WO2017121019A1 - Antimicrobial composition - Google Patents

Abstract

The present invention provides an antimicrobial composition. The composition comprises two active components, A and B. Active component A is a structural compound represented by formula (I), and active component B is an organic copper or inorganic copper antimicrobial agent. A weight ratio between the two components is 1:30 to 15:1. The present invention also provides a preparation method and use of the composition. An experimental result has indicated that the antimicrobial composition provided in the invention is significantly more effective. More importantly, application costs are lowered due to a reduced application amount. The antimicrobial composition can effectively prevent a specific fungal disease in a crop. By combining antimicrobial agents with different mechanisms and modes of action, the application amount of an individual antimicrobial agent can be effectively lowered. The invention is useful in expanding the antimicrobial spectrum, slowing fungal growth and development of fungal resistance, and increasing preventive effects.

Description

Fungicide composition Technical field

The present invention relates to the field of agricultural plant protection, and more particularly to a bactericidal composition having improved properties, and more particularly to a bactericidal composition comprising a benzisothiazolinone and a copper-containing formulation.

Background technique

Benzoisothiazolinones are a new type of broad-spectrum fungicide mainly used for the prevention and treatment of various bacterial and fungal diseases such as cereal crops, vegetables and fruits. The mechanism of bactericidal action mainly includes destroying the nuclear structure of the pathogen, causing it to lose the heart part and failing to death and interfering with the metabolism of the pathogenic cells, causing its physiological disorder and ultimately leading to death. It can effectively protect plants from pathogens in the early stage of disease occurrence. Increasing the dosage after disease occurrence can obviously control the spread of pathogens, thus achieving the dual functions of protection and eradication.

Copper preparation pesticides have a good preventive effect on common fungal and bacterial diseases. The copper preparations commonly used in agriculture are mainly divided into organic copper and inorganic copper. The inorganic copper mainly includes copper sulfate or basic copper sulfate, cuprous oxide and copper hydroxide. The common organic copper in agriculture is mainly copper complex, which is common. There are copper succinate, copper acetate, copper octoate, copper ruthenate, copper amide or copper rosinate.

Copper sulphate can be used to kill fungi. It is mixed with lime water to form Bordeaux mixture, which is used as a fungicide to control fungi on crops such as lemon and grape. Dilute solutions are used for sterilization in aquariums and for removing snails. Since copper ions are toxic to fish, the amount must be strictly controlled. Most fungi can be killed with very low concentrations of copper sulfate, and E. coli can also be controlled. In addition, the aquaculture industry is also used as the main raw material for trace element copper in feed additives.

Copper hydroxide is a blue powder and is a protective broad-spectrum fungicide. It is suitable for the main fungi and bacterial diseases of crops such as melon, fruit and vegetables. After preparation, the drug solution is stable and the diffusion performance is good. After spraying, it has strong adhesion and is resistant to rain. It can release copper ions stably and slowly. Generally, it is not easy to produce phytotoxicity to crops, and bacteria are not easy to produce drug resistance. At the same time, it can treat fungi and bacterial diseases, and it is safe for humans and animals.

The bactericide of multi-site action of basic copper sulphate has a fine particle size, good dispersibility, and is resistant to rain and wash, and can firmly adhere to the surface of the plant to form a protective film. The basic copper sulphate relies on water on the surface of the plant. Acidification, the gradual release of copper ions, inhibition of fungal spore germination and mycelial development, can effectively control the fungal and bacterial diseases of crops.

Cuprous oxide is a protective fungicide that effectively inhibits the growth of mycelium, destroys its reproductive organs, and prevents spread. Used for seed treatment and foliar spray. Seed dressing to control powdery mildew, leaf spot, blight, scab and rot, can be used for spinach Soaking seeds, beets, tomatoes, peppers, peas, pumpkins, kidney beans and melon seeds can also be sprayed to control fruit tree diseases. It can also be used for seed dressing to kill cockroaches and snails.

Acrylate copper sulphate is a mixture of succinic acid copper, glutaric acid copper and copper adipate copper, which is a protective fungicide. The exchange of copper ions with the cations on the surface of the pathogen membrane causes the protein on the cell membrane of the pathogen to coagulate, and at the same time, part of the copper ions penetrate into the cells of the pathogen to bind with certain enzymes, affecting its activity. It can be used to control bacterial bacterial leaf spot and has a stimulating effect on plant growth.

Copper acetate is formed by complexing acetic acid with copper. In the agricultural production activities, 20% copper acetate wettable powder is commonly used to control the diseases of various crops. The control targets include squatting, anthracnose, wilt, virus disease, etc. . Suitable crops include cucumber, watermelon, onion, tomato, pepper, eggplant and other vegetables and cotton, rice and other crops.

Copper octoate and copper ruthenate are bacterial and fungal diseases that mainly control crops. Such as citrus canker disease, cucumber bacterial leaf spot, rice bacterial leaf streak and so on.

Complex ammonia copper is a mixed agricultural fungicide with tetraammonium complex copper salt. It has strong systemic absorption and is mainly protective. It has a certain eradication effect. It is mainly used to control citrus canker disease, watermelon wilt, and rice stalk. Sick and so on. It has a certain promoting effect on the growth of crops such as cotton and watermelon. Mainly through the copper ions to play a bactericidal effect, the copper ions exchange with the cations such as K ⁺ ions and H ⁺ ions on the surface of the pathogenic bacteria cell membrane, so that the proteins on the cell membrane of the pathogenic bacteria are coagulated, and some copper ions penetrate into the cells of the pathogenic bacteria to bind with certain enzymes. Affecting its activity, copper ammonia has a certain promoting effect on the growth of cotton seedlings, watermelons, etc., and plays a certain role in disease resistance and yield increase. Ammonium copper can prevent and cure various diseases caused by fungi, bacteria and molds, and can promote deep roots and leaves, increase chlorophyll content, enhance photosynthesis and drought resistance, and have obvious effects of increasing yield.

Copper rosinate is a new type of copper bactericidal pesticide with high efficiency, low toxicity and broad spectrum. It has the characteristics of long-lasting effect and convenient use. It overcomes many shortcomings of the original Bordeaux mixture and is an ideal fungicide for replacing Bordeaux mixture. And there is a dual role of preventive protection and treatment. It can be used to control common plant diseases caused by various fungi and bacteria, and has obvious stimulating growth effect on vegetables. It can be alternated with other fungicides and has good spraying effect. It is used to control various vegetable diseases such as melon downy mildew, epidemic disease, black star disease, anthracnose, bacterial angular spot disease, eggplant blight, tomato late blight.

Practical pesticide experience has shown that repeated and specific application of an active compound to control harmful fungi will in many cases lead to rapid selectivity of fungal strains, and in order to reduce the risk of selectivity of resistant fungal strains, different activities are currently used. A mixture of compounds to control harmful fungi. By combining active compounds having different mechanisms of action, resistance can be delayed, application rates can be reduced, and cost of control can be reduced.

Summary of the invention

The purpose of the invention is to screen out different germicidal sources for the resistance of the bactericide in practical application and the problem of soil residue. The compound fungicide is compounded to obtain a new fungicide composition to improve the control effect of the fungicide, delay the generation of resistance, reduce the application amount, and reduce the cost of prevention.

Another object of the present invention is to provide a process for the preparation of a bactericidal composition comprising two active ingredients A and B and for the control of crop diseases in the agricultural sector.

The object of the invention can be achieved by the following measures:

A synergistic fungicidal composition comprising two active components A and B, wherein active component A is a structural compound having formula (I), and active component B is selected from inorganic copper containing Or one of organic copper bactericides.

In the formula (I), R is selected from H or a C ₁ - C ₈ alkyl group.

In a preferred embodiment, the organocopper bactericide in active component B is a copper-containing complex.

In a more preferred embodiment, the inorganic copper bactericide in the active component B is selected from the group consisting of copper sulfate, basic copper sulfate, cuprous oxide or copper hydroxide, and the organic copper bactericide is selected from the group consisting of succinic acid. One of copper, copper acetate, copper octoate, copper ruthenate, copper amide or copper rosinate.

The C ₁ -C ₈ alkyl group in the present invention means a linear or branched alkyl group having 1 to 8 carbon atoms, and includes a C ₁ alkyl group (e.g., methyl group) and a C ₂ alkyl group (e.g., ethyl group). , C ₃ alkyl (such as n-propyl, isopropyl), C ₄ alkyl (such as n-butyl, isobutyl, tert-butyl, sec-butyl), C ₅ alkyl (such as n-pentyl, etc.) , C ₆ alkyl, C ₇ alkyl, C ₈ alkyl. It includes, but is not limited to, C ₁ -C ₆ alkyl, C ₁ -C ₅ alkyl, C ₁ -C ₄ alkyl, and the like.

In a preferred embodiment, R is selected from H or a C ₁ -C ₄ alkyl group.

In a more preferred embodiment, R is selected from the group consisting of H, -CH ₃ or -C ₄ H ₉ .

In the formula (I), when R is H, A is 1,2-benzisothiazolin-3-one (abbreviated as BIT in the specification).

In the formula (I), when R is CH ₃ , A is 2-methyl-1,2-benzisothiazolin-3-one (abbreviated as MBIT in the specification).

In the formula (I), when R is C ₄ H ₉ , A is 2-butyl-1,2-benzisothiazolin-3-one, and the "butyl group" in the formula may be selected from n-butyl group. Isobutyl, tert-butyl, sec-butyl, collectively referred to as 2-butyl-1,2-benzisothiazolin-3-one (in the specification, n-butyl-1,2-benzisothiazoline) -3-ketone abbreviation BBIT).

The copper octoate in the present invention is a complex copper, which may be a linear copper ortho-octanoate or other form of a octyl group containing a branched alkyl group. Acid copper.

The copper ruthenate in the present invention is a complex copper, and may be a linear copper orthosilicate or other form of copper ruthenate containing a branched alkyl group.

The inventors have found through experiments that the composition of the present invention is effective for controlling bacterial or fungal diseases of crops, and more importantly, the application amount is reduced and the use cost is lowered. The compounds containing component A and component B have different structural types and different mechanisms of action. The combination of the two can expand the bactericidal spectrum, and can delay the generation and development of pathogen resistance to a certain extent, and component A and group There is no cross-resistance between points B.

The weight ratio between the two components in the bactericidal composition of the present invention is from 1:30 to 15:1, preferably from 1:25 to 10:1, further preferably from 1:20 to 10:1, more preferably 1:20. 1:1. In order to make the synergistic effect between the two components more significant, the weight ratio between the two components of the components A and B can be further optimized to 1:20 to 8:1, and a particularly preferred ratio is 1:20~5:1. In one version, the weight ratio between the two components can be 1:30, 1:29, 1:28, 1:27, 1:26, 1:25, 1:24, 1:24, 1: 22, 1:21, 1:20, 1:19, 1:18, 1:17, 1:16, 1:15, 1:14, 1:13, 1:12, 1:11, 1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5: 1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 2:3, 3:2 2:5, 5:2, 3:4, 4:3, 5:2, 2:5, 5:3, 3:5, 5:4, 4:5 are selected among these ratios. These ratios are understood to be weight ratios and may also include molar ratios.

The composition of the present invention can be made into a pesticide-acceptable dosage form from the active ingredient and agrochemical adjuvant or adjuvant. Further, the composition comprises from 5 to 80% by weight of the active ingredient and from 95 to 20% by weight of the pesticide adjuvant to form a pesticide-acceptable dosage form.

