WO2016009981A1 - Combination of harmful-arthropod-attracting compound and natural enemy - Google Patents

Combination of harmful-arthropod-attracting compound and natural enemy Download PDF

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Publication number
WO2016009981A1
WO2016009981A1 PCT/JP2015/069991 JP2015069991W WO2016009981A1 WO 2016009981 A1 WO2016009981 A1 WO 2016009981A1 JP 2015069991 W JP2015069991 W JP 2015069991W WO 2016009981 A1 WO2016009981 A1 WO 2016009981A1
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mites
methyl
natural enemies
compound
thrips
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PCT/JP2015/069991
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French (fr)
Japanese (ja)
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圭 河津
敦雄 水口
祐美子 野口
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協友アグリ株式会社
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Priority to JP2016534415A priority Critical patent/JPWO2016009981A1/en
Publication of WO2016009981A1 publication Critical patent/WO2016009981A1/en

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27Pseudomonas
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01MCATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M1/00Stationary means for catching or killing insects
    • A01M1/02Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
    • AHUMAN NECESSITIES
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    • A01N27/00Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
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    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/08Oxygen or sulfur directly attached to an aromatic ring system
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    • A01N33/00Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
    • A01N33/02Amines; Quaternary ammonium compounds
    • A01N33/08Amines; Quaternary ammonium compounds containing oxygen or sulfur
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    • A01N35/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/04Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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    • A01N37/44Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
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    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • A01N63/14Insects
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/10Animals; Substances produced thereby or obtained therefrom
    • A01N63/16Arachnids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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    • A01N63/22Bacillus
    • AHUMAN NECESSITIES
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    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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    • A01N63/38Trichoderma
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    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/40Viruses, e.g. bacteriophages

Definitions

  • the present invention synergistically combines an attracting compound for attracting harmful arthropods, and natural enemies against harmful arthropods in harmful arthropods, thereby preventing crops from harmful arthropod damage.
  • the present invention relates to protective control methods and predictive methods to protect, or combinations for use in these methods. Also, a method for controlling the behavior of harmful arthropod populations via such attracting compounds and natural enemies, especially for controlling and predicting the damage of plants by harmful arthropods in institutional greenhouses and in open fields, or Relates to a combination for use in these methods.
  • Damage to cultivated crops by harmful arthropods is a global problem. Some harmful arthropods also carry viruses, causing more serious damage to crops by causing viral diseases.
  • control of harmful arthropods that occur and cause damage to agricultural crops is often carried out with chemical pesticides.
  • minute harmful arthropods that inhabit closed places such as cormorants, it is difficult to detect at low density and control with chemical pesticides.
  • the control effect is lowered due to the emergence of resistance to chemical pesticides, and the environmental impact of the use of chemical pesticides is regarded as a problem. Therefore, there is a need for a method for detecting harmful arthropod populations and reducing the density of harmful arthropod populations in order to reduce damage to cultivated crops and plants.
  • Patent Document 1 In order to use host plants, harmful arthropods use volatile substances produced and released by plants. Accordingly, some of the plant-derived compounds act as attractants for harmful arthropods, and it is possible to attract harmful arthropods using an appropriate trap to which attractants are added (Patent Document 1).
  • Patent Document 2 Non-Patent Document 1, Non-Patent Document 2).
  • a substance produced by a certain organism is disadvantageous to the produced species, but kairomones are known as substances that have a beneficial effect on other species of organisms that have been received.
  • a substance produced by a plant and attracted by an accepted herbivore is a kairomone, and these substances are plant-derived kairomones.
  • Many of the substances found as attracting compounds are aromatic compounds known as plant-derived kairomones.
  • Many plant-derived kairomones have been reported as attractants of various harmful arthropods.
  • Plant-derived kairomones have been reported as attractants for thrips. For example, p-anisaldehyde, ethyl nicotinate, benzaldehyde, o-anisaldehyde, ⁇ -farnesene, eugenol, 3-phenylpropylaldehyde, monoterpenes (geraniol, linalool, nerol, citronellol, eucalyptol), monoterpene ester Class (isobornyl valerate, isobornyl pivalate, lavandulyl valerate) have been reported as attractants of Franklinella occidentalis, but the degree of attraction is different (Non-patent document 3, Non-patent document 4) Non-patent document 5, Non-patent document 6, Patent document 6, Patent document 7, Patent document 8).
  • Non-Patent Document 3 Non-patent document 7, Non-patent document 9).
  • a compound that exhibits kairomone-like activity but is not derived from a plant is sometimes referred to as an analog of a plant-derived kairomone.
  • ethyl nicotinate and methyl m-aminobenzoate are analogs of methyl nicotinate and methyl o-aminobenzoate, which are plant-derived compounds, respectively, and are not plant-derived compounds, but are attractants of thrips (Non Patent Literature 3, Non Patent Literature 5, Non Patent Literature 7, and Non Patent Literature 8).
  • the above substances may show different levels of attractiveness depending on the type of thrips, and have been reported to show species specificity.
  • natural enemies refer to organisms that function as predators or parasites within the biological factors involved in natural control, the power that controls the number of living organisms acting in nature, that is, natural control. In the invention, it is used as having the same concept. Furthermore, pathogenic bacteria and viruses that infect insects and kill directly or indirectly are natural enemies and are called natural enemy microorganisms.
  • Patent Document 10 A method for avoiding damage to crops by predating harmful arthropods has been performed (Patent Document 10). For example, cultivate wheat in a crop cultivation facility to infest parasitoids, then release the natural enemy Koleman abrabachi of wheat beetle and propagate it on the wheat to infest the crops in the cultivation facility. Generally used is a method of killing important harmful arthropods such as cotton aphids and peach aphids to prevent damage.
  • Patent Document 11 For example, in order to protect crops from thrips that are harmful arthropods, Kalanchoe, which is a suitable spawning plant of the common enemies, the domestic enemy, is cultivated inside or outside the crop cultivation facility or field, and the natural enemy There is a method of increasing the population density by increasing the number of the beetles, and effectively suppressing an increase in thrips of harmful arthropods (Patent Document 11).
  • Himehanakamushi (Orius genus) is known as an excellent natural enemy such as thrips, which are difficult-to-control harmful arthropods.
  • As a method for controlling such harmful arthropods Namihimehanamemushi, Tairikhimemekamemushi, etc. Has been tried (Patent Document 13).
  • Patent Document 13 in order to stably supply natural enemies, large-scale breeding indoors has resulted in a new problem that inbreeding weakness due to repeated inbreeding has occurred and the growth rate has decreased.
  • IP M Int eng r a te d P s M a a n e eng ent
  • the “push-pull method” as a pest management technique has been attracting attention in recent years.
  • the “push-pull method” is a decoy by planting corn and pigeons in cotton fields as “decoy crops” to control tobacco moths that harm cotton, and by applying neem oil that pests avoid. It was named because crops attracted (pull) pests and repellents kept pests away (Non-Patent Document 11).
  • the “push-pull method” as a pest management method is intended to control the distribution and density of insect populations in the field by combining factors that stimulate insect behavior such as localization, colonization, feeding, and spawning.
  • the stimulating factor is a factor showing a stimulating effect such as a chemical factor such as a plant-derived kairomone, an allomone, or an insect-derived pheromone, or a physical factor such as a visual stimulus, and a suppressor is a repellent or the like It is a factor that shows the repellent effect.
  • all the methods that combine “stimulating factors” and “suppressing factors” that control insect behavior are push-pull methods.
  • the push-pull method is different from the conventional methods in that the insect population is controlled by simultaneously using the acting factors of the stimulating factor and the inhibitory factor.
  • the push-pull method is a method to maintain the pest population at a low level by utilizing all the factors that control insect behavior and arranging them in the most appropriate design, and comprehensive pest management in a broad sense It can be said that technology.
  • a combination of a stimulating factor and an inhibitory factor works effectively depends on the interaction between the stimulating factor and the inhibitory factor and the position of the main crop. If the attractiveness of the stimulating factor is insufficient, the pest can be attracted to the adjacent main crop and cause damage. Alternatively, if the repellent nature of the suppressor is insufficient, the pests can invade the main crop and the damage to the main crop can be magnified. Thus, there is a problem that using a stimulation factor or a suppression factor by the push-pull method involves a certain risk.
  • an object of the present invention is to provide means for overcoming such disadvantages or problems.
  • the object is to provide at least one compound selected from the group consisting of compounds attracting harmful arthropods and at least one natural enemy selected from the group consisting of natural enemies against the harmful arthropods.
  • One aspect of the present invention is at least one compound selected from the group consisting of compounds having attractiveness to harmful arthropods and at least one compound selected from the group consisting of natural enemies against the harmful arthropods.
  • the place where treatment is required is not particularly limited as long as the desired effect can be achieved in accordance with the purpose of the present invention, but for example, it may be the same space such as in a facility cultivation house and an open field. it can. In this way, a specific attracting substance and a specific natural enemy are used in the same space at the same time to achieve an attraction effect that surpasses conventional techniques, so it is extremely useful for stable production of safe and high-quality agricultural products. It becomes possible to generalize a certain arthropod control method. Furthermore, the present invention is easy for farmers to accept and can minimize the use of chemically synthesized insecticides.
  • the present invention separately relates to a method including a method for attracting harmful arthropods using a compound that has been attracted to harmful arthropods already proposed in the art, and a harmful arthropod
  • the present invention relates to a combined use with a method including a method for repelling the harmful arthropod using natural enemies.
  • Such combined use is carried out in the same space in places where treatment of harmful arthropods is required, for example, methods for controlling harmful arthropods in institutional cultivation houses and outdoor areas, institutional cultivation houses and outdoor areas.
  • the timing of the implementation is not limited as long as the desired effect according to the object of the present invention can be achieved, but one of the methods is performed first, the other method is performed later, and the timing is mutually shifted. Or both may be performed simultaneously.
  • Examples of harmful arthropods to be treated in the method or combination of the present invention include, but are not limited to, for example, thrips, whiteflies, aphids, leafworms, stink bugs, butterflies, and Spider mites can be mentioned. Treatment has been used as a broad concept and generally means that the animal is handled so that an enhanced attraction response of the animal can be achieved.
  • “synergistic action” or “acting synergistically” means at least one compound selected from a specific compound having attraction to a specific harmful arthropod population and a specific harmful node. This means that the attraction effect when combining one or more specific natural enemies with a paw animal population at the same time is significantly higher than the expected effect (so-called additive effect) of the combination.
  • the attraction synergistic effect that is expected when one kind of attracting component compound and one natural enemy are used in combination is determined by the following Colby calculation formula.
  • the “mode of synergistically attracting harmful arthropods” referred to in the present invention is the same space in which the compound having the attractiveness to harmful arthropods and the natural enemy need their treatment. Means that the combination is selected so that the value exceeds the expected value of the attraction rate when calculated in the above equation. Thus, if one or more compounds selected from the group consisting of compounds having attraction to harmful arthropods can be used as the attracting substance as long as the above-mentioned synergistic action of attraction can be confirmed, Any combination of and falls within the scope of the present invention.
  • the compound used as an attracting substance used in the present invention may be any substance that is attractive to the target arthropod.
  • an attracting substance may be any substance that is attractive to the target arthropod.
  • the above attractant is not limited to using only one kind, and two or more kinds of compounds may be used in combination, and the ratio thereof may be any.
  • the combination of two kinds of compounds is not limited, but the weight ratio of the combination of two kinds of compounds is 0.0999 to 99.9001: 99.9001 to 0.0999, preferably It can be 0.1996 to 99.8004: 99.8044 to 0.1996, more preferably 0.99 to 90.01: 90.01 to 0.99.
  • the attractant may be used in combination of three or more compounds, and the ratio thereof may be any.
  • the combination of the three compounds is not limited, but the weight ratio of the combination of the three compounds is 0.0998 to 99.8004: 99.8044 to 0.0998: 0.0.0. 0998 to 99.8004, preferably 0.1992 to 99.6016: 99.616 to 0.1992: 0.1992 to 99.6016, more preferably 0.98039 to 98.03922: 98.03922 to 0.98039 : 0.98039 to 98.03922.
  • the attracting substances used in the present invention are all known compounds, and as these known compounds, commercially available compounds can be used as they are, obtained from commercially available preparations, or synthesized by a known method.
  • the attracting substance used in the present invention may be prepared by dissolving one or more compounds selected from the group of compounds having attraction as it is or by dissolving in an appropriate solvent such as hexane, xylene, acetone, dichloromethane, or the like. It is also possible to use this solution by impregnating it with a suitable carrier or holding body such as paper or cloth. It is also possible to formulate the two or more types of attracting active substances separately and use them in combination at the same location.
  • the attracting substance used in the present invention can efficiently kill harmful arthropods by using it as an attracting source in traps such as adhesive traps, corn traps, funnel traps, and basin traps.
  • the trap using the attracting substance of the present invention as an attracting source can also be used by installing it in a place where harmful arthropods are generated or flying, that is, indoors, facilities, or outdoors.
  • the trap may be used by coloring.
  • the combination of attractant and natural enemy of the present invention can also be used to predict or minimize damage to plants caused by harmful arthropods, or as an aid to such methods.
  • natural enemies used in the present invention include predatory natural enemies, parasitic natural enemies, natural enemy microorganisms, and the like.
  • the natural enemies are predatory natural enemies, such as Swallowsky spider mites (Amblyseius swirskii), Chilean spider mites (Phytoseiulius persimilis), Mite spider mites (Neuseilus caulifornicus), Ipterus tuna (Gypsum spp.) (Amblydromalus limonicus), Kumeliska burid mite (Amblyseius cucumeris), Nicellago burdock mite (Amblyseius esharais), Kousegekaburidani (Euseius sojaensis), Degenerance burdock mite (Ausbius bulgari) nerans), food moth gall midge (Aphidoletes aphidimyza), Thailand Riku Orius (Orius strigicollis), Harmoni
  • Flabus Bacillus simplex, Trichoderma atroviride, Pseudomonas fluorescens, Coniochilium mini It is preferable to contain tans (Coniothyrium minitans), zucchini yellow mosaic virus (Zucchini yellow mosaic virus), and Bacillus thuringiensis (Bacillus thuringiensis).
  • tans Coniothyrium minitans
  • zucchini yellow mosaic virus Zucchini yellow mosaic virus
  • Bacillus thuringiensis Bacillus thuringiensis
  • Cabbage mite (Neoseiulus californicus), Carp mite (Gyneesius liturivorus), Spider mite (Neoseiurus barkeri), Limonica calyx mite (Amblydromalus limonicus) Amblyseius cucumeris, Amblyseius eharai (Euseius sojaensis), Degenerance burdock mites (Amblyseius degenerans), Aphididae (Amblyseius degenerans) Harmonia axyridis, Agricultural thrips (Franklinthrips vespiformis), Akamegashiwaku thrips (Haplotrips brevitubus), Himeka nosago (Propylea japonica) diocoris tenuis, Pyrophorus typicus, yamasoku pheasant (Chrysoperla carnea),
  • the amount of natural enemies released to harmful arthropods used in the present invention is 0.01 to 200 / m 2 , preferably 0.1 to 100 / m 2 in the case of predatory or parasitic natural enemies, More preferably, it can be 1 to 50 heads / m 2 , and in the case of natural enemy microorganisms, 0.1 to 20 kg / ha, preferably 0.5 to 10 kg / ha, more preferably 1 to 5 kg / ha. Can do.
  • the biological control method has an environmental condition in which the growth rate of natural enemies used exceeds the growth rate of harmful arthropods.
  • an environmental condition for example, selecting a cultivation type of a crop adapted to a temperature condition included in a suitable temperature range for development of natural enemies, the temperature condition (specifically, for example, a temperature condition of a growth zero point or higher, It is possible to choose to set the temperature in the facility cultivation house to a temperature that is preferably higher than the flight limit temperature, more preferably higher than the egg-laying limit temperature, and the natural enemy's individual rather than the number of harmful arthropods. The natural increase rate of numbers should be exceeded.
  • information related to the growth zero of the natural enemies and harmful arthropods, the flight limit temperature, the egg-laying limit temperature, etc. are collected and accumulated in advance, and rather than the development of harmful arthropods based on the accumulated information.
  • Appropriate temperature conditions can be selected depending on the growth of the natural enemy.
  • methods for determining whether natural enemy growth rates have environmental conditions that exceed those of harmful arthropods may be empirical or quantitative.
  • Qualitative means as well as means may be used. For example, whether or not the above environmental conditions are met, and the number of natural enemies found per crop (for example, one or more in 10-20 flowers) is investigated and observed. , You may observe and confirm the increase of natural enemies that naturally occurred after the release of natural enemies.
  • this biological control method when releasing natural enemies, it is also preferable to release the natural enemies by releasing them several times, such as about 2 to 3 times, for example, at intervals of one week from the initial stage of occurrence of harmful arthropods.
  • the combination of the attracting substance and the natural enemy can exist by being applied to the facility house or the open ground that are the same space at the same time or in any desired order.
  • the combination of the attractant of the present invention and the natural enemy is based on at least one compound selected from the group consisting of compounds derived from plant-derived kairomones and analogs thereof described below, and natural enemies described below. It can be a combination with at least one natural enemy selected from the group consisting of: As a combination of attractants, 1) Swarsky burdock mites, p-anisaldehyde 2) Swarsky burdock mites, benzaldehyde 3) Swarsky burdock mites, ethyl nicotinate 4) Swarsky burdock mites, geraniol 5) Swarsky burdock mites, linalool 6) Swarsky burdock mites, Nellore 7) Swar 8) Swarsky burdock mite, o-anisaldehyde 9) Swarsky burdock mite, ⁇ -farnesene 10) Swarsky burdock mite, methyl anthran
  • Harmful arthropods can be investigated by counting the number of organisms captured in the trap with the attractant. Based on the estimated population density, the necessity for control is determined. Massive capture of harmful arthropods requires the method of harmful arthropod removal provided by the present invention. Conversely, if the harmful arthropod is of low density, it does not necessarily require rapid control.
  • the attractant of the present invention can be formulated as necessary and placed in a trap.
  • Such traps are designed to release an effective amount of attractant.
  • the trap is installed in a place where harmful arthropods are generated or expected to occur.
  • the odor of the attractant attracts harmful arthropods to the trap, and then, for example, by pre-installing an insecticide that shows a lethal effect on the harmful arthropods in the trap, It is possible to kill harmful arthropods attracted inside. Alternatively, it is possible to attract and kill by installing an adhesive plate in or near the trap. Examples of effective insecticidal active ingredients that can be combined with the attractant of the present invention are described below.
  • Effective insecticidal active ingredient o-ethyl o-4-nitrophenyl phenylphosphonothioate (EPN), acephate, isoxathion, isofenphos, isoprocarb, etrimfos, oxydeprofos, oxydeprofos (Quinalphos), cadusafos, chlorethoxyphos, chlorpyrifos, chlorpyrifos-methyl, chlorofenvinphos, alithifo Difofoton, dimethoate, sulprofos, diazinon, thiomethon, tetrachlorvinphos, tebupyrimfos, tebupirimfos, tebupirimfos vamdotion, pyraclophos, pyridafenthion, pyrimiphos-methyl, fenitrothion, fenthion, phenthate, buthothio
  • insecticidal active ingredients synergists (eg piperonyl butoxide, sesamex sulfoxide), MGK 264, N-decylimidazole, N-decylimidazole, Contains an inducer and a resistant (WARF-antiresistant), TBPT, TPP, IBP, PSCP, CH3I, t-phenylbutenone, diethyl maleate, DMC, FDMC, ETP, ETN
  • synergists eg piperonyl butoxide, sesamex sulfoxide
  • MGK 264 eg., N-decylimidazole
  • N-decylimidazole N-decylimidazole
  • TBPT TPP
  • IBP IBP
  • PSCP CH3I
  • t-phenylbutenone diethyl maleate
  • DMC FDMC
  • Examples of the solid carrier used in formulating the attractant include kaolin clay, attapulgite clay, bentonite, montmorillonite, acid clay, pyrophyllite, talc, diatomaceous earth and calcite, corn cob flour,
  • Examples include a natural organic material such as walnut shell powder, a synthetic organic material such as urea, a salt such as calcium carbonate and ammonium sulfate, a fine powder or a granular material composed of a synthetic inorganic material such as synthetic hydrous hydroxide
  • the liquid carrier includes, for example, xylene , Aromatic hydrocarbons such as alkylbenzene and methylnaphthalene, alcohols such as 2-propanol, ethylene glycol, propylene glycol and ethylene glycol monoethyl ether, ketones such as acetone, cyclohexanone and isophorone, vegetable oils such as soybean oil and cottonseed oil , Petroleum Aliphatic hydrocarbons, esters, di
  • Surfactants include, for example, anions such as alkyl sulfate esters, alkylaryl sulfonates, dialkyl sulfosuccinates, polyoxyethylene alkylaryl ether phosphate esters, lignin sulfonates and naphthalene sulfonate formaldehyde polycondensates.
  • Nonionic surfactants such as surfactants, polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl polyoxypropylene block copolymers and sorbitan fatty acid esters, and cationic surfactants such as alkyltrimethylammonium salts.
  • formulation adjuvants include, for example, water-soluble polymers such as polyvinyl alcohol and polyvinyl pyrrolidone, gum arabic, alginic acid and its salts, polysaccharides such as CMC (carboxymethyl cellulose) and xanthan gum, aluminum magnesium silicate, Examples include inorganic substances such as alumina sol, preservatives, colorants, and stabilizers such as PAP (isopropyl acid phosphate) and BHT.
  • water-soluble polymers such as polyvinyl alcohol and polyvinyl pyrrolidone
  • gum arabic alginic acid and its salts
  • polysaccharides such as CMC (carboxymethyl cellulose) and xanthan gum
  • aluminum magnesium silicate examples include inorganic substances such as alumina sol, preservatives, colorants, and stabilizers such as PAP (isopropyl acid phosphate) and BHT.
  • PAP isopropyl acid phosphate
  • the present invention is based on harmful arthropods (for example, harmful arthropods such as harmful insects and harmful ticks) that attract and capture harmful arthropods and cause feeding, sucking, etc. Plants can be protected from harm.
  • harmful arthropods for example, harmful arthropods such as harmful insects and harmful ticks
  • Plants can be protected from harm.
  • the prediction method and control method using the attractant and natural enemy of the present invention can be used in farmland such as fields, paddy fields, lawns, orchards, or non-agricultural land.
  • the present invention is used to control harmful arthropods in the farmland without causing any phytotoxicity in the farmland where the “plants” listed below are cultivated. Can do.
  • Agricultural crops corn (Zea mays), rice (Oryza sativa), wheat (Triticum aestivum), barley (Hordeum vulgare), rye (Secale cereal), oat (Avena sativa olh, sorghum) (Gossypium arboreum), soybean (Glycine max), peanut (Arachis hypogaea), buckwheat (Fagopyrum esculentum), sugar beet (Beta bulgaris ssp. Vulgaris), rape (rape. (Saccharum officinarum), tobacco (Nicotiana tabacum) and the like.
