WO2019082640A1 - Electrophoretic support and electrophoresis device - Google Patents

Electrophoretic support and electrophoresis device

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Publication number
WO2019082640A1
WO2019082640A1 PCT/JP2018/037589 JP2018037589W WO2019082640A1 WO 2019082640 A1 WO2019082640 A1 WO 2019082640A1 JP 2018037589 W JP2018037589 W JP 2018037589W WO 2019082640 A1 WO2019082640 A1 WO 2019082640A1
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WO
WIPO (PCT)
Prior art keywords
metal oxide
oxide film
region
carrier
film pieces
Prior art date
Application number
PCT/JP2018/037589
Other languages
French (fr)
Japanese (ja)
Inventor
年伸 松野
俊裕 坂本
Original Assignee
パナソニックＩｐマネジメント株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by パナソニックＩｐマネジメント株式会社 filed Critical パナソニックＩｐマネジメント株式会社
Publication of WO2019082640A1 publication Critical patent/WO2019082640A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Definitions

  • the present invention relates to an electrophoresis support and an electrophoresis apparatus provided with the same.
  • Isoelectric focusing electrophoresis is known in which a sample is separated by utilizing the difference in the isoelectric point of the sample.
  • an electrophoresis apparatus is used (see, for example, Patent Document 1).
  • the electrophoresis apparatus includes a container, a pair of electrodes disposed inside the container, and an electrophoresis support disposed between the pair of electrodes.
  • the electrophoresis support has a porous carrier and a plurality of metal oxide films arranged in a line on the top surface of the carrier.
  • Each of the plurality of metal oxide films is formed of a plurality of metal oxides of different types.
  • the plurality of metal oxide films form a pH gradient on the carrier.
  • the conventional electrophoretic support described above requires one type of metal oxide to form one pH gradation. Therefore, in the case of forming a pH gradient having seven pH gradations, for example, pH 4 to pH 10, seven types of metal oxides equal in number to the pH gradation are required. As a result, as the pH gradation is increased, the number of steps for forming the plurality of metal oxide films increases, and the control of forming the plurality of metal oxide films becomes complicated. As a result, there arises a problem that the manufacturing process of the electrophoresis support becomes complicated.
  • the present invention provides an electrophoresis support capable of suppressing the manufacturing process from becoming complicated even when the number of pH gradations in the pH gradient is increased, and an electrophoresis apparatus including the same. Do.
  • An electrophoresis support is an electrophoresis support used in an electrophoresis apparatus for electrophoresis of a sample, which is a porous carrier, and includes a first region A carrier having a surface formed thereon, and a second region disposed adjacent to the first region, and a first for forming a first pH corresponding to a first isoelectric point of the sample A first metal oxide film disposed in the first region and formed of a first metal oxide, and a second metal oxide film corresponding to a second isoelectric point of the sample A second metal oxide film for forming the pH of the second metal oxide film, the second metal oxide film disposed in the second region and formed of the first metal oxide, and the carrier And the first area ratio occupied by the first metal oxide film in the first region is the second region. Different from the second area ratio occupied the second metal oxide film is in the.
  • the manufacturing process can be prevented from becoming complicated even when the number of pH gradations is increased.
  • FIG. 1 is a top view showing the configuration of the electrophoresis apparatus according to the first embodiment.
  • FIG. 2 is a cross-sectional view of the electrophoresis device according to the first embodiment, taken along line II-II in FIG.
  • FIG. 3 is a top view showing the structure of the metal oxide film of the electrophoresis support according to the first embodiment.
  • FIG. 4 is a top view showing the configuration of the metal oxide film of the electrophoresis support according to the second embodiment.
  • FIG. 5 is a top view showing the configuration of the metal oxide film of the electrophoresis support according to the third embodiment.
  • FIG. 6 is a top view showing the configuration of the metal oxide film of the electrophoresis support according to the fourth embodiment.
  • FIG. 1 is a top view showing the configuration of the electrophoresis apparatus according to the first embodiment.
  • FIG. 2 is a cross-sectional view of the electrophoresis device according to the first embodiment, taken along line II-II in FIG.
  • FIG. 7 is a cross-sectional view of an essential part of the electrophoresis support according to the fourth embodiment, taken along the line VII-VII in FIG.
  • FIG. 8 is a cross-sectional view of essential parts of the electrophoresis support according to the fourth embodiment, taken along line VIII-VIII in FIG.
  • FIG. 9 is a top view showing the configuration of the metal oxide film of the electrophoresis support according to the fifth embodiment.
  • FIG. 10 is a view for explaining an example of the film formation step of the metal oxide film of the electrophoresis support according to the fifth embodiment.
  • FIG. 11 is a graph for explaining the pH gradient formed by the metal oxide film of the electrophoresis support according to the fifth embodiment.
  • An electrophoresis support is an electrophoresis support used in an electrophoresis apparatus for electrophoresis of a sample, which is a porous carrier, and includes a first region A carrier having a surface formed thereon, and a second region disposed adjacent to the first region, and a first for forming a first pH corresponding to a first isoelectric point of the sample A first metal oxide film disposed in the first region and formed of a first metal oxide, and a second metal oxide film corresponding to a second isoelectric point of the sample A second metal oxide film for forming the pH of the second metal oxide film, the second metal oxide film disposed in the second region and formed of the first metal oxide, and the carrier And the first area ratio occupied by the first metal oxide film in the first region is the second region. Different from the second area ratio occupied the second metal oxide film is in the.
  • the first area ratio occupied by the first metal oxide film in the first region is the second area occupied by the second metal oxide film in the second region. It is different from the area ratio.
  • a third region is further formed opposite to the first region across the second region, and the electrophoresis support further includes: A third metal oxide film for forming a third pH corresponding to a third isoelectric point of a sample, wherein the third metal oxide film is disposed in the third region and formed of a second metal oxide You may comprise so that the metal oxide film of 3 may be provided.
  • the pH gradation of the pH gradient can be further subdivided.
  • a fourth region is further formed opposite to the second region across the third region, and the electrophoresis support further includes the fourth member.
  • It is a 4th metal oxide film for forming the 4th pH corresponding to the 4th isoelectric point of a sample, Comprising: It arrange
  • a fourth metal oxide film is provided, and the third area ratio occupied by the third metal oxide film in the third region when the carrier is viewed in plan is the fourth area ratio in the fourth region. It may be configured to be different from the fourth area ratio occupied by the metal oxide film.
  • the pH gradation of the pH gradient can be further subdivided.
  • the first isoelectric point is lower than the second isoelectric point and the second isoelectric point is lower than the isoelectric point corresponding to the pH of pure water
  • the first isoelectric point is lower than the second isoelectric point.
  • the area ratio of 2 may be configured to be smaller than the first area ratio.
  • the pH gradient can be formed such that the second pH formed by the second metal oxide film is higher than the first pH formed by the first metal oxide film.
  • the first isoelectric point is higher than the second isoelectric point, and the second isoelectric point is higher than the isoelectric point corresponding to the pH of pure water, the first isoelectric point is higher than the second isoelectric point.
  • the area ratio of 2 may be configured to be smaller than the first area ratio.
  • a fifth region is further formed on the surface of the carrier, the fifth region being disposed between the second region and the third region, and the electrophoresis support further comprises: A fifth metal oxide film for forming a fifth pH corresponding to a fifth isoelectric point, which is disposed in the fifth region, and the first metal oxide and the second metal You may comprise so that the 5th metal oxide film formed with the oxide may be provided.
  • the fifth metal oxide film formed of the first metal oxide and the second metal oxide is different from the second metal oxide film formed of the first metal oxide and the second metal oxide film.
  • the third embodiment is disposed between the second metal oxide film and the third metal oxide film formed of the second metal oxide, so that pH gradation of the pH gradient can be further subdivided.
  • the fifth metal oxide film is formed of the first metal oxide, and includes a plurality of first metal oxide film pieces scattered in the fifth region and the second metal oxide. And a plurality of second metal oxide film pieces formed and scattered in the fifth region, and when the carrier is viewed in plan, each of the plurality of first metal oxide film pieces is The plurality of second metal oxide film pieces may be arranged not to overlap with each other.
  • each of the plurality of first metal oxide film pieces is arranged so as not to overlap with each of the plurality of second metal oxide film pieces when the carrier is viewed in a plan view.
  • a contact area between each of the plurality of first metal oxide film pieces and the plurality of second metal oxide film pieces and a liquid such as pure water can be sufficiently secured.
  • each of the plurality of first metal oxide film pieces and the plurality of second metal oxide film pieces can efficiently generate the redox reaction to the liquid.
  • a sixth region is further formed, which is disposed between the third region and the fifth region, and the electrophoresis support further includes the sixth.
  • a sixth metal oxide film for forming a sixth pH corresponding to the sixth isoelectric point of the sample the sixth metal oxide film being the first metal
  • the sixth metal oxide film being the first metal
  • each of the plurality of third metal oxide film pieces does not overlap with each of the plurality of fourth metal oxide film pieces when the carrier is viewed in a plan view.
  • the plurality of first metal oxide film pieces and the plurality of third metal oxide film pieces are And differ by at least one of the number, and said a plurality of second metal oxide film pieces and the plurality of fourth metal oxide film pieces, at least one of the area and the number may be configured differently.
  • the pH gradation of the pH gradient can be further subdivided.
  • the fifth metal oxide film is formed of the first metal oxide, and includes a plurality of first metal oxide film pieces scattered in the fifth region and the second metal oxide. And a plurality of second metal oxide film pieces formed and scattered in the fifth region, and when the carrier is viewed in plan, each of the plurality of first metal oxide film pieces is It may be configured to be arranged to overlap with each of the plurality of second metal oxide film pieces.
  • each of the plurality of first metal oxide film pieces is disposed so as to overlap with each of the plurality of second metal oxide film pieces in plan view of the carrier. Even if the area of the region 5 can not be sufficiently secured, the fifth metal oxide film can be compactly disposed in the fifth region.
  • At least two section lengths among the section lengths of the first area, the second area, the third area, and the fourth area may be configured to be different from each other.
  • An electrophoresis apparatus is an electrophoresis apparatus for electrophoresis of a sample, comprising: a container; a pair of electrodes disposed inside the container; A migration support, and the pair of electrodes are respectively disposed at both ends of the electrophoresis support in the migration direction of the sample.
  • the manufacturing process of the electrophoresis support can be suppressed from being complicated.
  • Embodiment 1 [1-1. Configuration of electrophoresis apparatus] First, the configuration of the electrophoresis device 2 according to the first embodiment will be described with reference to FIGS. 1 and 2.
  • FIG. 1 is a top view showing the configuration of the electrophoresis device 2 according to the first embodiment.
  • FIG. 2 is a cross-sectional view of the electrophoresis device 2 according to the first embodiment, taken along line II-II in FIG.
  • the electrophoresis apparatus 2 is an apparatus for performing isoelectric focusing in which the sample 4 is separated using the difference in the isoelectric point of the sample 4.
  • the electrophoresis apparatus 2 includes a container 6, an electrophoresis support 8, a pair of electrodes 10 and 12, and a power supply device 14.
  • the sample 4 contains, for example, a plurality of proteins each having a plurality of different isoelectric points.
  • the container 6 has an opening 16 on the top surface.
  • the liquid 18 is stored inside the container 6.
  • the liquid 18 is, for example, pure water of pH 7.
  • the material of the container 6 is made of, for example, a resin such as a polymer, silicon, or a metal.
  • the electrophoresis support 8 has a carrier 20 and a metal oxide film 22.
  • the electrophoresis support 8 is disposed inside the container 6 and immersed in the liquid 18 inside the container 6.
  • the carrier 20 is formed of, for example, a porous body such as cellulose acetate.
  • the carrier 20 is formed in a long strip shape in the X-axis direction.
  • the size of the carrier 20 in the longitudinal direction (X-axis direction) is, for example, 30 mm, and the size of the carrier 20 in the lateral direction (Y-axis direction) is, for example, 2 mm.
  • the size of the carrier 20 in the thickness direction (Z-axis direction) is, for example, 0.15 mm.
  • the sample 4 migrates in the direction from the minus side to the plus side of the X axis along the longitudinal direction of the carrier 20.
  • the metal oxide film 22 is for forming a pH gradient along the longitudinal direction of the carrier 20.
  • the metal oxide film 22 is disposed on the upper surface 24 (an example of the surface) of the carrier 20.
  • the metal oxide film 22 forms a pH gradient having seven pH gradations, for example, pH 4 to pH 10, by the redox reaction to the liquid 18. The specific configuration of the metal oxide film 22 will be described later.
  • the pair of electrodes 10 and 12 are disposed inside the container 6 and immersed in the liquid 18 inside the container 6.
  • the pair of electrodes 10 and 12 function as an anode and a cathode, respectively, and are disposed at positions in contact with both ends in the longitudinal direction (the migration direction of the sample 4) of the carrier 20. That is, the electrophoresis support 8 is disposed between the pair of electrodes 10 and 12.
  • the material of each of the pair of electrodes 10 and 12 is formed of, for example, a conductive material such as gold, platinum, copper, carbon, or a composite thereof.
  • the pair of electrodes 10 and 12 are disposed between the electrophoresis support 8 and the side wall of the container 6, but the present invention is not limited to this.
  • a pair of electrodes 10 and 12 may be disposed between the electrophoresis support 8 and the bottom of the container 6.
  • the pair of electrodes 10 and 12 are disposed at positions contacting the both ends in the longitudinal direction of the carrier 20, respectively.
  • the power supply 14 is disposed outside the container 6 and electrically connected to each of the pair of electrodes 10 and 12.
  • the power supply device 14 applies a predetermined DC voltage between the pair of electrodes 10 and 12.
  • FIG. 3 is a top view showing the configuration of the metal oxide film 22 of the electrophoresis support 8 according to the first embodiment.
  • the metal oxide film body 22 has a plurality of metal oxide films 26 to 38.
  • the plurality of metal oxide films 26 to 38 are respectively disposed in the plurality of regions 40 to 52 on the upper surface 24 of the carrier 20.
  • the plurality of regions 40 to 52 are arranged in line in this order along the longitudinal direction of the carrier 20.
  • Each of the plurality of regions 40 to 52 is, for example, a rectangular region, and has the same section length (length in the X-axis direction).
  • the boundary lines of the plurality of regions 40 to 52 are represented by solid rectangular frames.
  • the metal oxide film 26 (an example of a first metal oxide film) is formed of TiO 2 (an example of a first metal oxide) which is a metal oxide.
  • the metal oxide film 26 is uniformly disposed over the entire region 40 (an example of the first region) of the carrier 20.
  • the metal oxide film 26 forms a pH gradation of, for example, pH 4.
  • the pH gradation of the liquid 18 in the vicinity of the region 40 is, for example, the isoelectric point of pH 4 of the sample 4 (first isoelectric point PH corresponding to 4) (an example of the first pH).
  • metal oxide film formed of TiO 2 which is an example of the metal oxide
  • hydrogen atoms (H) of OH groups present on the surface of the metal oxide film in pure water of pH 7 are released into the liquid.
  • the surface potential of the metal oxide film becomes negative.
  • the hydrogen atoms released into the solution become hydrogen ions (H + ) in the solution, so the solution in the vicinity of the metal oxide film becomes acidic (for example, pH 4).
  • acidic for example, pH 4
  • the metal oxide film 28 (an example of the second metal oxide film) is formed of TiO 2 similarly to the metal oxide film 26.
  • the metal oxide film 28 has a plurality of metal oxide film pieces 54 scattered in a lattice shape in the region 42 (an example of the second region) of the carrier 20. That is, the metal oxide film 28 is an aggregate of a plurality of metal oxide film pieces 54.
  • Each of the plurality of metal oxide film pieces 54 is formed in a circular shape in plan view. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 28 in the region 42 of the carrier 20 (an example of the second area ratio) is the area ratio occupied by the metal oxide film 26 in the region 40 of the carrier 20 (An example of the first area ratio) is smaller.
  • the metal oxide film 28 forms a pH gradation of, for example, pH 4.
  • the region 42 is formed by the exposed portion of the carrier 20 and the metal oxide film 28.
  • the metal oxide film 28 affects the pH gradation in the region 42. Therefore, the pH gradation of the region 42 is formed by a combination of the pH gradation (for example, pH 4) formed by the metal oxide film 28 and the pH gradation (for example, pH 7) formed by the pure water.
  • the pH gradation of the liquid 18 in the vicinity of the region 42 becomes, for example, pH 5 (an example of a second pH) corresponding to the isoelectric point (an example of a second isoelectric point) of pH 5 of the sample 4.
  • the metal oxide film 30 is formed of TiO 2 similarly to the metal oxide film 26.
  • the metal oxide film 30 has a plurality of metal oxide film pieces 56 interspersed in a lattice shape in the region 44 of the carrier 20. That is, the metal oxide film 30 is an aggregate of a plurality of metal oxide film pieces 56.
  • Each of the plurality of metal oxide film pieces 56 is formed in a circular shape in plan view.
  • the number of the plurality of metal oxide film pieces 56 is the same as the number of the plurality of metal oxide film pieces 54.
  • the diameter of each of the plurality of metal oxide film pieces 56 is smaller than the diameter of each of the plurality of metal oxide film pieces 54.
  • the area ratio occupied by the metal oxide film 30 in the region 44 of the carrier 20 is smaller than the area ratio occupied by the metal oxide film 28 in the region 42 of the carrier 20.
  • the metal oxide film 30 forms, for example, a pH gradation of pH4.
  • the region 44 is formed by the exposed portion of the carrier 20 and the metal oxide film 30.
  • the metal oxide film 30 affects the pH gradation in the region 44. Therefore, the pH gradation of the region 44 is formed by a combination of the pH gradation (for example, pH 4) formed by the metal oxide film 30 and the pH gradation (for example, pH 7) formed by the pure water.
  • the pH gradation of the liquid 18 in the vicinity of the region 44 becomes, for example, pH 6 corresponding to the isoelectric point of pH 6 of the sample 4.
  • the pH gradation formed by the region 44 is higher than the pH gradation formed by the region 42.
  • the metal oxide film 32 is formed of NiO which is a metal oxide.
  • the metal oxide film 32 is uniformly disposed over the entire region 46 of the carrier 20.
  • the metal oxide film 32 forms a pH gradation of, for example, pH 7.
  • the pH gradation of the liquid 18 in the vicinity of the region 46 becomes, for example, pH 7 corresponding to the isoelectric point of pH 7 of the sample 4.
  • OH groups present on the surface of the metal oxide film in pure water of pH 7 receive hydrogen ions (H + ) in the liquid. Thereby, the surface potential of the metal oxide film becomes positive. Since hydrogen ions (H + ) decrease in the solution, the solution in the vicinity of the metal oxide film becomes neutral (eg, pH 7).
  • the metal oxide film 34 is formed of MgO (an example of a second metal oxide) which is a metal oxide.
  • the metal oxide film 34 has a plurality of metal oxide film pieces 58 interspersed in a lattice shape in the region 48 of the carrier 20. That is, the metal oxide film 34 is an aggregate of a plurality of metal oxide film pieces 58. Each of the plurality of metal oxide film pieces 58 is formed in a circular shape in plan view.
  • the metal oxide film 34 forms a pH gradation of, for example, pH 10.
  • the region 48 is formed by the exposed portion of the carrier 20 and the metal oxide film 34.
  • the metal oxide film 34 affects the pH gradation in the region 48.
  • the pH gradation of the region 48 is formed by a combination of pH gradation (for example, pH 10) formed by the metal oxide film 34 and pH gradation (for example, pH 7) formed by pure water.
  • pH gradation for example, pH 10
  • pH gradation for example, pH 7
  • the pH gradation of the liquid 18 in the vicinity of the region 48 is, for example, pH 8 corresponding to the isoelectric point of pH 8 of the sample 4.
  • a metal oxide film formed of MgO which is an example of a metal oxide
  • OH groups present on the surface of the metal oxide film in pure water of pH 7 receive hydrogen ions (H + ) in the liquid.
  • the surface potential of the metal oxide film becomes positive.
  • hydrogen ions (H + ) decrease in the liquid, the solution in the vicinity of the metal oxide film becomes more alkaline (for example, pH 10).
  • alkaline for example, pH 10
  • the metal oxide film 36 (an example of the third metal oxide film) is formed of MgO, similarly to the metal oxide film 34.
  • the metal oxide film 36 has a plurality of metal oxide film pieces 60 interspersed in a lattice shape in a region 50 (an example of a third region) of the carrier 20. That is, the metal oxide film 36 is an aggregate of a plurality of metal oxide film pieces 60.
  • Each of the plurality of metal oxide film pieces 60 is formed in a circular shape in plan view.
  • the number of the plurality of metal oxide film pieces 60 is the same as the number of the plurality of metal oxide film pieces 58.
  • the diameter of each of the plurality of metal oxide film pieces 60 is larger than the diameter of each of the plurality of metal oxide film pieces 58.
  • the area ratio occupied by the metal oxide film 36 in the region 50 of the carrier 20 is larger than the area ratio occupied by the metal oxide film 34 in the region 48 of the carrier 20.
  • the metal oxide film 36 forms, for example, a pH gradation of pH 10.
  • Region 50 is formed of a portion where carrier 20 is exposed and metal oxide film 36.
  • the metal oxide film 36 affects the pH gradation in the region 50. Therefore, the pH gradation of the region 50 is formed by a combination of pH gradation (for example, pH 10) formed by the metal oxide film 36 and pH gradation (for example, pH 7) formed by pure water.
  • the pH gradation of the liquid 18 in the vicinity of the region 50 is, for example, pH 9 (an example of a third pH) corresponding to the isoelectric point (an example of a third isoelectric point) of pH 9 of the sample 4.
  • the surface area of MgO in contact with the liquid 18 decreases as the above area ratio decreases, so the OH groups present on the surface of MgO are hydrogen ions in the liquid 18 The amount of receiving (H + ) decreases. Therefore, the pH gradation formed by the region 50 is higher than the pH gradation formed by the region 48.
  • the metal oxide film 38 (an example of the fourth metal oxide film) is formed of MgO, similarly to the metal oxide film 34.
  • the metal oxide film 38 is uniformly disposed over the entire region 52 (an example of a fourth region) of the carrier 20. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 38 in the region 52 of the carrier 20 (an example of the fourth area ratio) is the area ratio occupied by the metal oxide film 36 in the region 50 of the carrier 20 (An example of the third area ratio) is larger.
  • the metal oxide film 38 forms, for example, a pH gradation of pH 10.
  • the pH gradation of the liquid 18 in the vicinity of the region 52 is, for example, the isoelectric point of pH 10 of the sample 4 (the fourth isoelectric point PH corresponding to 10) (an example of a fourth pH).
  • the surface area of MgO in contact with the liquid 18 decreases as the area ratio decreases, so the OH groups present on the surface of MgO are liquid The amount of hydrogen ions (H + ) in 18 is reduced. Therefore, the pH gradation formed by the area 52 is higher than the pH gradation formed by the area 50.
  • the plurality of metal oxide films 26 to 38 are arranged such that seven pH gradations of pH 4 to pH 10 are arranged in ascending order from the electrode 12 to the electrode 10. As a result, a pH gradient is formed on the electrophoresis support 8 along the longitudinal direction of the carrier 20, in which seven pH gradations of pH 4 to pH 10 are aligned in this order.
  • Each of the plurality of metal oxide films 26 to 38 described above is formed, for example, by using an ALD (Atomic Layer Deposition) method.