The present invention provides the use of a bactericidal composition comprising component A and component B for controlling crop diseases in the agricultural sector, in particular for controlling fungi or bacteria of certain crops.

The above composition may specifically comprise an agrochemical adjuvant or an auxiliary such as one or more of a carrier, a solvent, a dispersing agent, a wetting agent, an adhesive, a thickener, a binder, a surfactant or a fertilizer. Common auxiliaries can be mixed during the application.

Suitable auxiliaries or auxiliaries may be solid or liquid, they are usually materials commonly used in the processing of dosage forms, such as natural or regenerated minerals, solvents, dispersants, wetting agents, adhesives, thickeners, binders. .

In order to provide a use effect, the composition of the present invention can be processed into a preparation of various dosage forms with a fertilizer, or co-administered or mixed with a fertilizer. Suitable fertilizers include one or more of a large number of elements such as nitrogen, phosphorus, potassium, or the like, or one or more trace elements of copper, iron, manganese, zinc, boron, calcium, magnesium, sulfur, or the like, or A mixture of one or two of a fertilizer containing humic acid, amino acid, or the like.

The method of application of the compositions of the invention comprises the use of the compositions of the invention for aerial parts of plants, in particular leaves or foliage. You can choose to soak or apply to the surface of the control object. The frequency of administration and the amount administered will depend on the biological and climatic conditions of the pathogen. The plant growth site, such as rice fields, may be wetted with a liquid formulation of the composition, or the composition may be applied to the soil in solid form, such as in the form of granules (soil application), the composition may be passed from the soil to the plant through the roots of the plant. In vivo (systemic action).

The composition of the present invention can be prepared into various pesticide-acceptable dosage forms including, but not limited to, emulsifiable concentrates, suspending agents, wettable powders, water-dispersible granules, powders, granules, aqueous preparations, aqueous emulsions, microemulsions, poison baits. A mother liquor, a mother powder or the like. In a preferred embodiment, the dosage form of the invention employs a wettable powder, a suspending agent, a water-dispersible granule, an aqueous emulsion or a microemulsion. Depending on the nature of the compositions and the intended purpose and environmental conditions to which the compositions are to be applied, the compositions may be applied by spraying, misting, dusting, spreading or pouring, and the like.

The composition of the present invention can be prepared into various dosage forms by a known method, and the active ingredient and the auxiliary agent, such as a solvent, a solid carrier, and, if necessary, can be uniformly mixed and ground together with the surfactant to prepare a desired preparation. Dosage form.

The above solvent may be selected from aromatic hydrocarbons, preferably containing from 8 to 12 carbon atoms, such as a mixture of xylenes or substituted benzenes, phthalates such as dibutyl phthalate or dicaprylic acid, aliphatic hydrocarbons such as rings. Alkenes or paraffins, alcohols and glycols and their ethers and esters, such as ethanol, ethylene glycol, ethylene glycol monomethyl; ketones, such as cyclohexanone, highly polar solvents such as N-methyl-2 Pyrrolidone, dimethyl sulfoxide or dimethylformamide, and vegetable or vegetable oils such as soybean oil.

The above solid carriers, such as those used in powders and dispersibles, are typically natural mineral fillers such as talc, kaolin, montmorillonite or activated clay. In order to manage the physical properties of the composition, it is also possible to add a highly dispersible silicic acid or a highly dispersible adsorbent polymer carrier, such as a particulate adsorbent carrier or a non-adsorbing carrier, and a suitable particulate adsorbent carrier is porous, such as pumice, bentonite or Bentonite; a suitable non-adsorbing carrier such as calcite or sand. In addition, a large amount of pre-granulated materials of inorganic or organic nature can be used as a carrier, in particular dolomite.

Suitable surfactants according to the chemical nature of the active ingredient in the composition of the present invention are lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, alkaline earth metal or amine salts, alkylarylsulfonates, alkyl groups Sulfates, alkyl sulfonates, fatty alcohol sulphates, fatty acids and sulfated fatty alcohol glycol ethers, as well as condensates of sulfonated naphthalene and naphthalene derivatives with formaldehyde, naphthalene or naphthalene sulfonic acid with phenol and formaldehyde Condensate, polyoxyethylene octyl phenyl ether, ethoxylated isooctyl phenol, octyl phenol, nonyl phenol, alkyl aryl polyglycol ether, tributyl benzene polyglycol ether, three Stearyl phenyl polyglycol ether, alkyl aryl polyether alcohol, ethoxylated castor oil, polyoxyethylene alkyl ether, ethylene oxide condensate, ethoxylated polyoxypropylene, lauric acid poly Glycol ether acetal, sorbitol ester, lignin sulfite waste liquor and methyl cellulose.

When preparing a liquid dosage form, the active component A may be first dissolved in a basic substance to form a benzisothiazoline metal salt. Suitable basic materials include: alkali metal carbonates, alkali metal hydroxides (such as sodium hydroxide, potassium hydroxide), alkali metal alkoxy carbonates, alkali metal alkoxides or magnesium methoxide.

The two active ingredients in the compositions of the present invention exhibit synergistic effects, the activity of which is more pronounced than the expected sum of activity using a single compound, and the individual activity of a single compound. The synergistic effect is manifested by allowing for a reduced application rate, a broader fungicidal control profile, quicker effect, longer lasting control effect, better control of plant harmful fungi by only one or a few applications, and broadening of possible application. Intervals. These properties are particularly desirable in the practice of plant fungi control.

The bactericidal composition of the invention can be applied to the agricultural field for controlling crop diseases, and the specific diseases targeted include, but are not limited to, peach perforated bacterial perforation, tobacco wildfire, rice sheath blight, cucumber bacterial angular spot, cucumber Downy mildew, rice bacterial stripe disease, rice bacterial base rot, corn bacterial wilt, watermelon wilt, grape downy mildew, tomato bacterial wilt, eggplant bacterial wilt, rice blast, rice bacterium Stripe disease, pepper anthracnose, litchi ulcer disease, grape anthracnose, tobacco bacterial wilt, cucumber anthracnose, celery spot blotch, lotus root blight, strawberry powdery mildew, lettuce downy mildew, celery gray mold, Apricot bacterial perforation, peach tree canker, onion downy mildew, cotton bacterial leaf spot, cucumber bacterial leaf blight and so on.

The other characteristics exhibited by the bactericidal composition of the present invention are mainly as follows: 1. The compounding of the composition of the present invention has obvious synergistic effect; 2. Since the chemical composition of the two single agents of the present composition is greatly different, the effect The mechanism is completely different, there is no cross-resistance, and the problem of resistance caused by the separate use of the two single agents can be delayed; 3. The composition of the present invention is safe and safe for crops. It has been proved by experiments that the bactericidal composition of the invention has stable chemical properties, remarkable synergistic effect, and exhibits obvious synergistic effect and complementary effect on the control object.

detailed description

In order to make the objects, technical solutions and advantages of the present invention more comprehensible, the present invention will be further described in detail below with reference to the embodiments. It is understood that the specific embodiments described herein are intended to be illustrative of the invention and are not to be construed as limiting the scope of the invention. Within the scope of protection of the present invention.

The percentages in all formulations in the following examples are percentages by weight. The processing techniques of the various formulations of the compositions of the present invention are all prior art and may vary depending on the circumstances.

First, the dosage form preparation example

(1) Processing and examples of water-dispersible granules

The active ingredient active group A and the active component B, and the auxiliary agent and the filler are uniformly mixed according to the formula, and are pulverized into a wettable powder by a jet stream, and then added with a certain amount of water to be mixed and extruded, granulated, and dried and sieved. A water dispersible granule product.

1. Active component A (BIT) and active component B to prepare water-dispersible granules

Example 1: 31% BIT·copper sulfate water dispersible granules

BIT 1%, 30% copper sulfate, 5% potassium dodecylsulfonate, 3% ammonium sulfate, 4% potassium alkylnaphthalenesulfonate, and 100% light calcium carbonate.

Example 2: 16% BIT·copper sulfate water dispersible granules

BIT 15%, copper sulfate 1%, sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lauryl sulfate 3%, sodium lignin sulfonate 6%, diatomaceous earth to 100%.

Example 3: 31% BIT·basic copper sulfate water dispersible granules

BIT 1%, basic copper sulphate 30%, sodium carboxymethyl starch 2%, sodium dodecyl sulfonate 4%, xanthan gum 2%, sodium lignin sulfonate 6%, attapulgite to 100% .

Example 4: 16% BIT·basic copper sulfate water dispersible granules

BIT 15%, basic copper sulfate 1%, ammonium sulfate 1%, organic silicone 2%, sodium alginate 4%, sodium methylnaphthalene sulfonate formaldehyde condensate 2%, bentonite to 100%.

Example 5: 31% BIT· cuprous oxide water dispersible granules

BIT 1%, 30% cuprous oxide, 5% ammonium sulfate, 2% sodium alkylnaphthalene sulfonate, 3% sodium dodecyl sulfonate, and 100% light calcium carbonate.

Example 6: 16% BIT· cuprous oxide water dispersible granules

BIT 15%, cuprous oxide 1%, sodium methylnaphthalene sulfonate formaldehyde condensate 5%, sodium lignosulfonate 5%, sodium lauryl sulfate 3%, diatomaceous earth to 100%.

Example 7: 31% BIT·copper hydroxide water dispersible granules

BIT1%, copper hydroxide 30%, sodium lignosulfonate 4%, polyoxyalkylene aryl phenyl ether sulfate 2%, calcium carbonate 8%, white carbon black supplemented to 100%.

Example 8: 16% BIT·copper hydroxide water dispersible granules

BIT 15%, 1% copper hydroxide, 5% fatty alcohol polyoxyethylene ether, 4% calcium dodecylbenzenesulfonate, 3% magnesium carbonate, and 100% kaolin.

Example 9: 31% BIT·succinic acid copper copper dispersible granules

BIT1%, succinic acid copper 30%, sodium alkyl sulfosuccinate 8%, sodium carbonate 5%, starch supplement to 100%.

Example 10: 16% BIT·succinic acid copper copper dispersible granules

BIT 15%, succinic acid copper 1%, naphthalene sulfonate and alkyl substituted naphthalene sulfonate and formaldehyde condensate 8%, sodium bicarbonate 5%, sodium phosphate 6%, clay supplemented to 100%.

Example 11: 31% BIT·copper acetate water dispersible granules

BIT1%, 30% copper acetate, 9% sodium dodecyl naphthalene sulfonate, 6% potassium hydrogencarbonate, and sucrose supplemented to 100%.

Example 12: 16% BIT·copper acetate water dispersible granules

BIT 15%, 1% copper acetate, 3% sodium laurate, 5% potassium carbonate, and 100% kaolin.

Example 13: 31% BIT · copper octoate dispersible granules

BIT 1%, copper octoate 30%, and the remaining components were prepared in accordance with the procedure of Example 11.

Example 14: 16% BIT copper octoate dispersible granules

BIT 15%, copper octoate 1%, and the remaining components were prepared in accordance with the procedure of Example 12.

Example 15: 31% BIT·copper citrate water dispersible granules

BIT 1%, copper citrate 30%, and the remaining components were prepared in accordance with the procedure of Example 11.

Example 16: 16% BIT·copper citrate water dispersible granules

BIT 15%, copper citrate 1%, and the remaining components were prepared in accordance with the procedure of Example 12.

Example 17: 31% BIT·copper-copper water dispersible granules

BIT 1%, 30% copper sulphate, 2% sodium carboxymethyl starch, 4% sodium lignin sulfonate, 2% magnesium carbonate, and kaolin up to 100%.

