US5557097A - Cytological system autofocus integrity checking apparatus - Google Patents
Cytological system autofocus integrity checking apparatus Download PDFInfo
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- US5557097A US5557097A US08/309,130 US30913094A US5557097A US 5557097 A US5557097 A US 5557097A US 30913094 A US30913094 A US 30913094A US 5557097 A US5557097 A US 5557097A
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N7/00—Television systems
- H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
- H04N7/183—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast for receiving images from a single remote source
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
- G02B21/244—Devices for focusing using image analysis techniques
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B7/00—Mountings, adjusting means, or light-tight connections, for optical elements
- G02B7/28—Systems for automatic generation of focusing signals
- G02B7/36—Systems for automatic generation of focusing signals using image sharpness techniques, e.g. image processing techniques for generating autofocus signals
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N7/00—Television systems
- H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
-
- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N7/00—Television systems
- H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
- H04N7/181—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast for receiving images from a plurality of remote sources
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N7/00—Television systems
- H04N7/18—Closed-circuit television [CCTV] systems, i.e. systems in which the video signal is not broadcast
- H04N7/188—Capturing isolated or intermittent images triggered by the occurrence of a predetermined event, e.g. an object reaching a predetermined position
Abstract
Description
TABLE 1 __________________________________________________________________________ Intensity Histogram of Entire Field For Focus + Camera Int # Int # Int # Int # Int # Int # Int # Int # __________________________________________________________________________ 0: 0 32: 0 64: 0 96: 0 128: 0 160: 0 192: 0 224: 2 1: 0 33: 0 65: 0 97: 0 129: 0 161: 0 193: 0 225: 7 2: 0 34: 0 66: 0 98: 0 130: 0 162: 0 194: 0 226: 213 3: 0 35: 0 67: 0 99: 0 131: 0 163: 0 195: 0 227: 1424 4: 0 36: 0 68: 0 100: 0 132: 0 164: 0 196: 0 228: 7803 5: 0 37: 0 69: 0 101: 0 133: 0 165: 0 197: 0 229: 47942 6: 0 38: 0 70: 0 102: 0 134: 0 166: 0 198: 0 230: 60366 7: 0 39: 0 71: 0 103: 0 135: 0 167: 0 199: 0 231: 74350 8: 0 40: 0 72: 0 104: 0 136: 0 168: 0 200: 0 232: 51837 9: 0 41: 0 73: 0 105: 0 137: 0 169: 0 201: 0 233: 15630 10: 0 42: 0 74: 0 106: 0 138: 0 170: 0 202: 0 234: 2231 11: 0 43: 0 75: 0 107: 0 139: 0 171: 0 203: 0 235: 317 12: 0 44: 0 76: 0 108: 0 140: 0 172: 0 204: 0 236: 22 13: 0 45: 0 77: 0 109: 0 141: 0 173: 0 205: 0 237: 0 14: 0 46: 0 78: 0 110: 0 142: 0 174: 0 206: 0 238: 0 15: 0 47: 0 79: 0 111: 0 143: 0 175: 0 207: 0 239: 0 16: 0 48: 0 80: 0 112: 0 144: 0 176: 0 208: 0 240: 0 17: 0 49: 0 81: 0 113: 0 145: 0 177: 0 209: 0 241: 0 18: 0 50: 0 82: 0 114: 0 146: 0 178: 0 210: 0 242: 0 19: 0 51: 0 83: 0 115: 0 147: 0 179: 0 211: 0 243: 0 20: 0 52: 0 84: 0 116: 0 148: 0 180: 0 212: 0 244: 0 21: 0 53: 0 85: 0 117: 0 149: 0 181: 0 213: 0 245: 0 22: 0 54: 0 86: 0 118: 0 150: 0 182: 0 214: 0 246: 0 23: 0 55: 0 87: 0 119: 0 