AP294A

AP294A - "10-(1-Hydroxyethyl)-11-oxo-1-azatricyclo-(7.2.0.0.3.8) undec-2-ene-2-carboxylic acid derivatives".

Info

Publication number: AP294A
Application number: APAP/P/1992/000420A
Authority: AP
Inventors: Alcide Perboni; Daniele Donati; Giorgio Tarzia
Original assignee: Glaxo Spa
Priority date: 1992-08-31
Filing date: 1992-08-31
Publication date: 1993-12-28
Also published as: AP9200420A0

Abstract

R1 represents a hydrogen atom or a hydroxyl protecting group, r2 represents a hydrogen atom or a carboxyl protecting group, and r3 represents the groupn(r4)-ch=nr5 wherein r4 represents a hydrogen atom and r5 represents a hydrogen atom, and salts, metabolucally labile esters and solvates thereof and a process for their production. The compounds of formula (1)are either antibacterial agents and or useful intermediates for the preparation of antibacterial agents

Description

This invention relates to heterocyclic derivatives having antibacterial activity, to processes for their preparation, to compositions containing them, and to their use in medicine.

Thus the present invention provides compounds of the general formula (I)

in which represents a hydrogen atom or a hydroxyl protecting group;

R₂ represents a hydrogen atom, a carboxyl protecting group or a cation derived from an inorganic base or an organic base;

R₃ represents the group -N(R₄)-CH=NR«j in which R₄ represents a hydrogen atom and R$ represents a C^_₄alkyl group or R₄ represents a Cj^alkyl group and R5 represents a hydrogen atom;

and salts, metabolically labile esters and solvates thereof.

Salts of compounds of formula (I) include acid addition salts of such compounds and internal salts formed with the carboxylic acid grouping (R₂ - H).

In addition to the fixed stereochemical arrangement as defined in formula (I) the molecule contains a further asymmetric carbon atom at the 9-position, and another at the 4-position. It will be appreciated that all stereoisomers including mixtures thereof arising from these additional asymmetric centres, are within the scope of the compounds of formula (I).

The compounds of formula (I) are antibacterial agents and/or of use as intermediates for the preparation of other active compounds within the general formula (I). Compounds wherein R^ represents a hydroxyl protecting group and/or wherein R₂ represents a carboxyl protecting group are in general intermediates for the preparation of other compounds of formula (I).

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- 2 Suitable hydroxyl protecting groups and carboxyl protecting groups R₂ include those which may be removed by hydrolysis under buffered conditions or under ncn-aqueous conditions.

When the group OR^ is a protected hydroxyl group this is conveniently an ether or an acyloxy group. Examples of particularly suitable ethers include those in which R| is a hydrocarbylsilyl group such as t r i a 1 k y 1 s i 1 y 1, e.g. trimethy1si 1 y1 or tbutyldimethylsilyl. When the group OR^ represents an acyloxy group then examples of suitable groups includes alkanoyl e.g. acetyl, pivaloyl; alkenoyl e.g. allylcarbonyl; aroyl e.g. p-nitrobenzoyl; alkoxycarbonyl e.g. t-butoxycarbonyl; haloalkoxycarbonyl e.g. 2,2,2trich1oroethoxycarbony1, or 1, 1, 1 -1rich1oro-2-methy1-2 propoxycarbonyl; aralkyloxycarbony1 e.g. benzyloxycarbonyl or Pnitrobenzyloxycarbonyl; or alkenyloxycarbonyl e.g. allyloxycarbonyl.

A particularly convenient protecting group Rj is tbutyldimethylsilyl.

Examples of suitable carboxyl protecting groups include arylmethyl groups such as benzyl, p-nitrobenzyl or trityl, or alkenyl groups such as allyl or substituted allyl, t-butyl, haloalkyl e.g. trichloroethyl or tria 1ky1si1y1a 1ky1 e.g. trimethylsilylethyl. Preferred protecting groups R₂ include arylmethyl e.g. benzyl or allyl.

Particularly useful compounds of formula (I) for use in medicine as antibacterial agents are those in which the group Rj represents a hydrogen atom and R₂ represents a hydrogen atom or a physiologically acceptable cation, or an internal salt thereof; or acid addition salts of compounds wherein R₂ represents a hydrogen atom. These compounds exhibit antibacterial activity against a wide range of gram positive and gram negative, aerobic and anaerobic pathogenic microorganisms.

Where R₂ is a physiologically acceptable cation, suitable cations include those of alkali metals (e.g. sodium or potassium), alkaline earth metals (e.g. calcium), amino acids (e.g. lysine and arginine) and organic bases (e.g. procaine, phenylbenzylamine, dibenzylethylenediamine, ethanolamine, diethanolamine, and N-methyl

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- 3 Where R₂ is a cation that is not physiologically acceptable then such compounds may «be useful as intermediates for the preparation and/or isolation of other compounds of the invention.