Example 18: 16% MBIT·branched copper water dispersible granules

BIT 15%, 1% copper amide, 2% silicone, 3% methyl naphthalene sulfonate condensate, 4% sodium alginate, 4% sodium phosphate, 100% bentonite.

Example 19: 31% BIT·copper rosinate dispersible granules

BIT 1%, copper rosinate 30%, and the remaining components were prepared in accordance with the procedure of Example 17.

Example 20: 16% MBIT·copper rosinate dispersible granules

BIT 15%, 1% copper rosinate, and the remaining components were prepared in accordance with the procedure of Example 18.

2. Active component A (MBIT) and active component B to prepare water-dispersible granules

Example 21: 31% MBIT·copper sulfate water dispersible granules

MBIT 1%, copper sulfate 30%, and the remaining components were prepared in accordance with the method of Example 1.

Example 22: 16% MBIT·copper sulfate water dispersible granules

MBIT 15%, copper sulfate 1%, and the remaining components were prepared in the same manner as in Example 2.

Example 23: 31% MBIT·basic copper sulfate water dispersible granules

MBIT 1%, basic copper sulfate 30%, and the remaining components were prepared in the same manner as in Example 3.

Example 24: 16% MBIT·basic copper sulfate water dispersible granules

MBIT 15%, basic copper sulfate 1%, and the remaining components were prepared in the same manner as in Example 4.

Example 25: 31% MBIT· cuprous oxide water dispersible granules

MBIT 1%, cuprous oxide 30%, and the remaining components were prepared in accordance with the procedure of Example 5.

Example 26: 16% MBIT· cuprous oxide water dispersible granules

MBIT 15%, cuprous oxide 1%, and the remaining components were prepared in the same manner as in Example 6.

Example 27: 31% MBIT·copper hydroxide water dispersible granules

MBIT 1%, copper hydroxide 30%, and the remaining components were prepared in the same manner as in Example 7.

Example 28: 16% MBIT·copper hydroxide water dispersible granules

MBIT 15%, copper hydroxide 1%, and the remaining components were prepared in the same manner as in Example 8.

Example 29: 31% MBIT·succinic acid copper copper dispersible granules

MBIT 1%, succinic acid copper 30%, and the remaining components were prepared according to the method of Example 9.

Example 30: 16% MBIT·succinic acid copper copper dispersible granules

MBIT was 15%, succinic acid copper was 1%, and the remaining components were prepared in accordance with the method of Example 10.

Example 31: 31% MBIT·copper acetate water dispersible granules

MBIT 1%, copper acetate 30%, and the remaining components were prepared in accordance with the procedure of Example 11.

Example 32: 16% MBIT·copper acetate water dispersible granules

MBIT was 15%, copper acetate was 1%, and the remaining components were prepared in the same manner as in Example 12.

Example 33: 31% MBIT · copper octoate dispersible granules

MBIT 1%, copper octoate 30%, and the remaining components were prepared in accordance with the procedure of Example 11.

Example 34: 16% MBIT · copper octoate dispersible granules

MBIT 15%, copper octoate 1%, and the remaining components were prepared in accordance with the procedure of Example 12.

Example 35: 31% MBIT·copper citrate water dispersible granules

MBIT 1%, copper citrate 30%, and the remaining components were prepared in accordance with the procedure of Example 11.

Example 36: 16% MBIT·copper citrate water dispersible granules

MBIT 15%, copper citrate 1%, and the remaining components were prepared in accordance with the procedure of Example 12.

Example 37: 31% MBIT·copper copper water dispersible granules

MBIT 1%, copper ammonia 30%, and the remaining components were prepared in accordance with the procedure of Example 17.

Example 38: 16% MBIT·copper copper water dispersible granules

MBIT was 15%, copper ammonia was 1%, and the remaining components were prepared in the same manner as in Example 18.

Example 39: 31% MBIT·copper rosinate dispersible granules

MBIT 1%, copper rosinate 30%, and the remaining components were prepared in the same manner as in Example 19.

Example 40: 16% MBIT·copper rosinate dispersible granules

MBIT 15%, copper rosinate 1%, and the remaining components were prepared in the same manner as in Example 20.

3. Active component A (BBIT) and active component B to prepare water-dispersible granules

Example 41: 31% BBIT·copper sulfate water dispersible granules

BBIT 1%, copper sulfate 30%, and the remaining components were prepared in the same manner as in Example 1.

Example 42: 16% BBIT·copper sulfate water dispersible granules

BBIT 15%, copper sulfate 1%, and the remaining components were prepared in the same manner as in Example 2.

Example 43: 31% BBIT·basic copper sulfate water dispersible granules

BBIT 1%, basic copper sulfate 30%, and the remaining components were prepared in accordance with the procedure of Example 3.

Example 44: 16% BBIT·basic copper sulfate water dispersible granules

BBIT 15%, basic copper sulfate 1%, and the remaining components were prepared in the same manner as in Example 4.

Example 45: 31% BBIT· cuprous oxide water-dispersible granules

BBIT 1%, cuprous oxide 30%, and the remaining components were prepared in accordance with the procedure of Example 5.

Example 46: 16% BBIT· cuprous oxide water dispersible granules

BBIT 15%, cuprous oxide 1%, and the remaining components were prepared in accordance with the procedure of Example 6.

Example 47: 31% BBIT·copper hydroxide water dispersible granules

BBIT 1%, copper hydroxide 30%, and the remaining components were prepared in accordance with the procedure of Example 7.

Example 48: 16% BBIT·copper hydroxide water dispersible granules

BBIT 15%, copper hydroxide 1%, and the remaining components were prepared in accordance with the procedure of Example 8.

Example 49: 31% BBIT·succinic acid copper copper dispersible granules

BBIT 1%, succinic acid copper 30%, and the remaining components were prepared according to the method of Example 9.

Example 50: 16% BBIT·succinic acid copper copper dispersible granules

BBIT 15%, succinic acid copper 1%, the remaining components were prepared according to the method of Example 10.

Example 51: 31% BBIT·copper acetate water dispersible granules

BBIT 1%, copper acetate 30%, and the remaining components were prepared in accordance with the procedure of Example 11.

Example 52: 16% BBIT·copper acetate water dispersible granules

BBIT 15%, copper acetate 1%, and the remaining components were prepared in accordance with the procedure of Example 12.

Example 53: 31% BBIT·copper octoate water dispersible granules

BBIT 1%, copper octoate 30%, and the remaining components were prepared in accordance with the procedure of Example 11.

Example 54: 16% BBIT·copper octoate dispersible granules

BBIT 15%, copper octoate 1%, and the remaining components were prepared in accordance with the procedure of Example 12.

Example 55: 31% BBIT·copper citrate water dispersible granules

BBIT 1%, copper citrate 30%, and the remaining components were prepared in accordance with the procedure of Example 11.

Example 56: 16% BBIT·copper citrate water dispersible granules

BBIT 15%, copper citrate 1%, and the remaining components were prepared in accordance with the procedure of Example 12.

Example 57: 31% BBIT·copper copper water dispersible granules

BBIT 1%, copper ammonia 30%, and the remaining components were prepared in accordance with the procedure of Example 17.

Example 58: 16% BBIT·copper copper water dispersible granules

BBIT 15%, copper ammonia 1%, and the remaining components were prepared in accordance with the procedure of Example 18.

Example 59: 31% BBIT·copper rosinate dispersible granules

BBIT 1%, copper rosinate 30%, and the remaining components were prepared in accordance with the procedure of Example 19.

Example 60: 16% BBIT·copper rosinate dispersible granules

BBIT 15%, copper rosinate 1%, and the remaining components were prepared in the same manner as in Example 20.

(2) Processing and examples of suspending agents

The active ingredient active group A and the active component B, and the components such as a dispersing agent, a wetting agent, a thickener and water are uniformly mixed according to the formula, and after being sanded and/or sheared at a high speed, a semi-finished product is obtained. After the analysis, the water is mixed and evenly filtered to obtain the finished product.

1. Active component A (BIT) and active component B to prepare suspension agent

Example 61: 16% BIT·copper sulfate suspension

BIT 1%, copper sulfate 15%, xanthan gum 3%, bentonite 4%, aluminum magnesium silicate 2%, ethylene glycol 2%, lignosulfonic acid Sodium 7%, water to 100%.

Example 62: 20% BIT·copper sulfate suspension

BIT 15%, copper sulfate 5%, bentonite 4%, glycerol 3%, sodium methylnaphthalene sulfonate formaldehyde condensate 5%, water to 100%.

Example 63: 16% BIT·basic copper sulfate suspension

BIT 1%, basic copper sulfate 15%, white carbon black 3%, glycerol 6%, sodium benzoate 2%, fatty alcohol polyoxyethylene ether phosphate 7%, water to 100%.

Example 64: 20% BIT·basic copper sulfate suspension

BIT 15%, basic copper 5%, white carbon 4%, ethylene glycol 5%, sodium lignin sulfonate 7%, xanthan gum 2%, water to 100%.

Example 65: 16% BIT· cuprous oxide suspension

BIT1%, cuprous oxide 15%, alkylphenol formaldehyde resin polyoxyethylene ether 2%, sodium lignosulfonate 4%, ethylene glycol 3%, polydimethylsiloxane 0.4%, xanthan gum 1% Sodium benzoate 0.25%, water to 100%.

Example 66: 20% BIT· cuprous oxide suspension

BIT15%, 5% cuprous oxide, special polyether modified polyorganosiloxane 4%, propylene glycol 3%, isooctanol 1%, alkylphenol based polyoxyethylene phosphate 3%, gum arabic 0.5%, The water is made up to 100%.

Example 67: 16% BIT·copper hydroxide suspension

BIT1%, copper hydroxide 15%, 5.2g, polynaphthaldehyde sulfonate sodium salt 4%, fatty alcohol polyoxyethylene ether 2%, glycerol 4%, n-octanol 1%, sodium alginate 0.5%, lactic acid 0.5%, water is made up to 100%.

Example 68: 20% BIT·copper hydroxide suspension

BIT15%, 5% copper hydroxide, 2% powder, 3% phenolic polyoxyethylene phosphate, 1% isooctyl alcohol, 1% gum arabic, 4% propylene glycol, 0.5% sodium benzoate, water-filled To 100%.

Example 69: 16% BIT·succinic acid copper suspending agent

BIT1%, acryl acid copper 15%, alkylphenol formaldehyde resin polyoxyethylene ether 3%, calcium lignosulfonate 5%, ethylene glycol 4%, polydimethylsiloxane 0.4%, xanthan gum 0.2%, sodium benzoate 0.2%, water to 100%.

Example 70: 20% BIT·succinic acid copper suspending agent

BIT 15%, 5% succinic acid copper, 2% fatty alcohol ethoxylate, 5% sodium polynaphthalene sulfonate, 4% ethylene glycol, 1% isooctyl alcohol, 0.9% sodium alginate, 0.5 lactic acid %, the water is made up to 100%.

Example 71: 16% BIT·copper acetate suspension

BIT1%, copper acetate 15%, alkylphenol-based polyoxyethylene ether 2%, ethylene glycol 4%, sodium benzoate 0.5% alkylnaphthalenesulfonic acid condensate 3%, polydimethylsiloxane 0.5%, Xanthan gum 0.3%, water to 100%.

Example 72: 20% BIT·copper acetate suspension

BIT15%, 5% copper acetate, 2% powder, 6% sodium naphthalene sulfonate, 4% urea, 0.5% polydimethylsiloxane, 0.5% xanthan gum, 0.8% sodium benzoate, water Make up to 100%.

