WO2019050251A1

WO2019050251A1 - Device for reducing size of biological tissue

Info

Publication number: WO2019050251A1
Application number: PCT/KR2018/010278
Authority: WO
Inventors: 이준석
Original assignee: 이준석
Priority date: 2017-09-05
Filing date: 2018-09-04
Publication date: 2019-03-14

Abstract

A device for reducing the size of biological tissue according to an embodiment comprises: a plate; and a thru-hole which penetrates the front and the rear of the plate and is defined by a plurality of edges of the plate, wherein each of the plurality of edges comprises a protruding part protruding toward a center part of the thru-hole, and biological tissue may be reduced in size by being scratched and torn by the protruding parts while passing through the thru-hole.

Description

A biomedical tissue fining device

Hereinafter, embodiments are directed to a biomedical tissue fining apparatus.

Fat tissue, which is a type of living tissue, is obtained from a subject such as a person or an animal by suction or incision. Since the fat tissue thus obtained is large in size and contains a large amount of fibrous material, it is difficult to implant it into a subject using a fine needle. Accordingly, devices for reducing the size of living tissue, such as large-sized fat tissue, have been developed. For example, U.S. Patent No. 6,139,757 discloses a method of separating cells from blood using a variable porosity filter.

An object according to an embodiment is to provide a device for reducing the size of a living tissue in a scratch or tearing manner.

An object according to an embodiment is to provide an apparatus for gradually reducing the size of a living tissue.

According to one embodiment, a biomedical tissue refinement apparatus includes a plate; And a through hole penetrating the front and rear of the plate and defined by a plurality of rims of the plate, wherein the plurality of rims each include a protrusion protruding toward a central portion of the through hole, While passing through the hole, the biotissue can be scratched and torn by the protrusion, which can be reduced in size.

The projecting portion is narrowed toward the central portion of the through hole and can form a peak.

The plurality of rims may include linear portions formed on both sides with respect to the protruding portion. In other words, the protrusion can be formed at the center of the rim.

A first group of protrusions angled with respect to the plate and projecting in a first direction with respect to the plate; And a second group of protrusions projecting in a second direction different from the first direction at an angle relative to the plate and with respect to the plate. In other words, the first group of protrusions and the second group of protrusions can be formed in a plurality of rims in various ways. In a non-limiting example, the protrusions of the first group appear continuously along the rims of some of the plurality of rims, and the protrusions of the second group may appear consecutively along the remaining rims of the plurality of rims.

The first group of protrusions and the second group of protrusions may alternate in different directions along the plurality of rims.

The extension of each of the protrusions of the first group and the extension of each of the protrusions of the second group may not be in contact with each other nor may they be parallel to each other. In other words, the extension of the first direction and the extension of the second direction may be in a twisted position.

Wherein the biomedical tissue fining device comprises: an extension extending from each of the plurality of rims toward a central portion of the through hole; And a distal end portion formed at an end of the extended portion and configured to scratch and tear the living tissue.

The living tissue microfabrication apparatus may further include an additional extension formed on the opposite side of the extended portion with respect to the proximal end portion and extending toward the central portion of the through hole.

The projecting direction of the projecting portion may not be parallel to the moving direction of the living tissue passing through the through hole.

The apparatus includes a first plate, a first through-hole passing through the front and rear of the first plate and defined by a plurality of first rims of the first plate, A first projection including a first projection projecting toward a center of the first screen; And a second through hole penetrating the front and rear sides of the second plate and defined by a plurality of second rims of the second plate and a second through hole penetrating the front and rear sides of the second plate and defined by a plurality of second rims of the second plate, Wherein the first through hole and the second through hole overlap each other, and the size of the first through hole is larger than the size of the second through hole .

The projecting direction of the first projecting portion and the second projecting portion may be the same or intersect with each other without being parallel to the moving direction of the living tissue sequentially passing through the first through hole and the second through hole.

The biomedical tissue refining apparatus according to one embodiment can reduce the size of a living tissue by a scratch or a tear method.

The biomedical tissue refinement apparatus according to an embodiment can gradually reduce the size of a living tissue.

