WO2017069717A1

WO2017069717A1 - A biofertilizer formulation

Info

Publication number: WO2017069717A1
Application number: PCT/TR2016/050390
Authority: WO
Inventors: Ibrahim ISILDAK; Azade ATTAR; Fatih ERCI
Original assignee: Isildak Ibrahim
Priority date: 2015-10-20
Filing date: 2016-10-20
Publication date: 2017-04-27
Also published as: TR201513059A1

Abstract

The invention is related to a solid form biofertilizer formulation comprising nutrients which contribute to plant growth and at least one of microorganism types of Azotobacter which can perform nitrogen fixation and/or of Acetobacter and/or Paenibacillus and/or Bacillus which can provide dissolved phosphate and potassium and/or of Pseudomonas and/or Streptomyces which can synthesize plant growth hormone and regulators and/or of Ensifer which can perform aflatoxin control.

Description

DESCRIPTION

A BIOFERTILIZER FORMULATION

Related Technical Field of the Invention

This invention is related to development of microbial based, ecologic biofertilizer formulations that aim easy transportation and application on the purpose of providing/increasing plant development.

Prior Art

At present, a great number of chemical fertilizers in the form of liquid, solid, powder or tablet have been developed. Chemical fertilizers damage the nature, change the environmental natura/flora because of their contents. In our country (Turkey) and in the world; the amount of organic substance in the agricultural soils is decreasing gradually due to the reasons of the unconscious use of the chemical fertilizers over the years, incorrect soil cultivation, single product culture, erosion etc. and 5% nutrient amount which is necessary to be in 0-40 cm of soil layer from which plants benefit decreases to the level of 1%. Biofertilizer formulations are developed with biotechnological methods for making soil close to its natural structure by solving adverse events in the short term, which decreasing agricultural lands against increasing population and incorrect applications create in the soil texture.

Biofertilizers are formulations comprising microorganisms which play role in taking nutrition elements necessary for plant growing from the soil for the purpose of usage in the agricultural production. These formulations are used in the cultivated areas by giving them directly to the soil or mixing them with the irrigation water or placing them with seeds.

The biofertilizer formulations used at the state of the art comprise generally microorganisms in the dormant state and various additive agents and mostly liquid formulations are improved and brought into use. When the lid of the biofertilizer used in liquid form is opened, it is required to be used without waiting. Otherwise, effectiveness of the biofertilizer becomes limited. That' s why, there are difficulties in the transportation and application issues. Conventional liquid form of biofertilizers offer solution for only one or some of plant needs such as nitrogen, potassium, phosphorus and growth hormone. There is not a single formula from which most of or all of these needs are met. This situation provides limited solution for plant nutrition. It is known that limited number of biofertilizers which are used in the form of tablet or solid like powder at the state of the art and which comprises different microorganisms are not full efficient for plant nutrition and economic.

In the patent document with the number of CN101870606 at the state of the art, a chemical fertilizer in the tablet form which does not have any microorganism content is mentioned.

In the patent document with the number of WO2013098856 at the state of the art, a fertilizer comprising an azotobacter microorganism and worm is mentioned.

In the patent documents with the numbers of KR20020087652(A) and KR20000000219(A) at the state of the art, the biofertilizers in the form of solid crushed with pressure and tablet which do not have a certain microorganism composition, however; which comprise some microorganisms selected randomly are mentioned.

Brief Description of the Invention

The aim of the invention is to realize a biofertilizer formulation in solid form which is suitable to be used in the grounded agriculture and greenhouse cultivation activities, especially in hydroponic greenhouses and pot cultivation; which comprises lyophilized bacteria mixture, mycorrhizal mushroom and nutrients.

The disclosed biofertilizer formulation of the invention comprises nutrients which contribute to plant growth, at least one of Azotobacter type microorganisms which can perform nitrogen fixation, of Acetobacter and/or Paenibacillus and/or Bacillus type microorganisms which can provide dissolved phosphate and potassium, of Pseudomonas and/or Streptomyces type microorganisms which can synthesize plant growth hormone and regulators and/or of Ensifer type microorganisms which can perform aflatoxin control.

The disclosed solid form biofertilizer of the invention comprises Azotobacter chroococcum (e.g. DSM 2286) as a type of Azotobacter which can perform nitrogen fixation. The disclosed biofertilizer of the invention comprises Acetobacter aurantius (e.g. DSM 6220) as a type of Acetobacter which can provide that the potassium salts in the plant cultivation medium are mobilized.

