.4.5.tetrahydro1.5-benzodiazepin-3vl)-2-D-{3-tertbutoxvcartoonvl)-3-phenvlpropionamide

N. N' dicyclohexylcarbodiimide (0,784g) and 1-hydroxybenzotriazole (0,565g) were added to a solution of intermediate 53 in ethyl acetate (150ml). The solution was stirred at 23° for 2h, then a solution of Intermediate 28 (1,5g) in ethyl acetate (10ml) was added. The resulting solution was stirred for 3h at 23°, then filtered and concentrated in vacuo. The residue was purified by flash chromatography using a gradient of EA-CH (1:1 to pure EA) as eluant to afford the title compound (1,84g).as a foam T.I.c. EA-CH (2:1) Rf= 0.2. IR: 3400 (N-H) cm-1707,1672 (C=0)cm-1.

Intermediate 55

N41-Adamantylmethvl-5-r2-(4-morpholino)ethvn-2.4-dioxo-2.3.4,5,tetrahvdro1H-1.5-benzodiazepine-3-yl)-2-D-amino-3-phenvlpropionamide (isomer 1 and isomer 2)

Intermediate 54 (1,84g) was dissolved in a mixture of trifluoroacetic acid (6ml) and dichloromethane (6ml) and stirred at 23° for 30min. The reaction mixture was concentrated in vacuo and triturated with diethyl ether to give the trifluoroacetic salt of the title compound, which was filtered and dried (1.78g). This salt was suspended in ethyl acetate (50ml) and extracted with a 5% ammonia solution (70ml). The organic layer was washed with brine, dried and concentrated in vacuolo give a white foam (1.24g). Separation of the two diastereomers was achieved by flash chromatography eluting with a gradient of EA-MeOH (98:2 to 95:5) to give :

title comooundfisomer 1) (0.618a)as a white foam T.I.c. EA-MeOH (9.25 : 0.75) Rf =0.38 title compound (isomer 2 )(0.440g) as a white foam. T.I.c. EA-MeOH (9.25 :

O. 75) Rf = 0.22. I.R :1705,1666 (C=O) cm-1.

AP/P/ 9 4 / 0 0 6 57

1160CH

AP.00492

N41-Adamantvlmethvl-5-f2-(4-morpholino)ethyri-2,4-dioxo-2,3.4.5- tetrahvdro1H-1,5-benzodiazepin-3-vl)-3-phenvl-2-D-(3-phenvlthioureido)-propionamide

Phenylisothiocyanate (0,149g) was added to a solution of intermediate 55 (isomer 1) (0,61 g) in dichloromethane (50ml). The solution was stirred at 23° for 3h and at 50° for 30min. The solvent was evaporated and the residue was purified by flash chromatography using EA-CH (1:1) as eluant to afford the title compound as a foam (0,66g) T.I.c. EA-CH (1:1) Rf= 0.38. IR 1705,1666 (C=O) cm-1.

Intermediate 57 (-)3-Amino-1-(1-adamantvlmethvl)-2.4-dioxo-5-f2-(4-morpholino)ethvn-2,3,4,5tetrahydro-1 H-1.5-benzodiazepine

Method A:

20% Palladium (II) hydroxide on charcoal (0.218g) was added to a solution of intermediate 52 (isomer 1) (0.187g) in methanol (10ml). The mixture was hydrogenated at atmospheric pressure for 5h, then filtered on a celite pad. After evaporation of the solvents, the crude material was purified by flash chromatography (eluting with CH-EA 1:1 then with EA-MeOH 3:2) to give the title compound as a white solid (0.140g). T.I.c. (EA-MeOH 1:1) Rf=0.44. M.p.

180-5°C. aD= -36°. -IR 3480-3350 (N-H), 1695, 1664 (C=O) cm-1.

Method B

Intermediate 57(0.65g) was dissolved in trifluoroacetic acid (15ml) and stirred at 60° for 30min. The solution was concentrated in vacuo, the residue was diluted with ethyl acetate (60ml) and washed with a 5% sodium bicarbonate solution (20ml) and brine. The organic phase was dried and concentrated in vacua, the residue was purified by flash chomatography using EA-CH (1:1) then EAMeOH (9:1) as eluants to afford the title compound (0,05g) and recovered starting material (0,508g).

product, obtained by reaction of title compound with phenylisocyanate, had the same retention time (5.2min) as isomer I of Example 11.(column: Pirkle DDNBPG C5,(25x2.4) eluent DCM-IPA 93:7.)