  • Vegetables Eggplant (Solanum melogena), tomato (Solanum lycopersicum), green pepper (Capsicum annuum var. Angulosum), capsicum (Capsicum annuum), potato (Solanum tubulus cucumber) , Pumpkin (Cucurbita maxima), zucchini (Cucurbita pepo), watermelon (Citullus lanatas), melon (Cucumis melo) etc., cruciferous vegetables (Raphanus sativus var. , Seyo Uwasabi (Armoracia rusticana), kohlrabi (Brassica oleracea var. Gongylodes), Chinese cabbage (Brassica rapa var.
  • Trees other than fruit trees tea (Camellia sinensis), mulberry (Morus alba), flowering trees (Rhodendroni indicum), camellia (Camellia japonica laca), hydrangea macrophylla (Hydrangea macrophylla) Cherry (Cerasus xyedoensis), Liriodendron tulipifera, Salgersberger (Lagerstroemia indica), Kinsokusei (Osmanthus fragrance var.
  • Aurantiacus) typhylla var.japonica dogwood (Benthamidia florida), eucalyptus (Eucalyptus globulus), ginkgo (Ginkgo biloba), lilac (Syringa vulgaris), maple (Acer palmatum), oak (Quercus myrsinaefolia), poplar (Populus angulata), Judas tree ( Cercis chinensis, Fuyu (Liquidambar formosana), Platanus (Platanus orientalis), Japanese zelkova (Zelkova serrata), Kurobe (Thuja standishii), Japanese cypress (Abies figas) ), Juniper (Juniperus rigida), pine (Pinus densiflora), spruce (Picea jezoensis var.
  • Lily Lily (Lilium spp.), Pansy (Viola X wittrocciana), Cyclamen (Cyclamen spp.), Orchidaceae spp., Lily of the valley (Convallaria majlis), Lavender (lavenda laval) oleracea var. acephala f. tricolor, primula (Primula spp. alt), poinsettia (Euphorbia pulcherrima), gladiolus spp., cattleya spm.
  • preferable examples include eggplant, tomato, pepper, capsicum, cucumber, pumpkin, zucchini, watermelon, melon, radish, turnip, cabbage, cabbage, mustard, broccoli, cauliflower, rape, burdock, garlic, artichoke, lettuce , Leek, onion, garlic, asparagus, carrot, parsley, celery, spinach, perilla, mint, basil, strawberry, sweet potato, yam and taro.
  • the “plant” may be a plant to which resistance is imparted by a genetic recombination technique or a breeding method by crossing.
  • a bulb means here a bulb, a bulb, a rhizome, a tuber, a tuberous root, and a root support body.
  • Examples of harmful arthropods for which the combined use of a harmful arthropod attractant and natural enemies according to the present invention exhibit a controlling effect include the following.
  • Species of the outer pods, Pteridopsis, Arthropoda Lalodelphax striatellas, Japanese planthoppers (Nilaparvata lugens, etc.), Japanese planthoppers (Sugatella furcifera), etc.
  • Cotton aphids (Aphis gossypii), peach aphids (Myzus persicae), radish aphids (Brevicoryne brassicae), tulip beetle aphids (Macrosiphum euphorbiae), potato aphid ni), wheat aphids (Rhopalosiphum padi), aphids such as the citrus aphid (Toxoptera citriticidus), Nezara antenatum (Shirasotemushishito), Riptortus (Liptortus) (Eysarcoris parvus), smelt beetle (Halyomorpha mista), stink bugs such as the finished plant bug (Lyus lineolaris), Trialureodes vapariorium, B.
  • Arctiidae such as Kashirohitori (Hyphantria cunea), clothes moth (Tinea translucens) Hirozukoga such as such; Species of Arthropoda: Hemimya antiqua, Hemimya platya, Amymyza oryzae roe, Ms.
  • the leaf fly (Dacus cucurbitae), the fruit fly (Ceratitis capitata) and the like; Inner ⁇ eyes Coleoptera arthropod pests: beetle, Epilachna vigintioctopunctata (Epilachna vigintioctopunctata), cucurbit leaf beetle (Aulacophora femoralis), Kisujinomihamushi (Phyllotreta striolata), Inedorooimushi (Oulema oryzae), rice weevil (Echinocnemus squameus), rice water weevil (Lissorhoptrus oryzophilus), Cotton weevil (Anthonomus grandis), Azuki beetle (Callosobrchuchus chinensis), Shibao weevil (Sphenophorus venatus), Japanese beetle (Popilia japonica), Doganebui
  • the harmful arthropod-inducing substance and natural enemies related to the present invention are preferably harmful arthropods that are effective in controlling the outer enemies.
  • harmful arthropods include upper-fly fly harmful arthropods, tick-eye harmful arthropods, and more preferably outer limbs, thrips-eye harmful arthropods.
  • Test Example 1 Attracting effect when individual attractants or natural enemies are treated p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, ⁇ -farnesene, methyl anthranilate, benzoic acid Selected from methyl acetate, o-aminoacetophenone, o-anisidine, methyl m-aminobenzoate, methyl o-toluate, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene, ⁇ -hexylcinnamaldehyde A hexane solution containing 1 ⁇ g of each single agent was prepared.
  • Test Example 2 Synergistic effect of attractant and natural enemy p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, ⁇ -farnesene, methyl anthranilate, methyl benzoate, o-aminoacetophenone Weight of 1 ⁇ g of each single agent selected from o-anisidine, methyl m-aminobenzoate, methyl o-toluylate, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene, ⁇ -hexylcinnamaldehyde A hexane solution containing was prepared.
  • Attracting rate (Number of catches in treated area ⁇ number of catches in untreated area) * 100 / (total number of released insects per cup)
  • the attraction synergistic effect expected when a given active ingredient compound and a natural enemy are simultaneously treated can be obtained from the following Colby calculation formula.
  • “Formula 2”; E X + Y ⁇ (X * Y) / 100
  • X Attraction rate when treating active ingredient compound A
  • Y Attraction rate when treating natural enemy B
  • E Attraction rate expected when active ingredient compound A and natural enemy B are treated simultaneously (expected attracting value)
  • Test Example 3 Attracting effect when individual attractants or predatory natural enemies are treated individually A hexane solution containing 1 ⁇ g of each single agent selected from p-anisaldehyde, nerol and squalene is prepared, and this solution is applied to filter paper. After the impregnation, the filter paper was attached to the central part of the colored adhesive plate installed in the plastic ice cream cup. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for the thrips. After 24 hours, the number of orange thrips captured in the colored sticky trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 3.
  • Test Example 4 Synergistic effect of attractant and predatory natural enemy A hexane solution containing 1 ⁇ g of each single agent selected from p-anisaldehyde, nerol and squalene was prepared. After this solution was impregnated into filter paper, it was attached to the central part of the colored adhesive plate installed in a plastic ice cream cup. At the same time, two adults of the mosquito bug were released in an ice cream cup. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green leaf pieces laid with absorbent cotton containing water were placed as food for the Thrips thrips.
  • Test Example 5 Attracting effect when individual attractants or predatory natural enemies are treated p-anisaldehyde, linalool, citronellol, o-anisaldehyde, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-toluic acid
  • a hexane solution containing 1 ⁇ g of each single agent selected from methyl, eugenol, cinnamaldehyde, eucalyptol, squalene, and ⁇ -hexylcinnamaldehyde was prepared, and this solution was impregnated into filter paper.
  • the filter paper was affixed to the central part of the colored adhesive plate installed in the cream cup.
  • Test Example 6 Synergistic effect of attractant and predatory natural enemy p-anisaldehyde, linalool, citronellol, o-anisaldehyde, methyl anthranilate, methyl benzoate, o-aminoacetophenone, methyl o-toluate, eugenol, cinnamaldehyde, A hexane solution containing 1 ⁇ g of each single agent selected from eucalyptol, squalene, and ⁇ -hexylcinnamaldehyde was prepared. After this solution was impregnated into filter paper, it was attached to the central part of the colored adhesive plate installed in a plastic ice cream cup.
  • Test Example 7 Attracting effect when individually treated with attractant or predatory natural enemy Prepare a hexane solution containing 1 ⁇ g of each single agent selected from o-anisaldehyde, methyl benzoate and o-aminoacetophenone, This solution was impregnated into filter paper. Place the absorbent cotton containing water in a plastic ice cream cup, place a colored sticky plate on top of this and the green leaf pieces as food for the spider mite, and place the filter paper containing the attracting compound in the center of the sticky plate Affixed. Thereafter, 15 nymph mites were released in the ice cream cup. After 24 hours, the number of spider mites captured in the colored adhesive trap was counted, and the attraction rate was calculated using Equation 1.
  • Test Example 8 Synergistic effect of attractant and predatory natural enemy A hexane solution containing 1 ⁇ g of each single agent selected from o-anisaldehyde, methyl benzoate and o-aminoacetophenone was prepared, and this solution was impregnated on filter paper. It was. Place the absorbent cotton containing water in a plastic ice cream cup, place a colored sticky plate on top of this and the green leaf pieces as food for the spider mite, and place the filter paper containing the attracting compound in the center of the sticky plate Affixed. At the same time, two adult dust mites were released in the ice cream cup, and then 15 nymph mites were released in the ice cream cup.
  • Test Example 9 Attracting effect when individual attractants or parasitic natural enemies are treated individually A hexane solution containing 1 ⁇ g of each single agent selected from ethyl nicotinate, linalool, citronellol, methyl benzoate and cinnamaldehyde was prepared.
  • the filter paper was impregnated with this solution. Place an absorbent cotton containing water in a plastic ice cream cup, place a colored sticky plate on top of this and a green leaf leaf as a aphid bait, and place an attracting compound containing an attracting compound in the center of the sticky plate The filter paper contained was affixed. Thereafter, 15 peach aphids were released in the ice cream cup.
  • Test Example 10 Synergistic effect of attractant and parasitic natural enemy Prepare a hexane solution containing 1 ⁇ g of each single agent selected from ethyl nicotinate, linalool, citronellol, methyl benzoate, and cinnamaldehyde, and impregnate the filter paper with this solution. I let you. Place the absorbent cotton containing water in a plastic ice cream cup, place a colored sticky board on top of this and a green leaf piece as food for aphids, and place the filter paper containing the attracting compound in the center of the sticky board. Affixed.
  • Test Example 11 Attracting effect when individual attracting compounds or natural enemy microorganisms were treated p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, methyl anthranilate, methyl benzoate, o-aminoacetophenone, Selected from o-anisidine, methyl m-aminobenzoate, methyl o-toluate, ⁇ -hexylcinnamaldehyde, o-anisaldehyde, ⁇ -farnesene, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene
  • a hexane solution containing 1 ⁇ g of each single attractant compound was prepared.
  • kidney beans were immersed in a solution of each natural enemy microorganism selected from currently commonly used natural enemy microorganisms diluted to each practical concentration and air-dried. Then, it cut
  • Attracting ratio to attracting compound (number of individuals attracted to treatment area ⁇ number of individuals attracted to control) * 100 / (number of tests)
  • Attraction rate to untreated leaf pieces (number of individuals attracted to untreated leaf pieces-number of individuals attracted to control) * 100 / (number of tests)
  • “Attracted by control” in the formula indicates that the leaf pieces not treated with either the attracting compound or the natural enemy microorganisms are attracted to the leaf pieces on one side when they are placed at both ends of the glass tube.
  • Test Example 12 Synergistic effect of attractant compound and natural enemy microorganisms p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-anisidine, m-amino
  • a hexane solution containing 1 ⁇ g weight was prepared.
  • the present invention it is possible to provide a method capable of effectively controlling harmful arthropods using the harmful arthropod-attracting compound and the harmful arthropod-controlling natural enemy.
  • a proposal method of a pest control system that is extremely useful for stably producing safe and high-quality agricultural products can be made into a general-purpose technology.
  • the invention makes it possible to minimize the use of chemically synthesized insecticidal active compounds.

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Abstract

[Problem] To provide a system having an excellent attracting effect on harmful arthropods. [Solution] A combination for controlling the behavior of harmful arthropods, comprising at least one compound selected from the group consisting of compounds that are attractive to the harmful arthropods, and at least one natural enemy selected from the group consisting of the natural enemies of the harmful arthropods, wherein the compound and the natural enemy are used in a mode where the harmful arthropods are synergetically attracted.

Description

有害節足動物誘引化合物と天敵の組み合わせ物Combination of harmful arthropod-attracting compounds and natural enemies
 本発明は、有害節足動物を対象に、有害節足動物を誘引するための誘引化合物、および有害節足動物に対する天敵を相乗効果的に併用することで、有害節足動物の被害から農作物を保護する防除方法および予察方法、或はこれらの方法において使用するための組み合わせ物に関する。また、かような誘引化合物および天敵を介して有害節足動物個体群の行動を制御する方法、特に施設栽培ハウス内および露地における、有害節足動物による植物の被害を防除および予察する方法、或はこれらの方法で使用するための組み合わせ物に関する。 The present invention synergistically combines an attracting compound for attracting harmful arthropods, and natural enemies against harmful arthropods in harmful arthropods, thereby preventing crops from harmful arthropod damage. The present invention relates to protective control methods and predictive methods to protect, or combinations for use in these methods. Also, a method for controlling the behavior of harmful arthropod populations via such attracting compounds and natural enemies, especially for controlling and predicting the damage of plants by harmful arthropods in institutional greenhouses and in open fields, or Relates to a combination for use in these methods.
 有害節足動物による栽培作物への被害は、世界的問題となっている。有害節足動物の中にはウイルスを媒介するものも存在し、ウイルス病を引き起こすことで作物に対してより深刻な被害を与えている。従来から、農作物に発生し被害をもたらす有害節足動物の防除は、多くの場合化学農薬により行われている。しかし、蕾など閉鎖された箇所に生息する微小な有害節足動物の場合、低密度での検出や化学農薬による防除は困難である。さらに、化学農薬に対する抵抗性の出現により防除効果が低下することや、化学農薬の使用による環境への影響が問題視されている。したがって、栽培作物及び植物に対する被害を低減するために有害節足動物個体群を検出し、有害節足動物個体群密度を抑制する方法が求められている。 Damage to cultivated crops by harmful arthropods is a global problem. Some harmful arthropods also carry viruses, causing more serious damage to crops by causing viral diseases. Conventionally, control of harmful arthropods that occur and cause damage to agricultural crops is often carried out with chemical pesticides. However, in the case of minute harmful arthropods that inhabit closed places such as cormorants, it is difficult to detect at low density and control with chemical pesticides. Furthermore, the control effect is lowered due to the emergence of resistance to chemical pesticides, and the environmental impact of the use of chemical pesticides is regarded as a problem. Therefore, there is a need for a method for detecting harmful arthropod populations and reducing the density of harmful arthropod populations in order to reduce damage to cultivated crops and plants.
 有害節足動物は寄主植物を利用するために、植物が生産し、放出する揮発性物質を用いている。したがって、植物由来の化合物のいくつかは、有害節足動物の誘引物質として作用し、誘引物質を添加した適切なトラップを使用して有害節足動物を誘引することが可能である(特許文献1、特許文献2、非特許文献1、非特許文献2)。 In order to use host plants, harmful arthropods use volatile substances produced and released by plants. Accordingly, some of the plant-derived compounds act as attractants for harmful arthropods, and it is possible to attract harmful arthropods using an appropriate trap to which attractants are added (Patent Document 1). Patent Document 2, Non-Patent Document 1, Non-Patent Document 2).
 ある種の生物が生産した物質が、生産した種には不利であるが、受容した他の種の生物に対して有益な効果を及ぼす物質としてのカイロモンは公知である。例えば、植物が生産し、受容した植食者が誘引される物質もカイロモンであり、これらの物質は植物由来のカイロモンとされる。誘引化合物として見出された物質の多くが、植物由来のカイロモンとして知られる芳香化合物である。多くの植物由来のカイロモンが各種有害節足動物の誘引物質として報告されてきた。例えば、ドウガネブイブイ(Anomala cuprea)のオス、メス成虫を誘引するカイロモンとして、青葉アルコールおよび、そのエステル類、メチルイソオイゲノールおよび、その類縁化合物が明らかにされており、(特許文献1、特許文献2)、これらを誘引剤として利用したトラップがすでに発生調査に利用されている。また、花の芳香であるベンジルアセテートとその誘導体6成分が、スギノアカネトラカミキリ(Cryptomeria twing)を誘引することが明らかにされており(特許文献3、特許文献4、特許文献5)、これらを誘引剤として利用したトラップもすでに発生調査に利用されている。 A substance produced by a certain organism is disadvantageous to the produced species, but kairomones are known as substances that have a beneficial effect on other species of organisms that have been received. For example, a substance produced by a plant and attracted by an accepted herbivore is a kairomone, and these substances are plant-derived kairomones. Many of the substances found as attracting compounds are aromatic compounds known as plant-derived kairomones. Many plant-derived kairomones have been reported as attractants of various harmful arthropods. For example, green leaf alcohol and its esters, methyl isoeugenol and its related compounds have been clarified as kairomones that attract male and female adults of Anomala cuprea (Patent Document 1, Patent Document 2). Traps using these as attractants have already been used for occurrence investigations. In addition, it has been clarified that benzyl acetate, which is the fragrance of flowers, and its six components induce Cyptomeria winging (Patent Document 3, Patent Document 4, Patent Document 5). Traps used as attractants have already been used for investigations.
 植物由来のカイロモンが、アザミウマ類の誘引物質として報告されてきた。例えば、p-アニスアルデヒド、ニコチン酸エチル、ベンズアルデヒド、o-アニスアルデヒド、β-ファルネセン、オイゲノール、3-フェニルプロピルアルデヒド、モノテルペン類(ゲラニオール、リナロール、ネロール、シトロネロール、ユーカリプトール)、モノテルペンエステル類(吉草酸イソボルニル、ピバリン酸イソボルニル、吉草酸ラバンジュリル)が、ミカンキイロアザミウマ(Frankliniella occidentalis)の誘引物質として報告されているが、その誘引性の程度は異なる(非特許文献3、非特許文献4、非特許文献5、非特許文献6、特許文献6、特許文献7、特許文献8)。 Plant-derived kairomones have been reported as attractants for thrips. For example, p-anisaldehyde, ethyl nicotinate, benzaldehyde, o-anisaldehyde, β-farnesene, eugenol, 3-phenylpropylaldehyde, monoterpenes (geraniol, linalool, nerol, citronellol, eucalyptol), monoterpene ester Class (isobornyl valerate, isobornyl pivalate, lavandulyl valerate) have been reported as attractants of Franklinella occidentalis, but the degree of attraction is different (Non-patent document 3, Non-patent document 4) Non-patent document 5, Non-patent document 6, Patent document 6, Patent document 7, Patent document 8).
 他にも、ニコチン酸エチルとベンズアルデヒドは、スリップス オブスクラタス(Thrips obscuratus)に対して誘引性を示し、アニスアルデヒドとベンズアルデヒドは、ネギアザミウマ(Thrips tabaci)に対して誘引性を示すことが報告されている。また、アントラニル酸メチル、アントラニル酸エチルは、ハナアザミウマ(Thrips hawaiiensis)、ビワハナアザミウマ(Thrips coloratus)を誘引するが、ミカンキイロアザミウマ、ネギアザミウマを誘引しないことが報告されている(非特許文献3、非特許文献7、非特許文献9)。
 カイロモン様活性を示すが植物由来でない化合物は植物由来のカイロモンの類縁体と称されることもある。例えば、ニコチン酸エチルとm-アミノ安息香酸メチルは、それぞれ植物由来の化合物であるニコチン酸メチルとo-アミノ安息香酸メチルの類縁体であり、植物由来の化合物ではないが、アザミウマ類の誘引物質として報告されている(非特許文献3、非特許文献5、非特許文献7、非特許文献8)。
In addition, ethyl nicotinate and benzaldehyde have been shown to be attractive to Trips obscuratus, and anisaldehyde and benzaldehyde have been shown to be attractive to Thrips tabaci. Yes. Further, it has been reported that methyl anthranilate and ethyl anthranilate induce Thrips hawaiiensis and Thrips coloratus, but do not attract citrus thrips and Negia thrips (Non-Patent Document 3). Non-patent document 7, Non-patent document 9).
A compound that exhibits kairomone-like activity but is not derived from a plant is sometimes referred to as an analog of a plant-derived kairomone. For example, ethyl nicotinate and methyl m-aminobenzoate are analogs of methyl nicotinate and methyl o-aminobenzoate, which are plant-derived compounds, respectively, and are not plant-derived compounds, but are attractants of thrips (Non Patent Literature 3, Non Patent Literature 5, Non Patent Literature 7, and Non Patent Literature 8).
 植物由来の芳香化合物以外にも、合成されたピリジン誘導体化合物(イソニコチン酸メチル、イソニコチン酸エチル、イソニコチン酸プロピル、イソニコチン酸イソプロピル、イソニコチン酸デシル、2-クロロ-イソニコチン酸エチル、ピリジン4-(1,3-ジオキソラン-2-イル)、ジ-イソプロピルイソニコチンアミド、4-ホルミルピリジン、メチル4-ピリジンケトン、エチル4-ピリジンケトン、プロピル4-ピリジンケトン)は、ネギアザミウマ、ミカンキイロアザミウマ、スリップス オブスクラタスに対して誘引性を示すことが報告されている(特許文献6、特許文献7)。 In addition to aromatic compounds derived from plants, synthesized pyridine derivative compounds (methyl isonicotiate, ethyl isonicotinate, propyl isonicotiate, isopropyl isonicotinate, decyl isonicotinate, ethyl 2-chloro-isonicotinate, Pyridine 4- (1,3-dioxolan-2-yl), di-isopropylisonicotinamide, 4-formylpyridine, methyl 4-pyridine ketone, ethyl 4-pyridine ketone, propyl 4-pyridine ketone), It has been reported that it attracts against citrus yellow thrips and Srips obscuratus (Patent Document 6, Patent Document 7).
 上記の物質は、アザミウマの種類によって異なる程度の誘引性を示す場合があり、種特異性を示すことが報告されている。 The above substances may show different levels of attractiveness depending on the type of thrips, and have been reported to show species specificity.
 一方で、生物防除法として天敵を栽培中の作物に処理することで、有害節足動物を防除し、作物の被害を防ぐ方法が行われてきた(特許文献9)。通常、天敵とは、自然界において作用する生物の個体数を制御する力、すなわち自然制御のうち、自然制御に関わる生物的要因内の捕食者や寄生者として機能する生物のことを指すが、本発明においても同様の概念を有するものとして使用する。さらに、昆虫に感染し、直接的あるいは間接的に致死させる病原菌やウイルスなども天敵であり、天敵微生物と称される。
 さらに、天敵の効果を高めるために、天敵の餌となる異種の昆虫が増加しやすい植物をバンカープラントとして栽培し、このバンカープラントに寄生した昆虫を餌とした天敵が増殖し、栽培作物に寄生した有害節足動物を捕食し作物の被害を回避する方法が行われている(特許文献10)。例えば、作物栽培施設内に麦を栽培してムギクビレアブラムシを寄生させ、さらにムギクビレアブラムシの天敵コレマンアブラバチを放飼して麦の上で増殖させておき、栽培施設内の作物に侵入寄生したワタアブラムシやモモアカアブラムシなどの重要有害節足動物を捕殺せしめ被害を未然に防ぐ方法が一般に使用されている。
On the other hand, a method for controlling harmful arthropods and preventing damage to the crops has been performed by treating natural enemies with cultivated crops as a biological control method (Patent Document 9). Usually, natural enemies refer to organisms that function as predators or parasites within the biological factors involved in natural control, the power that controls the number of living organisms acting in nature, that is, natural control. In the invention, it is used as having the same concept. Furthermore, pathogenic bacteria and viruses that infect insects and kill directly or indirectly are natural enemies and are called natural enemy microorganisms.