  • ALD Atomic Layer Deposition
  • each of the plurality of metal oxide films 26 to 38 can be formed into a desired shape.
  • the plurality of metal oxide films 26 to 38 are not only deposited on the plurality of regions 40 to 52 on the upper surface 24 of the carrier 20 but also penetrate the inside of the carrier 20 corresponding to the plurality of regions 40 to 52 respectively. .
  • the electrophoresis support 8 is disposed inside the container 6, and the liquid 18 is injected into the inside of the container 6.
  • the sample 4 is injected into one end (end on the electrode 12 side) in the longitudinal direction of the carrier 20.
  • a predetermined DC voltage is applied between the pair of electrodes 10 and 12 by the power supply device 14. For example, after applying a DC voltage of 500 V between the pair of electrodes 10 and 12 for one minute, the voltage value is raised to 3500 V in one and a half hours. Thereafter, a DC voltage of 3500 V is applied for six and a half hours between the pair of electrodes 10 and 12. Thereby, an electric field is formed between the pair of electrodes 10 and 12, and the sample 4 moves (migrates) inside the carrier 20 under the action of the electric field.
  • the movement distance of the sample 4 differs depending on the isoelectric point of the sample 4.
  • the protein 4 a moves to the position of the region 40 forming a pH gradation of pH 4.
  • the sample 4b moves to the position of the region 42 forming a pH gradation of pH5.
  • the position of the separated sample 4 can be detected by staining the electrophoresis support 8.
  • a stain of the electrophoresis support 8 for example, silver stain or the like is used.
  • the sample 4 may be stained using a fluorescent dye.
  • the position of the separated sample 4 can be detected by irradiating the electrophoresis support 8 after the electrophoresis with excitation light and observing the fluorescence.
  • the electrophoresis support 8 may be irradiated with light such as ultraviolet light or near infrared light, and the transmitted light or the reflected light of the irradiated light may be detected.
  • the sample 4 containing a protein has the property of absorbing light of a specific wavelength. Therefore, in the case where the light irradiated to the electrophoresis support 8 is detected, the intensity of the detected light is weaker at the place where the sample 4 is located than at other places.
  • the position of the separated sample 4 can also be detected by such a method.
  • the area ratio occupied by the metal oxide film 26 in the region 40 of the carrier 20, the area ratio occupied by the metal oxide film 28 in the region 42 of the carrier 20, and the metal oxide film 30 occupied in the region 44 of the carrier 20 The area ratio is made different from each other.
  • one metal oxide TiO 2 which is smaller than the number of pH gradations is used to form three metal oxide films 26, 28 and 30.
  • a film can be formed.
  • the area ratio occupied by the metal oxide film 34 in the region 48 of the carrier 20, the area ratio occupied by the metal oxide film 36 in the region 50 of the carrier 20, and the metal oxide film in the region 52 of the carrier 20 is different from one another.
  • three metal oxide films 34, 36 and 38 are formed using one kind of metal oxide MgO smaller than the number of pH gradations. It can be membrane.
  • the films 26 to 38 can be formed.
  • an increase in the number of steps for forming the plurality of metal oxide films 26 to 38 can be suppressed, and the plurality of metal oxide films 26 can be suppressed.
  • FIG. 4 is a top view showing the configuration of the metal oxide film 22A of the electrophoresis support 8A according to the second embodiment.
  • the same components as those in the first embodiment are denoted by the same reference numerals, and the description thereof is omitted.
  • the configuration of the metal oxide film 22A is different from that of the first embodiment.
  • the section length X1 of the area 40 of the carrier 20 is longer than the section length X2 of the area 42A of the carrier 20 and shorter than the section length X3 of the area 44A of the carrier 20.
  • the section length X1 of the area 52 of the carrier 20 is longer than the section length X2 of the area 50A of the carrier 20 and shorter than the section length X3 of the area 48A of the carrier 20.
  • the section lengths X1 of the regions 40, 46 and 52 of the carrier 20 are the same.
  • the number of the plurality of metal oxide film pieces 56 of the metal oxide film 30A is larger than the number of the plurality of metal oxide film pieces 54 of the metal oxide film 28A. Also, the diameter of each of the plurality of metal oxide film pieces 56 is smaller than the diameter of each of the plurality of metal oxide film pieces 54. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 30A in the region 44A of the carrier 20 is smaller than the area ratio occupied by the metal oxide film 28A in the region 42A of the carrier 20.
  • the number of the plurality of metal oxide film pieces 58 of the metal oxide film 34A is larger than the number of the plurality of metal oxide film pieces 60 of the metal oxide film 36A.
  • the diameter of each of the plurality of metal oxide film pieces 58 is smaller than the diameter of each of the plurality of metal oxide film pieces 60. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 34A in the region 48A of the carrier 20 is smaller than the area ratio occupied by the metal oxide film 36A in the region 50A of the carrier 20.
  • each section length X3 of the regions 44A and 48A of the carrier 20 is greater than the section lengths X1 and X2 Also make it longer.
  • proteins having isoelectric points of pH 6 and pH 8 can be separated with high precision by the metal oxide films 30A and 34A, respectively.
  • each section length X2 of the regions 42A and 50A of the carrier 20 is set to section length X1. It should be longer than this.
  • proteins having an isoelectric point of pH 5 and pH 9 can be separated with high precision by the metal oxide films 28A and 36A, respectively.
  • each section length X1 of the regions 40 and 52 of the carrier 20 is set to section length X2 And X3.
  • proteins having an isoelectric point of pH 4 and pH 10 can be separated with high precision by the metal oxide films 26 and 38, respectively.
  • FIG. 5 is a top view showing the configuration of the metal oxide film 22B of the electrophoresis support 8B according to the third embodiment.
  • metal oxide film body 22B includes metal oxide film 62 (an example of a fifth metal oxide film) and metal oxide film 64 (sixth metal oxide film) in addition to the plurality of metal oxide films 26 to 38.
  • metal oxide film 62 an example of a fifth metal oxide film
  • metal oxide film 64 ixth metal oxide film
  • a region 66 is disposed between the region 42 of the carrier 20 and the region 44
  • a region 68 is disposed between the region 48 and the region 50 of the carrier 20.
  • An example is arranged.
  • the metal oxide film 62 includes a plurality of metal oxide film pieces 70 (an example of a first metal oxide film piece) and a plurality of metal oxide film pieces 72 (a second metal) which are scattered in a lattice shape in the region 66 of the carrier 20. (An example of an oxide film piece).
  • each of the plurality of metal oxide film pieces 70 is arranged so as not to overlap with each of the plurality of metal oxide film pieces 72.
  • Each of the plurality of metal oxide film pieces 70 is formed of TiO 2
  • each of the plurality of metal oxide film pieces 72 is formed of MgO. That is, the metal oxide film 62 is formed of two different metal oxides.
  • Each of the plurality of metal oxide film pieces 70 and 72 is formed in a circular shape in plan view.
  • the number of each of the plurality of metal oxide film pieces 70 is greater than the number of each of the plurality of metal oxide film pieces 72.
  • the diameter of each of the plurality of metal oxide film pieces 70 is larger than the diameter of each of the plurality of metal oxide film pieces 72.
  • the diameter of each of the plurality of metal oxide film pieces 70 is the same as the diameter of each of the plurality of metal oxide film pieces 54.
  • Region 66 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 70, and a plurality of metal oxide film pieces 72.
  • the metal oxide film 62 affects the pH gradation in the region 66. Therefore, the pH gradation of the region 66 is the pH gradation (for example, pH 4) formed by the plurality of metal oxide film pieces 70, the pH gradation (for example, pH 7) formed by the plurality of metal oxide film pieces 72, and pure water Is formed in combination with the pH gradation (for example, pH 7) which is formed.
  • the pH gradation of the liquid 18 (see FIG. 1) in the vicinity of the region 66 corresponds to the pH 5.5 (the example of the fifth isoelectric point) of the pH 5.5 of the sample 4 Of the pH of
  • the OH group present on the surface of the metal oxide film 62 is a hydrogen ion in the liquid 18 (compared to the metal oxide film 28 formed only of TiO 2 ).
  • the amount of receiving H + ) increases. Therefore, the pH gradation formed by the area 66 is more alkaline (for example, pH 5.5) than the pH gradation formed by the area 42.
  • the metal oxide film 64 includes a plurality of metal oxide film pieces 74 (an example of a third metal oxide film piece) and a plurality of metal oxide film pieces 76 (a fourth metal) which are scattered in a lattice shape in the region 68 of the carrier 20. (An example of an oxide film piece).
  • each of the plurality of metal oxide film pieces 74 is arranged so as not to overlap with each of the plurality of metal oxide film pieces 76.
  • Each of the plurality of metal oxide film pieces 74 is formed of TiO 2
  • each of the plurality of metal oxide film pieces 76 is formed of MgO. That is, the metal oxide film 64 is formed of two different metal oxides.
  • Each of the plurality of metal oxide film pieces 74 and 76 is formed in a circular shape in plan view.
  • the number of each of the plurality of metal oxide film pieces 74 is smaller than the number of each of the plurality of metal oxide film pieces 76.
  • the diameter of each of the plurality of metal oxide film pieces 74 is smaller than the diameter of each of the plurality of metal oxide film pieces 76.
  • the diameter of each of the plurality of metal oxide film pieces 76 is the same as the diameter of each of the plurality of metal oxide film pieces 60.
  • Region 68 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 74, and a plurality of metal oxide film pieces 76.
  • the metal oxide film 64 affects the pH gradation in the region 68. Therefore, the pH gradation of the region 68 includes pH gradation (for example, pH 4) formed by the plurality of metal oxide film pieces 74, pH gradation (for example, 10 pH) formed by the plurality of metal oxide film pieces 76, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed.
  • the pH gradation of the liquid 18 in the vicinity of the region 68 is, for example, one example of the pH 8.5 (corresponding to one example of the sixth isoelectric point) of pH 8.5 of the sample 4 ).
  • the OH group present on the surface of the metal oxide film 64 is a hydrogen ion (H (H) as compared to the metal oxide film 36 formed only of MgO.
  • H (H) hydrogen ion
  • the amount of releasing + ) increases. Therefore, the pH gradation formed by the region 68 is more acidic (for example, pH 8.5) than the pH gradation formed by the region 50.
  • the number of each of the plurality of metal oxide film pieces 70 is larger than the number of each of the plurality of metal oxide film pieces 74, and the area of each of the plurality of metal oxide film pieces 70 is a plurality of It is larger than the area of each of the metal oxide film pieces 74.
  • the number of each of the plurality of metal oxide film pieces 72 is smaller than the number of each of the plurality of metal oxide film pieces 76, and the area of each of the plurality of metal oxide film pieces 72 is a plurality of metal oxide film pieces 76. Less than the area of each of Note that at least one of the area and the number may be different between each of the plurality of metal oxide film pieces 70 and each of the plurality of metal oxide film pieces 74. Similarly, at least one of the area and the number of each of the plurality of metal oxide film pieces 72 and each of the plurality of metal oxide film pieces 76 may be different.
  • each of the plurality of metal oxide film pieces 70 of the metal oxide film 62 is arranged so as not to overlap with each of the plurality of metal oxide film pieces 72.
  • a contact area between each of the metal oxide film pieces 70 and the plurality of metal oxide film pieces 72 and the liquid 18 can be sufficiently secured.
  • the redox reaction of the plurality of metal oxide film pieces 70 and the plurality of metal oxide film pieces 72 with respect to the liquid 18 can be efficiently generated.
  • Embodiment 4 [4-1. Configuration of metal oxide film]
  • FIG. 6 is a top view showing the configuration of the metal oxide film 22C of the electrophoresis support 8C according to the fourth embodiment.
  • FIG. 7 is a cross-sectional view of an essential part of the electrophoresis support 8C according to the fourth embodiment, taken along the line VII-VII in FIG.
  • FIG. 8 is a cross-sectional view of an essential portion of the electrophoresis support 8C according to the fourth embodiment, taken along line VIII-VIII in FIG.
  • the configuration of the metal oxide film 22C is different from that of the first embodiment.
  • the metal oxide film body 22 C has metal oxide films 78 and 80 (an example of a fifth metal oxide film) in addition to the plurality of metal oxide films 26 to 38.
  • a region 82 is disposed between the region 42 of the carrier 20 and the region 44
  • a region 84 is disposed between the region 48 and the region 50 of the carrier 20.
  • An example is arranged.
  • the metal oxide film 78 includes a plurality of metal oxide film pieces 86 (an example of a first metal oxide film piece) and a plurality of metal oxide film pieces 88 (a second metal) scattered in a lattice shape in the region 82 of the carrier 20. (An example of an oxide film piece). As shown in FIGS. 6 and 7, each of the plurality of metal oxide film pieces 86 is arranged to overlap with each of the plurality of metal oxide film pieces 88 when the carrier 20 is viewed in plan.
  • Each of the plurality of metal oxide film pieces 86 is formed of TiO 2
  • each of the plurality of metal oxide film pieces 88 is formed of MgO. That is, the metal oxide film 78 is formed of two different metal oxides.
  • Each of the plurality of metal oxide film pieces 86 and 88 is formed in a circular shape in plan view. The number and diameter of each of the plurality of metal oxide film pieces 86 are the same as the number and diameter of each of the plurality of metal oxide film pieces 88, respectively. As shown in FIG. 7, each of the plurality of metal oxide film pieces 86 is stacked on the upper side of each of the plurality of metal oxide film pieces 88.
  • Region 82 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 86, and a plurality of metal oxide film pieces 88.
  • the metal oxide film 78 affects the pH gradation in the region 82. Therefore, the pH gradation of the region 82 includes pH gradation (for example, pH 4) formed by the plurality of metal oxide film pieces 86, pH gradation (for example, 7 pH) formed by the plurality of metal oxide film pieces 88, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed.
  • the pH gradation of the liquid 18 (see FIG. 1) in the vicinity of the region 82 is, for example, pH 5.5 (the fifth isoelectric point) corresponding to the pH 5.5 of the sample 4 An example of the pH of 5).
  • the metal oxide film 80 includes a plurality of metal oxide film pieces 90 (an example of a first metal oxide film piece) and a plurality of metal oxide film pieces 92 (a second metal) which are scattered in a lattice shape in the region 84 of the carrier 20. (An example of an oxide film piece). As shown in FIGS. 6 and 8, each of the plurality of metal oxide film pieces 90 is arranged to overlap with each of the plurality of metal oxide film pieces 92 when the carrier 20 is viewed in plan.
  • Each of the plurality of metal oxide film pieces 90 is formed of TiO 2
  • each of the plurality of metal oxide film pieces 92 is formed of MgO. That is, the metal oxide film 80 is formed of two different metal oxides.
  • Each of the plurality of metal oxide film pieces 90 and 92 is formed in a circular shape in plan view. The number and diameter of each of the plurality of metal oxide film pieces 90 is the same as the number and diameter of each of the plurality of metal oxide film pieces 92. As shown in FIG. 8, each of the plurality of metal oxide film pieces 92 is stacked on the upper side of each of the plurality of metal oxide film pieces 90.
  • Region 84 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 90, and a plurality of metal oxide film pieces 92.
  • the metal oxide film 80 affects the pH gradation in the region 84. Therefore, the pH gradation of the region 84 includes pH gradation (for example, pH 4) formed by the plurality of metal oxide film pieces 90, pH gradation (for example, pH 10) formed by the plurality of metal oxide film pieces 92, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed.
  • the pH gradation of the liquid 18 in the vicinity of the region 84 is, for example, one example of the pH 8.5 (corresponding to one example of the fifth isoelectric point) of pH 8.5 of the sample 4 ).
  • the pH gradation of the pH gradient can be further subdivided by forming each of the metal oxide films 78 and 80 with two different metal oxides.
  • each of the plurality of metal oxide film pieces 86 of the metal oxide film 78 is disposed so as to overlap with each of the plurality of metal oxide film pieces 88 when the carrier 20 is viewed in a plan view. Even if the area of the region 82 can not be sufficiently secured, the metal oxide film 78 can be compactly disposed in the region 82.
  • FIG. 9 is a top view showing the structure of a metal oxide film body 22D of an electrophoresis support 8D according to the fifth embodiment.
  • FIG. 10 is a view for explaining an example of the film forming step of the metal oxide film 22D of the electrophoresis support 8D according to the fifth embodiment.
  • FIG. 11 is a graph for explaining the pH gradient formed by the metal oxide film body 22D of the electrophoresis support 8D according to the fifth embodiment.
  • the configuration of the metal oxide film 22D is different from that of the first embodiment.
  • the metal oxide film body 22D has a plurality of metal oxide films 94 to 102.
  • the plurality of metal oxide films 94 to 102 are respectively disposed in the plurality of regions 104 to 112 on the upper surface 24 of the carrier 20.
  • the plurality of regions 104 to 112 are arranged in line in this order along the longitudinal direction (X-axis direction) of the carrier 20.
  • the metal oxide film 94 is formed of TiO 2 , and is uniformly disposed over the entire region 104 of the carrier 20.
  • the metal oxide film 94 forms, for example, a pH gradation of pH4.
  • the pH gradation of the liquid 18 (see FIG. 1) in the vicinity of the region 104 corresponds to, for example, the isoelectric point of pH 4 of the sample 4.
  • the metal oxide film 96 has a plurality of metal oxide film pieces 114 and a plurality of metal oxide film pieces 116 interspersed in a lattice shape in the region 106 of the carrier 20.
  • each of the plurality of metal oxide film pieces 114 is arranged to overlap with each of the plurality of metal oxide film pieces 116.
  • Each of the plurality of metal oxide film pieces 114 is formed of TiO 2
  • each of the plurality of metal oxide film pieces 116 is formed of MgO. That is, the metal oxide film 96 is formed of two different metal oxides.
  • Each of the plurality of metal oxide film pieces 114 and 116 is formed in a circular shape in plan view.
  • the number of each of the plurality of metal oxide film pieces 114 is the same as the number of each of the plurality of metal oxide film pieces 116.
  • the diameter of each of the plurality of metal oxide film pieces 114 is larger than the diameter of each of the plurality of metal oxide film pieces 116.
  • Each of the plurality of metal oxide film pieces 114 is stacked under each of the plurality of metal oxide film pieces 116.
  • Region 106 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 114 and a plurality of metal oxide film pieces 116.
  • the metal oxide film 96 affects the pH gradation in the region 106. Therefore, the pH gradation of the region 106 includes pH gradation (for example, pH 4) formed by a plurality of metal oxide film pieces 114, pH gradation (for example, pH 7) formed by a plurality of metal oxide film pieces 116, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thereby, the pH gradation of the liquid 18 in the vicinity of the region 106 becomes, for example, pH 5.5 corresponding to the isoelectric point of pH 5.5 of the sample 4.
  • pH gradation for example, pH 4
  • the metal oxide film 98 has a plurality of metal oxide film pieces 118 and a plurality of metal oxide film pieces 120 interspersed in a lattice shape in the region 108 of the carrier 20.
  • each of the plurality of metal oxide film pieces 118 is arranged to overlap with each of the plurality of metal oxide film pieces 120.
  • Each of the plurality of metal oxide film pieces 118 is formed of TiO 2
  • each of the plurality of metal oxide film pieces 120 is formed of MgO. That is, the metal oxide film 98 is formed of two different metal oxides.
  • Each of the plurality of metal oxide film pieces 118 and 120 is formed in a circular shape in plan view. The number and diameter of each of the plurality of metal oxide film pieces 118 are the same as the number and diameter of each of the plurality of metal oxide film pieces 120.
  • Each of the plurality of metal oxide film pieces 118 is stacked under each of the plurality of metal oxide film pieces 120.
  • Region 108 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 118, and a plurality of metal oxide film pieces 120.
  • the metal oxide film 98 affects the pH gradation in the region 108. Therefore, the pH gradation of the region 108 includes pH gradation (for example, pH 4) formed by a plurality of metal oxide film pieces 118, pH gradation (for example, pH 7) formed by a plurality of metal oxide film pieces 120, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thereby, the pH gradation of the liquid 18 in the vicinity of the region 108 becomes, for example, pH 7 corresponding to the isoelectric point of pH 7 of the sample 4.
  • pH gradation for example, pH 4
  • the metal oxide film 100 has a plurality of metal oxide film pieces 122 and a plurality of metal oxide film pieces 124 scattered in a lattice shape in the region 110 of the carrier 20.
  • each of the plurality of metal oxide film pieces 122 is arranged to overlap with each of the plurality of metal oxide film pieces 124.
  • Each of the plurality of metal oxide film pieces 122 is formed of TiO 2
  • each of the plurality of metal oxide film pieces 124 is formed of MgO. That is, the metal oxide film 100 is formed of two different metal oxides.
  • Each of the plurality of metal oxide film pieces 122 and 124 is formed in a circular shape in plan view.
  • the number of each of the plurality of metal oxide film pieces 122 is the same as the number of each of the plurality of metal oxide film pieces 124.
  • the diameter of each of the plurality of metal oxide film pieces 122 is smaller than the diameter of each of the plurality of metal oxide film pieces 124.
  • Each of the plurality of metal oxide film pieces 122 is stacked under each of the plurality of metal oxide film pieces 124.
  • the region 110 is formed by a portion where the carrier 20 is exposed, a plurality of metal oxide film pieces 122, and a plurality of metal oxide film pieces 124.
  • the metal oxide film 100 affects the pH gradation in the region 110. Therefore, the pH gradation of the region 110 includes pH gradation (for example, pH 4) formed by a plurality of metal oxide film pieces 122, pH gradation (for example, pH 10) formed by a plurality of metal oxide film pieces 124, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thereby, the pH gradation of the liquid 18 in the vicinity of the region 110 becomes, for example, pH 8.5 corresponding to the isoelectric point of pH 8.5 of the sample 4.
  • pH gradation for example, pH 4
  • the metal oxide film 102 is formed of MgO and is uniformly disposed over the entire region 112 of the carrier 20.
  • the metal oxide film 102 forms, for example, a pH gradation of pH 10.
  • the pH gradation of the liquid 18 in the vicinity of the region 112 becomes, for example, pH 10 corresponding to the isoelectric point of pH 10 of the sample 4.
  • a 1st film-forming process is performed.
  • an organometallic compound gas of Ti is introduced by the ALD method.
  • the metal mask 126 includes a region 130a in which the rectangular holes 128a are formed, a region 130b in which the plurality of circular holes 128b are formed, a region 130c in which the plurality of circular holes 128c is formed, and a circular shape
  • a plurality of holes 128d are formed in a region 130d and a closed region 130e.
  • Each of the plurality of holes 128b, the plurality of holes 128c, and the plurality of holes 128d is arranged in a lattice.
  • the numbers of the plurality of holes 128b, the plurality of holes 128c, and the plurality of holes 128d are the same.
  • the diameter of each of the plurality of holes 128b is larger than the diameter of each of the plurality of holes 128c.
  • the diameter of each of the plurality of holes 128c is larger than the diameter of each of the plurality of holes 128d.
  • the metal oxide film 94 is formed in the region 104 of the carrier 20 by the organometallic compound gas of Ti introduced through the holes 128a of the metal mask 126. Further, a plurality of metal oxide film pieces 114 are formed in the region 106 of the carrier 20 by the metal organic metal compound gas of Ti introduced through the plurality of holes 128 b of the metal mask 126. Further, a plurality of metal oxide film pieces 118 are formed in the region 108 of the carrier 20 by the metal organic metal compound gas of Ti introduced through the plurality of holes 128 c of the metal mask 126.