151: 0 183: 0 215: 0 247: 0 24: 0 56: 0 88: 0 120: 0 152: 0 184: 0 216: 0 248: 0 25: 0 57: 0 89: 0 121: 0 153: 0 185: 0 217: 0 249: 0 26: 0 58: 0 90: 0 122: 0 154: 0 186: 0 218: 0 250: 0 27: 0 59: 0 91: 0 123: 0 153: 0 187: 0 219: 0 251: 0 28: 0 60: 0 92: 0 124: 0 156: 0 188: 0 220: 0 252: 0 29: 0 61: 0 93: 0 125: 0 157: 0 189: 0 221: 0 253: 0 30: 0 62: 0 94: 0 126: 0 158: 0 190: 0 222: 0 254: 0 31: 0 63: 0 95: 0 127: 0 159: 0 191: 0 223: 0 255: 0 __________________________________________________________________________
TABLE 2 __________________________________________________________________________ Intensity Histogram of Entire Field For Focus (-) Camera Int # Int # Int # Int # Int # Int # Int # Int # __________________________________________________________________________ 0: 0 32: 0 64: 0 96: 0 128: 0 160: 0 192: 0 224: 0 1: 0 33: 0 65: 0 97: 0 129: 0 161: 0 193: 0 225: 0 2: 0 34: 0 66: 0 98: 0 130: 0 162: 0 194: 0 226: 0 3: 0 35: 0 67: 0 99: 0 131: 0 163: 0 195: 0 227: 0 4: 0 36: 0 68: 0 100: 0 132: 0 164: 0 196: 0 228: 1 5: 0 37: 0 69: 0 101: 0 133: 0 165: 0 197: 0 229: 0 6: 0 38: 0 70: 0 102: 0 134: 0 166: 0 198: 0 230: 214 7: 0 39: 0 71: 0 103: 0 135: 0 167: 0 199: 0 231: 404 8: 0 40: 0 72: 0 104: 0 136: 0 168: 0 200: 0 232: 6823 9: 0 41: 0 73: 0 105: 0 137: 0 169: 0 201: 0 233: 8942 10: 0 42: 0 74: 0 106: 0 138: 0 170: 0 202: 0 234: 62366 11: 0 43: 0 75: 0 107: 0 139: 0 171: 0 203: 0 235: 62350 12: 0 44: 0 76: 0 108: 0 140: 0 172: 0 204: 0 236: 61837 13: 0 45: 0 77: 0 109: 0 141: 0 173: 0 205: 0 237: 16630 14: 0 46: 0 78: 0 110: 0 142: 0 174: 0 206: 0 238: 1231 15: 0 47: 0 79: 0 111: 0 143: 0 17S: 0 207: 0 239: 217 16: 0 48: 0 80: 0 112: 0 144: 0 17G: 0 208: 0 240: 122 17: 0 49: 0 81: 0 113: 0 145: 0 177: 0 209: 0 241: 0 18: 0 50: 0 82: 0 114: 0 146: 0 178: 0 210: 0 242: 0 19: 0 51: 0 83: 0 115: 0 147: 0 179: 0 211: 0 243: 0 20: 0 52: 0 84: 0 116: 0 148: 0 180: 0 212: 0 244: 0 21: 0 53: 0 85: 0 117: 0 149: 0 181: 0 213: 0 245: 0 22: 0 54: 0 86: 0 118: 0 150: 0 182: 0 214: 0 246: 0 23: 0 55: 0 87: 0 119: 0 151: 0 183: 0 215: 0 247: 0 24: 0 56: 0 88: 0 120: 0 152: 0 184: 0 216: 0 248: 0 25: 0 57: 0 89: 0 121: 0 153: 0 185: 0 217: 0 249: 0 26: 0 58: 0 90: 0 122: 0 154: 0 18G: 0 218: 0 250: 0 27: 0 59: 0 91: 0 123: 0 155: 0 187: 0 219: 0 251: 0 28: 0 60: 0 92: 0 124: 0 156: 0 188: 0 220: 0 252: 0 29: 0 61: 0 93: 0 125: 0 157: 0 189: 0 221: 0 253: 0 30: 0 62: 0 94: 0 126: 0 158: 0 190: 0 222: 0 254: 0 31: 0 63: 0 95: 0 127: 0 159: 0 191: 0 223: 0 255: 0 __________________________________________________________________________
TABLE 3 ______________________________________ Parameter Results and Limits Comparison for Static Field Uniformity Actual Parameter Description Value Limits ______________________________________ Mean Illumination Focus (+) 231 220 < × < 250 Coefficient of variation Focus (+) 0.6% <1.0% Mean Illumination Focus (+) 235 220 < × < 250 Coefficient of variation Focus (+) 0.7% <1.0% Ratio of Mean Illumination 0.98 >0.97 ______________________________________
I(X.sub.i)=G[(1+sin (2πTX.sub.i *f+Φ)* {1+G.sub.h (sin(πX.sub.i /(NoPixels-1))}]+0.5