In addition, in order to enhance the effects of natural enemies, plants that tend to increase the number of different types of insects that feed on natural enemies are cultivated as bunker plants, and natural enemies that feed on insects parasitic on these bunker plants grow and parasitize cultivated crops. A method for avoiding damage to crops by predating harmful arthropods has been performed (Patent Document 10). For example, cultivate wheat in a crop cultivation facility to infest parasitoids, then release the natural enemy Koleman abrabachi of wheat beetle and propagate it on the wheat to infest the crops in the cultivation facility. Generally used is a method of killing important harmful arthropods such as cotton aphids and peach aphids to prevent damage.
 また、農作物を有害節足動物の被害から保護するために、農作物栽培施設又は圃場の内部あるいは外側に、有害節足動物の天敵昆虫が産卵し増殖する植物を栽培し、天敵昆虫を増加させることで有害節足動物を捕食せしめ、農作物の被害の発生を防ぐ方法もある(特許文献11)。例えば、有害節足動物であるアザミウマ類から農作物を保護するために、その天敵であるヒメハナカメムシ類の好適な産卵増殖植物であるカランコエを農作物栽培施設又は圃場の内部あるいは外側で栽培し、天敵のヒメハナカメムシ類を増殖させて生息密度を高め、有害節足動物のアザミウマ類の増加を有効に抑制する方法がある(特許文献11)。 In addition, to protect crops from harmful arthropod damage, cultivate plants that spawn and multiply harmful arthropod natural enemy insects inside or outside the crop cultivation facility or field, and increase natural enemy insects. There is also a method for preventing the occurrence of damage to crops by feeding on harmful arthropods (Patent Document 11). For example, in order to protect crops from thrips that are harmful arthropods, Kalanchoe, which is a suitable spawning plant of the common enemies, the domestic enemy, is cultivated inside or outside the crop cultivation facility or field, and the natural enemy There is a method of increasing the population density by increasing the number of the beetles, and effectively suppressing an increase in thrips of harmful arthropods (Patent Document 11).
 しかし、多くの天敵は、餌となる有害節足動物が生息しないと農作物栽培圃場内で生存することができない。また、天敵は圃場内の有害節足動物をすべて摂食すると、圃場外に移動する。さらに、餌や産卵場所があったとしても、圃場が温度や湿度が生息に適した環境条件でないと、天敵は圃場に定着せず、圃場外に移動する。このため、天敵を用いて、有害節足動物に対する増加抑制効果を持続的に発現させることは、天敵を利用した有害節足動物防除方法において大きな問題であった(特許文献12)。 However, many natural enemies cannot survive in the crop cultivation field unless harmful arthropods serving as prey live. Natural enemies move out of the field when all harmful arthropods in the field are eaten. Furthermore, even if there is a place for food and spawning, natural enemies will not settle in the field and move out of the field unless the field is in an environmental condition where the temperature and humidity are suitable for living. For this reason, it has been a big problem in the harmful arthropod control method using a natural enemy that the increase inhibitory effect with respect to a harmful arthropod is continuously expressed using a natural enemy (patent document 12).
 他にも、キュウリ栽培においては、有害節足動物であるアザミウマ類の著名な天敵であるタイリクヒメハナカメムシは、キュウリ株での増殖数が少なく、栽培圃場内に放虫しても、放した成虫により一時的なアザミウマ類の抑制効果を発現するのみで、持続的な抑制効果を示さなかった。また、イチゴ栽培では、有害節足動物カンザワハダニおよびナミハダニを防除するために、天敵であるチリカブリダニやミヤコカブリダニを放飼しているが、餌となる有害節足動物のダニ類をすべて摂食した場合、あるいは栽培圃場内が低湿度(63 % 以下)になった場合、天敵であるカブリダニ類が圃場から逃避するという問題があった(特許文献12)。 In addition, in cucumber cultivation, a common natural enemy of thrips, which are harmful arthropods, is a small number of cucumber strains that grow in cucumber strains and are released even if they are released in the cultivation field. Adults only exhibited a temporary thrips suppression effect, but did not show a sustained suppression effect. Moreover, in strawberry cultivation, in order to control the harmful arthropods Kanzawa spider mite and nymph spider mite, natural enemies dust mite and Miyako spider mite are released, but when all the harmful arthropod mites that feed are eaten Alternatively, when the humidity in the cultivation field is low (63% by weight or less), there is a problem that red mites that are natural enemies escape from the field (Patent Document 12).
 その他にも、ダニ類の発生が多いカンキツ類、ブドウ、ナシおよびリンゴ栽培では、化学農薬の殺ダニ剤に対して薬剤抵抗性が発達したことで化学農薬の効果が低下し、化学農薬のみでは有害節足動物を防除できない事態も頻発している。この解決策として、上記と同様に、有害節足動物の天敵昆虫を放して有害節足動物を防除しようとする試みも行われたが、天敵昆虫を放した直後の一時的な有害節足動物の密度抑制効果は認められるものの、多くの場合、有害節足動物防除効果に持続性を示さなかった(特許文献12)。 In addition, in citrus, grape, pear and apple cultivation with high occurrence of ticks, the resistance of chemical pesticides is reduced due to the development of drug resistance against chemical pesticides, and harmful only with chemical pesticides. There are also frequent situations in which arthropods cannot be controlled. As a solution to this problem, similar to the above, an attempt was made to release harmful arthropod natural enemy insects to control harmful arthropods, but temporary harmful arthropods immediately after releasing natural enemy insects. However, in many cases, the arthropod pest control effect was not sustained (Patent Document 12).
 ヒメハナカメムシ(Orius属)は、難防除有害節足動物であるアザミウマ類などの優れた天敵として知られており、かかる有害節足動物の防除方法として、ナミヒメハナカメムシ、タイリクヒメハナカメムシ等の利用が試みられている(特許文献13)。しかし、天敵を安定して供給するため、室内で大量飼育を行ったところ、近親交配を重ねたことによる近交弱勢が起こり、増殖率が低下するという新たな問題が起こっている。 Himehanakamushi (Orius genus) is known as an excellent natural enemy such as thrips, which are difficult-to-control harmful arthropods. As a method for controlling such harmful arthropods, Namihimehanamemushi, Tairikhimemekamemushi, etc. Has been tried (Patent Document 13). However, in order to stably supply natural enemies, large-scale breeding indoors has resulted in a new problem that inbreeding weakness due to repeated inbreeding has occurred and the growth rate has decreased.
 その上、捕食性ダニ類およびカメムシ類は、その捕食対象種数が必ずしも多くない(狭食性)ため、それらを利用した天敵によって防除可能な有害節足動物の種類が少数に限られるという欠点がある。また、この狭食性は、寄生性天敵、天敵微生物においても同様である。そのため、これら天敵を用いても、その天敵が防除できない有害節足動物は増殖、加害を繰り返すことになる。しかも、天敵は一般に薬剤感受性が高く、前記残存有害節足動物の防除を目的とする化学合成殺虫剤の同時使用は多くの場合不可能である。したがって、従来の天敵は、化学合成殺虫剤で問題になっていた欠点は克服したものの、生産および使用コスト、並びに効果の面には未だに問題が残っている(特許文献14)。 In addition, predatory mites and stink bugs do not necessarily have a large number of predatory species (narrow phagocytic), so there is a drawback that the number of harmful arthropods that can be controlled by natural enemies using them is limited to a small number. is there. Moreover, this narrow food property is the same also in parasitic natural enemies and natural enemy microorganisms. Therefore, even if these natural enemies are used, harmful arthropods that cannot be controlled by the natural enemies will repeat proliferation and harm. Moreover, natural enemies are generally highly drug sensitive, and in many cases, simultaneous use of chemically synthesized insecticides for the purpose of controlling the remaining harmful arthropods is impossible. Therefore, although the conventional natural enemy overcomes the drawbacks that have been a problem with chemically synthesized insecticides, problems still remain in terms of production and use costs and effects (Patent Document 14).
 天敵を利用して有害節足動物を防除する場合、一般的に、有害節足動物の密度が低い発生初期に天敵を処理するという方法が行われる。これは有害節足動物の密度が高くなってからでは、天敵による被害抑制効果が現れにくいためである。しかし、発生初期に有害節足動物を農作物栽培圃場で検出するには熟練を要し、見落としてしまうことが多い。このため、有害節足動物発生密度が高くなってから、一度化学農薬を散布した上で天敵を放飼するという手法がとられることが多い。このように天敵を利用する場合においても、有害節足動物防除効果の持続性のみならず、天敵を放すタイミングの決定の困難性という問題の解決が同時に求められていた(特許文献12)。 When controlling harmful arthropods using natural enemies, a method is generally used in which natural enemies are treated at the early stage of occurrence when the density of harmful arthropods is low. This is because after the density of harmful arthropods is increased, the effect of suppressing damage by natural enemies is unlikely to appear. However, in order to detect harmful arthropods in the crop cultivation field at the early stage of development, skill is required and often overlooked. For this reason, after the harmful arthropod generation density becomes high, a method of releasing natural enemies after spraying chemical pesticides is often used. Even in the case of using natural enemies as described above, not only the durability of the harmful arthropod control effect but also the problem of difficulty in determining the timing for releasing natural enemies has been sought at the same time (Patent Document 12).
 上述したこれらの問題は、タイリクヒメハナカメムシ、スワルスキーカブリダニ、チリカブリダニ、ミヤコカブリダニなどの天敵昆虫ばかりでなく、その他の多くの天敵類でも同様に、解決が求められている課題である(特許文献12)。 These problems described above are problems that are demanded to be solved not only by natural enemy insects such as Timber-winged stink bugs, Swarsky spider mites, dust mites, and Miyako spider mites, but also by many other natural enemies (Patent Literature). 12).
 したがって、上記の理由により天敵を単独で使用しただけでは、有害節足動物に対して高い防除効果を示さない事例もみられ、有害節足動物(例えばアザミウマ類など)の防除手段として用いるには不十分な場合もあった。 Therefore, there are cases where the use of natural enemies alone for the above reasons does not show a high control effect against harmful arthropods, and it is not possible to use them as a means of controlling harmful arthropods (eg thrips). Sometimes it was enough.
 このように、有害節足動物の行動を様々な因子を用いて制御しようとする試みは、以前から行われてきた。しかし、従来の方法は、誘引化合物(刺激因子)か天敵(抑制因子)のどちらかが単独で用いるものがほとんどであり、防除手段としては不十分であった。 Thus, attempts to control the behavior of harmful arthropods using various factors have been made for some time. However, most conventional methods use either attracting compounds (stimulating factors) or natural enemies (suppressing factors) alone, and are insufficient as control means.
 近年、化学農薬散布による環境負荷の問題、および薬剤抵抗性を持つ有害節足動物の問題から、複数の有害節足動物防除法等を合理的に組み合わせ、経済的な視点から、有害節足動物の密度や作物被害の変動等を見極めながら防除の要否を判断する技術として、「I n t e g r a t e d  P e s t  M a n a g e m e n t」(I P M) が注目されている。当該技術では、環境への負荷の増大が懸念される化学合成殺虫剤の使用を必要最小限に抑制することにより、安全・良質な農産物を安定生産することを基本としており、従来の生産性を維持した上で、持続的な農業の確立を目指すことが目標である。 In recent years, due to the problem of environmental burden caused by chemical pesticide spraying and the problem of harmful arthropods with drug resistance, it is possible to rationally combine multiple harmful arthropod control methods, etc. As a technology to determine the necessity of pest control while observing fluctuations in crop density and crop damage, etc., “Int eng r a te d P s M a a n e eng ent” (IP M) is attracting attention. This technology is based on the stable production of safe and high-quality agricultural products by minimizing the use of chemically synthesized insecticides, which are feared to increase the burden on the environment. The goal is to maintain sustainable agriculture while maintaining it.
 しかしながら、上記技術は多様でかつ複雑な要素の上に成り立つ技術であるために、当該技術を熟知した専門家の指導無く当該技術が導入された場合には、単に生産現場に減収のリスクを負わせることになりかねない状況が存在していた。 However, since the above technology is based on various and complex elements, if the technology is introduced without the guidance of an expert who is well-versed in the technology, there is simply a risk of reduced sales at the production site. There was a situation that could lead to
 上記課題を解決するための手段として、害虫管理手法としての「プッシュ・プル法」が近年、注目されている。「プッシュ・プル法」は、ワタを加害するタバコガ類を防除するための、「おとり作物」としてトウモロコシやキマメをワタ畑に植栽すると共に、害虫が忌避するニームオイルを施用することによって、おとり作物が害虫を引き寄せ(プル)、忌避剤が害虫を遠ざけることから、こう名付けられた(非特許文献11)。害虫管理手法としての「プッシュ・プル法」は、定位・定着・摂食・産卵といった昆虫の行動を刺激する因子と抑制する因子とを組み合わせることにより、圃場における昆虫個体群の分布と密度を意図的に調節する方法として定義されている(非特許文献10、非特許文献11)。ここで、刺激因子とは、植物由来のカイロモン、アロモン、昆虫由来のフェロモンといった化学的因子や、視覚刺激のような物理的因子などの刺激効果を示す因子であり、抑制因子とは忌避剤などの忌避効果を示す因子である。すなわち、昆虫の行動を制御する「刺激因子」と「抑制因子」を組み合わせる手法はすべてプッシュ・プル法である。プッシュ・プル法は、刺激因子と抑制因子の相反する作用因子を同時に使用して昆虫個体群を制御する点において、これまでの手法とは一線を画するものである。プッシュ・プル法とは、昆虫の行動を制御するあらゆる因子を活用し、それらを最も適切なデザインで配置することにより、害虫個体群を低いレベルに維持する方法であり、広義の総合的害虫管理技術といえる。 As a means for solving the above problems, “push-pull method” as a pest management technique has been attracting attention in recent years. The “push-pull method” is a decoy by planting corn and pigeons in cotton fields as “decoy crops” to control tobacco moths that harm cotton, and by applying neem oil that pests avoid. It was named because crops attracted (pull) pests and repellents kept pests away (Non-Patent Document 11). The “push-pull method” as a pest management method is intended to control the distribution and density of insect populations in the field by combining factors that stimulate insect behavior such as localization, colonization, feeding, and spawning. Is defined as a method of automatically adjusting (Non-Patent Document 10, Non-Patent Document 11). Here, the stimulating factor is a factor showing a stimulating effect such as a chemical factor such as a plant-derived kairomone, an allomone, or an insect-derived pheromone, or a physical factor such as a visual stimulus, and a suppressor is a repellent or the like It is a factor that shows the repellent effect. In other words, all the methods that combine “stimulating factors” and “suppressing factors” that control insect behavior are push-pull methods. The push-pull method is different from the conventional methods in that the insect population is controlled by simultaneously using the acting factors of the stimulating factor and the inhibitory factor. The push-pull method is a method to maintain the pest population at a low level by utilizing all the factors that control insect behavior and arranging them in the most appropriate design, and comprehensive pest management in a broad sense It can be said that technology.
 しかしながら、刺激因子や抑制因子の併用が効果的に作用するかどうかは、刺激因子と抑制因子の相互作用や主作物の位置関係などに依存する。刺激因子の誘引性が不十分であれば、害虫は隣接する主作物に誘引され、被害をもたらす可能性がある。あるいは、抑制因子の忌避性が不十分であれば、害虫は主作物に侵入し、主作物の被害が拡大する可能性がある。このように、プッシュ・プル法による刺激因子や抑制因子を使用することは一定のリスクが伴うという課題がある。 However, whether or not a combination of a stimulating factor and an inhibitory factor works effectively depends on the interaction between the stimulating factor and the inhibitory factor and the position of the main crop. If the attractiveness of the stimulating factor is insufficient, the pest can be attracted to the adjacent main crop and cause damage. Alternatively, if the repellent nature of the suppressor is insufficient, the pests can invade the main crop and the damage to the main crop can be magnified. Thus, there is a problem that using a stimulation factor or a suppression factor by the push-pull method involves a certain risk.
特開平7-242506号公報JP 7-242506 A 特開平9-124409号公報JP-A-9-124409 特開平3-188005号公報Japanese Patent Laid-Open No. 3-188005 特開平6-234603号公報JP-A-6-234603 特開平9-194303号公報JP-A-9-194303 特開2011-264654号公報JP 2011-264654 A 国際公開第2005/046330号明細書International Publication No. 2005/046330 Specification 国際公開第03/055309号明細書International Publication No. 03/0555309 Specification 特開2003-79271号公報JP 2003-79271 A 特開2003-929629号公報JP 2003-929629 A 特開2006-109726号公報JP 2006-109726 A 特開2006-158348号公報JP 2006-158348 A 特開平11-253069 号公報Japanese Patent Laid-Open No. 11-253069 特開2002-47116号公報JP 2002-47116 A
 有害節足動物を制御するための従来技術である、例えば、誘引物質の単独使用、天敵の単独使用はもとより、複数の手段の組み合わせ使用には、上述した短所ないしは問題点が存在する。したがって、本発明は、かような短所ないしは問題点を克服する手段を提供することを目的とする。 The conventional techniques for controlling harmful arthropods, such as the single use of attractants and the single use of natural enemies, as well as the combined use of multiple means, have the above-mentioned disadvantages or problems. Accordingly, an object of the present invention is to provide means for overcoming such disadvantages or problems.
 このような背景の下、本発明者らは鋭意検討を行った結果、処置の必要とする同一空間内で特定の誘引化合物と特定の天敵を同時に組み合わせ、使用すると該空間内、例えば、施設栽培ハウス内および露地、での有害節足動物防除に際して、農家が受け入れ易く、かつ、化学合成殺虫剤の使用を必要最小限に抑えることによって安全・良質な農産物を安定生産させることが可能になるという、新規かつ、極めて重要な技術的事項を見出した。こうして、前記課題は、有害節足動物に対して誘引性を有する化合物よりなる群から選ばれる少なくとも1種の化合物と、該有害節足動物に対する天敵よりなる群から選ばれる少なくとも1種の天敵を含んでなる、有害節足動物を制御するための、または制御する方法で使用するための組み合わせ物であって、前記化合物と天敵が、処置を必要とする場所で前記有害節足動物を相乗的に誘引する態様で使用される、ことを特徴とする組み合わせ物の提供により解決される。さらに、前記課題は、同一空間内で誘引化合物と天敵を矛盾なく相乗効果的に同時に併用することで、先行技術文献に記載された誘引化合物を単独で用いた場合と比較して、より強力に有害節足動物を誘引でき、そのため、より効果的に有害節足動物個体群の行動が制御でき、特に有害節足動物による作物の被害を防除および/または予察することのできる方法またはデバイスを提供することにより解決される。 Under such a background, as a result of intensive studies, the present inventors have simultaneously combined and used a specific attracting compound and a specific natural enemy in the same space in need of treatment, for example, in the space, for example, facility cultivation When controlling harmful arthropods in the house and on the open ground, it is easy for farmers to accept, and it is possible to stably produce safe and high-quality agricultural products by minimizing the use of chemically synthesized insecticides. I found a new and extremely important technical matter. Thus, the object is to provide at least one compound selected from the group consisting of compounds attracting harmful arthropods and at least one natural enemy selected from the group consisting of natural enemies against the harmful arthropods. A combination for use in a method of controlling or controlling a harmful arthropod, wherein the compound and natural enemy synergize the harmful arthropod in a location in need of treatment. It is solved by providing a combination characterized in that it is used in an attracting manner. Furthermore, the above-mentioned problem is more powerful compared with the case where the attracting compound described in the prior art document is used alone by simultaneously using the attracting compound and the natural enemy simultaneously and synergistically without contradiction in the same space. Providing a method or device that can attract harmful arthropods and thus more effectively control the behavior of harmful arthropod populations, and in particular to control and / or predict crop damage by harmful arthropods It is solved by doing.
 作物上に生息する特定の有害節足動物が特定の天敵の攻撃、捕食行動、寄生行動を受けた場合、天敵を回避するため生息していた作物上から移動する。そのとき、作物が栽培されている栽培ハウスまたは露地を特定の誘引物質で処理すると誘引物質が有害節足動物を誘引し、誘引物質を単独で使用する場合よりも誘引効果が上昇する。したがって、上述のとおり、同一空間内における本発明の誘引化合物および天敵の同時併用は、優れた有害節足動物誘引効果を有するので、効率よく有害節足動物を誘引、捕獲することができ、作物に被害を与える有害節足動物の密度を効率よく抑制することが可能となる。このような作用、効果を発揮すべく誘引化合物および天敵を同時に使用して有害節足動物を防除することにより、各種植物を効果的に保護することが可能となる。 When a specific harmful arthropod that inhabits a crop is attacked, preyed, or parasitized by a specific natural enemy, it moves from the crop that lived to avoid the natural enemy. At that time, if the cultivation house or the open field where the crop is cultivated is treated with a specific attractant, the attractant attracts harmful arthropods, and the attracting effect is higher than when the attractant is used alone. Therefore, as described above, the simultaneous use of the attracting compound of the present invention and the natural enemy in the same space has an excellent effect of attracting harmful arthropods, so that it can efficiently attract and capture harmful arthropods, It is possible to efficiently control the density of harmful arthropods that cause damage. It is possible to effectively protect various plants by controlling the harmful arthropods by simultaneously using the attracting compound and the natural enemy to exert such actions and effects.
発明の詳細な記述Detailed description of the invention
 本発明について説明する用語は、別に定義しないときは、当該技術分野で常用されている意味、内容を有するものと理解されている。本発明の一の態様は、有害節足動物に対して誘引性を有する化合物よりなる群から選ばれる少なくとも1種の化合物と、該有害節足動物に対する天敵よりなる群から選ばれる少なくとも1種の天敵を含んでなる、有害節足動物を制御するためのまたは制御する方法で使用するための組み合わせであって、前記化合物と天敵が、処置の必要とする場所で前記有害節足動物を相乗的に誘引する態様で使用される、ことを特徴とする組み合わせ物である。 Unless otherwise defined, terms used to describe the present invention are understood to have meanings and contents commonly used in the technical field. One aspect of the present invention is at least one compound selected from the group consisting of compounds having attractiveness to harmful arthropods and at least one compound selected from the group consisting of natural enemies against the harmful arthropods. A combination for use in a method of controlling or controlling a harmful arthropod comprising a natural enemy, wherein the compound and the natural enemy synergize the harmful arthropod in a location where treatment is required. It is a combination characterized by being used in an attracting manner.
 処置の必要とする場所は、本発明の目的に沿って所期の効果が達成できる場所であれば特に限定されるものでないが、例えば、施設栽培ハウス内および露地等の同一空間であることができる。このように、特定の誘引物質と特定の天敵を同一空間内で同時に併用することにより従来の技術を凌駕した誘引効果が達成されるので、安全・良質な農産物を安定生産させるために極めて有用である有害節足動物防除方法を汎用技術化することが可能となる。さらに本発明は、農家が受け入れ易く、かつ、化学合成殺虫剤の使用を必要最小限に抑えることができる。 The place where treatment is required is not particularly limited as long as the desired effect can be achieved in accordance with the purpose of the present invention, but for example, it may be the same space such as in a facility cultivation house and an open field. it can. In this way, a specific attracting substance and a specific natural enemy are used in the same space at the same time to achieve an attraction effect that surpasses conventional techniques, so it is extremely useful for stable production of safe and high-quality agricultural products. It becomes possible to generalize a certain arthropod control method. Furthermore, the present invention is easy for farmers to accept and can minimize the use of chemically synthesized insecticides.
