WO2018076233A1

WO2018076233A1 - Menstrual blood detection device

Info

Publication number: WO2018076233A1
Application number: PCT/CN2016/103549
Authority: WO
Inventors: 李鹏
Original assignee: 广州悦鸿方生物科技有限公司
Priority date: 2016-10-27
Filing date: 2016-10-27
Publication date: 2018-05-03

Abstract

A menstrual blood detection device suitable for a tampon (5), the device including a menstrual blood collection system and a detection system connected to the menstrual blood collection system. The detection system includes a sensor, a wireless signal transmission device (14) and a power supply device (15). Menstrual blood (6) in the tampon (5) enters the detection system by means of a filtration device (7) after being desorbed in the menstrual blood collection system. The sensor in the detection system converts physical, chemical and biological signals detected in the menstrual blood (6) into electrical signals, and then, the wireless signal transmission device (14) sends data of the sensor to electronic equipment (17), and the electronic equipment (17) obtains health and physiological indexes of a female by means of further analysis and processing.

Description

Blood transfusion detecting device

Technical field

The invention relates to a blood transfusion detecting device, in particular to a detecting device suitable for tampon and for measuring physiological and biochemical indexes in menstrual blood.

Background technique

Sampling and diagnosis of gynecological disease detection is damaging, time lag and dependent on hospitals and health care providers. For example, in the process of biochemical and biological testing, blood is taken for damage, blood components, various detection markers, cells, etc. are used for diagnosis. In pathological examination, invasive biopsy is performed on cancer tissues. And detection and analysis of cell levels with or without abnormalities. In addition, the detection of urine, feces, saliva, etc., is limited by the sampling location, test location and inspectors.

This is especially true for sampling and diagnosis of gynecologic tumor testing. However, female-specific high-grade tumors, such as ovarian cancer, uterine cancer, cervical cancer and breast cancer, have been a deadly disease that harms women's health and life worldwide because of their delayed diagnosis and high mortality rate; therefore, Early prediction and diagnosis of diseases including gynecological tumors is of great significance for the maintenance and improvement of women's health and quality of life. However, due to the shame and unspeakableness of women's diseases, patients go to the hospital until they are seriously ill. At this time, the best treatment time for the disease may have been missed.

Although the diagnosis of some gynecologic tumors is not damaging, such as the detection of cervical cancer, HPV (human papillomavirus) detection and Pap smear, however, both of these tests require patients to collect samples from the clinic, and Certainly invasive, causing discomfort to the patient.

Therefore, it is of great significance to develop a non-invasive, invasive, time-dependent, site-dependent, and detector-dependent detection device that can diagnose diseases in advance, especially gynecological tumors.

Menstruation is the periodic detachment of the endometrium with bleeding. The main component of menstrual blood is blood. In addition, it contains fragments of endometrium, cervical mucus, vaginal epithelial cells, various active enzymes and biological factors. Therefore, menstrual blood is an ideal sample for tumor markers or other disease diagnosis.

Tampons can be placed in the vagina to absorb menstrual blood when women have menstrual cramps. Because they are dry and comfortable, they are not easy to leak, and super suction is popular abroad. The tampon has a larger amount of menstrual blood absorption than the sanitary napkin and is smaller in size. In addition, the tampon is placed in the vagina to absorb menstrual blood and has a certain isolation from the external environment. Therefore, the tampon is more suitable for collecting and analyzing menstrual blood. a.

However, so far, in clinical or research, there is no device or product used to detect physiological and biochemical indicators in menstrual blood, and there is no device or product that can be directly used to collect menstrual blood in tampon or directly In the menstrual blood Analysis of physiological and biochemical indicators to diagnose and predict women's health and physical status.

Summary of the invention

One of the objects of the present invention is to provide a menstrual blood collection system suitable for a tampon, which collects the menstrual blood in the tampon, and further analyzes the physiological and biochemical indexes in the menstrual blood through third-party related instruments and technical means, thereby diagnosing the disease. Occurrence, check the physiological status of women.

The second objective of the present invention is to provide a menstrual blood detecting device suitable for a tampon. After collecting the menstrual blood in the tampon, the physiological and biochemical indicators in the menstrual blood can be detected in situ in real time and passed through a wireless signal. The test results are fed back to the electronic device.

The object of the invention is achieved by the following technical means:

The present invention provides a menstrual blood collection system.

The menstrual blood collection system includes a cylindrical first cavity and a second cavity below the first cavity.

The first cavity is a tampon storage cavity. The first cavity is a space defined by a first cavity wall of a cylindrical shape, an elliptical cylinder shape, a prismatic shape or an irregular shape, and a partition plate located below the first cavity.

The shape of the first cavity wall is preferably cylindrical; as a preferred embodiment, the inner diameter of the first cavity wall is not less than the outer diameter of the tampon, and can be used for loading the tampon; more preferably, the inner diameter of the cavity wall is not less than 2 cm; more preferably, the inner diameter of the chamber wall is not less than 4 cm.