  • a plurality of metal oxide film pieces 122 are formed in the region 110 of the carrier 20 by the organometallic compound gas of Ti introduced through the plurality of holes 128 d of the metal mask 126. Since the region 112 of the carrier 20 is completely covered by the region 130e of the metal mask 126, the metal oxide film of TiO 2 is not formed.
  • a second film forming process is performed.
  • an organic metal compound gas of Mg is introduced by the ALD method.
  • the direction of the metal mask 126 is reversed from the first film forming process.
  • the metal oxide film 102 is formed in the region 112 of the carrier 20 by the Mg organic metal compound gas introduced through the holes 128a of the metal mask 126.
  • a plurality of metal oxide film pieces 124 are stacked on the plurality of metal oxide film pieces 122 by metal organic metal compound gas introduced through the plurality of holes 128b of the metal mask 126.
  • the plurality of metal oxide film pieces 120 are stacked on the plurality of metal oxide film pieces 118 by the metal organic metal compound gas of Mg introduced through the plurality of holes 128c of the metal mask 126.
  • a plurality of metal oxide film pieces 116 are stacked on the plurality of metal oxide film pieces 114 in the region 106 of the carrier 20 by the metal organic metal compound gas of Mg introduced through the plurality of holes 128 d of the metal mask 126. Formed to Since the region 104 of the carrier 20 is completely covered with the region 130e of the metal mask 126, a metal oxide film of MgO is not formed.
  • the plurality of metal oxide films 94 to 102 are formed on the plurality of regions 104 to 112 of the carrier 20, respectively.
  • the pH of pH 4 to pH 7 formed by the metal oxide film 94 of TiO 2 and the plurality of metal oxide film pieces 114, 118 and 122 As shown in FIG. 11, in the metal oxide film body 22D formed as described above, the pH of pH 4 to pH 7 formed by the metal oxide film 94 of TiO 2 and the plurality of metal oxide film pieces 114, 118 and 122.
  • the gradient and the pH gradient of pH 7 to pH 10 formed by the metal oxide film 102 of MgO and the plurality of metal oxide film fragments 116, 120 and 124 mutually influence to form a pH gradient of pH 4 to pH 10 Become so.
  • the metal oxide film 22 includes at least two of TiO 2 , NiO and MgO, but it is possible to include metal oxides other than the above. Good.
  • the metal oxide film body 22 (22A, 22B, 22C, 22D) is, for example, Al 2 O 3 , HfO, WO 3 , La 2 O 3 , Bi 2 O 3 , SnO 2 , ZnO, In 2 O, Fe 3 O 4 And any two or more metal oxides such as CuO and SiO 2 may be included.
  • the sample 4 contains a protein, but it may contain, for example, DNA (deoxyribonucleic acid) or the like.
  • the present invention is not limited to this, for example, any number of pH gradations such as nine pH gradations of pH 3 to pH 11 You may form a pH gradient with
  • each component may be configured by dedicated hardware or may be realized by executing a software program suitable for each component.
  • Each component may be realized by a program execution unit such as a CPU or a processor reading out and executing a software program recorded on a recording medium such as a hard disk or a semiconductor memory.
  • Each of the above-described devices can be specifically realized by a computer system including a microprocessor, a ROM, a RAM, a hard disk unit, a display unit, a keyboard, a mouse and the like.
  • a computer program is stored in the RAM or the hard disk unit.
  • Each device achieves its function by the microprocessor operating according to the computer program.
  • the computer program is configured by combining a plurality of instruction codes indicating instructions to the computer in order to achieve a predetermined function.
  • the system LSI is a super-multifunctional LSI manufactured by integrating a plurality of components on one chip, and more specifically, a computer system including a microprocessor, a ROM, a RAM, and the like. .
  • a computer program is stored in the ROM.
  • the system LSI achieves its functions as the microprocessor loads a computer program from the ROM to the RAM and operates according to the loaded computer program.
  • a part or all of the components constituting each of the above-described devices may be configured from an IC card or a single module that can be detached from each device.
  • the IC card or module is a computer system including a microprocessor, a ROM, a RAM, and the like.
  • the IC card or module may include the above-described ultra-multifunctional LSI.
  • the IC card or module achieves its function by the microprocessor operating according to the computer program. This IC card or this module may have tamper resistance.
  • the present invention may be realized by the method shown above. Also, these methods may be realized by a computer program realized by a computer, or may be realized by a digital signal consisting of a computer program.
  • the present invention is a computer program or a recording medium which can read digital signals from a computer, such as a flexible disk, a hard disk, a CD-ROM, an MO, a DVD, a DVD-ROM, a DVD-RAM, a BD (Blu-ray (registered trademark) (Disc), a semiconductor memory or the like. Also, it may be realized by digital signals recorded in these recording media.
  • the present invention may transmit a computer program or a digital signal via a telecommunication line, a wireless or wired communication line, a network represented by the Internet, data broadcasting, and the like.
  • the present invention is a computer system comprising a microprocessor and a memory, the memory storing a computer program, and the microprocessor may operate according to the computer program.
  • the electrophoresis support of the present invention can be applied to an electrophoresis apparatus or the like for electrophoresis of a sample.
  • electrophoresis apparatus 4 samples 4a, 4b protein 6 containers 8, 8A, 8B, 8C, 8D electrophoresis support 10, 12 electrode 14 power supply 16 opening 18 liquid 20 carrier 22, 22A, 22B, 22C, 22D metal oxidation Upper surface 26, 28, 28A, 30, 30A, 32, 34, 34A, 36, 36A, 38, 62, 64, 78, 94, 96, 98, 100, 102 Metal oxide films 40, 42, 42A, 44, 44A, 46, 48, 48A, 50, 50A, 52, 66, 68, 82, 84, 106, 108, 110, 112, 130a, 130b, 130c, 130d, 130e regions 54, 56, 58, 60, 70, 72, 74, 76, 68, 88, 90, 92, 114, 116, 118, 120, 122, 124 metal oxide film pieces 1 6 metal mask 128a, 128b, 128c, 128d hole

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Abstract

This electrophoretic support (8) is provided with: a carrier (20) formed in a porous shape and having an upper surface (24) on which a region (40) and a region (42) disposed adjacent to the region (40)are formed; a metal oxide film (26) for forming a first pH that corresponds to a first isoelectric point of a sample (4), the metal oxide film (26) being disposed in the region (40) and composed of a first metal oxide; and a metal oxide film (28) for forming a second pH that corresponds to a second isoelectric point of the sample (4), the metal oxide film (28) being disposed in the region (42) and composed of the first metal oxide. When viewing the carrier (20) in a plan view, a first surface area ratio occupied by the metal oxide film (26) in the region (40) is different from a second surface area ratio occupied by the metal oxide film (28) in the region (42).

Description

電気泳動支持体及び電気泳動装置Electrophoresis support and electrophoresis apparatus
　本発明は、電気泳動支持体及びこれを備えた電気泳動装置に関する。 The present invention relates to an electrophoresis support and an electrophoresis apparatus provided with the same.
　試料の等電点の違いを利用して、試料を分離する等電点電気泳動が知られている。等電点電気泳動では、電気泳動装置が用いられる（例えば、特許文献１参照）。この電気泳動装置は、容器と、容器の内部に配置された一対の電極と、一対の電極の間に配置された電気泳動支持体とを備えている。 Isoelectric focusing electrophoresis is known in which a sample is separated by utilizing the difference in the isoelectric point of the sample. In isoelectric focusing, an electrophoresis apparatus is used (see, for example, Patent Document 1). The electrophoresis apparatus includes a container, a pair of electrodes disposed inside the container, and an electrophoresis support disposed between the pair of electrodes.
　電気泳動支持体は、多孔質状の担体と、担体の上面に一列に並んで配置された複数の金属酸化膜とを有している。複数の金属酸化膜はそれぞれ、互いに異なる種類の複数の金属酸化物で形成されている。これらの複数の金属酸化膜により、担体にｐＨ勾配が形成される。 The electrophoresis support has a porous carrier and a plurality of metal oxide films arranged in a line on the top surface of the carrier. Each of the plurality of metal oxide films is formed of a plurality of metal oxides of different types. The plurality of metal oxide films form a pH gradient on the carrier.
国際公開第２０１６／１６３０９７号International Publication No. 2016/163097
　上述した従来の電気泳動支持体では、１つのｐＨ階調を形成するためには１種類の金属酸化物が必要となる。そのため、例えばｐＨ４～ｐＨ１０の７つのｐＨ階調を有するｐＨ勾配を形成する場合には、ｐＨ階調と同数の７種類の金属酸化物が必要になる。これにより、ｐＨ階調を増やすに従って、複数の金属酸化膜の成膜工数が増大するとともに、複数の金属酸化膜の成膜制御が複雑になる。その結果、電気泳動支持体の製造プロセスが複雑になるという課題が生じる。 The conventional electrophoretic support described above requires one type of metal oxide to form one pH gradation. Therefore, in the case of forming a pH gradient having seven pH gradations, for example, pH 4 to pH 10, seven types of metal oxides equal in number to the pH gradation are required. As a result, as the pH gradation is increased, the number of steps for forming the plurality of metal oxide films increases, and the control of forming the plurality of metal oxide films becomes complicated. As a result, there arises a problem that the manufacturing process of the electrophoresis support becomes complicated.
　そこで、本発明は、ｐＨ勾配のｐＨ階調の数を増やした場合であっても、製造プロセスが複雑になるのを抑制することができる電気泳動支持体及びこれを備えた電気泳動装置を提供する。 Therefore, the present invention provides an electrophoresis support capable of suppressing the manufacturing process from becoming complicated even when the number of pH gradations in the pH gradient is increased, and an electrophoresis apparatus including the same. Do.
　本発明の一態様に係る電気泳動支持体は、試料を電気泳動するための電気泳動装置に用いられる電気泳動支持体であって、多孔質状に形成された担体であって、第１の領域と、前記第１の領域に隣接して配置された第２の領域とが表面に形成された担体と、前記試料の第１の等電点に対応する第１のｐＨを形成するための第１の金属酸化膜であって、前記第１の領域に配置され、第１の金属酸化物で形成された第１の金属酸化膜と、前記試料の第２の等電点に対応する第２のｐＨを形成するための第２の金属酸化膜であって、前記第２の領域に配置され、前記第１の金属酸化物で形成された第２の金属酸化膜と、を備え、前記担体を平面視した際に、前記第１の領域において前記第１の金属酸化膜が占める第１の面積割合は、前記第２の領域において前記第２の金属酸化膜が占める第２の面積割合と異なる。 An electrophoresis support according to an aspect of the present invention is an electrophoresis support used in an electrophoresis apparatus for electrophoresis of a sample, which is a porous carrier, and includes a first region A carrier having a surface formed thereon, and a second region disposed adjacent to the first region, and a first for forming a first pH corresponding to a first isoelectric point of the sample A first metal oxide film disposed in the first region and formed of a first metal oxide, and a second metal oxide film corresponding to a second isoelectric point of the sample A second metal oxide film for forming the pH of the second metal oxide film, the second metal oxide film disposed in the second region and formed of the first metal oxide, and the carrier And the first area ratio occupied by the first metal oxide film in the first region is the second region. Different from the second area ratio occupied the second metal oxide film is in the.
　なお、これらの包括的又は具体的な態様は、システム、方法、集積回路、コンピュータプログラム又はコンピュータ読み取り可能なＣＤ－ＲＯＭなどの記録媒体で実現されてもよく、システム、方法、集積回路、コンピュータプログラム及び記録媒体の任意な組み合わせで実現されてもよい。 Note that these general or specific aspects may be realized by a system, a method, an integrated circuit, a computer program, or a recording medium such as a computer readable CD-ROM, a system, a method, an integrated circuit, a computer program And any combination of recording media.
　本発明の一態様に係る電気泳動支持体等では、ｐＨ勾配のｐＨ階調の数を増やした場合であっても、製造プロセスが複雑になるのを抑制することができる。 In the electrophoresis support or the like according to one aspect of the present invention, the manufacturing process can be prevented from becoming complicated even when the number of pH gradations is increased.
図１は、実施の形態１に係る電気泳動装置の構成を示す上面図である。FIG. 1 is a top view showing the configuration of the electrophoresis apparatus according to the first embodiment. 図２は、図１のＩＩ－ＩＩ線による、実施の形態１に係る電気泳動装置の断面図である。FIG. 2 is a cross-sectional view of the electrophoresis device according to the first embodiment, taken along line II-II in FIG. 図３は、実施の形態１に係る電気泳動支持体の金属酸化膜体の構成を示す上面図である。FIG. 3 is a top view showing the structure of the metal oxide film of the electrophoresis support according to the first embodiment. 図４は、実施の形態２に係る電気泳動支持体の金属酸化膜体の構成を示す上面図である。FIG. 4 is a top view showing the configuration of the metal oxide film of the electrophoresis support according to the second embodiment. 図５は、実施の形態３に係る電気泳動支持体の金属酸化膜体の構成を示す上面図である。FIG. 5 is a top view showing the configuration of the metal oxide film of the electrophoresis support according to the third embodiment. 図６は、実施の形態４に係る電気泳動支持体の金属酸化膜体の構成を示す上面図である。FIG. 6 is a top view showing the configuration of the metal oxide film of the electrophoresis support according to the fourth embodiment. 図７は、図６のＶＩＩ－ＶＩＩ線による、実施の形態４に係る電気泳動支持体の要部断面図である。FIG. 7 is a cross-sectional view of an essential part of the electrophoresis support according to the fourth embodiment, taken along the line VII-VII in FIG. 図８は、図６のＶＩＩＩ－ＶＩＩＩ線による、実施の形態４に係る電気泳動支持体の要部断面図である。FIG. 8 is a cross-sectional view of essential parts of the electrophoresis support according to the fourth embodiment, taken along line VIII-VIII in FIG. 図９は、実施の形態５に係る電気泳動支持体の金属酸化膜体の構成を示す上面図である。FIG. 9 is a top view showing the configuration of the metal oxide film of the electrophoresis support according to the fifth embodiment. 図１０は、実施の形態５に係る電気泳動支持体の金属酸化膜体の成膜工程の一例を説明するための図である。FIG. 10 is a view for explaining an example of the film formation step of the metal oxide film of the electrophoresis support according to the fifth embodiment. 図１１は、実施の形態５に係る電気泳動支持体の金属酸化膜体により形成されるｐＨ勾配を説明するためのグラフである。FIG. 11 is a graph for explaining the pH gradient formed by the metal oxide film of the electrophoresis support according to the fifth embodiment.
　本発明の一態様に係る電気泳動支持体は、試料を電気泳動するための電気泳動装置に用いられる電気泳動支持体であって、多孔質状に形成された担体であって、第１の領域と、前記第１の領域に隣接して配置された第２の領域とが表面に形成された担体と、前記試料の第１の等電点に対応する第１のｐＨを形成するための第１の金属酸化膜であって、前記第１の領域に配置され、第１の金属酸化物で形成された第１の金属酸化膜と、前記試料の第２の等電点に対応する第２のｐＨを形成するための第２の金属酸化膜であって、前記第２の領域に配置され、前記第１の金属酸化物で形成された第２の金属酸化膜と、を備え、前記担体を平面視した際に、前記第１の領域において前記第１の金属酸化膜が占める第１の面積割合は、前記第２の領域において前記第２の金属酸化膜が占める第２の面積割合と異なる。 An electrophoresis support according to an aspect of the present invention is an electrophoresis support used in an electrophoresis apparatus for electrophoresis of a sample, which is a porous carrier, and includes a first region A carrier having a surface formed thereon, and a second region disposed adjacent to the first region, and a first for forming a first pH corresponding to a first isoelectric point of the sample A first metal oxide film disposed in the first region and formed of a first metal oxide, and a second metal oxide film corresponding to a second isoelectric point of the sample A second metal oxide film for forming the pH of the second metal oxide film, the second metal oxide film disposed in the second region and formed of the first metal oxide, and the carrier And the first area ratio occupied by the first metal oxide film in the first region is the second region. Different from the second area ratio occupied the second metal oxide film is in the.
　本態様によれば、担体を平面視した際に、第１の領域において第１の金属酸化膜が占める第１の面積割合は、第２の領域において第２の金属酸化膜が占める第２の面積割合と異なる。これにより、ｐＨ勾配において複数のｐＨ階調を形成する場合であっても、ｐＨ階調の数よりも少ない種類の金属酸化物を用いて、第１の金属酸化膜及び第２の金属酸化膜を含む複数の金属酸化膜を成膜することができる。その結果、ｐＨ勾配のｐＨ階調の数を増やした場合であっても、複数の金属酸化膜の成膜工数が増大するのを抑制することができるとともに、複数の金属酸化膜の成膜制御を簡素化することができる。したがって、電気泳動支持体の製造プロセスが複雑になるのを抑制することができる。 According to this aspect, when the carrier is viewed in plan, the first area ratio occupied by the first metal oxide film in the first region is the second area occupied by the second metal oxide film in the second region. It is different from the area ratio. Thereby, even when a plurality of pH gradations are formed in the pH gradient, the first metal oxide film and the second metal oxide film are formed using metal oxides of a type smaller than the number of pH gradations. Can be formed into a plurality of metal oxide films. As a result, even when the number of pH gradations is increased, it is possible to suppress an increase in the number of steps for forming a plurality of metal oxide films, and to control the formation of a plurality of metal oxide films. Can be simplified. Therefore, the manufacturing process of the electrophoresis support can be suppressed from being complicated.
　例えば、前記担体の前記表面には、さらに、前記第２の領域を挟んで前記第１の領域と反対側に配置された第３の領域が形成され、前記電気泳動支持体は、さらに、前記試料の第３の等電点に対応する第３のｐＨを形成するための第３の金属酸化膜であって、前記第３の領域に配置され、第２の金属酸化物で形成された第３の金属酸化膜を備えるように構成してもよい。 For example, on the surface of the carrier, a third region is further formed opposite to the first region across the second region, and the electrophoresis support further includes: A third metal oxide film for forming a third pH corresponding to a third isoelectric point of a sample, wherein the third metal oxide film is disposed in the third region and formed of a second metal oxide You may comprise so that the metal oxide film of 3 may be provided.
　本態様によれば、ｐＨ勾配のｐＨ階調をより細分化することができる。 According to this aspect, the pH gradation of the pH gradient can be further subdivided.
　例えば、前記担体の前記表面には、さらに、前記第３の領域を挟んで前記第２の領域と反対側に配置された第４の領域が形成され、前記電気泳動支持体は、さらに、前記試料の第４の等電点に対応する第４のｐＨを形成するための第４の金属酸化膜であって、前記第４の領域に配置され、前記第２の金属酸化物で形成された第４の金属酸化膜を備え、前記担体を平面視した際に、前記第３の領域において前記第３の金属酸化膜が占める第３の面積割合は、前記第４の領域において前記第４の金属酸化膜が占める第４の面積割合と異なるように構成してもよい。 For example, on the surface of the carrier, a fourth region is further formed opposite to the second region across the third region, and the electrophoresis support further includes the fourth member. It is a 4th metal oxide film for forming the 4th pH corresponding to the 4th isoelectric point of a sample, Comprising: It arrange | positioned in the said 4th area | region and was formed with the said 2nd metal oxide. A fourth metal oxide film is provided, and the third area ratio occupied by the third metal oxide film in the third region when the carrier is viewed in plan is the fourth area ratio in the fourth region. It may be configured to be different from the fourth area ratio occupied by the metal oxide film.
　本態様によれば、ｐＨ勾配のｐＨ階調をより細分化することができる。 According to this aspect, the pH gradation of the pH gradient can be further subdivided.
　例えば、前記第１の等電点が前記第２の等電点よりも低く、且つ、前記第２の等電点が純水のｐＨに対応する等電点よりも低い場合には、前記第２の面積割合は、前記第１の面積割合よりも小さいように構成してもよい。 For example, when the first isoelectric point is lower than the second isoelectric point and the second isoelectric point is lower than the isoelectric point corresponding to the pH of pure water, the first isoelectric point is lower than the second isoelectric point. The area ratio of 2 may be configured to be smaller than the first area ratio.
　本態様によれば、第２の金属酸化膜により形成される第２のｐＨが第１の金属酸化膜により形成される第１のｐＨよりも高くなるように、ｐＨ勾配を形成することができる。 According to this aspect, the pH gradient can be formed such that the second pH formed by the second metal oxide film is higher than the first pH formed by the first metal oxide film. .
　例えば、前記第１の等電点が前記第２の等電点よりも高く、且つ、前記第２の等電点が純水のｐＨに対応する等電点よりも高い場合には、前記第２の面積割合は、前記第１の面積割合よりも小さいように構成してもよい。 For example, when the first isoelectric point is higher than the second isoelectric point, and the second isoelectric point is higher than the isoelectric point corresponding to the pH of pure water, the first isoelectric point is higher than the second isoelectric point. The area ratio of 2 may be configured to be smaller than the first area ratio.
　本態様によれば、第２の金属酸化膜により形成される第２のｐＨが第１の金属酸化膜により形成される第１のｐＨよりも低くなるように、ｐＨ勾配を形成することができる。 According to this aspect, it is possible to form a pH gradient such that the second pH formed by the second metal oxide film is lower than the first pH formed by the first metal oxide film. .
　例えば、前記担体の前記表面には、さらに、前記第２の領域と前記第３の領域との間に配置された第５の領域が形成され、前記電気泳動支持体は、さらに、前記試料の第５の等電点に対応する第５のｐＨを形成するための第５の金属酸化膜であって、前記第５の領域に配置され、前記第１の金属酸化物及び前記第２の金属酸化物で形成された第５の金属酸化膜を備えるように構成してもよい。 For example, a fifth region is further formed on the surface of the carrier, the fifth region being disposed between the second region and the third region, and the electrophoresis support further comprises: A fifth metal oxide film for forming a fifth pH corresponding to a fifth isoelectric point, which is disposed in the fifth region, and the first metal oxide and the second metal You may comprise so that the 5th metal oxide film formed with the oxide may be provided.
　本態様によれば、第１の金属酸化物及び第２の金属酸化物で形成された第５の金属酸化膜を、第１の金属酸化物で形成された第２の金属酸化膜と第２の金属酸化物で形成された第３の金属酸化膜との間に配置するので、ｐＨ勾配のｐＨ階調をより細分化することができる。 According to this aspect, the fifth metal oxide film formed of the first metal oxide and the second metal oxide is different from the second metal oxide film formed of the first metal oxide and the second metal oxide film. The third embodiment is disposed between the second metal oxide film and the third metal oxide film formed of the second metal oxide, so that pH gradation of the pH gradient can be further subdivided.
　例えば、前記第５の金属酸化膜は、前記第１の金属酸化物で形成され、前記第５の領域において点在する複数の第１の金属酸化膜片と、前記第２の金属酸化物で形成され、前記第５の領域において点在する複数の第２の金属酸化膜片と、を含み、前記担体を平面視した際に、前記複数の第１の金属酸化膜片の各々は、前記複数の第２の金属酸化膜片の各々と互いに重ならないように配置されているように構成してもよい。 For example, the fifth metal oxide film is formed of the first metal oxide, and includes a plurality of first metal oxide film pieces scattered in the fifth region and the second metal oxide. And a plurality of second metal oxide film pieces formed and scattered in the fifth region, and when the carrier is viewed in plan, each of the plurality of first metal oxide film pieces is The plurality of second metal oxide film pieces may be arranged not to overlap with each other.