The disclosed biofertilizer of the invention comprises Bacillus megaterium var. phosphaticum (e.g. DSM 3228) as a type of Bacillus which can provide phosphorus salts to be dissolved. The disclosed biofertilizer of the invention comprises Streptomyces gelaticus (e.g. A381) as a type of Streptomyces which can produce growth regulator hormone for plants as a secondary metabolism product.

The disclosed biofertilizer of the invention comprises Ensifer meliloti (e.g. A404) as a type of Ensifer which can perform biological control of the Aflatoxin contamination.

The disclosed biofertilizer of the invention comprises Pseudomonas striata (e.g. DSM 3263) as a type of Pseudomonas which can control the plant growth by secreting indoleacetic acid which is a plant growth hormone.

The disclosed biofertilizer of the invention comprises mycorrhizal mushroom which supports the plant by living in symbiosis with it. In this relationship, the mycorrhizal mushroom takes carbon from the plant and contributes to the plant by transporting nutrient and water.

The disclosed solid form biofertilizer of the invention comprises lyophilized microorganism in order to be able to provide converting microorganisms into solid form and hiding of them without losing their liveliness in a certain time. In an embodiment of the disclosed biofertilizer formulation of the invention, it comprises microorganisms at equal concentrations.

The disclosed biofertilizer formulation of the invention comprises macro and micro nutrients.

Said nutrients are selected among ions such as K⁺, N0₃ ^", NH₄ ⁺, P0₄ ^3", S0₄ ^2", Mg²⁺, Ca²⁺, BO3^" , Fe²⁺, Mn²⁺, Zn²⁺, Cu²⁺, Mo²⁺ and Co²⁺ ions. The disclosed biofertilizer formulation of the invention comprises nutrients more than 25% and microorganisms more than 10⁷ cfu (colony forming unit).

The disclosed solid form biofertilizer of the invention comprises solid form porous structured biopolymer material which has protective purpose and which provides that microorganisms and nutrients are held together. In an embodiment of the disclosed solid form biofertilizer of the invention, it comprises nutrients in amounts of 84 mg of N (in the form of NO3^" and NH⁴⁺), 78 mg of K⁺, 168 mg of Ca²⁺, 46.5 mg of P (in the form of P0₄ ^3"), 32 mg of S (in the form of S0₄ ^"2), 48 mg of Mg ²⁺, 0.25 mg of B (in the form of B0₃ ^~), 1.5 mg of Fe²⁺, 0.25 mg of Mn²⁺, 0.125 mg of Zn²⁺, 0.015 mg of Cu , 0.025 mg of Mo and 0.01 mg of Co per gram together with the lyophilized microorganism mixture at the concentration of 10⁷ cfu/kg for ornamental plants (e.g. for tulip and clove).

In an embodiment of the disclosed solid form biofertilizer of the invention, it comprises nutrients in amounts of 105 mg of N (in the form of N0₃ ^" and NH⁴⁺), 117.5 mg of K⁺, 100 mg of Ca²⁺, 15.5 mg of P (in the form of P0₄ ^3"), 41 mg of S (in the form of S0₄ ^"2), 24 mg of Mg ²⁺, 0.25 mg of B (in the form of BO3^"), 1.5 mg of Fe²⁺, 0.25 mg of Mn²⁺, 0.05 mg of Zn²⁺, 0.015 mg of Cu²⁺, 0.015 mg of Mo²⁺ and 0.005 mg of Co²⁺ per gram together with the lyophilized microorganism mixture at the concentration of 10⁷ cfu/kg for vegetables (e.g. for tomato and cucumber).

In the disclosed biofertilizer formulation of the invention, the lyophilized microorganisms and other nutrients are pressed in crystallized form and are embedded in the porous biopolymer. Therefore, holding the microorganisms and nutrients in the biofertilizer together is provided and the biofertilizer is provided with a solid form. In the embodiments which have pellet or tablet form as solid form, the biofertilizer is also coated with a biopolymeric thin film such that it becomes a capsule.

The disclosed biofertilizer of the invention may be in the form of powder, granule, pellet or tablet.