The separator is a perforated separator.

The lower portion of the partition is a second cavity defined by the partition and the second cavity wall below the partition; preferably, a bottom plate is disposed below the second cavity wall;

Optionally, the first cavity wall and the second cavity wall are hollow or non-hollowed;

Preferably, a filter device is disposed above the perforated partition; preferably, the filter device is a filter membrane, a filter, a filter, a filter gel or a filter column; more preferably a filter membrane.

A cover is disposed above the first cavity.

The first cavity is further provided with a pressure difference mechanism. The pressure difference mechanism desorbs the menstrual blood in the tampon by compressing the volume of the space of the first cavity.

The pressure difference mechanism is a pressure applying device, further, a piston or a diaphragm; the pressing device is located under the cover or the first cavity wall. For example, the pressing device is a device with a spring under the cover, and when the cover is pressed, the volume of the cavity of the first cavity is simultaneously compressed to desorb the menstrual blood in the tampon. Alternatively, the pressure applying device is located on the first chamber wall, so that the volume of the cavity of the first cavity is contracted, the pressure in the cavity is increased, and the menstrual blood in the tampon is desorbed.

Alternatively, the pressure difference mechanism is a vacuum device, further a vacuum pump, a piston that can be stretched, or a diaphragm.

As a preferred embodiment, when the amount of menstrual blood in the tampon is small, the tampon can be placed during use. Thereafter, a buffer is further added to precipitate the menstrual blood in the tampon into the buffer.

As a preferred embodiment, the menstrual blood collection system is a disposable component to prevent contamination between different menstrual blood samples.

Alternatively, the menstrual blood collection system is a recyclable component in addition to the filtration device. The filter device can be replaced by a cover opening above the first cavity; or, at the cavity where the filter device is located, a filter device replacement port is provided.

The second cavity is located below the perforated baffle and the two cavities are connected by threads or other fitting structures. The second chamber can be pre-loaded with a buffer protection solution.

When collecting menstrual blood, the tampon is placed in the first cavity, covered, and desorbed by blood under the action of a pressure difference mechanism; the separated menstrual blood passes through the filtering device and the perforated partition, enters the second cavity, and then The second cavity and the first cavity are separated, and the second cavity is sealed by a sealing cover or other sealing device, or directly connected to a third-party instrument for detection. Through third-party related instruments and technical means, the physiological and biochemical indicators in women's menstrual blood are further analyzed to diagnose the occurrence of diseases and check the physiological status of women.

The invention also provides a menstrual blood detecting device, characterized in that it comprises a first cavity, a second cavity below the first cavity, and a second cavity containing a detection system.

The first cavity is a tampon storage cavity.

The first cavity is a space defined by a first cavity wall of a cylindrical shape, an elliptical cylinder shape, a prismatic shape or an irregular shape, and a partition plate located below the first cavity; the first cavity wall The shape is preferably cylindrical;

Preferably, the inner diameter of the chamber wall is not less than the outer diameter of the tampon; more preferably, the inner diameter of the chamber wall is not less than 2 cm; more preferably, the inner diameter of the chamber wall is not less than 4 cm;

a cover is arranged above the first cavity,

The separator is a perforated separator;

The menstrual blood collection system is also provided with a pressure difference mechanism. The pressure difference mechanism desorbs the menstrual blood in the tampon by compressing the volume of the space of the first cavity.

The pressure difference mechanism is a pressure applying device, further, a piston or a diaphragm; the pressing device is located under the cover or the first cavity wall. For example, the pressing device is a piston located under the cover, and when the cover is pressed, the volume of the cavity of the first cavity is simultaneously compressed to desorb the menstrual blood in the tampon. Alternatively, the pressing device is located on the first cavity wall, so that the volume of the cavity of the first cavity is contracted, the pressure in the cavity is increased, and the menstrual blood in the tampon is desorbed through the filtering device and The hole partition enters the detection system.

As a preferred embodiment, when the amount of menstrual blood in the tampon is small, the tampon can be placed in the use, and then a buffer is added to precipitate the menstrual blood in the tampon into the buffer.

As a preferred embodiment, the menstrual blood collection system is a single use component;

The detection system includes one or more of a physical detection device, a chemical detection device, and a biological detection device.

Further, the detection system includes a sensor, a wireless signal transmission device, and a power supply device. After the blood contacts the sensor, the sensor converts the biological, chemical or physical signals in the blood into electrical signals, and then transmits the signals collected and converted by the sensor to the relevant software on the electronic device through the wireless signal transmission device for analysis and processing, and finally obtains the detection result. .

Preferably, the sensor is a MEMS sensor;

MEMS is a Micro-ElectroMechanical System. Micro-electro-mechanical systems are micro-devices that can be mass-produced, integrating micro-mechanisms, micro-sensors, micro-actuators, and signal processing and control circuits, up to interfaces, communications, and power supplies. Or system.