Example 73: 16% BIT · copper octoate suspension

BIT 1%, copper octoate 15%, and the remaining components were prepared in the same manner as in Example 71.

Example 74: 20% BIT · copper octoate suspension

BIT 15%, copper octoate 5%, and the remaining components were prepared in accordance with the procedure of Example 72.

Example 75: 16% BIT·copper citrate suspension

BIT 1%, copper citrate 15%, and the remaining components were prepared in accordance with the procedure of Example 71.

Example 76: 20% BIT·copper citrate suspension

BIT 15%, 5% copper berylate, and the remaining components were prepared in accordance with the procedure of Example 72.

Example 77: 16% BIT·copper copper suspension

BIT1%, 15% ammonia copper, 1% gum arabic, 2% phenethyl phenol polyoxyethylene ether, 5% modified calcium lignosulfonate, 4% propylene glycol, 4% isooctyl alcohol, 0.1% sodium benzoate.

Example 78: 20% BIT·copper copper suspension

BIT 15%, 5% copper ammonia, 4% calcium lignosulfonate, 4% ethylene glycol, 2% phenethyl phenol polyoxyethylene ether, 0.5% polydimethylsiloxane, 0.2% gum arabic.

Example 79: 16% BIT · copper rosinate suspension

BIT1%, 15% copper rosinate, 3% fatty alcohol polyoxyethylene ether, 3% phenethyl phenol polyoxyethylene ether, 3% ethylene glycol, 2% isooctyl alcohol, 1% sodium alginate, gum arabic 0.2 %.

Example 80: 20% BIT · copper rosinate suspension

BIT 15%, 5% copper rosinate, 4% calcium lignosulfonate, 4% isopropyl glycol, 4% sodium naphthalene formaldehyde sulfonate, 4% iso-octanol, 0.8% sodium benzoate.

2. Preparation of suspending agent from active ingredient A (MBIT) and active ingredient B

Example 81: 16% MBIT·copper sulfate suspension

MBIT 1%, copper sulfate 15%, and the remaining components were prepared in the same manner as in Example 61.

Example 82: 20% MBIT·copper sulfate suspension

MBIT 15%, copper sulfate 5%, and the remaining components were prepared in accordance with the procedure of Example 62.

Example 83: 16% MBIT·basic copper sulfate suspension

MBIT 1%, basic copper sulfate 15%, and the remaining components were prepared in the same manner as in Example 63.

Example 84: 20% MBIT·basic copper sulfate suspension

MBIT 15%, basic copper sulfate 5%, and the remaining components were prepared in the same manner as in Example 64.

Example 85: 16% MBIT· cuprous oxide suspension

MBIT 1%, cuprous oxide 15%, and the remaining components were prepared in the same manner as in Example 65.

Example 86: 20% MBIT· cuprous oxide suspension

MBIT was 15%, 5% cuprous oxide, and the remaining components were prepared in accordance with the procedure of Example 66.

Example 87: 16% MBIT·copper hydroxide suspension

MBIT 1%, copper hydroxide 15%, and the remaining components were prepared in the same manner as in Example 67.

Example 88: 20% MBIT·copper hydroxide suspension

MBIT 15%, copper hydroxide 5%, and the remaining components were prepared in the same manner as in Example 68.

Example 89: 16% MBIT·succinic acid copper suspending agent

MBIT 1%, succinic acid copper 15%, and the remaining components were prepared in accordance with the procedure of Example 69.

Example 90: 20% MBIT·succinic acid copper suspending agent

MBIT was 15%, succinic acid copper 5%, and the remaining components were prepared in accordance with the procedure of Example 70.

Example 91: 16% MBIT·copper acetate suspension

MBIT 1%, copper acetate 15%, and the remaining components were prepared in the same manner as in Example 71.

Example 92: 20% MBIT·copper acetate suspension

MBIT 15%, copper acetate 5%, and the remaining components were prepared in accordance with the procedure of Example 72.

Example 93: 16% MBIT · copper octoate suspension

MBIT 1%, copper octoate 15%, and the remaining components were prepared in the same manner as in Example 71.

Example 94: 20% MBIT · copper octoate suspension

MBIT 15%, copper octoate 5%, and the remaining components were prepared in the same manner as in Example 72.

Example 95: 16% MBIT· copper citrate suspension

MBIT 1%, copper citrate 15%, and the remaining components were prepared in the same manner as in Example 71.

Example 96: 20% MBIT· copper citrate suspension

MBIT 15%, 5% copper ruthenate, and the remaining components were prepared in accordance with the procedure of Example 72.

Example 97: 16% MBIT·copper copper suspension

MBIT 1%, copper ammonia 15%, and the remaining components were prepared in accordance with the procedure of Example 77.

Example 98: 20% MBIT·copper copper suspension

MBIT was 15%, copper copper was 5%, and the remaining components were prepared in accordance with the procedure of Example 78.

Example 99: 16% MBIT · copper rosinate suspension

MBIT 1%, copper rosinate 15%, and the remaining components were prepared in the same manner as in Example 79.

Example 100: 20% MBIT · copper rosinate suspension

MBIT 15%, 5% copper rosinate, and the remaining components were prepared in accordance with the procedure of Example 80.

3. Preparation of suspending agent from active ingredient A (BBIT) and active ingredient B

Example 101: 16% BBIT·copper sulfate suspension

BBIT 1%, copper sulfate 15%, and the remaining components were prepared in accordance with the procedure of Example 61.

Example 102: 20% BBIT·copper sulfate suspension

BBIT 15%, copper sulfate 5%, and the remaining components were prepared in accordance with the procedure of Example 62.

Example 103: 16% BBIT·basic copper sulfate suspension

BBIT 1%, basic copper sulfate 15%, and the remaining components were prepared in the same manner as in Example 63.

Example 104: 20% BBIT·basic copper sulfate suspension

BBIT 15%, basic copper sulfate 5%, and the remaining components were prepared in accordance with the procedure of Example 64.

Example 105: 16% BBIT· cuprous oxide suspension

BBIT 1%, cuprous oxide 15%, and the remaining components were prepared in accordance with the procedure of Example 65.

Example 106: 20% BBIT· cuprous oxide suspension

BBIT 15%, 5% cuprous oxide, and the remaining components were prepared in accordance with the procedure of Example 66.

Example 107: 16% BBIT·copper hydroxide suspension

BBIT 1%, copper hydroxide 15%, and the remaining components were prepared in the same manner as in Example 67.

Example 108: 20% BBIT·copper hydroxide suspension

BBIT 15%, copper hydroxide 5%, and the remaining components were prepared in accordance with the procedure of Example 68.

Example 109: 16% BBIT·succinic acid copper suspending agent

BBIT 1%, succinic acid copper 15%, and the remaining components were prepared in accordance with the procedure of Example 69.

Example 110: 20% BBIT·succinic acid copper suspending agent

BBIT 15%, succinic acid copper 5%, and the remaining components were prepared according to the method of Example 70.

Example 111: 16% BBIT·copper acetate suspension

BBIT 1%, copper acetate 15%, and the remaining components were prepared in the same manner as in Example 71.

Example 112: 20% BBIT·copper acetate suspension

BBIT 15%, copper acetate 5%, and the remaining components were prepared in accordance with the procedure of Example 72.

Example 113: 16% BBIT·copper octoate suspension

BBIT 1%, copper octoate 15%, and the remaining components were prepared in the same manner as in Example 71.

Example 114: 20% BBIT · copper octoate suspension

BBIT 15%, copper octoate 5%, and the remaining components were prepared in accordance with the procedure of Example 72.

Example 115: 16% BBIT· copper citrate suspension

BBIT 1%, copper ruthenate 15%, and the remaining components were prepared in the same manner as in Example 71.

Example 116: 20% BBIT· copper citrate suspension

BBIT 15%, 5% copper ruthenate, and the remaining components were prepared in accordance with the procedure of Example 72.

Example 117: 16% BBIT·copper copper suspension

BBIT 1%, copper ammonia 15%, and the remaining components were prepared in accordance with the procedure of Example 77.

Example 118: 20% BBIT·copper copper suspension

BBIT 15%, 5% ammonia copper, and the remaining components were prepared in accordance with the procedure of Example 78.

Example 119: 16% BBIT · copper rosinate suspension

BBIT 1%, copper rosinate 15%, and the remaining components were prepared in accordance with the procedure of Example 79.

Example 120: 20% BBIT · copper rosinate suspension

BBIT 15%, 5% copper rosinate, and the remaining components were prepared in accordance with the procedure of Example 80.

(3) Processing and examples of wettable powders

The active ingredient A and the B active ingredient are sufficiently mixed with various auxiliary agents and fillers, and are pulverized by an ultrafine pulverizer to obtain a wettable powder.

1. Active component A (BIT) and active component B to prepare wettable powder

Example 121: 20% BIT·copper sulfate wettable powder

BIT 5%, copper sulfate 15%, sodium dodecyl benzene sulfonate 2%, calcium lignosulfonate 3%, bentonite 3%, attapulgite to 100%.

Example 122: 20% BIT·basic copper sulfate wettable powder

BIT 5%, basic copper sulphate 15%, pull-open powder 2%, bentonite 1.5%, alkyl polyoxyethyl ether sulfonate 1%, white Carbon black 2%, diatomaceous earth to make up to 100%.

Example 123: 20% BIT· cuprous oxide wettable powder

BIT 5%, cuprous oxide 15%, sodium lignin sulfonate 6%, alkyl sulfonate 7%, white carbon black 10%, kaolin to 100%.

Example 124: 20% BIT·copper hydroxide wettable powder

BIT 5%, copper hydroxide 15%, sodium lignin sulfonate 5%, white carbon black 3%, polyoxyethylene octyl phenyl ether 4%, diatomaceous earth to 100%.

Example 125: 20% BIT·succinic acid copper WP

BIT 5%, succinic acid copper 15%, polyoxyethylene octyl phenyl ether 2%, sodium lignosulfonate 1%, white carbon black 3%, diatomaceous earth to 100%.

Example 126: 20% BIT·copper acetate wettable powder

BIT 5%, copper acetate 15%, white carbon black 3%, sodium dodecylbenzene sulfonate 4%, sodium lignin sulfonate 5%, attapulgite to 100%.

Example 127: 20% BIT·copper octoate wettable powder

BIT 5%, 15% copper octoate, 3% sodium dodecyl benzene sulfonate, 4% open powder, 6% bentonite, and 100% attapulgite.

Example 128: 20% BIT·copper citrate wettable powder

BIT 5%, copper citrate 15%, calcium lignosulfonate 4%, alkyl polyoxyethyl ether sulfonate 3%, bentonite 2%, white carbon black 4%, diatomaceous earth to 100%.

Example 129: 20% BIT·lumenium copper wettable powder

BIT 5%, 15% lycopene copper, 4% polyoxyethylene octyl phenyl ether, 5% sodium lignosulfonate, 6% white carbon black, and diatomaceous earth up to 100%.

Example 130: 20% BIT··copper rosinate wettable powder

BIT 5%, 15% copper rosinate, 2% alkyl polyoxyethyl ether sulfonate, 6% open powder, 5% bentonite, 4% white carbon black, and diatomaceous earth up to 100%.

2. Active component A (MBIT) and active component B to prepare wettable powder

Example 131: 20% MBIT·copper sulfate wettable powder

MBIT 5%, copper sulfate 15%, and the remaining components were prepared in accordance with the procedure of Example 121.