In an embodiment of the disclosed solid form biofertilizer formulation of the invention, it comprises Azotobacter, Acetobacter, Paenibacillus, Bacillus, Pseudomonas, Streptomyces and Ensifer type microorganisms together for increasing the plant weight, root growth and stem height of plant. In the said embodiment, these microorganisms are Azotobacter chroococcum (DSM 2286), Acetobacter aurantius (DSM 6220), Paenibacillus mucilaginosus (DSM 24461), Bacillus megaterium var. phosphaticum (DSM 3228), Streptomyces gelaticus (A381), Ensifer meliloti (A404) and Pseudomonas striata (DSM 3263).

In an embodiment of the disclosed solid form biofertilizer formulation of the invention, it comprises Azotobacter, Streptomyces and Ensifer type microorganisms and mycorrhizal mushroom together for providing root growth in the plant. In this embodiment; Azotobacter chroococcum (DSM 2286), Streptomyces gelaticus (A381), Ensifer meliloti (A404) and mycorrhizal mushroom are used together for providing root growth in the plant. In another embodiment of the disclosed solid form biofertilizer formulation of the invention, it comprises Azotobacter, Acetobacter, Streptomyces and mycorrhizal mushroom for increasing plant weight and stem height of the plant. In this embodiment; Azotobacter chroococcum (DSM 2286), Acetobacter aurantius (DSM 6220), Streptomyces gelaticus (A381) and mycorrhizal mushroom are used.

Detailed Description of the Invention

The disclosed solid form biofertilizer formulation of the invention comprises macro and micro nutrients and microorganisms which contribute to plant growth, perform nitrogen fixation, provide dissolved phosphate and mobilized potassium, synthesize plant growth hormone and regulators and perform aflatoxin control.

The disclosed solid form biofertilizer formulation of the invention comprises also a thin polymeric film coating material on the purpose of protection of solid form and biopolymer which provides holding microorganisms and nutrients the biofertilizer comprises together.

The solid form may be powder, granule, pellet or tablet. The microorganisms in the content of the disclosed solid form biofertilizer of the invention which comprises microorganisms and nutrients are selected among the strains of Azotobacter and/or Acetobacter and/or Paenibacillus and/or Bacillus and/or Streptomyces and/or Ensifer and/or Pseudomonas.

The disclosed solid form biofertilizer of the invention comprises Azotobacter chroococcum (e.g. DSM 2286) as a type of Azotobacter.

The disclosed solid form biofertilizer of the invention comprises Acetobacter aurantius (e.g. DSM 6220) as a type Acetobacter.

The disclosed solid form biofertilizer of the invention comprises Paenibacillus mucilaginosus (e.g. DSM 24461) as a type of Paenibacillus. The disclosed solid form biofertilizer of the invention comprises Bacillus megaterium var. phosphaticum (e.g. DSM 3228) as a type of Bacillus.

The disclosed solid form biofertilizer of the invention comprises Streptomyces gelaticus (e.g. A381) as a type of Streptomyces. The disclosed solid form biofertilizer of the invention comprises Ensifer meliloti (e.g. A404) as a type of Ensifer.

The disclosed solid form biofertilizer of the invention comprises Pseudomonas striata (e.g. DSM 3263) as a type of Pseudomonas. The disclosed solid form biofertilizer of the invention comprises a mycorrhizal mushroom.

The nitrogen fixation is provided via Azotobacter chroococcum (DSM 2286) which is of the microorganisms in the content of the disclosed biofertilizer of the invention. Also, Azotobacter chroococcum inhibits the growth of some soil fungi such as Aspergillus, Fusarium, Curvularia, Alternaria, Helminthosporium, Fusarium by producing antifungal agents and suppresses harmful ones.

The potassium salts in the plant cultivation medium transform into the form the plant can get by being mobilized with Acetobacter auranitus (DSM 6220) and Paenibacillus mucilaginosus (DSM 24461); phosphorus and its salts also transform into the form the plant can get by being dissolved with Bacillus megaterium var. phosphaticum (DSM 3228). Streptomyces gelaticus (A381) which is of the microorganisms in the content of the disclosed biofertilizer of the invention secretes growth regulator for the plant as the secondary metabolism product. In addition; the secondary metabolism product has cyclo (prolyltyrosyl), diketopiperazine, antibiotic and pesticidal properties.

Ensifer meliloti (A404) which is of the microorganisms in the content of the disclosed biofertilizer of the invention performs the biological control of aflatoxin contamination.

Aflatoxin accumulation in the plants has harmful effects on humans, that's why it is necessary to provide keeping this substance under control also in the plants. The biological control of this substance is provided by Ensifer meliloti bacteria which the disclosed solid form biofertilizer of the invention comprises. Pseudomonas striata (DSM 3263) controls the plant growth by secreting indole acetic acid which is a plant growth hormone.