　本態様によれば、担体を平面視した際に、複数の第１の金属酸化膜片の各々は、複数の第２の金属酸化膜片の各々と互いに重ならないように配置されているので、複数の第１の金属酸化膜片及び複数の第２の金属酸化膜片の各々と、純水等の液体との接触面積を十分に確保することができる。その結果、複数の第１の金属酸化膜片及び複数の第２の金属酸化膜片の各々の、液体に対する酸化還元反応を効率良く生じさせることができる。 According to this aspect, each of the plurality of first metal oxide film pieces is arranged so as not to overlap with each of the plurality of second metal oxide film pieces when the carrier is viewed in a plan view. A contact area between each of the plurality of first metal oxide film pieces and the plurality of second metal oxide film pieces and a liquid such as pure water can be sufficiently secured. As a result, each of the plurality of first metal oxide film pieces and the plurality of second metal oxide film pieces can efficiently generate the redox reaction to the liquid.
　例えば、前記担体の前記表面には、さらに、前記第３の領域と前記第５の領域との間に配置された第６の領域が形成され、前記電気泳動支持体は、さらに、前記第６の領域に配置され、前記試料の第６の等電点に対応する第６のｐＨを形成するための第６の金属酸化膜を備え、前記第６の金属酸化膜は、前記第１の金属酸化物で形成され、前記第６の領域において点在する複数の第３の金属酸化膜片と、前記第２の金属酸化物で形成され、前記第６の領域において点在する複数の第４の金属酸化膜片と、を含み、前記担体を平面視した際に、前記複数の第３の金属酸化膜片の各々は、前記複数の第４の金属酸化膜片の各々と互いに重ならないように配置されており、前記複数の第１の金属酸化膜片と前記複数の第３の金属酸化膜片とは、面積及び数の少なくとも一方が異なり、且つ、前記複数の第２の金属酸化膜片と前記複数の第４の金属酸化膜片とは、面積及び数の少なくとも一方が異なるように構成してもよい。 For example, on the surface of the carrier, a sixth region is further formed, which is disposed between the third region and the fifth region, and the electrophoresis support further includes the sixth. And a sixth metal oxide film for forming a sixth pH corresponding to the sixth isoelectric point of the sample, the sixth metal oxide film being the first metal A plurality of third metal oxide film pieces formed of an oxide and dispersed in the sixth region, and a plurality of fourth formed in the second region and formed of the second metal oxide And each of the plurality of third metal oxide film pieces does not overlap with each of the plurality of fourth metal oxide film pieces when the carrier is viewed in a plan view. And the plurality of first metal oxide film pieces and the plurality of third metal oxide film pieces are And differ by at least one of the number, and said a plurality of second metal oxide film pieces and the plurality of fourth metal oxide film pieces, at least one of the area and the number may be configured differently.
　本態様によれば、ｐＨ勾配のｐＨ階調をより細分化することができる。 According to this aspect, the pH gradation of the pH gradient can be further subdivided.
　例えば、前記第５の金属酸化膜は、前記第１の金属酸化物で形成され、前記第５の領域において点在する複数の第１の金属酸化膜片と、前記第２の金属酸化物で形成され、前記第５の領域において点在する複数の第２の金属酸化膜片と、を含み、前記担体を平面視した際に、前記複数の第１の金属酸化膜片の各々は、前記複数の第２の金属酸化膜片の各々と互いに重なるように配置されているように構成してもよい。 For example, the fifth metal oxide film is formed of the first metal oxide, and includes a plurality of first metal oxide film pieces scattered in the fifth region and the second metal oxide. And a plurality of second metal oxide film pieces formed and scattered in the fifth region, and when the carrier is viewed in plan, each of the plurality of first metal oxide film pieces is It may be configured to be arranged to overlap with each of the plurality of second metal oxide film pieces.
　本態様によれば、担体を平面視した際に、複数の第１の金属酸化膜片の各々は、複数の第２の金属酸化膜片の各々と互いに重なるように配置されているので、第５の領域の面積を十分に確保できない場合であっても、第５の金属酸化膜を第５の領域にコンパクトに配置することができる。 According to this aspect, each of the plurality of first metal oxide film pieces is disposed so as to overlap with each of the plurality of second metal oxide film pieces in plan view of the carrier. Even if the area of the region 5 can not be sufficiently secured, the fifth metal oxide film can be compactly disposed in the fifth region.
　例えば、前記第１の領域、前記第２の領域、前記第３の領域及び前記第４の領域の各区間長のうち少なくとも２つの区間長は、互いに異なるように構成してもよい。 For example, at least two section lengths among the section lengths of the first area, the second area, the third area, and the fourth area may be configured to be different from each other.
　本態様によれば、試料が特定の等電点を比較的多く含む場合に、当該特定の等電点の試料を精度良く分離することができる。 According to this aspect, when the sample contains a relatively large number of specific isoelectric points, it is possible to accurately separate the sample of the specific isoelectric point.
　また、本発明の一態様に係る電気泳動装置は、試料を電気泳動するための電気泳動装置であって、容器と、前記容器の内部に配置された一対の電極と、上述したいずれかの電気泳動支持体と、を備え、前記一対の電極はそれぞれ、前記電気泳動支持体の前記試料の泳動方向における両端部に配置されている。 An electrophoresis apparatus according to an aspect of the present invention is an electrophoresis apparatus for electrophoresis of a sample, comprising: a container; a pair of electrodes disposed inside the container; A migration support, and the pair of electrodes are respectively disposed at both ends of the electrophoresis support in the migration direction of the sample.
　本態様によれば、ｐＨ勾配のｐＨ階調の数を増やした場合であっても、電気泳動支持体の製造プロセスが複雑になるのを抑制することができる。 According to this aspect, even when the number of pH gradations of the pH gradient is increased, the manufacturing process of the electrophoresis support can be suppressed from being complicated.
　なお、これらの包括的又は具体的な態様は、システム、方法、集積回路、コンピュータプログラム又はコンピュータ読み取り可能なＣＤ－ＲＯＭなどの記録媒体で実現されてもよく、システム、方法、集積回路、コンピュータプログラム又は記録媒体の任意な組み合わせで実現されてもよい。 Note that these general or specific aspects may be realized by a system, a method, an integrated circuit, a computer program, or a recording medium such as a computer readable CD-ROM, a system, a method, an integrated circuit, a computer program Or it may be realized by any combination of recording media.
　以下、実施の形態について、図面を参照しながら具体的に説明する。 Embodiments will be specifically described below with reference to the drawings.
　なお、以下で説明する実施の形態は、いずれも包括的又は具体的な例を示すものである。以下の実施の形態で示される数値、形状、材料、構成要素、構成要素の配置位置及び接続形態、ステップ、ステップの順序などは、一例であり、本発明を限定する主旨ではない。また、以下の実施の形態における構成要素のうち、最上位概念を示す独立請求項に記載されていない構成要素については、任意の構成要素として説明される。 Note that all the embodiments described below show general or specific examples. Numerical values, shapes, materials, components, arrangement positions and connection forms of components, steps, order of steps, and the like shown in the following embodiments are merely examples, and the present invention is not limited thereto. Further, among the components in the following embodiments, components not described in the independent claim indicating the highest concept are described as arbitrary components.
　（実施の形態１）
　［１－１．電気泳動装置の構成］
　まず、図１及び図２を参照しながら、実施の形態１に係る電気泳動装置２の構成について説明する。図１は、実施の形態１に係る電気泳動装置２の構成を示す上面図である。図２は、図１のＩＩ－ＩＩ線による、実施の形態１に係る電気泳動装置２の断面図である。 Embodiment 1
[1-1. Configuration of electrophoresis apparatus]
First, the configuration of the electrophoresis device 2 according to the first embodiment will be described with reference to FIGS. 1 and 2. FIG. 1 is a top view showing the configuration of the electrophoresis device 2 according to the first embodiment. FIG. 2 is a cross-sectional view of the electrophoresis device 2 according to the first embodiment, taken along line II-II in FIG.
　図１及び図２に示すように、電気泳動装置２は、試料４の等電点の違いを利用して試料４を分離する等電点電気泳動を行うための装置である。電気泳動装置２は、容器６と、電気泳動支持体８と、一対の電極１０及び１２と、電源装置１４とを備えている。なお、試料４は、例えば互いに異なる複数の等電点をそれぞれ有する複数のタンパク質を含んでいる。 As shown in FIGS. 1 and 2, the electrophoresis apparatus 2 is an apparatus for performing isoelectric focusing in which the sample 4 is separated using the difference in the isoelectric point of the sample 4. The electrophoresis apparatus 2 includes a container 6, an electrophoresis support 8, a pair of electrodes 10 and 12, and a power supply device 14. The sample 4 contains, for example, a plurality of proteins each having a plurality of different isoelectric points.
　容器６は、上面に開口部１６を有している。容器６の内部には液体１８が貯められている。液体１８は、例えばｐＨ７の純水である。容器６の材料は、例えば高分子等の樹脂、シリコン又は金属等で形成されている。 The container 6 has an opening 16 on the top surface. The liquid 18 is stored inside the container 6. The liquid 18 is, for example, pure water of pH 7. The material of the container 6 is made of, for example, a resin such as a polymer, silicon, or a metal.
　電気泳動支持体８は、担体２０と、金属酸化膜体２２とを有している。電気泳動支持体８は、容器６の内部に配置され、容器６の内部の液体１８に浸漬されている。 The electrophoresis support 8 has a carrier 20 and a metal oxide film 22. The electrophoresis support 8 is disposed inside the container 6 and immersed in the liquid 18 inside the container 6.
　担体２０は、例えばセルロースアセテート等の多孔質体で形成されている。担体２０は、Ｘ軸方向に長い短冊状に形成されている。担体２０の長手方向（Ｘ軸方向）における大きさは、例えば３０ｍｍであり、担体２０の短手方向（Ｙ軸方向）における大きさは、例えば２ｍｍである。また、担体２０の厚み方向（Ｚ軸方向）における大きさは、例えば０．１５ｍｍである。なお、試料４は、担体２０の長手方向に沿って、Ｘ軸のマイナス側からプラス側に向かう方向に泳動する。 The carrier 20 is formed of, for example, a porous body such as cellulose acetate. The carrier 20 is formed in a long strip shape in the X-axis direction. The size of the carrier 20 in the longitudinal direction (X-axis direction) is, for example, 30 mm, and the size of the carrier 20 in the lateral direction (Y-axis direction) is, for example, 2 mm. The size of the carrier 20 in the thickness direction (Z-axis direction) is, for example, 0.15 mm. The sample 4 migrates in the direction from the minus side to the plus side of the X axis along the longitudinal direction of the carrier 20.
　金属酸化膜体２２は、担体２０の長手方向に沿ってｐＨ勾配を形成するためのものである。金属酸化膜体２２は、担体２０の上面２４（表面の一例）に配置されている。金属酸化膜体２２は、液体１８に対する酸化還元反応により、例えばｐＨ４～ｐＨ１０の７つのｐＨ階調を有するｐＨ勾配を形成する。金属酸化膜体２２の具体的な構成については後述する。 The metal oxide film 22 is for forming a pH gradient along the longitudinal direction of the carrier 20. The metal oxide film 22 is disposed on the upper surface 24 (an example of the surface) of the carrier 20. The metal oxide film 22 forms a pH gradient having seven pH gradations, for example, pH 4 to pH 10, by the redox reaction to the liquid 18. The specific configuration of the metal oxide film 22 will be described later.
　一対の電極１０及び１２は、容器６の内部に配置され、容器６の内部の液体１８に浸漬されている。一対の電極１０及び１２はそれぞれ、陽極及び陰極として機能し、担体２０の長手方向（試料４の泳動方向）における両端部に接触する位置に配置されている。すなわち、電気泳動支持体８は、一対の電極１０及び１２の間に配置されている。一対の電極１０及び１２の各々の材料は、例えば金、白金、銅、炭素又はこれらの複合体等の導電性材料で形成されている。 The pair of electrodes 10 and 12 are disposed inside the container 6 and immersed in the liquid 18 inside the container 6. The pair of electrodes 10 and 12 function as an anode and a cathode, respectively, and are disposed at positions in contact with both ends in the longitudinal direction (the migration direction of the sample 4) of the carrier 20. That is, the electrophoresis support 8 is disposed between the pair of electrodes 10 and 12. The material of each of the pair of electrodes 10 and 12 is formed of, for example, a conductive material such as gold, platinum, copper, carbon, or a composite thereof.
　なお、本実施の形態では、一対の電極１０及び１２を電気泳動支持体８と容器６の側壁との間に配置したが、これに限定されない。例えば一対の電極１０及び１２を、電気泳動支持体８と容器６の底部との間に配置してもよい。この場合においても、一対の電極１０及び１２はそれぞれ、担体２０の長手方向における両端部に接触する位置に配置される。 In the present embodiment, the pair of electrodes 10 and 12 are disposed between the electrophoresis support 8 and the side wall of the container 6, but the present invention is not limited to this. For example, a pair of electrodes 10 and 12 may be disposed between the electrophoresis support 8 and the bottom of the container 6. Also in this case, the pair of electrodes 10 and 12 are disposed at positions contacting the both ends in the longitudinal direction of the carrier 20, respectively.
　電源装置１４は、容器６の外部に配置され、一対の電極１０及び１２の各々に電気的に接続されている。電源装置１４は、一対の電極１０及び１２の間に所定の直流電圧を印加する。 The power supply 14 is disposed outside the container 6 and electrically connected to each of the pair of electrodes 10 and 12. The power supply device 14 applies a predetermined DC voltage between the pair of electrodes 10 and 12.
　［１－２．金属酸化膜体の構成］
　次に、図３を参照しながら、実施の形態１に係る金属酸化膜体２２の構成について説明する。図３は、実施の形態１に係る電気泳動支持体８の金属酸化膜体２２の構成を示す上面図である。 [1-2. Configuration of metal oxide film]
Next, the configuration of the metal oxide film body 22 according to the first embodiment will be described with reference to FIG. FIG. 3 is a top view showing the configuration of the metal oxide film 22 of the electrophoresis support 8 according to the first embodiment.
　図３に示すように、金属酸化膜体２２は、複数の金属酸化膜２６～３８を有している。複数の金属酸化膜２６～３８はそれぞれ、担体２０の上面２４における複数の領域４０～５２に配置されている。複数の領域４０～５２は、担体２０の長手方向に沿って、この順に一列に並んで配置されている。複数の領域４０～５２の各々は、例えば矩形状の領域であり、同一の区間長（Ｘ軸方向における長さ）を有している。なお、図３では、説明の都合上、複数の領域４０～５２の各々の境界線を実線の矩形状の枠で表している。 As shown in FIG. 3, the metal oxide film body 22 has a plurality of metal oxide films 26 to 38. The plurality of metal oxide films 26 to 38 are respectively disposed in the plurality of regions 40 to 52 on the upper surface 24 of the carrier 20. The plurality of regions 40 to 52 are arranged in line in this order along the longitudinal direction of the carrier 20. Each of the plurality of regions 40 to 52 is, for example, a rectangular region, and has the same section length (length in the X-axis direction). In FIG. 3, for convenience of description, the boundary lines of the plurality of regions 40 to 52 are represented by solid rectangular frames.
　金属酸化膜２６（第１の金属酸化膜の一例）は、金属酸化物であるＴｉＯ２（第１の金属酸化物の一例）で形成されている。金属酸化膜２６は、担体２０の領域４０（第１の領域の一例）の全域に亘って均一に配置されている。金属酸化膜２６は、例えばｐＨ４のｐＨ階調を形成する。このような金属酸化膜２６が領域４０の全域に亘って配置されることにより、領域４０の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ４の等電点（第１の等電点の一例）に対応するｐＨ４（第１のｐＨの一例）となる。 The metal oxide film 26 (an example of a first metal oxide film) is formed of TiO 2 (an example of a first metal oxide) which is a metal oxide. The metal oxide film 26 is uniformly disposed over the entire region 40 (an example of the first region) of the carrier 20. The metal oxide film 26 forms a pH gradation of, for example, pH 4. By arranging such a metal oxide film 26 over the entire region 40, the pH gradation of the liquid 18 in the vicinity of the region 40 is, for example, the isoelectric point of pH 4 of the sample 4 (first isoelectric point PH corresponding to 4) (an example of the first pH).
　なお、金属酸化物の一例であるＴｉＯ２で形成された金属酸化膜では、ｐＨ７の純水中で金属酸化膜の表面に存在するＯＨ基の水素原子（Ｈ）が液中に放出される。これにより、金属酸化膜の表面電位がマイナスになる。液中に放出された水素原子は、液中で水素イオン（Ｈ＋）となるため、金属酸化膜の近傍の溶液は酸性寄り（例えばｐＨ４）になる。このことは、後述する金属酸化膜２８及び３０についても同様である。 In the metal oxide film formed of TiO 2 which is an example of the metal oxide, hydrogen atoms (H) of OH groups present on the surface of the metal oxide film in pure water of pH 7 are released into the liquid. Thereby, the surface potential of the metal oxide film becomes negative. The hydrogen atoms released into the solution become hydrogen ions (H + ) in the solution, so the solution in the vicinity of the metal oxide film becomes acidic (for example, pH 4). The same applies to metal oxide films 28 and 30 described later.
　金属酸化膜２８（第２の金属酸化膜の一例）は、金属酸化膜２６と同様に、ＴｉＯ２で形成されている。金属酸化膜２８は、担体２０の領域４２（第２の領域の一例）において格子状に点在された複数の金属酸化膜片５４を有している。すなわち、金属酸化膜２８は、複数の金属酸化膜片５４の集合体である。複数の金属酸化膜片５４の各々は、平面視で円形状に形成されている。すなわち、担体２０を平面視した際に、担体２０の領域４２において金属酸化膜２８が占める面積割合（第２の面積割合の一例）は、担体２０の領域４０において金属酸化膜２６が占める面積割合（第１の面積割合の一例）よりも小さい。金属酸化膜２８は、例えばｐＨ４のｐＨ階調を形成する。領域４２は、担体２０が露出している部分と、金属酸化膜２８とにより形成される。金属酸化膜２８は、領域４２におけるｐＨ階調に影響を及ぼす。そのため、領域４２のｐＨ階調は、金属酸化膜２８が形成するｐＨ階調（例えばｐＨ４）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域４２の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ５の等電点（第２の等電点の一例）に対応するｐＨ５（第２のｐＨの一例）となる。 The metal oxide film 28 (an example of the second metal oxide film) is formed of TiO 2 similarly to the metal oxide film 26. The metal oxide film 28 has a plurality of metal oxide film pieces 54 scattered in a lattice shape in the region 42 (an example of the second region) of the carrier 20. That is, the metal oxide film 28 is an aggregate of a plurality of metal oxide film pieces 54. Each of the plurality of metal oxide film pieces 54 is formed in a circular shape in plan view. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 28 in the region 42 of the carrier 20 (an example of the second area ratio) is the area ratio occupied by the metal oxide film 26 in the region 40 of the carrier 20 (An example of the first area ratio) is smaller. The metal oxide film 28 forms a pH gradation of, for example, pH 4. The region 42 is formed by the exposed portion of the carrier 20 and the metal oxide film 28. The metal oxide film 28 affects the pH gradation in the region 42. Therefore, the pH gradation of the region 42 is formed by a combination of the pH gradation (for example, pH 4) formed by the metal oxide film 28 and the pH gradation (for example, pH 7) formed by the pure water. Thus, the pH gradation of the liquid 18 in the vicinity of the region 42 becomes, for example, pH 5 (an example of a second pH) corresponding to the isoelectric point (an example of a second isoelectric point) of pH 5 of the sample 4.
　なお、純水のｐＨ７よりも低いｐＨ階調では、上記の面積割合が小さくなるほど、液体１８と接触するＴｉＯ２の表面積が小さくなるため、ＴｉＯ２の表面に存在するＯＨ基から液体１８中に放出される水素イオン（Ｈ＋）の量が少なくなる。そのため、領域４２により形成されるｐＨ階調は、領域４０により形成されるｐＨ階調よりも高くなる。 At a pH gradation lower than pH 7 of pure water, the surface area of TiO 2 in contact with liquid 18 decreases as the area ratio decreases, so OH groups present on the surface of TiO 2 enter into liquid 18 The amount of hydrogen ions (H + ) released is reduced. Therefore, the pH gradation formed by the region 42 is higher than the pH gradation formed by the region 40.
　金属酸化膜３０は、金属酸化膜２６と同様に、ＴｉＯ２で形成されている。金属酸化膜３０は、担体２０の領域４４において格子状に点在された複数の金属酸化膜片５６を有している。すなわち、金属酸化膜３０は、複数の金属酸化膜片５６の集合体である。複数の金属酸化膜片５６の各々は、平面視で円形状に形成されている。複数の金属酸化膜片５６の数は、複数の金属酸化膜片５４の数と同一である。また、複数の金属酸化膜片５６の各々の直径は、複数の金属酸化膜片５４の各々の直径よりも小さい。すなわち、担体２０を平面視した際に、担体２０の領域４４において金属酸化膜３０が占める面積割合は、担体２０の領域４２において金属酸化膜２８が占める面積割合よりも小さい。金属酸化膜３０は、例えばｐＨ４のｐＨ階調を形成する。領域４４は、担体２０が露出している部分と、金属酸化膜３０とにより形成される。金属酸化膜３０は、領域４４におけるｐＨ階調に影響を及ぼす。そのため、領域４４のｐＨ階調は、金属酸化膜３０が形成するｐＨ階調（例えばｐＨ４）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域４４の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ６の等電点に対応するｐＨ６となる。 The metal oxide film 30 is formed of TiO 2 similarly to the metal oxide film 26. The metal oxide film 30 has a plurality of metal oxide film pieces 56 interspersed in a lattice shape in the region 44 of the carrier 20. That is, the metal oxide film 30 is an aggregate of a plurality of metal oxide film pieces 56. Each of the plurality of metal oxide film pieces 56 is formed in a circular shape in plan view. The number of the plurality of metal oxide film pieces 56 is the same as the number of the plurality of metal oxide film pieces 54. Also, the diameter of each of the plurality of metal oxide film pieces 56 is smaller than the diameter of each of the plurality of metal oxide film pieces 54. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 30 in the region 44 of the carrier 20 is smaller than the area ratio occupied by the metal oxide film 28 in the region 42 of the carrier 20. The metal oxide film 30 forms, for example, a pH gradation of pH4. The region 44 is formed by the exposed portion of the carrier 20 and the metal oxide film 30. The metal oxide film 30 affects the pH gradation in the region 44. Therefore, the pH gradation of the region 44 is formed by a combination of the pH gradation (for example, pH 4) formed by the metal oxide film 30 and the pH gradation (for example, pH 7) formed by the pure water. Thereby, the pH gradation of the liquid 18 in the vicinity of the region 44 becomes, for example, pH 6 corresponding to the isoelectric point of pH 6 of the sample 4.
　なお、上述したように、純水のｐＨ７よりも低いｐＨ階調では、上記の面積割合が小さくなるほど、液体１８と接触するＴｉＯ２の表面積が小さくなるため、ＴｉＯ２の表面に存在するＯＨ基から液体１８中に放出される水素イオン（Ｈ＋）の量が少なくなる。そのため、領域４４により形成されるｐＨ階調は、領域４２により形成されるｐＨ階調よりも高くなる。 As described above, when the pH gradation is lower than pH 7 of pure water, the surface area of TiO 2 in contact with liquid 18 decreases as the area ratio decreases, so the OH groups present on the surface of TiO 2 The amount of hydrogen ions (H + ) released into the liquid 18 from the Therefore, the pH gradation formed by the region 44 is higher than the pH gradation formed by the region 42.
