WO2021112660A1 - Kit and cylindrical device for the detection of an infectious agent - Google Patents
Kit and cylindrical device for the detection of an infectious agent Download PDFInfo
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- WO2021112660A1 WO2021112660A1 PCT/MX2020/000047 MX2020000047W WO2021112660A1 WO 2021112660 A1 WO2021112660 A1 WO 2021112660A1 MX 2020000047 W MX2020000047 W MX 2020000047W WO 2021112660 A1 WO2021112660 A1 WO 2021112660A1
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- infectious agent
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
Definitions
- the present invention is related to the detection of an infectious agent from probes with fibers or paper, and more particularly it relates to a kit and cylindrical device for the detection of an infectious agent.
- NC nitrocellulose
- W02007 / 106050 describes an ELISA test to detect flavivirus member-specific antibodies from biological samples, and is based on a competition for epitope binding on the envelope protein of captured flavivirus antigen using Anti-flavivirus IgA in the presence of positive flavivirus serum.
- document CN205679623U describes a nitrocellulose fiber covered with colloidal gold and a dengue virus antigen detection kit from blood samples.
- Another object of the present invention is to provide a kit for the detection of an infectious agent that uses a cylindrical device to provide a greater contact area and thus reduce operating costs.
- kit and cylindrical device for the detection of an infectious agent in accordance with the present invention.
- a kit has been invented for the detection of an infectious agent with a cylindrical device, probe type, which allows a detection fiber to be submerged vertically, without using adhesives, to double its contact area and thus obtain a high diagnosis. sensitivity and lower operating cost.
- a first aspect of the present invention is a device for the detection of an infectious agent characterized in that it comprises; i) a female part which in turn comprises at least one slit; and ii) a male piece that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female piece, and in this way the two pieces can be joined allowing the fastening of a detection fiber vertically at one of its ends, where the device is shaped such that it can be coupled with the wells of a common tray used in tests for the detection of an infectious agent.
- a second aspect of the present invention is a kit for the detection of an infectious agent characterized in that it comprises: a) at least one detection fiber which in turn comprises a predetermined antibody for the detection of a predetermined infectious agent; b) at least one device for detecting an infectious agent characterized in that it comprises: i) a female part which in turn comprises at least one slit; and i) a male part that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female part, and in this way the two parts can be joined allowing the fastening of the detection fiber vertically at one of its ends; and c) a microplate that in turn comprises a plurality of wells and a solution within each well that potentially comprises a biomarker related to a predetermined infectious agent, wherein each device is coupled with one of the wells of the plurality of wells allowing the detection fiber is immersed in the solution.
- Figure 1 shows a perspective view of an embodiment of an infectious agent detection device (1100) in accordance with the principles of the present invention.
- Figure 2 shows a perspective view of one embodiment of the device for detecting an infectious agent (1100) shown in Figure 1.
- Figure 3 shows a side view of an embodiment of a kit for the detection of an infectious agent (1000) in accordance with the principles of the present invention.
- Figure 4 shows a perspective photograph of an embodiment of a kit for the detection of an infectious agent (1000) in accordance with the principles of the present invention.
- a first aspect of the present invention is a device for the detection of an infectious agent characterized in that it comprises: i) a female part which in turn comprises at least one slit; and ii) a male piece that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female piece, and in this way the two pieces can be joined allowing the fastening of a detection fiber vertically at one of its ends, where the device is shaped such that it can be coupled with the wells of a common tray used in tests for the detection of an infectious agent.
- the infectious agent to be detected is related to a tropical disease, more preferably to schistosomiasis, onchocerciasis, lymphatic filariasis, leishmaniasis, leprosy, dengue and severe dengue, yaws, echinococcosis, taeniasis and cysticercosis, rabies, Chagas disease, Buruli ulcer, human African trypanosomiasis, vector-borne diseases, dracunculiasis.
- the female part has a half-cylinder shape.
- said female part preferably comprises two slits.
- the male part has a half-cylinder shape.
- said male part preferably comprises two extensions.
- both are obtained by 3D printing or injection molding.
- said cylindrical device When joining both pieces to form a cylindrical shaped device, said cylindrical device preferably has a diameter of 5 mm.
- the detection fiber is selected from electrospun polymer fibers.
- a second aspect of the present invention is a kit for the detection of an infectious agent characterized in that it comprises; a) at least one detection fiber which in turn comprises a predetermined antibody for the detection of a predetermined infectious agent; b) at least one device for detecting an infectious agent characterized in that it comprises: i) a female part which in turn comprises at least one slit; and i) a male part that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female part, and in this way the two parts can be joined allowing the fastening of the detection fiber vertically at one of its ends; and c) a microplate that in turn comprises a plurality of wells and a solution within each well that potentially comprises a biomarker related to a predetermined infectious agent, wherein each device is coupled with one of the wells of the plurality of wells allowing the detection fiber is immersed in the solution.
- the proposed kit represents a tool in the early diagnosis of an infectious agent that has an operation similar to that of a conventional enzyme-linked immunosorbent assay (ELISA), making it convenient for the laboratory technician who performs ELISA on a regular basis.
- ELISA enzyme-linked immunosorbent assay
- the infectious agent to be detected is a tropical disease, more preferably it is selected from schistosomiasis, onchocerciasis, lymphatic filariasis, leishmaniasis, leprosy, dengue and severe dengue, yaws, echinococcosis, taeniasis and cysticercosis, rabies, Chagas disease, Buruli ulcer, human African trypanosomiasis, vector-borne diseases, dracunculiasis.
- the infectious agent is the dengue virus, which is preferably detected through the NS1-type protein, characteristic of the dengue virus and which serves as a biomarker.
- the detection fiber is selected from electrospun polymer fibers.
- the predetermined antibody is selected from primary and / or secondary antibodies, and more specifically the primary antibody is the NS1 glycoprotein against dengue virus and the secondary antibody is the goat anti-dengue heavy chain. mouse IgG2a.
- the female part has a half-cylinder shape.
- said female part preferably comprises two slits
- the male part has a half-cylinder shape.
- said male part preferably comprises two extensions.
- both are obtained by 3D printing or injection molding.
- the plurality of wells comprise
- the detection fibers can be submerged in a vertical position, which provides an effective interaction with both sides of said fiber, in addition, the present invention makes the application of any type of glue unnecessary, which, in turn, it minimizes the risk of protein deactivation when it comes into contact with adhesive materials.
- the device (1100) comprises a female part (1110) with two slits (lili) and a male part (1120) with 2 extensions (1121), in addition a detection fiber (1200) is shown .
- FIG 2 it shows a perspective view of an assembly modality of the device for the detection of an infectious agent (1100) shown in Figure 1.
- the two extensions ( 1121) of the male part (1120) fit with the two slits (lili) of the female part (1110), in such a way that they hold the detection fiber (1200)
- the kit (1000) comprises a plurality of devices for the detection of an infectious agent (1100); a microplate (1300) with a plurality of wells (1310); and a plurality of detection fibers (1200), where each of the devices (1100) are coupled in each of the wells (1310, in such a way that the detection fiber (1200) is submerged vertically in a solution (1311) potentially containing an infectious agent.