Suitable acid addition salts of compounds of formula (I) include those formed with inorganic acids such as hydrochloric acid, hydrobromic acid, sulphuric acid, and phosphoric acid, organic acids such as acetic acid, maleic acid, fumaric acid, formic acid, succinic acid, citric acid, benzoic acid and tartaric acid and organic sulphonic acids such as p-toluenesulphonic acid and methanesulphonic acid.

The general formula (I) as drawn includes at least 4 stereoisomers and mixtures thereof and these may be represented by the formulae (la, lb, lc and Id).

lc Id

The wedge shaped bond indicates that the bond is above the plane of the paper. The broken bond 11«> indicates that the bond is below the plane of the paper.

The configuration shown for the carbon atom at the 8-position in formulae la and lb is hereinafter referred to as the β configuration and in formulae lc and Id as the a configuration.

The configuration shown for the carbon at the 4 position in formulae lb and Id is hereinafter referred to as the a confirguation and in formulae la and lc as the β configuration.

In general, in the specific compounds named below, the βconfiguration at the 8-position corresponds to the S isomer and the

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fl-configuration at the 4-position to the R isomer. The a configuration at the 8-posifcion corresponds to the R isomer and the «-configuration at the 4-position corresponds to the S isomer. The assignment of the R or S configuration at the 4- and 8- positions have been made according to the rules of Cahn. Ingold and Prelog, Experientia 1956, 12, 81.

When R₄ represents a C|_₄alkyl group and examples of such groups include methyl, ethyl, propyl and butyl.

When R₅ represents a C₁_₄alkyl group examples of such groups include methyl, ethyl, propyl and butyl.

A preferred group of compounds of formula I are those in which the carbon atom at the 8- position is in the β configuration as shown in formulae la and lb above. Within this group those compounds in which the carbon atom at the 4- position is in the a configuration as shown in formula lb above are particularly preferred.

A further preferred group of compounds of the invention are those in which the group R₄ represents a methyl group and R⁵represents a hydrogen atom or R⁴ represents a hydrogen atom and R$ represents a methyl group. More particularly preferred are those compounds wherein R⁴ represents a methyl group.

A particularly preferred group of compounds of formula (I) are those in which the carbon atom at the 8- position is in the 0 configuration and and the carbon atom at the 4- position in the a configuration, R^ represents a hydrogen atom, and Rj represents a hydrogen atom or a physiologically acceptable cation and metabolically labile esters, salts and solvates thereof.

Specific preferred compounds include ( 4S, 8S, 9R, 1 OS, 12R)-4-(time thylformamidino)-10-(l-hydroxyethyl)-ll-oxo-l-azatricyclo

Q (7.2.0.0 '] undec-2-ene-2-carboxylic acid and salts thereof e.g. sodium or potassium salts or the internal salt thereof, or an acid addition salt thereof.

Compounds according to the invention not only exhibit a broad spectrum of antibacterial activity against a wide range of pathogenic microorganisms but also have a very high resistance to all fl-lactamases. Compounds of the invention are also relatively stable to renal dehydropeptidase.

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Conpounds of the invention have been found to exhibit useful levels of activity against-strains of Staphylococcus epidermidis, Staphylococcus aureus, Streptococcus faecalis, Streptococcus pneumoniae. Streptococcus pyogenes, Streptococcus agalactiae, Escherichia coli. Pseudomonas aeruginosa, Klebi siella pneumoniae, Klebsiella oxytoca, Citrobacter diversus, Citrobacter freundii, Enterobacter cloacae, Enterobacter aerogenes, Proteus mirabilxs, Proteus rettgeri, Proteus vulgaris, Morganella morganii, Serratia marcescens, Acinetobacter calcoaceticus, Branhamella catarrhali3, Haemophilus influenzae, Haemophilus parainfluenzae, Clostridium perfringens and Bacteriodes fragilis.

The compounds of the invention may therefore be used for treating a variety of diseases caused by pathogenic bacteria in human beings and animals.

Thus, according to another aspect of the present invention, we provide a compound of formula (I) for use in the therapy or prophylaxis of systemic or topical bacterial infections in a human or animal subject.

According to a further aspect of the invention we provide the use of a compound of formula (I) for the manufacture of a therapeutic agent for the treatment of systemic or topical bacterial infections in a human or animal body.

According to a yet further aspect of the invention we provide a method of treatment of the human or non-human animal body to combat bacterial infections which method comprises administering to the body an e/fective amount of a compound of formula (I).

The compounds of the invention may be formulated frsr administration in any convenient way for use in human or veterinary medicine and the invention therefore includes within its scope pharmaceutical compositions comprising a compound of the invention adapted for use in human or veterinary medicine. Such compositions may be presented for use in conventional manner with the aid of one or more suitable carriers or excipients. The compositions of the invention include those in a form especially formulated for parenteral, oral, buccal, rectal, topical, implant, ophthalmic, nasal or genito-urinary use.

