WO2017062901A3 - Methods to isolate extracellular vesicles in biofluids for biomarker discovery and disease diagnosis - Google Patents

Methods to isolate extracellular vesicles in biofluids for biomarker discovery and disease diagnosis Download PDF

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Publication number
WO2017062901A3
WO2017062901A3 PCT/US2016/056172 US2016056172W WO2017062901A3 WO 2017062901 A3 WO2017062901 A3 WO 2017062901A3 US 2016056172 W US2016056172 W US 2016056172W WO 2017062901 A3 WO2017062901 A3 WO 2017062901A3
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WO
WIPO (PCT)
Prior art keywords
biofluids
methods
extracellular vesicles
disease diagnosis
biomarker discovery
Prior art date
Application number
PCT/US2016/056172
Other languages
French (fr)
Other versions
WO2017062901A2 (en
Inventor
Andy TAO
Anton ILYUK
Ning Bao
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Tymora Analytical Operations Llc
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Publication date
Application filed by Tymora Analytical Operations Llc filed Critical Tymora Analytical Operations Llc
Publication of WO2017062901A2 publication Critical patent/WO2017062901A2/en
Publication of WO2017062901A3 publication Critical patent/WO2017062901A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/16—Sterilization
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0681—Filter
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

EVs are connected to onset and progression of numerous diseases and can be used for their diagnosis. Methods and platform technology to isolate EVs and detect intravesicle phosphoproteins from biofluids are discussed. Secreted extracellular vesicles in biofluids are effectively isolated and phosphoproteins are extracted for detection and identification as biomarkers for disease diagnosis using antibodies, mass spectrometry, or other like methods.
PCT/US2016/056172 2015-10-07 2016-10-07 Methods to isolate extracellular vesicles in biofluids for biomarker discovery and disease diagnosis WO2017062901A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562238693P 2015-10-07 2015-10-07
US62/238,693 2015-10-07

Publications (2)

Publication Number Publication Date
WO2017062901A2 WO2017062901A2 (en) 2017-04-13
WO2017062901A3 true WO2017062901A3 (en) 2017-05-26

Family

ID=58488674

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2016/056172 WO2017062901A2 (en) 2015-10-07 2016-10-07 Methods to isolate extracellular vesicles in biofluids for biomarker discovery and disease diagnosis

Country Status (1)

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WO (1) WO2017062901A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11548002B2 (en) 2017-05-19 2023-01-10 The General Hospital Corporation Engineered nano-interfaces for microfluidic isolation of extracellular vesicles
CN110339874B (en) * 2019-06-21 2021-05-11 中国科学院上海微系统与信息技术研究所 Microfluidic device for exosome separation and surface protein detection and use method
KR20210125206A (en) * 2020-04-08 2021-10-18 건국대학교 산학협력단 Composition for detecting pesticide including extracellular vesicle and use thereof
CN113769806B (en) * 2021-10-21 2022-09-27 暨南大学 Micro-fluidic chip, preparation method thereof and application of micro-fluidic chip in C-reactive protein detection by combining two-step microsphere competition method

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080160602A1 (en) * 2001-09-07 2008-07-03 Lin He Microcolumn-based, high-throughput microfluidic device
US20080231337A1 (en) * 2007-03-23 2008-09-25 University Of Southern California Compact subnanosecond high voltage pulse generation system for cell electro-manipulation
WO2010072410A2 (en) * 2008-12-23 2010-07-01 Universiteit Leiden Methods for immobilizing microvesicles, means and methods for detecting them, and uses thereof
WO2014107571A1 (en) * 2013-01-03 2014-07-10 Exosome Diagnostics, Inc. Methods for isolating microvesicles
US20140315195A1 (en) * 2011-05-24 2014-10-23 The Regents Of The University Of California Method for exosomal biomarker detection by electric field-induced release and measurement

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080160602A1 (en) * 2001-09-07 2008-07-03 Lin He Microcolumn-based, high-throughput microfluidic device
US20080231337A1 (en) * 2007-03-23 2008-09-25 University Of Southern California Compact subnanosecond high voltage pulse generation system for cell electro-manipulation
WO2010072410A2 (en) * 2008-12-23 2010-07-01 Universiteit Leiden Methods for immobilizing microvesicles, means and methods for detecting them, and uses thereof
US20140315195A1 (en) * 2011-05-24 2014-10-23 The Regents Of The University Of California Method for exosomal biomarker detection by electric field-induced release and measurement
WO2014107571A1 (en) * 2013-01-03 2014-07-10 Exosome Diagnostics, Inc. Methods for isolating microvesicles

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Publication number Publication date
WO2017062901A2 (en) 2017-04-13

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