The recovered starting material (0,169g) was reprocessed by stirring in trifluoroacetic acid (10ml) at 40° for 22h. After usual work-up, further title

AP/P/ 9 4/ 0 0 6 57

1160CH

EXAMPLE 1

N-f2.4-Dioxo-1-(3-methyl-1-butyl)-5-r2-(4-morpholinvl)ethvn-2.3,4,5-tetrahvdro1H-1 .S-benzodiazepin-S-vll-N'-phenylurea

Method A:

Sodium hydride (19.8mg) was added to a solution of the intermediate 7 (110mg) in dry DMF (5ml) under a nitrogen atmosphere. The mixture was stirred at 230 for 30min, then 4-(2-chloroethyl)morpholine hydrochloride (67.6mg) was added. The mixture was heated at 700 for 5h. The mixture was cooled to 23°, then diluted with a 5% sodium hydrogen carbonate solution (30ml) and extracted with ethyl acetate (3x30ml). The combined organic extracts were washed with brine (60ml), dried and concentrated in vacuo to an oil. The latter was purified by flash chromatography (eluting with EA) and the solid obtained was further purified by trituration with diethyl ether to give the title compound as a white solid (78mg). M.p. 129-1300. T.I.c. EA-MeOH (95:5), Rf 0.46.

Method B:

A mixture of the intermediate 7 (50mg), potassium carbonate (54mg), 4-(2chloroethyl)morpholine hydrochloride (26.6mg), acetone (10ml) and water (1ml) was stirred at 750 for 17h. The suspension was cooled to 230; inorganic compounds were filtered off and the filtrate was concentrated in vacuo. The residue was triturated with acetonitrile to give the title compound as a white solid (45mg). M.p. 129-1300. T.I.c. EA-MeOH (95:5), Rf 0.46. IR :3400 (NH), 1695 and 1637 (C=O), 1601 and 1558 (C=C) cm-1; ¹H-NMR :7.6-7.54 (m);

ί 7.46-7.24 (m); 7.05 (t); 6.75 (s); 6.22 (d); 5.09 (d); 4.4-4.2 (m); 3.8-3.6 (m); 2.62.35 (m); 1.6-1.35 (m); 0.88 (d); 0.86 (d).

EXAMPLE 2

N-f2.4-Dioxo-1-(3-methvl-1-butyl)-5-[2-(1-piperidino)ethvll-2.3,4,5-tetrahydro1 H-1,5-benzodiazepin-3-vn-N'-phenylurea

A mixture of the intermediate 7 (50mg), potassium carbonate (54mg), 4-(230 chloroethyl)piperidine hydrochloride (26.33mg), acetone (10ml) and water (1ml) was stirred at 75° for 18h. The suspension was cooled to 23©; inorganic compounds were filtered off and the filtrate was concentrated in vacuo. The residue was triturated with diethyl ether to give the title compound as a white solid (44mg). M.p. 107-90. T.I.c. EA-MeOH (95:5), Rf 0.2. IR :3429 and 3192

AP/P/94/ 0 0 6 57

1160CH

AP.0 0 4 9 2 (m); 7.05 (t); 6.75 (s); 6.22 (d); 5.08 (d); 4.4-4.2 (m); 3.8-3.65 (m); 2.5-2.3 (m); 1.56-1.3 (m); 0.87 (d); 0.84 (d).

EXAMPLE 3

N-f5-f2-(Dimethvlamino)ethvl1-2.4-dioxo-1-(3-methyl-1-butvl)-2.3.4.5-tetrahvdro1 H-1 .S-benzodiazepin-S-vn-N’-phenvIurea

A mixture of the intermediate 7 (50mg), potassium carbonate (54mg), 2dimethylaminoethylchloride hydrochloride (20.6mg), acetone (10ml) and water (1ml) was heated at 75° for 20h. The suspension was cooled to 23°; inorganic compounds were filtered off and the filtrate was concentrated in vacuo. The residue was purified by flash chromatography (eluting with EA-MeOH 95:5) to give the title compound as a white solid (45mg). M.p. 159-1610. T.I.c. EAMeOH (95:5), Rf 0.39. IR : 3350 (NH), 1695 and 1641 (C=O), 1601 (C=C) cm1; 1H-NMR :7.54-7.2 (m); 7.055 (t); 6.71 (s); 6.20 (d); 5.08 (d); 4.4-4.25 (m); 3.8-3.6 (m); 2.5-2.3 (m); 2.17 (s); 1.5 (m); 1.45-1.35 (m); 0.87 (d); 0.85 (d).

EXAMPLE 4

N-f2.4-Dioxo-1-(3-methvl-1-butvl)-5-l2-(4-morpholino)ethvn-2.3.4.5-tetrahydro1 H-1.5-benzodiazepin-3-vn-N’-(4-methoxvphenvl)urea

A mixture of the intermediate 8 (0.175g), potassium carbonate (0.178g), 4-(2chloroethyl)morpholine hydrochloride (0.087g), acetone (20ml) and water (2ml) was stirred at 75° for 18h. The suspension was cooled to 230; inorganic compounds were filtered off and the filtrate was concentrated in vacuo. The residue was purified by flash chromatography (eluting with EA-MeOH 98:2) to give the title compound as a white solid (178mg). M.p. 161-3°. T.I.c. EA-MeOH (9:1), Rf 0.53. IR : 3346 (NH), 1728, 1700 and 1653 (C=O) cm-1; 1H-NMR :7.54 (m); 7.42-7.32 (m); 7.26 (d); 6.86 (d); 6.43 (s); 6.06 (d); 5.06 (d); 4.4-4.1 (m); 3.78 (s); 3.8-3.6 (m); 3.62 (t); 2.6-2.2 (m); 1.6-1.3 (m); 0.87 (d); 0.84 (d).