The mycorrhizal mushroom supports the plant by living in symbiosis with it and transports water and nutrients which the mushroom gets from soil to the plant. The microorganisms in the content of the disclosed solid form biofertilizer of the invention generally;

• Meet the nitrogen need of the plants by providing atmospheric nitrogen fixation,

• Transform phosphorus and potassium salts in the soil by dissolving them into the form that the plant can get,

• Secrete the plant growth hormones and regulators,

• Remove harmful compounds in the plant cultivation medium by splitting them,

• Suppressing the microorganisms which may prevent the plant growth with their biopesticidal effect,

· Perform the duties of improvement and protection of the natural, chemical and biological structure of the soil because they increase the organic content in the medium,

• Provide aeration of the soil and increase its water holding capacity,

• Decrease or remove the need for chemical fertilizer.

In an embodiment of the disclosed solid biofertilizer formulation of the invention, the microorganisms have equal concentrations.

The nutrients in the content of the disclosed solid biofertilizer of the invention may consist of different chemical salts comprising ions of K⁺, N0₃ ^", NH₄ ⁺, P0₄ ^3", S0₄ ^2", Mg²⁺, Ca²⁺, B0₃ ^", Fe²⁺, Mn²⁺, Zn²⁺, Cu²⁺, Mo²⁺ and Co²⁺.

The nutrients which the disclosed solid form biofertilizer of the invention comprises may change according to the environmental conditions or plant species.

In the disclosed solid form biofertilizer formulation of the invention, the nutrient content is more than 25% and the microorganism level is more than 10⁷ cfu/kg (colony forming unit).

In the invention, at least 10⁷ cfu/L of microorganism is transformed into the solid form from liquid state by the lyophilization process and the microorganism in its equivalent amount is used in the solid form.

In the disclosed solid form biofertilizer formulation of the invention, the microorganisms are lyophilized, pressed with the other nutrients in crystallized form and embedded in a porous polymer and therefore, the biofertilizer gains solid form. In an embodiment of the disclosed solid form biofertilizer of the invention which comprises the microorganisms and nutrients, the nutrients the biofertilizer comprises together with the lyophilized microorganism mixture in 10⁷ cfu/kg concentration are shown in Table 1 below for ornamental plants (e.g. for tulip and clove) and for vegetables (e.g. tomato and cucumber). Table 1. The amounts of nutrients used in the content of the solid form biofertilizer according to the plant species in an embodiment of the invention

In the said embodiment of the disclosed solid form biofertilizer of the invention comprising microorganisms and nutrients, the number of the living microbial cells was formulated in concentration of 10⁷ cfu/kg at least.

When it is applied to the growth medium, almost all of the used microorganisms, namely; almost the amount of 10⁷ cfu/kg may have the living cell concentration.

The mycorrhizal mushrooms in the content of the invention are found as spores.

Being in the solid form of the disclosed biofertilizer of the invention provides it to be able to be used by adding into the irrigation water or by leaving directly into the plant cultivation medium. The disclosed solid form biofertilizer formulation of the invention is suitable to be used in the grounded agriculture, greenhouses, pot cultivation and hydroponic production.

The disclosed solid form biofertilizer of the invention comprising microorganisms and nutrients contains microorganisms supporting plant growth, 16 essential elements of plant development and minerals refreshing soil; so it has the properties of multi-functional soil regulator and, microbial and organic fertilizer.

The disclosed solid form biofertilizer of the invention comprising microorganisms and nutrients is biodegradable and biocompatible. The microorganisms used in its content are not pathogen.

The microorganisms in the content of the disclosed solid form biofertilizer of the invention are in lyophilized form in the main embodiment of the invention. Thus, the microorganisms can be kept for a time period nearly at least 2 years without losing their liveliness.

In the soils where the solid form biofertilizer which is the subject matter of the invention is used; microorganisms reproduce, natural resistance forces of the plants increase, the chemical struggle medium transforms into the biological struggle medium and the natural balance is reestablished.

Due to the solid state structure of the disclosed biofertilizer of the invention comprising microorganisms and nutrients pressed in a polymeric matrix, the nutrients and microorganisms that biofertilizer tablets enclose in themselves are released at a certain speed during dissolution and so controlled release occurs. Having solid form of the disclosed biofertilizer of the invention makes its transportation and application easier. This situation provides it to be applied safely and effectively in the plants grown in pot at home.