Example 132: 20% MBIT·basic copper sulfate wettable powder

MBIT 5%, basic copper sulphate 15%, and the remaining components were prepared according to the procedure of Example 122.

Example 133: 20% MBIT· cuprous oxide wettable powder

MBIT 5%, cuprous oxide 15%, and the remaining components were prepared in accordance with the procedure of Example 123.

Example 134: 20% MBIT·copper hydroxide wettable powder

MBIT 5%, copper hydroxide 15%, and the remaining components were prepared in accordance with the procedure of Example 124.

Example 135: 20% MBIT·succinic acid copper WP

MBIT 5%, succinic acid copper 15%, and the remaining components were prepared in accordance with the procedure of Example 125.

Example 136: 20% MBIT·copper acetate wettable powder

The MBIT was 5%, copper acetate was 15%, and the remaining components were prepared in the same manner as in Example 126.

Example 137: 20% MBIT·copper octoate wettable powder

MBIT 5%, copper octoate 15%, and the remaining components were prepared according to the procedure of Example 127.

Example 138: 20% MBIT·copper citrate wettable powder

MBIT 5%, copper citrate 15%, and the remaining components were prepared according to the procedure of Example 128.

Example 139: 20% MBIT·lumena copper wettable powder

MBIT 5%, copper ammonia 15%, and the remaining components were prepared according to the procedure of Example 129.

Example 140: 20% MBIT··copper rosinate wettable powder

MBIT 5%, copper rosinate 15%, and the remaining components were prepared in accordance with the procedure of Example 130.

3. Active component A (BBIT) and active component B to prepare wettable powder

Example 141: 20% BBIT·copper sulfate wettable powder

BBIT 5%, copper sulfate 15%, and the remaining components were prepared in accordance with the procedure of Example 121.

Example 142: 20% BBIT·basic copper sulfate wettable powder

BBIT 5%, basic copper sulphate 15%, and the remaining components were prepared according to the procedure of Example 122.

Example 143: 20% BBIT· cuprous oxide wettable powder

BBIT 5%, cuprous oxide 15%, and the remaining components were prepared in accordance with the procedure of Example 123.

Example 144: 20% BBIT·copper hydroxide wettable powder

BBIT 5%, copper hydroxide 15%, and the remaining components were prepared in accordance with the procedure of Example 124.

Example 135: 20% BBIT·succinic acid copper WP

BBIT 5%, succinic acid copper 15%, and the remaining components were prepared in accordance with the procedure of Example 125.

Example 146: 20% BBIT·copper acetate wettable powder

BBIT 5%, copper acetate 15%, and the remaining components were prepared according to the procedure of Example 126.

Example 147: 20% BBIT·copper octoate wettable powder

BBIT 5%, copper octoate 15%, and the remaining components were prepared according to the procedure of Example 127.

Example 148: 20% BBIT·copper citrate wettable powder

BBIT 5%, copper citrate 15%, and the remaining components were prepared according to the procedure of Example 128.

Example 149: 20% BBIT·lumenium copper wettable powder

BBIT 5%, copper ammonia 15%, and the remaining components were prepared in accordance with the procedure of Example 129.

Example 150: 20% BBIT··copper rosinate wettable powder

BBIT 5%, copper rosinate 15%, and the remaining components were prepared in accordance with the procedure of Example 130.

Second, the efficacy test

(1) Bioassay examples

According to the test grading standards, the incidence of the whole plant leaves was investigated, and the disease index and control effect were calculated.

The control effect is converted into the probability value (y), the liquid height (μg/ml) is converted into a logarithmic value (x), the virulence equation is calculated by the least squares method, and the neutral concentration EC50 is suppressed, and the virulence of the drug is calculated according to the method of Sun Yunpei. Exponential Co-toxicity Factor (CTC).

Measured virulence index (ATI) = (standard drug EC50 / test drug EC50) * 100

Theoretical virulence index (TTI) = A virulence index * Percentage of A in the mixture + B virulence index * Percentage of B in the mixture

Co-toxicity coefficient (CTC) = [mixture measured virulence index (ATI) / mixed theory virulence index (TTI)] * 100

CTC ≤ 80, the composition showed antagonism, 80 < CTC < 120, the composition showed an additive effect, CTC ≥ 120, and the composition showed synergistic effect.

1. BIT and organic copper or inorganic copper indoor activity test

(1) BIT and copper sulfate complex pairing toxicity test

Table 1. Analysis of the results of the determination of bacterial perforation virus in peach tree by BIT and copper sulfate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper sulfate	35.92	100	/	/
BIT	19.58	183.45	/	/
Copper sulfate: BIT=30:1	27.98	128.38	102.69	125.01
Copper sulfate: BIT=20:1	25.56	140.53	103.97	135.17
Copper sulfate: BIT=10:1	19.96	179.96	107.59	167.26
Copper sulfate: BIT = 1:1	18.86	190.46	141.73	134.38
Copper sulfate: BIT=1:10	15.84	226.77	175.86	128.95
Copper sulfate: BIT=1:15	16.09	223.24	178.23	125.26

The results (Table 1) showed that the control effect of BIT and copper sulphate on the bacterial perforation of peach trees was significantly improved, indicating that the two had a significant synergistic effect on the prevention and control of bacterial perforation of peach trees.

(2) BIT and basic copper sulphate complex test for tobacco wildfire virus

Table 2 Analysis of the results of the determination of BIT and basic copper sulphate for the determination of tobacco wildfire virus

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Basic copper sulphate	67.87	100	/	/
BIT	41.15	164.93	/	/
Basic copper sulfate: BIT=30:1	52.26	129.87	102.09	127.21
Basic copper sulfate: BIT=20:1	48.02	141.34	103.09	137.10
Basic copper sulfate: BIT=10:1	41.28	164.41	105.9	155.25
Basic copper sulphate: BIT = 1:1	37.57	180.65	132.47	136.37
Basic copper sulfate: BIT=1:10	32.83	206.73	159.03	130.00
Basic copper sulfate: BIT=1:15	33.27	204.00	160.87	126.81

The results (Table 2) showed that the control effect of BIT and basic copper sulphate on tobacco wildfire virus was significantly improved, indicating that the two had a significant synergistic effect on tobacco wildfire disease.

(3) BIT and cuprous oxide complex test for rice strain

Table 3. Analysis of the results of the determination of BIT and cuprous oxide in rice

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Cuprous oxide	28.92	100	/	/
BIT	18.13	159.51	/	/
Cuprous oxide: BIT=30:1	23.04	125.52	101.92	123.16
Cuprous oxide: BIT=20:1	20.77	139.24	102.83	135.41
Cuprous oxide: BIT=10:1	18.27	158.29	105.41	150.17
Cuprous oxide: BIT=1:1	15.76	183.50	129.76	141.42
Cuprous oxide: BIT=1:10	14.74	196.20	154.1	127.32
Cuprous oxide: BIT=1:15	14.94	193.57	155.79	124.25

The results (Table 3) showed that the control effect of BIT and cuprous oxide on rice scurocavirus was significantly improved, indicating that the two had a significant synergistic effect on rice sheath blight.

(4) BIT and copper hydroxide complex pairing cucumber bacterial keratosis test

Table 4. Analysis of the results of determination of bacterial keratosis virus in cucumber and copper hydroxide

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper hydroxide	58.87	100	/	/
BIT	36.26	162.36	/	/
Copper hydroxide: BIT=30:1	47.18	124.78	102.01	122.32
Copper hydroxide: BIT=20:1	43.39	135.68	102.97	131.76
Copper hydroxide: BIT=10:1	38.25	153.91	105.67	145.65

Copper hydroxide: BIT=1:1	32.83	179.32	131.18	136.70
Copper hydroxide: BIT=1:10	29.28	201.06	156.69	128.32
Copper hydroxide: BIT=1:15	30.07	195.78	158.46	123.55

The results (Table 4) showed that the control effect of BIT and copper hydroxide on cucumber bacterial keratosis was significantly improved, indicating that the two had a significant synergistic effect on cucumber bacterial keratosis.

(5) Determination of virulence of cucumber downy mildew by BIT and succinated copper

Table 5. Analysis of the results of the determination of BIT and succinated copper

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Amber	38.16	100	/	/
BIT	21.35	178.74	/	/
Aromatic acid copper: BIT=30:1	29.47	129.49	102.54	126.28
Aromatic acid copper: BIT=20:1	27	141.33	103.75	136.22
Acrylate copper: BIT=10:1	23.6	161.69	107.16	150.89
Acrylate copper acid: BIT = 1:1	19.36	197.11	139.37	141.43
Amber gum copper: BIT=1:10	16.77	227.55	171.58	132.62
Aromatic acid copper: BIT=1:15	17.21	221.73	173.82	127.56

The results (Table 5) showed that the control effect of BIT and succinated copper on the downy mildew of cucumber was significantly improved, indicating that the two had a significant synergistic effect on cucumber downy mildew.

(6) BIT and copper acetate complex determination of bacterial leaf streak virus in rice

Table 6. Analysis of the results of determination of bacterial bacterial streak virus in BIT and copper acetate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper acetate	46.81	100	/	/
BIT	28.32	165.29	/	/
Copper acetate: BIT=30:1	37.37	125.26	102.11	122.67
Copper acetate: BIT=20:1	35.29	132.64	103.11	128.64
Copper acetate: BIT=10:1	32.17	145.51	105.94	137.35
Copper acetate: BIT=1:1	26.96	173.63	132.65	130.89
Copper acetate: BIT=1:10	23.13	202.38	159.35	127.00
Copper acetate: BIT=1:15	23.81	196.60	161.21	121.95

The results (Table 6) showed that the control effect of BIT and copper acetate on rice bacterial leaf streak was significantly improved, indicating that the two had a significant synergistic effect on rice bacterial leaf streak.

(7) BIT and copper octoate complex determination of bacterial bacterial base rot

Table 7. Analysis of the results of determination of bacterial bacterial base rot virus in BIT and copper octoate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper octoate	41.52	100	/	/
BIT	26.95	154.06	/	/

Copper octoate: BIT=30:1	32.56	127.52	101.74	125.34
Copper octoate: BIT=20:1	30.7	135.24	102.57	131.86
Copper octoate: BIT=10:1	27.53	150.82	104.91	143.76
Copper octoate: BIT=1:1	24.66	168.37	127.03	132.54
Copper octoate: BIT=1:10	21.38	194.20	149.15	130.20
Copper octoate: BIT=1:15	21.66	191.69	150.68	127.22

The results (Table 7) showed that the control effect of BIT and copper octoate on rice bacterial base rot was significantly improved, indicating that the two had a significant synergistic effect on rice bacterial base rot.

(8) Determination of bacterial bacterial wilt virus in corn by complexation of BIT and copper citrate

Table 8. Analysis of the results of determination of bacterial wilt virus in corn by BIT and copper citrate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper citrate	36.09	100	/	/
BIT	25.12	143.67	/	/
Copper citrate: BIT=30:1	27.73	130.15	101.41	128.34
Copper citrate: BIT=20:1	26.33	137.07	102.08	134.28
Copper citrate: BIT=10:1	23.54	153.31	103.97	147.46
Copper citrate: BIT=1:1	22.24	162.28	121.84	133.19
Copper citrate: BIT=1:10	19.69	183.29	139.7	131.20
Copper citrate: BIT=1:15	20.2	178.66	140.94	126.77

The results (Table 8) showed that the control effect of BIT and copper citrate on corn bacterial wilt was significantly improved, indicating that the two had a significant synergistic effect on corn bacterial wilt.