Preferably, the MEMS sensor is one or more of a MEMS physical sensor, a MEMS chemical sensor, and a MEMS biosensor.

Or preferably, the MEMS sensor is a microfluidic chip. Microfluidics Chip integrates basic operation units such as sample preparation, reaction, separation and detection in biological, chemical and medical analysis processes onto a micrometer-scale chip. The microchannel forms a network to control microfluidic penetration. The entire system automatically completes the analysis process.

As an example of an alternative microfluidic chip, researchers such as the University of Southern California use a heterogeneously integrated thin-film micro-vertical surface-emitting laser (VCSEL) and silicon photodiode (Si-PD) design. A packaged soft microfluidic fluorescence sensor.

Preferably, one or a combination of the sensor physical sensor, chemical sensor and biosensor.

The physical sensor is one or more of a humidity sensor, a temperature sensor, a thermal sensor, a weight sensor, and a volume sensor;

The chemical sensor is an ion sensitive sensor, a gas sensor, and a pH sensor;

The biosensor is one or more of a microbial sensor, an immunosensor, a tissue sensor, a cell sensor, an enzyme sensor, a nucleic acid sensor, and a protein sensor.

By setting different sensors in the detection system, various physiological and biochemical indicators in the menstrual blood can be detected. The physiological and biochemical indicators tested include, but are not limited to, tissue cells in the menstrual blood, microorganisms such as bacteria, fungi and viruses, proteins such as swollen One or more of tumor markers, HPV antigens and enzymes, trace elements, pH, hormones, menstrual blood volume, and the like.

As an embodiment, the invention detects that the physiological and biochemical indicators are one or more of tumor markers, such as C12 tumor markers, and the C12 tumor markers are specifically alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA). , neuron-specific enolase (NSE), glycogen 125 (CA125), glycogen 153 (CA153), glycogen 242 (CA242), glycogen 199 (CA199), prostate specific antigen (PSA), free prostate Specific antigen (f-PSA), ferritin (FER), β-human chorionic gonadotropin (β-HCG), human growth hormone (HGH).

As an embodiment, the physiological and biochemical indicators detected by the invention are one or several of the six sex hormones. The six hormones are specifically FSH (follicle stimulating hormone), LH (luteinizing hormone), PRL (prolactin), E2 (estradiol), PROG (progesterone), T (testosterone).

As an embodiment, the physiological and biochemical indicators detected by the present invention are one or several of the five thyroid gland, specifically triiodothyronine (FT3), free thyroxine (FT4), and thyroid stimulating hormone (TSH). Thyroid peroxidase antibody (TPOAb), thyroglobulin antibody (TGAb).

As an embodiment, the physiological and biochemical indicators detected by the invention are six pituitary bodies.

The sensor can be a sensor in the prior art or a sensor designed by itself.

The sensors in the prior art are the enzyme sensors, immunosensors, bacteria which have been commercialized or experimentally reported as reported in the document "Ren Shu. Biosensors and Sensors [J]. Instrument Technology and Sensors, 1987, 3:00. Sensors and other biosensors. Further examples are the multi-purpose enzyme sensor of Provesta corp. in the United Kingdom; the B.O.D sensor of Japan Rouge; the fluorescence polarization immunosensor of Abbot Laboratories (determination of total estriol in plasma).

Preferably, the sensor is a protein sensor. The protein sensor contains a protein chip.

Protein chip is a kind of immobilized probe protein (which can be antigen, according to a pre-designed method on the surface of solid support (filter, gel, slide, nanobead, microplate, polymer material, etc.). Antibodies, receptors, ligands, enzymes, substrates, etc.). The protein molecule with a special label (such as a fluorescent dye label) is incubated with the chip, and the probe can bind to and bind to the test protein (such as tumor marker, HPV antigen, etc.) in the sample, and then mark the label. The object is tested.

There are many types of protein chips to choose from, such as nano-array immunochips, liquid chips, surface-enhanced laser desorption/ionization chips, surface plasmon resonance detection chips, isotope-labeled detection chips, fluorescent label detection chips, chemiluminescence detection chips, Enzyme immunolabeling detection chip, colloidal gold labeling detection chip, optical interference film chip, and the like.

As an illustrative example, the protein chip of the present invention is a chip for chemiluminescence detection.

An exemplary detection principle of a chemiluminescence detection chip is shown in Figure 6: an antibody that binds to horseradish peroxidase or alkaline phosphatase, an enzyme that binds to a luminescent agent, catalyzes and decomposes a luminescent agent to produce an extended diffuse ray, read by photon The system receives, and the photo-electrical tube converts the optical signal into an electrical signal and amplifies it.