- FIG. 4 shows a perspective photograph of an embodiment of a kit for the detection of an infectious agent (1000) in accordance with the principles of the present invention.
- the kit (1000) comprises a device (1100) with a detection fiber (1200) submerged vertically.
- the female and male parts of the cylindrical device were manufactured using a Fortus® 400 me FDM system in ABS-M30 plastic with a total dimension of 5 mm, so that it can be inserted into one of the plurality of wells of a microplate.
- the print scheme was designed with SolidWorks® and one hundred pairs of parts were printed for the development of each 96-well microplate.
- a detection fiber obtained by electrospinning, was placed between a female part and a male part of the cylindrical device, and the above was done with the remaining 95 pairs and thus obtain 96 cylindrical devices, where each one holds a sense fiber.
- each of the 96 cylindrical devices was mounted in each of the 96 wells on the lid of a common microplate.
- An assay was performed to prepare dengue virus NS1 protein solutions and antibody solutions according to the present invention, with the following steps: a. Prepare a stock solution of phosphate buffered saline "PBS" (10X) by adding 161.2 g of NaCl, 4.4 g of KCI, 24 g of Na2HPC ⁇ 4 and 4 g of KH2PO4 to 2,000 mL of distilled water. b. use dilute phosphate saline buffer (IX) as wash buffer, which is used after coating samples with NS1 protein solution. c.
- PBS phosphate buffered saline
- IX dilute phosphate saline buffer
- a coating buffer for the preparation of the NS1 protein solution, with 100 mL of distilled water, 2 mL of 0.2 M sodium carbonate (feCCh) and 23 mL of 0.2 M sodium bicarbonate (NaHCC); d. preparing a blocking buffer to reduce the possibility of non-specific binding, with 3% bovine serum albumin "BSA" in phosphate buffered saline (IX); and.
- BSA bovine serum albumin
- An assay was performed to make the diagnosis of dengue through the detection of the biomarker NS1 in accordance with the present invention, with the following steps: a. Wash 3 times, every 5 minutes, each of the plurality of wells of a tray with 200 ⁇ l per well of phosphate buffer saline (IX) at room temperature, using a shaker with a stirring speed of 1000 rpm; b. incubate solutions of NS1 at concentrations of: 5, 50, 5xl0 2 , 5xl0 3 , 5xl0 4 and 5x10 s Pg / mL at 4 ° C for 8 hours, for each concentration 12 positive and 8 negative replicates were made, the negative replicates were prepared in the absence of the NS1 protein; c.
- each of the plurality of wells with 150 ⁇ l of the NS1 solutions from the previous step; d. Block with the lid, assembled in Example 1, each of the plurality of wells by adding 200 pL of blocking buffer to each well. Incubation was carried out at 37 ° C for 1 hour; and. prepare a 1: 10,000 stock antibody solution in dilution buffer, each well received 150 pL of stock solution and was blocked and incubated for 2 hours at 37 ° C); F. wash 3 times, every 5 minutes, with 200 ⁇ l per well of Tween-20 solution (previous example) at room temperature, using a shaker with a stirring speed of 1000 rpm; g.
- kit and cylindrical device for the detection of an infectious agent has been designed for the early diagnosis of a tropical disease, and it will be evident to any expert in the field that the kit modalities and cylindrical device for the detection of an infectious agent as described above and illustrated in the accompanying drawings, are only illustrative but not limiting of the present invention, since numerous changes of consideration in its details are possible without departing from the scope of the invention. Therefore, the present invention should not be construed as restricted except as required by the prior art and for the scope of the appended claims.
Abstract
In a first aspect, the present invention relates to a device for the detection of an infectious agent, characterised in that it comprises: (i) a female piece comprising at least one indentation; and (ii) a male piece. In a second aspect, the invention relates to a kit for the detection of an infectious agent, characterised that it comprises: (a) at least one detection fibre comprising a predetermined antibody for the detection of a predetermined infectious agent; (b) at least one device for the detection of an infectious agent; and a microplate comprising a plurality of wells and a solution in each well, which potentially comprises a biomarker related to a predetermined infectious agent, wherein each device is coupled to one of the wells of the plurality of wells, allowing the detection fibre to be submerged in the solution.
Description
KIT Y DISPOSITIVO CILINDRICO PARA LA DETECCIÓN DE UN AGENTE INFECCIOSO. KIT AND CYLINDRICAL DEVICE FOR THE DETECTION OF AN INFECTIOUS AGENT.
CAMPO DE LA INVENCIÓN FIELD OF THE INVENTION
La presente invención está relacionada con la detección de un agente infeccioso a partir de sondas con fibras o papel, y más particularmente está relacionada con un kit y dispositivo cilindrico para la detección de un agente infeccioso. The present invention is related to the detection of an infectious agent from probes with fibers or paper, and more particularly it relates to a kit and cylindrical device for the detection of an infectious agent.
ANTECEDENTES DE LA INVENCIÓN BACKGROUND OF THE INVENTION
Se han diseñado diferentes clases de ensayos biológicos para la detección de un agente infeccioso a base de papel o fibras, incluidas las varillas de medición, los ensayos de flujo lateral (LFA), los dispositivos analíticos basados en papel (pPAD) y la placa de micropocillos. Dichas plataformas poseen varios beneficios como lo es la rapidez y costos, así como que son ligeras y se necesita poca cantidad de volumen de muestra. Sin embargo, poseen limitaciones como sensibilidad de detección insuficiente, inestabilidad del material (papel o fibra), necesidad de tratamiento en la superficie debido a la naturaleza inerte del papel o fibra y poco tiempo de vida útil después de la activación de la superficie. Different classes of biological assays have been designed for the detection of a paper- or fiber-based infectious agent, including dipsticks, lateral flow assays (LFA), paper-based analytical devices (pPADs), and plate. microwells. These platforms have several benefits such as speed and costs, as well as being light and requiring a small amount of sample volume. However, they have limitations such as insufficient detection sensitivity, instability of the material (paper or fiber), the need for surface treatment due to the inert nature of the paper or fiber, and a short shelf life after surface activation.
Además, la mayoría de los ensayos basados en papel utilizan nitrocelulosa (NC) disponible en el mercado. No obstante, no existe un gran control sobre las propiedades de dicho papel, ya que la calidad del papel puede variar de un fabricante a otro y es conocido que la nitrocelulosa tiene un WCA de 21° a 67°, este nivel de hidrofilia puede inducir una mayor probabilidad de una señal de falso positivo. Por esa razón, las plataformas analíticas en papel generalmente sufren de sensibilidad insuficiente. Por lo tanto, es necesario desarrollar un papel con una morfología de superficie adaptada, propiedades de superficie y humectabilidad ajustada para fines específicos. In addition, most paper-based assays use commercially available nitrocellulose (NC). However, there is no great control over the properties of said paper, since the quality of the paper can vary from one manufacturer to another and it is known that nitrocellulose has a WCA of 21 ° to 67 °, this level of hydrophilicity can induce a higher probability of a false positive signal. For that reason, paper analytical platforms generally suffer from insufficient sensitivity. Therefore, it is necessary to develop a paper with an adapted surface morphology, surface properties and adjusted wettability for specific purposes.