EXAMPLE 5

N-f2,4-Dioxo-1-(3-methyl-1-butvl)-5-i2-(4-morpholinvl)ethvn-2,3,4,5-tetrahvdro1 H-1.5-benzodiazepin-3-vll-N'-(4-hvdroxyphenvl)urea

Aluminium iodide (0.194g) was added to a solution of Example 4 (0.05g) in dry acetonitrile (20ml) under a nitrogen atmosphere. The solution was heated at 90° for 24h. Further aluminium iodide (0.194g) was added and the mixture

AP/P/ 94/00657

BAD ORIGINAL ft

1160CH heated at 90© for further 24h. The mixture was cooled to 23°, diluted with water (5ml) and a 5% sodium thiosulfate solution (25ml) and extracted with ethyl acetate (2x30ml). The combined organic extracts were washed with brine (50ml), dried and concentrated in vacuo to a residue that was triturated with diethyl ether to give the title compound as a white solid (0.030g). M.p. 104-5© (dec). T.I.c. EA-MeOH (95:5), Rf 0.2. IR: 3450 and 3340 (NH and OH), 1697 and 1663 (C=O) cm-1; 1H-NMR :7.55 (m); 7.46-7.32 (m); 7.07 (m); 6.62 (m); 6.46-6.10 (m); 5.07 (d); 4.34 (m); 4.20 (m); 3.9-3.6 (m); 2.7-2.3 (m); 1.8-1.3 (m); 0.86 (d); 0.84 (d).

EXAMPLE 6

N-f5-f2-(diethvlamino)ethvn-2.4-dioxo-1-(3-methyl-1-butvl)-1-2.3.4.5-tetrahvdro1H-benzodiazepin-3-vH-N’-phenylurea

Phenyl isocyanate (0.022ml) was added to a solution of the intermediate 16 (0.07g) in acetonitrile (3.5ml). The reaction mixture was stirred at 23C for 20min, then the solid was filtered , washed with acetonitrile to give the title compound as a white solid (0.066g). M.p 147°.

T. I.e. EA/MeOH 8:2, Rf 0. 64 . IR: 3315 (NH), 1703 and 1666 (C=O) cm-1; 1H-NMR: 7.57 (m); 7.39 (m); 7.37-7.29 (m); 7.04 (t); 6.84 (bs); 6.26 (d); 5.08 (d); 4.36-4.2 (m); 3.74 (m); 3.62 (m); 2.63 (m); 2.49 (q); 1.48 (m);1.38 (m); 0.94 (t); 0.86 (d); 0.83 (d).

EXAMPLE 7

N-M -(1 -Adamantvlmethvl)-5-[2-(dimethvlamino)ethyH-2.4-dioxo-2.3.4.5tetrahvdro-1 H-1.5-benzodiazepin-3-vl1-N'-phenvlurea

A mixture of the intermediate 22 (0.134g) and 80% oil suspension sodium hydride (0.020g) in dry DMF ( 5ml), was srirred at 20° for 15 min., then 2dimethylaminoethylchloride hydrochloride (0.052g) was added and the resulting mixture was heated at 80© for 4h. The suspension was cooled to 23©; diluted with ethyl acetate ( 50 ml) and saturated sodium hydrogen carbonate solution ( 50 ml); the collected organic phases were washed with brine, dried and concentrated in vacuo. The residue ( 0.16g) was purified by flash chromatography (eluting with EA-MeOH 9:1) to give the title compound as a white solid (0.13g). M.p. 150-21©. T.I.c. EA-MeOH (9:1), Rf 0.31. IR : 3400

AP/P/ 94/00657

1160CH

AP.00492 , 10 (

I (m); 6.50 (bs); 6.08 (d); 5.10 (d); 4.39 (d); 4.11-3.79 (m); 3.23 (d); 2.9-2.66 (m);

2.33 (s); 1.83 (m); 1.66-1.40 (m); 1.24 (m).

EXAMPLE 8

N-f1-(1-Adamantylmethvl)-5-f3-(dimethvlamino)propyll-2.4-dioxo-2.3.4.5tetrahvdro-1 H-1 .S-benzodiazepin-S-vll-N’-phenvIurea

A mixture of the intermediate 22 (0.103g) and 80% oil suspension sodium hydride (0.017g),in dry DMF ( 5ml) was stirred at 20° for 15 min., then 3(dimethyfamino)propylchloride hydrochloride (0.043g) was added and the resulting mixture was heated at 80θ for 4h. The suspension was cooled to 230; diluted with ethyl acetate ( 50 ml) and saturated sodium hydrogen carbonate solution ( 50 ml); the collected organic phases were washed with brine, dried and concentrated in vacuo. The residue ( 0.12g) was purified by flash chromatography (eluting with EA-MeOH 9:1) to give the title compound as a white solid (0.09g). M.p. 138-400. T.I.c.DCM-MeOH (9:1), Rf 0.24. IR : 3315 (NH), 1699 and 1639 (C=O),1610 (C=C) cm-1; 1H-NMR :7.52-7.24 (m); ; 7.04 (t); 6.87 (bs); 6.27 (d); 5.09 (d); 4.39 (d); 4.08-3.80 (m); 3.24 (d); 2.45 (m); 2.28 (s); 2.2-2.0(m); 1.98-1.70 (m); 1.6-1.2 (m).