TABLE 5 __________________________________________________________________________ Intensity Histogram of Entire Field For Focus + Camera Bin Min Max Bin Min Max Bin Min Max Bin Min Max __________________________________________________________________________ 0 0 12 64 110 133 128 0 34 192 0 13 1 0 12 65 110 133 129 0 34 193 0 13 2 0 12 66 110 133 130 0 33 194 0 13 3 0 12 67 110 133 131 0 33 195 0 13 4 0 12 68 110 133 132 0 32 196 0 13 5 0 12 69 110 133 133 0 32 197 0 13 6 0 12 70 110 133 134 0 31 198 0 13 7 0 12 71 110 133 135 0 31 199 0 13 8 0 12 72 110 133 136 0 30 200 0 13 9 0 12 73 108 131 137 0 30 201 0 13 10 0 12 74 106 129 138 0 29 202 0 13 11 0 12 75 104 127 139 0 29 203 0 13 12 0 12 76 102 125 140 0 28 204 0 13 13 0 12 77 100 123 141 0 28 20S 0 13 14 0 12 78 98 121 142 0 27 206 0 13 15 0 12 79 96 119 143 0 27 207 0 13 16 0 12 80 94 117 144 0 26 208 0 13 17 0 12 81 92 115 145 0 25 209 0 13 18 0 12 82 90 113 146 0 25 210 0 13 19 0 12 83 88 111 147 0 24 211 0 13 20 0 12 84 86 109 148 0 24 212 0 13 21 0 12 85 84 107 149 0 23 213 0 13 22 0 12 86 82 105 150 0 23 214 0 13 23 0 12 87 80 103 151 0 22 215 0 13 24 0 12 88 78 101 152 0 22 216 0 13 25 0 12 89 76 99 153 0 21 217 0 13 26 0 12 90 74 97 154 0 21 218 0 13 27 0 18 91 72 95 155 0 20 219 0 13 28 0 23 92 70 93 156 0 20 220 0 13 29 0 29 93 68 91 157 0 19 221 0 13 30 0 34 94 66 89 158 0 19 222 0 13 31 0 40 95 64 87 159 0 18 223 0 13 32 0 45 96 62 85 160 0 18 224 0 13 33 0 51 97 60 83 161 0 17 225 0 13 34 0 56 98 58 81 162 0 17 226 0 13 35 0 62 99 56 79 163 0 16 227 0 13 36 0 67 100 54 77 164 0 16 228 0 13 37 0 73 101 52 75 165 0 15 229 0 13 38 0 78 102 50 73 166 0 15 230 0 13 39 0 84 103 48 71 167 0 14 231 0 13 40 43 89 104 46 69 168 0 14 232 0 13 41 49 95 105 44 67 169 0 13 233 0 13 42 55 100 106 42 65 170 0 13 234 0 13 43 61 106 107 40 63 171 0 13 235 0 13 44 67 111 108 38 61 172 0 13 236 0 13 45 73 117 109 36 59 173 0 13 237 0 13 46 79 122 110 34 57 174 0 13 238 0 13 47 86 128 111 32 55 175 0 13 239 0 13 48 92 133 112 30 S3 176 0 13 240 0 13 49 98 133 113 28 51 177 0 13 241 0 13 50 104 133 114 26 49 178 0 13 242 0 13 51 109 133 115 24 47 179 0 13 243 0 13 52 110 133 116 22 45 180 0 13 244 0 13 53 110 133 117 20 43 181 0 13 245 0 13 54 110 133 118 18 41 182 0 13 246 0 13 55 110 133 119 16 39 183 0 13 247 0 13 56 110 133 120 14 38 184 0 13 248 0 13 57 110 133 121 12 38 185 0 13 249 0 13 58 110 133 122 10 37 186 0 13 250 0 13 59 110 133 123 8 37 187 0 13 251 0 13 60 110 133 124 6 36 188 0 13 252 0 13 61 110 133 125 4 36 189 0 13 253 0 13 62 110 133 126 2 35 190 0 13 254 0 13 63 110 133 127 0 35 191 0 13 255 0 13 __________________________________________________________________________
TABLE 6 ______________________________________ Focus + Camera MTF Frequency Limits Minimum MTF Minimum Maximum Center ______________________________________ 0.0 0.0 0.45 47.5 52.5 0.84 142.5 157.5 0.73 237.5 262.5 0.54 332.5 367.5 0.41 ______________________________________
TABLE 7 ______________________________________ Focus - Camera MTF Frequency Limits Minimum MTF Minimum Maximum Center ______________________________________ 0 0.0 0.45 47.5 52.5 0.84 142.5 157.5 0.73 237.5 262.5 0.54 332.5 367.5 0.41 ______________________________________
______________________________________ [FP(0) . . . FP(255)] * [Ffk(0) . . . Ffk(4)] => [XFPS(2) . . . XFPS(253)] [FM(0) . . . FM(255)] * [Ffk(0) . . . Ffk(4)] => [XFMS(2) . . . XFMS(253)] ______________________________________
______________________________________ [FPnoise(0) . . . FPnoise(255)] * [Ffk(0) . . . Ffk(4)] => [FPNX(2) . . . FPNX(253)] [FMnoise(0) . . . FMnoise(255)] * [Ffk(0) . . . Ffk(4)] => [FMNX(2) . . . FMNX(253)] ______________________________________
______________________________________ [XFPS(2) . . . XFPS(253)] - [FPNX (2) . . . FPNX(253)] = FPS[(2) . . . (253)] [XFMS(2) . . . XFMS(253)] - [FMNX(2) . . . FMNX(253)] = FMS[(2) . . . (253)] ______________________________________
f(x)=Ao/2+4Ao/π(sin kx+1/3 sin 3kx+1/5 sin 5kx+ . . . ).