(9) BIT and lycopene complex pairing for determination of watermelon wilt virus

Table 9. Analysis of the results of BIT and copper copper complex pairing with watermelon wilt

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper ammonia	31.24	100	/	/
BIT	23.52	132.82	/	/
Copper ammonia: BIT=30:1	25.09	124.51	101.06	123.21
Copper ammonia: BIT=20:1	23.42	133.39	101.56	131.34
Copper ammonia: BIT=10:1	21.43	145.78	102.98	141.56
Copper ammonia: BIT = 1:1	20.83	149.98	116.41	128.83
Copper ammonia: BIT=1:10	19.14	163.22	129.84	125.71
Copper ammonia: BIT=1:15	19.45	160.62	130.77	122.82

The results (Table 9) showed that the control effect of BIT and lycopene copper on watermelon wilt was significantly improved, indicating that the two had a significant synergistic effect on watermelon wilt.

(10) BIT and copper rosinate complex determination of grape downy mildew virus

Table 10. Analysis of the results of BIT and copper rosinate complexes

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper rosinate	46.82	100	/	/
BIT	29.35	159.52	/	/
Copper rosinate: BIT=30:1	35.97	130.16	101.92	127.71
Copper rosinate: BIT=20:1	33.3	140.60	102.83	136.73
Copper rosinate: BIT=10:1	30.08	155.65	105.41	147.66
Copper rosinate: BIT = 1:1	26.36	177.62	129.76	136.88
Copper rosinate: BIT=1:10	23.16	202.16	154.11	131.18
Copper rosinate: BIT=1:15	24.01	195.00	155.8	125.16

The results (Table 10) showed that the control effect of BIT and copper rosinate on grape downy mildew was significantly improved, indicating that the two had a significant synergistic effect on grape downy mildew.

2, MBIT and organic copper or inorganic copper compound toxicity test

(11) MBIT and copper sulfate complex pairing

Table 11. Analysis of the results of the determination of tomato green wilt virus by MBIT and copper sulfate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper sulfate	35.12	100	/	/
MBIT	21.09	166.52	/	/
Copper sulfate: MBIT=30:1	27.64	127.06	102.15	124.39
Copper sulfate: MBIT=20:1	25.3	138.81	103.17	134.55
Copper sulfate: MBIT=10:1	22.83	153.83	106.05	145.06
Copper sulfate: MBIT=1:1	19.67	178.55	133.26	133.98
Copper sulfate: MBIT=1:10	17.27	203.36	160.47	126.73
Copper sulfate: MBIT=1:15	17.2	204.19	162.36	125.76

The results (Table 11) showed that the effect of MBIT and copper sulfate on the control of tomato bacterial wilt was significantly improved, indicating that the two had a significant synergistic effect on the control of tomato bacterial wilt. In particular, the ratio of MBIT to copper sulfate is between 1:20 and 5:1, and the synergistic effect is obvious.

(12) MBIT and basic copper sulphate complex test for eggplant wilt virus

Table 12. Analysis of the results of the determination of the efficacy of MBIT and basic copper sulphate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Basic copper sulphate	48.56	100	/	/
MBIT	31.62	153.57	/	/
Basic copper sulphate: MBIT=30:1	38.08	127.52	101.73	125.35
Basic copper sulphate: MBIT=20:1	35.11	138.31	102.55	134.87
Basic copper sulphate: MBIT=10:1	31.54	153.96	104.87	146.81
Basic copper sulphate: MBIT = 1:1	28.01	173.37	126.79	136.74
Basic copper sulphate: MBIT = 1:10	25.66	189.24	148.7	127.27
Basic copper sulphate: MBIT = 1:15	26.44	183.66	150.22	122.26

The results (Table 12) showed that the effect of MBIT and basic copper sulphate on the control of eggplant wilt virus was significantly improved, indicating that the two have a significant synergistic effect on eggplant bacterial wilt, especially MBIT and basic copper sulphate. The ratio is between 1:20 and 15:1, and the synergistic effect is obvious.

(13) MBIT and cuprous oxide complex test for rice koji virus

Table 13. Analysis of the results of MBIT and cuprous oxide complex pairing rice koji

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Cuprous oxide	36.83	100	/	/
MBIT	22.51	163.62	/	/
Cuprous oxide: MBIT=30:1	28.51	129.18	102.05	126.59
Cuprous oxide: MBIT=20:1	25.64	143.64	103.03	139.42
Cuprous oxide: MBIT=10:1	22.22	165.75	105.78	156.69
Cuprous oxide: MBIT=1:1	18.93	194.56	131.81	147.61
Cuprous oxide: MBIT=1:10	18.07	203.82	157.84	129.13
Cuprous oxide: MBIT=1:15	18.93	194.56	159.64	121.87

The results (Table 13) showed that the effect of MBIT and cuprous oxide on the control of rice koji virus was significantly improved, indicating that the two had a significant synergistic effect on rice blast disease.

(14) MBIT and copper hydroxide complex paired rice bacterial stripe virus assay

Table 14. Analysis of the results of determination of bacterial spot streak virus in rice by MBIT and copper hydroxide

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper hydroxide	48.05	100	/	/
MBIT	32.69	146.99	/	/
Copper hydroxide: MBIT=30:1	36.8	130.57	101.52	128.62
Copper hydroxide: MBIT=20:1	33.97	141.45	102.24	138.35
Copper hydroxide: MBIT=10:1	30.18	159.21	104.27	152.69
Copper hydroxide: MBIT=1:1	27.12	177.18	123.5	143.46
Copper hydroxide: MBIT=1:10	24.97	192.43	142.72	134.83
Copper hydroxide: MBIT=1:15	25.68	187.11	144.05	129.89

The results (Table 14) showed that the effect of MBIT and copper hydroxide on the control of rice bacterial stripe virus was significantly improved, indicating that the two had a significant synergistic effect on rice bacterial leaf streak.

(15) MBIT combined with acacia and copper sulphate

Table 15. Analysis of the results of the determination of pepper anthrax virus by MBIT combined with succinic acid copper

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Amber	43.78	100	/	/
MBIT	25.36	172.63	/	/
Acrylate copper: MBIT=30:1	34.99	125.12	102.34	122.26
Acrylate copper: MBIT=20:1	32.3	135.54	103.46	131.01

Acrylate copper: MBIT=10:1	28.43	153.99	106.6	144.46
Acrylate copper: MBIT=1:1	23.76	184.26	136.32	135.17
Acrylate copper: MBIT=1:10	20.22	216.52	166.03	130.41
Aromatic acid copper: MBIT=1:15	20.85	209.98	168.09	124.92

The results (Table 15) showed that the effect of MBIT and succinated copper on the control of capsicum anthracis was significantly improved, indicating that the two had a significant synergistic effect on pepper anthracnose.

(16) MBIT and copper acetate complex pairing with lychee ulcer virus

Table 16. Analysis of the results of force determination of lychee ulcer virus by MBIT and copper acetate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper acetate	35.63	100	/	/
MBIT	32.03	111.24	/	/
Copper acetate: MBIT=30:1	27.67	128.77	100.39	128.27
Copper acetate: MBIT=20:1	26.29	135.53	100.58	134.75
Copper acetate: MBIT=10:1	24.15	147.54	101.11	145.92
Copper acetate: MBIT=1:1	24.88	143.21	106.12	134.95
Copper acetate: MBIT=1:10	twenty four	148.46	111.13	133.59
Copper acetate: MBIT=1:15	24.97	142.69	111.48	128.00

The results (Table 16) showed that the effect of MBIT combined with copper acetate on the control of litchi ulcer disease was significantly improved, indicating that the combination of the two has a significant synergistic effect on litchi ulcer disease.

(17) MBIT and copper octoate complexed against grape anthrax virus

Table 17. Analysis of the results of force determination of grape anthrax virus by MBIT and copper octoate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper octoate	33.56	100	/	/
MBIT	23.71	141.54	/	/
Copper octoate: MBIT=30:1	25.77	130.23	101.34	128.51
Copper octoate: MBIT=20:1	24.08	139.37	101.98	136.66
Copper octoate: MBIT=10:1	21.88	153.38	103.78	147.80
Copper octoate: MBIT=1:1	20.23	165.89	120.77	137.36
Copper octoate: MBIT=1:10	18.18	184.60	137.76	134.00
Copper octoate: MBIT=1:15	18.88	177.75	138.94	127.94

The results (Table 17) showed that the effect of MBIT and copper octoate on grape anthracnose was significantly improved, indicating that the two had a significant synergistic effect on grape anthracnose.

(18) MBIT and copper citrate complexed with cucumber bacterial keratosis

Table 18. Analysis of the results of determination of bacterial keratosis virus in cucumber with MBIT and copper citrate

The results (Table 18) showed that the effect of MBIT and copper citrate on the control of cucumber bacterial angular leaf spot was significantly improved, indicating that the two had a significant synergistic effect on cucumber bacterial angular leaf spot.

(19) MBIT and collateral copper complex pairing

Table 19. Analysis of the results of the determination of the efficacy of MBIT and collateral copper against tobacco wilt virus

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper ammonia	47.65	100	/	/
MBIT	33.93	140.44	/	/
Copper ammonia: MBIT=30:1	36.51	130.51	101.3	128.84
Copper ammonia: MBIT=20:1	34.08	139.82	101.93	137.17
Copper ammonia: MBIT=10:1	31.13	153.07	103.68	147.63
Copper ammonia: MBIT = 1:1	29.44	161.85	120.22	134.63
Copper ammonia: MBIT=1:10	26.52	179.68	136.76	131.38
Copper ammonia: MBIT=1:15	26.9	177.14	137.91	128.44

The results (Table 19) showed that the effect of MBIT and lycopene copper on the control of tobacco bacterial wilt was significantly improved, indicating that the two had a significant synergistic effect on tobacco bacterial wilt.

(20) MBIT combined with copper rosinate for the determination of cucumber anthrax virus

Table 20. Analysis of the results of the determination of cucumber anthrax virus by MBIT combined with copper rosinate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper rosinate	33.61	100	/	/
MBIT	25.87	129.92	/	/
Copper rosinate: BIT=30:1	27.19	123.61	100.97	122.42
Copper rosinate: MBIT=20:1	25.05	134.17	101.42	132.29
Copper rosinate: MBIT=10:1	22.79	147.48	102.72	143.57
Copper rosinate: MBIT=1:1	22.27	150.92	114.96	131.28
Copper rosinate: MBIT=1:10	20.83	161.35	127.2	126.85
Copper rosinate: MBIT=1:15	21.6	155.60	128.05	121.52

The results (Table 20) showed that the effect of MBIT and copper rosinate on the control of cucumber anthracnose was significantly improved, indicating that the two had a significant synergistic effect on cucumber anthracnose.

3, BBIT and organic copper or inorganic copper indoor activity test

(21) Determination of BBIT and copper sulfate complex with celery spot blotch virus

Table 21. Analysis of the results of the determination of celery virus by BBIT and copper sulfate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper sulfate	42.56	100	/	/
BBIT	27.15	156.76	/	/
Copper sulfate: BBIT=30:1	32.84	129.60	101.83	127.27
Copper sulfate: BBIT=20:1	29.91	142.29	102.7	138.55
Copper sulfate: BBIT=10:1	26.72	159.28	105.16	151.47
Copper sulfate: BBIT=1:1	23.99	177.41	128.38	138.19
Copper sulfate: BBIT=1:10	21.15	201.23	151.6	132.74
Copper sulfate: BBIT=1:15	21.41	198.79	153.21	129.75

The results (Table 21) showed that the control effect of BBIT and copper sulfate complexed with celery blight was significantly improved, indicating that the combination of the two had a significant synergistic effect on the control of celery blight.