The effects of the biomedical tissue refining apparatus according to one embodiment are not limited to those mentioned above, and other effects not mentioned can be clearly understood by those skilled in the art from the following description.

FIG. 1 is a perspective view schematically showing a biomedical tissue refinement apparatus according to an embodiment of the present invention.

2 is an exploded side view schematically showing an apparatus for biomedical tissue refinement according to an embodiment.

3 is a cross-sectional view schematically showing a part of a biomedical tissue refinement apparatus according to an embodiment.

4 is a plan view schematically illustrating a screen according to an embodiment.

FIG. 5 is an enlarged view of an exemplary embodiment of the portion A of FIG. 4; FIG.

FIG. 6 is an enlarged view of another exemplary embodiment of FIG. 4A.

7 is a side view schematically illustrating a screen according to one embodiment.

FIG. 8 is an enlarged view of an exemplary embodiment of FIG. 7B.

9 is an enlarged cross-sectional view of one exemplary embodiment of a screen and a portion thereof in accordance with one embodiment.

10 is an enlarged cross-sectional view of another exemplary embodiment of a screen and a portion thereof in accordance with an embodiment.

11 is a plan view schematically illustrating an example of a plurality of stacked screens according to an embodiment.

12 is a sectional view taken along line 12-12 in Fig.

13 is a plan view schematically illustrating another example of a plurality of stacked screens according to an embodiment.

14 is a sectional view taken along line 14-14 of Fig.

Hereinafter, embodiments will be described in detail with reference to exemplary drawings. It should be noted that, in adding reference numerals to the constituent elements of the drawings, the same constituent elements are denoted by the same reference numerals even though they are shown in different drawings. In the following description of the embodiments, detailed description of known functions and configurations incorporated herein will be omitted when it may make the best of an understanding clear.

In describing the components of the embodiment, terms such as first, second, A, B, (a), and (b) may be used. These terms are intended to distinguish the constituent elements from other constituent elements, and the terms do not limit the nature, order or order of the constituent elements. When a component is described as being "connected", "coupled", or "connected" to another component, the component may be directly connected or connected to the other component, Quot; may be " connected, " " coupled, " or " connected. &Quot;

The components included in any one embodiment and the components including common functions will be described using the same names in other embodiments. Unless otherwise stated, the description of any one embodiment may be applied to other embodiments, and a detailed description thereof will be omitted in the overlapping scope.

FIG. 1 is a perspective view schematically showing a biomedical tissue refinement apparatus according to an embodiment, FIG. 2 is an exploded side view schematically showing a biomedical tissue refinement apparatus according to an embodiment, FIG. 3 is a cross- Figure 2 is a cross-sectional view schematically showing a part of the device.

Referring to FIGS. 1 and 2, the biomedical tissue refinement apparatus 1 according to one embodiment is configured to maximize contact with a living tissue and to miniaturize a living tissue to obtain a biomolecule of a desired size. For example, the biological tissue may include adipose tissue. The living tissue can be obtained from a subject such as a human being, an animal, or the like, in a manner such as suction or incision.

The biomedical tissue refinement apparatus 1 may include a screen 10, a sealing member 12, a front cover 14, and a rear cover 16.

The screen 10 is configured to reduce the size of the living tissue. For example, the screen 10 may have a disk shape. While the lumpy living tissue passes through the screen 10, the screen 10 may be configured to scratch or tear the lumpy living tissue.

The sealing member 12 is configured to surround the outside of the screen 10. The sealing member 12 may form a seal between the screen 10 and the front cover 14 and the rear cover 16. [ The sealing member 12 may have an annular shape. For example, the sealing member 12 may be formed of a rubber material.

The front cover 14 and the rear cover 16 are configured to receive the screen 10 and the sealing member 12, respectively. The front cover 14 and the rear cover 16 can be coupled to each other. For example, the front cover 14 and the rear cover 16 may be rotationally coupled.

The front cover 14 and the rear cover 16 may each have an inlet port and an outlet port through which the living tissue flows and a passage connecting the inlet port and the outlet port is formed between the front cover 14 and the rear cover 16 . The biotissue or mass thereof can enter the interior of the front cover 14 through the inlet and pass out of the interior of the rear cover 16 through the outlet after passing through the screen 10 along the passageway.

