WO2022009136A3 - Method of encapsulation of an active protein using electrodeposition techniques, |an antibacterial composition containing the active protein and polymer and its|use for the production of veterinary medicinal products - Google Patents
Method of encapsulation of an active protein using electrodeposition techniques, |an antibacterial composition containing the active protein and polymer and its|use for the production of veterinary medicinal products Download PDFInfo
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- WO2022009136A3 WO2022009136A3 PCT/IB2021/056130 IB2021056130W WO2022009136A3 WO 2022009136 A3 WO2022009136 A3 WO 2022009136A3 IB 2021056130 W IB2021056130 W IB 2021056130W WO 2022009136 A3 WO2022009136 A3 WO 2022009136A3
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- Prior art keywords
- active protein
- polymer
- culture
- cells
- antibacterial composition
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
- A61J3/00—Devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms
- A61J3/07—Devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms into the form of capsules or similar small containers for oral use
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
- A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/11—Encapsulated compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/81—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions involving only carbon-to-carbon unsaturated bonds
- A61K8/8129—Compositions of homopolymers or copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and at least one being terminated by an alcohol, ether, aldehydo, ketonic, acetal or ketal radical; Compositions of hydrolysed polymers or esters of unsaturated alcohols with saturated carboxylic acids; Compositions of derivatives of such polymers, e.g. polyvinylmethylether
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Birds (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Communicable Diseases (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- Developmental Biology & Embryology (AREA)
- Oncology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Textile Engineering (AREA)
- Biochemistry (AREA)
- Mechanical Engineering (AREA)
- Hematology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Dermatology (AREA)
- Toxicology (AREA)
- Virology (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Emergency Medicine (AREA)
- Medicinal Preparation (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The subject of invention is a method of encapsulating an active protein using electrodeposition techniques, characterised in that it comprises the following steps: (a) establishing a primary mesenchymal cell culture containing 2,000-5,000 source tissue cells and a serum-supplemented culture medium; (b) maintaining the cell culture established in step (a) for 280-340 hours until the culture surface is fully covered by the cultured cells; (c) obtaining a culture fluid from the above of the cultured cells; (d) purifying the culture fluid obtained in step (c) from cell debris and suspended cells by centrifuging said fluid with a force of 300 to 1200 x g; (e) transferring the upper liquid phase from above the sediment to a new vessel; (f) gently mixing the purified liquid phase obtained in step (e) with an aqueous solution of polyvinyl alcohol; (g) adding ethyl alcohol to the mixture obtained in step (f) while stirring continuously; (h) the material obtained in step (g) is deposited on the collector surface by means of electro spinning or electrospraying. Another subject of invention is an antibacterial composition containing an active protein and a polymer, characterised in that it contains ethyl alcohol, wherein the active protein is a fibrous, fully water-soluble material containing proteins released by mesenchymal cells, including cathelicidin at an amount from 270 to 1230 pg/100mg of dry weight of the composition, and the polymer is an aqueous solution of polyvinyl alcohol. Another subject of invention is an use of the antibacterial composition according to invention for the preparation of veterinary medicinal products.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PL434626 | 2020-07-10 | ||
PL434626A PL434626A1 (en) | 2020-07-10 | 2020-07-10 | Active protein encapsulation method using electrodeposition techniques, an antimicrobial composition containing the active protein and a polymer, and its use in production of veterinary medicinal products |
Publications (3)
Publication Number | Publication Date |
---|---|
WO2022009136A2 WO2022009136A2 (en) | 2022-01-13 |
WO2022009136A3 true WO2022009136A3 (en) | 2022-03-24 |
WO2022009136A4 WO2022009136A4 (en) | 2022-06-16 |
Family
ID=77519404
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2021/056130 WO2022009136A2 (en) | 2020-07-10 | 2021-07-08 | Method of encapsulation of an active protein using electrodeposition techniques, an antibacterial composition containing the active protein and a polymer, and its use for the production of veterinary medicinal products |