TABLE 8 __________________________________________________________________________Vertical MTF 20X Vertical MTF for Three Portions of Field of View Freq Range Left Center Right min max freq mtf min freq mtf min freq mtf min __________________________________________________________________________ .0 0 0 0.951 0.839 0 0.939 0.839 0 0.937 0.839 48.0 52 49.7 0.951 0.91 49.7 0.95 0.91 49.7 0.953 0.91 144.0 156 149.1 0.924 0.87 149.1 0.925 0.87 149.1 0.928 0.87 240.0 260 248.5 0.885 0.819 248.5 0.885 0.819 248.5 0.893 0.819 336.0 364 348 0.845 0.75 348 0.84 0.75 348 0.85 0.75 432.0 468 447.4 0.799 0.66 447.4 0.798 0.66 447.4 0.803 0.66 528.0 572 546.8 0.76 0.55 550.4 0.743 0.55 550.4 0.756 0.55 624.0 676 649.8 0.675 0.449 649.8 0.683 0.449 649.8 0.698 0.449 730.0 780 749.2 0.607 0.349 749.2 0.623 0.349 749.2 0.632 0.349 816.0 884 848.7 0.545 0.250 848.7 0.542 0.259 848.7 0.549 0.259 __________________________________________________________________________
TABLE 9 __________________________________________________________________________Horizontal MTT 20X Horizontal MTF for Three Portions of Field of View Freq Range Left Center Right min max freq mtf min freq mtf min freq mtf min __________________________________________________________________________ .0 0 0 0.953 0.839 0 0.955 0.839 0 0.95 0.839 48.0 52 49.7 0.928 0.899 49.7 0.925 0.899 49.7 0.926 0.899 144.0 156 149.1 0.813 0.769 149.1 0.809 0.769 149.1 0.811 0.769 240.0 260 248.5 0.659 0.61 248.5 0.654 0.61 248.5 0.658 0.61 336.0 364 348 0.516 0.449 348 0.512 0.449 348 0.51 0.449 432.0 468 447.4 0.397 0.319 447.4 0.396 0.319 447.4 0.391 0.319 528.0 572 546.8 0.31 0.219 546.8 0.302 0.219 546.8 0.303 0.219 624.0 676 646.8 0.239 0.129 649.8 0.238 0.129 649.8 0.232 0.129 730.0 780 749.2 0.183 0 749.2 0.185 0 749.2 0.18 0 816.0 884 848.7 0.15 0 848.7 0.1 0 848.7 0.15 0 __________________________________________________________________________
Claims (4)
Priority Applications (14)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US08/309,130 US5557097A (en) | 1994-09-20 | 1994-09-20 | Cytological system autofocus integrity checking apparatus |
ES95930840T ES2123470T1 (en) | 1994-09-20 | 1995-08-16 | DEVICE FOR CHECKING THE INTEGRITY OF THE SELF-APPROACH OF A CYTOLOGICAL SYSTEM. |
JP8510882A JPH10506478A (en) | 1994-09-20 | 1995-08-16 | A device that checks the integrity of the autofocus of a cytological device |
EP95930840A EP0782715A4 (en) | 1994-09-20 | 1995-08-16 | Cytological system autofocus integrity checking apparatus |
CA002200453A CA2200453C (en) | 1994-09-20 | 1995-08-16 | Cytological system autofocus integrity checking apparatus |
AU34073/95A AU703182C (en) | 1994-09-20 | 1995-08-16 | Cytological system autofocus integrity checking apparatus |
PCT/US1995/010430 WO1996009564A1 (en) | 1994-09-20 | 1995-08-16 | Cytological system autofocus integrity checking apparatus |
DE0782715T DE782715T1 (en) | 1994-09-20 | 1995-08-16 | DEVICE FOR TESTING THE INTEGRITY OF AN AUTOFOCUS SYSTEM A CYTOLOGICAL SYSTEM |
US08/666,748 US5654535A (en) | 1994-09-20 | 1996-06-19 | Cytological system autofocus integrity checking apparatus |