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- 6 The compounds according to the invention may be formulated for use in human or veterinary medicine by injection (e.g. by intravenous bolus injection or infusion or via intramuscular, subcutaneous or intrathecal routes) and may be presented in unit dose form, in ampoules, or other unit-dose containers, or in multidose containers, if necessary with an added preservative. The compositions for injection may be in the form of suspensions, solutions, or emulsions, in oily or aqueous vehicles, and may contain formulatory agents such as suspending, stabilising, solubilising and/or dispersing agents. Alternatively the active ingredient may be in sterile powder form for reconstitution with a suitable vehicle, e.g. sterile, pyrogen-free water, before use.

The compounds of the invention may also be presented for human or veterinary use in a form suitable for oral or buccal administration, for example in the form of solutions, gels, syrups, mouth washes or suspensions, or a dry powder for constitution with water or other suitable vehicle before use, optionally with flavouring and colouring agents. Solid compositions such as tablets, capsules, lozenges, pastilles, pills, boluses, powder, pastes, granules, bullets or premix preparations may also be used. Solid and liquid compositions for oral use may be prepared according to methods well known in the art. Such compositions may also contain one or more pharmaceutically acceptable carriers and excipients which may be in solid or liquid form.

The compounds of the invention may also be administered orally in veterinary medicine in the form of a liquid drench such as a solution, suspension or dispersion of the active ingredient together with a pharmaceutically acceptable carrier or excipient.

The compounds of the invention may also, for example, be formulated as suppositories e.g. containing conventional suppository bases for use in human or veterinary medicine or as pessaries e.g. containing conventional pessary bases.

The compounds according to the invention may be formulated for topical administration, for use in human and veterinary medicine, in the form of ointments, creams, gels, lotions, shampoos, powders, (including spray powders), pessaries, tampons, sprays, dips, aerosols, drops (e.g. eye ear or nose drops) or pour-ons.

bad origin*-

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- 7 Aerosol sprays are conveniently delivered from pressurised packs, with the use “of a suitable propellant, eg dichlorodifluoromethane, trichlorofluoromethane, dichlorotetrafluoroethane, carbon dioxide or other suitable gas.

For topical administration by inhalation the compounds according to the invention may be delivered for use in human or veterinary medicine via a nebuliser.

The pharmaceutical compositions for topical administration may also contain other active ingredients such as corticosteroids or antifungals as appropriate.

The compositions may contain from 0.01-99% of the active material. For topical administration, for example, the composition will generally contain from 0.01-10%, more preferably 0.01-1% of the active material.

For systemic administration the daily dose as employed for adult human treatment will range from 5-100mg/kg body weight, preferably 10-60mg/kg body weight, which may be administered in 1 to 4 daily doses, for example, depending on the route of administration and the condition of the patient. When the composition comprises dosage units, each unit will preferably contain 200mg to lg of active ingredient.

The duration of treatment will be dictated by the rate of response rather than by arbitrary numbers of days.

The compounds of formula (I) may be prepared by reaction of a compound of formula (II) wherein Rj, R₂ and R₄ are as defined'in formula (I) .

(II) (Hi) with an amidinating agent serving to introduce the group R^ as defined in above in formula (I). Suitable amidinating agent include compounds of formula (III) wherein R$ has the meanings as defined above in formula (I) and Rg represents a leaving group such as a halogen atom, for example a chlorine or bromine atom or a C^_₄alko'xy

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- 8 group such as methoxy or ethoxy, or an optionally substituted benzyloxy group such as benayloxy or p-nitro benzyloxy, followed as necessary or desired by removal of any protecting group R| and/or Rj. The reaction is conveniently carried out in a solvent such as tetrahydrofuran, dioxane, dimethyl formamide, dimethylsulphoxide, water or mixtures thereof and at a temperature within the range Οθ30®C. It is convenient to carry out the reaction using a compound of formula (III) in which Rg is an alkoxy or optionally substituted benzyloxy group in the form of an acid addition salt thereof such as the hydrochloride salt and in this situation a suitable base such as sodium hydroxide solution is added to the reaction mixture.

Where the groups R^ and/or R₂ are hydroxyl and carboxyl protecting groups these may be removed by conventional procedures and in any order. More preferably however the hydroxyl protecting group R^ is removed prior to the removal of the carboxyl protecting group. Such removal of the protecting groups is a further feature of the invention.

The hydroxyl protecting groups may be removed by well known standard procedures such as those described in Protective Groups in Organic Chemistry, pages 46-119, Edited by J F W McOmie (Plenum Press, 1973). For example when R| is a t-butyldimethylsilyl group, this may be removed by treatment with tetrabutylammonium fluoride and acetic acid. This process is conveniently carried out in a solvent such as tetrahydrofuran. Similarly when R^ is a trichloroethoxycarbonyl group this may be removed by treatment with zinc and acetic acid.