Example:

The results of the study performed on a tomato plant which is grown in the pot soil for 15 days are given in the table below. As a result of the study performed by changing the used microorganisms and/or mycorrhiza (MR) for 15 days; plant weights, stem heights, leaf color according to the shades of green and root growth have been evaluated. The used seedlings are in standard sizes and have approximately 5-12 cm of height, 2-4 cm of root diameter. The performed case study is on the purpose of examination of the effectiveness of different microorganism combinations and the used nutrients do not differ. All seedlings have been planted by using the same branded pot soils and same sized pots. The results present the effect of different microorganism combinations on the plant in comparison with the control group which does not comprise microorganism. 5 g of solid tablet biofertilizer has been used in the sample tests. The used solid form biofertilizer comprises the nutrients given in Table 1 and microorganisms in the combinations given in Table 2. The control group has been grown only with irrigation water.

Table 2: The microorganisms used in the content of the solid form biofertilizer in an embodiment of the invention

1. Azotobacter chroococcum (DSM 2286)

2. Acetobacter aurantius (DSM 6220)

3. Paenibacillus mucilaginosus (DSM 24461)

4. Bacillus megaterium var. phosphaticum (DSM 3228)

5. Streptomyces gelaticus (A381)

6. Ensifer meliloti (A404)

7. Pseudomonas striata (DSM 3263)

MR: Mycorrhiza

The invention is not limited with the disclosed embodiments above, a skilled person in the art can produce different embodiments of the invention easily. They should be evaluated within the scope of invention protection demanded with claims.

Claims

1. A solid form biofertilizer formulation comprising nutrients which contribute to plant growth and characterized by comprising at least one of microorganism types of Azotobacter which can perform nitrogen fixation and/or of Acetobacter and/or Paenibacillus and/or Bacillus which can provide dissolved phosphate and potassium and/or of Pseudomonas and/or Streptomyces which can synthesize plant growth hormone and regulators and/or of Ensifer which can perform aflatoxin control.

2. A solid form biofertilizer formulation according to claim 1 characterized by comprising

Azotobacter chroococcum (e.g. DSM 2286) as Azotobacter which can perform nitrogen fixation.

3. A solid form biofertilizer formulation according to claim 1 or 2 characterized by comprising Acetobacter aurantius (e.g. DSM 6220) as a type of Acetobacter which can provide mobilization of the potassium salts in the plant cultivation medium.

4. A solid form biofertilizer formulation according to claim 3 characterized by comprising Paenibacillus mucilaginosus (e.g. DSM 24461) as a type of Paenibacillus which can provide mobilization of the potassium salts in the plant cultivation medium.

5. A solid form biofertilizer formulation according to claim 4 characterized by comprising

Bacillus megaterium var. phosphaticum (e.g. DSM 3228) as a type of Bacillus which can provide dissolution of the phosphorus and its salts.

6. A solid form biofertilizer formulation according to claim 5 characterized by comprising

Streptomyces gelaticus (e.g. A381) as a type of Streptomyces which can produce growth regulator hormone for plant as a secondary metabolism product.

7. A solid form biofertilizer formulation according to claim 6 characterized by comprising

Ensifer meliloti (e.g. A404) as a type of Ensifer which can perform the biological control of aflatoxin contamination.

8. A solid form biofertilizer formulation according to claim 7 characterized by comprising

Pseudomonas striata (e.g. DSM 3263) as a type of Pseudomonas which can control plant growth by secreting indole acetic acid which is a plant growth hormone.

9. A solid form biofertilizer formulation according to claim 8 characterized by comprising mycorrhizal mushroom which supports the plant by living in symbiosis with it and transports water and nutrients which it gets from soil to the plant while it gets carbon from the plant.

10. A solid form biofertilizer formulation according to claim 1 characterized by comprising lyophilized microorganism to be able to provide transformation of the microorganisms into solid form and keeping them without losing their liveliness for a long time.

11. A solid form biofertilizer formulation according to claim 10 characterized by comprising microorganisms at equal concentrations.

12. A solid form biofertilizer formulation according to claim 1 characterized by comprising macro and micro nutrients.

13. A solid form biofertilizer formulation according to claim 12 characterized in that the nutrients are selected among the chemical salts of the ions of K⁺, N0₃ ^", NH₄ ⁺, P0₄ ^3", S0₄ ^2" , Mg²⁺, Ca²⁺, BO3-, Fe²⁺, Mn²⁺, Zn²⁺, Cu²⁺, Mo²⁺ and Co²⁺.