US08/821,693 US5760387A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
US08/821,695 US5763871A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
US08/821,694 US5877489A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
US08/821,699 US5892218A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
GR980300045T GR980300045T1 (en) | 1994-09-20 | 1998-07-31 | Cytological system autofocus integrity checking apparatus |
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US08/309,130 US5557097A (en) | 1994-09-20 | 1994-09-20 | Cytological system autofocus integrity checking apparatus |
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US08/666,748 Division US5654535A (en) | 1994-09-20 | 1996-06-19 | Cytological system autofocus integrity checking apparatus |
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US5557097A true US5557097A (en) | 1996-09-17 |
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US08/309,130 Expired - Lifetime US5557097A (en) | 1994-09-20 | 1994-09-20 | Cytological system autofocus integrity checking apparatus |
US08/666,748 Expired - Lifetime US5654535A (en) | 1994-09-20 | 1996-06-19 | Cytological system autofocus integrity checking apparatus |
US08/821,695 Expired - Lifetime US5763871A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
US08/821,693 Expired - Lifetime US5760387A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
US08/821,699 Expired - Lifetime US5892218A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
US08/821,694 Expired - Lifetime US5877489A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
Family Applications After (5)
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US08/666,748 Expired - Lifetime US5654535A (en) | 1994-09-20 | 1996-06-19 | Cytological system autofocus integrity checking apparatus |
US08/821,695 Expired - Lifetime US5763871A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
US08/821,693 Expired - Lifetime US5760387A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
US08/821,699 Expired - Lifetime US5892218A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
US08/821,694 Expired - Lifetime US5877489A (en) | 1994-09-20 | 1997-03-20 | Cytological system autofocus integrity checking apparatus |
Country Status (8)
Country | Link |
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US (6) | US5557097A (en) |
EP (1) | EP0782715A4 (en) |
JP (1) | JPH10506478A (en) |
CA (1) | CA2200453C (en) |
DE (1) | DE782715T1 (en) |
ES (1) | ES2123470T1 (en) |
GR (1) | GR980300045T1 (en) |
WO (1) | WO1996009564A1 (en) |
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US5654535A (en) * | 1994-09-20 | 1997-08-05 | Neopath, Inc. | Cytological system autofocus integrity checking apparatus |
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Also Published As
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US5760387A (en) | 1998-06-02 |
CA2200453C (en) | 2002-03-12 |
JPH10506478A (en) | 1998-06-23 |
AU3407395A (en) | 1996-04-09 |
US5763871A (en) | 1998-06-09 |
ES2123470T1 (en) | 1999-01-16 |
GR980300045T1 (en) | 1998-07-31 |
DE782715T1 (en) | 1998-11-19 |
WO1996009564A1 (en) | 1996-03-28 |
EP0782715A1 (en) | 1997-07-09 |
CA2200453A1 (en) | 1996-03-28 |
EP0782715A4 (en) | 1999-10-20 |
US5877489A (en) | 1999-03-02 |
US5654535A (en) | 1997-08-05 |
US5892218A (en) | 1999-04-06 |
AU703182B2 (en) | 1999-03-18 |
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