Chemiluminescence has the specificity of fluorescence, and does not require excitation light, avoids the influence of excitation light and stray light in fluorescence analysis, and has high sensitivity.

As a preferred embodiment, the protein sensor of the present invention contains a chemiluminescent detection protein chip and a photoelectric converter and signal amplifier.

As an illustrative example, the protein chip of the present invention is a fluorescent label detection chip.

The exemplary detection principle of the fluorescent label detection chip is shown in Fig. 7: the solid phase antibody-fluorescent labeling antibody in the blood-contacting antigen in the contact chip forms a "solid phase antibody-test antigen-fluorescent label antibody" sandwich structure. The immune complex, under the illumination of the excitation light, the fluorescently labeled antibody emits fluorescence, and then the fluorescent signal is received and converted into an electrical signal and amplified, and then the electrical signal is analyzed. The fluorescently labeled protein chip is shown in Figure 3 and comprises a substrate or a sample cell. The substrate is a transparent material or an opaque material. When the excitation light source is located above the protein chip, the substrate is transparent or opaque; when the excitation light source is located under the protein chip, the substrate is transparent. The material, such as quartz glass, optical glass, optical plastic, etc., which can transmit fluorescence and visible light, or the substrate is a permeable filter, and the blood can penetrate the side of the substrate close to the photoelectric converter for reaction. The sample cell is equipped with an agent that specifically reacts with the substance to be tested.

As a preferred embodiment, the protein sensor of the present invention comprises a fluorescent label-detected protein chip, an excitation light source, a photoelectric converter, and a signal amplifier.

The substance in the blood is specifically combined with the substance in the protein chip to emit an optical signal under the action of the excitation light, and the optical signal is converted into an electrical signal by the photoelectric converter and the signal amplifier and amplified, and then the electrical signal is transmitted through the wireless signal transmission device. To external electronic devices.

In a preferred embodiment of the invention, the excitation light source is located above the protein chip and on the inner wall of the second cavity. On the outer wall of the second chamber, a power switch is also provided for controlling the switching of the power source. The switch may be disposed at any position on the outer wall of the second chamber, such as a switch disposed on the outer wall of the chamber corresponding to the excitation light source. In this case, the signal amplifier and the photoelectric converter are located above the protein chip.

In another preferred embodiment of the invention, the excitation light source is located below the protein chip, on the inner wall of the second cavity. On the outer wall of the second chamber, a power switch is also provided for controlling the switching of the power source. The switch may be disposed at any position on the outer wall of the second chamber, such as a switch disposed on the outer wall of the chamber corresponding to the excitation light source. In this case, the signal amplifier and the photoelectric converter are located below the protein chip; and the substrate of the protein chip is transparent, and the emitted light and the excitation light can pass through the bottom of the sample cell.

As a preferred embodiment, the protein chip is a single use component.

As a preferred embodiment, the excitation light source is also connected with an automatic delay closing device, and the light source is automatically turned off when the excitation light source is turned on for a certain period of time.

The wireless signal transmission device is a WiFi chip, a Bluetooth chip or an RFID chip.

The power supply device is a lithium battery, a rechargeable battery, a dry battery, etc. Preferably, a power supply device replacement port is disposed under the second cavity. The power supply device provides power to the sensor or the pressure difference mechanism. The power supply device is located below the second cavity. The excitation light source, the signal transmitter, the photoelectric converter, and the pressure difference mechanism may share one power supply device or separately Different power supply devices. Preferably, a power supply device is shared to save the volume of the cavity.

A system for detecting proteins in the menstrual blood such as tumor markers and HPV antigens, wherein the operation flow is to open the lid above the first cavity, put the tampon into the first cavity, cover the lid, and then pass the pressure difference mechanism The menstrual blood in the tampon is analyzed, and the analyzed menstrual blood passes through a filtering device and a perforated partition to enter the detection system of the second cavity.

In the detection system, a protein to be tested, such as an antigen, in the blood is combined with a ligand and a fluorescent substance immobilized on the substrate. The fluorescent substance emits fluorescence, the fluorescent light is converted into an electrical signal by the photoelectric converter, and then amplified by the signal amplifier, and the wireless signal transmitting device transmits the amplified electrical signal to the external electronic device. After receiving the signal, the intelligent electronic device analyzes and processes the signal through relevant software, and finally returns the detection result to the user.

The beneficial effects obtained by the invention:

1) The present invention provides for the first time a menstrual blood collection system that can be used to collect menstrual blood in a tampon, which is simple in construction, convenient to use, and free from pollution.

2) The present invention provides for the first time a blood transfusion detecting device which is convenient for sampling and diagnosis: no need for the patient to appear in the clinic, and the patient is not humiliated, non-invasive and invasive. There is no commercial device worldwide that can detect female diseases in menstrual blood, especially tumor markers. The present invention is a disease for women, such as specific high-risk tumors (such as ovarian cancer, uterine cancer, cervical cancer and breast cancer). The possibility is provided, which is of great significance for the maintenance and improvement of women's health and quality of life. In addition, women's disease detection can be put into daily use, reduce the medical cost of the whole society, and greatly improve the health of women.