Por ejemplo, el documento W02007/106050 describe una prueba ELISA para detectar anticuerpos específicos de miembros de los flavivirus a partir de muestras biológicas, y se basa en una competencia por la unión del epítopo en la proteína de la envoltura del antígeno de flavivirus capturado usando IgA anti-flavivirus en presencia de suero positivo de flavivirus. Por su parte, el documento CN205679623U describe una fibra de nitrocelulosa cubierta de oro coloidal y kit de detección del antígeno del virus del dengue a partir de muestras de sangre. For example, W02007 / 106050 describes an ELISA test to detect flavivirus member-specific antibodies from biological samples, and is based on a competition for epitope binding on the envelope protein of captured flavivirus antigen using Anti-flavivirus IgA in the presence of positive flavivirus serum. For its part, document CN205679623U describes a nitrocellulose fiber covered with colloidal gold and a dengue virus antigen detection kit from blood samples.
Sin embargo, ninguno de los documentos sugiere el uso de una sonda que permita suspender una fibra de manera vertical sin utilizar adhesivos para duplicar el área de contacto en el ensayo. Tampoco anticipan el uso de un arreglo específico de reacción de polimerización por radicales libres, electrospinning y finalmente una impresión 3D o moldeo por inyección para obtener una alta sensibilidad de detección, estabilidad y vida útil del material. However, none of the documents suggest the use of a probe that allows a fiber to be suspended vertically without using adhesives to double the contact area in the test. They also do not anticipate the use of a specific reaction arrangement of free radical polymerization, electrospinning and finally a 3D printing or injection molding to obtain high detection sensitivity, stability and useful life of the material.
Por consecuencia de lo anterior, se ha buscado suprimir los inconvenientes que presentan los ensayos biológicos a base de papel o fibras utilizados en la actualidad, desarrollando un kit y dispositivo cilindrico para la detección de un agente infeccioso que, además de ser estable y realizar un diagnóstico con una alta sensibilidad, permita aumentar el área de contacto de la fibra utilizada, sin utilizar algún adhesivo y reduciendo los costos de operación.
OBJETOS DE LA INVENCIÓN As a consequence of the foregoing, it has been sought to eliminate the drawbacks presented by the biological tests based on paper or fibers currently used, developing a kit and cylindrical device for the detection of an infectious agent that, in addition to being stable and performing a Diagnostic with a high sensitivity, allows to increase the contact area of the fiber used, without using any adhesive and reducing operating costs. OBJECTS OF THE INVENTION
Teniendo en cuenta los defectos de la técnica anterior, es un objeto de la presente invención proporcionar un kit para la detección de un agente infeccioso sin utilizar adhesivos con una alta sensibilidad. Taking into account the shortcomings of the prior art, it is an object of the present invention to provide a kit for the detection of an infectious agent without using adhesives with a high sensitivity.
Otro objeto de la presente invención es proporcionar un kit para la detección de un agente infeccioso que utiliza un dispositivo cilindrico para brindar una mayor área de contacto y así reducir los costos de operación. Another object of the present invention is to provide a kit for the detection of an infectious agent that uses a cylindrical device to provide a greater contact area and thus reduce operating costs.
Estos y otros objetos se logran mediante unkit y dispositivo cilindrico para la detección de un agente infeccioso de conformidad con la presente invención. These and other objects are achieved by a kit and cylindrical device for the detection of an infectious agent in accordance with the present invention.
BREVE DESCRIPCIÓN DE LA INVENCIÓN. BRIEF DESCRIPTION OF THE INVENTION.
Se ha inventado un kit para la detección de un agente infeccioso con un dispositivo cilindrico, tipo sonda, que permite sumergir una fibra de detección de forma vertical, sin utilizar adhesivos, para duplicar su área de contacto y de esta forma obtener un diagnóstico con alta sensibilidad y menor costo de operación. A kit has been invented for the detection of an infectious agent with a cylindrical device, probe type, which allows a detection fiber to be submerged vertically, without using adhesives, to double its contact area and thus obtain a high diagnosis. sensitivity and lower operating cost.
De esta forma, un primer aspecto de la presente invención es un dispositivo para la detección de un agente infeccioso caracterizado porque comprende; i) una pieza hembra que a su vez comprende al menos una hendidura; y ii) una pieza macho que a su vez comprende al menos una prolongación para que las caras externas de cada prolongación se ajusten a cada una de las hendiduras de la pieza hembra, y de esta forma las dos piezas puedan unirse permitiendo la sujeción de una fibra de detección de forma vertical por uno de sus extremos, en donde el dispositivo tiene una forma tal que permite su acoplamiento con los pozos de una charola común utilizada en pruebas de detección de un agente infeccioso. Thus, a first aspect of the present invention is a device for the detection of an infectious agent characterized in that it comprises; i) a female part which in turn comprises at least one slit; and ii) a male piece that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female piece, and in this way the two pieces can be joined allowing the fastening of a detection fiber vertically at one of its ends, where the device is shaped such that it can be coupled with the wells of a common tray used in tests for the detection of an infectious agent.
Ahora bien, un segundo aspecto de la presente invención es un kit para la detección de un agente infeccioso caracterizado porque comprende: a) al menos una fibra de detección que a su vez comprende un anticuerpo predeterminado para la detección de un agente infeccioso predeterminado; b) al menos un dispositivo para la detección de un agente infeccioso caracterizado porque comprende: i) una pieza hembra que a su vez comprende al menos una hendidura; y ¡i) una pieza macho que a su vez comprende al menos una prolongación para que las caras externas de cada prolongación se ajusten a cada uno de las hendiduras de la pieza hembra, y de esta forma las dos piezas puedan unirse permitiendo la sujeción de la fibra de detección de forma vertical por uno de sus extremos; y c) una microplaca que a su vez comprende una pluralidad de pocilios y una solución dentro de cada pocilio que potencialmente comprende un biomarcador relacionado con un agente infeccioso predeterminado, en donde cada dispositivo se acopla con uno de los pocilios de la pluralidad de pocilios permitiendo que la fibra de detección se sumerja en la solución.