　金属酸化膜３２は、金属酸化物であるＮｉＯで形成されている。金属酸化膜３２は、担体２０の領域４６の全域に亘って均一に配置されている。金属酸化膜３２は、例えばｐＨ７のｐＨ階調を形成する。このような金属酸化膜３２が領域４６の全域に亘って配置されることにより、領域４６の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ７の等電点に対応するｐＨ７となる。 The metal oxide film 32 is formed of NiO which is a metal oxide. The metal oxide film 32 is uniformly disposed over the entire region 46 of the carrier 20. The metal oxide film 32 forms a pH gradation of, for example, pH 7. By arranging such a metal oxide film 32 over the entire region 46, the pH gradation of the liquid 18 in the vicinity of the region 46 becomes, for example, pH 7 corresponding to the isoelectric point of pH 7 of the sample 4.
　なお、金属酸化物の一例であるＮｉＯで形成された金属酸化膜では、ｐＨ７の純水中で金属酸化膜の表面に存在するＯＨ基が液中の水素イオン（Ｈ＋）を受け取る。これにより、金属酸化膜の表面電位がプラスになる。液中では水素イオン（Ｈ＋）が減少するため、金属酸化膜の近傍の溶液は中性寄り（例えばｐＨ７）になる。 In a metal oxide film formed of NiO, which is an example of a metal oxide, OH groups present on the surface of the metal oxide film in pure water of pH 7 receive hydrogen ions (H + ) in the liquid. Thereby, the surface potential of the metal oxide film becomes positive. Since hydrogen ions (H + ) decrease in the solution, the solution in the vicinity of the metal oxide film becomes neutral (eg, pH 7).
　金属酸化膜３４は、金属酸化物であるＭｇＯ（第２の金属酸化物の一例）で形成されている。金属酸化膜３４は、担体２０の領域４８において格子状に点在された複数の金属酸化膜片５８を有している。すなわち、金属酸化膜３４は、複数の金属酸化膜片５８の集合体である。複数の金属酸化膜片５８の各々は、平面視で円形状に形成されている。金属酸化膜３４は、例えばｐＨ１０のｐＨ階調を形成する。領域４８は、担体２０が露出している部分と、金属酸化膜３４とにより形成される。金属酸化膜３４は、領域４８におけるｐＨ階調に影響を及ぼす。そのため、領域４８のｐＨ階調は、金属酸化膜３４が形成するｐＨ階調（例えばｐＨ１０）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域４８の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ８の等電点に対応するｐＨ８となる。 The metal oxide film 34 is formed of MgO (an example of a second metal oxide) which is a metal oxide. The metal oxide film 34 has a plurality of metal oxide film pieces 58 interspersed in a lattice shape in the region 48 of the carrier 20. That is, the metal oxide film 34 is an aggregate of a plurality of metal oxide film pieces 58. Each of the plurality of metal oxide film pieces 58 is formed in a circular shape in plan view. The metal oxide film 34 forms a pH gradation of, for example, pH 10. The region 48 is formed by the exposed portion of the carrier 20 and the metal oxide film 34. The metal oxide film 34 affects the pH gradation in the region 48. Therefore, the pH gradation of the region 48 is formed by a combination of pH gradation (for example, pH 10) formed by the metal oxide film 34 and pH gradation (for example, pH 7) formed by pure water. Thus, the pH gradation of the liquid 18 in the vicinity of the region 48 is, for example, pH 8 corresponding to the isoelectric point of pH 8 of the sample 4.
　なお、金属酸化物の一例であるＭｇＯで形成された金属酸化膜では、ｐＨ７の純水中で金属酸化膜の表面に存在するＯＨ基が液中の水素イオン（Ｈ＋）を受け取る。これにより、金属酸化膜の表面電位がプラスになる。液中では水素イオン（Ｈ＋）が減少するため、金属酸化膜の近傍の溶液はアルカリ性寄り（例えばｐＨ１０）になる。このことは、後述する金属酸化膜３６及び３８についても同様である。 In a metal oxide film formed of MgO, which is an example of a metal oxide, OH groups present on the surface of the metal oxide film in pure water of pH 7 receive hydrogen ions (H + ) in the liquid. Thereby, the surface potential of the metal oxide film becomes positive. Since hydrogen ions (H + ) decrease in the liquid, the solution in the vicinity of the metal oxide film becomes more alkaline (for example, pH 10). The same applies to metal oxide films 36 and 38 described later.
　金属酸化膜３６（第３の金属酸化膜の一例）は、金属酸化膜３４と同様に、ＭｇＯで形成されている。金属酸化膜３６は、担体２０の領域５０（第３の領域の一例）において格子状に点在された複数の金属酸化膜片６０を有している。すなわち、金属酸化膜３６は、複数の金属酸化膜片６０の集合体である。複数の金属酸化膜片６０の各々は、平面視で円形状に形成されている。複数の金属酸化膜片６０の数は、複数の金属酸化膜片５８の数と同一である。また、複数の金属酸化膜片６０の各々の直径は、複数の金属酸化膜片５８の各々の直径よりも大きい。すなわち、担体２０を平面視した際に、担体２０の領域５０において金属酸化膜３６が占める面積割合は、担体２０の領域４８において金属酸化膜３４が占める面積割合よりも大きい。金属酸化膜３６は、例えばｐＨ１０のｐＨ階調を形成する。領域５０は、担体２０が露出している部分と、金属酸化膜３６とにより形成される。金属酸化膜３６は、領域５０におけるｐＨ階調に影響を及ぼす。そのため、領域５０のｐＨ階調は、金属酸化膜３６が形成するｐＨ階調（例えばｐＨ１０）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域５０の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ９の等電点（第３の等電点の一例）に対応するｐＨ９（第３のｐＨの一例）となる。 The metal oxide film 36 (an example of the third metal oxide film) is formed of MgO, similarly to the metal oxide film 34. The metal oxide film 36 has a plurality of metal oxide film pieces 60 interspersed in a lattice shape in a region 50 (an example of a third region) of the carrier 20. That is, the metal oxide film 36 is an aggregate of a plurality of metal oxide film pieces 60. Each of the plurality of metal oxide film pieces 60 is formed in a circular shape in plan view. The number of the plurality of metal oxide film pieces 60 is the same as the number of the plurality of metal oxide film pieces 58. In addition, the diameter of each of the plurality of metal oxide film pieces 60 is larger than the diameter of each of the plurality of metal oxide film pieces 58. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 36 in the region 50 of the carrier 20 is larger than the area ratio occupied by the metal oxide film 34 in the region 48 of the carrier 20. The metal oxide film 36 forms, for example, a pH gradation of pH 10. Region 50 is formed of a portion where carrier 20 is exposed and metal oxide film 36. The metal oxide film 36 affects the pH gradation in the region 50. Therefore, the pH gradation of the region 50 is formed by a combination of pH gradation (for example, pH 10) formed by the metal oxide film 36 and pH gradation (for example, pH 7) formed by pure water. Thus, the pH gradation of the liquid 18 in the vicinity of the region 50 is, for example, pH 9 (an example of a third pH) corresponding to the isoelectric point (an example of a third isoelectric point) of pH 9 of the sample 4.
　なお、純水のｐＨ７よりも高いｐＨ階調では、上記の面積割合が小さくなるほど、液体１８と接触するＭｇＯの表面積が小さくなるため、ＭｇＯの表面に存在するＯＨ基が液体１８中の水素イオン（Ｈ＋）を受け取る量が少なくなる。そのため、領域５０により形成されるｐＨ階調は、領域４８により形成されるｐＨ階調よりも高くなる。 At a pH gradation higher than pH 7 of pure water, the surface area of MgO in contact with the liquid 18 decreases as the above area ratio decreases, so the OH groups present on the surface of MgO are hydrogen ions in the liquid 18 The amount of receiving (H + ) decreases. Therefore, the pH gradation formed by the region 50 is higher than the pH gradation formed by the region 48.
　金属酸化膜３８（第４の金属酸化膜の一例）は、金属酸化膜３４と同様に、ＭｇＯで形成されている。金属酸化膜３８は、担体２０の領域５２（第４の領域の一例）の全域に亘って均一に配置されている。すなわち、担体２０を平面視した際に、担体２０の領域５２において金属酸化膜３８が占める面積割合（第４の面積割合の一例）は、担体２０の領域５０において金属酸化膜３６が占める面積割合（第３の面積割合の一例）よりも大きい。金属酸化膜３８は、例えばｐＨ１０のｐＨ階調を形成する。このような金属酸化膜３８が領域５２の全域に亘って配置されることにより、領域５２の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ１０の等電点（第４の等電点の一例）に対応するｐＨ１０（第４のｐＨの一例）となる。 The metal oxide film 38 (an example of the fourth metal oxide film) is formed of MgO, similarly to the metal oxide film 34. The metal oxide film 38 is uniformly disposed over the entire region 52 (an example of a fourth region) of the carrier 20. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 38 in the region 52 of the carrier 20 (an example of the fourth area ratio) is the area ratio occupied by the metal oxide film 36 in the region 50 of the carrier 20 (An example of the third area ratio) is larger. The metal oxide film 38 forms, for example, a pH gradation of pH 10. By arranging such a metal oxide film 38 over the entire region 52, the pH gradation of the liquid 18 in the vicinity of the region 52 is, for example, the isoelectric point of pH 10 of the sample 4 (the fourth isoelectric point PH corresponding to 10) (an example of a fourth pH).
　なお、上述したように、純水のｐＨ７よりも高いｐＨ階調では、上記の面積割合が小さくなるほど、液体１８と接触するＭｇＯの表面積が小さくなるため、ＭｇＯの表面に存在するＯＨ基が液体１８中の水素イオン（Ｈ＋）を受け取る量が少なくなる。そのため、領域５２により形成されるｐＨ階調は、領域５０により形成されるｐＨ階調よりも高くなる。 As described above, at a pH gradation higher than pH 7 of pure water, the surface area of MgO in contact with the liquid 18 decreases as the area ratio decreases, so the OH groups present on the surface of MgO are liquid The amount of hydrogen ions (H + ) in 18 is reduced. Therefore, the pH gradation formed by the area 52 is higher than the pH gradation formed by the area 50.
　以上のように、複数の金属酸化膜２６～３８は、電極１２から電極１０に向かって、ｐＨ４～ｐＨ１０の７つのｐＨ階調が昇順に並ぶように配置されている。これにより、電気泳動支持体８には、担体２０の長手方向に沿って、ｐＨ４～ｐＨ１０の７つのｐＨ階調がこの順に一列に並んだｐＨ勾配が形成される。 As described above, the plurality of metal oxide films 26 to 38 are arranged such that seven pH gradations of pH 4 to pH 10 are arranged in ascending order from the electrode 12 to the electrode 10. As a result, a pH gradient is formed on the electrophoresis support 8 along the longitudinal direction of the carrier 20, in which seven pH gradations of pH 4 to pH 10 are aligned in this order.
　なお、上述した複数の金属酸化膜２６～３８の各々は、例えばＡＬＤ（Ａｔｏｍｉｃ　Ｌａｙｅｒ　Ｄｅｐｏｓｉｔｉｏｎ：原子層堆積）法を用いて成膜される。このとき、担体２０の上面２４を例えばメタルマスク等で覆うことにより、複数の金属酸化膜２６～３８の各々を所望の形状に形成することができる。複数の金属酸化膜２６～３８はそれぞれ、担体２０の上面２４における複数の領域４０～５２に堆積されるだけでなく、複数の領域４０～５２にそれぞれ対応する担体２０の内部にも浸透される。 Each of the plurality of metal oxide films 26 to 38 described above is formed, for example, by using an ALD (Atomic Layer Deposition) method. At this time, by covering the upper surface 24 of the carrier 20 with, for example, a metal mask or the like, each of the plurality of metal oxide films 26 to 38 can be formed into a desired shape. The plurality of metal oxide films 26 to 38 are not only deposited on the plurality of regions 40 to 52 on the upper surface 24 of the carrier 20 but also penetrate the inside of the carrier 20 corresponding to the plurality of regions 40 to 52 respectively. .
　［１－３．電気泳動装置の動作］
　次に、図１～図３を参照しながら、実施の形態１に係る電気泳動装置２の動作について説明する。 [1-3. Operation of electrophoresis apparatus]
Next, the operation of the electrophoresis device 2 according to the first embodiment will be described with reference to FIGS. 1 to 3.
　まず、図１及び図２に示すように、容器６の内部に電気泳動支持体８を配置し、容器６の内部に液体１８を注入する。次に、担体２０の長手方向における一端部（電極１２側の端部）に試料４を注入する。 First, as shown in FIGS. 1 and 2, the electrophoresis support 8 is disposed inside the container 6, and the liquid 18 is injected into the inside of the container 6. Next, the sample 4 is injected into one end (end on the electrode 12 side) in the longitudinal direction of the carrier 20.
　その後、電源装置１４により一対の電極１０及び１２の間に所定の直流電圧を印加する。例えば、一対の電極１０及び１２の間に５００Ｖの直流電圧を１分間印加した後に、１時間半かけて３５００Ｖまで電圧値を上昇させる。さらにその後、一対の電極１０及び１２の間に３５００Ｖの直流電圧を６時間半印加する。これにより、一対の電極１０及び１２の間に電場が形成され、試料４は、この電場の作用を受けて担体２０の内部を移動（泳動）する。 Thereafter, a predetermined DC voltage is applied between the pair of electrodes 10 and 12 by the power supply device 14. For example, after applying a DC voltage of 500 V between the pair of electrodes 10 and 12 for one minute, the voltage value is raised to 3500 V in one and a half hours. Thereafter, a DC voltage of 3500 V is applied for six and a half hours between the pair of electrodes 10 and 12. Thereby, an electric field is formed between the pair of electrodes 10 and 12, and the sample 4 moves (migrates) inside the carrier 20 under the action of the electric field.
　このとき、試料４の移動距離は、試料４の等電点によって異なる。図３に示すように、例えば試料４に等電点がｐＨ４であるタンパク質４ａが含まれる場合には、タンパク質４ａは、ｐＨ４のｐＨ階調を形成する領域４０の位置まで移動する。一方、試料４に等電点がｐＨ５であるタンパク質４ｂが含まれる場合には、タンパク質４ｂは、ｐＨ５のｐＨ階調を形成する領域４２の位置まで移動する。これにより、試料４に含まれる複数のタンパク質の各々は、等電点の違いによって複数の領域４０～５２のいずれかの位置に分離される。 At this time, the movement distance of the sample 4 differs depending on the isoelectric point of the sample 4. As shown in FIG. 3, for example, when the sample 4 contains the protein 4 a having an isoelectric point of pH 4, the protein 4 a moves to the position of the region 40 forming a pH gradation of pH 4. On the other hand, when the sample 4 contains the protein 4b having an isoelectric point of pH 5, the protein 4b moves to the position of the region 42 forming a pH gradation of pH5. Thereby, each of the plurality of proteins contained in the sample 4 is separated into any one of the plurality of regions 40 to 52 due to the difference in isoelectric point.
　試料４を分離した後に、電気泳動支持体８を染色することにより、分離された試料４の位置を検出することができる。電気泳動支持体８の染色として、例えば銀染色等が用いられる。 After separating the sample 4, the position of the separated sample 4 can be detected by staining the electrophoresis support 8. As a stain of the electrophoresis support 8, for example, silver stain or the like is used.
　なお、電気泳動を行う前に、蛍光色素を用いて試料４を染色してもよい。この場合、電気泳動を行った後の電気泳動支持体８に対して励起光を照射し、蛍光を観察することにより、分離された試料４の位置を検出することができる。 Note that, before performing electrophoresis, the sample 4 may be stained using a fluorescent dye. In this case, the position of the separated sample 4 can be detected by irradiating the electrophoresis support 8 after the electrophoresis with excitation light and observing the fluorescence.
　あるいは、試料４を分離した後に、電気泳動支持体８に紫外線又は近赤外線等の光を照射し、照射した光の透過光又は反射光を検出してもよい。タンパク質を含む試料４は、特定の波長の光を吸収する性質を有している。そのため、電気泳動支持体８に照射した光を検出する場合、試料４が位置する場所では、他の場所に比べて検出される光の強度が弱くなる。このような方法によっても、分離された試料４の位置を検出することができる。 Alternatively, after the sample 4 is separated, the electrophoresis support 8 may be irradiated with light such as ultraviolet light or near infrared light, and the transmitted light or the reflected light of the irradiated light may be detected. The sample 4 containing a protein has the property of absorbing light of a specific wavelength. Therefore, in the case where the light irradiated to the electrophoresis support 8 is detected, the intensity of the detected light is weaker at the place where the sample 4 is located than at other places. The position of the separated sample 4 can also be detected by such a method.
　［１－４．効果］
　本実施の形態では、担体２０の領域４０において金属酸化膜２６が占める面積割合と、担体２０の領域４２において金属酸化膜２８が占める面積割合と、担体２０の領域４４において金属酸化膜３０が占める面積割合とを互いに異ならせている。これにより、例えばｐＨ４～ｐＨ６の３つのｐＨ階調を形成する場合には、ｐＨ階調の数よりも少ない１種類の金属酸化物ＴｉＯ２を用いて３つの金属酸化膜２６，２８及び３０を成膜することができる。 [1-4. effect]
In the present embodiment, the area ratio occupied by the metal oxide film 26 in the region 40 of the carrier 20, the area ratio occupied by the metal oxide film 28 in the region 42 of the carrier 20, and the metal oxide film 30 occupied in the region 44 of the carrier 20 The area ratio is made different from each other. Thus, for example, in the case of forming three pH gradations of pH 4 to pH 6, one metal oxide TiO 2 which is smaller than the number of pH gradations is used to form three metal oxide films 26, 28 and 30. A film can be formed.
　同様に、本実施の形態では、担体２０の領域４８において金属酸化膜３４が占める面積割合と、担体２０の領域５０において金属酸化膜３６が占める面積割合と、担体２０の領域５２において金属酸化膜３８が占める面積割合とを互いに異ならせている。これにより、例えばｐＨ８～ｐＨ１０の３つのｐＨ階調を形成する場合には、ｐＨ階調の数よりも少ない１種類の金属酸化物ＭｇＯを用いて３つの金属酸化膜３４，３６及び３８を成膜することができる。 Similarly, in the present embodiment, the area ratio occupied by the metal oxide film 34 in the region 48 of the carrier 20, the area ratio occupied by the metal oxide film 36 in the region 50 of the carrier 20, and the metal oxide film in the region 52 of the carrier 20 The area ratio occupied by 38 is different from one another. Thus, for example, in the case of forming three pH gradations of pH 8 to pH 10, three metal oxide films 34, 36 and 38 are formed using one kind of metal oxide MgO smaller than the number of pH gradations. It can be membrane.
　したがって、例えばｐＨ４～ｐＨ１０の７つのｐＨ階調を有するｐＨ勾配を形成する場合には、ｐＨ階調の数よりも少ない３種類の金属酸化物ＴｉＯ２、ＮｉＯ及びＭｇＯを用いて７つの金属酸化膜２６～３８を成膜することができる。これにより、ｐＨ勾配のｐＨ階調の数を増やした場合であっても、複数の金属酸化膜２６～３８の成膜工数が増大するのを抑制することができるとともに、複数の金属酸化膜２６～３８の成膜制御を簡素化することができる。その結果、電気泳動支持体８の製造プロセスが複雑になるのを抑制することができる。 Thus, for example, in the case of forming a pH gradient having seven pH gradations, for example, pH 4 to pH 10, oxidation of seven metals using three kinds of metal oxides TiO 2 , NiO and MgO smaller than the number of pH gradations. The films 26 to 38 can be formed. As a result, even when the number of pH gradations of the pH gradient is increased, an increase in the number of steps for forming the plurality of metal oxide films 26 to 38 can be suppressed, and the plurality of metal oxide films 26 can be suppressed. It is possible to simplify the film formation control of ~ 38. As a result, the manufacturing process of the electrophoretic support 8 can be suppressed from being complicated.
　（実施の形態２）
　［２－１．金属酸化膜体の構成］
　次に、図４を参照しながら、実施の形態２に係る電気泳動支持体８Ａの金属酸化膜体２２Ａの構成について説明する。図４は、実施の形態２に係る電気泳動支持体８Ａの金属酸化膜体２２Ａの構成を示す上面図である。なお、以下の各実施の形態において、上記実施の形態１と同一の構成要素には同一の符号を付して、その説明を省略する。 Second Embodiment
[2-1. Configuration of metal oxide film]
Next, the configuration of the metal oxide film 22A of the electrophoresis support 8A according to the second embodiment will be described with reference to FIG. FIG. 4 is a top view showing the configuration of the metal oxide film 22A of the electrophoresis support 8A according to the second embodiment. In the following embodiments, the same components as those in the first embodiment are denoted by the same reference numerals, and the description thereof is omitted.
　図４に示すように、実施の形態２に係る電気泳動支持体８Ａでは、金属酸化膜体２２Ａの構成が上記実施の形態１と異なっている。具体的には、担体２０の領域４０の区間長Ｘ１は、担体２０の領域４２Ａの区間長Ｘ２よりも長く、且つ、担体２０の領域４４Ａの区間長Ｘ３よりも短い。また、担体２０の領域５２の区間長Ｘ１は、担体２０の領域５０Ａの区間長Ｘ２よりも長く、且つ、担体２０の領域４８Ａの区間長Ｘ３よりも短い。なお、担体２０の領域４０，４６及び５２の各区間長Ｘ１は、同一である。 As shown in FIG. 4, in the electrophoresis support 8A according to the second embodiment, the configuration of the metal oxide film 22A is different from that of the first embodiment. Specifically, the section length X1 of the area 40 of the carrier 20 is longer than the section length X2 of the area 42A of the carrier 20 and shorter than the section length X3 of the area 44A of the carrier 20. The section length X1 of the area 52 of the carrier 20 is longer than the section length X2 of the area 50A of the carrier 20 and shorter than the section length X3 of the area 48A of the carrier 20. The section lengths X1 of the regions 40, 46 and 52 of the carrier 20 are the same.
　金属酸化膜３０Ａの複数の金属酸化膜片５６の数は、金属酸化膜２８Ａの複数の金属酸化膜片５４の数よりも多い。また、複数の金属酸化膜片５６の各々の直径は、複数の金属酸化膜片５４の各々の直径よりも小さい。すなわち、担体２０を平面視した際に、担体２０の領域４４Ａにおいて金属酸化膜３０Ａが占める面積割合は、担体２０の領域４２Ａにおいて金属酸化膜２８Ａが占める面積割合よりも小さい。 The number of the plurality of metal oxide film pieces 56 of the metal oxide film 30A is larger than the number of the plurality of metal oxide film pieces 54 of the metal oxide film 28A. Also, the diameter of each of the plurality of metal oxide film pieces 56 is smaller than the diameter of each of the plurality of metal oxide film pieces 54. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 30A in the region 44A of the carrier 20 is smaller than the area ratio occupied by the metal oxide film 28A in the region 42A of the carrier 20.