3) Real-time diagnosis: The detection device of the invention integrates sample collection, detection and data processing and processing, and only needs to use the device of the invention during menstruation, and can receive diagnostic data through the electronic device without sending the blood sample to the hospital for analysis. .

4) Multiple indicators can be detected at the same time: a) Different physiological and biochemical indicators can be detected by setting different sensors, such as menstrual blood volume, pH, microorganisms, etc. b) For blood transfusion detecting devices containing protein sensors, By adjusting the ligand on the substrate, different diseases are diagnosed, such as simultaneous detection of five indicators of hepatitis B, detection of tumor markers, detection of trace antigens and antibodies, identification of SARS antibody drugs, detection of viruses, and detection of acute cardiac infarction diagnostic markers.

5) The device of the present invention is completely sealed, and is not contaminated by the external environment during the detection process.

6) In addition to being applied to a tampon, the menstrual blood detecting device of the present invention can also be applied to a menstrual cup.

DRAWINGS

1 is a schematic view of a menstrual blood collection system in Embodiment 1 of the present invention;

2 is a schematic view of a menstrual blood detecting device in Embodiment 2 of the present invention;

Figure 3 is a schematic diagram of a protein chip

Figure 4 is a schematic view of a menstrual blood detecting device in Embodiment 3 of the present invention;

Figure 5 is a schematic view of a menstrual blood detecting device in Embodiment 4 of the present invention;

Figure 6 is a schematic diagram of the principle of chemiluminescence detection

Figure 7 is a schematic diagram of the principle of detection of a fluorescent label

Schematic description:

1. First cavity 2. Second cavity

3. Cover 4. Pressure difference mechanism

5. Tampons 6. Menstrual blood

7. Filter device 8. Perforated partition

9. Protein chip 10. Excitation light source

11. Excitation light switch 12. Lens

13. Photoelectric converter and signal amplifier 14. Wireless signal transmission device

15. Power supply unit 16. Battery replacement port

17. Electronic Devices 18. Dichroic Filters

19. Substrate 20. Sample Cell

21. First baffle 22. Second baffle

23. First cavity wall 24. Second cavity wall

25. Base plate

detailed description

The technical solutions of the present invention are further illustrated by the following specific examples, which are not intended to limit the scope of the present invention. Some non-essential modifications and adaptations made by others in accordance with the teachings of the present invention are still within the scope of the present invention.

Note: The lines with arrows in the drawing indicate the light path.

Example 1 A blood collection system

The menstrual blood collection system as described in Fig. 1 comprises a cylindrical first cavity 1 and a second cavity 2 located below the first cavity 1.

The first cavity 1 is for placing a tampon 5 that absorbs menstrual blood 6. The first cavity 1 contains a first cavity wall 23. A cover 3 is disposed above the first cavity 1. The first chamber 1 is further provided with a pressure difference mechanism 4, which in the present embodiment is a pressure applying device; further, a piston located below the cover. By automatically or manually squeezing the piston, the cavity space of the first cavity 1 is compressed, so that the menstrual blood in the tampon is desorbed. As a preferred embodiment, when the amount of menstrual blood is small, The buffer can be injected into the first chamber 1 after the tampon is placed.

The first chamber 1 further comprises a perforated partition 8 and a filtering device 7. In the present embodiment, the filtering device 7 is a filter. The perforated partition 8 is located at the lower end of the first chamber 1 and the filtering device 7 is located above the perforated partition 8. The filtering device 7 is used to filter tissue debris and other impurities in the menstrual blood. The blood and the like in the tampon are analyzed by the pressure difference mechanism 4, and the tissue fragments and impurities are filtered by the filtering device 7, and then passed through the perforated partition 8 to enter the second chamber 2. As a preferred embodiment, the second cavity 2 can also be pre-loaded with a buffer protection liquid to protect the test substance in the menstrual blood from rapid degradation or deterioration.

The second cavity 2 contains a second cavity wall 24 and a bottom plate 25. The second chamber 2 is located below the perforated partition 8.

The second cavity 2 is connected to the first cavity 1 by a thread or other fitting structure, and when the menstrual blood is collected, the entire menstrual blood collection system is in a completely sealed state. After the blood collection is completed, the second cavity 2 and the first cavity 1 are separated, and the second cavity 2 is sealed by a sealing cover or other sealing device, or directly connected to a third-party instrument, and further processed by a third-party related instrument and technical means. Analyze the physiological and biochemical indicators in women's menstrual blood to diagnose the occurrence of diseases and check the physiological status of women.