 本発明は、個別には、当該技術分野で既に提案された、有害節足動物に対して誘引性を有する化合物を使用する該有害節足動物の誘引方法を包含する方法と、有害節足動物に対する天敵を使用する該有害節足動物の忌避方法を包含する方法との組み合わせ使用に関する。このような組み合わせ使用は、有害節足動物の処置の必要な場所、例えば、施設栽培ハウスおよび露地での有害節足動物防除方法、施設栽培ハウスおよび露地における同一の空間で実施される。実施の時期は、本発明の目的に沿った所期の効果を達成できる限り、限定されるものでないが、いずれか一方の方法を先に、その他の方法を後に、相互にタイミングをずらして実施するか、または両者を同時に実施してもよい。こうすることにより、有害節足動物は、前記化合物により一定の場所に誘引され、一方で、前記天敵によりその場に留まることを忌避せしめることにより前記一定の場所に集める、新たな手段の組み合わせによる、所謂、プッシュ・プル法に該当し、I P M と呼ばれている総合防除技術のうちの一部分を構成する技術が提供される。そのため、それらを個別に用いた時の効果を加算した効果を凌駕する効果が得られる。したがって、化学合成殺虫剤の施用量を削減可能にするので施設栽培ハウスおよび露地での有害節足動物防除方法において用いられる化学合成殺虫剤に対する有害節足動物における薬剤抵抗性の発達を抑制することも可能になる。 The present invention separately relates to a method including a method for attracting harmful arthropods using a compound that has been attracted to harmful arthropods already proposed in the art, and a harmful arthropod, The present invention relates to a combined use with a method including a method for repelling the harmful arthropod using natural enemies. Such combined use is carried out in the same space in places where treatment of harmful arthropods is required, for example, methods for controlling harmful arthropods in institutional cultivation houses and outdoor areas, institutional cultivation houses and outdoor areas. The timing of the implementation is not limited as long as the desired effect according to the object of the present invention can be achieved, but one of the methods is performed first, the other method is performed later, and the timing is mutually shifted. Or both may be performed simultaneously. In this way, the harmful arthropods are attracted to a certain place by the compound, while at the same time, by a combination of new means to collect in the certain place by avoiding the natural enemies from staying there. A technology that constitutes a part of the comprehensive control technology corresponding to the so-called push-pull method and called I P M is provided. Therefore, the effect which surpasses the effect which added the effect when using them individually is acquired. Therefore, the development of drug resistance in harmful arthropods against chemically synthesized pesticides used in controlled cultivation arthropod pesticides in house-cultivated houses and open fields can be suppressed as it enables the application rate of chemically synthesized insecticides to be reduced Is also possible.
 本発明の方法または組み合わせ物において処置の対象となる有害節足動物としては、限定されるものでないが、例えば、アザミウマ類、コナジラミ類、アブラムシ類、ハモグリバエ類、カメムシ目類、チョウ目、並びに、ハダニ類、等を挙げることができる。処置は、広範な概念として使用しており、概括的には、当該動物の誘引反応の促進が達成できるように、当該動物が取り扱われることを意味する。 Examples of harmful arthropods to be treated in the method or combination of the present invention include, but are not limited to, for example, thrips, whiteflies, aphids, leafworms, stink bugs, butterflies, and Spider mites can be mentioned. Treatment has been used as a broad concept and generally means that the animal is handled so that an enhanced attraction response of the animal can be achieved.
 本発明において、「相乗作用」または「相乗的に作用する」とは、特定の有害節足動物個体群に対して誘引性を有する特定の化合物より選ばれる少なくとも1種の化合物と特定の有害節足動物個体群に対する1種以上の特定の天敵とを同時に組み合わせた場合の誘引効果が、当該組み合わせの期待される効果(所謂、相加的な効果)より有意に高くなる作用を意味する。一般に、与えられた1種類の誘引成分化合物と1種類の天敵を併用して処理した際に期待される誘引相乗効果は下記の式1のコルビーの計算式により求められる。
「式1」;E=X+Y-(X*Y)/100
X:有効成分化合物Aを処理したときの誘引率
Y:天敵Bを処理したときの誘引率
E:有効成分化合物Aおよび天敵Bを併用して処理したときに期待される誘引率(誘引率期待値)
誘引物質と天敵を組み合わせた場合の誘引率が、誘引率期待値以上であった場合に、誘引相乗効果を示すとみなされる(S.R.Colby,(1967)Weeds,15,20-22参照)。このような作用は、室内生物検定または、圃場試験を実施することにより、容易に確認することができる。
In the present invention, “synergistic action” or “acting synergistically” means at least one compound selected from a specific compound having attraction to a specific harmful arthropod population and a specific harmful node. This means that the attraction effect when combining one or more specific natural enemies with a paw animal population at the same time is significantly higher than the expected effect (so-called additive effect) of the combination. In general, the attraction synergistic effect that is expected when one kind of attracting component compound and one natural enemy are used in combination is determined by the following Colby calculation formula.
“Formula 1”; E = X + Y− (X * Y) / 100
X: Attraction rate when the active ingredient compound A is treated Y: Attraction rate when the natural enemy B is treated E: Attraction rate expected when the active ingredient compound A and the natural enemy B are used in combination (expectation rate expectation) value)
When the attracting rate when the attracting substance and natural enemy are combined is greater than or equal to the expected attracting rate, the attracting synergistic effect is considered (see SR Colby, (1967) Weeds, 15, 20-22). ). Such an effect can be easily confirmed by performing a laboratory bioassay or a field test.
 したがって、本発明にいう、「有害節足動物を相乗的に誘引する態様」とは、有害節足動物に対して誘引性を有する化合物と天敵とが、それらの処置を必要とする同一の空間に置かれたときに、上記の等式に計算した場合に、誘引率期待値を超える値となるように、それらの組み合わせが選択されている状態を意味する。こうして、上記のような誘引性の相乗作用を確認できるものであれば、有害節足動物に対して誘引性を有する化合物よりなる群から選ばれる1種以上の化合物を誘引物質として用いる場合、天敵との組み合わせはいかなるものであっても本発明の範囲内に入る。 Therefore, the “mode of synergistically attracting harmful arthropods” referred to in the present invention is the same space in which the compound having the attractiveness to harmful arthropods and the natural enemy need their treatment. Means that the combination is selected so that the value exceeds the expected value of the attraction rate when calculated in the above equation. Thus, if one or more compounds selected from the group consisting of compounds having attraction to harmful arthropods can be used as the attracting substance as long as the above-mentioned synergistic action of attraction can be confirmed, Any combination of and falls within the scope of the present invention.
 本発明で用いられる誘引物質として用いる化合物(以下、単に誘引物質をいう場合あり)は、対象の有害節足動物に誘引性を示す物質であればよい。限定されるものでないが、例えば、動物由来のカイロモンまたは植物由来のカイロモンである、p-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、ゲラニオール、リナロール、ネロール、シトロネロール、o-アニスアルデヒド、β-ファルネセン、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、オイゲノール、3-フェニルプロピルアルデヒド、シンナムアルデヒド、ユーカリプトール、スクアレン、α-ヘキシルシンナムアルデヒド、その他誘引物質であるネリル-S-メチルブタノエート、ラバンズリルアセテート、イソニコチン酸エチル、イソニコチン酸メチル、イソニコチン酸プロピル、イソニコチン酸イソプロピル、2-クロロ-イソニコチン酸エチル、ピリジン4-(1,3-ジオキソラン-2-イル) 、ジ-イソプロピルイソニコチンアミド、4-ホルミルピリジン、メチル4-ピリジルケトン、エチル4-ピリジルケトン、プロピル4-ピリジルケトン、青葉アルコール、青葉アルデヒド、青葉アセテート、α-ピネン、β-ピネン、アネトール、メチルオイゲノール、メチルフェニルアセテート、フェニルエチルプロピオネート、Z5-テトラデセン酸メチル、(R・Z)-(-)-5-(1-デセニル)オキサシクロペンタン-2-オン、シス-7-テトラデセン-2-オン、トランス-7-テトラデセン-2-オン、2,6-ジメチルオクチルホルメート、2,6-ジメチルオクタン-1-オール、2,3-ジヒドロ-2,3,5-トリメチル-6-(1-メチル-2-ブテニル)-4H-ピラン-4-オン、4,6-ジメチル-7-ヒドロキシノナン-3-オン、2,6-ジメチルオクタン-1-オール、(3Z,6Z,9S,10R)-9,10-エポキシ-3,6-ヘニコサジエン、(3Z,6Z,9S,10R)-9,10-エポキシ-1,3,6-ヘニコサトリエン、Z9,Z12,Z15-オクタデカトリエナール、(E)-9,11-ドデカジエニルブチラート、(E)-9,11-ドデカジエニルヘキサノアート、(Z)-3-ドデセニル(E)-2-ブテノアート、ミルセン、カンフェン、3-カレン、Z3-ドデセニルE2-ブテノアート、n-ヘキシル n-ヘキセノアート、n-オクチル n-ブチレート、E-2-ヘキセニル n-ヘキセノアート、3-メチル-6-イソプロペニル-9-デセニールアセテート、3Z-3-メチル-6-イソプロペニル-3,9-デカジエニルアセテート、4-(p-アセトキシフェニル)-2-ブタノン、4-(p-プロピオニルオキシフェニル)-2-ブタノン、4-(p-ヒドロキシフェニル)-2-ブタノン、4-(p-ブチリルオキシフェニル)-2-ブタノン、4-(p-イソバレリルオキシフェニル)-2-ブタノン、4-(p-メトキシフェニル)-2-ブタノン、酢酸フェネチル、プロピオン酸フェネチル、アクリル酸フェネチル、酢酸フェネチル、シクロプロパンカルボン酸フェネチル、ヒドロ桂皮酸エチル、4-フェニル酪酸メチル、酢酸-2-シクロヘキシルエチル、プロピオン酸-2-シクロヘキシルエチル、クロトン酸ヘプチル、2-ペンテン酸ヘキシル、2-ペンテン酸ヘプチル、チグリン酸ヘキシル、チグリン酸ヘプシル、2-ヘキセン酸ヘキシル、2-ヘプチン酸エチル、ペンタン酸シクロヘキシル、シクロヘキサン酢酸プロピル、(Z)-5-(1-デセニル)ジヒドロ-2(3H)-フラノン、Z5-テトラデセン酸メチル、Z,E-ファルネゾール、Z-ネロリドール、ステゴビノン、ステゴビオール、イソロイシンメチルなどが挙げられる。このうち、p-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、ゲラニオール、リナロール、ネロール、シトロネロール、o-アニスアルデヒド、β-ファルネセン、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、オイゲノール、3-フェニルプロピルアルデヒド、シンナムアルデヒド、ユーカリプトール、スクアレン、α-ヘキシルシンナムアルデヒドを含むことが好ましく、p-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、ゲラニオール、リナロール、ネロール、シトロネロール、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、α-ヘキシルシンナムアルデヒドを含むことがより好ましく、p-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、α-ヘキシルシンナムアルデヒドを含むことがさらに好ましい。 The compound used as an attracting substance used in the present invention (hereinafter sometimes simply referred to as an attracting substance) may be any substance that is attractive to the target arthropod. Without limitation, for example, p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, β-farnesene, anthranyl, which are animal-derived kairomones or plant-derived kairomones Methyl acid, methyl benzoate, o-aminoacetophenone, o-anisidine, methyl m-aminobenzoate, methyl o-toluate, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene, α-hexylcinna Mualdehyde, other attractants such as neryl-S-methylbutanoate, lavandulyl acetate, ethyl isonicotinate, methyl isonicotinate, propyl isonicotiate, isopropyl isonicotiate, 2- Ethyl chloro-isonicotinate, pyridine 4- (1,3-dioxolan-2-yl), di-isopropylisonicotinamide, 4-formylpyridine, methyl 4-pyridyl ketone, ethyl 4-pyridyl ketone, propyl 4-pyridyl Ketone, green leaf alcohol, green leaf aldehyde, green leaf acetate, α-pinene, β-pinene, anethole, methyl eugenol, methyl phenyl acetate, phenyl ethyl propionate, methyl Z5-tetradecenoate, (R · Z)-(-)- 5- (1-decenyl) oxacyclopentan-2-one, cis-7-tetradecen-2-one, trans-7-tetradecen-2-one, 2,6-dimethyloctylformate, 2,6-dimethyloctane -1-ol, 2,3-dihydro-2,3,5-trimethyl-6- ( -Methyl-2-butenyl) -4H-pyran-4-one, 4,6-dimethyl-7-hydroxynonan-3-one, 2,6-dimethyloctane-1-ol, (3Z, 6Z, 9S, 10R ) -9,10-epoxy-3,6-hexicosadien, (3Z, 6Z, 9S, 10R) -9,10-epoxy-1,3,6-henicosatriene, Z9, Z12, Z15-octadecatrienal, ( E) -9,11-dodecadienyl butyrate, (E) -9,11-dodecadienyl hexanoate, (Z) -3-dodecenyl (E) -2-butenoate, myrcene, camphene, 3-carene , Z3-dodecenyl E2-butenoate, n-hexyl n-hexenoate, n-octyl n-butyrate, E-2-hexenyl n-hexenoate, 3-methyl- -Isopropenyl-9-decenyl acetate, 3Z-3-methyl-6-isopropenyl-3,9-decadienyl acetate, 4- (p-acetoxyphenyl) -2-butanone, 4- (p-propionyl) Oxyphenyl) -2-butanone, 4- (p-hydroxyphenyl) -2-butanone, 4- (p-butyryloxyphenyl) -2-butanone, 4- (p-isovaleryloxyphenyl) -2- Butanone, 4- (p-methoxyphenyl) -2-butanone, phenethyl acetate, phenethyl propionate, phenethyl acrylate, phenethyl acetate, phenethyl cyclopropanecarboxylate, ethyl hydrocinnamate, methyl 4-phenylbutyrate, acetic acid-2- Cyclohexylethyl, 2-cyclohexylethyl propionate, heptyl crotonic acid, 2-pen Hexyl tenate, heptyl 2-pentenoate, hexyl tiglate, hepsyl tiglate, hexyl 2-hexenoate, ethyl 2-heptinate, cyclohexyl pentanoate, propyl cyclohexaneacetate, (Z) -5- (1-decenyl) dihydro -2 (3H) -furanone, methyl Z5-tetradecenoate, Z, E-farnesol, Z-nerolidol, stegovinone, stegoviol, isoleucine methyl and the like. Of these, p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, β-farnesene, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-anisidine, m- It preferably contains methyl aminobenzoate, methyl o-toluate, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene, α-hexylcinnamaldehyde, p-anisaldehyde, benzaldehyde, ethyl nicotinate, Geraniol, linalool, nerol, citronellol, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-anisidine, methyl m-aminobenzoate, o-toluic acid More preferably, it includes chill, α-hexylcinnamaldehyde, p-anisaldehyde, benzaldehyde, ethyl nicotinate, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-anisidine, methyl m-aminobenzoate, o More preferably, it comprises methyl toluate, α-hexylcinnamaldehyde.
 上記誘引物質は1種類だけを用いることに限定されず、2種以上の化合物を組み合わせて用いてもよく、その比率はいかなるものであってもよい。しかし、例えば、2種の化合物の組み合わせにあっては限定されるものではないが、2種類の化合物の組み合わせの重量比率が0.0999~99.9001:99.9001~0.0999、好ましくは0.1996~99.8004:99.8004~0.1996、より好ましくは0.99~90.01:90.01~0.99であることができる。 The above attractant is not limited to using only one kind, and two or more kinds of compounds may be used in combination, and the ratio thereof may be any. However, for example, the combination of two kinds of compounds is not limited, but the weight ratio of the combination of two kinds of compounds is 0.0999 to 99.9001: 99.9001 to 0.0999, preferably It can be 0.1996 to 99.8004: 99.8044 to 0.1996, more preferably 0.99 to 90.01: 90.01 to 0.99.
 上記誘引物質は3種以上の化合物を組み合わせて用いてもよく、その比率はいかなるものであってもよい。しかし、例えば、3種の化合物の組み合わせにあっては限定されるものではないが、3種類の化合物の組み合わせの重量比率が0.0998~99.8004:99.8004~0.0998:0.0998~99.8004、好ましくは0.1992~99.6016:99.6016~0.1992:0.1992~99.6016、より好ましくは0.98039~98.03922:98.03922~0.98039:0.98039~98.03922であることができる。 The attractant may be used in combination of three or more compounds, and the ratio thereof may be any. However, for example, the combination of the three compounds is not limited, but the weight ratio of the combination of the three compounds is 0.0998 to 99.8004: 99.8044 to 0.0998: 0.0.0. 0998 to 99.8004, preferably 0.1992 to 99.6016: 99.616 to 0.1992: 0.1992 to 99.6016, more preferably 0.98039 to 98.03922: 98.03922 to 0.98039 : 0.98039 to 98.03922.
 本発明に用いられる誘引物質はいずれも公知の化合物であり、これらの公知の化合物は市販のものをそのまま用いるか、市販の製剤から得るか、公知の方法により合成したものを用いることができる。本発明に用いられる誘引物質は、誘引性を有する化合物群から選ばれる1種類以上の化合物をそのまま、または適当な溶剤、例えば、ヘキサン、キシレン、アセトン、ジクロロメタンなどに溶かして溶液とするか、さらに、この溶液を適当な担体または保持体、例えば紙、布などに含浸させて製剤化して使用することも可能である。また、上記2種類以上の誘引活性物質を別々に製剤化して同一箇所において組み合わせて使用することも可能である。 The attracting substances used in the present invention are all known compounds, and as these known compounds, commercially available compounds can be used as they are, obtained from commercially available preparations, or synthesized by a known method. The attracting substance used in the present invention may be prepared by dissolving one or more compounds selected from the group of compounds having attraction as it is or by dissolving in an appropriate solvent such as hexane, xylene, acetone, dichloromethane, or the like. It is also possible to use this solution by impregnating it with a suitable carrier or holding body such as paper or cloth. It is also possible to formulate the two or more types of attracting active substances separately and use them in combination at the same location.
 本発明で用いる誘引物質は、粘着トラップ、コーントラップ、ファネルトラップ、水盤トラップ等の捕獲器に誘引源として用いることにより有害節足動物を効率的に捕殺することが可能である。本発明の誘引物質を誘引源として利用する捕獲器は、有害節足動物の発生場所、または飛来地域、すなわち室内、施設内、あるいは野外に設置して使用することも可能である。また捕獲器は着色して使用してもよい。 The attracting substance used in the present invention can efficiently kill harmful arthropods by using it as an attracting source in traps such as adhesive traps, corn traps, funnel traps, and basin traps. The trap using the attracting substance of the present invention as an attracting source can also be used by installing it in a place where harmful arthropods are generated or flying, that is, indoors, facilities, or outdoors. In addition, the trap may be used by coloring.
 本発明の誘引物質と天敵の組み合わせ物はまた、有害節足動物によって起こる植物への被害を予測し、または最小限に抑える方法、またはかような方法の補助用としての使用も可能である。 The combination of attractant and natural enemy of the present invention can also be used to predict or minimize damage to plants caused by harmful arthropods, or as an aid to such methods.