　金属酸化膜３４Ａの複数の金属酸化膜片５８の数は、金属酸化膜３６Ａの複数の金属酸化膜片６０の数よりも多い。また、複数の金属酸化膜片５８の各々の直径は、複数の金属酸化膜片６０の各々の直径よりも小さい。すなわち、担体２０を平面視した際に、担体２０の領域４８Ａにおいて金属酸化膜３４Ａが占める面積割合は、担体２０の領域５０Ａにおいて金属酸化膜３６Ａが占める面積割合よりも小さい。 The number of the plurality of metal oxide film pieces 58 of the metal oxide film 34A is larger than the number of the plurality of metal oxide film pieces 60 of the metal oxide film 36A. In addition, the diameter of each of the plurality of metal oxide film pieces 58 is smaller than the diameter of each of the plurality of metal oxide film pieces 60. That is, when the carrier 20 is viewed in plan, the area ratio occupied by the metal oxide film 34A in the region 48A of the carrier 20 is smaller than the area ratio occupied by the metal oxide film 36A in the region 50A of the carrier 20.
　［２－２．効果］
　例えば、試料４に等電点がｐＨ６及びｐＨ８であるタンパク質が比較的多く含まれている場合には、上述したように担体２０の領域４４Ａ及び４８Ａの各区間長Ｘ３を区間長Ｘ１及びＸ２よりも長くする。これにより、等電点がｐＨ６及びｐＨ８であるタンパク質をそれぞれ、金属酸化膜３０Ａ及び３４Ａにより精度良く分離することができる。 [2-2. effect]
For example, when the sample 4 contains a relatively large amount of protein having an isoelectric point of pH 6 and pH 8, as described above, each section length X3 of the regions 44A and 48A of the carrier 20 is greater than the section lengths X1 and X2 Also make it longer. Thus, proteins having isoelectric points of pH 6 and pH 8 can be separated with high precision by the metal oxide films 30A and 34A, respectively.
　同様に、例えば試料４に等電点がｐＨ５及びｐＨ９であるタンパク質が比較的多く含まれている場合には、上述したように担体２０の領域４２Ａ及び５０Ａの各区間長Ｘ２を、区間長Ｘ１よりも長くすればよい。これにより、等電点がｐＨ５及びｐＨ９であるタンパク質をそれぞれ、金属酸化膜２８Ａ及び３６Ａにより精度良く分離することができる。 Similarly, for example, when the sample 4 contains a relatively large amount of protein having an isoelectric point of pH 5 and pH 9, as described above, each section length X2 of the regions 42A and 50A of the carrier 20 is set to section length X1. It should be longer than this. As a result, proteins having an isoelectric point of pH 5 and pH 9 can be separated with high precision by the metal oxide films 28A and 36A, respectively.
　同様に、例えば試料４に等電点がｐＨ４及びｐＨ１０であるタンパク質が比較的多く含まれている場合には、上述したように担体２０の領域４０及び５２の各区間長Ｘ１を、区間長Ｘ２及びＸ３よりも長くすればよい。これにより、等電点がｐＨ４及びｐＨ１０であるタンパク質をそれぞれ、金属酸化膜２６及び３８により精度良く分離することができる。 Similarly, for example, when the sample 4 contains a relatively large amount of protein having an isoelectric point of pH 4 and pH 10, as described above, each section length X1 of the regions 40 and 52 of the carrier 20 is set to section length X2 And X3. Thereby, proteins having an isoelectric point of pH 4 and pH 10 can be separated with high precision by the metal oxide films 26 and 38, respectively.
　（実施の形態３）
　［３－１．金属酸化膜体の構成］
　次に、図５を参照しながら、実施の形態３に係る電気泳動支持体８Ｂの金属酸化膜体２２Ｂの構成について説明する。図５は、実施の形態３に係る電気泳動支持体８Ｂの金属酸化膜体２２Ｂの構成を示す上面図である。 Third Embodiment
3-1. Configuration of metal oxide film]
Next, the configuration of the metal oxide film 22B of the electrophoresis support 8B according to the third embodiment will be described with reference to FIG. FIG. 5 is a top view showing the configuration of the metal oxide film 22B of the electrophoresis support 8B according to the third embodiment.
　図５に示すように、実施の形態３に係る電気泳動支持体８Ｂでは、金属酸化膜体２２Ｂの構成が上記実施の形態１と異なっている。具体的には、金属酸化膜体２２Ｂは、複数の金属酸化膜２６～３８に加えて、金属酸化膜６２（第５の金属酸化膜の一例）及び金属酸化膜６４（第６の金属酸化膜の一例）を有している。また、担体２０の領域４２と領域４４との間には領域６６（第５の領域の一例）が配置され、担体２０の領域４８と領域５０との間には領域６８（第６の領域の一例）が配置されている。 As shown in FIG. 5, in the electrophoresis support 8B according to the third embodiment, the configuration of the metal oxide film 22B is different from that of the first embodiment. Specifically, metal oxide film body 22B includes metal oxide film 62 (an example of a fifth metal oxide film) and metal oxide film 64 (sixth metal oxide film) in addition to the plurality of metal oxide films 26 to 38. An example). In addition, a region 66 (an example of a fifth region) is disposed between the region 42 of the carrier 20 and the region 44, and a region 68 (of the sixth region) is disposed between the region 48 and the region 50 of the carrier 20. An example is arranged.
　金属酸化膜６２は、担体２０の領域６６において格子状に点在された複数の金属酸化膜片７０（第１の金属酸化膜片の一例）及び複数の金属酸化膜片７２（第２の金属酸化膜片の一例）を有している。担体２０を平面視した際に、複数の金属酸化膜片７０の各々は、複数の金属酸化膜片７２の各々と互いに重ならないように配置されている。 The metal oxide film 62 includes a plurality of metal oxide film pieces 70 (an example of a first metal oxide film piece) and a plurality of metal oxide film pieces 72 (a second metal) which are scattered in a lattice shape in the region 66 of the carrier 20. (An example of an oxide film piece). When the carrier 20 is viewed in plan, each of the plurality of metal oxide film pieces 70 is arranged so as not to overlap with each of the plurality of metal oxide film pieces 72.
　複数の金属酸化膜片７０の各々はＴｉＯ２で形成され、複数の金属酸化膜片７２の各々はＭｇＯで形成されている。すなわち、金属酸化膜６２は、異なる２種類の金属酸化物で形成されている。複数の金属酸化膜片７０及び７２の各々は、平面視で円形状に形成されている。複数の金属酸化膜片７０の各々の数は、複数の金属酸化膜片７２の各々の数よりも多い。また、複数の金属酸化膜片７０の各々の直径は、複数の金属酸化膜片７２の各々の直径よりも大きい。なお、複数の金属酸化膜片７０の各々の直径は、複数の金属酸化膜片５４の各々の直径と同一である。領域６６は、担体２０が露出している部分と、複数の金属酸化膜片７０と、複数の金属酸化膜片７２とにより形成される。金属酸化膜６２は、領域６６におけるｐＨ階調に影響を及ぼす。そのため、領域６６のｐＨ階調は、複数の金属酸化膜片７０が形成するｐＨ階調（例えばｐＨ４）と、複数の金属酸化膜片７２が形成するｐＨ階調（例えばｐＨ７）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域６６の近傍における液体１８（図１参照）のｐＨ階調は、試料４のｐＨ５．５の等電点（第５の等電点の一例）に対応するｐＨ５．５（第５のｐＨの一例）となる。 Each of the plurality of metal oxide film pieces 70 is formed of TiO 2 , and each of the plurality of metal oxide film pieces 72 is formed of MgO. That is, the metal oxide film 62 is formed of two different metal oxides. Each of the plurality of metal oxide film pieces 70 and 72 is formed in a circular shape in plan view. The number of each of the plurality of metal oxide film pieces 70 is greater than the number of each of the plurality of metal oxide film pieces 72. In addition, the diameter of each of the plurality of metal oxide film pieces 70 is larger than the diameter of each of the plurality of metal oxide film pieces 72. The diameter of each of the plurality of metal oxide film pieces 70 is the same as the diameter of each of the plurality of metal oxide film pieces 54. Region 66 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 70, and a plurality of metal oxide film pieces 72. The metal oxide film 62 affects the pH gradation in the region 66. Therefore, the pH gradation of the region 66 is the pH gradation (for example, pH 4) formed by the plurality of metal oxide film pieces 70, the pH gradation (for example, pH 7) formed by the plurality of metal oxide film pieces 72, and pure water Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thereby, the pH gradation of the liquid 18 (see FIG. 1) in the vicinity of the region 66 corresponds to the pH 5.5 (the example of the fifth isoelectric point) of the pH 5.5 of the sample 4 Of the pH of
　なお、ＴｉＯ２及びＭｇＯで形成された金属酸化膜６２では、ＴｉＯ２のみで形成された金属酸化膜２８と比べて、金属酸化膜６２の表面に存在するＯＨ基が液体１８中の水素イオン（Ｈ＋）を受け取る量が多くなる。そのため、領域６６により形成されるｐＨ階調は、領域４２により形成されるｐＨ階調よりもアルカリ性寄り（例えばｐＨ５．５）になる。 In the metal oxide film 62 formed of TiO 2 and MgO, the OH group present on the surface of the metal oxide film 62 is a hydrogen ion in the liquid 18 (compared to the metal oxide film 28 formed only of TiO 2 ). The amount of receiving H + ) increases. Therefore, the pH gradation formed by the area 66 is more alkaline (for example, pH 5.5) than the pH gradation formed by the area 42.
　金属酸化膜６４は、担体２０の領域６８において格子状に点在された複数の金属酸化膜片７４（第３の金属酸化膜片の一例）及び複数の金属酸化膜片７６（第４の金属酸化膜片の一例）を有している。担体２０を平面視した際に、複数の金属酸化膜片７４の各々は、複数の金属酸化膜片７６の各々と互いに重ならないように配置されている。 The metal oxide film 64 includes a plurality of metal oxide film pieces 74 (an example of a third metal oxide film piece) and a plurality of metal oxide film pieces 76 (a fourth metal) which are scattered in a lattice shape in the region 68 of the carrier 20. (An example of an oxide film piece). When the carrier 20 is viewed in plan, each of the plurality of metal oxide film pieces 74 is arranged so as not to overlap with each of the plurality of metal oxide film pieces 76.
　複数の金属酸化膜片７４の各々はＴｉＯ２で形成され、複数の金属酸化膜片７６の各々はＭｇＯで形成されている。すなわち、金属酸化膜６４は、異なる２種類の金属酸化物で形成されている。複数の金属酸化膜片７４及び７６の各々は、平面視で円形状に形成されている。複数の金属酸化膜片７４の各々の数は、複数の金属酸化膜片７６の各々の数よりも少ない。また、複数の金属酸化膜片７４の各々の直径は、複数の金属酸化膜片７６の各々の直径よりも小さい。なお、複数の金属酸化膜片７６の各々の直径は、複数の金属酸化膜片６０の各々の直径と同一である。領域６８は、担体２０が露出している部分と、複数の金属酸化膜片７４と、複数の金属酸化膜片７６とにより形成される。金属酸化膜６４は、領域６８におけるｐＨ階調に影響を及ぼす。そのため、領域６８のｐＨ階調は、複数の金属酸化膜片７４が形成するｐＨ階調（例えばｐＨ４）と、複数の金属酸化膜片７６が形成するｐＨ階調（例えば１０ｐＨ）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域６８の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ８．５の等電点（第６の等電点の一例）に対応するｐＨ８．５（第６のｐＨの一例）となる。 Each of the plurality of metal oxide film pieces 74 is formed of TiO 2 , and each of the plurality of metal oxide film pieces 76 is formed of MgO. That is, the metal oxide film 64 is formed of two different metal oxides. Each of the plurality of metal oxide film pieces 74 and 76 is formed in a circular shape in plan view. The number of each of the plurality of metal oxide film pieces 74 is smaller than the number of each of the plurality of metal oxide film pieces 76. In addition, the diameter of each of the plurality of metal oxide film pieces 74 is smaller than the diameter of each of the plurality of metal oxide film pieces 76. The diameter of each of the plurality of metal oxide film pieces 76 is the same as the diameter of each of the plurality of metal oxide film pieces 60. Region 68 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 74, and a plurality of metal oxide film pieces 76. The metal oxide film 64 affects the pH gradation in the region 68. Therefore, the pH gradation of the region 68 includes pH gradation (for example, pH 4) formed by the plurality of metal oxide film pieces 74, pH gradation (for example, 10 pH) formed by the plurality of metal oxide film pieces 76, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thereby, the pH gradation of the liquid 18 in the vicinity of the region 68 is, for example, one example of the pH 8.5 (corresponding to one example of the sixth isoelectric point) of pH 8.5 of the sample 4 ).
　なお、ＴｉＯ２及びＭｇＯで形成された金属酸化膜６４では、ＭｇＯのみで形成された金属酸化膜３６と比べて、金属酸化膜６４の表面に存在するＯＨ基が液体１８中に水素イオン（Ｈ＋）を放出する量が多くなる。そのため、領域６８により形成されるｐＨ階調は、領域５０により形成されるｐＨ階調よりも酸性寄り（例えばｐＨ８．５）になる。 In the metal oxide film 64 formed of TiO 2 and MgO, the OH group present on the surface of the metal oxide film 64 is a hydrogen ion (H (H) as compared to the metal oxide film 36 formed only of MgO. The amount of releasing + ) increases. Therefore, the pH gradation formed by the region 68 is more acidic (for example, pH 8.5) than the pH gradation formed by the region 50.
　図５に示すように、複数の金属酸化膜片７０の各々の数は、複数の金属酸化膜片７４の各々の数よりも多く、複数の金属酸化膜片７０の各々の面積は、複数の金属酸化膜片７４の各々の面積よりも大きい。また、複数の金属酸化膜片７２の各々の数は、複数の金属酸化膜片７６の各々の数よりも少なく、複数の金属酸化膜片７２の各々の面積は、複数の金属酸化膜片７６の各々の面積よりも小さい。なお、複数の金属酸化膜片７０の各々と複数の金属酸化膜片７４の各々とで、面積及び数の少なくとも一方が異なっていてもよい。同様に、複数の金属酸化膜片７２の各々と複数の金属酸化膜片７６の各々とで、面積及び数の少なくとも一方が異なっていてもよい。 As shown in FIG. 5, the number of each of the plurality of metal oxide film pieces 70 is larger than the number of each of the plurality of metal oxide film pieces 74, and the area of each of the plurality of metal oxide film pieces 70 is a plurality of It is larger than the area of each of the metal oxide film pieces 74. Further, the number of each of the plurality of metal oxide film pieces 72 is smaller than the number of each of the plurality of metal oxide film pieces 76, and the area of each of the plurality of metal oxide film pieces 72 is a plurality of metal oxide film pieces 76. Less than the area of each of Note that at least one of the area and the number may be different between each of the plurality of metal oxide film pieces 70 and each of the plurality of metal oxide film pieces 74. Similarly, at least one of the area and the number of each of the plurality of metal oxide film pieces 72 and each of the plurality of metal oxide film pieces 76 may be different.
　［３－２．効果］
　上述したように、金属酸化膜６２及び６４の各々を異なる２種類の金属酸化物で形成することにより、ｐＨ勾配のｐＨ階調をより細分化することができる。 [3-2. effect]
As described above, by forming each of the metal oxide films 62 and 64 with two different metal oxides, it is possible to further subdivide the pH gradation of the pH gradient.
　また、担体２０を平面視した際に、金属酸化膜６２の複数の金属酸化膜片７０の各々は、複数の金属酸化膜片７２の各々と互いに重ならないように配置されているので、複数の金属酸化膜片７０及び複数の金属酸化膜片７２の各々と、液体１８との接触面積を十分に確保することができる。その結果、複数の金属酸化膜片７０及び複数の金属酸化膜片７２の各々の、液体１８に対する酸化還元反応を効率良く生じさせることができる。 Further, when the carrier 20 is viewed in plan, each of the plurality of metal oxide film pieces 70 of the metal oxide film 62 is arranged so as not to overlap with each of the plurality of metal oxide film pieces 72. A contact area between each of the metal oxide film pieces 70 and the plurality of metal oxide film pieces 72 and the liquid 18 can be sufficiently secured. As a result, the redox reaction of the plurality of metal oxide film pieces 70 and the plurality of metal oxide film pieces 72 with respect to the liquid 18 can be efficiently generated.
　（実施の形態４）
　［４－１．金属酸化膜体の構成］
　次に、図６～図８を参照しながら、実施の形態４に係る電気泳動支持体８Ｃの金属酸化膜体２２Ｃの構成について説明する。図６は、実施の形態４に係る電気泳動支持体８Ｃの金属酸化膜体２２Ｃの構成を示す上面図である。図７は、図６のＶＩＩ－ＶＩＩ線による、実施の形態４に係る電気泳動支持体８Ｃの要部断面図である。図８は、図６のＶＩＩＩ－ＶＩＩＩ線による、実施の形態４に係る電気泳動支持体８Ｃの要部断面図である。 Embodiment 4
[4-1. Configuration of metal oxide film]
Next, the configuration of the metal oxide film body 22C of the electrophoresis support 8C according to the fourth embodiment will be described with reference to FIG. 6 to FIG. FIG. 6 is a top view showing the configuration of the metal oxide film 22C of the electrophoresis support 8C according to the fourth embodiment. FIG. 7 is a cross-sectional view of an essential part of the electrophoresis support 8C according to the fourth embodiment, taken along the line VII-VII in FIG. FIG. 8 is a cross-sectional view of an essential portion of the electrophoresis support 8C according to the fourth embodiment, taken along line VIII-VIII in FIG.
　図６に示すように、実施の形態４に係る電気泳動支持体８Ｃでは、金属酸化膜体２２Ｃの構成が上記実施の形態１と異なっている。具体的には、金属酸化膜体２２Ｃは、複数の金属酸化膜２６～３８に加えて、金属酸化膜７８及び８０（第５の金属酸化膜の一例）を有している。また、担体２０の領域４２と領域４４との間には領域８２（第５の領域の一例）が配置され、担体２０の領域４８と領域５０との間には領域８４（第５の領域の一例）が配置されている。 As shown in FIG. 6, in the electrophoresis support 8C according to the fourth embodiment, the configuration of the metal oxide film 22C is different from that of the first embodiment. Specifically, the metal oxide film body 22 C has metal oxide films 78 and 80 (an example of a fifth metal oxide film) in addition to the plurality of metal oxide films 26 to 38. In addition, a region 82 (an example of a fifth region) is disposed between the region 42 of the carrier 20 and the region 44, and a region 84 (of the fifth region) is disposed between the region 48 and the region 50 of the carrier 20. An example is arranged.
　金属酸化膜７８は、担体２０の領域８２において格子状に点在された複数の金属酸化膜片８６（第１の金属酸化膜片の一例）及び複数の金属酸化膜片８８（第２の金属酸化膜片の一例）を有している。図６及び図７に示すように、担体２０を平面視した際に、複数の金属酸化膜片８６の各々は、複数の金属酸化膜片８８の各々と互いに重なるように配置されている。 The metal oxide film 78 includes a plurality of metal oxide film pieces 86 (an example of a first metal oxide film piece) and a plurality of metal oxide film pieces 88 (a second metal) scattered in a lattice shape in the region 82 of the carrier 20. (An example of an oxide film piece). As shown in FIGS. 6 and 7, each of the plurality of metal oxide film pieces 86 is arranged to overlap with each of the plurality of metal oxide film pieces 88 when the carrier 20 is viewed in plan.
　複数の金属酸化膜片８６の各々はＴｉＯ２で形成され、複数の金属酸化膜片８８の各々はＭｇＯで形成されている。すなわち、金属酸化膜７８は、異なる２種類の金属酸化物で形成されている。複数の金属酸化膜片８６及び８８の各々は、平面視で円形状に形成されている。複数の金属酸化膜片８６の各々の数及び直径はそれぞれ、複数の金属酸化膜片８８の各々の数及び直径と同一である。図７に示すように、複数の金属酸化膜片８６の各々は、複数の金属酸化膜片８８の各々の上側に積層されている。領域８２は、担体２０が露出している部分と、複数の金属酸化膜片８６と、複数の金属酸化膜片８８とにより形成される。金属酸化膜７８は、領域８２におけるｐＨ階調に影響を及ぼす。そのため、領域８２のｐＨ階調は、複数の金属酸化膜片８６が形成するｐＨ階調（例えばｐＨ４）と、複数の金属酸化膜片８８が形成するｐＨ階調（例えば７ｐＨ）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域８２の近傍における液体１８（図１参照）のｐＨ階調は、例えば試料４のｐＨ５．５の等電点（第５の等電点の一例）に対応するｐＨ５．５（第５のｐＨの一例）となる。 Each of the plurality of metal oxide film pieces 86 is formed of TiO 2 , and each of the plurality of metal oxide film pieces 88 is formed of MgO. That is, the metal oxide film 78 is formed of two different metal oxides. Each of the plurality of metal oxide film pieces 86 and 88 is formed in a circular shape in plan view. The number and diameter of each of the plurality of metal oxide film pieces 86 are the same as the number and diameter of each of the plurality of metal oxide film pieces 88, respectively. As shown in FIG. 7, each of the plurality of metal oxide film pieces 86 is stacked on the upper side of each of the plurality of metal oxide film pieces 88. Region 82 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 86, and a plurality of metal oxide film pieces 88. The metal oxide film 78 affects the pH gradation in the region 82. Therefore, the pH gradation of the region 82 includes pH gradation (for example, pH 4) formed by the plurality of metal oxide film pieces 86, pH gradation (for example, 7 pH) formed by the plurality of metal oxide film pieces 88, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thus, the pH gradation of the liquid 18 (see FIG. 1) in the vicinity of the region 82 is, for example, pH 5.5 (the fifth isoelectric point) corresponding to the pH 5.5 of the sample 4 An example of the pH of 5).
　金属酸化膜８０は、担体２０の領域８４において格子状に点在された複数の金属酸化膜片９０（第１の金属酸化膜片の一例）及び複数の金属酸化膜片９２（第２の金属酸化膜片の一例）を有している。図６及び図８に示すように、担体２０を平面視した際に、複数の金属酸化膜片９０の各々は、複数の金属酸化膜片９２の各々と互いに重なるように配置されている。 The metal oxide film 80 includes a plurality of metal oxide film pieces 90 (an example of a first metal oxide film piece) and a plurality of metal oxide film pieces 92 (a second metal) which are scattered in a lattice shape in the region 84 of the carrier 20. (An example of an oxide film piece). As shown in FIGS. 6 and 8, each of the plurality of metal oxide film pieces 90 is arranged to overlap with each of the plurality of metal oxide film pieces 92 when the carrier 20 is viewed in plan.