Embodiment 2 A blood transfusion detecting device

The menstrual blood detecting device shown in Fig. 2 comprises a cylindrical first cavity 1 and a second cavity 2 located below the first cavity 1, the second cavity 2 containing a detection system. The menstrual blood is desorbed and filtered in the first cavity, and enters the detection system of the second cavity to perform physiological and biochemical indicators.

The first chamber 1 further comprises a perforated partition 8 and a filtering device 7. In the present embodiment, the filtering device 7 is a filter. The perforated partition 8 is located at the lower end of the first chamber 1 and the filtering device 7 is located above the perforated partition 8. The filtering device 7 is used to filter tissue debris and other impurities in the menstrual blood. The blood and the like in the tampon are analyzed by the pressure difference mechanism 4, and the tissue fragments and impurities are filtered by the filtering device 7, and then passed through the perforated partition 8 to enter the second chamber 2.

The second cavity 2 contains a second cavity wall 24. The second chamber 2 is located below the perforated partition 8.

The second chamber 2 contains a detection system including a sensor, a wireless signal transmission device 14, and a power supply device 15.

In this embodiment, the sensor is a protein sensor. Further, the protein sensor comprises a protein chip 9, an excitation light source 10, a switch 11, a lens 12, a photoelectric converter, and a signal amplifier 13.

Further, the protein chip 9 is located below the first cavity 1. The protein chip 9 includes a substrate 19 and a plurality of sample cells 20 as shown in FIG. After the menstrual blood in the first cavity 1 is desorbed and filtered, the egg entering the detection system The white chip 9, in the sample cell 20 of the protein chip 9, the antigen to be tested and the like specifically binds to the fluorescently labeled probe in the sample cell, and emits fluorescence under the irradiation of the excitation light.

Further, the protein chip 9 is a transparent material, and the fluorescence of the protein chip 9 can pass through the bottom of the sample cell 20, is collected by the lens 12 and converted into an electrical signal by the photoelectric converter and the signal amplifier 13 and amplified, amplified. The electrical signal is transmitted to the electronic device 17 through the wireless signal transmission device 14. The electronic device 17 is equipped with corresponding software, and the processing and analysis of the collected data is analyzed by the software, thereby finally realizing online and real-time understanding of the user's health condition.

The excitation light source 10 is fixed on the inner wall of the second cavity wall 24 below the protein chip 9. On the outer wall of the second cavity wall 24, a switch 11 for controlling the excitation light source 10 is further disposed. In this embodiment, the excitation light source 10 is a violet LED.

The lens 12 is located below the protein chip 9 and concentrates the fluorescent signal emitted from the sample cell to the photoelectric converter and the signal amplifier 13. The photoelectric converter and signal amplifier 13 are connected to the wireless signal transmission device 14 by a circuit.

The bottom of the second cavity 2 is further provided with a power supply device 15 for supplying power to the excitation light source 10, the wireless signal transmission device 14, the photoelectric converter and the signal amplifier 13. Preferably, the bottom of the second cavity 2 is further provided with a battery replacement port 16.

In this embodiment, as a preferred embodiment, the blood collection system and the protein chip are all single-use components to prevent mutual contamination between different test samples. The other components of the inspection system are recyclable components.

In use, the tampon is placed in the first cavity, the blood is desorbed by the pressure difference mechanism, and the protein chip in the second cavity is passed through the filtering device. In the sample cell of the protein chip, the antigen to be tested and the like specifically binds to the fluorescently labeled probe, and emits fluorescence under the irradiation of the excitation light, and the emitted fluorescence is concentrated by the lens to the photoelectric converter to be converted into an electrical signal, and the electrical signal is further The signal amplifier is amplified, and the amplified electrical signal is transmitted to the electronic device through the wireless signal transmission device, and is analyzed and processed by the electronic device, thereby finally obtaining the health condition of the tester.

Embodiment 3 A blood transfusion detecting device

The menstrual blood detecting device shown in Fig. 4 comprises a cylindrical first cavity 1 and a second cavity 2 located below the first cavity 1, the second cavity containing a detection system. The menstrual blood is desorbed and filtered in the first cavity, and enters the detection system of the second cavity to perform physiological and biochemical indicators.

The first cavity 1 further comprises a perforated partition 8 and a filtering device 7, in the present embodiment, the filtering device 7 For the filter. The perforated partition 8 is located at the lower end of the first chamber 1 and the filtering device 7 is located above the perforated partition 8. The filtering device 7 is used to filter tissue debris and other impurities in the menstrual blood. The blood and the like in the tampon are analyzed by the pressure difference mechanism 4, and the tissue fragments and impurities are filtered by the filtering device 7, and then passed through the perforated partition 8 to enter the second chamber 2.

In this embodiment, the sensor is a protein sensor. Further, the protein sensor comprises a protein chip 9, an excitation light source 10, a switch 11, a dichroic filter 18, a lens 12, a photoelectric converter, and a signal amplifier 13.