BREVE DESCRIPCIÓN DE LOS DIBUJOS Now, a second aspect of the present invention is a kit for the detection of an infectious agent characterized in that it comprises: a) at least one detection fiber which in turn comprises a predetermined antibody for the detection of a predetermined infectious agent; b) at least one device for detecting an infectious agent characterized in that it comprises: i) a female part which in turn comprises at least one slit; and i) a male part that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female part, and in this way the two parts can be joined allowing the fastening of the detection fiber vertically at one of its ends; and c) a microplate that in turn comprises a plurality of wells and a solution within each well that potentially comprises a biomarker related to a predetermined infectious agent, wherein each device is coupled with one of the wells of the plurality of wells allowing the detection fiber is immersed in the solution. BRIEF DESCRIPTION OF THE DRAWINGS
Los aspectos novedosos que se consideran característicos de la presente invención, se establecerán con particularidad en las reivindicaciones anexas. Sin embargo, algunas modalidades, características y algunos objetos y ventajas de la misma, se comprenderán mejor en la descripción detallada, cuando se lea en relación con los dibujos anexos, en los cuales: The novel aspects that are considered characteristic of the present invention will be set forth with particularity in the appended claims. However, some modalities, characteristics and some objects and advantages thereof, will be better understood in the detailed description, when read in connection with the attached drawings, in which:
La figura 1 muestra una vista en perspectiva de una modalidad de un dispositivo para la detección de un agente infeccioso (1100) de conformidad con los principios de la presente invención. Figure 1 shows a perspective view of an embodiment of an infectious agent detection device (1100) in accordance with the principles of the present invention.
La figura 2 muestra una vista en perspectiva de una modalidad de ensamblado del dispositivo para la detección de un agente infeccioso (1100) mostrado en la figura 1. Figure 2 shows a perspective view of one embodiment of the device for detecting an infectious agent (1100) shown in Figure 1.
La figura 3 muestra una vista lateral de una modalidad de un kit para la detección de un agente infeccioso (1000) de conformidad con los principios de la presente invención. Figure 3 shows a side view of an embodiment of a kit for the detection of an infectious agent (1000) in accordance with the principles of the present invention.
La figura 4 muestra una fotografía en perspectiva de una modalidad de un kit para la detección de un agente infeccioso (1000) de conformidad con los principios de la presente invención. Figure 4 shows a perspective photograph of an embodiment of a kit for the detection of an infectious agent (1000) in accordance with the principles of the present invention.
DESCRIPCIÓN DETALLADA DE LA INVENCIÓN DETAILED DESCRIPTION OF THE INVENTION
Un primer aspecto de la presente invención es un dispositivo para la detección de un agente infeccioso caracterizado porque comprende: i) una pieza hembra que a su vez comprende al menos una hendidura; y ii) una pieza macho que a su vez comprende al menos una prolongación para que las caras externas de cada prolongación se ajusten a cada una de las hendiduras de la pieza hembra, y de esta forma las dos piezas puedan unirse permitiendo la sujeción de una fibra de detección de forma vertical por uno de sus extremos, en donde el dispositivo tiene una forma tal que permite su acoplamiento con los pozos de una charola común utilizada en pruebas de detección de un agente infeccioso. A first aspect of the present invention is a device for the detection of an infectious agent characterized in that it comprises: i) a female part which in turn comprises at least one slit; and ii) a male piece that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female piece, and in this way the two pieces can be joined allowing the fastening of a detection fiber vertically at one of its ends, where the device is shaped such that it can be coupled with the wells of a common tray used in tests for the detection of an infectious agent.
En una modalidad preferida de la presente invención, el agente infeccioso a detectar está relacionado con una enfermedad tropical, más preferiblemente con esquistosomiasis, oncocercosis, filariasis linfática, leishmaniasis, lepra, dengue y dengue grave, pian, equinococosis, teniasis y cisticercosis, rabia, la enfermedad de Chagas, úlcera de Buruli, la tripanosomiasis africana humana, enfermedades transmitidas por vectores, dracunculosis. In a preferred embodiment of the present invention, the infectious agent to be detected is related to a tropical disease, more preferably to schistosomiasis, onchocerciasis, lymphatic filariasis, leishmaniasis, leprosy, dengue and severe dengue, yaws, echinococcosis, taeniasis and cysticercosis, rabies, Chagas disease, Buruli ulcer, human African trypanosomiasis, vector-borne diseases, dracunculiasis.
En una modalidad preferida de la presente invención, la pieza hembra tiene una forma de medio cilindro. In a preferred embodiment of the present invention, the female part has a half-cylinder shape.
Asimismo, dicha pieza hembra comprende preferiblemente dos hendiduras. Likewise, said female part preferably comprises two slits.
En una modalidad preferida de la presente invención, la pieza macho tiene una forma de medio cilindro. In a preferred embodiment of the present invention, the male part has a half-cylinder shape.
Asimismo, dicha pieza macho comprende preferiblemente dos prolongaciones. Likewise, said male part preferably comprises two extensions.
En relación con dichas pieza hembra y macho, preferiblemente ambas se obtienen mediante impresión 3D o moldeo por inyección. In relation to said female and male parts, preferably both are obtained by 3D printing or injection molding.
Al momento de unir ambas piezas para formar un dispositivo con forma cilindrica, dicho dispositivo cilindrico preferiblemente tiene un diámetro de 5 mm.
En una modalidad de la presente invención, la fibra de detección se selecciona de fibras de polímero electrohiladas. When joining both pieces to form a cylindrical shaped device, said cylindrical device preferably has a diameter of 5 mm. In one embodiment of the present invention, the detection fiber is selected from electrospun polymer fibers.
Un segundo aspecto de la presente invención es un kit para la detección de un agente infeccioso caracterizado porque comprende; a) al menos una fibra de detección que a su vez comprende un anticuerpo predeterminado para la detección de un agente infeccioso predeterminado; b) al menos un dispositivo para la detección de un agente infeccioso caracterizado porque comprende: i) una pieza hembra que a su vez comprende al menos una hendidura; y ¡i) una pieza macho que a su vez comprende al menos una prolongación para que las caras externas de cada prolongación se ajusten a cada uno de las hendiduras de la pieza hembra, y de esta forma las dos piezas puedan unirse permitiendo la sujeción de la fibra de detección de forma vertical por uno de sus extremos; y c) una microplaca que a su vez comprende una pluralidad de pocilios y una solución dentro de cada pocilio que potencialmente comprende un biomarcador relacionado con un agente infeccioso predeterminado, en donde cada dispositivo se acopla con uno de los pocilios de la pluralidad de pocilios permitiendo que la fibra de detección se sumerja en la solución. A second aspect of the present invention is a kit for the detection of an infectious agent characterized in that it comprises; a) at least one detection fiber which in turn comprises a predetermined antibody for the detection of a predetermined infectious agent; b) at least one device for detecting an infectious agent characterized in that it comprises: i) a female part which in turn comprises at least one slit; and i) a male part that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female part, and in this way the two parts can be joined allowing the fastening of the detection fiber vertically at one of its ends; and c) a microplate that in turn comprises a plurality of wells and a solution within each well that potentially comprises a biomarker related to a predetermined infectious agent, wherein each device is coupled with one of the wells of the plurality of wells allowing the detection fiber is immersed in the solution.