The carboxyl protecting group R₂ may also be removed by standard processes such as those described in Protective Groups in Organic Chemistry, pages 192-210, Edited by J F W McOmie (Plenum Press 1973). For example when R₂ represents an arylmethyl group this may be removed by conventional procedures using hydrogen and a metal catalyst e.g. palladium. When the group R₂ represents an allyl or substituted allyl group then this is preferably removed by treatment with an allyl acceptor in the presence of tetrakis(triphenylphosphine) palladium and optionally in the presence of triphenylphosphine. Suitable allyl acceptors include sterically hindered amines such as tertbutylamine, cyclic secondary

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- 9 amines such as morpholine or thioraorpholine, tertiary amines such as triethylamine, aliphatic or cycloaliphatic O-dicarbonyl compounds such as acetylacetone, ethyl acetoacetate or dimedone, or alkanoic acids or alkali metal salts thereof such as acetic acid, propionic acid or 2-ethyl hexanoic acid or the potassium or sodium salt thereof .

A particularly useful allyl acceptor is dimedone.

The reaction is preferably carried out in an inert solvent such as an ether e.g. diethyl ether or tetrahydrofuran, an alkanol e.g. ethanol, an ester e.g. ethyl acetate or a halohydrocarbon e.g. methylene chloride, or mixtures thereof. The reaction is conveniently carried out in the temperature range 0θ-40° more particularly at room temperature.

Compounds of the invention in which the group R₂ is a physiologically acceptable cation may be prepared from compounds of the invention in which R₂ is hydrogen by treatment with a suitable base. Conveniently the salt is formed in solution and then if required precipitated by the addition of a non-solvent e.g. a non polar aprotic solvent. Alternatively the sodium or potassium salt may be prepared by treating a solution of a compound of formula (I) in which R₂ represents a hydrogen atom with a solution of sodium or potassium 2-ethylhexanoate in a non-polar solvent such as diethyl ether .

Compounds of formula (II) may be prepared using the processes described in EP-A-0416953A2.

Compounds of formula (II) in which the groups Rj and R₂ and R₄have the meanings defined above may be prepared by cyclisation of, a compound of formula (IV)

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-10in which the group R? is an allyloxycarbonyl group and the groups *

Rj_a and R_2a are hydroxy and carboxyl protecting groups as defined above for R| and R₂ , followed by removal of the allyloxycarbonyl grouping and if desired or necessary removal of the hydroxy and or carboxyl protecting groups.

The cyclisation of a compound of formula (IV) is conveniently carried out by heating in the presence of an organic phosphite. The reaction is preferably carried out in a solvent or mixture of solvents at a temperature within the range 60-200°. Suitable solvents include hydrocarbons with an appropriate boiling point, for example aromatic hydrocarbons, such as toluene or xylene.

Suitable organic phosphites include acyclic and cyclic trialkylphosphites, triarylphcsphites and mixed alkylarylphosphites. Particularly useful organic phosphites are the trialkylphosphites e.g. triethylphosphite or trimethylphosphite.

The allyloxycarbonyl group R-j may be removed by conventional means, for example using the conditions described above for converting an allyl ester into the corresponding carboxylic acid.

If required the hydroxyl and carboxyl protecting groups R| and or R_2a may be removed using the procedures described above.

Compounds of formula (IV) may be prepared by treating a compound of formula (V) in which the group Rq_a, R^ and R? have the meanings given above with an activated derivative of the acid (VI) in which R_2a is a protected carboxyl group as defined above.

HOOCCO₂R_2i (VI)

Suitable activated derivatives of the acid (VI) includes the corresponding acid halides e.g. acid chloride.

When the acid halide is used as the activated derivative of the acid (VI) then the reaction is preferably carried out in the presence of an acid acceptor such as a tertiary organic base for

0AD original

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- 11 APO00294 example pyridine or a trialkylamine in an aprotic solvent such as dichloromethane.

Compounds of formula (V) in which R_la R₄ and R₇ have the meanings defined above may be prepared by the processes described in EP-A-0416953A2.

Compounds of formula (V) may also be prepared from the epoxide (VII)

(VII) (VIII) wherein R|_a is a hydroxyl protecting group and Rg is a C^_^alkyl group by reaction with the amine R^Hj in a suitable solvent such as ethyl acetate followed by reaction of the resultant keto amino (VIII) with allylchloroformate in the presence of a tertiary base such as triethylamine.

The epoxide (VII) may be prepared by oxidation of the cyclohexene derivative (IX)

(ix)

The oxidation may be carried out using a suitable per acid such as metachloroperbenzoic acid in a solvent such as dichloromethane.

The cyclohexene (IX) may be prepared by treating the ketone (X) in which R^_a is a hydroxyl protecting groups and Rg is a C^ ^alkyl group

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with a strong base such as a potassium or lithium bis (trimethylsilylaraide) and then reacting the enolate ion thus formed with the chlorophosphate Cl(O)P(ORg)2 followed by hydrolysis of the N-trialkyl silyl protecting group ((Si(R^)j)).