Referring to FIG. 4, the screen 10 may include a plate 110 and at least one through hole 120.

The plate 110 may have a disc shape. For example, the plate 110 may be circular. The plate 110 may be formed of a material suitable for preventing contamination of living tissue.

The through hole 120 is configured to maximize the contact with the living tissue and make the living tissue finer. The through holes 120 pass through the front and rear of the plate 110 and are defined by a plurality of rims of the plate 110. The plurality of rims of the plate 110 defining the through holes 120 may have irregular dimensions.

The plate 110 may be entirely plate molded so that the position of the through hole 120 is changed in the plate 110 or the shape of the through hole 120 is not deformed by an external force.

Referring to FIG. 5, a plurality of rims 111 defining the through holes 120 may define the through holes 120 so that the through holes 120 have a substantially rectangular shape.

The plurality of rims 111 may each include a protrusion 112. The protrusion 112 is configured to protrude toward the center of the through hole 120. As the biotissue passes through the through hole 120, the protrusion 112 is configured to scratch or tear the biotissue. On the other hand, fibers larger than the through-holes 120 may not pass through the through-holes 120.

The protruding direction of the protruding portion 112 may not be parallel to the moving direction of the living tissue passing through the through hole 120. In one example, the projecting direction of the protrusion 112 may be parallel to the tangential direction of the plate 110, or may be a set angle with respect to the tangential direction of the plate 110. Here, the angle formed by the protrusion 112 with respect to the plate 110 may be set to an acute angle, an obtuse angle, or a reflex angle.

The projecting portion 112 is narrowed toward the central portion of the through hole 120 and can form a tip 1121. For example, the protrusion 112 may have the shape of a triangle.

The plurality of rims 111 may include

linear portions

114 and 116 formed on both sides with respect to the protruding portion 112. In other words, the protrusion 112 may be located at the center of the rim 111 thereof. In one example, the

linear portions

114, 116 may be the same length so that the protrusions 112 are at the center of the rim 111. In this case, the protrusions 112 of one of the rims 111 and the protrusions of the opposite rims may face each other toward the center of the through hole 120. In the example not shown, the lengths of the

linear portions

114 and 116 may be set differently. The length of the

linear portions

114, 116 can be adjusted in consideration of the size of the protrusion 112.

The irregular shape of the through hole 120 defined by the plurality of rims 111 as described above and the sharp cross section of the plurality of rims 111 cause a pressure applied to the living tissue passing through the through hole 120 And the lumpy biotissue may be scratched or torn.

FIG. 6 is an enlarged view of another exemplary embodiment of FIG. 4A.

Referring to FIG. 6, a plurality of rims 111 'defining the through holes 120 may be defined by defining the through holes 120 so that the through holes 120 have polygons having substantially four or more corners. can do. The plurality of rims 111 'may include a protrusion 112' configured to protrude toward the center of the through hole 120. The protrusion 112 'is narrowed toward the central portion of the through hole 120 and can form a tip 1121.

In this embodiment, the plurality of rims 111 'are formed such that the linear portions formed on both sides with respect to the protruding portion 112' are not present, or even if they are present, the length is relatively large compared to the size of the protruding portion 112 ' It can be short. In other words, the plurality of protrusions 112 'may be adjacent to each other along the plurality of rims 111'.

FIG. 7 is a side view schematically illustrating a screen according to an embodiment, and FIG. 8 is an enlarged view of an exemplary embodiment of FIG. 7B.

7 and 8, a screen 20 according to an embodiment may include a plate 210 and a through hole 220. [ The

protrusions

212a and 212b of the plurality of

rims

211a and 211b of the plate 210 may establish a set angle with respect to the tangential direction of the plate 210. In this embodiment, The moving direction of the lumen-like living tissue BM passing through the through hole 220 at the front and rear of the screen 20 may not be parallel to the protruding direction of the

protrusions

212a and 212b. In other words, the

protrusions

212a and 212b may be configured to refine the living tissue BM by scratching or tearing the living tissue BM rather than slicing the living tissue BM.