(22) BBIT and basic copper sulphate complex test

Table 22. Analysis of the results of the determination of BBIT and basic copper sulphate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Basic copper sulphate	45.72	100	/	/
BBIT	33.65	135.87	/	/
Basic copper sulphate: BBIT=30:1	36.71	124.54	101.16	123.12
Basic copper sulfate: BBIT=20:1	34.45	132.71	101.71	130.48
Basic copper sulfate: BBIT=10:1	31.37	145.74	103.26	141.14
Basic copper sulphate: BBIT = 1:1	29.32	155.93	117.94	132.22
Basic copper sulphate: BBIT = 1:10	27.38	166.98	132.61	125.92
Basic copper sulphate: BBIT=1:15	28.06	162.94	133.63	121.93

The results (Table 22) showed that the effect of BBIT and basic copper sulphate on the control of lotus root virulence was significantly improved, indicating that the two had a significant synergistic effect on lotus seed blight.

(23) BBIT and cuprous oxide complex pairing strawberry white powder virus force test

Table 23. Analysis of the results of strawberry white powder virus determination by BBIT and cuprous oxide

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Cuprous oxide	26.52	100	/	/
BBIT	15.69	169.02	/	/
Cuprous oxide: BBIT=30:1	20.21	131.22	102.23	128.36
Cuprous oxide: BBIT=20:1	17.97	147.58	103.29	142.88
Cuprous oxide: BBIT=10:1	16.06	165.13	106.27	155.39
Cuprous oxide: BBIT=1:1	13.49	196.59	134.51	146.15
Cuprous oxide: BBIT=1:10	12.13	218.63	162.75	134.34
Cuprous oxide: BBIT=1:15	12.74	208.16	164.71	126.38

The results (Table 23) showed that the control effect of BBIT and cuprous oxide on strawberry white powder virus was significantly improved. It shows that the two have a significant synergistic effect on strawberry powdery mildew.

(24) BBIT and copper hydroxide complex pairing with lettuce downy mildew virus

Table 24. Analysis of the results of BBIT and copper hydroxide complex pairing of lettuce downy mildew virus

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper hydroxide	39.63	100	/	/
BBIT	26.85	147.60	/	/
Copper hydroxide: BBIT=30:1	30.75	128.88	101.54	126.92
Copper hydroxide: BBIT=20:1	28.71	138.04	102.27	134.97
Copper hydroxide: BBIT=10:1	25.79	153.66	104.33	147.29
Copper hydroxide: BBIT=1:1	22.63	175.12	123.8	141.46
Copper hydroxide: BBIT=1:10	21.2	186.93	143.27	130.48
Copper hydroxide: BBIT=1:15	21.75	182.21	144.63	125.98

The results (Table 24) showed that the control effect of BBIT and copper hydroxide complexed against the downy mildew of lettuce was significantly improved, indicating that the two had a significant synergistic effect on the downy mildew of lettuce.

(25) Determination of BBIT and humic acid copper complex with celery gray mold

Table 25. Analysis of the results of the determination of BBIT and succinated copper sulphate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Amber	32.15	100	/	/
BBIT	23.61	136.17	/	/
Acrylate copper: BBIT=30:1	25.87	124.28	101.17	122.84
Aromatic acid copper: BBIT=20:1	24.18	132.96	101.72	130.71
Aromatic acid copper: BBIT=10:1	20.9	153.83	103.29	148.93
Acrylate copper: BBIT=1:1	20.19	159.24	118.09	134.84
Aromatic acid copper: BBIT=1:10	19.17	167.71	132.88	126.21
Aromatic acid copper: BBIT=1:15	19.88	161.72	133.91	120.77

The results (Table 25) showed that the control effect of BBIT and succinated acid copper complex on celery gray mold was significantly improved, indicating that the two had a significant synergistic effect with celery gray mold.

(26) Determination of bacterial perforation virus of apricot by BBIT and copper acetate

Table 26. Analysis of the results of determination of bacterial perforation virus in apricot by BBIT and copper acetate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper acetate	43.55	100	/	/
BBIT	32.71	133.14	/	/
Copper acetate: BBIT=30:1	35.13	123.97	101.07	122.66
Copper acetate: BBIT=20:1	33.32	130.70	101.58	128.67
Copper acetate: BBIT=10:1	30.78	141.49	103.01	137.35
Copper acetate: BBIT=1:1	28.54	152.59	116.57	130.90
Copper acetate: BBIT=1:10	26.35	165.28	130.13	127.01

Copper acetate: BBIT=1:15

27.25

159.82

131.07

121.93

The results (Table 26) showed that the control effect of BBIT and copper acetate on the bacterial perforation of apricot was significantly improved, indicating that the combination of the two pairs of apricot bacterial perforation has a significant synergistic effect.

(27) BBIT and copper octoate complex determination of peach tree virus

Table 27. Analysis of the results of the determination of the efficacy of BBIT and copper octoate in peach tree ulcer virus

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper octoate	69.58	100	/	/
MBIT	41.81	166.42	/	/
Copper octoate: MBIT=30:1	52.29	133.07	102.14	130.28
Copper octoate: MBIT=20:1	48.91	142.26	103.16	137.90
Copper octoate: MBIT=10:1	43.75	159.04	106.04	149.98
Copper octoate: MBIT=1:1	38.73	179.65	133.21	134.87
Copper octoate: MBIT=1:10	33.23	209.39	160.38	130.56
Copper octoate: MBIT=1:15	34.13	203.87	162.27	125.63

The results (Table 27) showed that the effect of BBIT and copper octoate complexing on peach tree canker disease was significantly improved, indicating that the two had a significant synergistic effect on peach tree canker disease.

(28) BBIT and copper citrate complexed with onion creamy virus

Table 28. Analysis of the results of the determination of BBIT and copper citrate complexes

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper citrate	47.12	100	/	/
BBIT	34.97	134.74	/	/
Copper citrate: BBIT=30:1	38.32	122.96	101.12	121.60
Copper citrate: BBIT=20:1	36.44	129.31	101.65	127.21
Copper citrate: BBIT=10:1	33.91	138.96	103.16	134.70
Copper citrate: BBIT=1:1	31.57	149.26	117.37	127.17
Copper citrate: BBIT=1:10	28.45	165.62	131.58	125.87
Copper citrate: BBIT=1:15	29.58	159.30	132.57	120.16

The results (Table 28) showed that the control effect of BBIT and copper citrate on the onion downy mildew was significantly improved, indicating that the two pairs had a significant synergistic effect on onion downy mildew.

(29) BBIT and copper copper complex pairing cotton bacterial keratosis test

Table 29. Analysis of the results of bacterial keratosis test for BBIT and copper complex

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper ammonia	56.18	100	/	/
BBIT	30.71	182.94	/	/
Copper ammonia: BBIT=30:1	42.22	133.06	102.68	129.59
Copper ammonia: BBIT=20:1	38.77	144.91	103.95	139.40

Copper ammonia: BBIT=10:1	37.11	151.39	107.54	140.77
Copper ammonia: BBIT = 1:1	29.27	191.94	141.47	135.67
Copper ammonia: BBIT=1:10	25.17	223.20	175.4	127.25
Copper ammonia: BBIT=1:15	25.82	217.58	177.76	122.40

The results (Table 29) showed that the control effect of BBIT and lycopene copper on cotton bacterial leaf spot was significantly improved, indicating that the two had a significant synergistic effect on cotton bacterial leaf spot.

(30) Determination of Bacterial Leaf Virus Resistance of Cucumber with BBIT and Copper 6 Stearate

Table 30. Analysis of bacterial test results of bacterial bacterial leaf blight in combination with BBIT and copper rosinate

Pharmacy name	EC 50 (μg/ml)	ATI	TTI	Co-toxicity coefficient (CTC)
Copper rosinate	75.98	100	/	/
BBIT	39.36	193.04	/	/
Copper rosinate: BBIT=30:1	60.6	125.38	103	121.73
Copper rosinate: BBIT=20:1	57.74	131.59	104.43	126.01
Copper rosinate: BBIT=10:1	51.68	147.02	108.46	135.55
Copper rosinate: BBIT=1:1	40.46	187.79	146.52	128.17
Copper rosinate: BBIT=1:10	32.75	232.00	184.58	125.69
Copper rosinate: BBIT=1:15	33.57	226.33	187.23	120.88

The results (Table 30) showed that the control effect of BBIT and copper rosinate on cucumber bacterial leaf blight was significantly improved, indicating that the two had a significant synergistic effect on cucumber bacterial leaf blight.

(2) Field efficacy test

Test method: In the early stage of the disease, the first spray was immediately performed, and after 7 days, the second application was carried out, each treatment of 4 cells, 20 square meters per cell. The incidence of the disease was investigated before the drug and 11 days after the second drug. Each plot was randomly sampled at 5 points, and 5 crops were investigated at each point. The percentage of the lesion area per leaf on the whole plant was counted and graded. Disease index and control effect.

Expected control effect (%) = X + Y-XY / 100 (where X, Y is a single dose control)

Grading standards:

Level 0: no lesions;

Grade 1: less than 5 leaf lesions, less than 1 cm in length;

Grade 3: 6-10 leaf lesions, some lesions are longer than 1 cm;

Grade 5: 11-25 leaf lesions, some lesions are connected into pieces, and the lesion area accounts for 10-25% of the leaf area;

Grade 7: more than 26 leaf lesions, the lesions are connected into pieces, and the lesion area accounts for 26-50% of the leaf area;

Grade 9: The lesions are connected into pieces, and the area of the lesions accounts for more than 50% of the leaf area or the whole leaves are dead.

1. Field efficacy experiment of BIT and organic copper or inorganic copper pesticides

(1) Field efficacy test of BIT combined with copper sulfate in apple rot disease

Table 31 BIT and copper sulfate mixed with apple rot disease control effect

The results of the measurements (Table 31) show that the control effect of BIT combined with copper sulfate on apple rot disease is significantly improved, indicating that the two have a significant synergistic effect on apple rot.

(2) Field efficacy test of BIT mixed with basic copper sulphate for rice blast

Table 32 BIT combined with basic copper sulphate for controlling rice blast

The results of the determination (Table 32) show that the control effect of BIT combined with basic copper sulphate on rice blast is significantly improved, indicating that the two have a significant synergistic effect on rice blast.

(3) Field efficacy test of BIT mixed with cuprous oxide in Chinese cabbage rot disease

Table 33 BIT and cuprous oxide mixed with cabbage rot disease control effect

The results of the determination (Table 33) showed that the control effect of BIT and cuprous oxide on cabbage rot disease was significantly improved, indicating that the two had a significant synergistic effect on cabbage rot disease.

(4) Field efficacy test of BIT combined with copper hydroxide on tomato bacterial spot disease

Table 34 BIT and copper hydroxide mixed with tomato bacterial spot disease control effect

The results of the determination (Table 34) showed that the control effect of BIT combined with copper hydroxide on tomato bacterial spot disease was significantly improved, indicating that the two had a significant synergistic effect on tomato bacterial spot disease.

(5) Field efficacy test of BIT mixed with succinated acid and acid copper for bacterial perforation of peach tree

Table 35 BIT combined with succinated acid and acid copper to control the bacterial perforation of peach trees

The results of the determination (Table 35) showed that the control effect of BIT and succinated copper on the bacterial perforation of peach trees was significantly improved, indicating that the two had a significant synergistic effect on the bacterial perforation of peach trees.