 本発明に用いられる天敵としては、例えば、捕食性天敵、寄生性天敵、天敵微生物などが挙げられる。このうち、前記天敵が捕食性天敵であるスワルスキーカブリダニ(Amblyseius swirskii)、チリカブリダニ(Phytoseiulus persimilis)、ミヤコカブリダニ(Neoseiulus californicus)、キイカブリダニ(Gynaeseius liturivorus)、ヘヤカブリダニ(Neoseiulus barkeri)、リモニカスカブリダニ(Amblydromalus limonicus)、ククメリスカブリダニ(Amblyseius cucumeris)、ニセラーゴカブリダニ(Amblyseius eharai)、コウスゲカブリダニ(Euseius sojaensis)、デジェネランスカブリダニ(Amblyseius degenerans)、ショクガタマバエ(Aphidoletes aphidimyza)、タイリクヒメハナカメムシ(Orius strigicollis)、ナミテントウ(Harmonia axyridis)、アリガタシマアザミウマ(Franklinothrips vespiformis)、アカメガシワクダアザミウマ(Haplothrips brevitubus)、ヒメカメノコテントウ(Propylea japonica)、タバコカスミカメ(Nesidiocoris tenuis)、クロヒョウタンカスミカメ(Pilophorus typicus)、ヤマトクサカゲロウ(Chrysoperla carnea)、オオメカメムシ(Geocoris varius)、寄生性天敵であるオンシツツヤコバチ(Encarsia formosa)、イサエアヒメコバチ(Diglyphus isaea)、ハモグリコマユバチ(Dacnusa sibirica)、コレマンアブラバチ(Aphidius colemani)、サバクツヤコバチ(Eretmocerus eremicus)、ハモグリミドリヒメコバチ(Neochrysocharis formosa)、チャバラアブラコバチ(Aphelinus asychis)、コナガサムライコマユバチ(Cotesia vestalis)、および天敵微生物であるパスツーリア ペネトランス(Pasteuria penetrans)、チャハマキ顆粒病ウイルス(Homona magnanimagranulovirus)、リンゴコカクモンハマキ顆粒病ウイルス(Adoxophyes orana granulovirus)、スタイナーネマカーポカプサエ(Steinernema carpocapsae)、スタイナーネマグラセライ(Steinernema glaseri)、モナクロスポリウムフィマトパガム(Monacrosporium phymatophagum)、ボーベリアブロンニアティ(Beauveria brongniartii)、ペキロマイセス フモソロセウス(Paecilomyces fumosoroseus)、ボベリアバシアーナ(Beauveria bassiana)、バーティシリウムレカニー(Verticillium lecanii)、ペキロマイセステヌイペス(Paecilomyces tenuipes)、ハスモンヨトウ核多角体病ウイルス(Spodoptera litura Nucleopolyhedrovirus)、アグロバクテリウムラジオバクター(Agrobacterium radiobactor)、非病原性エルビニアカロトボーラ(Erwinia carotovora)、バチルスズブチリス(Bacillus subtilis)、タラロマイセス フラバス(Talaromyces Flavus)、バチルスシンプレクス(Bacillus simplex)、トリコデルマアトロビリデ(Trichoderma Atroviride)、シュードモナスフルオレッセンス(Pseudomonas fluorescens)、コニオチリウムミニタンス(Coniothyrium minitans)、ズッキーニ黄斑モザイクウイルス(Zucchini yellow mosaic virus)、バチルスチューリンゲンシス(Bacillus thuringiensis)を含むことが好ましく、捕食性天敵であるスワルスキーカブリダニ(Amblyseius swirskii)、チリカブリダニ(Phytoseiulus persimilis)、ミヤコカブリダニ(Neoseiulus californicus)、キイカブリダニ(Gynaeseius liturivorus)、ヘヤカブリダニ(Neoseiulus barkeri)、リモニカスカブリダニ(Amblydromalus limonicus)、ククメリスカブリダニ(Amblyseius cucumeris)、ニセラーゴカブリダニ(Amblyseius eharai)、コウスゲカブリダニ(Euseius sojaensis)、デジェネランスカブリダニ(Amblyseius degenerans)、ショクガタマバエ(Aphidoletes aphidimyza)、タイリクヒメハナカメムシ(Orius strigicollis)、ナミテントウ(Harmonia axyridis)、アリガタシマアザミウマ(Franklinothrips vespiformis)、アカメガシワクダアザミウマ(Haplothrips brevitubus)、ヒメカメノコテントウ(Propylea japonica)、タバコカスミカメ(Nesidiocoris tenuis)、クロヒョウタンカスミカメ(Pilophorus typicus)、ヤマトクサカゲロウ(Chrysoperla carnea)、オオメカメムシ(Geocoris varius)、および寄生性天敵であるオンシツツヤコバチ(Encarsia formosa)、イサエアヒメコバチ(Diglyphus isaea)、ハモグリコマユバチ(Dacnusa sibirica)、コレマンアブラバチ(Aphidius colemani)、サバクツヤコバチ(Eretmocerus eremicus)、ハモグリミドリヒメコバチ(Neochrysocharis formosa)、チャバラアブラコバチ(Aphelinus asychis)、コナガサムライコマユバチ(Cotesia vestalis)、を含むことがより好ましく、捕食性天敵であるスワルスキーカブリダニ(Amblyseius swirskii)、チリカブリダニ(Phytoseiulus persimilis)、ミヤコカブリダニ(Neoseiulus californicus)、キイカブリダニ(Gynaeseius liturivorus)、ヘヤカブリダニ(Neoseiulus barkeri)、リモニカスカブリダニ(Amblydromalus limonicus)、ククメリスカブリダニ(Amblyseius cucumeris)、ニセラーゴカブリダニ(Amblyseius eharai)、コウスゲカブリダニ(Euseius sojaensis)、デジェネランスカブリダニ(Amblyseius degenerans)、ショクガタマバエ(Aphidoletes aphidimyza)、タイリクヒメハナカメムシ(Orius strigicollis)、ナミテントウ(Harmonia axyridis)、アリガタシマアザミウマ(Franklinothrips vespiformis)、アカメガシワクダアザミウマ(Haplothrips brevitubus)、ヒメカメノコテントウ(Propylea japonica)、タバコカスミカメ(Nesidiocoris tenuis)、クロヒョウタンカスミカメ(Pilophorus typicus)、ヤマトクサカゲロウ(Chrysoperla carnea)、オオメカメムシ(Geocoris varius)を含むことがさらに好ましい。 Examples of natural enemies used in the present invention include predatory natural enemies, parasitic natural enemies, natural enemy microorganisms, and the like. Among them, the natural enemies are predatory natural enemies, such as Swallowsky spider mites (Amblyseius swirskii), Chilean spider mites (Phytoseiulius persimilis), Mite spider mites (Neuseilus caulifornicus), Ipterus tuna (Gypsum spp.) (Amblydromalus limonicus), Kumeliska burid mite (Amblyseius cucumeris), Nicellago burdock mite (Amblyseius esharais), Kousegekaburidani (Euseius sojaensis), Degenerance burdock mite (Ausbius bulgari) nerans), food moth gall midge (Aphidoletes aphidimyza), Thailand Riku Orius (Orius strigicollis), Harmonia axyridis (Harmonia axyridis), Aliga Tashima thrips (Franklinothrips vespiformis), Mallotus japonicus Kedah thrips (Haplothrips brevitubus), Princess turtle saw ladybird (Propylea japonica), Tobacco turtle (Nesidiocoris tenuis), Black gourd turtle (Pilophorus typicus), Yamasokusakae (Chrysoperla carnea), Greater beetle (Geocoris varius), Parasitic natural enemy A certain honeybee (Encarsia formosa), Isaea himefachi (Diglyphus isafea), Dacnus siamusi (Dacnusa siberus), Aphidius cosmea Rose hornets (Aphelinus asychis), Japanese horned beetle (Cotesia vestalis), and Pasteuria penetrances that are natural enemy microorganisms, Chahamaki granulosis virus (Homona magnanimarangranus) Apple Coca summer fruit moth granules disease virus (Adoxophyes orana granulovirus), Sutainanema Capo capsule Sae (Steinernema carpocapsae), Sutainanema Gurase Lai (Steinernema glaseri), Mona Kurosupo potassium Fi Mato path gum (Monacrosporium phymatophagum), Bo Berea Bron near tee (Beauveria brongniartii) , Pekiromyces fuemosoroseus, Beauberia bassiana, Verticillium lecanii, Pekir Mystenuipes (Paec) lomyces tenuipes), Spodoptera litura Nucleopolyhedrovirus, Agrobacterium radiobacter, Bacterium cerevisia biloba, E. Flabus), Bacillus simplex, Trichoderma atroviride, Pseudomonas fluorescens, Coniochilium mini It is preferable to contain tans (Coniothyrium minitans), zucchini yellow mosaic virus (Zucchini yellow mosaic virus), and Bacillus thuringiensis (Bacillus thuringiensis). Cabbage mite (Neoseiulus californicus), Carp mite (Gyneesius liturivorus), Spider mite (Neoseiurus barkeri), Limonica calyx mite (Amblydromalus limonicus) Amblyseius cucumeris, Amblyseius eharai (Euseius sojaensis), Degenerance burdock mites (Amblyseius degenerans), Aphididae (Amblyseius degenerans) Harmonia axyridis, Agricultural thrips (Franklinthrips vespiformis), Akamegashiwaku thrips (Haplotrips brevitubus), Himeka nosago (Propylea japonica) diocoris tenuis, Pyrophorus typicus, yamasoku pheasant (Chrysoperla carnea), giant beetle (Geocoris varius), and the parasitic natural enemies, E. (Dacnusa sibirica), Koreman abachi (Aphidius colemini), Sabakutsuyachibachi (Eretmocerus eremicus), Stigma terrestris (Neochrysocharis formosa), Chabara aphia insomnia It is more preferable to include the common bee (Cotesia vestalis), and the predatory natural enemies, such as Swallowsky spider mite (Amblyseius swirlius mite, Phytoseieulus persimilis) barkeri), Limonica spider mite (Amblydromalus limonicus), Kumeli kasidani (Amblyseius cucumeris), Nyselleragoka tidani (Amblyseius eharai), Kousegekaburidani (Euseisisjaj) Degeneres lance californicus (Amblyseius degenerans), food moth gall midge (Aphidoletes aphidimyza), Thailand Riku Orius (Orius strigicollis), Harmonia axyridis (Harmonia axyridis), Aliga Tashima thrips (Franklinothrips vespiformis), Mallotus japonicus Kedah thrips (Haplothrips brevitubus), Himekamenoko Tento (Propylea japonica), Tobacco turtle (Nesidiocoris tenuis), Black gourd turtle (Pilophorus typicus), Yamatosakae (Chrysoperla carnea), Giant It is further preferred to include a stink bug (Geocoris varius).
 上記のような相乗作用を確認できるものであれば、2種以上の天敵を用いる場合、その組み合わせはいかなるものであってもよい。 As long as the above synergistic effect can be confirmed, when two or more natural enemies are used, any combination thereof may be used.
 本発明において用いられる有害節足動物に対する天敵の放飼量は、捕食性天敵あるいは寄生性天敵の場合は、0.01 ~ 200頭/m2、好ましくは0.1 ~ 100頭/m2、より好ましくは1 ~ 50頭/m2であることができ、天敵微生物の場合は0.1~20kg/ha、好ましくは0.5~10kg/ha、より好もしくは1~5kg/haであることができる。 The amount of natural enemies released to harmful arthropods used in the present invention is 0.01 to 200 / m 2 , preferably 0.1 to 100 / m 2 in the case of predatory or parasitic natural enemies, More preferably, it can be 1 to 50 heads / m 2 , and in the case of natural enemy microorganisms, 0.1 to 20 kg / ha, preferably 0.5 to 10 kg / ha, more preferably 1 to 5 kg / ha. Can do.
 本発明に用いられるスワルスキーカブリダニ、チリカブリダニ、ミヤコカブリダニ、キイカブリダニ、ヘヤカブリダニ、リモニカスカブリダニ、ククメリスカブリダニ、ニセラーゴカブリダニ、コウヅケカブリダニ、デジェネランスカブリダニ、ショクガタマバエ、タイリクヒメハナカメムシ、ナミテントウ、アリガタシマアザミウマ、アカメガシワクダアザミウマ、ヒメカメノコテントウ、タバコカスミカメ、クロヒョウタンカスミカメ、ヤマトクサカゲロウ、オオメカメムシ、オンシツツヤコバチ、イサエアヒメコバチ、ハモグリコマユバチ、コレマンアブラバチ、サバクツヤコバチ、ハモグリミドリヒメコバチ、チャバラアブラコバチ、コナガサムライコマユバチ、パスツーリアペネトランス、チャハマキ顆粒病ウイルス、リンゴコカクモンハマキ顆粒病ウイルス、スタイナーネマカーポカプサエ、スタイナーネマグラセライ、モナクロスポリウムフィマトパガム、ボーベリアブロンニアティ、ペキロマイセスフモソロセウス、ボベリアバシアーナ、バーティーシリウムレカニ、ペキロマイセステヌイペス、ハスモンヨトウ核多角体病ウイルス、アグロバクテリウムラジオバクター、非病原性エルビニアカロトボーラ、バチルスズブチリス、タラロマイセスフラバス、バチルスシンプレクス、トリコデルマアトロビリデ、シュードモナスフルオレッセンス、コニオチリウムミニタンス、ズッキーニ黄斑モザイクウイルス、バチルスチューリンゲンシスは公知の天敵であり、これらの天敵は市販の製剤から得るか、天然に存在し採取されたものや自製されたものを用いることにより得られる。 Swarsky burdock mites, dust mites, mite burdock mites, cuttlefish mites, limonica bursid mites, black spider mites, nisserago burdock mites, mushroom burdock mites, degenerant burdock mites, shrimp flies, terrestrial cabbage mites used in the present invention , Nami Tento, Arigata Thrips, Akamegasiwada Thrips, Himeme no Mokotento, Tobacco Gypsophila, Black Leopard Gecko, Yamatosakae, Omekamemushi, Ontsutsuyakobachi, Isaera Himebachi , Chabara wasp, beak samurai bee, pasturia penetrans, chahamaki granule disease virus, appleko Spider leaf granule disease virus, Steiner nematode Capocapusae, Steiner nematode Graserai, Monacrosporium fimatopagum, Beauvere Abronniati, Pekir Myces fumosoroseus, Boberia Vasiana, Verticillium recani, Pekir Myces Tenuipes, Spodoptera nucleopolyhedrovirus, Agrobacterium radiobacter, Non-pathogenic Erbinia carotobola, Bacillus butyris, Talaromyces flavus, Bacillus simplex, Trichoderma atrovilide, Pseudomonas fluorescens, Coniotylium mini Tansu, zucchini macular mosaic virus, and Bacillus thuringiensis are known natural enemies, and these natural enemies can be obtained from commercial preparations, or can be naturally occurring, collected or self-made. More is obtained.
 本発明では、用いられる天敵の成長速度が有害節足動物の成長速度を上回る環境条件を有する生物的防除方法であることが望ましい。このような環境条件としては、例えば、天敵の発育適温域に含まれる温度条件に適応する作物の栽培型を選択することや、当該温度条件( 具体的には例えば、発育零点以上の温度条件、好ましくは飛翔可能限界温度以上、より好ましくは産卵限界温度以上の温度条件)に施設栽培ハウス内の温度を設定すること等を選択することができ、有害節足動物の個体数よりも天敵の個体数の自然増加率が上回るようにすればよい。なお、あらかじめ前記天敵及び有害節足動物の発育零点、飛翔可能限界温度、産卵限界温度等に係る情報を収集、蓄積しておき、蓄積された各種の情報に基づき有害節足動物の発育よりも前記天敵の発育により適切な温度条件を選択することもできる。
 生物的防除の目的で、天敵の成長速度が有害節足動物の成長速度を上回る環境条件を有するか否かを確認する方法には、経験則に基づくものであってもよく、また定量的な手段だけでなく定性的な手段であってもよい。例えば、上述のような環境条件を有するか否かを確認したり、また農作物当たりに見出される天敵の頭数( 例えば、1 0 - 2 0 花に1 頭以上) 等の存在を調査・観察したり、天敵の放飼後に自然発生した土着天敵の増加を観察・確認してもよい。当該生物的防除方法では、天敵の放飼に際しては、有害節足動物の発生初期段階から1週間間隔で、例えば2 ~ 3 回程度等の複数回放飼して天敵を定着させることも好ましい。
In the present invention, it is desirable that the biological control method has an environmental condition in which the growth rate of natural enemies used exceeds the growth rate of harmful arthropods. As such an environmental condition, for example, selecting a cultivation type of a crop adapted to a temperature condition included in a suitable temperature range for development of natural enemies, the temperature condition (specifically, for example, a temperature condition of a growth zero point or higher, It is possible to choose to set the temperature in the facility cultivation house to a temperature that is preferably higher than the flight limit temperature, more preferably higher than the egg-laying limit temperature, and the natural enemy's individual rather than the number of harmful arthropods. The natural increase rate of numbers should be exceeded. It should be noted that information related to the growth zero of the natural enemies and harmful arthropods, the flight limit temperature, the egg-laying limit temperature, etc. are collected and accumulated in advance, and rather than the development of harmful arthropods based on the accumulated information. Appropriate temperature conditions can be selected depending on the growth of the natural enemy.
For biological control purposes, methods for determining whether natural enemy growth rates have environmental conditions that exceed those of harmful arthropods may be empirical or quantitative. Qualitative means as well as means may be used. For example, whether or not the above environmental conditions are met, and the number of natural enemies found per crop (for example, one or more in 10-20 flowers) is investigated and observed. , You may observe and confirm the increase of natural enemies that naturally occurred after the release of natural enemies. In this biological control method, when releasing natural enemies, it is also preferable to release the natural enemies by releasing them several times, such as about 2 to 3 times, for example, at intervals of one week from the initial stage of occurrence of harmful arthropods.
 本発明における組み合わせ物では、上述したとおり、誘引物質と天敵の組み合わせが、同時にあるいは任意の所望の順番で同一空間となる施設ハウス内あるいは露地に施用されることにより存在し得る。 In the combination according to the present invention, as described above, the combination of the attracting substance and the natural enemy can exist by being applied to the facility house or the open ground that are the same space at the same time or in any desired order.
 本発明の誘引物質と天敵の組み合わせは、具体的には、後述される植物由来のカイロモンおよびその類縁体から選ばれる化合物群よりなる群から選ばれる少なくとも1種の化合物と、後述される天敵よりなる群から選ばれる少なくとも1種の天敵との組み合わせであることができる。誘引物質の組み合わせとしては、
1)スワルスキーカブリダニ、p-アニスアルデヒド
2)スワルスキーカブリダニ、ベンズアルデヒド
3)スワルスキーカブリダニ、ニコチン酸エチル
4)スワルスキーカブリダニ、ゲラニオール
5)スワルスキーカブリダニ、リナロール
6)スワルスキーカブリダニ、ネロール
7)スワルスキーカブリダニ、シトロネロール
8)スワルスキーカブリダニ、o-アニスアルデヒド
9)スワルスキーカブリダニ、β-ファルネセン
10)スワルスキーカブリダニ、アントラニル酸メチル
11)スワルスキーカブリダニ、安息香酸メチル
12)スワルスキーカブリダニ、o-アミノアセトフェノン
13)スワルスキーカブリダニ、o-アニシジン
14)スワルスキーカブリダニ、m-アミノ安息香酸メチル
15)スワルスキーカブリダニ、o-トルイル酸メチル
16)スワルスキーカブリダニ、オイゲノール
17)スワルスキーカブリダニ、3-フェニルプロピルアルデヒド
18)スワルスキーカブリダニ、シンナムアルデヒド
19)スワルスキーカブリダニ、ユーカリプトール
20)スワルスキーカブリダニ、スクアレン
21)スワルスキーカブリダニ、α-ヘキシルシンナムアルデヒド
22)タイリクヒメハナカメムシ、p-アニスアルデヒド
23)タイリクヒメハナカメムシ、ベンズアルデヒド
24)タイリクヒメハナカメムシ、ニコチン酸エチル
25)タイリクヒメハナカメムシ、ゲラニオール
26)タイリクヒメハナカメムシ、リナロール
27)タイリクヒメハナカメムシ、ネロール
28)タイリクヒメハナカメムシ、シトロネロール
29)タイリクヒメハナカメムシ、o-アニスアルデヒド
30)タイリクヒメハナカメムシ、β-ファルネセン
31)タイリクヒメハナカメムシ、アントラニル酸メチル
32)タイリクヒメハナカメムシ、安息香酸メチル
33)タイリクヒメハナカメムシ、o-アミノアセトフェノン
34)タイリクヒメハナカメムシ、o-アニシジン
35)タイリクヒメハナカメムシ、m-アミノ安息香酸メチル
36)タイリクヒメハナカメムシ、o-トルイル酸メチル
37)タイリクヒメハナカメムシ、オイゲノール
38)タイリクヒメハナカメムシ、3-フェニルプロピルアルデヒド
39)タイリクヒメハナカメムシ、シンナムアルデヒド
40)タイリクヒメハナカメムシ、ユーカリプトール
41)タイリクヒメハナカメムシ、スクアレン
42)タイリクヒメハナカメムシ、α-ヘキシルシンナムアルデヒド
43)チリカブリダニ、p-アニスアルデヒド
44)チリカブリダニ、ベンズアルデヒド
45)チリカブリダニ、ニコチン酸エチル
46)チリカブリダニ、ゲラニオール
47)チリカブリダニ、リナロール
48)チリカブリダニ、ネロール
49)チリカブリダニ、シトロネロール
50)チリカブリダニ、o-アニスアルデヒド
51)チリカブリダニ、β-ファルネセン
52)チリカブリダニ、アントラニル酸メチル
53)チリカブリダニ、安息香酸メチル
54)チリカブリダニ、o-アミノアセトフェノン
55)チリカブリダニ、o-アニシジン
56)チリカブリダニ、m-アミノ安息香酸メチル
57)チリカブリダニ、o-トルイル酸メチル
58)チリカブリダニ、オイゲノール
59)チリカブリダニ、3-フェニルプロピルアルデヒド
60)チリカブリダニ、シンナムアルデヒド
61)チリカブリダニ、ユーカリプトール
62)チリカブリダニ、スクアレン
63)チリカブリダニ、α-ヘキシルシンナムアルデヒド
64)ミヤコカブリダニ、p-アニスアルデヒド
65)ミヤコカブリダニ、ベンズアルデヒド
66)ミヤコカブリダニ、ニコチン酸エチル
67)ミヤコカブリダニ、ゲラニオール
68)ミヤコカブリダニ、リナロール
69)ミヤコカブリダニ、ネロール
70)ミヤコカブリダニ、シトロネロール
71)ミヤコカブリダニ、o-アニスアルデヒド
72)ミヤコカブリダニ、β-ファルネセン
73)ミヤコカブリダニ、アントラニル酸メチル
74)ミヤコカブリダニ、安息香酸メチル
75)ミヤコカブリダニ、o-アミノアセトフェノン
76)ミヤコカブリダニ、o-アニシジン
77)ミヤコカブリダニ、m-アミノ安息香酸メチル
78)ミヤコカブリダニ、o-トルイル酸メチル
79)ミヤコカブリダニ、オイゲノール
80)ミヤコカブリダニ、3-フェニルプロピルアルデヒド
81)ミヤコカブリダニ、シンナムアルデヒド
82)ミヤコカブリダニ、ユーカリプトール
83)ミヤコカブリダニ、スクアレン
84)ミヤコカブリダニ、α-ヘキシルシンナムアルデヒド
85)タバコカスミカメ、p-アニスアルデヒド
86)タバコカスミカメ、ベンズアルデヒド
87)タバコカスミカメ、ニコチン酸エチル
88)タバコカスミカメ、ゲラニオール
89)タバコカスミカメ、リナロール
90)タバコカスミカメ、ネロール
91)タバコカスミカメ、シトロネロール
92)タバコカスミカメ、o-アニスアルデヒド
93)タバコカスミカメ、β-ファルネセン
94)タバコカスミカメ、アントラニル酸メチル
95)タバコカスミカメ、安息香酸メチル
96)タバコカスミカメ、o-アミノアセトフェノン
97)タバコカスミカメ、o-アニシジン
98)タバコカスミカメ、m-アミノ安息香酸メチル
99)タバコカスミカメ、o-トルイル酸メチル
100)タバコカスミカメ、オイゲノール
101)タバコカスミカメ、3-フェニルプロピルアルデヒド
102)タバコカスミカメ、シンナムアルデヒド
103)タバコカスミカメ、ユーカリプトール
104)タバコカスミカメ、スクアレン
105)タバコカスミカメ、α-ヘキシルシンナムアルデヒド
106)オンシツツヤコバチ、p-アニスアルデヒド
107)オンシツツヤコバチ、ベンズアルデヒド
108)オンシツツヤコバチ、ニコチン酸エチル
109)オンシツツヤコバチ、ゲラニオール
110)オンシツツヤコバチ、リナロール
111)オンシツツヤコバチ、ネロール
112)オンシツツヤコバチ、シトロネロール
113)オンシツツヤコバチ、o-アニスアルデヒド
114)オンシツツヤコバチ、β-ファルネセン
115)オンシツツヤコバチ、アントラニル酸メチル
116)オンシツツヤコバチ、安息香酸メチル
117)オンシツツヤコバチ、o-アミノアセトフェノン
118)オンシツツヤコバチ、o-アニシジン
119)オンシツツヤコバチ、m-アミノ安息香酸メチル
120)オンシツツヤコバチ、o-トルイル酸メチル
121)オンシツツヤコバチ、オイゲノール
122)オンシツツヤコバチ、3-フェニルプロピルアルデヒド
123)オンシツツヤコバチ、シンナムアルデヒド
124)オンシツツヤコバチ、ユーカリプトール
125)オンシツツヤコバチ、スクアレン
126)オンシツツヤコバチ、α-ヘキシルシンナムアルデヒド
127)コレマンアブラバチ、p-アニスアルデヒド
128)コレマンアブラバチ、ベンズアルデヒド
129)コレマンアブラバチ、ニコチン酸エチル
130)コレマンアブラバチ、ゲラニオール
131)コレマンアブラバチ、リナロール
132)コレマンアブラバチ、ネロール
133)コレマンアブラバチ、シトロネロール
134)コレマンアブラバチ、o-アニスアルデヒド
135)コレマンアブラバチ、β-ファルネセン
136)コレマンアブラバチ、アントラニル酸メチル
137)コレマンアブラバチ、安息香酸メチル
138)コレマンアブラバチ、o-アミノアセトフェノン
139)コレマンアブラバチ、o-アニシジン
140)コレマンアブラバチ、m-アミノ安息香酸メチル
141)コレマンアブラバチ、o-トルイル酸メチル
142)コレマンアブラバチ、オイゲノール
143)コレマンアブラバチ、3-フェニルプロピルアルデヒド
144)コレマンアブラバチ、シンナムアルデヒド
145)コレマンアブラバチ、ユーカリプトール
146)コレマンアブラバチ、スクアレン
147)コレマンアブラバチ、α-ヘキシルシンナムアルデヒド
148)バーティシリウムレカニ、p-アニスアルデヒド
149)バーティシリウムレカニ、ベンズアルデヒド
150)バーティシリウムレカニ、ニコチン酸エチル
151)バーティシリウムレカニ、ゲラニオール
152)バーティシリウムレカニ、リナロール
153)バーティシリウムレカニ、ネロール
154)バーティシリウムレカニ、シトロネロール
155)バーティシリウムレカニ、o-アニスアルデヒド
156)バーティシリウムレカニ、β-ファルネセン
157)バーティシリウムレカニ、アントラニル酸メチル
158)バーティシリウムレカニ、安息香酸メチル
159)バーティシリウムレカニ、o-アミノアセトフェノン
160)バーティシリウムレカニ、o-アニシジン
161)バーティシリウムレカニ、m-アミノ安息香酸メチル
162)バーティシリウムレカニ、o-トルイル酸メチル
163)バーティシリウムレカニ、オイゲノール
164)バーティシリウムレカニ、3-フェニルプロピルアルデヒド
165)バーティシリウムレカニ、シンナムアルデヒド
166)バーティシリウムレカニ、ユーカリプトール
167)バーティシリウムレカニ、スクアレン
168)バーティシリウムレカニ、α-ヘキシルシンナムアルデヒド
169)パスツーリアペネトランス、p-アニスアルデヒド
170)パスツーリアペネトランス、ベンズアルデヒド
171)パスツーリアペネトランス、ニコチン酸エチル
172)パスツーリアペネトランス、ゲラニオール
173)パスツーリアペネトランス、リナロール
174)パスツーリアペネトランス、ネロール
175)パスツーリアペネトランス、シトロネロール