EXAMPLE 9

N-F1-(1-Adamantylmethyl)-2,4-dioxo-5-f2-(4-morpholino)ethyl1-2,3,4,5tetrahydro-IH-benzodiazepin-S-vH-N'-phenylurea

Phenyl isocyanate (0.041ml) was added to a solution of the intermediate 28 (0.16g) in acetonitrile (7ml). The reaction mixture was stirred at 23θ for 30min, then concentrated in vacuo. The residue was triturated with diethyl ether to give the title compound as a white solid (0.1a). M.p. 188-190°. T.I.c. CH-EA 1:1, Rf 0.18. IR: 3317 (NH), 1699 and 1666 (C=O) cm-1; 1H-NMR: 7.80 (m); 7.5-7.2 (m); 7.05 (t); 6.81 (s); 6.24 (d); 5.12 (d); 4.39 (d); 4.14 (m); 3.9-3.6 (m); 3.23 (d); 2.94-2.76 (m); 1.83 (s); 1.7-1.1 (m).

EXAMPLE 10

N-f1 -(1 -Adamantvlmethvl)-2.4-dioxo-5-f2-( 1 -pyrrolidino)ethvl1-2.3.4.5-tetrahvdro1H-benzodiazepin-3-vn-N'-phenvlurea

Phenyl isocyanate (0.026ml) was added to a solution of the intermediate 31 (0.1 g) in dichloromethane (7ml). The reaction mixture was stirred at 23° for

AP/P/ 94/00657

BAD ORIGINAL ft

1160CH

30min, then concentrated in vacuo. The residue was triturated with diethyl ether to give the title compound as a white solid (0.08g). M.p.155-160°. T.I.c. EAMeOH 10:1, Rf 0.43. IR: 3200 (NH), 1695 and 1664 (C=O) cm-1; 1H-NMR: 7.65 (m); 7.4-7.28 (m); 7.03 (t); 6.91 (s); 6.31 (d); 5.11 (d); 4.38 (d); 4.15 (m); 3.88 (m); 3.22 (d); 2.94 (m); 2.66-2.54 (m); 1.86-1.76 (m); 1.58 (d); 1.46 (d); 1.25 (d); 1.20(d).

EXAMPLE 11

N-f1-(1-Adamantvlmethvl)-2.4-dioxo-5-f2-(4-morpholino)ethvl1-2.3.4.5tetrahydro-IH-benzodiazepin-S-vll-N'-phenylurea Isomer 1 and Isomer 2

METHOD A :

The compound of Example 9 was resolved into pure enantiomers (isomer 1 and isomer 2) by preparative HPLC (Pirkle D-DNBPGC 25x2.4cm and dichloromethane-isopropyl alcohol 93/7 v/v as elutant..

Title compound Isomer 1 retention time tr 5.2min, alpha D = -50.0 (CHCI₃).

Title compound Isomer 2 retention time tr = 7.8min, alpha D = +42.0 (CHCI₃).

METHOD B:

Phenylisocyanate (0.049ml) was added to a solution of intermediate 57 .obtained by method A,(0.102g) in dry acetonitrile (5ml) and the mixture was stirred at 23°for 5 min. The solid was filtered off, washed with acetonitrile and triturated with EE-CH 1:1 to give the title compoundf isomer 1) (0.094g) as a white solid. T.I.c. (EA-CH 1:1) Rf=0.30, HPLC: e.e =94%. «ο= -40°. M.p.: 157159°C.

1H-NMR: 7.80 (m), 7.44-7.24 (m), 7.05 (m), 6.75 (bs), 6.21 (bd), 5.12 (d), 4.39 (d), 4.14 (m), 3.78 (m), 3.75 (m), 3.22 (d), 2.93 (m), 2.75 (m), 2.57 (m), 1.83 (m), 1.64-1.18 (m). IR: 3400 (N-H), 1699, 1668, 1641 (C=O).