Further, the protein chip 9 is located below the first cavity 1. The protein chip 9 includes a substrate 19 and a plurality of sample cells 20 as shown in FIG. After the menstrual blood in the first cavity 1 is desorbed and filtered, the protein chip 9 in the second cavity 2 is entered. In the sample cell 20 of the protein chip 9, the antigen to be tested is specific to the fluorescently labeled probe in the sample cell. Sexually combined, emitting fluorescence under the illumination of excitation light.

Further, the protein chip 9 is a transparent material, and the fluorescence of the protein chip 9 can pass through the bottom of the sample cell 20, is collected by the lens 12 and converted into an electrical signal by the photoelectric converter and the signal amplifier 13 and amplified, amplified. The electrical signal is transmitted to the electronic device 17 through the wireless signal transmission device 14. The electronic device 17 is equipped with corresponding software, and the processing and analysis of the collected data is analyzed by the software, thereby finally realizing the health status of the tester online and in real time.

The excitation light source 10 is fixed under the protein chip 9, and the second chamber wall 24 is provided on the inner wall of the chamber. On the outer wall of the detecting device, a switch 11 for controlling the excitation light source 10 is further disposed. In this embodiment, the excitation light source is a violet LED.

The dichroic filter 18 is located below the protein chip 9 at the same horizontal level as the excitation light source 10. The dichroic filter 18 reflects the excitation light to the protein chip, excites the specifically bound analyte to fluoresce, and the emitted fluorescence is transmitted through the dichroic filter 18.

The lens 12 is positioned below the dichroic filter 18 to concentrate the fluorescence transmitted through the dichroic filter 18 to the photoelectric converter and signal amplifier 13. The photoelectric converter and signal amplifier 13 are connected to the wireless signal transmission device 14 by a circuit.

The bottom of the second cavity 2 is further provided with a power supply device 15 for supplying power to the excitation light source 10, the wireless signal transmission device 14, the photoelectric converter and the signal amplifier 13. A battery exchange port 16 is also provided at the bottom of the second cavity 2.

When in use, the tampon is placed in the first cavity, and the blood is desorbed under the action of the pressure difference mechanism, and enters through the filtering device. Detection of protein chips in the system. In the sample cell of the protein chip, the antigen to be tested and the like specifically bind to the fluorescently labeled probe, and the excitation light is reflected into the sample cell through the dichroic filter, and the specific binding is performed under the illumination of the laser light. The measured substance emits fluorescence, and the emitted fluorescence passes through the dichroic filter. Under the action of the lens, it is concentrated to the photoelectric converter and converted into an electrical signal. The electrical signal is amplified by the signal amplifier, and the amplified electrical signal is transmitted through the wireless signal. The device is transmitted to the electronic device and analyzed and processed by the electronic device to finally obtain the health condition of the tester.

Embodiment 4 A blood transfusion detecting device

The menstrual blood detecting device shown in Fig. 5 comprises a cylindrical first cavity 1 and a second cavity 2 located below the first cavity 1, the second cavity containing a detection system. The menstrual blood is desorbed and filtered in the first cavity, and enters the detection system of the second cavity to perform physiological and biochemical indicators.

The protein chip 9 is located at the lower end of the second cavity 2. The protein chip 9 includes a substrate 19 and a plurality of sample cells 20 as shown in FIG. After the menstrual blood in the first cavity 1 is desorbed and filtered, the protein chip 9 of the detection system is entered. In the sample cell 20 of the protein chip 9, the antigen to be tested and the like are specifically combined with the fluorescently labeled probe in the sample pool. Fluorescence is emitted by the excitation light.

The excitation light source 10 is fixed above the protein chip 9, and on the inner wall of the second cavity wall 24, on the outer wall of the second cavity wall 24, a switch 11 for controlling the excitation light source 10 is further disposed.

Above the excitation light source 10, a first baffle 21 is further provided for protecting the excitation light source 10 from contamination by the menstrual blood sample, and at the same time, has a certain drainage effect on the menstrual blood.

The dichroic filter 18 is located above the protein chip 12 at the same horizontal level as the excitation light source 10. The dichroic filter 18 reflects the excitation light to the protein chip, excites the specifically bound analyte to fluoresce, and the emitted fluorescence is transmitted through the dichroic filter 18.

The lens 12 is positioned above the dichroic filter 18 to concentrate the fluorescence transmitted through the dichroic filter 18 to the photoelectric converter and signal amplifier 13. The photoelectric converter and signal amplifier 13 are connected to the wireless signal transmission device 14 by a circuit.

The photoelectric converter and signal amplifier 13 are located above the lens 12, and convert the fluorescent signal collected by the lens 12 into an electrical signal and amplify.

The wireless signal transmission device 14 is located below the apertured partition 8 and is connected to the photoelectric converter and the signal amplifier 13 through a circuit.