El kit propuesto representa una herramienta en el diagnóstico temprano de un agente infeccioso que tiene una operación similar a la de un ensayo inmunoabsorbente ligado a enzimas (ELISA) convencional, por lo que es conveniente para el técnico de laboratorio que realiza ELISA de forma regular. The proposed kit represents a tool in the early diagnosis of an infectious agent that has an operation similar to that of a conventional enzyme-linked immunosorbent assay (ELISA), making it convenient for the laboratory technician who performs ELISA on a regular basis.
En una modalidad preferida de la presente invención, el agente infeccioso a detectar es una enfermedad tropical, más preferiblemente se selecciona de esquistosomiasis, oncocercosis, filariasis linfática, leishmaniasis, lepra, dengue y dengue grave, pian, equinococosis, teniasis y cisticercosis, rabia, la enfermedad de Chagas, úlcera de Buruli, la tripanosomiasis africana humana, enfermedades transmitidas por vectores, dracunculosis. Aún más preferiblemente el agente infeccioso es el virus del dengue, el cual de forma preferida se detecta a través de la proteína tipo NS1, características del virus del dengue y que sirve como biomarcador. In a preferred embodiment of the present invention, the infectious agent to be detected is a tropical disease, more preferably it is selected from schistosomiasis, onchocerciasis, lymphatic filariasis, leishmaniasis, leprosy, dengue and severe dengue, yaws, echinococcosis, taeniasis and cysticercosis, rabies, Chagas disease, Buruli ulcer, human African trypanosomiasis, vector-borne diseases, dracunculiasis. Even more preferably the infectious agent is the dengue virus, which is preferably detected through the NS1-type protein, characteristic of the dengue virus and which serves as a biomarker.
En una modalidad de la presente invención, la fibra de detección se selecciona de fibras de polímero electrohiladas. In one embodiment of the present invention, the detection fiber is selected from electrospun polymer fibers.
En una modalidad preferida de la presente invención, el anticuerpo predeterminado se selecciona de entre anticuerpos primarios y/o secundarios, y más específicamente el anticuerpo primario es la glucoproteína NS1 contra el virus del dengue y el anticuerpo secundario es la cadena pesada de cabra anti-ratón IgG2a. In a preferred embodiment of the present invention, the predetermined antibody is selected from primary and / or secondary antibodies, and more specifically the primary antibody is the NS1 glycoprotein against dengue virus and the secondary antibody is the goat anti-dengue heavy chain. mouse IgG2a.
En una modalidad preferida de la presente invención, la pieza hembra tiene una forma de medio cilindro. In a preferred embodiment of the present invention, the female part has a half-cylinder shape.
Asimismo, dicha pieza hembra comprende preferiblemente dos hendiduras,Likewise, said female part preferably comprises two slits,
En una modalidad preferida de la presente invención, la pieza macho tiene una forma de medio cilindro. In a preferred embodiment of the present invention, the male part has a half-cylinder shape.
Asimismo, dicha pieza macho comprende preferiblemente dos prolongaciones.
En relación con dichas pieza hembra y macho, preferiblemente ambas se obtienen mediante impresión 3D o moldeo por inyección. Likewise, said male part preferably comprises two extensions. In relation to said female and male parts, preferably both are obtained by 3D printing or injection molding.
En una modalidad preferida de la presente invención, la pluralidad de pocilios comprendeIn a preferred embodiment of the present invention, the plurality of wells comprise
96 pocilios. 96 wells.
Una de las ventajas de la presente invención, es que las fibra de detección se puede sumergir en posición vertical, lo que proporciona una interacción efectiva con ambos lados de dicha fibra, además la presente invención hace innecesaria la aplicación de cualquier tipo de pegamento que, a su vez, minimiza el riesgo de desactivación de proteínas cuando entra en contacto con materiales adhesivos. One of the advantages of the present invention is that the detection fibers can be submerged in a vertical position, which provides an effective interaction with both sides of said fiber, in addition, the present invention makes the application of any type of glue unnecessary, which, in turn, it minimizes the risk of protein deactivation when it comes into contact with adhesive materials.
Haciendo referencia ahora a la figura 1, en ésta se muestra una vista en perspectiva de una modalidad de un dispositivo para la detección de un agente infeccioso (1100) de conformidad con los principios de la presente invención. Como se puede observar en dicha figura, el dispositivo (1100) comprende una pieza hembra (1110) con dos hendiduras (lili) y una pieza macho (1120) con 2 prolongaciones (1121), además se muestra una fibra de detección (1200). Referring now to Figure 1, there is shown a perspective view of one embodiment of an infectious agent detection device 1100 in accordance with the principles of the present invention. As can be seen in said figure, the device (1100) comprises a female part (1110) with two slits (lili) and a male part (1120) with 2 extensions (1121), in addition a detection fiber (1200) is shown .
Haciendo referencia a la figura 2, en ésta se muestra una vista en perspectiva de una modalidad de ensamblado del dispositivo para la detección de un agente infeccioso (1100) mostrado en la figura 1. Como se puede observar en dicha figura, las dos prolongaciones (1121) de la pieza macho (1120) se ajustan con las dos hendiduras (lili) de la pieza hembra (1110), de tal forma que sujetan la fibra de detección (1200) Referring to Figure 2, it shows a perspective view of an assembly modality of the device for the detection of an infectious agent (1100) shown in Figure 1. As can be seen in said figure, the two extensions ( 1121) of the male part (1120) fit with the two slits (lili) of the female part (1110), in such a way that they hold the detection fiber (1200)
Haciendo referencia a la figura 3, ésta muestra una vista lateral de una modalidad de un kit para la detección de un agente infeccioso (1000) de conformidad con los principios de la presente invención. Como se puede observar en dicha figura, el kit (1000) comprende una pluralidad de dispositivos para la detección de un agente infeccioso (1100); una microplaca (1300) con una pluralidad de pocilios (1310); y una pluralidad de fibras de detección (1200), en donde cada uno de los dispositivos (1100) se encuentran acoplados en cada uno de los pocilios (1310, de tal forma que la fibra de detección (1200) se encuentra sumergida de forma vertical en una solución (1311) que contiene potencialmente un agente infeccioso. Referring to Figure 3, this shows a side view of an embodiment of a kit for the detection of an infectious agent (1000) in accordance with the principles of the present invention. As can be seen in said figure, the kit (1000) comprises a plurality of devices for the detection of an infectious agent (1100); a microplate (1300) with a plurality of wells (1310); and a plurality of detection fibers (1200), where each of the devices (1100) are coupled in each of the wells (1310, in such a way that the detection fiber (1200) is submerged vertically in a solution (1311) potentially containing an infectious agent.