EXAMPLE 12

N-f1-(1-Adamantvlmethvl)-2.4-dioxo-5-f(3-hvdroxv-2(R) amino)propyl]-2.3.4.5tetrahvdro-1 H-1.5-benzodiazepin-3-vn-N’-phenvlurea hydrochloride

The solution of the racemic intermediate 35 ( 0.34 g) in methanol ( 50 ) previously saturated with hydrochloric acid was stirred at 20° for 4 h; the reaction mixture was concentrated under vacuum, taken up in diethylether and

AP/P/ 94/00657

1160CH

AP.00492 crystallised from methanol/diethylether to give the racemic title compound as a white solid ( 0.175g ) M.p. >240°dec.lR: 3440-2500 (NH, OH, NH3⁺), 1697 and 1684 (C=O), cm-1; 1H-NMR: 9.20 (bs); 8.36 ( bs); 8.09 (bs); 7.74 (m); 7.46 (m);

7.33 (m); 7.21 (m); 6.90 (m); 6.90 (m); 5.69 (t); 5.41 (t); 4.92 (d); 4.21 (m); 4.045 3.82 (m); 3.82-3.56 (m); 3.40 (m); 1.79 (m); 1.64-1.08 (m).

EXAMPLE 13

N-f1-(Cyclohexylmethyl)-2.4-dioxo-5-f(2-diethylamino)ethvl1-2.3.4.5-tetrahvdro1 H-1 .S-benzodiazepin-S-vll-N’-phenylurea

Phenyl isocyanate (0.018ml) was added to a solution of the intermediate 39 (0.057g) in dry acetonitrile (2ml). The reaction mixture was stirred at 23° for 30min, then filtered to give the title compound as a white solid (0.05g). M.p. 186-188°. T.I.c. DCM-MeOH 9:1, Rf 0.8. IR:3400 (NH), 1699, 1666 and 1641 (C-O) cm-¹; ¹H.

EXAMPLE 14

N-f 1-(1-Adamantylmethyl)-2.4-dioxo-7-fluoro-5-f2-(4-morpholino)ethyll-2,3,4,5tetrahvdro-1 H-1,5-benzodiazepin-3-vll-N’-phenvlurea

Phenyl isocyanate (0.23ml) was added to a solution of intermediate 44 (0.091 g) in acetonitrile (4ml) and the mixture was stirred at 23° for 30min. The mixture was concentrated in vacuo and the residue was purified by flash chromatography (eluting with DCM) then triturated with petrol to give the title compound (0.074 g) as a white solid. T.I.c. CH-EA (1:1) Rf 0.37. IR (nujol): 3327 (NH), 1695-1660 (CO) cm-¹.

The compound of Example 14 was separated into its enantiomers(ISOMER 1 and ISOMER 2) by chiral HPLC using a (Pirkle D-DNBPGC5 column (25cm x 2cm id), flow rate 1.0ml/min., at 235nm (UV detector), and eluting with DCM-IPA 93:7 v/v isomer 1(0.048g) as a white solid, HPLC: retention time 4.4min., enantiomeric excess 100%. IR (nujol): 3327 (NH), 1695-1660 (CO) cm-¹.

isomer 2 (0.045g) as a white solid HPLC: retention time 6.0min., enantiomeric excess 96%. aD = +31.3. IR (nujol): 3327 (NH), 1695-1660 (CO) cm-¹.

EXAMPLE 15

AP/P/ 9 4 / 0 0 6 57

BAD ORIGINAL ft

1160CH

N-i1-(3-methvl-1-butvl)-2,4-dioxo-5-f2-(4-morpholino)ethyH-2.3.4.5-tetrahydro1 H-benzodiazepin-3-vn-N'-(4-chlorophenvl)urea

4-Chlorophenyl isocyanate (0.019ml) was added to a solution of intermediate 47 (0.05g) in acetonitrile (2ml). The reaction mixture was stirred at 23° for 30min, then the solvents were removed in vacuo. The residue was triturated with diethyl ether to give the title compound as a white solid (0.046g). M.p 2102°C. T.I.c. EA-MeOH (95:5), Rf 0. 53. IR: 1693-1641 (C=O) cm¹;

EXAMPLE 16

N-f2.4-Dioxo-1-(3-methvl-1-butvl)-5-f2-(4-morpholino)ethvn-2.3.4.5-tetrahydro1/-/-1.5-benzodiazepin-3-vl1-N'-(4-trifluromethvl)phenylurea

4-Trifluoromethylphenyl isocyanate (0.021ml), was added to a solution of intermediate 47 (0.090 g) in dry acetonitrile (2 mL).The solid was filtered off,washed with diethyle to give the title compound(0.06 g) as a white solid. T.I.c. (EA/MeOH 19:1) Rf=0.65,M.p.: 208-210 °.

EXAMPLE 17

N-f2.4-Dioxo-1-f2-(hexamethyleneimino)ethvn-5-phenyl-2.3.4.5-tetrahydro-1Hbenzodiazepin-S-vD-N'-O-tolvOurea

3-Tolyl isocyanate (0.040ml) was added to a solution of intermediate 50 (0.154g) in acetonitrile (7ml). The reaction mixture was stirred at 23° for 10min, then the solid was filtered off and oven dried to give the title compound as a white solid (0.130g). M.p 120-1°. T.I.c. EA-MeOH (95:5), Rf 0. 53. IR: 1703, 1643 (C=O) cm-¹;

EXAMPLE 18 (-)f1 -(1 -Adamantvlmethyl)-2.4-dioxo-5-[2-(4-morpholino)ethyl1-2.3.4.5tetrahvdro-1 H-1 .S-benzodiazepin-S-vD-N'-phenvIurea, hydrochloride salt