The plurality of

rims

211a and 211b may include

protrusions

212a and 212b having at least one directionality with respect to the tangential direction of the plate 210. [ In other words, the plurality of

protrusions

212a and 212b may be divided into a plurality of groups according to their protruding directions. For example, the plurality of protrusions 212a, 212b may be angled relative to the plate 210 to define a first group of protrusions protruding in a first direction with respect to the plate 210, And a second group of protrusions protruding in a second direction with respect to the plate 210.

Here, the first direction and the second direction may be different from each other. The fact that the first direction and the second direction are different from each other means that they are not parallel to each other. For example, the protrusion 212a in the first direction protrudes to the left of the plate 210 with reference to Fig. 8, and the protrusion 212b in the second direction protrudes to the right of the plate 210 with reference to Fig. can do.

In one example, the first group of protrusions 212a and the second group of protrusions 212b may be adjacent to each other. In a preferred example, the first group of protrusions 212a and the second group of protrusions 212b may alternate with each other.

In the example not shown, the plurality of protrusions 212a of the first group and the plurality of protrusions 212b of the second group are grouped and may be adjacent within each group. In this case, the first group of protrusions 212a may be continuously formed on the rims of some of the plurality of

rims

211a and 211b, and the second group of protrusions 212b may be continuously formed on the remaining rims have.

As described above, the arrangements of the first group of protrusions 212a and the second group of protrusions 212b may be various.

In one embodiment, the extension of the first group of protrusions 212a and the extension of the second group of protrusions 212b may or may not be parallel to each other. In other words, the extension of the first group of protrusions 212a and the extension of the second group of protrusions 212b may be at a skew position in the three-dimensional space. For example, the vertex at which the rim 211a including the first group of protrusions 212a and the rim 211b including the second group of protrusions 212b meet as the origin, The first direction being the direction in which the protrusions 212a protrude is defined as the x axis, the second direction in which the second group of protrusions 212b protrude is defined as the y axis, the outward in the first direction and the second direction is defined as the z axis The vector from the rim 212a including the first group of protrusions 212a to the tip of the protrusion 212a is (1, 0, -1), and the second group of protrusions 212b The vector from the containing edge 212b to the tip of the protrusion 212b may be (-1, 0, 1).

Referring to FIG. 9, a screen 30 according to one embodiment may include a plate 310 and a through hole 320. In this embodiment, a plurality of rims 311 of the plate 310 defining the through holes 320 may be formed with a sharp cross section over the entire plurality of rims 311. For example, the screen 30 is formed at the distal end of the extending portion 314 and the extending portion 314 extending from each of the plurality of rims 311 toward the central portion of the through hole 320, (Not shown).

The extension 314 may have any suitable shape. In one example, the extension 314 may be formed obliquely with respect to the plane of the plate 310. In another example, the extension 314 may have an outwardly curved shape with respect to the plate 310.

Referring to FIG. 10, a screen 40 according to one embodiment may include a plate 410 and a through hole 420. In this embodiment, a plurality of

extensions

414 and 415 may be formed on both sides of the rim 411 defining the through hole 420 with respect to the tip 416. [ In one example, the plurality of

extensions

414, 415 may be formed in a plurality of rims 411 at an angle to the plate 410 at respective set angles. In a preferred example, the inclination angles of the plurality of

extensions

414, 415 relative to the plate 410 may be substantially the same.

In a further embodiment, the plurality of

extensions

414, 415 may have an outwardly curved shape with respect to the plate 310. In this case, the curved directions of the plurality of extending

portions

414 and 415 may be different from each other. In one example, the radius of curvature of the plurality of

extensions

414, 415 may be substantially the same so that the tip 416 is centered between the plurality of

extensions

414, 415.

FIG. 11 is a plan view schematically showing an example of a plurality of stacked screens according to an embodiment, and FIG. 12 is a sectional view taken along line 12-12 in FIG.

Referring to FIGS. 11 and 12, the biomedical tissue refinement apparatus 5 according to an embodiment is configured such that a plurality of

screens

50a, 50b, and 50c are sequentially laminated. The plurality of

screens

50a, 50b and 50c may include through

holes

520a, 520b and 520c defined by

plates

510a, 510b and 510c and a plurality of

rims

511a, 511b and 511c, respectively. have. The plurality of

screens

50a, 50b and 50c may include

projections

512a, 512b and 512c projecting toward the respective through

holes

520a, 520b and 520c. As described above with reference to FIG. 9, one extended portion and a leading end portion may be formed on the plurality of

frames

511a, 511b, and 511c.