(6) Field efficacy test of BIT mixed with copper acetate for citrus canker disease

Table 36 BIT combined with copper acetate to control the effect of citrus canker

The results of the determination (Table 36) showed that the control effect of BIT combined with copper acetate on citrus canker was significantly improved, indicating that the two had a significant synergistic effect on citrus canker disease.

(7) Field efficacy test of BIT mixed with copper octoate for bacterial leaf spot disease of cowpea

Table 37 BIT and copper ocyanate mixed with the control effect of cowpea bacterial angular leaf spot

The results of the determination (Table 37) showed that the control effect of BIT combined with copper octoate on the bacterial angular leaf spot of cowpea was significantly improved, indicating that the two had a significant synergistic effect on the bacterial keratosis of cowpea.

(8) Field efficacy test of BIT mixed with copper citrate for bacterial wilt of watermelon

Table 38 BIT combined with copper citrate to control the bacterial wilt of watermelon

The results of the determination (Table 38) showed that the control effect of BIT and copper citrate on the bacterial wilt of watermelon was significantly improved, indicating that the two had a significant synergistic effect on the bacterial wilt of watermelon.

(9) Field efficacy test of BIT mixed with lysine copper for mango bacterial spot disease

Table 39 BIT and complex ammonia copper mixed with mango bacterial spot disease control effect

The results of the determination (Table 39) showed that the control effect of BIT combined with lysine copper on mango bacterial spot disease was significantly improved, indicating that the two had a significant synergistic effect on mango bacterial spot disease.

(10) Field efficacy test of BIT mixed with copper rosinate for chrysanthemum bacterial angular leaf spot

Table 40 BIT combined with copper rosinate for the control effect of chrysanthemum bacterial angular leaf spot

The results of the determination (Table 40) showed that the control effect of BIT combined with copper rosinate on chrysanthemum bacterial angular leaf spot was significantly improved, indicating that the two have a significant synergistic effect on chrysanthemum bacterial leaf spot disease.

2, MBIT and organic copper or inorganic copper pesticide compound field efficacy experiment

(1) Field efficacy test of MBIT combined with copper sulfate for apple rot disease

Table 41 MBIT combined with copper sulfate to control apple rot disease

The results of the determination (Table 41) showed that the control effect of MBIT combined with copper sulfate on apple rot disease was significantly improved, indicating that the two had a significant synergistic effect on apple rot disease.

(2) Field efficacy test of MBIT mixed with basic copper sulfate for mango bacterial spot disease

Table 42 MBIT combined with basic copper sulfate to control the control of mango bacterial spot disease

The results of the determination (Table 42) showed that the control effect of MBIT mixed with basic copper sulfate on mango bacterial spot disease was significantly improved, indicating that the two had a significant synergistic effect on mango bacterial spot disease.

(3) Field efficacy test of MBIT mixed with cuprous oxide for jujube fruit disease

Table 43 MBIT and cuprous oxide mixed with jujube fruit disease control effect

The results of the determination (Table 43) showed that the control effect of MBIT combined with cuprous oxide on jujube fruit disease was significantly improved, indicating that the two combined with jujube fruit disease had significant synergistic effect.

(4) Field efficacy test of MBIT mixed with copper hydroxide for bacterial bacterial spot disease

Table 44 MBIT and copper hydroxide mixed with soybean bacterial spot disease control effect

The results of the determination (Table 44) showed that the control effect of MBIT combined with copper hydroxide on soybean bacterial spot disease was significantly improved, indicating that the two had a significant synergistic effect on soybean bacterial spot disease.

(5) Field efficacy test of MBIT combined with amber and copper sulphate

Table 45 MBIT combined with succinated acid and copper to control the effects of rice rot

The results of the determination (Table 45) showed that the control effect of MBIT combined with copper succinate and copper sulphate was significantly improved, indicating that the two had a significant synergistic effect on rice rot.

(6) Field efficacy test of MBIT combined with copper acetate in rice bacterial blight

Table 46 Effect of MBIT combined with copper acetate on the control of rice bacterial blight

The results of the determination (Table 46) showed that the control effect of MBIT combined with copper acetate on rice bacterial blight was significantly improved, indicating that the two had a significant synergistic effect on rice bacterial blight.

(7) Field efficacy test of MBIT combined with copper octoate for potato black shank

Table 47 MBIT combined with copper octoate to control the control effect of potato black shank

The results of the determination (Table 47) showed that the control effect of MBIT combined with copper octoate on potato black shank was significantly improved, indicating that the two had a significant synergistic effect on potato black shank.

(8) Field efficacy test of MBIT mixed with copper citrate in bacterial leaf spot of capsicum

Table 48 Effect of MBIT combined with copper citrate on the control of bacterial leaf spot in pepper

The results of the determination (Table 48) showed that the combination of MBIT and copper citrate was significantly improved in the control of bacterial leaf spot of capsicum, indicating that the two have a significant synergistic effect on the bacterial leaf spot of capsicum.

(9) Field efficacy test of MBIT mixed with copper ammonia to rice sheath blight

Table 49 Effect of MBIT and Lewis ammonia on the control of rice sheath blight

The results of the determination (Table 49) showed that the control effect of MBIT combined with lysine copper on rice sheath blight was significantly improved, indicating that the two had a significant synergistic effect on rice sheath blight.

(10) Field efficacy test of MBIT mixed with copper rosinate for bacterial perforation of peach tree

Table 50 MBIT combined with copper rosinate for the control of bacterial perforation in peach trees

The results of the determination (Table 50) showed that the control effect of MBIT combined with copper rosinate on the bacterial perforation of peach trees was significantly improved, indicating that the two had a significant synergistic effect on the bacterial perforation of peach trees.

3. Field efficacy experiment of BBIT and organic copper or inorganic copper pesticides

(1) Field efficacy test of BBIT mixed with copper sulfate in bacterial leaf spot of cauliflower

Table 51 Effect of BBIT combined with copper sulfate on the control of bacterial leaf spot in cauliflower

The results of the determination (Table 51) showed that the control effect of BBIT combined with copper sulfate on the bacterial keratosis of cauliflower was significantly improved, indicating that the two had a significant synergistic effect on the bacterial keratosis of cauliflower.

(2) Field efficacy test of BBIT mixed with basic copper sulfate to kiwifruit ulcer disease

Table 52 BBIT combined with basic copper sulfate to control the effect of kiwifruit ulcer

The results (Table 52) showed that the control effect of BBIT combined with basic copper sulfate on kiwi canker was significantly improved, indicating that the two had a significant synergistic effect on kiwifruit ulcer disease.

(3) Field efficacy test of BBIT combined with cuprous oxide for apple scab

Table 53 BBIT combined with cuprous oxide to control the effect of apple sore

The results of the determination (Table 53) showed that the control effect of BBIT combined with cuprous oxide on apple scab was significantly improved, indicating that the two had a significant synergistic effect on apple scab.

(4) Field efficacy test of BBIT mixed with copper hydroxide for bacterial bacterial hole disease in cherry

Table 54 BBIT and copper hydroxide mixed with cherry bacterial hole disease control effect

The results of the determination (Table 54) showed that the control effect of BBIT combined with copper hydroxide on cherry bacterial hole disease was significantly improved, indicating that the two had a significant synergistic effect on cherry bacterial hole disease.

(5) Field efficacy test of BBIT mixed with succinated copper and acid rot

Table 55 BBIT and arachidal acid copper mixed with pear bacterial flower rot control effect

The results of the determination (Table 55) showed that the control effect of BBIT and succinated copper on the bacterial flower rot of pear was significantly improved, indicating that the two had a significant synergistic effect on the bacterial flower rot of pear.

(6) Field efficacy test of BBIT mixed with copper acetate for garlic leaf spot

Table 56 BBIT combined with copper acetate to control the effect of garlic leaf spot

The results of the determination (Table 56) show that the control effect of BBIT combined with copper acetate on garlic leaf spot is significantly improved. The combination of garlic leaf spot has a significant synergistic effect.

(7) Field efficacy test of BBIT combined with copper octoate in apple flower rot

Table 57 BBIT combined with copper octoate to control apple flower rot

The results (Table 57) showed that the control effect of BBIT combined with copper octoate on apple flower rot was significantly improved, indicating that the two had a significant synergistic effect on apple flower rot.

(8) Field efficacy test of BBIT combined with copper citrate for strawberry bacterial wilt

Table 58 BBIT and copper citrate mixed with strawberry bacterial wilt control effect

The results of the determination (Table 58) showed that the control effect of BBIT combined with copper citrate on strawberry bacterial wilt was significantly improved, indicating that the two had a significant synergistic effect on strawberry bacterial wilt.

(9) Field efficacy test of BBIT combined with copper ammonia in the treatment of bacterial bacterial spot disease

Table 59 Effect of BBIT and Lewis ammonia mixed with soybean bacterial spot disease

The results of the determination (Table 59) showed that the control effect of BBIT combined with lysine copper on soybean bacterial spot disease was significantly improved, indicating that the two had a significant synergistic effect on soybean bacterial spot disease.

(10) Field efficacy test of BBIT mixed with copper rosinate in rice bacterial blight

Table 60 MBIT combined with copper rosinate for the control of rice bacterial blight

The results of the determination (Table 60) showed that the control effect of BBIT combined with copper rosinate on rice bacterial blight was significantly improved, indicating that the two had a significant synergistic effect on rice bacterial blight.

Claims (10)

1. A bactericidal composition, characterized in that the composition comprises two active components A and B, wherein the active component A is a structural compound having the formula (I), and the active component B is selected from the group consisting of inorganic copper. Or one of the organic copper bactericides, the weight ratio between the two components is 1:30 to 15:1,

   

   In the formula (I), R is selected from H or a C ₁ - C ₈ alkyl group; and in the active component B, the organic copper bactericide is a copper-containing complex.
2. The bactericidal composition according to claim 1, wherein in the active component B, the inorganic copper bactericide is selected from the group consisting of copper sulfate, basic copper sulfate, cuprous oxide or copper hydroxide, and organic copper sterilization The agent is selected from the group consisting of copper succinate, copper acetate, copper octoate, copper ruthenate, copper amide or copper rosinate.
3. The bactericidal composition according to claim 1, wherein in the formula (I), R is selected from H or a C ₁ - C ₄ alkyl group.
4. The bactericidal composition according to claim 3, wherein in the formula (I), R is selected from H, -CH ₃ or -C ₄ H ₉ , and the corresponding active component A is 1,2-benzox. Isothiazolin-3-one, 2-methyl-1,2-benzisothiazolin-3-one or 2-butyl-1,2-benzisothiazolin-3-one.
5. The bactericidal composition according to claim 1, wherein the weight ratio of the active component A to the active component B is from 1:20 to 10:1.
6. The bactericidal composition according to claim 5, wherein the weight ratio of the active component A to the active component B is from 1:20 to 1:1.
7. The bactericidal composition according to any one of claims 1 to 6, wherein the composition is made into a pesticide-acceptable dosage form from an active ingredient and an agrochemical adjuvant or an auxiliary.
8. The bactericidal composition according to claim 7, wherein the dosage form is a wettable powder, a suspension or a water-dispersible granule.
9. The bactericidal composition according to claim 7, wherein the pesticide adjuvant or adjuvant is selected from the group consisting of a carrier, a solvent, a dispersing agent, a wetting agent, an adhesive, a thickener, a binder, a surfactant or a fertilizer. One or several of them.
10. Use of the bactericide composition according to any one of claims 1 to 6 for controlling crop diseases in the agricultural field.