The compounds of formula (X) may be prepared by the methods described in ΞΡ-Α-0416952Α.

The compounds of formula (III) are either known compounds or may be prepared by analogue routes. For example compounds of formula (III) in which R₆ represents a halogen atom may be prepared by treating the corresponding amide HCONHRg with the appropriate phosphorus pentahalide e.g. PClg or PBrg.

The compounds of formula (III) in which Rg represents an alkoxy or optionally substituted benzyloxy group may be prepared by conventional routes known for preparing such imidoesters. For example the compound of formula (III) in which Rg represents a halogen atom may be converted into a compound of formula (III) in which Rg is a Cj_₄alkoxy or optionally substituted benzyloxy group by reaction with the corresponding alkoxide RgO^-. Alternatively such compounds may be prepared from the amide HCONHRg, for example by reaction with the alcohol RgOH in the presence of a suitable acid chloride, or by reaction of the amide with the fluoroborate (R₆)₃O%F₄.

In any of the formulae (I) to (X) shewn above when there is an asymmetric carbon atom and no specific configuration is shewn then the formula includes all possible configurations.

Specific stereoisomers of the compounds of formula (I) as defined in formulae la, lb, lc and Id, essentially free of the other stereoisomers may be prepared by using the general processes described above starting with the appropriate stereoisomer of formula (V).

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- 13 The processes described above for preparing the compounds of formula (V) will in general “give a mixture of stereoisomers.

The individual stereoisomers of the compounds of formula (V) may be separated from each other by conventional techniques such as fractional crystallisation or more particularly by column chromatography, using for example a silica column, as illustrated in the relevant examples.

Alternatively the synthesis may be carried out starting with a mixture of 2 or more stereoisomers of formula (V) and the required specific stereoisomer separated at by conventional techniques at another stage in the synthesis. Thus the compounds may be separated by fractional crystallisation and or column chromatography.

In order that the invention may be more fully understood the following examples are given by way of illustration only.

In the Preparations and Examples, unless otherwise stated:

Melting points (m.p.) were determined on a Gallenkamp m.p. apparatus and are uncorrected. All temperatures refer to ®C.

Infrared spectra were measured in chloroform-dj solutions on a FT-IR instrument. Proton Magnetic Resonance ( 1H-NHR) spectra were recorded at 300 MHz. Chemical shifts are reported in ppm downfield (δ) from Me^Si, used as an internal standard, and are assigned as singlets (s), doublets (d), doublet of doublets (dd) or multiplets (m) .

HPLC refers to high performance liquid chromatography.

Phosphate buffer refers to an aqueous solution of monopotassium phosphate and dipotassium phosphate at a pH of 7 and a total phosphate concentration of 0.05M.

Intermediate 1 ( 3 S , 4 R ) - 1 - ( t - b u t y 1 d i me t h y 1 s i 1 y 1 ) - 4 - a c e to xy - 3 [ ( R ) - ( t butyIdimethylsilyloxy)ethyl)azetidin-2-one

To a stirred ice-cold solution of the (3S,4R)-4- acetoxy-3((R)-{1-tbutyldimethylsilyloxy)ethyl)-2-azetidinone (112g) in dichloromethane (800ml), t-butyldimethylchlorosilane (73g) and triethylamine (30ml) were added. The mixture was stirred at room temperature for 20 hr then washed with water (1 1) and brine (300ml). The organic layer was dried and evaporated to give an oil (I60g) which was dissolved

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PD058D in a mixture of cyclohexane/ethyl acetate (95/5) (1600ml) and treated with silica gel (480g). The suspension was stirred for 15 min then filtered. The solid was washed with cyclohexane/ethyl acetate (95/5: 4.81) and the solvent evaporated to give the title compound (llOg) as a pale yellow oil. (Rf =0.85 petrol/diethyl ether =2/1)

IR(CDC1₃)ν_ΐ3χ (cm^-1): 1747(C=O)

H¹-NMR (CDC1₃):6.14(d), 4.15(m), 3.07(dd), 2.03(s), 1.2(d), 0.9(s), 0.84(s), 0.22(s), 0.055(s), 0.35(3), 0.005(s)ppm.