176)パスツーリアペネトランス、o-アニスアルデヒド
177)パスツーリアペネトランス、β-ファルネセン
178)パスツーリアペネトランス、アントラニル酸メチル
179)パスツーリアペネトランス、安息香酸メチル
180)パスツーリアペネトランス、o-アミノアセトフェノン
181)パスツーリアペネトランス、o-アニシジン
182)パスツーリアペネトランス、m-アミノ安息香酸メチル
183)パスツーリアペネトランス、o-トルイル酸メチル
184)パスツーリアペネトランス、オイゲノール
185)パスツーリアペネトランス、3-フェニルプロピルアルデヒド
186)パスツーリアペネトランス、シンナムアルデヒド
187)パスツーリアペネトランス、ユーカリプトール
188)パスツーリアペネトランス、スクアレン
189)パスツーリアペネトランス、α-ヘキシルシンナムアルデヒド
190)ボーベリアバシアーナ、p-アニスアルデヒド
191)ボーベリアバシアーナ、ベンズアルデヒド
192)ボーベリアバシアーナ、ニコチン酸エチル
193)ボーベリアバシアーナ、ゲラニオール
194)ボーベリアバシアーナ、リナロール
195)ボーベリアバシアーナ、ネロール
196)ボーベリアバシアーナ、シトロネロール
197)ボーベリアバシアーナ、o-アニスアルデヒド
198)ボーベリアバシアーナ、β-ファルネセン
199)ボーベリアバシアーナ、アントラニル酸メチル
200)ボーベリアバシアーナ、安息香酸メチル
201)ボーベリアバシアーナ、o-アミノアセトフェノン
202)ボーベリアバシアーナ、o-アニシジン
203)ボーベリアバシアーナ、m-アミノ安息香酸メチル
204)ボーベリアバシアーナ、o-トルイル酸メチル
205)ボーベリアバシアーナ、オイゲノール
206)ボーベリアバシアーナ、3-フェニルプロピルアルデヒド
207)ボーベリアバシアーナ、シンナムアルデヒド
208)ボーベリアバシアーナ、ユーカリプトール
209)ボーベリアバシアーナ、スクアレン
210)ボーベリアバシアーナ、α-ヘキシルシンナムアルデヒド
211)バチルスチューリンゲンシス、p-アニスアルデヒド
212)バチルスチューリンゲンシス、ベンズアルデヒド
213)バチルスチューリンゲンシス、ニコチン酸エチル
214)バチルスチューリンゲンシス、ゲラニオール
215)バチルスチューリンゲンシス、リナロール
216)バチルスチューリンゲンシス、ネロール
217)バチルスチューリンゲンシス、シトロネロール
218)バチルスチューリンゲンシス、o-アニスアルデヒド
219)バチルスチューリンゲンシス、β-ファルネセン
220)バチルスチューリンゲンシス、アントラニル酸メチル
221)バチルスチューリンゲンシス、安息香酸メチル
222)バチルスチューリンゲンシス、o-アミノアセトフェノン
223)バチルスチューリンゲンシス、o-アニシジン
224)バチルスチューリンゲンシス、m-アミノ安息香酸メチル
225)バチルスチューリンゲンシス、o-トルイル酸メチル
226)バチルスチューリンゲンシス、オイゲノール
227)バチルスチューリンゲンシス、3-フェニルプロピルアルデヒド
228)バチルスチューリンゲンシス、シンナムアルデヒド
229)バチルスチューリンゲンシス、ユーカリプトール
230)バチルスチューリンゲンシス、スクアレン
231)バチルスチューリンゲンシス、α-ヘキシルシンナムアルデヒド
を挙げることができる。上記1)~231)に列挙された天敵及び誘引物質は、本発明の目的に沿う効果を奏する限り、それぞれ、2種以上を併用することもできる。
Specifically, the combination of the attractant of the present invention and the natural enemy is based on at least one compound selected from the group consisting of compounds derived from plant-derived kairomones and analogs thereof described below, and natural enemies described below. It can be a combination with at least one natural enemy selected from the group consisting of: As a combination of attractants,
1) Swarsky burdock mites, p-anisaldehyde 2) Swarsky burdock mites, benzaldehyde 3) Swarsky burdock mites, ethyl nicotinate 4) Swarsky burdock mites, geraniol 5) Swarsky burdock mites, linalool 6) Swarsky burdock mites, Nellore 7) Swar 8) Swarsky burdock mite, o-anisaldehyde 9) Swarsky burdock mite, β-farnesene 10) Swarsky burdock mite, methyl anthranilate 11) Swarsky burdock mite, methyl benzoate 12) Swarsky burdock mite, o-amino Acetophenone 13) Swarsky burdock mite, o-anisidine 14) Swarsky burdock mite, methyl m-aminobenzoate 15) Swarsky burdock mite, o-to Methyl ylate 16) Swarsky burdock mites, Eugenol 17) Swarsky burdock mites, 3-phenylpropyl aldehyde 18) Swarsky burdock mites, cinnamaldehyde 19) Swarsky burdock mites, eucalyptoles 20) Swarsky burdock mites, Squalene 21) Swarsky burdock mites , Α-hexylcinnamaldehyde 22) Tailflower spruce, p-anisaldehyde 23) Tailhead spruce bug, benzaldehyde 24) Tyler spruce bug, ethyl nicotinate 25) Tyler spruce bug, geraniol 26) Linalool 27) terrestrial beetle, nerol 28) terrestor elegans, citronellol 29) terrestor elegans, o-a Sudealdehyde 30) Tailworm, B-farnesene 31) Tailflower, Streptomyces, methyl anthranilate 32) Tailworm, Streptomyces, methyl benzoate 33) Taillet, Stylus, o-aminoacetophenone 34) o-anisidine 35) Tail-spotted beetle, methyl m-aminobenzoate 36) Tail-spotted beetle, methyl o-toluate 37) Tail-spotted beetle, Eugenol 38) Tail-spotted beetle, 3-phenylpropylaldehyde 39) Timber winged beetle, cinnamaldehyde 40) Timber winged beetle, Eucalyptole 41) Timber winged beetle, squalene 42) Timber horned beetle, α-hexylcinnam Rudehydr 43) dust mites, p-anisaldehyde 44) dust mites, benzaldehyde 45) dust mites, ethyl nicotinate 46) dust mites, geraniol 47) dust mites, linalool 48) dust mites, citronellol 50) dust mites, o-anisaldehyde 51 ) Dust mites, β-farnesene 52) dust mites, methyl anthranilate 53) dust mites, methyl benzoate 54) dust mites, o-aminoacetophenone 55) dust mites, o-anisidine 56) dust mites, methyl m-aminobenzoate 57) dust mites, o -Methyl toluate 58) dust mite, eugenol 59) dust mite, 3-phenylpropyl al Dehydr 60) dust mite, cinnamaldehyde 61) dust mite, eucalyptol 62) dust mite, squalene 63) dust mite, α-hexylcinnamaldehyde 64) Miyako dust mite, p-anisaldehyde 65) Miyako dust mite, benzaldehyde 66) Miyako dust mite, nicotinic acid Ethyl 67) Miyako burdock mite, geraniol 68) Miyako burdock mite, linalool 69) Miyako burdock mite, nerol 70) Miyako burdock mite, citronellol 71) Miyako burdock mite, o-anisaldehyde 72) Miyako burdock mite, β-farnesene 73) Miyako burdock mite, methyl anthranilate 74) Miyako burdock mite, methyl benzoate 75) Miyako burdock mite, o-aminoacetophenone 76) Miyako burdock mite , O-anisidine 77) Miyako burdock mite, methyl m-aminobenzoate 78) Miyako burdock mite, o-toluoyl methyl 79) Miyako burdock mite, Eugenol 80) Miyako burdock mite, 3-phenylpropylaldehyde 81) Miyako burdock mite, cinnamaldehyde 82) Miyako burdock mite, eucalyptol 83) Miyako burdock mite, squalene 84) Miyako burdock mite, α-hexylcinnamaldehyde 85) Tobacco turtle, p-anisaldehyde 86) Tobacco turtle, Benzaldehyde 87) Tobacco turtle, Ethyl nicotinate 88) Tobacco mist Geraniol 89) tobacco turtle, linalool 90) tobacco turtle, nerol 91) tobacco turtle, citronellol 92) tobacco turtle, o-anis Rudehydr 93) tobacco turtle, β-farnesene 94) tobacco turtle, methyl anthranilate 95) tobacco turtle, methyl benzoate 96) tobacco turtle, o-aminoacetophenone 97) tobacco turtle, o-anisidine 98) tobacco turtle, m-amino Methyl benzoate 99) Tobacco turtles, methyl o-toluate 100) Tobacco turtles, Eugenol 101) Tobacco turtles, 3-phenylpropyl aldehyde 102) Tobacco turtles, Cinnamaldehyde 103) Tobacco turtles, Eucalyptol 104) Tobacco turtles, Squalene 105) Tobacco waste turtle, α-hexylcinnamaldehyde 106) On-site bee, p-anisaldehyde 107) On-site bee, benzal Hyde 108) Onsatsume wasp, Ethyl nicotinate 109) Onsatsume wasp, Geraniol 110) Onsatsume wasp, Linalool 111) Onsatsume wasp, Nellore 112) Onsatsume wasp, Citronellol 113) Onsatsuyase o-anisaldehyde 114) Onsatsuma bee, β-farnesene 115) Onsatsume wasp, methyl anthranilate 116) Onsatsume wasp, methyl benzoate 117) Onsatsume bee, o-aminoacetophenone 118) Onsatsuya Bees, o-anisidine 119) on-site wasps, methyl m-aminobenzoate 120) on-site wasps, methyl o-toluate 121) on-site wasps, eugenol 122) on-site wasps, 3-phenylpropi Aldehyde 123) Onsatsuma wasp, cinnamaldehyde 124) Onsatsume wasp, eucalyptol 125) Onsatsume wasp, squalene 126) Onsatsuya wasp, α-hexylcinnamaldehyde 127) Coleman abradhy, p-anisaldehyde 128) Coleman abachi, benzaldehyde 129) Coleman abrachi, ethyl nicotinate 130) Coleman abrachi, geraniol 131) Coleman abachi, linalool 132) Coleman abachi, nerol 133) Coleman abachi, citronellol 134) o-anisaldehyde 135) Coleman abrabic, β-farnesene 136) Coleman abrachi, methyl anthranilate 137) Coleman abrachi, benzoic acid 138) Coleman abrachi, o-aminoacetophenone 139) Coleman abrachi, o-anisidine 140) Coleman abrachi, methyl m-aminobenzoate 141) Coleman abrachi, methyl o-toluate 142) Coleman abrachi, eugenol 143) Coleman abachi, 3-phenylpropylaldehyde 144) Coleman abrachi, cinnamaldehyde 145) Coleman abachi, eucalyptol 146) Coleman abachi, squalene 147) Coleman abachi, α-hexylcinnamaldehyde 148) Verticiliu Mule crani, p-anisaldehyde 149) Verticillium regani, benzaldehyde 150) Verticillium regani, ethyl nicotinate 151) Verticillium regani, gerani All 152) Verticillium recani, linalool 153) Verticillium recani, nerol 154) Verticillium recani, citronellol 155) Verticillium recani, o-anisaldehyde 156) Verticillium recani, β-farnesene 157) Verticillium Mule crani, methyl anthranilate 158) Verticillium recani, methyl benzoate 159) Verticillium recani, o-aminoacetophenone 160) Verticillium recani, o-anisidine 161) Verticillium recani, methyl m-aminobenzoate 162) Verticillium recani, methyl o-toluate 163) Verticillium recani, Eugenol 164) Verticillium recani, 3-phenylpropylaldehyde 165) Vertici Umlekani, cinnamaldehyde 166) Verticillium recani, Eucalyptol 167) Verticillium recani, squalene 168) Verticillium recani, α-hexylcinnamaldehyde 169) Pasteuria penetrans, p-anisaldehyde 170) Pastu Rear Penetrance, Benzaldehyde 171) Pasteuria Penetrance, Ethyl Nicotinate 172) Pasteuria Penetrance, Geraniol 173) Pasteuria Penetrance, Linalool 174) Pasteuria Penetrance, Nerol 175) Pasteuria Penetrance Citronellol 176) Pasteuria penetrans, o-anisaldehyde 177) Pasteuria penetrans, β-farnesene 178) Pasteuria penetrans, anthranils Methyl 179) Pasteuria penetrans, methyl benzoate 180) Pasteuria penetrans, o-aminoacetophenone 181) Pasteuria penetrans, o-anisidine 182) Pasteuria penetrans, methyl m-aminobenzoate 183 ) Pasteuria penetrans, methyl o-toluate 184) Pasteuria penetrans, Eugenol 185) Pasteuria penetrans, 3-phenylpropylaldehyde 186) Pasteuria penetrans, cinnamaldehyde 187) Pasteuria pene Trans, eucalyptol 188) Pasteuria penetrans, Squalene 189) Pasteuria penetrans, α-hexylcinnamaldehyde 190) Boberia vasiana, p-anisaldehyde 191) Boberia vasia Benzaldehyde 192) Beauveria basiana, ethyl nicotinate 193) Beauveria basiana, geraniol 194) Beauveria basiana, linalool 195) Beauberia basiana, nerol 196) Beauberia basiana, citronellol 197) Beauveria basiana, o-anisaldehyde 198) Boberia Vaciana, β-farnesene 199) Boberia Vaciana, methyl anthranilate 200) Boberia Vaciana, methyl benzoate 201) Boberia Vaciana, o-aminoacetophenone 202) Boberia Vaciana, o-anisidine 203) Boberia Vashi Arna, methyl m-aminobenzoate 204) Booberia vaciana, methyl o-toluate 205) Booberia vaciana, Eugenol 2 06) Boberia Vaciana, 3-Phenylpropylaldehyde 207) Boberia Vaciana, Cinnamaldehyde 208) Boberia Vaciana, Eucalyptoll 209) Boberia Vaciana, Squalene 210) Boberia Vaciana, α-Hexylcinnamaldehyde 211) Bacillus Thuringien Cis, p-anisaldehyde 212) Bacillus thuringiensis, benzaldehyde 213) Bacillus thuringiensis, ethyl nicotinate 214) Bacillus thuringiensis, geraniol 215) Bacillus thuringiensis, linalool 216) Bacillus thuringiensis, nerol 217) Bacillus thuringiensis, citronellol 218) Bacillus thuringiensis, o-anisaldehyde 219) Ba Ruthuringiensis, β-farnesene 220) Bacillus thuringiensis, methyl anthranilate 221) Bacillus thuringiensis, methyl benzoate 222) Bacillus thuringiensis, o-aminoacetophenone 223) Bacillus thuringiensis, o-anisidine 224) Bacillus thuringiensis, Methyl m-aminobenzoate 225) Bacillus thuringiensis, methyl o-toluate 226) Bacillus thuringiensis, Eugenol 227) Bacillus thuringiensis 228) Bacillus thuringiensis, cinnamaldehyde 229) Bacillus thuringiensis, Eucalyptus Putol 230) Bacillus thuringiensis, squalene 231) Bacillus thuringe Mention may be made of cis, the α- hexyl cinnamaldehyde. Two or more natural enemies and attractants listed in 1) to 231) can be used in combination as long as the effects of the present invention are achieved.
 有害節足動物は、誘引物質を設置した捕獲器に捕獲された生物数を計測することで調査することができる。推定された個体群密度に基づいて、防除の必要性が判断される。有害節足動物の大量の捕獲は、本発明によって提供される、有害節足動物除去の方法を必要とする。反対に、有害節足動物が低密度である場合、必ずしも迅速な防除を必要としない。 有害 Harmful arthropods can be investigated by counting the number of organisms captured in the trap with the attractant. Based on the estimated population density, the necessity for control is determined. Massive capture of harmful arthropods requires the method of harmful arthropod removal provided by the present invention. Conversely, if the harmful arthropod is of low density, it does not necessarily require rapid control.
 例えば、本発明の誘引物質は、必要により製剤化して、捕獲器内に設置することができる。このような捕獲器は、誘引物質の有効量を放出するように設計されている。当該捕獲器は、有害節足動物が発生、または発生が予測される場所に設置される。誘引物質の匂いは、有害節足動物を捕獲器に誘引するので、その後、例えば、有害節足動物に対して致死効果を示す殺虫剤を捕獲器内に予め設置しておくことによって、捕獲器内に誘引された有害節足動物を捕殺することができる。あるいは捕獲器内またはその近傍に、粘着板を設置することで、誘引、捕殺することも可能である。本発明の誘引物質と配合可能な有効殺虫活性成分の例を以下に記載する。 For example, the attractant of the present invention can be formulated as necessary and placed in a trap. Such traps are designed to release an effective amount of attractant. The trap is installed in a place where harmful arthropods are generated or expected to occur. The odor of the attractant attracts harmful arthropods to the trap, and then, for example, by pre-installing an insecticide that shows a lethal effect on the harmful arthropods in the trap, It is possible to kill harmful arthropods attracted inside. Alternatively, it is possible to attract and kill by installing an adhesive plate in or near the trap. Examples of effective insecticidal active ingredients that can be combined with the attractant of the present invention are described below.
 有効殺虫活性成分:
 o-エチル o-4-ニトロフェニル フェニルホスホノチオアート(EPN)、アセフェート(acephate)、イソキサチオン(isoxathion)、イソフェンホス(isofenphos)、イソプロカルブ(isoprocarb)、エトリムホス(etrimfos)、オキシデプロホス(oxydeprofos)、キナルホス(quinalphos)、キャドサホス(cadusafos)、クロルエトキシホス(chlorethoxyfos)、クロルピリホス(chlorpyrifos)、クロルピリホス-メチル(chlorpyrifos-methyl)、クロロフェンビンホス(chlorofenvinphos)、サリチオン(salithion)、シアノホス(cyanophos)、ジスルホトン(disulfoton)、ジメトエート(dimethoate)、スルプロホス(sulprofos)、ダイアジノン(diazinon)、チオメトン(thiometon)、テトラクロルビンホス(tetrachlorvinphos)、テブピリムホス(tebupirimfos)、トリクロルホン(trichlorphon)、ネイルド(naled)、バミドチオン(vamdothion)、ピラクロホス(pyraclophos)、ピリダフェンチオン(pyridafenthion)、ピリミホス-メチル(pirimiphos-methyl)、フェニトロチオン(fenitrothion)、フェンチオン(fenthion)、フェントエート(phenthoate)、ブタチオホス(butathiofos)、プロチオホス(prothiofos)、プロパホス(propaphos)、プロフェノホス(profenofos)、ベンクロチアズ(benclothiaz)、ホサロン(phosalone)、ホスチアゼート(fosthiazate)、マラチオン(malathion)、メチダチオン(methidathion)、メトルカルブ(metolcarb)、モノクロトホス(monocrotophos)、フェノブカルブ(BPMC)、3,5-キシリルN-メチルカーバメート(XMC)、アラニカルブ(alanycarb)、エチオフェンカルブ(ethiofencarb)、カルバリル(carbaryl)、カルボスルファン(carbosulfan)、カルボフラン(carbofuran)、キシリルカルブ(xylylcarb)、クロエトカルブ(cloethocarb)、チオジカルブ(thiodicarb)、トリアゼメイト(triazamate)、ピリミカーブ(pirimicarb)、フェノキシカーブ(fenoxycarb)、フェノチオカルブ(fenothiocarb)、フラチオカルブ(furathiocarb)、プロポクスル(propoxur)、ベンダイオカルブ(bendiocarb)、ベンフラカルブ(benfuracarb)、メソミル(methomyl)、アクリナトリン(acrinathrin)、イミプロトリン(imiprothrin)、エトフェンプロックス(ethofenprox)、シクロプロトリン(cycloprothrin)、シグマ-サイパーメスリン(sigma-cypermethrin)、シハロトリン(cyhalothrin)、シフルトリン(cyfluthrin)、シペルメトリン(cypermethrin)、シラフルオフェン(silafluofen)、テフルトリン(tefluthrin)、デルタメトリン(deltamethrin)、トラロメトリン(tralomethrin)、フェンバレレート(fenvalerate)、フェンプロパトリン(fenpropathrin)、フルシスリネート(flucythrinate)、フルバリネート(fluvalinate)、フルフェンプロックス(flufenoprox)、フルプロキシフェン(fluproxyfen)、プロフルトリン(profluthrn)、ベータ-シフルトリン(beta-cyfluthrin)、ベンフルスリン(benfluthrin)、ペルメトリン(permethrin)、カルタップ(cartap)、チオシクラム(thiocyclam)、ベンスルタップ(bensultap)、アベルメクチン(avermectin)、エマメクチンベンゾエート(emamectin-benzoate)、クロルフルアズロン(chlorfluazuron)、シロマジン(cyromazine)、ジアフェンチウロン(diafenthiuron)、ジクロルボス(dichlorvos)、ジフルベンズロン(diflubenzuron)、スピノシン(spynosyn)、スピロメシフェン(spiromesifen)、テフルベンズロン(teflubenzuron)、テブフェノジド(tebufenozide)、ハイドロプレン(hydroprene)、バニリプロール(vaniliprole)、ピメトロジン(pymetrozine)、ピリプロキシフェン(pyriproxyfen)、フィプロニル(fipronil)、フルフェノクスロン(flufenoxuron)、ブプロフェジン(buprofezin)、ヘキサフルムロン(hexaflumuron)、ミルベマイシン(milbemycin)、ルフェヌロン(lufenuron)、クロルフェナピル (chlorphenapyr)、ピリダリル(pyridalyl)、フルフェンジアミド(flufendiamide)、SI-0009、メトフルトリン(metofluthrin)、ノビフルムロン(noviflumuron)、ジメフルトリン(dimefluthrin)、シフルメトフェン(cyflumetofen)、ピラフルプロール(pyrafluprole)、ピリプロール(pyriprole)
Effective insecticidal active ingredient:
o-ethyl o-4-nitrophenyl phenylphosphonothioate (EPN), acephate, isoxathion, isofenphos, isoprocarb, etrimfos, oxydeprofos, oxydeprofos (Quinalphos), cadusafos, chlorethoxyphos, chlorpyrifos, chlorpyrifos-methyl, chlorofenvinphos, alithifo Difofoton, dimethoate, sulprofos, diazinon, thiomethon, tetrachlorvinphos, tebupyrimfos, tebupirimfos, tebupirimfos vamdotion, pyraclophos, pyridafenthion, pyrimiphos-methyl, fenitrothion, fenthion, phenthate, buthothioate Buththiofos, prothiofos, propopafos, profenofos, beclothiaz, fosalone, fothiazate, malthion, malathion (malthion) Monocrotophos, fenocarb (BPMC), 3,5-xylyl N-methyl carbamate (XMC), alanic carb, etiofencarb, carbaryl, carbosulfan, carbofuran carbofuran, xylylcarb, cloethocarb, thiodicarb, triazemate, triazemate, pirimicarb, phenoxycarb, fenoxycurb Iodocarb, benfuracarb, mesomyl, acrinathrin, imiprothrin, etofenprox, cycloproton (cycloproline) thrin), sigma-cypermethrin, cyhalothrin, cyfluthrin, cypermethrin, sylflufen, tefluthrin, tefluthrin, tefluthrin, tefluthrin (Fenvalerate), fenpropatrin, flucisphosphate, fluvalinate, flufenprox, fluproxyfen, profluthrin ( profluthrn), beta-cyfluthrin, benfluthrin, permethrin, cartap, thiocyclam, benzometic, benzmuctin Chlorfluazuron, cyromazine, diafenthiuron, dichlorvos, diflubenzuron, spinosyn, spiromesifen ), Teflubenzuron, tebufenozide, hydroprene, vaniliprole, ferrobile, fropronyl, piroproxen. ), Hexaflumuron, milbemycin, lufenuron, chlorphenapyr, pyridalyl, flufendiamide, SI-0009, methof Trinh (metofluthrin), noviflumuron (noviflumuron), dimefluthrin (dimefluthrin), cyflumetofen (cyflumetofen), pyrafluprole (pyrafluprole), pyriprole (pyriprole)
 また、本発明においては例えば、他の殺虫活性成分、共力剤(例、ピペロニル ブトキシド(piperonyl butoxide)、セサメックス スルホキシド(sesamex sulfoxide)、MGK 264、N-デシルイミダゾール(N-declyimidazole)、WARF-アンチレジスタント(WARF-antiresistant)、TBPT、TPP、IBP、PSCP、CH3I、t-フェニルブテノン(t-phenylbutenone)、マレイン酸ジエチル(diethylmaleate)、DMC、FDMC、ETP、ETN)、と誘引物質を配合し、適宜使用することも可能である。 Further, in the present invention, for example, other insecticidal active ingredients, synergists (eg piperonyl butoxide, sesamex sulfoxide), MGK 264, N-decylimidazole, N-decylimidazole, Contains an inducer and a resistant (WARF-antiresistant), TBPT, TPP, IBP, PSCP, CH3I, t-phenylbutenone, diethyl maleate, DMC, FDMC, ETP, ETN However, it can be used as appropriate.