Example 11 isomer 1(089g) was dissolved in methanol (15ml), already saturated with gaseous hydrochloric acid, and the mixture was stirred at 0° for 2h. The mixture was concentrated in vacuo and coevaporated with dichloromethane then with diethyl ether. The residue was triturated with diethyl ether to give the title compound as a white solid (0.065g). M.p. 260-3°. T.I.c. (EA-MeOH 9:1) Rf 0.66. IR (CHCI3): 3300-2500 (NH); 1701, 1666 (C=O); 1599 (C=C) cm’¹. ¹H-NMR: 13.2 (b); 7.56 (m); 7.45-7.20 (m); 7.06 (m); 6.44 (bd);

AP/P/ 9 4 / 0 0 6 57

1160CH

AP.00492

5.03 (d); 4.51 (m); 4.36 (d); 4.20 (m); 3.98 (m); 3.52 (m); 3.22 (d); 3.01 (m); 1.85 (m); 1.70-1.10 (m).

Pharmacy Example

Capsules or Tablets mg/dosage form

Active ingredient 0.1

Polyethyleneglycol 15.0

Lactose 52.4

Starch 30.0

Magnesium stearate ' 0.5

Silicon dioxide 1.0

Sodium Lauryl Sulphate 1.0

100.0

The active ingredient is dispersed in a suitable solvent (e.g. ethanol) together with polyethyleneglycol. The solvent is removed. The powder so obtained is blended with the other excipients. The blend can be used to fill gelatine capsules or compressed using appropriate punches. The tablets can be coated using conventional techniques and coatings.

AP/P/ 94/00657

Active ingredient 0.1

Povidone 15.4

Lactose 74.0

Hydrogenated vegetable oils 3.0

Silicon dioxide 1.0

Sodium Laauryl sulphate 1.5

Crospovidone 5.0

100.0

The active ingredient is dispersed in a suitable solvent (e.g. ethanol) together with povidone. The solution is sprayed on to lactose and the solvent removed.

1160CH

The powder obtained is blended with the other excipients. The blend is used to fill gelatine capsules or comprssed using appropriate punches. The tablet can be coated using conventional techniques and coatings.

Oral liquid

Active ingredient 70-100 micrograms/dose ethanol 5-15%

Sodium saccharinate 0.1 -1 %

Propylene glycol 10-100%

Purified water qb 100%

Pack; plastic or glass bottle or other suitable pack

Injection Formulation

Active ingredient 15 Sodium phosphate

NaOH propylene glycol water for injection

0.1-100 micrograms 1.50 mg/ml qs desired pH (range 3-9) 10-500 mg/ml qs to 0.5-10ml

Pack: glass (ampules) with a rubber stopper (vials, syringes) and a plastic/metal overseal (vials only) or other suitable pack . An inert gas atmosphere (for example nitrogen) may be introduced into head space of container.

AP/P/ 9 4 / 0 0 6 57

CCK - Receptor Binding

The binding affinity of the compounds of the invention for the CCK-A receptor (Pancreas Assay) and CCK-B receptor (guinea pig cortex assay) was determined using the procedure of G Dal Forno et al J. Pharmacol. Exp & Ther.

261 -1056-1063. The pKi values determined with respresentative compounds of

1160CH

AP.00492

Compound Ex No _pKi

1	CCK-A 6.17	CCK-B 8.54
2	5.69	8.17
3	5.48	8.33
4	5.29	8.10
5	5.08	8.13
6	5.39	8.80
7	5.49	8.89
8	4.69	8.44
9	5.69	9.24
10	5.02	8.98
11 (isomer 1)	5.69	9.67
12	5.04	8.18
13	5.82	8.99
14	5.88	8.36
15	5.99	8.71
16	5.83	8.44
17	5.08	8.7

AP/P/ 94/00657

The compounds of the invention are essentially non-toxic and therapeutically useful doses. Thus for example no untoward effects were observed when the compound of Example 18 or the corresponding free base thereof (Example 11) was given orally to mice and rats at doses at which the compounds exhibit anxiolytic activity.

Claims (3)

1. (1) Compounds of the general formula (I) wherein

   R¹ represents a phenyl, C3_7cycloalkyl, C7.11 bridgedcycloalkyl or Ci_galkyl group which alkyl group may be substituted by a hydroxy, phenyl, Ci. galkoxycarbonyl, C3_7cycloalkyl, or C7.11 bridgedcycloalkyl group;

   R² represents a phenyl group optionally substituted by 1 or 2 substituents selected from, halogen, C1-4alkyl, CMalkylthio, cyano, nitro, trifluoromethyl, trifluoromethoxy, (CH
2. 2)nR⁴ or O(CH2)pR⁴ wherein R⁴ represents hydroxy, C_{v 4}alkoxy, CO₂R⁵ or NR⁶R⁷; n is zero or 1; p is an integer from 1 to 4;

   R³ represents the group AlkNR®R⁹;

   R⁵ represents hydrogen or C^alkyl;

   R⁶ represents hydrogen or C^alkyl;

   R⁷ represents hydrogen, C^alkyl, acyl, or C^alkyl substituted by one or more hydroxy, carboxyl and/or amino groups or R⁶ and R⁷ together with the nitrogen atom to which they are attached form a 5-7 saturated heterocyclic ring which contain an additional heteroatom selected from oxygen, sulphur or nitrogen and/or may be substituted by 1 or 2 C₁_₄alkyl or hydroxy groups.