In this embodiment, the through-

holes

520a, 520b, and 520c of the plurality of

screens

50a, 50b, and 50c that are stacked one after another in the direction of decreasing the size (from top to bottom with reference to FIG. 12) They can overlap each other. Accordingly, the living tissue passing through the plurality of

screens

50a, 50b, and 50c may have a sharp cross section formed on the plurality of

rims

511a, 511b, and 511c and / or a shape of the

protrusions

512a, 512b, and 512c And can be gradually refined.

FIG. 13 is a plan view schematically showing another example of a plurality of stacked screens according to an embodiment, and FIG. 14 is a sectional view taken along line 14-14 of FIG.

Referring to FIGS. 13 and 14, the biomedical tissue refinement apparatus 6 according to an embodiment may include a plurality of

screens

60a, 60b, and 60c. The plurality of

screens

60a, 60b and 60c are respectively provided with through

holes

620a, 620b and 620c defined by the

plates

610a, 610b and 610c, a plurality of

rims

611a, 611b and 611c and

protrusions

612a , 612b, 612c. As described above with reference to FIG. 10, a plurality of extensions may be formed on both sides of the plurality of

rims

611a, 611b, and 611c on the basis of one prong.

Similarly, in this embodiment, the through-

holes

620a, 620b, and 620c of the plurality of

screens

60a, 60b, and 60c that are stacked in order are arranged in a direction Respectively.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. For example, it is to be understood that the techniques described may be performed in a different order than the described methods, and / or that components of the described systems, structures, devices, circuits, Lt; / RTI > or equivalents, even if it is replaced or replaced.

Claims

plate; And

A through hole passing through the front and rear of the plate and defined by a plurality of edges of the plate;

/ RTI >

The plurality of rims each include a protrusion protruding toward the central portion of the through hole,

Wherein the biotissue is scratched and torn by the protruding portion while the biotissue passes through the through hole, and the size of the biotissue is reduced.
The method according to claim 1,

Wherein the projecting portion is narrowed toward the central portion of the through hole and forms a tip.
The method according to claim 1,

Wherein the plurality of rims each include linear portions formed on both sides with respect to the protruding portion.
The method according to claim 1,

A first group of protrusions angled with respect to the plate and projecting in a first direction with respect to the plate; And

A second group of protrusions angled with respect to the plate and projecting in a second direction different from the first direction with respect to the plate;

And a biochemical microfabrication apparatus.
5. The method of claim 4,

Wherein the protrusions of the first group and the protrusions of the second group alternate in different directions along the plurality of rims.
5. The method of claim 4,

Wherein an extension of each of the protrusions of the first group and an extension of each of the protrusions of the second group do not meet with each other and are not parallel to each other.
The method according to claim 1,

An extension extending from each of the plurality of rims toward a central portion of the through hole; And

A tip portion formed at an end of the extended portion and configured to scratch and tear the living tissue;

Further comprising a biochemical microfabricator.
8. The method of claim 7,

Further comprising an additional extension formed on the opposite side of the proximal portion relative to the proximal portion and extending toward the center of the through-hole.
The method according to claim 1,

And the projecting direction of the projecting portion is not parallel to the moving direction of the living tissue passing through the through hole.
A first through hole penetrating the front and rear sides of the first plate and defined by a plurality of first rims of the first plate and a first protrusion protruding toward the center of the first through hole, A first screen comprising; And

A second through hole penetrating the front and rear sides of the second plate and defined by a plurality of second rims of the second plate and a second through hole defined by a plurality of second rims of the second plate, A second projection including a second projection projecting toward the second projection;

/ RTI >

Wherein the first through hole overlaps with the second through hole and the size of the first through hole is larger than the size of the second through hole.
11. The method of claim 10,

Wherein the projecting directions of the first projecting portion and the second projecting portion are the same or intersect with each other without being parallel to the moving direction of the living tissue sequentially passing through the first through hole and the second through hole.