Intermediate 2 ; 3 S , 4 R > - 1 - ( t - b u t γ 1 d i a e t h γ 1 s i 1 v 1 - 3 - [ ( R ) - 1 - ( t but^ldimethy2_LsjJ_;yJLojcyJ_etiiylJj-£--J_2J_3j^V_^oxo-cyclohe2cylJ_L®Z®Li^iilc±c.

one

Stannic chloride (35.4ml) was added dropwise to stirred acetonitrile (400ml) under nitrogen atmosphere at -40°C, a white solid formed together with white fumes which were eliminated by nitrogen flushing. The obtained suspension was allowed to rise to -10°C then a solution of 1-trimethylsilyloxycyclohexene (60.6ml) *nd compound of Intermediate (1) ( llOg) in acetonitrile ( 300ml) was added in 10 minutes. The yellow solution was stirred at 0°C for 10 min then poured into a stirred, ice-cold, mixture of a 10% aq solution of sodium hydroxide (1 1), diethyl ether (1 1) and ice (500g). The organic layer was separated, washed again with sodium hydroxide (500ml), then with a saturated solution of ammonium chloride, dried and evaporated to give a yellow solid (117.7g). The solid was dissolved at 40^C in isopropanol (300ml) cooled to room temperature, water (300ml) was added slowly under stirring to obtain a solid which was stirred at 0°C for 30 min. The solid was filtered, washed with a 1 to 1 mixture of isopropanol/water (100ml) and dried under vacuum at 40^C for 15 hr to afford the title compound (76g) as a mixture of 2'R and 2'S isomers in a ratio of 70% to 30% (the ratio between the two isomers was determined by HPLC using hexane/ethanol (99/1) as eluant) .

Intermediate 3

_bADOWG’^nAL S

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- 15 (3S,4R,<’R)-l-t-butyldiBethyl»ilyl-3-[(K)-l-(tbutyldiaethylsilyloxy)ethyI]-4-;6^,-( 1 ' diethoxyphosphinyloxycyclohex-1 *-ene) azetidin-2-one

A 1M solution of lithium bis (trimethyl silyl) amide in hexane (9ml) was added to tetrahydrofuran (15ml), the mixture was cooled to -70C under nitrogen, then the intermediate 2 (1.9g) dissolved in tetrahydrofuran (10ml) was added over lOmin. The obtained solution was stirred for 45min, then diethyl chlorophosphonate (1.4ml) was added over 2min. The reaction mixture was stirred for 30min, allowed to warm to -20C then poured into a saturated ammonium chloride solution and the resulting mixture extracted with diethyl ether. The organic layer was washed with a 5% ice-cold solution of acetic acid, aqueous solution of sodium hydrogen carbonate and brine, dried and evaporated to give a yellow oil which was purified on silica gel (rf=0.65 diethyl ether) to afford the title compound (1.8g) as a colourless oil

IR 1732(C=O), 1670(C=C)

NMR : 5.73(m), 4.2-4(m), 3.83(m), 3.02(dd), 2.68(a), 2.09(m), 1.791.45(m), 1.34(b), 1.25(d), 0;96(s), O.88(s), 0.30(s), 0.20(s), 0.087(s) and 0.066(3).

Intermediate 4 (3S,4R,6'R)-3-[(R) -1-(t-butyIdimethylsilyloxyl) ethyl-4 -[6-( 1' diethoxyphosphinyloxycyclohex-1'-eneIazetidin-2-one

Intermediate 3 (lg) was dissolved at room temperature in methanol (25ml) and treated with potassium fluoride (500mg). The reaction mixture was stirred for 30min. Then the solvent was partially evaporated under reduced pressure. The obtained thick suspension was poured into a saturated ammonium chloride solution and the resulting mixture extracted with diethyl ether. The organic layer was washed with brine, dried and evaporated to give the title compound (750ml) as a pale yellow oil (rf=0.6 ethyl acetate).

IR : 1755(C=O), 1676{C=C)

NMR : 5.99(m), 5.69{m), 4.25-4.10(m), 4.06{dd), 3.04(dd), 2.57(m), 1.9-1.5{m), 1.33(t), 1.21(d), 0.87(s) and 0.076(3).

Intermediate 5

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- 16 (3S,4R,6’R, 2'R, l*S)-3—f(R)—I — (t-butyIdimethylsilyloxy) ethyl-4 -f 6 ’ (1'-diethoxyphosphinyloxycyolohex-‘2-ene oxide] azetidin-2-one Intermediate 4 (700mg) was dissovled in dichloromethane (25ml) at OC . Sodium hydrogen carbonate (250mg), and metachloroperbenzoic acid (700mg) were added. The obtained suspension was stirred at CC for lhr, at room temperatue for lhr then poured into an ice cold 3% aqueous sodium sulphite solution. The organic J.ayer was separated and evaporated at 20C to give an oil which was dissolved in ethyl acetate and washed with a dilute ice-cold solution of sodium hydroxide, water and brine, dried and evaporated to give a yellow oil. The crude compound was purified on silica gel (rf=0.5 ethyl acetate) to afford the pure title compound (400mg).

IR : 3416(NH), 1757(C-O)

NMR : 5.91(m), 4.25(m), 4.21(dd), 4.12(m), 3.79(d), 3.08(t),

2.49(m), 2.0-1.9(m), 1.3-1.7(m), 1.65-1.45(m), 1.45-1.3(m) 1.34(mn), 1.24(d), 0.88(s), 0.087(s) and 0.081(s).