Country Status (2)
Country | Link |
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PL (1) | PL434626A1 (en) |
WO (1) | WO2022009136A2 (en) |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120276215A1 (en) * | 2011-04-26 | 2012-11-01 | Riordan Neil H | Therapeutic Conditioned Media |
CN106823009A (en) * | 2017-01-12 | 2017-06-13 | 广东泰宝医疗器械技术研究院有限公司 | A kind of antibacterial bone renovating bracket material with induction repair and preparation method thereof |
US20180242590A1 (en) * | 2016-09-23 | 2018-08-30 | Peter Friedman | Compositions and methods for treating citrus plants infected with bacteria and for promoting general agricultural health |
CN109172606A (en) * | 2018-11-30 | 2019-01-11 | 天津欣普赛尔生物医药科技有限公司 | A kind of sustained-release micro-spheres and preparation method of the body of excretion containing mescenchymal stem cell |
WO2019231562A1 (en) * | 2018-05-30 | 2019-12-05 | Direct Biologics Llc | A growth factor and extracellular vesicle frozen or powdered additive comprising a mesenchymal stem cell (msc) preparation and methods of use |
WO2019246014A1 (en) * | 2018-06-18 | 2019-12-26 | North Carolina State University | Stem cell biomimetic nanoparticle therapeutic agents and uses thereof |
-
2020
- 2020-07-10 PL PL434626A patent/PL434626A1/en unknown
-
2021
- 2021-07-08 WO PCT/IB2021/056130 patent/WO2022009136A2/en active Application Filing
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120276215A1 (en) * | 2011-04-26 | 2012-11-01 | Riordan Neil H | Therapeutic Conditioned Media |
US20180242590A1 (en) * | 2016-09-23 | 2018-08-30 | Peter Friedman | Compositions and methods for treating citrus plants infected with bacteria and for promoting general agricultural health |
CN106823009A (en) * | 2017-01-12 | 2017-06-13 | 广东泰宝医疗器械技术研究院有限公司 | A kind of antibacterial bone renovating bracket material with induction repair and preparation method thereof |
WO2019231562A1 (en) * | 2018-05-30 | 2019-12-05 | Direct Biologics Llc | A growth factor and extracellular vesicle frozen or powdered additive comprising a mesenchymal stem cell (msc) preparation and methods of use |
WO2019246014A1 (en) * | 2018-06-18 | 2019-12-26 | North Carolina State University | Stem cell biomimetic nanoparticle therapeutic agents and uses thereof |
CN109172606A (en) * | 2018-11-30 | 2019-01-11 | 天津欣普赛尔生物医药科技有限公司 | A kind of sustained-release micro-spheres and preparation method of the body of excretion containing mescenchymal stem cell |
Non-Patent Citations (7)
Title |
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ANNA KRASNODEMBSKAYA ET AL: "Antibacterial Effect of Human Mesenchymal Stem Cells Is Mediated in Part from Secretion of the Antimicrobial Peptide LL-37", STEM CELLS, vol. 28, no. 12, 1 December 2010 (2010-12-01), pages 2229 - 2238, XP002744868, ISSN: 1066-5099, DOI: 10.1002/stem.544 * |
FELICE BETIANA ET AL: "Electrosprayed poly(vinyl alcohol) particles: preparation and evaluation of their drug release profile : Electrosprayed PVA: preparation and evaluation of drug release profile", POLYMER INTERNATIONAL, vol. 64, no. 12, 29 July 2015 (2015-07-29), GB, pages 1722 - 1732, XP055851753, ISSN: 0959-8103, DOI: 10.1002/pi.4972 * |
JOHNSON VALERIE ET AL: "Activated Mesenchymal Stem Cells Interact with Antibiotics and Host Innate Immune Responses to Control Chronic Bacterial Infections", SCIENTIFIC REPORTS, vol. 7, no. 1, 29 August 2017 (2017-08-29), XP055851237, Retrieved from the Internet <URL:https://www.nature.com/articles/s41598-017-08311-4.pdf> DOI: 10.1038/s41598-017-08311-4 * |
MARRAZZO PASQUALE ET AL: "Exploring the roles of MSCs in infections: focus on bacterial diseases", JOURNAL OF MOLECULAR MEDICINE, SPRINGER BERLIN HEIDELBERG, BERLIN/HEIDELBERG, vol. 97, no. 4, 7 February 2019 (2019-02-07), pages 437 - 450, XP036745071, ISSN: 0946-2716, [retrieved on 20190207], DOI: 10.1007/S00109-019-01752-6 * |
SEERAM RAMAKRISHNA ET AL: "Advances in drug delivery via electrospun and electrosprayed nanomaterials", INTERNATIONAL JOURNAL OF NANOMEDICINE, 1 August 2013 (2013-08-01), pages 2997, XP055357342, DOI: 10.2147/IJN.S43575 * |
SU YAJUAN ET AL: "Nanofiber Dressings Topically Delivering Molecularly Engineered Human Cathelicidin Peptides for the Treatment of Biofilms in Chronic Wounds", MOLECULAR PHARMACEUTICS, vol. 16, no. 5, 8 April 2019 (2019-04-08), US, pages 2011 - 2020, XP055850677, ISSN: 1543-8384, Retrieved from the Internet <URL:https://pubs.acs.org/doi/pdf/10.1021/acs.molpharmaceut.8b01345> DOI: 10.1021/acs.molpharmaceut.8b01345 * |
ZHANG WANWEI ET AL: "Electrospun Nanofibres Containing Antimicrobial Plant Extracts", NANOMATERIALS, vol. 7, no. 2, 15 February 2017 (2017-02-15), pages 42, XP055811362, DOI: 10.3390/nano7020042 * |
Also Published As
Publication number | Publication date |
---|---|
PL434626A1 (en) | 2022-01-17 |
WO2022009136A4 (en) | 2022-06-16 |
WO2022009136A2 (en) | 2022-01-13 |
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