　複数の金属酸化膜片９０の各々はＴｉＯ２で形成され、複数の金属酸化膜片９２の各々はＭｇＯで形成されている。すなわち、金属酸化膜８０は、異なる２種類の金属酸化物で形成されている。複数の金属酸化膜片９０及び９２の各々は、平面視で円形状に形成されている。複数の金属酸化膜片９０の各々の数及び直径はそれぞれ、複数の金属酸化膜片９２の各々の数及び直径と同一である。図８に示すように、複数の金属酸化膜片９２の各々は、複数の金属酸化膜片９０の各々の上側に積層されている。領域８４は、担体２０が露出している部分と、複数の金属酸化膜片９０と、複数の金属酸化膜片９２とにより形成される。金属酸化膜８０は、領域８４におけるｐＨ階調に影響を及ぼす。そのため、領域８４のｐＨ階調は、複数の金属酸化膜片９０が形成するｐＨ階調（例えばｐＨ４）と、複数の金属酸化膜片９２が形成するｐＨ階調（例えばｐＨ１０）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域８４の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ８．５の等電点（第５の等電点の一例）に対応するｐＨ８．５（第５のｐＨの一例）となる。 Each of the plurality of metal oxide film pieces 90 is formed of TiO 2 , and each of the plurality of metal oxide film pieces 92 is formed of MgO. That is, the metal oxide film 80 is formed of two different metal oxides. Each of the plurality of metal oxide film pieces 90 and 92 is formed in a circular shape in plan view. The number and diameter of each of the plurality of metal oxide film pieces 90 is the same as the number and diameter of each of the plurality of metal oxide film pieces 92. As shown in FIG. 8, each of the plurality of metal oxide film pieces 92 is stacked on the upper side of each of the plurality of metal oxide film pieces 90. Region 84 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 90, and a plurality of metal oxide film pieces 92. The metal oxide film 80 affects the pH gradation in the region 84. Therefore, the pH gradation of the region 84 includes pH gradation (for example, pH 4) formed by the plurality of metal oxide film pieces 90, pH gradation (for example, pH 10) formed by the plurality of metal oxide film pieces 92, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thereby, the pH gradation of the liquid 18 in the vicinity of the region 84 is, for example, one example of the pH 8.5 (corresponding to one example of the fifth isoelectric point) of pH 8.5 of the sample 4 ).
　［４－２．効果］
　本実施の形態では、金属酸化膜７８及び８０の各々を異なる２種類の金属酸化物で形成することにより、ｐＨ勾配のｐＨ階調をより細分化することができる。 [4-2. effect]
In this embodiment, the pH gradation of the pH gradient can be further subdivided by forming each of the metal oxide films 78 and 80 with two different metal oxides.
　また、担体２０を平面視した際に、金属酸化膜７８の複数の金属酸化膜片８６の各々は、複数の金属酸化膜片８８の各々と互いに重なるように配置されているので、担体２０の領域８２の面積を十分に確保できない場合であっても、金属酸化膜７８を領域８２にコンパクトに配置することができる。 Further, each of the plurality of metal oxide film pieces 86 of the metal oxide film 78 is disposed so as to overlap with each of the plurality of metal oxide film pieces 88 when the carrier 20 is viewed in a plan view. Even if the area of the region 82 can not be sufficiently secured, the metal oxide film 78 can be compactly disposed in the region 82.
　（実施の形態５）
　［５－１．金属酸化膜体の構成］
　次に、図９～図１１を参照しながら、実施の形態５に係る電気泳動支持体８Ｄの金属酸化膜体２２Ｄの構成について説明する。図９は、実施の形態５に係る電気泳動支持体８Ｄの金属酸化膜体２２Ｄの構成を示す上面図である。図１０は、実施の形態５に係る電気泳動支持体８Ｄの金属酸化膜体２２Ｄの成膜工程の一例を説明するための図である。図１１は、実施の形態５に係る電気泳動支持体８Ｄの金属酸化膜体２２Ｄにより形成されるｐＨ勾配を説明するためのグラフである。 Fifth Embodiment
[5-1. Configuration of metal oxide film]
Next, the configuration of the metal oxide film body 22D of the electrophoresis support 8D according to the fifth embodiment will be described with reference to FIGS. 9 to 11. FIG. FIG. 9 is a top view showing the structure of a metal oxide film body 22D of an electrophoresis support 8D according to the fifth embodiment. FIG. 10 is a view for explaining an example of the film forming step of the metal oxide film 22D of the electrophoresis support 8D according to the fifth embodiment. FIG. 11 is a graph for explaining the pH gradient formed by the metal oxide film body 22D of the electrophoresis support 8D according to the fifth embodiment.
　図９に示すように、実施の形態５に係る電気泳動支持体８Ｄでは、金属酸化膜体２２Ｄの構成が上記実施の形態１と異なっている。具体的には、金属酸化膜体２２Ｄは、複数の金属酸化膜９４～１０２を有している。複数の金属酸化膜９４～１０２はそれぞれ、担体２０の上面２４における複数の領域１０４～１１２に配置されている。複数の領域１０４～１１２は、担体２０の長手方向（Ｘ軸方向）に沿って、この順に一列に並んで配置されている。 As shown in FIG. 9, in the electrophoresis support 8D according to the fifth embodiment, the configuration of the metal oxide film 22D is different from that of the first embodiment. Specifically, the metal oxide film body 22D has a plurality of metal oxide films 94 to 102. The plurality of metal oxide films 94 to 102 are respectively disposed in the plurality of regions 104 to 112 on the upper surface 24 of the carrier 20. The plurality of regions 104 to 112 are arranged in line in this order along the longitudinal direction (X-axis direction) of the carrier 20.
　金属酸化膜９４は、ＴｉＯ２で形成されており、担体２０の領域１０４の全域に亘って均一に配置されている。金属酸化膜９４は、例えばｐＨ４のｐＨ階調を形成する。このような金属酸化膜９４が領域１０４の全域に亘って配置されることにより、領域１０４の近傍における液体１８（図１参照）のｐＨ階調は、例えば試料４のｐＨ４の等電点に対応するｐＨ４となる。 The metal oxide film 94 is formed of TiO 2 , and is uniformly disposed over the entire region 104 of the carrier 20. The metal oxide film 94 forms, for example, a pH gradation of pH4. By arranging such a metal oxide film 94 over the entire region 104, the pH gradation of the liquid 18 (see FIG. 1) in the vicinity of the region 104 corresponds to, for example, the isoelectric point of pH 4 of the sample 4. PH 4 to
　金属酸化膜９６は、担体２０の領域１０６において格子状に点在された複数の金属酸化膜片１１４及び複数の金属酸化膜片１１６を有している。担体２０を平面視した際に、複数の金属酸化膜片１１４の各々は、複数の金属酸化膜片１１６の各々と互いに重なるように配置されている。 The metal oxide film 96 has a plurality of metal oxide film pieces 114 and a plurality of metal oxide film pieces 116 interspersed in a lattice shape in the region 106 of the carrier 20. When the carrier 20 is viewed in plan, each of the plurality of metal oxide film pieces 114 is arranged to overlap with each of the plurality of metal oxide film pieces 116.
　複数の金属酸化膜片１１４の各々はＴｉＯ２で形成され、複数の金属酸化膜片１１６の各々はＭｇＯで形成されている。すなわち、金属酸化膜９６は、異なる２種類の金属酸化物で形成されている。複数の金属酸化膜片１１４及び１１６の各々は、平面視で円形状に形成されている。複数の金属酸化膜片１１４の各々の数は、複数の金属酸化膜片１１６の各々の数と同一である。また、複数の金属酸化膜片１１４の各々の直径は、複数の金属酸化膜片１１６の各々の直径よりも大きい。複数の金属酸化膜片１１４の各々は、複数の金属酸化膜片１１６の各々の下側に積層されている。領域１０６は、担体２０が露出している部分と、複数の金属酸化膜片１１４と、複数の金属酸化膜片１１６とにより形成される。金属酸化膜９６は、領域１０６におけるｐＨ階調に影響を及ぼす。そのため、領域１０６のｐＨ階調は、複数の金属酸化膜片１１４が形成するｐＨ階調（例えばｐＨ４）と、複数の金属酸化膜片１１６が形成するｐＨ階調（例えばｐＨ７）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域１０６の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ５．５の等電点に対応するｐＨ５．５となる。 Each of the plurality of metal oxide film pieces 114 is formed of TiO 2 , and each of the plurality of metal oxide film pieces 116 is formed of MgO. That is, the metal oxide film 96 is formed of two different metal oxides. Each of the plurality of metal oxide film pieces 114 and 116 is formed in a circular shape in plan view. The number of each of the plurality of metal oxide film pieces 114 is the same as the number of each of the plurality of metal oxide film pieces 116. In addition, the diameter of each of the plurality of metal oxide film pieces 114 is larger than the diameter of each of the plurality of metal oxide film pieces 116. Each of the plurality of metal oxide film pieces 114 is stacked under each of the plurality of metal oxide film pieces 116. Region 106 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 114 and a plurality of metal oxide film pieces 116. The metal oxide film 96 affects the pH gradation in the region 106. Therefore, the pH gradation of the region 106 includes pH gradation (for example, pH 4) formed by a plurality of metal oxide film pieces 114, pH gradation (for example, pH 7) formed by a plurality of metal oxide film pieces 116, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thereby, the pH gradation of the liquid 18 in the vicinity of the region 106 becomes, for example, pH 5.5 corresponding to the isoelectric point of pH 5.5 of the sample 4.
　金属酸化膜９８は、担体２０の領域１０８において格子状に点在された複数の金属酸化膜片１１８及び複数の金属酸化膜片１２０を有している。担体２０を平面視した際に、複数の金属酸化膜片１１８の各々は、複数の金属酸化膜片１２０の各々と互いに重なるように配置されている。 The metal oxide film 98 has a plurality of metal oxide film pieces 118 and a plurality of metal oxide film pieces 120 interspersed in a lattice shape in the region 108 of the carrier 20. When the carrier 20 is viewed in plan, each of the plurality of metal oxide film pieces 118 is arranged to overlap with each of the plurality of metal oxide film pieces 120.
　複数の金属酸化膜片１１８の各々はＴｉＯ２で形成され、複数の金属酸化膜片１２０の各々はＭｇＯで形成されている。すなわち、金属酸化膜９８は、異なる２種類の金属酸化物で形成されている。複数の金属酸化膜片１１８及び１２０の各々は、平面視で円形状に形成されている。複数の金属酸化膜片１１８の各々の数及び直径はそれぞれ、複数の金属酸化膜片１２０の各々の数及び直径と同一である。複数の金属酸化膜片１１８の各々は、複数の金属酸化膜片１２０の各々の下側に積層されている。領域１０８は、担体２０が露出している部分と、複数の金属酸化膜片１１８と、複数の金属酸化膜片１２０とにより形成される。金属酸化膜９８は、領域１０８におけるｐＨ階調に影響を及ぼす。そのため、領域１０８のｐＨ階調は、複数の金属酸化膜片１１８が形成するｐＨ階調（例えばｐＨ４）と、複数の金属酸化膜片１２０が形成するｐＨ階調（例えばｐＨ７）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域１０８の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ７の等電点に対応するｐＨ７となる。 Each of the plurality of metal oxide film pieces 118 is formed of TiO 2 , and each of the plurality of metal oxide film pieces 120 is formed of MgO. That is, the metal oxide film 98 is formed of two different metal oxides. Each of the plurality of metal oxide film pieces 118 and 120 is formed in a circular shape in plan view. The number and diameter of each of the plurality of metal oxide film pieces 118 are the same as the number and diameter of each of the plurality of metal oxide film pieces 120. Each of the plurality of metal oxide film pieces 118 is stacked under each of the plurality of metal oxide film pieces 120. Region 108 is formed of a portion where carrier 20 is exposed, a plurality of metal oxide film pieces 118, and a plurality of metal oxide film pieces 120. The metal oxide film 98 affects the pH gradation in the region 108. Therefore, the pH gradation of the region 108 includes pH gradation (for example, pH 4) formed by a plurality of metal oxide film pieces 118, pH gradation (for example, pH 7) formed by a plurality of metal oxide film pieces 120, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thereby, the pH gradation of the liquid 18 in the vicinity of the region 108 becomes, for example, pH 7 corresponding to the isoelectric point of pH 7 of the sample 4.
　金属酸化膜１００は、担体２０の領域１１０において格子状に点在された複数の金属酸化膜片１２２及び複数の金属酸化膜片１２４を有している。担体２０を平面視した際に、複数の金属酸化膜片１２２の各々は、複数の金属酸化膜片１２４の各々と互いに重なるように配置されている。 The metal oxide film 100 has a plurality of metal oxide film pieces 122 and a plurality of metal oxide film pieces 124 scattered in a lattice shape in the region 110 of the carrier 20. When the carrier 20 is viewed in plan, each of the plurality of metal oxide film pieces 122 is arranged to overlap with each of the plurality of metal oxide film pieces 124.
　複数の金属酸化膜片１２２の各々はＴｉＯ２で形成され、複数の金属酸化膜片１２４の各々はＭｇＯで形成されている。すなわち、金属酸化膜１００は、異なる２種類の金属酸化物で形成されている。複数の金属酸化膜片１２２及び１２４の各々は、平面視で円形状に形成されている。複数の金属酸化膜片１２２の各々の数は、複数の金属酸化膜片１２４の各々の数と同一である。また、複数の金属酸化膜片１２２の各々の直径は、複数の金属酸化膜片１２４の各々の直径よりも小さい。複数の金属酸化膜片１２２の各々は、複数の金属酸化膜片１２４の各々の下側に積層されている。領域１１０は、担体２０が露出している部分と、複数の金属酸化膜片１２２と、複数の金属酸化膜片１２４とにより形成される。金属酸化膜１００は、領域１１０におけるｐＨ階調に影響を及ぼす。そのため、領域１１０のｐＨ階調は、複数の金属酸化膜片１２２が形成するｐＨ階調（例えばｐＨ４）と、複数の金属酸化膜片１２４が形成するｐＨ階調（例えばｐＨ１０）と、純水が形成するｐＨ階調（例えばｐＨ７）との組み合わせにより形成される。これにより、領域１１０の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ８．５の等電点に対応するｐＨ８．５となる。 Each of the plurality of metal oxide film pieces 122 is formed of TiO 2 , and each of the plurality of metal oxide film pieces 124 is formed of MgO. That is, the metal oxide film 100 is formed of two different metal oxides. Each of the plurality of metal oxide film pieces 122 and 124 is formed in a circular shape in plan view. The number of each of the plurality of metal oxide film pieces 122 is the same as the number of each of the plurality of metal oxide film pieces 124. In addition, the diameter of each of the plurality of metal oxide film pieces 122 is smaller than the diameter of each of the plurality of metal oxide film pieces 124. Each of the plurality of metal oxide film pieces 122 is stacked under each of the plurality of metal oxide film pieces 124. The region 110 is formed by a portion where the carrier 20 is exposed, a plurality of metal oxide film pieces 122, and a plurality of metal oxide film pieces 124. The metal oxide film 100 affects the pH gradation in the region 110. Therefore, the pH gradation of the region 110 includes pH gradation (for example, pH 4) formed by a plurality of metal oxide film pieces 122, pH gradation (for example, pH 10) formed by a plurality of metal oxide film pieces 124, and pure water. Is formed in combination with the pH gradation (for example, pH 7) which is formed. Thereby, the pH gradation of the liquid 18 in the vicinity of the region 110 becomes, for example, pH 8.5 corresponding to the isoelectric point of pH 8.5 of the sample 4.
　金属酸化膜１０２は、ＭｇＯで形成されており、担体２０の領域１１２の全域に亘って均一に配置されている。金属酸化膜１０２は、例えばｐＨ１０のｐＨ階調を形成する。このような金属酸化膜１０２が領域１１２の全域に亘って配置されることにより、領域１１２の近傍における液体１８のｐＨ階調は、例えば試料４のｐＨ１０の等電点に対応するｐＨ１０となる。 The metal oxide film 102 is formed of MgO and is uniformly disposed over the entire region 112 of the carrier 20. The metal oxide film 102 forms, for example, a pH gradation of pH 10. By arranging such a metal oxide film 102 over the entire region 112, the pH gradation of the liquid 18 in the vicinity of the region 112 becomes, for example, pH 10 corresponding to the isoelectric point of pH 10 of the sample 4.
　次に、図１０を参照しながら、上述した金属酸化膜体２２Ｄの成膜工程の一例について説明する。まず、図１０の（ａ）に示すように、第１の成膜工程が行われる。第１の成膜工程では、担体２０の上面２４をメタルマスク１２６で覆った状態で、ＡＬＤ法によりＴｉの有機金属化合物ガスを投入する。メタルマスク１２６は、矩形状の孔１２８ａが形成された領域１３０ａと、円形状の複数の孔１２８ｂが形成された領域１３０ｂと、円形状の複数の孔１２８ｃが形成された領域１３０ｃと、円形状の複数の孔１２８ｄが形成された領域１３０ｄと、閉塞された領域１３０ｅとを有している。 Next, with reference to FIG. 10, an example of the film forming process of the metal oxide film 22D described above will be described. First, as shown to (a) of FIG. 10, a 1st film-forming process is performed. In the first film forming step, in a state where the upper surface 24 of the carrier 20 is covered with the metal mask 126, an organometallic compound gas of Ti is introduced by the ALD method. The metal mask 126 includes a region 130a in which the rectangular holes 128a are formed, a region 130b in which the plurality of circular holes 128b are formed, a region 130c in which the plurality of circular holes 128c is formed, and a circular shape A plurality of holes 128d are formed in a region 130d and a closed region 130e.
　複数の孔１２８ｂ、複数の孔１２８ｃ及び複数の孔１２８ｄの各々は、格子状に配置されている。複数の孔１２８ｂ、複数の孔１２８ｃ及び複数の孔１２８ｄの各々の数は同一である。複数の孔１２８ｂの各々の直径は、複数の孔１２８ｃの各々の直径よりも大きい。また、複数の孔１２８ｃの各々の直径は、複数の孔１２８ｄの各々の直径よりも大きい。 Each of the plurality of holes 128b, the plurality of holes 128c, and the plurality of holes 128d is arranged in a lattice. The numbers of the plurality of holes 128b, the plurality of holes 128c, and the plurality of holes 128d are the same. The diameter of each of the plurality of holes 128b is larger than the diameter of each of the plurality of holes 128c. Also, the diameter of each of the plurality of holes 128c is larger than the diameter of each of the plurality of holes 128d.
　これにより、図１０の（ａ）に示すように、担体２０の領域１０４には、メタルマスク１２６の孔１２８ａを通して投入されたＴｉの有機金属化合物ガスにより、金属酸化膜９４が形成される。また、担体２０の領域１０６には、メタルマスク１２６の複数の孔１２８ｂを通して投入されたＴｉの有機金属化合物ガスにより、複数の金属酸化膜片１１４が形成される。また、担体２０の領域１０８には、メタルマスク１２６の複数の孔１２８ｃを通して投入されたＴｉの有機金属化合物ガスにより、複数の金属酸化膜片１１８が形成される。また、担体２０の領域１１０には、メタルマスク１２６の複数の孔１２８ｄを通して投入されたＴｉの有機金属化合物ガスにより、複数の金属酸化膜片１２２が形成される。なお、担体２０の領域１１２は、メタルマスク１２６の領域１３０ｅで完全に覆われているため、ＴｉＯ２の金属酸化膜は形成されない。 As a result, as shown in FIG. 10A, the metal oxide film 94 is formed in the region 104 of the carrier 20 by the organometallic compound gas of Ti introduced through the holes 128a of the metal mask 126. Further, a plurality of metal oxide film pieces 114 are formed in the region 106 of the carrier 20 by the metal organic metal compound gas of Ti introduced through the plurality of holes 128 b of the metal mask 126. Further, a plurality of metal oxide film pieces 118 are formed in the region 108 of the carrier 20 by the metal organic metal compound gas of Ti introduced through the plurality of holes 128 c of the metal mask 126. Further, a plurality of metal oxide film pieces 122 are formed in the region 110 of the carrier 20 by the organometallic compound gas of Ti introduced through the plurality of holes 128 d of the metal mask 126. Since the region 112 of the carrier 20 is completely covered by the region 130e of the metal mask 126, the metal oxide film of TiO 2 is not formed.
　その後、図１０の（ｂ）に示すように、第２の成膜工程が行われる。第２の成膜工程では、担体２０の上面２４をメタルマスク１２６で覆った状態で、ＡＬＤ法によりＭｇの有機金属化合物ガスを投入する。この時、メタルマスク１２６の向きは、第１の成膜工程から左右反転されている。 Thereafter, as shown in (b) of FIG. 10, a second film forming process is performed. In the second film forming step, in a state in which the upper surface 24 of the carrier 20 is covered with the metal mask 126, an organic metal compound gas of Mg is introduced by the ALD method. At this time, the direction of the metal mask 126 is reversed from the first film forming process.
　これにより、図１０の（ｂ）に示すように、担体２０の領域１１２には、メタルマスク１２６の孔１２８ａを通して投入されたＭｇの有機金属化合物ガスにより、金属酸化膜１０２が形成される。また、担体２０の領域１１０には、メタルマスク１２６の複数の孔１２８ｂを通して投入されたＭｇの有機金属化合物ガスにより、複数の金属酸化膜片１２４が複数の金属酸化膜片１２２の上側に積層されるように形成される。また、担体２０の領域１０８には、メタルマスク１２６の複数の孔１２８ｃを通して投入されたＭｇの有機金属化合物ガスにより、複数の金属酸化膜片１２０が複数の金属酸化膜片１１８の上側に積層されるように形成される。また、担体２０の領域１０６には、メタルマスク１２６の複数の孔１２８ｄを通して投入されたＭｇの有機金属化合物ガスにより、複数の金属酸化膜片１１６が複数の金属酸化膜片１１４の上側に積層されるように形成される。なお、担体２０の領域１０４は、メタルマスク１２６の領域１３０ｅで完全に覆われているため、ＭｇＯの金属酸化膜は形成されない。 Thereby, as shown in (b) of FIG. 10, the metal oxide film 102 is formed in the region 112 of the carrier 20 by the Mg organic metal compound gas introduced through the holes 128a of the metal mask 126. Further, in the region 110 of the carrier 20, a plurality of metal oxide film pieces 124 are stacked on the plurality of metal oxide film pieces 122 by metal organic metal compound gas introduced through the plurality of holes 128b of the metal mask 126. Formed to Further, in the region 108 of the carrier 20, the plurality of metal oxide film pieces 120 are stacked on the plurality of metal oxide film pieces 118 by the metal organic metal compound gas of Mg introduced through the plurality of holes 128c of the metal mask 126. Formed to Further, a plurality of metal oxide film pieces 116 are stacked on the plurality of metal oxide film pieces 114 in the region 106 of the carrier 20 by the metal organic metal compound gas of Mg introduced through the plurality of holes 128 d of the metal mask 126. Formed to Since the region 104 of the carrier 20 is completely covered with the region 130e of the metal mask 126, a metal oxide film of MgO is not formed.
　以上のようにして、複数の金属酸化膜９４～１０２はそれぞれ、担体２０の複数の領域１０４～１１２に成膜される。 As described above, the plurality of metal oxide films 94 to 102 are formed on the plurality of regions 104 to 112 of the carrier 20, respectively.
　図１１に示すように、以上のようにして形成された金属酸化膜体２２Ｄでは、ＴｉＯ２の金属酸化膜９４、複数の金属酸化膜片１１４，１１８及び１２２により形成されたｐＨ４～ｐＨ７のｐＨ勾配と、ＭｇＯの金属酸化膜１０２、複数の金属酸化膜片１１６，１２０及び１２４により形成されたｐＨ７～ｐＨ１０のｐＨ勾配とが相互に影響し合うことにより、ｐＨ４～ｐＨ１０のｐＨ勾配が形成されるようになる。 As shown in FIG. 11, in the metal oxide film body 22D formed as described above, the pH of pH 4 to pH 7 formed by the metal oxide film 94 of TiO 2 and the plurality of metal oxide film pieces 114, 118 and 122. The gradient and the pH gradient of pH 7 to pH 10 formed by the metal oxide film 102 of MgO and the plurality of metal oxide film fragments 116, 120 and 124 mutually influence to form a pH gradient of pH 4 to pH 10 Become so.