Surrounding the wireless signal transmission device 14, the photoelectric converter and the signal amplifier 13, there is a second baffle 22 for protecting the wireless signal transmission device 14, and the photoelectric converter and the signal amplifier 13 from contamination by the menstrual blood sample, and simultaneously draining The blood sample is applied to the protein chip 9.

The bottom of the second cavity 2 is further provided with a power supply device 15 which is located below the protein chip. The power supply device is used to supply power to the sensor, the excitation light source, and the wireless signal transmission device. A battery exchange port 16 is also provided at the bottom of the second cavity 2.

In use, the tampon is placed in the first cavity, and the blood is desorbed under the action of the pressure difference mechanism, and enters the detection system through the filtering device, and enters the protein chip through the blood under the drainage of the first baffle and the second baffle. Sample cell. In the sample cell, the antigen to be tested and the like specifically binds to the fluorescently labeled probe, and emits fluorescence under the irradiation of the excitation light, and the emitted fluorescence is concentrated by the lens to the photoelectric converter to be converted into an electrical signal, and the electrical signal is then passed through the signal amplifier. The amplified, amplified electrical signal is transmitted to the electronic device through the wireless signal transmission device, and is analyzed and processed by the electronic device to finally obtain the health condition of the tester.

Claims

A menstrual blood collection system characterized by comprising a cylindrical first cavity, a second cavity below the first cavity.
The menstrual blood collection system according to claim 1, wherein said first cavity is a tampon storage chamber;

Preferably, the first cavity is a space defined by a first cavity wall of a cylindrical shape, an elliptical cylinder shape, a prismatic shape or an irregular column shape, and a partition plate located below the first cavity body; The shape of the cavity wall is preferably cylindrical;

Preferably, the inner diameter of the chamber wall is not less than the outer diameter of the tampon; more preferably, the inner diameter of the chamber wall is not less than 2 cm;

More preferably, the inner diameter of the chamber wall is not less than 4 cm;

Preferably, a cover is arranged above the first cavity,

Preferably, the partition plate is a perforated partition; the lower portion of the partition is a second cavity defined by the partition and the second cavity wall below the partition; preferably, the second cavity wall is disposed below Have a bottom plate;

Optionally, the first cavity wall and the second cavity wall are hollow or non-hollowed;

Preferably, a filter device is disposed above the perforated partition; preferably, the filter device is a filter membrane, a filter, a filter, a filter gel or a filter column; more preferably a filter membrane.
The menstrual blood collection system according to claim 2, wherein said first cavity is provided with a pressure difference mechanism.

The pressure difference mechanism is a pressure applying device, further, a piston or a diaphragm, and the pressing device is located under the cover or the first cavity wall;

Preferably, the pressing device is a piston located under the cover;

Alternatively, the pressure difference mechanism is a vacuum device, and further, one of a vacuum pump, a piston that can be stretched, or a diaphragm.
A menstrual blood detecting device comprising the menstrual blood collection system according to any one of claims 1 to 3, wherein the second cavity is provided with a detection system.
The menstrual blood detecting apparatus according to claim 4, wherein said detecting system comprises one or more of a physical detecting means, a chemical detecting means, and a biological detecting means.
The menstrual blood detecting device according to claim 5, wherein said detecting system comprises a sensor;

Preferably, the sensor is a MEMS sensor;

Preferably, the sensor is one or more of a physical sensor, a chemical sensor and a biosensor;

The physical sensor is one or more of a humidity sensor, a temperature sensor, a thermal sensor, a weight sensor, and a volume sensor;

The chemical sensor is an ion sensitive sensor, a gas sensor, and a pH sensor;

The biosensor is one or more of a microbial sensor, an immunosensor, a tissue sensor, a cell sensor, an enzyme sensor, a nucleic acid sensor, and a protein sensor.
The menstrual blood detecting device according to claim 6, wherein said detecting system further comprises a wireless signal transmitting device and a power supply device;

Preferably, the wireless signal transmission device is a WiFi chip, a Bluetooth chip or an RFID chip;

Preferably, the power supply device is one or more of a lithium battery, a rechargeable battery, and a dry battery.
The menstrual blood detecting device according to claim 6, wherein said sensor is a protein sensor.
The blood transfusion detecting device according to claim 8, wherein said protein sensor comprises a protein chip, an excitation light source, a photoelectric converter, and a signal amplifier;

Preferably, the excitation light source comprises a switch and an automatic delay closing device.

Preferably, the protein chip comprises a substrate and a sample cell;

The substrate is a transparent material or an opaque material. When the excitation light source is located above the protein chip, the substrate is transparent or opaque; when the excitation light source is located above the protein chip, the substrate is transparent. Material.
The menstrual blood test system according to any one of claims 5 to 9 for detecting tissue cells/bacteria/fungus/virus/tumor markers/HPV antigens/enzymes/trace elements/pH/hormone/menstrual blood volume in female menstrual blood. Detection system.