Intermediate 6 ( 3S,4R)-3-((R)-1-(t-butyIdimethvlsilyloxy) ethyl )-4-((l'S_f2*S,6'R

2'-N-allyloxycarbonyl-N-methy1 amino)-1'-oxocyclohex-6'-yl)azetIdin2-one

To a solution of Intermediate 5 (49g) of ethylacetate (500ml) with potassium carbonate (213g) at Οθ under nitrogen was added methylamine (16g, 40% water). The reaction mixture was stirred for 1 hour at οθ then the ethyl acetate was decanted and the residual solid was washed with ethyl acetate (100ml). The organic solution was washed with water (3x600ml) and brine (1x500ml) dried, concentrated in vacuo to 500ml and cooled to 0θ. To the solution allyl chloroformate (17ml) and triethylamine (22ml) were added. The reaction mixture was stirred for 30min at 0θ then washed with a saturated aqueous solution of ammonium chloride (300ml), water (2x500ml), brine (300ml) dried and evaporated in vacuo. The residue was purified by trituration at reflux in petroleum ether (250ml) to obtain the title compound as a white powder (24.9g; m.p. 159-161θ t.l.c. diethyl ether/etnylacetate 3/2 Rf=0.68).

IR max (^CDCL3> 3414, 1753, 1688 cm-1;

bad

ORIGINAL

AP Ο Ο Ο 2 9 4

PD058D

- 17 H^NMR (300 Mhz CDC1₃): 6.2(bs), S.9(m), 5.2(m), 4.6(m), 4.2(m),

4.04(3»), 3.87(dd), 3.8(m), 3.17Idd), 2.86(s), 2.26(m), 1.8-1.2(m), *

1.30(d), 0.39(5), 0.10(s), 0.09(s).

Intermediate 7 (4S,8S,9R,10S,12R)-4-(N-methylf onnamidino)-10-(I-hydroxyethyl) -11oxo-l-azatricyclof 7,2.0.0^.θ)undec-2-ene-2-carboxylic acid

The title compound was prepared from intermediate 6 as described in EP-A-04169S3A2.

Example 1 ( 4S, 8S,9R,IOS,12R)-4-(N-methylformamidino)-10-(l-hydroxyethyl)-l1o oxo-l-azatricyclof 7.2.0.0^J. ]undec-2-ene-2-carboxylie acid (4S,8S,9R,10S,12R)-4-methylamino-10-(1-hydroxyethyl)-11-oxo-lo azatricycloj7.2.0.0. ]undec-2-ene-2-carboxylie acid (83mg) was dissolved in tetrahydrofuran (15ml) and 75ml of 0.05H, pH=7 phosphate buffer. The solution was stirred at 50C and the pH maintained at 8.5 by adding IN sodium hydroxide solution whilst benzylfonnamidate hydrochloride (600mg) was added over 45min. The tetrahydrofuran was evaporated in vacuo, the agueous solution was freeze dried and the residue purified by HPKC (Lichrosorb C18, 10μ, CH-jCN/H₂O=1 /9) to give the title compound (43mg).

^IR:Vmax (Nujol) 1761 cm’¹;

¹H-NMR ό (D₂O-Acetone): 7.76(s), 7.62(s), 5.18(m), 5.30(m), 4.24.0(m), 3.34(dd), 3.0-2.8(m+s), 2.2(m), 1.9-1.2(m) and 1.11(d) ppm.

Example 2 (4S,8S,9R,IOS,12R)-4-[(3*-methyl)fonnamidino)-10-(1-hydroxyethyl)Ί R

1l-oxo-l-azatricyclo[7.2.0.0 . )undec-2-ene-2-carboxylic acid (4S,8S,9R,10S,12R)-4-amino)-10-(1-hydroxyethyl)-11-oxo-l3 8 azatricyclo[7 . 2.0.0 . )undec-2-ene-2-carboxylic acid (31mg) was dissolved at 10° in a mixture of 0.05M phosphate buffer solution (34.4ml) and tetrahydrofuran (15.6ml) and the pH adjusted to 8.5 with IN s c d i u in hydroxide solution. p-Nitrobenzvl N-methvl formimidate hydrochloride (260mg) was added in portions over Smins to the stirred solution and the pH ci thr react»on mixture was maintained at pH 8.5 by tne addition of IN sodium hydroxide

BAD ORIGINAL ft υ λ

PDO58D

- 18 solution. The reaction mixture was stirred for 30rain at 10θ and the *

pH of the solution was adjusted to pH 7.5 by the addition of IN hydrochloric acid. The solution was washed with diethyl ether (2 x 100ml) and freeze dried. The crude solid was purified by HPLC (lichrosorb C18, S10) using acetonitrile/water 7/93 as eluant to obtain the title compound (14mg, rt = l.Smin)

IR (nujul V_max cm-1: 1589 (C=C. C=N), 1701 (C=O), 1763 (C=O 3lactam); 1H-NMR (300 MHz, DjO): 7.59 (s), 5.28(m), 4.98(m), 4.08(m), 3.97(dd), 3.28(dd), 2.93(s), 2.95-2.8(m), 1.88(m), 1.84-1.5(ra),

1.38-1.2(m), 1.11(d).