 誘引物質の製剤化の際に用いられる固体担体としては、例えば、カオリンクレー、アッタパルジャイトクレー、ベントナイト、モンモリロナイト、酸性白土、パイロフィライト、タルク、珪藻土及び方解石等の鉱物、トウモロコシ穂軸粉、クルミ殻粉等の天然有機物、尿素等の合成有機物、炭酸カルシウム、硫酸アンモニウム等の塩類、合成含水酸化珪素等の合成無機物等からなる微粉末あるいは粒状物等が挙げられ、液体担体としては、例えばキシレン、アルキルベンゼン、メチルナフタレン等の芳香族炭化水素類、2-プロパノール、エチレングリコール、プロピレングリコール、エチレングリコールモノエチルエーテル等のアルコール類、アセトン、シクロヘキサノン、イソホロン等のケトン類、ダイズ油、綿実油等の植物油、石油系脂肪族炭化水素類、エステル類、ジメチルスルホキシド、アセトニトリル並びに水が挙げられる。
 界面活性剤としては、例えば、アルキル硫酸エステル塩、アルキルアリールスルホン酸塩、ジアルキルスルホコハク酸塩、ポリオキシエチレンアルキルアリールエーテルリン酸エステル塩、リグニンスルホン酸塩及びナフタレンスルホネートホルムアルデヒド重縮合物等の陰イオン界面活性剤、ポリオキシエチレンアルキルアリールエーテル、ポリオキシエチレンアルキルポリオキシプロピレンブロックコポリマー及びソルビタン脂肪酸エステル等の非イオン界面活性剤、並びにアルキルトリメチルアンモニウム塩等の陽イオン界面活性剤が挙げられる。
 その他の製剤用補助剤としては、例えば、ポリビニルアルコール、ポリビニルピロリドン等の水溶性高分子、アラビアゴム、アルギン酸及びその塩、CMC(カルボキシメチルセルロ-ス)及びザンサンガム等の多糖類、アルミニウムマグネシウムシリケート、アルミナゾル等の無機物、防腐剤、着色剤並びにPAP(酸性リン酸イソプロピル)、BHT等の安定化剤が挙げられる。
Examples of the solid carrier used in formulating the attractant include kaolin clay, attapulgite clay, bentonite, montmorillonite, acid clay, pyrophyllite, talc, diatomaceous earth and calcite, corn cob flour, Examples include a natural organic material such as walnut shell powder, a synthetic organic material such as urea, a salt such as calcium carbonate and ammonium sulfate, a fine powder or a granular material composed of a synthetic inorganic material such as synthetic hydrous hydroxide, and the liquid carrier includes, for example, xylene , Aromatic hydrocarbons such as alkylbenzene and methylnaphthalene, alcohols such as 2-propanol, ethylene glycol, propylene glycol and ethylene glycol monoethyl ether, ketones such as acetone, cyclohexanone and isophorone, vegetable oils such as soybean oil and cottonseed oil , Petroleum Aliphatic hydrocarbons, esters, dimethylsulfoxide, acetonitrile and water.
Surfactants include, for example, anions such as alkyl sulfate esters, alkylaryl sulfonates, dialkyl sulfosuccinates, polyoxyethylene alkylaryl ether phosphate esters, lignin sulfonates and naphthalene sulfonate formaldehyde polycondensates. Nonionic surfactants such as surfactants, polyoxyethylene alkyl aryl ethers, polyoxyethylene alkyl polyoxypropylene block copolymers and sorbitan fatty acid esters, and cationic surfactants such as alkyltrimethylammonium salts.
Other formulation adjuvants include, for example, water-soluble polymers such as polyvinyl alcohol and polyvinyl pyrrolidone, gum arabic, alginic acid and its salts, polysaccharides such as CMC (carboxymethyl cellulose) and xanthan gum, aluminum magnesium silicate, Examples include inorganic substances such as alumina sol, preservatives, colorants, and stabilizers such as PAP (isopropyl acid phosphate) and BHT.
 本発明は有害節足動物を誘引し捕獲することで、下記の植物に対して摂食、吸汁等の加害を行う有害節足動物(例えば、有害昆虫および有害ダニなどの有害節足動物)による加害から植物を保護することができる。 The present invention is based on harmful arthropods (for example, harmful arthropods such as harmful insects and harmful ticks) that attract and capture harmful arthropods and cause feeding, sucking, etc. Plants can be protected from harm.
 本発明の誘引物質と天敵を併用した予察方法および防除方法は、畑、水田、芝生、果樹園などの農耕地または非農耕地用にて使用することが可能である。また、本発明は、以下に挙げられる「植物」などを栽培する農耕地等において、該当植物等に対して薬害を与えることなく、当該農耕地の有害節足動物を防除するために使用することができる。 The prediction method and control method using the attractant and natural enemy of the present invention can be used in farmland such as fields, paddy fields, lawns, orchards, or non-agricultural land. In addition, the present invention is used to control harmful arthropods in the farmland without causing any phytotoxicity in the farmland where the “plants” listed below are cultivated. Can do.
 農作物:トウモロコシ(Zea mays)、イネ(Oryza sativa)、コムギ(Triticum aestivum)、オオムギ(Hordeum vulgare)、ライムギ(Secale cereale)、エンバク(Avena sativa)、ソルガム(Sorghum bicolor (L.) Moench)、ワタ(Gossypium arboreum)、ダイズ(Glycine max)、ピーナッツ(Arachis hypogaea)、ソバ(Fagopyrum esculentum)、テンサイ(Beta vulgaris ssp. vulgaris)、ナタネ(Brassica rapa var.amplexicaulis)、ヒマワリ(Helianthus annuus)、サトウキビ(Saccharum officinarum)、タバコ(Nicotiana tabacum)等。
 野菜;ナス科野菜(ナス(Solanum melongena)、トマト(Solanum lycopersicum)、ピーマン(Capsicum annuum var. angulosum)、トウガラシ(Capsicum annuum)、ジャガイモ(Solanum tuberosum)等)、ウリ科野菜(キュウリ(Cucumis sativus)、カボチャ(Cucurbita maxima)、ズッキーニ(Cucurbita pepo)、スイカ(Citrullus lanatus)、メロン(Cucumis melo)等)、アブラナ科野菜(ダイコン(Raphanus sativus var. longipinnatus)、カブ(Brassica rapa L. var. glabra)、セイヨウワサビ(Armoracia rusticana)、コールラビ(Brassica oleracea var. gongylodes)、ハクサイ(Brassica rapa var. pekinensis)、キャベツ(Brassica Oleracea)、カラシナ(Brassica juncea)、ブロッコリー(Brassica oleracea var. italica)、カリフラワー(Brassica oleracea var. botrytis)、アブラナ(Brassica napus)等)、キク科野菜(ゴボウ(Arctium lappa)、シュンギク(Glebionis coronaria)、アーティチョーク(Cynara scolymus)、レタス(Lactuca sativa)等)、ユリ科野菜(ネギ(Allium fistulosum)、タマネギ(Allium cepa)、ニンニク(Allium sativum)、アスパラガス(Asparagus officinalis)等)、セリ科野菜(ニンジン(Daucus carota)、パセリ(Petroselinum neapolitanum)、セロリ(Apium graveolens var. dulce)、アメリカボウフウ(Pastinaca sativa)等)、アカザ科野菜(ホウレンソウ(Spinacia oleracea)、フダンソウ(Beta vulgaris var. cicla)等)、シソ科野菜(シソ(Perilla frutescens var. crispa)、ミント(Mentha piperita)、バジル(Ocimum basilicum)等)、イチゴ(Fragaria ananassa Duchesne)、サツマイモ(Ipomoea batatas)、ヤマノイモ(Dioscorea japonica)、サトイモ(Colocasia esculenta)等。
 果樹:仁果類(リンゴ(Malus pumila)、セイヨウナシ(Pyrus communis)、ニホンナシ(Pyrus pyrifolia var. culta)、カリン(Chaenomeles sinensis)、マルメロ(Cydonia oblonga)等)、核果類(モモ(Amygdalus persica)、スモモ(Prunus salicina)、ネクタリン(Amygdalus persica var. nectarina)、ウメ(Prunus mume)、オウトウ(Cerasus avium)、アンズ(Prunus armeniaca)、プルーン(Prunus armeniaca)等)、カンキツ類(ウンシュウミカン(Citrus unshiu)、オレンジ(Citrus sinensis)、レモン(Citrus limon)、ライム(Citrus aurantifolia)、グレープフルーツ(Citrus paradisi)等)、堅果類(クリ(Castanea crenata)、クルミ(Juglans mandshurica var. sieboldiana)、ハシバミ(Corylus avellana)、アーモンド(Prunus dulcis)、ピスタチオ(Pistacia vera)、カシューナッツ(Anacardium occidentale)、マカダミアナッツ(Macadamia integrifolia)等)、液果類(ブルーベリー(Vaccinium corymbosum)、クランベリー(Vaccinium macrocarpon)、ブラックベリー(Rubus fruticosus)、ラズベリー(Rubus idaeus)等)、ブドウ(Vitis labrusca)、カキ(Diospyros kaki Thunberg)、オリーブ(Olea europaea)、ビワ(Eriobotrya japonica)、バナナ(Ensete ventricosum)、コーヒー(Coffea arabica)、ナツメヤシ(Phoenix dactylifera)、ココヤシ(Cocos nucifera)、アブラヤシ(Elaeis oleifera)等。
 果樹以外の樹木:チャ(Camellia sinensis)、クワ(Morus alba)、花木類(サツキ(Rhododendron indicum)、ツバキ(Camellia japonica)、アジサイ(Hydrangea macrophylla)、サザンカ(Camellia sasanqua)、シキミ(Illicium anisatum)、サクラ(Cerasus xyedoensis)、ユリノキ(Liriodendron tulipifera)、サルスベリ(Lagerstroemia indica)、キンモクセイ(Osmanthus fragrans var. aurantiacus)等)、街路樹(トネリコ(Fraxinus japonica)、カバノキ(Betula platyphylla var.japonica)、ハナミズキ(Benthamidia florida)、ユーカリ(Eucalyptus globulus)、イチョウ(Ginkgo biloba)、ライラック(Syringa vulgaris)、カエデ(Acer palmatum)、カシ(Quercus myrsinaefolia)、ポプラ(Populus angulata)、ハナズオウ(Cercis chinensis)、フウ(Liquidambar formosana)、プラタナス(Platanus orientalis)、ケヤキ(Zelkova serrata)、クロベ(Thuja standishii)、モミノキ(Abies firma)、ツガ(Tsuga sieboldii)、ネズ(Juniperus rigida)、マツ(Pinus densiflora)、トウヒ(Picea jezoensis var. hondoensis)、イチイ(Taxus cuspidata)、ニレ(Ulmus davidiana var. japonica)、トチノキ(Aesculus turbinata)等)、サンゴジュ(Viburnum odoratissimum var. awabuki)、イヌマキ(Podocarpus macrophyllus)、スギ(Cryptomeria japonica、ヒノキChamaecyparis obtusa spp.)、クロトン(Codiaeum variegatum)、マサキ(Euonymus japonicus)、カナメモチ(Photinia glabra)等。
 芝生:シバ類(ノシバ(Zoysia japonica)、コウライシバ(Zoysia matrella)等)、バミューダグラス類(ギョウギシバ(Cynodon dactylon)等)、ベントグラス類(コヌカグサ(Agrostis gigantea)、ハイコヌカグサ(Agrostis stolonifera)、イトコヌカグサ(Agrostis capillaris)等)、ブルーグラス類(ナガハグサ(Poa pratensis)、オオスズメノカタビラ(Poa trivialis)等)、フェスク類(オニウシノケグサ(Festuca arundinacea)、イトウシノケグサ(Festuca rubra L. var. genuina)、ハイウシノケグサ(Festuca rubra L. var. commutata)等)、ライグラス類(ネズミムギ(Lolium multiflorum)、ホソムギ(Lolium perenne)等)、カモガヤ(Dactylis glomerata)、オオアワガエリ(Phleum pratense)等。
 その他:花卉類(バラ(Rosa spp.)、カーネーション(Dianthus caryophyllus)、キク(Chrysanthemum morifolium Chrysanthemum)、トルコギキョウ(Eustoma russellianum)、カスミソウ(Gypsophila spp.)、ガーベラ(Gerbera Hybrids)、マリーゴールド(Tagetes spp.)、サルビア(Salvia spp.)、ペチュニア(Petunia xhybrida)、バーベナ(Verbena spp.)、チューリップ(Tulipa gesneriana)、アスター(Callistephus chinensis)、リンドウ(Gentiana scabra var. buergeri)、ユリ(Lilium spp.)、パンジー(Viola X wittrockiana)、シクラメン(Cyclamen spp.)、ラン(Orchidaceae spp.)、スズラン(Convallaria majalis)、ラベンダー(lavender lavandula)、ストック(Matthiola incana)、ハボタン(Brassica oleracea var. acephala f. tricolor)、プリムラ(Primula spp.alt)、ポインセチア(Euphorbia pulcherrima)、グラジオラス(Gladiolus spp.)、カトレア(Cattleya spp.)、デージー(Bellis perennis)、シンビジューム(Cymbidium spp.)、ベゴニア(Begonia partita)等)、バイオ燃料植物(ヤトロファ(Jatropha curcas)、ベニバナ(Carthamus tinctorius)、アマナズナ類(Camelina sativa)、スイッチグラス(Panicum virgatum)、ミスカンサス(Miscanthus sinensis)、クサヨシ(Phalaris arundinacea))、ダンチク(Arundo donax)、ケナフ(Hibiscus cannabinus)、キャッサバ(Manihot esculenta)、ヤナギ(Salicaceae spp.)等)、観葉植物等。
Agricultural crops: corn (Zea mays), rice (Oryza sativa), wheat (Triticum aestivum), barley (Hordeum vulgare), rye (Secale cereal), oat (Avena sativa olh, sorghum) (Gossypium arboreum), soybean (Glycine max), peanut (Arachis hypogaea), buckwheat (Fagopyrum esculentum), sugar beet (Beta bulgaris ssp. Vulgaris), rape (rape. (Saccharum officinarum), tobacco (Nicotiana tabacum) and the like.
Vegetables: Eggplant (Solanum melogena), tomato (Solanum lycopersicum), green pepper (Capsicum annuum var. Angulosum), capsicum (Capsicum annuum), potato (Solanum tubulus cucumber) , Pumpkin (Cucurbita maxima), zucchini (Cucurbita pepo), watermelon (Citullus lanatas), melon (Cucumis melo) etc., cruciferous vegetables (Raphanus sativus var. , Seyo Uwasabi (Armoracia rusticana), kohlrabi (Brassica oleracea var. Gongylodes), Chinese cabbage (Brassica rapa var. Pekinensis), cabbage (Brassica Oleracea), mustard (Brassica juncea), broccoli (Brassica oleracea var. Italica), cauliflower (Brassica oleracea var Botrytis, rape (Brassica napus, etc.), asteraceae vegetables (Arcium lappa), chrysanthemum (Glebionis coronaria), artichoke (Cynara scolymus), lettuce (Lactucas lactus) ), Etc.), liliaceae vegetables (Allium fistulosum), onions (Allium cepa), garlic (Allium sativum), asparagus (Asparagus officinalis), etc., cassaceae vegetables (Daucus carrotum pelum) , Celery (Apium graveolens var. Dulc), American Bow Fu (Pastinaca sativa), etc., Red-headed vegetables (Spinacia oleracea), Chard (Veta vularis var. Cir), etc. crispa), mint (Mentha piperita), basil (Ocium basilicum, etc.), strawberry (Fragaria ananasas Duchesne), sweet potato (Ipoomoea batatas, etc.), wild potato (Dioscorea casa, Dioscorea sasa).
Fruit trees: apples (Malus pumila), Japanese pears (Pyrus communis), Japanese pears (Pyrus pyrifolia var. Culta), quince (Chaenomeles sinensis), quince (Acynomolus alo) , Plums (Prunus salicina), nectarine (Amygdalus persica var. Nectarina), ume (Prunus mume), sweet squirrel (Cerasus avium), apricot (Prunus armenia), prun (mu) , Orange (Citrus s Nensis), lemon (Citrus limon), lime (Citrus aurantifolia), grapefruit (Citrus paradisi, etc.), nuts (Castane creanas, walnut (Juglans mansura var, di) dulcis), pistachio (Pistacia vera), cashew nut (Anacardium occidentale), macadamia nutria (Macadamia integrifolia), etc., berries (Vaccinium corymbumsum, roc cranberry (on) Luck berry (Rubus fruticosus), raspberry (Rubus idaeus, etc.), grapes (Vitis lavrusca), oysters (Diospyros kaki thumberg), olives (Olea europaea), loquat (Eriobot) ), Date palmifer (Phoenix dactylifera), coconut (Cocos nucifera), oil palm (Elaeis oleifera) and the like.
Trees other than fruit trees: tea (Camellia sinensis), mulberry (Morus alba), flowering trees (Rhodendroni indicum), camellia (Camellia japonica laca), hydrangea macrophylla (Hydrangea macrophylla) Cherry (Cerasus xyedoensis), Liriodendron tulipifera, Salgersberger (Lagerstroemia indica), Kinsokusei (Osmanthus fragrance var. Aurantiacus) typhylla var.japonica), dogwood (Benthamidia florida), eucalyptus (Eucalyptus globulus), ginkgo (Ginkgo biloba), lilac (Syringa vulgaris), maple (Acer palmatum), oak (Quercus myrsinaefolia), poplar (Populus angulata), Judas tree ( Cercis chinensis, Fuyu (Liquidambar formosana), Platanus (Platanus orientalis), Japanese zelkova (Zelkova serrata), Kurobe (Thuja standishii), Japanese cypress (Abies figas) ), Juniper (Juniperus rigida), pine (Pinus densiflora), spruce (Picea jezoensis var. Hondoensis), yew (Taxus cuspidata), elm (Ulmus davidiana var. Japonica), horse chestnut (Aesculus turbinata), etc.), Sweet viburnum (Viburnum odoratissimum var. awabuki), Japanese plover (Podocarpus macrophyllus), Japanese cedar (Cryptomeria japonica, Japanese cypress Chamaecypalis obtusus spp.), Croton (Codiaeum variem sap) Photinia glabra) and the like.
Lawn: Shiba (Zoysia japonica, Zousia matrella, etc.), Bermudagrass (Cyonodon dactylon, etc.), Bentgrass (Agrostis gigante, Agrostis gigante, Agrostis gigante Agrostis capillaris, etc.), Bluegrass (Poa prairensis, Poa trivialis, etc.), Fescue (Festuka arundinacea), Fetus st. rubra L. ar. commutata), etc.), ryegrass (darnel (Lolium multiflorum), rye grass (Lolium perenne), etc.), orchard grass (Dactylis glomerata), timothy grass (Phleum pratense) and the like.
Others: Flowers (Rosa spp.), Carnation (Dianthus carylphyllus), chrysanthemum morifolium Chrysanthemum, spr. ), Salvia spp., Petunia xhybrida, Verbena spp., Tulipa gensneriana, Callistephus chinensis, Gentianarra. Lily (Lilium spp.), Pansy (Viola X wittrocciana), Cyclamen (Cyclamen spp.), Orchidaceae spp., Lily of the valley (Convallaria majlis), Lavender (lavenda laval) oleracea var. acephala f. tricolor, primula (Primula spp. alt), poinsettia (Euphorbia pulcherrima), gladiolus spp., cattleya spm. spp.), begonia (Begonia partita), etc.), biofuel plants (Jatropha curcas), safflower (Carthamus sintiva), Camelina sativus sacrum, Panicum sac. (Phalaris arundinacea)), Danchiku (Arundo donax), Kenaf (Hibiscus cannabinus), Cassava (Manihot esculenta), Willow (Salicaceae spp.), Etc.)