Haciendo referencia a la figura 4, ésta muestra una fotografía en perspectiva de una modalidad de un kit para la detección de un agente infeccioso (1000) de conformidad con los principios de la presente invención. Como se puede observar en dicha figura, el kit (1000) comprende un dispositivo (1100) con una fibra de detección (1200) sumergida vertical mente. 1 Referring to Figure 4, this shows a perspective photograph of an embodiment of a kit for the detection of an infectious agent (1000) in accordance with the principles of the present invention. As can be seen in said figure, the kit (1000) comprises a device (1100) with a detection fiber (1200) submerged vertically. 1
La presente invención será mejor entendida a partir de los siguientes ejemplos, los cuales se presentan únicamente con fines ilustrativos para permitir la comprensión cabal de las modalidades preferidas de la presente invención, sin que por ello se implique que no existen otras modalidades no ilustradas que puedan llevarse a la práctica con base en la descripción detallada arriba realizada. The present invention will be better understood from the following examples, which are presented for illustrative purposes only to allow a thorough understanding of the preferred embodiments of the present invention, without implying that there are no other, non-illustrated embodiments that may be put into practice based on the detailed description made above.
Ejemplo 1. Example 1.
Se realizó un ensayo para fabricar dispositivos cilindricos de conformidad con la presente invención.
Para ello, las piezas hembra y macho del dispositivo cilindrico se fabricaron utilizando un sistema Fortus® 400 me FDM de plástico ABS-M30 con una dimensión total de 5 mm, de tal forma que se pueda introducir a uno de la pluralidad de pocilios de una microplaca. El esquema de impresión se diseñó con SolidWorks® y se imprimieron cien pares de piezas para el desarrollo de cada microplaca de 96 pocilios. A trial was carried out to make cylindrical devices in accordance with the present invention. For this, the female and male parts of the cylindrical device were manufactured using a Fortus® 400 me FDM system in ABS-M30 plastic with a total dimension of 5 mm, so that it can be inserted into one of the plurality of wells of a microplate. The print scheme was designed with SolidWorks® and one hundred pairs of parts were printed for the development of each 96-well microplate.
Una vez fabricados los cien pares, se colocó una fibra de detección, obtenida mediante electrohilado, entre una pieza hembra y una pieza macho del dispositivo cilindrico, y se realizó lo anterior con los 95 pares restantes y así obtener 96 dispositivos cilindricos, en donde cada uno sostiene una fibra de detección. Once the hundred pairs had been manufactured, a detection fiber, obtained by electrospinning, was placed between a female part and a male part of the cylindrical device, and the above was done with the remaining 95 pairs and thus obtain 96 cylindrical devices, where each one holds a sense fiber.
Por último, se montó cada uno de los 96 dispositivos cilindricos en cada uno de los 96 pocilios de la tapa de una microplaca común. Finally, each of the 96 cylindrical devices was mounted in each of the 96 wells on the lid of a common microplate.
Ejemplo 2. Example 2.
Se realizó un ensayo para preparar soluciones para proteína NS1 del virus del dengue y soluciones para anticuerpos de conformidad con la presente invención, con los siguientes pasos: a. preparar una solución madre de tampón fosfato salino "PBS" (10X) añadiendo 161.2 g de NaCI, 4.4 g de KCI, 24 g de Na2HPC¡4 y 4 g de KH2PO4 a 2,000 mL de agua destilada. b. utilizar un tampón fosfato salino diluido (IX) como tampón de lavado, el cual se utiliza después de recubrir muestras con una solución de proteína NS1. c. preparar un tampón de recubrimiento, para la preparación de la solución de proteína NS1, con 100 mL de agua destilada, 2 mL de carbonato de sodio (feCCh) a 0.2 M y 23 mL de bicarbonato de sodio (NaHCC ) a 0.2 M; d. preparar un tampón de bloqueo para reducir la posibilidad de unión no específica, con albúmina de suero bovino "BSA" al 3 % en tampón fosfato salino (IX); e. emplear polisorbato 20 "Tween 20®" al 0.05 % en tampón fosfato salino (IX) como tampón de lavado después de recubrir las muestras con anticuerpos primarios de glucoproteína NS1 contra el virus del dengue (Anti-Dengue virus NS1 glycoprotein primary antibody) y el anticuerpo secundario es la cadena pesada de cabra anti-ratón IgG2a "HRP" (Goat anti-mouse IgG2a heavy chain. f. usar albúmina de suero bovino al 1 % en PBS (IX) como tampón de dilución para la preparación de las soluciones de anticuerpos. An assay was performed to prepare dengue virus NS1 protein solutions and antibody solutions according to the present invention, with the following steps: a. Prepare a stock solution of phosphate buffered saline "PBS" (10X) by adding 161.2 g of NaCl, 4.4 g of KCI, 24 g of Na2HPC¡4 and 4 g of KH2PO4 to 2,000 mL of distilled water. b. use dilute phosphate saline buffer (IX) as wash buffer, which is used after coating samples with NS1 protein solution. c. prepare a coating buffer, for the preparation of the NS1 protein solution, with 100 mL of distilled water, 2 mL of 0.2 M sodium carbonate (feCCh) and 23 mL of 0.2 M sodium bicarbonate (NaHCC); d. preparing a blocking buffer to reduce the possibility of non-specific binding, with 3% bovine serum albumin "BSA" in phosphate buffered saline (IX); and. Use 0.05% polysorbate 20 "Tween 20®" in phosphate buffered saline (IX) as wash buffer after coating samples with NS1 glycoprotein primary antibodies against dengue virus (Anti-Dengue virus NS1 glycoprotein primary antibody) and Secondary antibody is goat anti-mouse IgG2a heavy chain "HRP" (Goat anti-mouse IgG2a heavy chain. f. use 1% bovine serum albumin in PBS (IX) as dilution buffer for the preparation of the solutions of antibodies.
Ejemplo 3. Example 3.