Pharmacy Example

Dry Powder for Injection

Active ingredient (Compound of Example 1) 538mg per vial.

Fill sterile vials with the sterile active ingredient. Purge the vial head space with sterile nitrogen; close the vials using rubber plugs and metal overseals (applied by crimping). The product may be constituted by dissolving in Water for Injection (10ml) or other suitable sterile vehicle for injection shortly before administration.

The antibacterial activity of the compounds of the invention may be readily determined using conventional test procedures. For example the antibacterial activity of the compounds of the invention was determined using a standard microtiter broth serial dilution test.

In this test the broth was incubated with approximately 10⁵ colony forming units of the test organism and incubated at 35° for 18 hours in the presence of test compound. Results obtained using the test procedure are given in the table below and are expressed as minimum inhibitory concentrations (MIC) in micrograms/ml.

Organism Test Compound MIC jig/ml 1 2

S aureus 663E 0.1 0.1 /£» ---AP Ο Ο Ο 29 4

PD058D

S faecalis	850E	1		16
E coli	1852E	• 0.5		2
E coli TEM1	1919E	1		1
E cloacae	3647	1		4
P aeruginosa	1911E	8		16
C perfigens	615E	0.1		0.5
B fragilis	2O17E	0.2		0.5
Test compound 1	is the	compound of	Example	1
Test compound 2	is the	compound of	Example	2

The compounds of the invention are essentially non-toxic at therapeutically useful doses. For example no adverse effects were observed when the compound of Example 1 was administered to the mouse and rat at a dose of 500mg/kg intravenously.

Claims

PD058D - 20 - Claims

1. Compounds of the general formula (I) R. O V H H A CH^^S- u/ X 1 M (1) — N—-L CT CO; A ;

in which

R| represents a hydrogen atom or a hydroxyl protecting group;

R₂ represents a hydrogen atom or a carboxyl protecting group;

and

Rj represents the group -N(R₄)-CH=NR5 in which R₄ represents a hydrogen atom and R₅ represents a C^_₄alkyl group or R₄ represents a C₁_₄alkyl group and R₅ represents a hydrogen atom;

and salts, metabolically labile esters and solvates thereof.
2. Compounds as claimed in Claims 1 wherein R^ and R₂ represent hydrogen atoms and physiologically acceptable salts, metabolically labile esters and solvates thereof.
3. Compounds as claimed in Claim 1 or Claim 2 wherein R₄represents a methyl group and R^ represents a hydrogen atom or R₄represents a hydrogen atom and represents a methyl group.
4. Compounds as claimed in any of Claims 1 to 3 wherein R₄represents a methyl group and represents a hydrogen atom.
5. Compounds as claimed in any of Claims 1 to 4 wherein represents a hydrogen atom, R₂ represents a hydrogen atom or a physiologically acceptable cation, or an internal salt thereof.
6. Compounds as claimed in any of Claims 1 to 5 having the configuration badofu??^.

AP Ο Ο Ο 2 9 4

PDO58O
7 . (4S,8S,9R,1OS,12R)-4-(N-methyIformamidino)-10-(l-hydroxyethyl) T fl

11-oxo-1-azatricyclof 7..2.0.0. °]undec-2-ene-2-carboxylie acid and physiologically acceptable salts, metabolically labile esters and solvates thereof.
8. A process for the preparation of compounds of general formula (I) as defined in Claim 1 which comprises reacting a compound of general formula (II) (ID where R^, R₂ and R₄ are as defined in Claim 1 with an amidinating agent serving to introduce the group Rj as defined in Claim 1, and thereafter if necessary or desired, subjecting the resulting compound to one or more of the following operations.

(a) removal of one or more of the protecting groups or (b) conversion of a compound in which R₂ is a hydrogen atom or a carboxyl protecting group to a corresponding salt, metabolically labile ester or solvate.
9. A process as claimed in Claim 8 wherein the amidinating agent serving to introduce the group R₂ as defined in Claim 1 is a compound of formula (III) r₆ch=nr₅ wherein Rg is as defined in Claim 1 and Rg represents a leaving group.
10. A compound claimed in any of Claim 2 to 7 for use in the therapy or prophylaxis of systemic or topical bacterial infections in a human or animal subject.

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- 22
11. The use of a compound a· claimed in any of Claims 2 to 7 in the manufacture of a therapeutic agent for the treatment or prophylaxis of systemic or topical bacterial infections in a human or animal body .

. I / I '
12./ A method of treatment of a human or non-human body to combat

V / 'Bacterial in fections comprising administration to said body of an effective amount of a compound as claimed in any of Claim 2 to 7
13. Pharmaceutical compositions comprising a compound as claimed in any of Claim 2 to 7 in admixture with one or more physiologically acceptable carriers on excipients.