   R⁸ and R⁹ independently represent hydrogen, C_1-4alkyl or C^alkyl substituted by one or more hydroxy, carboxyl and/or amino groups or R⁸ and R⁹ together with the nitrogen atom to which they are attached represent a 5-7 saturated heterocyclic ring which may contain an additional heteroatom selected from oxygen, sulphur or nitrogen and/or may be substituted by 1 or 2 Chalky I or hydroxy groups; Aik represents a straight or branched C^alkylene chain

   AP/P/ 94/00657

   1160CH

   AP.00492

   R¹⁰ represents hydrogen or a halogen atom; m is zero, 1 or 2;

   X is oxygen or NH; and pharmaceutically acceptable salts and or metabolically labile esters thereof.

   5 (2) Compounds as claimed in Claim 1 wherein X is NH.
3. (3) Compounds as claimed in Claim 1 or Claim 2 wherein R¹ represents a phenyl, cyclohexylmethyl, 3-methylbutyl or 1 -adamantylmethyl group.

   10 4. Compound as claimed in any of Claims 1 to 3 wherein R¹ represents 1adamantylmethyl.

   5. Compounds as claimed in any of Claims 1 to 4 wherein R² represents a phenyl group or a phenyl group substituted by one or two groups selected from

   15 fluorine, chlorine, bromine, methyl, methoxy, hydroxy, trifluoromethyl or thiomethyl.

   6. Compounds as claimed in any one of Claims 1 to 5 wherein R² represents phenyl, 3-methylphenyl, 4 fluorophenyl or 4-methoxyphenyl

   7. Compounds as claimed in any of Claims 1 to 6 wherein Aik represents ethylene, propylene or 2-hydroxymethylethylene.

   8. Compounds as claimed in any of Claims 1 to 7 wherein NR⁸R⁹ represents 25 amino, dimethylamino, diethylamino, morpholino, pyrrolidino, piperidino or hexamethyleneimino.

   9. Compounds as claimed in any one of Claims 1 to 8 wherein R³ represents morpholinoethyl, pyrrolidinoethyl, piperidinoethyl, dimethylaminoethyl,

   30 diethylaminoethyl, dimethylaminopropyl, aminopropyl, 2-hydroxymethyl-2aminoethyl

   10. Compounds as claimed in any of Claims 1 to 9 wherein R³ represents _morpholinoethyl.______

   35 __

   AP/P/ 9 4 / 0 0 6 57

   BAD ORIGINAL

   1160CH

   11. Compounds as claimed in any of Claims 1 to 10 wherein R¹⁰ represents hydrogen

   12. (-)[1-(1-Adamantylmethyl)]-2,4-dioxo-5-[2-(N-morpholino)-ethyl]-2,3,4,55 tetrahydro-1 H-1,5-benzodiazepin-3-yl}-N'-phenylurea and physiologically acceptable salts thereof.

   13. The compounds:- N-[1-(1-Adamantylmethyl)-2,4-dioxo-5-[2-(4morpholino)ethyl]-2,3,4,5-tetrahydro-1H-benzodiazepin-3-yl]-N'-phenylurea;

   f 10

   N-[1-(1-Adamantylmethyl)2,4-dioxo-5-[2-(4-morpholino)ethyl]-2,3,4,5 tetrahydro1 H-benzodiazepin-3-yl]-N¹-(4-fluorophenyl) urea.

   N-[2,4-Dioxo-1-(3-methyl-1-butyl)-5-[2-(4-morpholino)ethyl]-2,3,4,5-tetrahydro15 1H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

   N-[2,4-Dioxo-1 -(3-methyl-1 -butyl )-5-[2-(1 -piperidino)ethyl]-2,3,4,5-tetrahydro1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

   20 N-[5-[2-(Dimethylamino)ethyl]-2,4-dioxo-1 -(3-methyl-1 -butyl)-2,3,4,5-tetrahydro1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea ;

   N-[5-[2-(Dimethylamino)ethyl]-2,4-dioxo-1 -(3-methyl-1 -butyl)-2,3,4,5-tetrahydro1 H-1,5-benzodiazepin-3-yl]-N’-(4-methoxyphenyl)urea

   N-[2,4-Dioxo-1-(3-methyl-1-butyl)-5-[2-(4-morpholino)ethyl]-2,3,4,5-tetrahydro1 H-1,5-benzodiazepin-3-yl]-N'-(4-methoxyphenyl)urea;