Se realizó un ensayo para realizar el diagnóstico de dengue a través de la detección del biomarcador NS1 de conformidad con la presente invención, con los siguientes pasos: a. Lavar 3 veces, cada 5 minutos, cada uno de la pluralidad de pocilios de una charola con 200 pl por pocilio de tampón fosfato salino (IX) a temperatura ambiente, usando un agitador con una velocidad de agitación de 1000 rpm; b. incubar soluciones de NS1 a concentraciones de: 5, 50, 5xl02, 5xl03, 5xl04 y 5x10s Pg/mL a 4°C durante 8 horas, para cada concentración se realizaron 12 réplicas positivas y 8 negativas, las réplicas negativas se prepararon en ausencia de la proteína NS1;
c. cargar cada uno de la pluralidad de pocilios con 150 pl de las soluciones de NS1 del paso anterior; d. bloquear con la tapa, montada en el ejemplo 1, cada uno de la pluralidad de pocilios agregando 200 pL de tampón de bloqueo a cada pocilio. La incubación se llevó a cabo a 37 °C durante 1 hora; e. preparar una solución primaria de anticuerpo a 1:10,000 en tampón de dilución, cada pocilio recibió 150 pL de solución primaria y se bloqueó e incubó durante 2 horas a 37 °C); f. lavar 3 veces, cada 5 minutos, con 200 pl por pocilio de solución Tween-20 (ejemplo anterior) a temperatura ambiente, usando un agitador con una velocidad de agitación de 1000 rpm; g. adicionar 150 pL de solución secundaria de anticuerpo a 1:10,000 en tampón de dilución y bloquear e incubar durante 1 hora a 37 °C; h. lavar 3 veces, cada 5 minutos, con 200 pl por pocilio de solución Tween-20 (ejemplo anterior) a temperatura ambiente, usando un agitador con una velocidad de agitación de 1000 rpm; i. cargar cada uno de la pluralidad de pocilios con 100 pl de sustrato de ELISA Tetrametilbencidina "TMB" y bloquear e incubar durante 10 minutos; j. registre la lectura de absorbancia a 370 nm según las instrucciones del fabricante. An assay was performed to make the diagnosis of dengue through the detection of the biomarker NS1 in accordance with the present invention, with the following steps: a. Wash 3 times, every 5 minutes, each of the plurality of wells of a tray with 200 µl per well of phosphate buffer saline (IX) at room temperature, using a shaker with a stirring speed of 1000 rpm; b. incubate solutions of NS1 at concentrations of: 5, 50, 5xl0 2 , 5xl0 3 , 5xl0 4 and 5x10 s Pg / mL at 4 ° C for 8 hours, for each concentration 12 positive and 8 negative replicates were made, the negative replicates were prepared in the absence of the NS1 protein; c. loading each of the plurality of wells with 150 µl of the NS1 solutions from the previous step; d. Block with the lid, assembled in Example 1, each of the plurality of wells by adding 200 pL of blocking buffer to each well. Incubation was carried out at 37 ° C for 1 hour; and. prepare a 1: 10,000 stock antibody solution in dilution buffer, each well received 150 pL of stock solution and was blocked and incubated for 2 hours at 37 ° C); F. wash 3 times, every 5 minutes, with 200 µl per well of Tween-20 solution (previous example) at room temperature, using a shaker with a stirring speed of 1000 rpm; g. add 150 pL of secondary antibody solution at 1: 10,000 in dilution buffer and block and incubate for 1 hour at 37 ° C; h. wash 3 times, every 5 minutes, with 200 µl per well of Tween-20 solution (previous example) at room temperature, using a shaker with a stirring speed of 1000 rpm; i. load each of the plurality of wells with 100 µl of Tetramethylbenzidine ELISA substrate "TMB" and block and incubate for 10 minutes; j. Record the absorbance reading at 370 nm according to the manufacturer's instructions.
De conformidad con lo anteriormente descrito, se podrá observar que el kit y dispositivo cilindrico para la detección de un agente infeccioso, ha sido ideado para el diagnóstico temprano de una enfermedad tropical, y será evidente para cualquier experto en la materia que las modalidades de kit y dispositivo cilindrico para la detección de un agente infeccioso según se describió anteriormente e ilustró en los dibujos que se acompañan, son únicamente ilustrativas más no limitativas de la presente invención, ya que son posibles numerosos cambios de consideración en sus detalles sin apartarse del alcance de la invención. Por lo tanto, la presente invención no deberá considerarse como restringida excepto por lo que exija la técnica anterior y por el alcance de las reivindicaciones anexas.
In accordance with the foregoing, it can be seen that the kit and cylindrical device for the detection of an infectious agent has been designed for the early diagnosis of a tropical disease, and it will be evident to any expert in the field that the kit modalities and cylindrical device for the detection of an infectious agent as described above and illustrated in the accompanying drawings, are only illustrative but not limiting of the present invention, since numerous changes of consideration in its details are possible without departing from the scope of the invention. Therefore, the present invention should not be construed as restricted except as required by the prior art and for the scope of the appended claims.
Claims
1. Un dispositivo para la detección de un agente infeccioso caracterizado porque comprende: i) una pieza hembra que a su vez comprende al menos una hendidura; y ii) una pieza macho que a su vez comprende al menos una prolongación para que las caras externas de cada prolongación se ajusten a cada una de las hendiduras de la pieza hembra, y de esta forma las dos piezas puedan unirse permitiendo la sujeción de una fibra de detección de forma vertical por uno de sus extremos, en donde el dispositivo tiene una forma tal que permite su acoplamiento con los pozos de una charola común utilizada en pruebas de detección de un agente infeccioso. 1. A device for detecting an infectious agent characterized in that it comprises: i) a female part which in turn comprises at least one slit; and ii) a male piece that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female piece, and in this way the two pieces can be joined allowing the fastening of a detection fiber vertically at one of its ends, where the device is shaped such that it can be coupled with the wells of a common tray used in tests for the detection of an infectious agent.
2. El dispositivo de conformidad con la reivindicación 1, caracterizado además porque el agente infeccioso está relacionado con una enfermedad tropical. 2. The device according to claim 1, further characterized in that the infectious agent is related to a tropical disease.
3. El dispositivo de conformidad con la reivindicación 2, caracterizado además porque la enfermedad tropical se selecciona de entre esquistosomiasis, oncocercosis, filariasis linfática, leishmaniasis, lepra, dengue y dengue grave, pian, equinococosis, teniasis y cisticercosis, rabia, la enfermedad de Chagas, úlcera de Buruli, la tripanosomiasis africana humana, enfermedades transmitidas por vectores, o dracunculosis. 3. The device according to claim 2, further characterized in that the tropical disease is selected from schistosomiasis, onchocerciasis, lymphatic filariasis, leishmaniasis, leprosy, dengue and severe dengue, yaws, echinococcosis, taeniasis and cysticercosis, rabies, the disease of Chagas disease, Buruli ulcer, human African trypanosomiasis, vector-borne diseases, or guinea worm.
4. El dispositivo de conformidad con la reivindicación 1, caracterizado además porque la pieza hembra tiene una forma de medio cilindro. 4. The device according to claim 1, further characterized in that the female part has a half-cylinder shape.
5. El dispositivo de conformidad con la reivindicación 1, caracterizado además porque la pieza hembra comprende dos hendiduras. 5. The device according to claim 1, further characterized in that the female part comprises two slits.
6. El dispositivo de conformidad con la reivindicación 1, caracterizado además porque la pieza macho tiene una forma de medio cilindro. 6. The device according to claim 1, further characterized in that the male part has a half-cylinder shape.
7. El dispositivo de conformidad con la reivindicación 1, caracterizado además porque la pieza macho comprende dos prolongaciones. 7. The device according to claim 1, further characterized in that the male part comprises two extensions.
8. El dispositivo de conformidad con la reivindicación 1, caracterizado además porque las piezas hembra y macho se obtienen mediante impresión 3D o moldeo por inyección. The device according to claim 1, further characterized in that the female and male parts are obtained by 3D printing or injection molding.
9. El dispositivo de conformidad con la reivindicación 1, caracterizado además porque al momento de unir ambas piezas para formar un dispositivo de forma cilindrica. 9. The device according to claim 1, further characterized in that at the time of joining both pieces to form a cylindrical device.