　［５－２．効果］
　上述と同様に、ｐＨ勾配のｐＨ階調の数を増やした場合であっても、電気泳動支持体８Ｄの製造プロセスが複雑になるのを抑制することができる。 5-2. effect]
As described above, even when the number of pH gradations of the pH gradient is increased, the manufacturing process of the electrophoresis support 8D can be prevented from being complicated.
　（変形例）
　以上、一つ又は複数の態様に係る電気泳動支持体及び電気泳動装置について、上記各実施の形態に基づいて説明したが、本発明は、これらの実施の形態に限定されるものではない。本発明の趣旨を逸脱しない限り、当業者が思い付く各種変形を本実施の形態に施したものや、異なる実施の形態又は変形例における構成要素を組み合わせて構築される形態も、一つ又は複数の態様の範囲内に含まれてもよい。 (Modification)
Although the electrophoresis support and the electrophoresis apparatus according to one or more aspects have been described above based on the above embodiments, the present invention is not limited to these embodiments. Unless it deviates from the gist of the present invention, one or more embodiments in which various modifications that a person skilled in the art finds can be made to the present embodiment, and a configuration constructed by combining components in different embodiments or modifications are also one or more. It may be included within the scope of the embodiments.
　上記各実施の形態では、金属酸化膜体２２（２２Ａ，２２Ｂ，２２Ｃ，２２Ｄ）がＴｉＯ２、ＮｉＯ及びＭｇＯのうち少なくとも２つを含むようにしたが、上記以外の金属酸化物を含むようにしてもよい。金属酸化膜体２２（２２Ａ，２２Ｂ，２２Ｃ，２２Ｄ）は、例えばＡｌ２Ｏ３、ＨｆＯ、ＷＯ３、Ｌａ２Ｏ３、Ｂｉ２Ｏ３、ＳｎＯ２、ＺｎＯ、Ｉｎ２Ｏ、Ｆｅ３Ｏ４、ＣｕＯ及びＳｉＯ２等の任意の２種類以上の金属酸化物を含むようにしてもよい。 In each of the above embodiments, the metal oxide film 22 (22A, 22B, 22C, 22D) includes at least two of TiO 2 , NiO and MgO, but it is possible to include metal oxides other than the above. Good. The metal oxide film body 22 (22A, 22B, 22C, 22D) is, for example, Al 2 O 3 , HfO, WO 3 , La 2 O 3 , Bi 2 O 3 , SnO 2 , ZnO, In 2 O, Fe 3 O 4 And any two or more metal oxides such as CuO and SiO 2 may be included.
　上記各実施の形態では、試料４はタンパク質を含むようにしたが、例えばＤＮＡ（デオキシリボ核酸）等を含むようにしてもよい。 In each of the above embodiments, the sample 4 contains a protein, but it may contain, for example, DNA (deoxyribonucleic acid) or the like.
　上記実施の形態１では、ｐＨ４～ｐＨ１０の７つのｐＨ階調を有するｐＨ勾配を形成したが、これに限定されず、例えばｐＨ３～ｐＨ１１の９つのｐＨ階調等、任意の個数のｐＨ階調を有するｐＨ勾配を形成してもよい。 In the first embodiment, although the pH gradient having seven pH gradations of pH 4 to pH 10 is formed, the present invention is not limited to this, for example, any number of pH gradations such as nine pH gradations of pH 3 to pH 11 You may form a pH gradient with
　なお、上記各実施の形態において、各構成要素は、専用のハードウェアで構成されるか、各構成要素に適したソフトウェアプログラムを実行することによって実現されてもよい。各構成要素は、ＣＰＵ又はプロセッサ等のプログラム実行部が、ハードディスク又は半導体メモリ等の記録媒体に記録されたソフトウェアプログラムを読み出して実行することによって実現されてもよい。 In the above embodiments, each component may be configured by dedicated hardware or may be realized by executing a software program suitable for each component. Each component may be realized by a program execution unit such as a CPU or a processor reading out and executing a software program recorded on a recording medium such as a hard disk or a semiconductor memory.
　また、以下のような場合も本発明に含まれる。 Further, the following cases are also included in the present invention.
　（１）上記の各装置は、具体的には、マイクロプロセッサ、ＲＯＭ、ＲＡＭ、ハードディスクユニット、ディスプレイユニット、キーボード、マウスなどから構成されるコンピュータシステムで実現され得る。ＲＡＭ又はハードディスクユニットには、コンピュータプログラムが記憶されている。マイクロプロセッサが、コンピュータプログラムにしたがって動作することにより、各装置は、その機能を達成する。ここでコンピュータプログラムは、所定の機能を達成するために、コンピュータに対する指令を示す命令コードが複数個組み合わされて構成されたものである。 (1) Each of the above-described devices can be specifically realized by a computer system including a microprocessor, a ROM, a RAM, a hard disk unit, a display unit, a keyboard, a mouse and the like. A computer program is stored in the RAM or the hard disk unit. Each device achieves its function by the microprocessor operating according to the computer program. Here, the computer program is configured by combining a plurality of instruction codes indicating instructions to the computer in order to achieve a predetermined function.
　（２）上記の各装置を構成する構成要素の一部又は全部は、１個のシステムＬＳＩ（Ｌａｒｇｅ　Ｓｃａｌｅ　Ｉｎｔｅｇｒａｔｉｏｎ：大規模集積回路）から構成されているとしてもよい。システムＬＳＩは、複数の構成部を１個のチップ上に集積して製造された超多機能ＬＳＩであり、具体的には、マイクロプロセッサ、ＲＯＭ、ＲＡＭなどを含んで構成されるコンピュータシステムである。ＲＯＭには、コンピュータプログラムが記憶されている。マイクロプロセッサが、ＲＯＭからＲＡＭにコンピュータプログラムをロードし、ロードしたコンピュータプログラムにしたがって演算等の動作することにより、システムＬＳＩは、その機能を達成する。 (2) Some or all of the components constituting each of the above-described devices may be configured from one system LSI (Large Scale Integration: large scale integrated circuit). The system LSI is a super-multifunctional LSI manufactured by integrating a plurality of components on one chip, and more specifically, a computer system including a microprocessor, a ROM, a RAM, and the like. . A computer program is stored in the ROM. The system LSI achieves its functions as the microprocessor loads a computer program from the ROM to the RAM and operates according to the loaded computer program.
　（３）上記の各装置を構成する構成要素の一部又は全部は、各装置に脱着可能なＩＣカード又は単体のモジュールから構成されてもよい。ＩＣカード又はモジュールは、マイクロプロセッサ、ＲＯＭ、ＲＡＭなどから構成されるコンピュータシステムである。ＩＣカード又はモジュールには、上記の超多機能ＬＳＩが含まれてもよい。マイクロプロセッサが、コンピュータプログラムにしたがって動作することにより、ＩＣカード又はモジュールは、その機能を達成する。このＩＣカード又はこのモジュールは、耐タンパ性を有してもよい。 (3) A part or all of the components constituting each of the above-described devices may be configured from an IC card or a single module that can be detached from each device. The IC card or module is a computer system including a microprocessor, a ROM, a RAM, and the like. The IC card or module may include the above-described ultra-multifunctional LSI. The IC card or module achieves its function by the microprocessor operating according to the computer program. This IC card or this module may have tamper resistance.
　（４）本発明は、上記に示す方法で実現されてもよい。また、これらの方法をコンピュータにより実現するコンピュータプログラムで実現してもよいし、コンピュータプログラムからなるデジタル信号で実現してもよい。 (4) The present invention may be realized by the method shown above. Also, these methods may be realized by a computer program realized by a computer, or may be realized by a digital signal consisting of a computer program.
　また、本発明は、コンピュータプログラム又はデジタル信号をコンピュータ読み取り可能な記録媒体、例えば、フレキシブルディスク、ハードディスク、ＣＤ－ＲＯＭ、ＭＯ、ＤＶＤ、ＤＶＤ－ＲＯＭ、ＤＶＤ－ＲＡＭ、ＢＤ（Ｂｌｕ－ｒａｙ（登録商標）Ｄｉｓｃ）、半導体メモリなどに記録したもので実現してもよい。また、これらの記録媒体に記録されているデジタル信号で実現してもよい。 Further, the present invention is a computer program or a recording medium which can read digital signals from a computer, such as a flexible disk, a hard disk, a CD-ROM, an MO, a DVD, a DVD-ROM, a DVD-RAM, a BD (Blu-ray (registered trademark) (Disc), a semiconductor memory or the like. Also, it may be realized by digital signals recorded in these recording media.
　また、本発明は、コンピュータプログラム又はデジタル信号を、電気通信回線、無線又は有線通信回線、インターネットを代表とするネットワーク、データ放送等を経由して伝送してもよい。 In addition, the present invention may transmit a computer program or a digital signal via a telecommunication line, a wireless or wired communication line, a network represented by the Internet, data broadcasting, and the like.
　また、本発明は、マイクロプロセッサとメモリを備えたコンピュータシステムであって、メモリは、コンピュータプログラムを記憶しており、マイクロプロセッサは、コンピュータプログラムにしたがって動作してもよい。 Also, the present invention is a computer system comprising a microprocessor and a memory, the memory storing a computer program, and the microprocessor may operate according to the computer program.
　また、プログラム又はデジタル信号を記録媒体に記録して移送することにより、又はプログラム又はデジタル信号をネットワーク等を経由して移送することにより、独立した他のコンピュータシステムにより実施するとしてもよい。 In addition, it may be implemented by another independent computer system by recording and transporting a program or digital signal on a recording medium, or by transporting a program or digital signal via a network or the like.
　（５）上記実施の形態及び上記変形例をそれぞれ組み合わせるとしてもよい。 (5) The above embodiment and the above modification may be combined respectively.
　本発明の電気泳動支持体は、試料を電気泳動するための電気泳動装置等に適用することができる。 The electrophoresis support of the present invention can be applied to an electrophoresis apparatus or the like for electrophoresis of a sample.
２　電気泳動装置
４　試料
４ａ，４ｂ　タンパク質
６　容器
８，８Ａ，８Ｂ，８Ｃ，８Ｄ　電気泳動支持体
１０，１２　電極
１４　電源装置
１６　開口部
１８　液体
２０　担体
２２，２２Ａ，２２Ｂ，２２Ｃ，２２Ｄ　金属酸化膜体
２４　上面
２６，２８，２８Ａ，３０，３０Ａ，３２，３４，３４Ａ，３６，３６Ａ，３８，６２，６４，７８，８０，９４，９６，９８，１００，１０２　金属酸化膜
４０，４２，４２Ａ，４４，４４Ａ，４６，４８，４８Ａ，５０，５０Ａ，５２，６６，６８，８２，８４，１０４，１０６，１０８，１１０，１１２，１３０ａ，１３０ｂ，１３０ｃ，１３０ｄ，１３０ｅ　領域
５４，５６，５８，６０，７０，７２，７４，７６，８６，８８，９０，９２，１１４，１１６，１１８，１２０，１２２，１２４　金属酸化膜片
１２６　メタルマスク
１２８ａ，１２８ｂ，１２８ｃ，１２８ｄ　孔 2 electrophoresis apparatus 4 samples 4a, 4b protein 6 containers 8, 8A, 8B, 8C, 8D electrophoresis support 10, 12 electrode 14 power supply 16 opening 18 liquid 20 carrier 22, 22A, 22B, 22C, 22D metal oxidation Upper surface 26, 28, 28A, 30, 30A, 32, 34, 34A, 36, 36A, 38, 62, 64, 78, 94, 96, 98, 100, 102 Metal oxide films 40, 42, 42A, 44, 44A, 46, 48, 48A, 50, 50A, 52, 66, 68, 82, 84, 106, 108, 110, 112, 130a, 130b, 130c, 130d, 130e regions 54, 56, 58, 60, 70, 72, 74, 76, 68, 88, 90, 92, 114, 116, 118, 120, 122, 124 metal oxide film pieces 1 6 metal mask 128a, 128b, 128c, 128d hole

Claims (11)

  1. 　試料を電気泳動するための電気泳動装置に用いられる電気泳動支持体であって、
    　多孔質状に形成された担体であって、第１の領域と、前記第１の領域に隣接して配置された第２の領域とが表面に形成された担体と、
    　前記試料の第１の等電点に対応する第１のｐＨを形成するための第１の金属酸化膜であって、前記第１の領域に配置され、第１の金属酸化物で形成された第１の金属酸化膜と、
    　前記試料の第２の等電点に対応する第２のｐＨを形成するための第２の金属酸化膜であって、前記第２の領域に配置され、前記第１の金属酸化物で形成された第２の金属酸化膜と、を備え、
    　前記担体を平面視した際に、前記第１の領域において前記第１の金属酸化膜が占める第１の面積割合は、前記第２の領域において前記第２の金属酸化膜が占める第２の面積割合と異なる
    　電気泳動支持体。     An electrophoresis support for use in an electrophoresis apparatus for electrophoresis of a sample, comprising:
    A porous carrier, wherein the first region and the second region disposed adjacent to the first region are formed on the surface;
    A first metal oxide film for forming a first pH corresponding to a first isoelectric point of the sample, wherein the first metal oxide film is disposed in the first region and formed of the first metal oxide A first metal oxide film,
    A second metal oxide film for forming a second pH corresponding to a second isoelectric point of the sample, which is disposed in the second region and formed of the first metal oxide A second metal oxide film,
    When the carrier is viewed in plan, a first area ratio occupied by the first metal oxide film in the first region is a second area occupied by the second metal oxide film in the second region. Electrophoretic support different from proportions.
  2. 　前記担体の前記表面には、さらに、前記第２の領域を挟んで前記第１の領域と反対側に配置された第３の領域が形成され、
    　前記電気泳動支持体は、さらに、前記試料の第３の等電点に対応する第３のｐＨを形成するための第３の金属酸化膜であって、前記第３の領域に配置され、第２の金属酸化物で形成された第３の金属酸化膜を備える
    　請求項１に記載の電気泳動支持体。     Further, a third region is formed on the surface of the carrier opposite to the first region across the second region,
    The electrophoresis support is a third metal oxide film for forming a third pH corresponding to a third isoelectric point of the sample, and is disposed in the third region, The electrophoresis support according to claim 1, comprising a third metal oxide film formed of two metal oxides.
  3. 　前記担体の前記表面には、さらに、前記第３の領域を挟んで前記第２の領域と反対側に配置された第４の領域が形成され、
    　前記電気泳動支持体は、さらに、前記試料の第４の等電点に対応する第４のｐＨを形成するための第４の金属酸化膜であって、前記第４の領域に配置され、前記第２の金属酸化物で形成された第４の金属酸化膜を備え、
    　前記担体を平面視した際に、前記第３の領域において前記第３の金属酸化膜が占める第３の面積割合は、前記第４の領域において前記第４の金属酸化膜が占める第４の面積割合と異なる
    　請求項２に記載の電気泳動支持体。     Further, a fourth region disposed on the opposite side of the second region with respect to the third region is formed on the surface of the carrier.
    The electrophoresis support is further a fourth metal oxide film for forming a fourth pH corresponding to a fourth isoelectric point of the sample, and is disposed in the fourth region, A fourth metal oxide film formed of a second metal oxide,
    When the carrier is viewed in plan, a third area ratio occupied by the third metal oxide film in the third area is a fourth area occupied by the fourth metal oxide film in the fourth area. The electrophoresis support according to claim 2, which is different from the ratio.
  4. 　前記第１の等電点が前記第２の等電点よりも低く、且つ、前記第２の等電点が純水のｐＨに対応する等電点よりも低い場合には、前記第２の面積割合は、前記第１の面積割合よりも小さい
    　請求項１に記載の電気泳動支持体。     If the first isoelectric point is lower than the second isoelectric point and the second isoelectric point is lower than the isoelectric point corresponding to the pH of pure water, then the second The electrophoresis support according to claim 1, wherein the area ratio is smaller than the first area ratio.
  5. 　前記第１の等電点が前記第２の等電点よりも高く、且つ、前記第２の等電点が純水のｐＨに対応する等電点よりも高い場合には、前記第２の面積割合は、前記第１の面積割合よりも小さい
    　請求項１に記載の電気泳動支持体。     If the first isoelectric point is higher than the second isoelectric point and the second isoelectric point is higher than the isoelectric point corresponding to the pH of pure water, then the second The electrophoresis support according to claim 1, wherein the area ratio is smaller than the first area ratio.
  6. 　前記担体の前記表面には、さらに、前記第２の領域と前記第３の領域との間に配置された第５の領域が形成され、
    　前記電気泳動支持体は、さらに、前記試料の第５の等電点に対応する第５のｐＨを形成するための第５の金属酸化膜であって、前記第５の領域に配置され、前記第１の金属酸化物及び前記第２の金属酸化物で形成された第５の金属酸化膜を備える
    　請求項２又は３に記載の電気泳動支持体。     Further, a fifth region disposed between the second region and the third region is formed on the surface of the carrier.
    The electrophoresis support is further a fifth metal oxide film for forming a fifth pH corresponding to a fifth isoelectric point of the sample, and is disposed in the fifth region, The electrophoresis support according to claim 2 or 3, comprising a fifth metal oxide film formed of a first metal oxide and the second metal oxide.
  7. 　前記第５の金属酸化膜は、
    　前記第１の金属酸化物で形成され、前記第５の領域において点在する複数の第１の金属酸化膜片と、
    　前記第２の金属酸化物で形成され、前記第５の領域において点在する複数の第２の金属酸化膜片と、を含み、
    　前記担体を平面視した際に、前記複数の第１の金属酸化膜片の各々は、前記複数の第２の金属酸化膜片の各々と互いに重ならないように配置されている
    　請求項６に記載の電気泳動支持体。     The fifth metal oxide film is
    A plurality of first metal oxide film pieces formed of the first metal oxide and scattered in the fifth region;
    And a plurality of second metal oxide film pieces formed of the second metal oxide and scattered in the fifth region;
    The plurality of first metal oxide film pieces are arranged so as not to overlap with each of the plurality of second metal oxide film pieces when the carrier is viewed in plan. Electrophoresis support.
  8. 　前記担体の前記表面には、さらに、前記第３の領域と前記第５の領域との間に配置された第６の領域が形成され、
    　前記電気泳動支持体は、さらに、前記第６の領域に配置され、前記試料の第６の等電点に対応する第６のｐＨを形成するための第６の金属酸化膜を備え、
    　前記第６の金属酸化膜は、
    　前記第１の金属酸化物で形成され、前記第６の領域において点在する複数の第３の金属酸化膜片と、
    　前記第２の金属酸化物で形成され、前記第６の領域において点在する複数の第４の金属酸化膜片と、を含み、
    　前記担体を平面視した際に、前記複数の第３の金属酸化膜片の各々は、前記複数の第４の金属酸化膜片の各々と互いに重ならないように配置されており、
    　前記複数の第１の金属酸化膜片と前記複数の第３の金属酸化膜片とは、面積及び数の少なくとも一方が異なり、且つ、前記複数の第２の金属酸化膜片と前記複数の第４の金属酸化膜片とは、面積及び数の少なくとも一方が異なる
    　請求項７に記載の電気泳動支持体。     Further, a sixth region disposed between the third region and the fifth region is formed on the surface of the carrier.
    The electrophoresis support further includes a sixth metal oxide film disposed in the sixth region to form a sixth pH corresponding to a sixth isoelectric point of the sample;
    The sixth metal oxide film is
    A plurality of third metal oxide film pieces formed of the first metal oxide and scattered in the sixth region;
    And a plurality of fourth metal oxide film pieces formed of the second metal oxide and scattered in the sixth region;
    Each of the plurality of third metal oxide film pieces is disposed so as not to overlap with each of the plurality of fourth metal oxide film pieces when the carrier is viewed in plan view,
    The plurality of first metal oxide film pieces and the plurality of third metal oxide film pieces differ in at least one of the area and the number, and the plurality of second metal oxide film pieces and the plurality of second metal oxide film pieces The electrophoresis support according to claim 7, wherein at least one of the area and the number of the metal oxide film pieces of No. 4 is different.
  9. 　前記第５の金属酸化膜は、
    　前記第１の金属酸化物で形成され、前記第５の領域において点在する複数の第１の金属酸化膜片と、
    　前記第２の金属酸化物で形成され、前記第５の領域において点在する複数の第２の金属酸化膜片と、を含み、
    　前記担体を平面視した際に、前記複数の第１の金属酸化膜片の各々は、前記複数の第２の金属酸化膜片の各々と互いに重なるように配置されている
    　請求項６に記載の電気泳動支持体。     The fifth metal oxide film is
    A plurality of first metal oxide film pieces formed of the first metal oxide and scattered in the fifth region;
    And a plurality of second metal oxide film pieces formed of the second metal oxide and scattered in the fifth region;
    The plurality of first metal oxide film pieces are disposed so as to overlap with each of the plurality of second metal oxide film pieces, when the carrier is viewed in plan. Electrophoresis support.
  10. 　前記第１の領域、前記第２の領域、前記第３の領域及び前記第４の領域の各区間長のうち少なくとも２つの区間長は、互いに異なる
    　請求項３に記載の電気泳動支持体。     The electrophoresis support according to claim 3, wherein at least two section lengths among the section lengths of the first area, the second area, the third area, and the fourth area are different from one another.
  11. 　試料を電気泳動するための電気泳動装置であって、
    　容器と、
    　前記容器の内部に配置された一対の電極と、
    　請求項１～１０のいずれか１項に記載の電気泳動支持体と、を備え、
    　前記一対の電極はそれぞれ、前記電気泳動支持体の前記試料の泳動方向における両端部に配置されている
    　電気泳動装置。     An electrophoresis apparatus for electrophoresis of a sample, wherein
    A container,
    A pair of electrodes disposed inside the container;
    An electrophoretic support according to any one of claims 1 to 10;
    An electrophoresis apparatus, wherein the pair of electrodes are respectively disposed at both ends of the electrophoresis support in the migration direction of the sample.
PCT/JP2018/037589 2017-10-27 2018-10-09 Electrophoretic support and electrophoresis device WO2019082640A1 (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005534914A (en) * 2002-07-31 2005-11-17 ソルヴィーアス　アクチェンゲゼルシャフト Apparatus and measuring method
US20060065528A1 (en) * 2004-02-03 2006-03-30 Gabriel Lopez Nanostructured devices for separation and analysis
US20120228142A1 (en) * 2004-03-31 2012-09-13 Scott Sibbett Fabrication and use of semipermeable membranes and gels for the control of electrolysis
WO2016163097A1 (en) * 2015-04-10 2016-10-13 パナソニックＩｐマネジメント株式会社 Electrophoretic support body and electophoretic device
WO2018030052A1 (en) * 2016-08-10 2018-02-15 パナソニックＩｐマネジメント株式会社 Electrophoresis support, electrophoresis device, and method for manufacturing electrophoresis support

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2005534914A (en) * 2002-07-31 2005-11-17 ソルヴィーアス　アクチェンゲゼルシャフト Apparatus and measuring method
US20060065528A1 (en) * 2004-02-03 2006-03-30 Gabriel Lopez Nanostructured devices for separation and analysis
US20120228142A1 (en) * 2004-03-31 2012-09-13 Scott Sibbett Fabrication and use of semipermeable membranes and gels for the control of electrolysis
WO2016163097A1 (en) * 2015-04-10 2016-10-13 パナソニックＩｐマネジメント株式会社 Electrophoretic support body and electophoretic device
WO2018030052A1 (en) * 2016-08-10 2018-02-15 パナソニックＩｐマネジメント株式会社 Electrophoresis support, electrophoresis device, and method for manufacturing electrophoresis support

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