14. A solid form biofertilizer formulation according to claim 13 characterized by comprising more than 25% of nutrient content and more than 10⁷ cfu/kg (colony forming unit) of microorganism.

15. A solid form biofertilizer formulation according to claim 14 characterized by comprising nutrients in amounts of 84 mg of N (in the form of NO3^" and NH⁴⁺), 78 mg of K⁺, 168 mg of Ca²⁺, 46.5 mg of P (in the form of P0₄ ^3"), 32 mg of S (in the form of S0₄ ^"2), 48 mg of

Mg ²⁺, 0.25 mg of B (in the form of B0₃ ^~), 1.5 mg of Fe²⁺, 0.25 mg of Mn²⁺, 0.125 mg of Zn²⁺, 0.015 mg of Cu²⁺, 0.025 mg of Mo²⁺ and 0.01 mg of Co²⁺ per gram together with the lyophilized microorganism mixture at the concentration of 10⁷ cfu/kg for ornamental plants (e.g. for tulip and clove).

16. A solid form biofertilizer formulation according to claim 14 characterized by comprising nutrients in amounts of 105 mg of N (in the form of NO3^" and NH⁴⁺), 117.5 mg of K⁺, 100 mg of Ca²⁺, 15.5 mg of P (in the form of P0₄ ^3"), 41 mg of S (in the form of S0₄ ^"2), 24 mg of Mg ²⁺, 0.25 mg of B (in the form of BO3^"), 1.5 mg of Fe²⁺, 0.25 mg of Mn²⁺, 0.05 mg of Zn²⁺, 0.015 mg of Cu²⁺, 0.015 mg of Mo²⁺ and 0.005 mg of Co²⁺ per gram together with the lyophilized microorganism mixture at the concentration of 10⁷-10⁸ cfu/kg for vegetables (e.g. for tomato and cucumber).

17. A solid form biofertilizer formulation according to claim 1 characterized by comprising a thin polymer film coating material and a polymer matrix providing microorganisms and nutrients to be kept together and controlled release of them.

18. A solid form biofertilizer formulation according to claim 1 characterized by comprising

Azotobacter, Acetobacter, Paenibacillus, Bacillus, Pseudomonas, Streptomyces and Ensifer type microorganisms together for increasing the plant weight, root development and stem height of the plant.

19. A solid form biofertilizer formulation according to claim 18 characterized by comprising the microorganisms of Azotobacter chroococcum (DSM 2286), Acetobacter aurantius (DSM 6220), Paenibacillus mucilaginosus (DSM 24461), Bacillus megaterium var. phosphaticum (DSM 3228), Streptomyces gelaticus (A381), Ensifer meliloti (A404) and Pseudomonas striata (DSM 3263) together for increasing the plant weight, root development and stem height of the plant.

20. A solid form biofertilizer formulation according to claim 1 characterized by comprising the microorganisms of Azotobacter, Streptomyces, Ensifer and mycorrhizal mushroom together for providing root development in the plant.

21. A solid form biofertilizer formulation according to claim 20 characterized by comprising Azotobacter chroococcum (DSM 2286), Streptomyces gelaticus (A381), Ensifer meliloti (A404) and mycorrhizal mushroom together for providing root development in the plant.

22. A solid form biofertilizer formulation according to claim 1 characterized by comprising Azotobacter, Acetobacter, Streptomyces and mycorrhizal mushroom together for increasing plant weight and stem height of the plant.

23. A solid form biofertilizer formulation according to claim 22 characterized by comprising Azotobacter chroococcum (DSM 2286), Acetobacter aurantius (DSM 6220), Streptomyces gelaticus (A381) and mycorrhizal mushroom together for increasing plant weight and stem height of the plant.

24. A solid form biofertilizer formulation according to claim 1 characterized by having solid form as powder, granule, pellet or tablet.

25. A biofertilizer formulation production method for obtaining a solid form biofertilizer formulation according to claim 1 characterized by the steps of;

· pressing lyophilized microorganisms with the other nutrients in crystallized form,

• then, embedding in a porous polymer, and,

• controlled releasing and keeping together.

26. A biofertilizer formulation production method according to claim 25 wherein the lyophilized microorganism and porous polymer comprising other nutrients are coated with a thin film as capsule in the form of tablet or pellet.