10. El dispositivo de conformidad con la reivindicación 1, caracterizado además porque la fibra de detección se selecciona de fibras de polímero electrohiladas. The device according to claim 1, further characterized in that the detection fiber is selected from electrospun polymer fibers.
11. Un kit para la detección de un agente infeccioso caracterizado porque comprende: a) al menos una fibra de detección que a su vez comprende un anticuerpo predeterminado para la detección de un agente infeccioso; b) al menos un dispositivo para la detección de un agente infeccioso que comprende: i) una pieza hembra que a su vez comprende al menos una hendidura; y ii) una pieza macho que a su vez comprende al menos una prolongación para que las caras externas de cada prolongación se ajusten a cada una de las hendiduras de la pieza hembra, y de esta forma las dos piezas puedan unirse permitiendo la sujeción de la fibra de detección de forma vertical por uno de sus extremos; y c) una microplaca que a su vez comprende una pluralidad de pocilios y una solución dentro de cada pocilio que
potencialmente comprende un biomarcador relacionado con un agente infeccioso predeterminado, en donde cada dispositivo se acopla con uno de los pocilios de la pluralidad de pocilios permitiendo que la fibra de detección se sumerja en la solución. 11. A kit for the detection of an infectious agent characterized in that it comprises: a) at least one detection fiber which in turn comprises a predetermined antibody for the detection of an infectious agent; b) at least one device for detecting an infectious agent comprising: i) a female part which in turn comprises at least one slit; and ii) a male part that in turn comprises at least one extension so that the external faces of each extension fit into each of the grooves of the female part, and in this way the two parts can be joined allowing the fastening of the detection fiber vertically at one end; and c) a microplate that in turn comprises a plurality of wells and a solution within each well that potentially comprises a biomarker related to a predetermined infectious agent, where each device is coupled to one of the plurality of wells allowing the detection fiber to be immersed in the solution.
12. El kit de conformidad con la reivindicación 11, caracterizado además porque el agente infeccioso está relacionado con una enfermedad tropical. 12. The kit according to claim 11, further characterized in that the infectious agent is related to a tropical disease.
13. El kit de conformidad con la reivindicación 12, caracterizado además porque la enfermedad tropical se selecciona de entre esquistosomiasis, oncocercosis, filariasis linfática, leishmaniasis, lepra, dengue y dengue grave, pian, equinococosis, teniasis y cisticercosis, rabia, la enfermedad de Chagas, úlcera de Buruli, la tripanosomiasis africana humana, enfermedades transmitidas por vectores, o dracunculosis. The kit according to claim 12, further characterized in that the tropical disease is selected from schistosomiasis, onchocerciasis, lymphatic filariasis, leishmaniasis, leprosy, dengue and severe dengue, yaws, echinococcosis, taeniasis and cysticercosis, rabies, the disease of Chagas disease, Buruli ulcer, human African trypanosomiasis, vector-borne diseases, or guinea worm.
14. El kit de conformidad con la reivindicación 13, caracterizado además porque la enfermedad tropical es dengue. 14. The kit according to claim 13, further characterized in that the tropical disease is dengue.
15. El kit de conformidad con la reivindicación 11, caracterizado además porque la fibra de detección se selecciona de fibras de polímero electrohiladas. The kit according to claim 11, further characterized in that the detection fiber is selected from electrospun polymer fibers.
16. El kit de conformidad con la reivindicación 11, caracterizado además porque el anticuerpo predeterminado se selecciona de entre anticuerpos primarios y/o secundarios. 16. The kit according to claim 11, further characterized in that the predetermined antibody is selected from primary and / or secondary antibodies.
17. El kit de conformidad con la reivindicación 16, caracterizado además porque el anticuerpo primario es la glucoproteína NS1 contra el virus del dengue y el anticuerpo secundario es la cadena pesada de cabra anti-ratón IgG2a. 17. The kit according to claim 16, further characterized in that the primary antibody is the glycoprotein NS1 against dengue virus and the secondary antibody is the goat anti-mouse IgG2a heavy chain.
18. El kit de conformidad con la reivindicación 11, caracterizado además porque la pieza hembra tiene una forma de medio cilindro. 18. The kit according to claim 11, further characterized in that the female part has a half-cylinder shape.
19. El kit de conformidad con la reivindicación 11, caracterizado además porque la pieza hembra comprende dos hendiduras. 19. The kit according to claim 11, further characterized in that the female part comprises two slits.
20. El kit de conformidad con la reivindicación 11, caracterizado además porque ia pieza macho tiene una forma de medio cilindro. 20. The kit according to claim 11, further characterized in that the male part has a half-cylinder shape.
21. El kit de conformidad con la reivindicación 11, caracterizado además porque la pieza macho comprende dos prolongaciones. 21. The kit according to claim 11, further characterized in that the male part comprises two extensions.
22. El kit de conformidad con la reivindicación 11, caracterizado además porque la pluralidad de pocilios comprende 96 pocilios.
22. The kit according to claim 11, further characterized in that the plurality of wells comprises 96 wells.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MXMX/A/2019/014639 | 2019-12-05 | ||
| MX2019014639A MX2019014639A (en) | 2019-12-05 | 2019-12-05 | Kit and cylindrical device for detecting an infectious agent. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| WO2021112660A1 true WO2021112660A1 (en) | 2021-06-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PCT/MX2020/000047 WO2021112660A1 (en) | 2019-12-05 | 2020-11-23 | Kit and cylindrical device for the detection of an infectious agent |
Country Status (2)
| Country | Link |
|---|---|
| MX (1) | MX2019014639A (en) |
| WO (1) | WO2021112660A1 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080268466A1 (en) * | 2001-07-03 | 2008-10-30 | Xenotope Diagnostics, Inc. | Method and Device for Trichomonas Detection |
| KR101157042B1 (en) * | 2005-04-18 | 2012-06-21 | 주식회사 엘지생명과학 | Improved Lateral Flow Immunoassay And Device Therefor |
| KR101519897B1 (en) * | 2013-11-15 | 2015-05-14 | 바디텍메드 주식회사 | Cartridge with capillary structure for lateral flow analysis |
-
2019
- 2019-12-05 MX MX2019014639A patent/MX2019014639A/en unknown
-
2020
- 2020-11-23 WO PCT/MX2020/000047 patent/WO2021112660A1/en active Application Filing
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20080268466A1 (en) * | 2001-07-03 | 2008-10-30 | Xenotope Diagnostics, Inc. | Method and Device for Trichomonas Detection |
| KR101157042B1 (en) * | 2005-04-18 | 2012-06-21 | 주식회사 엘지생명과학 | Improved Lateral Flow Immunoassay And Device Therefor |
| KR101519897B1 (en) * | 2013-11-15 | 2015-05-14 | 바디텍메드 주식회사 | Cartridge with capillary structure for lateral flow analysis |
Also Published As
| Publication number | Publication date |
|---|---|
| MX2019014639A (en) | 2021-06-07 |
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