   N-[2,4-Dioxo-1-(3-methyl-1-butyl)-5-[2-(4-morpholino)ethyl]-2,3,4,5-tetrahydro30 1 H-1,5-benzodiazepin-3-yl]-N'-(4-hydroxyphenyl)urea;

   N-[5-[2-(diethylamino)ethyl]-2,4-dioxo-1-(3-methyl-1-butyl)-]-2,3,4,5-tetrahydro1H-benzodiazepin-3-yl]-N’-phenylurea;

   AP/P/94/ 0 0 6 57

   1160CH

   AP.00492

   N-[5-[2-diethylamino)ethyl]-2,4-dioxo-1-(3-methyl-1-butyl)-]-2,3,4,5-tetrahydro1 H-benzodiazepin-3-yl]-N'-N'-(4-fluorophenyl)urea;;

   N-[(1-Adamantylmethyl)-5-[2-(dimethylamino)ethyl]-2,4-dioxo-2,3,4,5-tetrahydro5 1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

   N414¹-Adamantyl)methyl-5-[3-(dimethylamino)propyl]-2,4-dioxo-2,3,4,5tetrahydro-1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

   ( 10 N-[1-(1-Adamantylmethyl)-2,4-dioxo-5-[3-hydroxy-2(R) aminopropylJ-2,3,4,5tetrahydro-1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea hydrochloride;

   N-[1-(1-Cyclohexylmethyl-2,4-dioxo-5-[2-(diethylamino)ethyl]-2,3,4,5-tetrahydro1 H-benzodiazepin-1 H-benzodiazepin-3-yl]-N'-phenylurea;

   N-[1-(1-Adamantylmethyl)-2,4-dioxo-7-fluoro-5-[2-(4-morpholino)-ethyl]-2_l3,4,5tetrahydro-1 H-1,5-benzodiazepin-3-yl]-N'-phenylurea;

   N-[1-(3-methyl-1-butyl)]-2,4-dioxo-5-(2-(4-morpholino)ethyl)]-2,3,4,5-tetrahydro20 1 H-benzodiazepin-3-yl]-N'-(4-chlorophenyl)urea;

   N-[2,4-Dioxo-1-(3-methylbut-1-yl)-5-[2-(4-morpholino)ethyl]-2,3,4,5-tetrahydro1 H-1,5-benzodiazepin-3-yl]-N^,-(4-trifluromethyl)phenylurea;

   25 N-[1 -(1 -Adamantylmethyl)-2,4-dioxo-5-[2-(1 -pyrrolidino)ethyl]-2_t3,4,5-tetrahydro1H-benzodiazepin-3-yl]-N'-phenylurea

   N-[2,4-Dioxo-1-[2-(hexamethyleneimino)ethyl]-5-phenyl-2,3,4,5-tetrahydro-1Hbenzodiazepin-S-ylJ-N'-iS-tolyOurea;

   14. Compounds as claimed in any of Claims 1 to 13 for use in therapy.

   15. The use of a compound as claimed in any of Claims 1 to 13 in the manufacture of a medicament for the treatment of conditions where a

   35 modification of the effects of gastrin and or CCK is of therapeutic benefit.

   AP/P/ 94/00657

   BAD ORIGINAL

   1160CH

   16. Pharmaceutical compositions comprising a compound as claimed in any of Claims 1 to 13 in admixture with one or more physiologically acceptable carriers or excipients.

   17. A method of treatment of a mammal including man for conditions where modification of the effect of gastrin and or CCK is of therapeutic benefit comprising administration of an effective amount of a compound as claimed in any of Claims 1 to 13.

   18. A process for the preparation of compounds as defined in Claim 1 which comprises:

   (a) reacting a compound of formula (II) wherein R¹, R³ R¹⁰ and m have the meanings defined in formula (I) and Y represents the group NHCOR¹¹ wherein

   15 R¹¹ is an optionally substituted phenoxy group or a 1-imidazole group.

   AP/P/ 94/00657 with the amine (III) NH₂R² (b) reacting a compound of formula (IV)

   25 with an isocyanate of formula (V)

   O=C=NR² (V)

   1160CH

   AP.00492 or an acyl chloride (VI)

   CICO(X)R² (VI) (c) reacting a compound of formula (XI) with an alkylating agent R⁸R⁹ N Aik L wherein R⁸ R⁹ and Aik have the meanings . defined in formula (I) and L is a leaving group.

   (d) reacting a compound of formula (XIX) wherein R¹¹, R², R¹⁰, m, Aik and X have the meanings defined in formula (I) and L is a leaving group

   AP/P/ 9 4 / 0 0 6 57 with the amine R⁸a R⁹ _b NH wherein R⁸a and R⁹ _b have the meanings defined for the groups R⁸ and R⁹ respectively or R⁸a and or R⁹b are nitrogen protecting groups and thereafter if necessary or desired subjecting the resultant compound to one or more of the following operations :(i) removal of one or more protecting groups.

   (ii) conversion of one compound of the invention into another compound of the invention.

   (iii) conversion of a compound of formula (I) into an acid addition salt thereof.