 前記植物の中でも、好ましい例として、ナス、トマト、ピーマン、トウガラシ、キュウリ、カボチャ、ズッキーニ、スイカ、メロン、ダイコン、カブ、ハクサイ、キャベツ、カラシナ、ブロッコリー、カリフラワー、アブラナ、ゴボウ、シュンギク、アーティチョーク、レタス、ネギ、タマネギ、ニンニク、アスパラガス、ニンジン、パセリ、セロリ、ホウレンソウ、シソ、ミント、バジル、イチゴ、サツマイモ、ヤマノイモ、サトイモを挙げることができる。 Among the plants, preferable examples include eggplant, tomato, pepper, capsicum, cucumber, pumpkin, zucchini, watermelon, melon, radish, turnip, cabbage, cabbage, mustard, broccoli, cauliflower, rape, burdock, garlic, artichoke, lettuce , Leek, onion, garlic, asparagus, carrot, parsley, celery, spinach, perilla, mint, basil, strawberry, sweet potato, yam and taro.
 上記「植物」は、遺伝子組換え技術や交配による育種法により耐性を付与された植物であってもよい。 The “plant” may be a plant to which resistance is imparted by a genetic recombination technique or a breeding method by crossing.
 本発明による誘引物質と天敵の併用は、植物又は植物の栽培地に施用することにより、植物の有害節足動物を防除するために用いられる。ここで植物としては、例えば、植物の茎葉、植物の花、植物の実、植物の種子及び植物の球根が挙げられる。なお、ここで球根とは、鱗茎、球茎、根茎、塊茎、塊根及び担根体を意味する。 The combined use of attractants and natural enemies according to the present invention is used to control harmful arthropods of plants by applying them to plants or plant cultivation areas. Here, examples of the plant include plant stems and leaves, plant flowers, plant nuts, plant seeds, and plant bulbs. In addition, a bulb means here a bulb, a bulb, a rhizome, a tuber, a tuberous root, and a root support body.
 本発明に関わる有害節足動物誘引物質と天敵の併用が防除効力を示す有害節足動物としては、例えば、次のものが挙げられる。
 外翅上目カメムシ目有害節足動物:ヒメトビウンカ(Laodelphax striatellus)、トビイロウンカ(Nilaparvata lugens)、セジロウンカ(Sogatella furcifera)等のウンカ類、ツマグロヨコバイ(Nephotettix cincticeps)、タイワンツマグロヨコバイ(Nephotettix virescens)等のヨコバイ類、ワタアブラムシ(Aphis gossypii)、モモアカアブラムシ(Myzus persicae)、ダイコンアブラムシ(Brevicoryne brassicae)、チューリップヒゲナガアブラムシ(Macrosiphum euphorbiae)、ジャガイモヒゲナガアブラムシ(Aulacorthum solani)、ムギクビレアブラムシ(Rhopalosiphum padi)、ミカンクロアブラムシ(Toxoptera citricidus)等のアブラムシ類、アオクサカメムシ(Nezara antennata)、ホソヘリカメムシ(Riptortus clavetus)、クモヘリカメムシ(Leptocorisa chinensis)、トゲシラホシカメムシ(Eysarcoris parvus)、クサギカメムシ(Halyomorpha mista)、ターニッシュッドプラントバグ(Lyus lineolaris)等のカメムシ類、オンシツコナジラミ(Trialeurodes vaporariorum)、タバココナジラミ(Bemisia tabaci)、 シルバーリーフコナジラミ(Bemisia argentifolii)等のコナジラミ類、アカマルカイガラムシ(Aonidiella aurantii)、サンホーゼカイガラムシ(Comstockaspis perniciosa)、シトラススノースケール(Unaspis citri)、ルビーロウムシ(Ceroplastes rubens)、イセリヤカイガラムシ(Icerya purchasi)等のカイガラムシ類、グンバイムシ類、キジラミ類等;
 外翅上目アザミウマ目有害節足動物:ミカンキイロアザミウマ(Frankliniella occidentais)、ミナミキイロアザミウマ(Thrips palmi)、チャノキイロアザミウマ(Scirtothrips dorsalis)、ネギアザミウマ(Thrips tabaci)、ヒラズハナアザミウマ(Frankliniella intonsa)、タバコアザミウマの(Frankliniella fusca)等のアザミウマ類等;
 内翅上目チョウ目有害節足動物:ニカメイガ(Chilo suppressalis)、サンカメイガ(Tryporyza incertulas)、コブノメイガ(Cnaphalocrocis medinalis)、ワタノメイガ(Notarcha derogata)、ノシメマダラメイガ(Plodia interpunctella)、アワノメイガ(Ostrinia furnacalis)、ヨーロピアンコーンボーラー(Ostrinia nubilaris)、ハイマダラノメイガ(Hellula undalis)、シバツトガ(Pediasia teterrellus)等のメイガ類、ハスモンヨトウ(Spodoptera litura)、シロイチモジヨトウ(Spodoptera exigua)、アワヨトウ(Pseudaletia separata)、ヨトウガ(Mamestra brassicae)、タマナヤガ(Agrotis ipsilon)、タマナギンウワバ(Plusia nigrisigna)、トリコプルシア属、ヘリオティス属、ヘリコベルパ属等のヤガ類、モンシロチョウ(Pieris rapae)等のシロチョウ類、アドキソフィエス属、ナシヒメシンクイ(Grapholita molesta)、マメシンクイガ(Leguminivora glycinivorella)、アズキサヤムシガ(Matsumuraeses azukivora)、リンゴコカクモンハマキ(Adoxophyes orana fasciata)、チャノコカクモンハマキ(Adoxophyes sp.)、チャハマキ(Homona magnanima)、ミダレカクモンハマキ(Archips fuscocupreanus)、コドリンガ(Cydia pomonella)等のハマキガ類、チャノホソガ(Caloptilia theivora)、キンモンホソガ(Phyllonorycter ringoneella)のホソガ類、モモシンクイガ(Carposina niponensis)等のシンクイガ類、リオネティア属等のハモグリガ類、リマントリア属、ユープロクティス属等のドクガ類、コナガ(Plutella xylostella)等のスガ類、ワタアカミムシ(Pectinophora gossypiella)ジャガイモガ(Phthorimaea operculella)等のキバガ類、アメリカシロヒトリ(Hyphantria cunea)等のヒトリガ類、イガ(Tinea translucens)等のヒロズコガ類等;
 内翅上目ハエ目有害節足動物:タマネギバエ(Hylemya antiqua)、タネバエ(Hylemya platura)、イネハモグリバエ(Agromyza oryzae)、イネヒメハモグリバエ(Hydrellia griseola)、イネキモグリバエ(Chlorops oryzae)、マメハモグリバエ(Liriomyza trifolii)等のハモグリバエ類、ウリミバエ(Dacus cucurbitae)、チチュウカイミバエ(Ceratitis capitata)等;
 内翅上目コウチュウ目有害節足動物:ニジュウヤホシテントウ(Epilachna vigintioctopunctata)、ウリハムシ(Aulacophora femoralis)、キスジノミハムシ(Phyllotreta striolata)、イネドロオイムシ(Oulema oryzae)、イネゾウムシ(Echinocnemus squameus)、イネミズゾウムシ(Lissorhoptrus oryzophilus)、ワタミゾウムシ(Anthonomus grandis)、アズキゾウムシ(Callosobruchus chinensis)、シバオサゾウムシ(Sphenophorus venatus)、マメコガネ(Popillia japonica)、ドウガネブイブイ(Anomala cuprea)、コーンルートワームの仲間(Diabrotica spp.)、コロラドハムシ(Leptinotarsa decemlineata)、コメツキムシの仲間(Agriotes spp.)等;
 内翅上目ハチ目有害節足動物:カブラハバチ(Athalia rosae)、ハキリアリ(Acromyrmex spp.)、ファイヤーアント(Solenopsis spp.)等;
 多新翅上目バッタ目有害節足動物:ケラ(Gryllotalpa africana)、コバネイナゴ(Oxya yezoensis)、ハネナガイナゴ(Oxya japonica)等;
 ダニ目有害節足動物:ナミハダニ(Tetranychus urticae)、ミカンハダニ(Panonychus citri)、オリゴニカス属等のハダニ類、ミカンサビダニ(Aculops pelekassi)等のフシダニ類、チャノホコリダニ(Polyphagotarsonemus latus)等のホコリダニ類、ヒメハダニ類、ケナガハダニ類、ケナガコナダニ(Tyrophagus putrescentiae)等のコナダニ類、コナヒョウヒダニ(Dermatophagoides farinae)、ヤケヒョウヒダニ(Dermatophagoides ptrenyssnus)等のヒョウヒダニ類、ホソツメダニ(Cheyletus eruditus)、クワガタツメダニ(Cheyletus malaccensis)、ミナミツメダニ(Cheyletus moorei)等のツメダニ類等;
 線虫類:イネシンガレセンチュウ(Aphelenchoides besseyi)、イチゴメセンチュウ(Nothotylenchus acris)等。
Examples of harmful arthropods for which the combined use of a harmful arthropod attractant and natural enemies according to the present invention exhibit a controlling effect include the following.
Species of the outer pods, Pteridopsis, Arthropoda: Lalodelphax striatellas, Japanese planthoppers (Nilaparvata lugens, etc.), Japanese planthoppers (Sugatella furcifera), etc. Cotton aphids (Aphis gossypii), peach aphids (Myzus persicae), radish aphids (Brevicoryne brassicae), tulip beetle aphids (Macrosiphum euphorbiae), potato aphid ni), wheat aphids (Rhopalosiphum padi), aphids such as the citrus aphid (Toxoptera citriticidus), Nezara antenatum (Shirasotemushishito), Riptortus (Liptortus) (Eysarcoris parvus), smelt beetle (Halyomorpha mista), stink bugs such as the finished plant bug (Lyus lineolaris), Trialureodes vapariorium, B. white flies such as ia argentifolii, Aonidiella aurantiii, Santoschai scales (Comstockcaspis perniciosa), citrus snow scale (Unaspis citrus), rubirium beetles , Whales, etc .;
Eccentric arthropods of the outer limbs: Franklinella occidentais, Trips palmi, Falcones, Scitotrips dorsalis Thrips such as (Franklinella fusca), etc .;
Pleuropoda: Arthropoda: Chilo suppressalis, Trypolyza incertulas, Canaphalocis medinais, Ota, Pepper, Pepperum Corn borers (Ostrinia nubilaris), Hydra undamaris (Hellula undalis), Shibatatsuga (Pediasia tererrellus), etc., Spodoptera litura, Sirochimodoitopo Pseudoaletia separa, Pseudomonas genus, Heliotis genus, Heliotis genus, Heliostis genus , Nashihimeshinsukui (Graphophrita molesta), Beansinkigaiga (Leguminivora glycinnivorella), Azukiyamamushiga (Matsumuraeses azukivivora), Apple Kokumonmona (Adoxophora) Chahamaki (Homona magnanima), Mi someone summer fruit moth (Archips fuscocupreanus), Tortricidae such as codling moth (Cydia pomonella), Chanohosoga (Caloptilia theivora), subfraction class, Shinkuiga such as peach fruit moth (Carposina niponensis) of the apple leaf miner (Phyllonorycter ringoneella), Anemone species such as Rionetia spp., Doctors such as Limantria spp., Euprocutis spp., Sugas such as Plutella xylostella, Peptinophora gossypiella potato moths (Phtholimaea opera spp. Arctiidae such as Kashirohitori (Hyphantria cunea), clothes moth (Tinea translucens) Hirozukoga such as such;
Species of Arthropoda: Hemimya antiqua, Hemimya platya, Amymyza oryzae roe, Ms. And the like, the leaf fly (Dacus cucurbitae), the fruit fly (Ceratitis capitata) and the like;
Inner 翅上 eyes Coleoptera arthropod pests: beetle, Epilachna vigintioctopunctata (Epilachna vigintioctopunctata), cucurbit leaf beetle (Aulacophora femoralis), Kisujinomihamushi (Phyllotreta striolata), Inedorooimushi (Oulema oryzae), rice weevil (Echinocnemus squameus), rice water weevil (Lissorhoptrus oryzophilus), Cotton weevil (Anthonomus grandis), Azuki beetle (Callosobrchuchus chinensis), Shibao weevil (Sphenophorus venatus), Japanese beetle (Popilia japonica), Doganebuibu omala cuprea, corn root worm mate (Diabrotica spp.), Colorado potato beetle (Leptinotarsa decmlineata), click beetle mate (Agriotes spp.), etc .;
Inner lepidopterous wasp harmful arthropods: Atharia rosae, Achillyrmex spp., Fire Ant (Solenopsis spp.), Etc .;
Pleuromorpha: Grasshopper harmful arthropods: Kelly (Grylotalpa africana), Oxana yezoensis, Oxya japonica, etc .;
Tickless arthropods: Tetranychus urticae, Pannychus citri, Oticidae genus mite, Acarops pelekassi, etc., Mite mite (Polyphagous mite, Polyphagous mite) Dust mites, Tyrophagus putrescentiae and other mites, Dermatophagoides farinae, Dustophaeces tick alaccensis), Minami Tsumedani (Cheyletus moorei) Tsumedani such as the like;
C. elegans: rice scentless nematode (Aphelenchodes besseyi), strawberry nematode (Notothylenchus acris) and the like.
 本発明に関わる有害節足動物誘引物質および天敵が防除効力を示す有害節足動物としては、好ましくは外翅上目カメムシ目有害節足動物、外翅上目アザミウマ目有害節足動物、内翅上目ハエ目有害節足動物、ダニ目有害節足動物、より好ましくは外翅上目アザミウマ目有害節足動物が挙げられる。 The harmful arthropod-inducing substance and natural enemies related to the present invention are preferably harmful arthropods that are effective in controlling the outer enemies. Examples include upper-fly fly harmful arthropods, tick-eye harmful arthropods, and more preferably outer limbs, thrips-eye harmful arthropods.
 以下、本発明をその効果等を示す試験例とともにさらに具体的に説明するが、これらに本発明の技術的範囲を限定することを意図するものではない。 Hereinafter, the present invention will be described more specifically together with test examples showing its effects and the like, but it is not intended to limit the technical scope of the present invention thereto.
試験例1 誘引剤、または天敵を個別に処理した場合の誘引効果
 p-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、ゲラニオール、リナロール、ネロール、シトロネロール、o-アニスアルデヒド、β-ファルネセン、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、オイゲノール、3-フェニルプロピルアルデヒド、シンナムアルデヒド、ユーカリプトール、スクアレン、α-ヘキシルシンナムアルデヒドから選択された各単剤1μgの重量を含むヘキサン溶液を調整した。この溶液を濾紙に含浸させた後、プラスチック製のアイスクリームカップ内に設置された着色粘着板中央部に貼付した。その後、アイスクリームカップ内に20頭のミカンキイロアザミウマ雌成虫を放飼すると共に、アザミウマの餌として、水を含んだ脱脂綿を敷いたインゲン葉片を設置した。24時間後、着色粘着トラップに捕獲されたアザミウマ数を計測し、式1を用い誘引率を算出した。その結果を表1に示す。
 一方、上記の誘引単剤処理とは別に、アイスクリームカップ内に着色粘着板を設置した。そのアイスクリームカップ内に2頭のスワルスキーカブリダニ成虫を放飼した。その後、アイスクリームカップ内に20頭のミカンキイロアザミウマ雌成虫を放飼すると共に、アザミウマの餌として、水を含んだ脱脂綿を敷いたインゲン葉片を設置した。24時間後、着色粘着トラップに捕獲されたアザミウマ数を計測し、式1を用い誘引率を算出した。その結果を表1に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
Figure JPOXMLDOC01-appb-T000001
Test Example 1 Attracting effect when individual attractants or natural enemies are treated p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, β-farnesene, methyl anthranilate, benzoic acid Selected from methyl acetate, o-aminoacetophenone, o-anisidine, methyl m-aminobenzoate, methyl o-toluate, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene, α-hexylcinnamaldehyde A hexane solution containing 1 μg of each single agent was prepared. After this solution was impregnated into filter paper, it was attached to the central part of the colored adhesive plate installed in a plastic ice cream cup. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for the thrips. After 24 hours, the number of thrips captured in the colored adhesive trap was counted, and the attraction rate was calculated using Equation 1. The results are shown in Table 1.
On the other hand, a colored pressure-sensitive adhesive plate was installed in the ice cream cup separately from the above attracting single agent treatment. In the ice cream cup, two adult Swarski Kaburani mites were released. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for the thrips. After 24 hours, the number of thrips captured in the colored adhesive trap was counted, and the attraction rate was calculated using Equation 1. The results are shown in Table 1.
“Formula 1”: Attracting rate = (Number of catches in the treated area−Number of catches in the untreated area) * 100 / (Total number of released insects per cup)
Figure JPOXMLDOC01-appb-T000001
試験例2 誘引剤と天敵の相乗効果
 p-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、ゲラニオール、リナロール、ネロール、シトロネロール、o-アニスアルデヒド、β-ファルネセン、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、オイゲノール、3-フェニルプロピルアルデヒド、シンナムアルデヒド、ユーカリプトール、スクアレン、α-ヘキシルシンナムアルデヒドから選択された各単剤1μgの重量を含むヘキサン溶液を調整した。この溶液を濾紙に含浸させた後、プラスチック製のアイスクリームカップ内に設置された着色粘着板中央部に貼付した。同時に、アイスクリームカップ内に2頭のスワルスキーカブリダニ成虫を放飼した。その後、アイスクリームカップ内に20頭のミカンキイロアザミウマ雌成虫を放飼すると共に、アザミウマの餌として、水を含んだ脱脂綿を敷いたインゲン葉片を設置した。24時間後、着色粘着トラップに捕獲されたアザミウマ数を計測し、式1を用い誘引率を算出した。その結果を表2に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
一般に、与えられた1種類の有効成分化合物および1種類の天敵を同時処理した際に期待される誘引相乗効果は下記の式2のコルビーの計算式により求められる。
「式2」;E=X+Y-(X*Y)/100
X:有効成分化合物Aを処理したときの誘引率
Y:天敵Bを処理したときの誘引率
E:有効成分化合物Aおよび天敵Bを同時に処理したときに期待される誘引率(誘引率期待値)
Figure JPOXMLDOC01-appb-T000002
Test Example 2 Synergistic effect of attractant and natural enemy p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, β-farnesene, methyl anthranilate, methyl benzoate, o-aminoacetophenone Weight of 1 μg of each single agent selected from o-anisidine, methyl m-aminobenzoate, methyl o-toluylate, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene, α-hexylcinnamaldehyde A hexane solution containing was prepared. After this solution was impregnated into filter paper, it was attached to the central part of the colored adhesive plate installed in a plastic ice cream cup. At the same time, two adult Swarski Kaburani mites were released in an ice cream cup. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for the thrips. After 24 hours, the number of thrips captured in the colored adhesive trap was counted, and the attraction rate was calculated using Equation 1. The results are shown in Table 2.
“Formula 1”: Attracting rate = (Number of catches in treated area−number of catches in untreated area) * 100 / (total number of released insects per cup)
In general, the attraction synergistic effect expected when a given active ingredient compound and a natural enemy are simultaneously treated can be obtained from the following Colby calculation formula.
“Formula 2”; E = X + Y− (X * Y) / 100
X: Attraction rate when treating active ingredient compound A Y: Attraction rate when treating natural enemy B E: Attraction rate expected when active ingredient compound A and natural enemy B are treated simultaneously (expected attracting value)
Figure JPOXMLDOC01-appb-T000002
試験例3 誘引剤、または捕食性天敵を個別に処理した場合の誘引効果
 p-アニスアルデヒド、ネロール、スクアレンから選択された各単剤1μgの重量を含むヘキサン溶液を調整し、この溶液を濾紙に含浸させた後、プラスチック製のアイスクリームカップ内に設置された着色粘着板中央部に上記濾紙を貼付した。その後、アイスクリームカップ内に20頭のミカンキイロアザミウマ雌成虫を放飼すると共に、アザミウマの餌として、水を含んだ脱脂綿を敷いたインゲン葉片を設置した。24時間後、着色粘着トラップに捕獲されたミカンキイロアザミウマ数を計測し、式1を用い誘引率を算出した。その結果を表3に示す。
 一方、上記の誘引単剤処理とは別に、同様の着色粘着板を設置したアイスクリームカップ内に2頭のタイリクヒメハナカメムシ成虫を放飼した。その後、アイスクリームカップ内に20頭のミカンキイロアザミウマ雌成虫を放飼すると共に、アザミウマの餌として、水を含んだ脱脂綿を敷いたインゲン葉片を設置した。24時間後、着色粘着トラップに捕獲されたミカンキイロアザミウマ数を計測し、式1を用い誘引率を算出した。その結果を表3に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
Figure JPOXMLDOC01-appb-T000003
Test Example 3 Attracting effect when individual attractants or predatory natural enemies are treated individually A hexane solution containing 1 μg of each single agent selected from p-anisaldehyde, nerol and squalene is prepared, and this solution is applied to filter paper. After the impregnation, the filter paper was attached to the central part of the colored adhesive plate installed in the plastic ice cream cup. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for the thrips. After 24 hours, the number of orange thrips captured in the colored sticky trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 3.
On the other hand, apart from the above attracting single agent treatment, two adults of the mosquito bug were released in an ice cream cup provided with the same colored adhesive plate. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for the thrips. After 24 hours, the number of orange thrips captured in the colored sticky trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 3.
“Formula 1”: Attracting rate = (Number of catches in the treated area−Number of catches in the untreated area) * 100 / (Total number of released insects per cup)
Figure JPOXMLDOC01-appb-T000003
試験例4 誘引剤と捕食性天敵の相乗効果
 p-アニスアルデヒド、ネロール、スクアレンから選択された各単剤1μgの重量を含むヘキサン溶液を調整した。この溶液を濾紙に含浸させた後、プラスチック製のアイスクリームカップ内に設置された着色粘着板中央部に貼付した。同時に、アイスクリームカップ内に2頭のタイリクヒメハナカメムシ成虫を放飼した。その後、アイスクリームカップ内に20頭のミカンキイロアザミウマ雌成虫を放飼すると共に、ミカンキイロアザミウマの餌として、水を含んだ脱脂綿を敷いたインゲン葉片を設置した。24時間後、着色粘着トラップに捕獲されたミカンキイロアザミウマ数を計測し、式1を用い誘引率を算出した。その結果を表4に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
一般に、与えられた1種類の有効成分化合物および1種類の天敵を同時処理した際に期待される誘引相乗効果は下記の式2のコルビーの計算式により求められる。
「式2」;E=X+Y-(X*Y)/100
X:有効成分化合物Aを処理したときの誘引率
Y:天敵Bを処理したときの誘引率
E:有効成分化合物Aおよび天敵Bを同時に処理したときに期待される誘引率(誘引率期待値)
Figure JPOXMLDOC01-appb-T000004
Test Example 4 Synergistic effect of attractant and predatory natural enemy A hexane solution containing 1 μg of each single agent selected from p-anisaldehyde, nerol and squalene was prepared. After this solution was impregnated into filter paper, it was attached to the central part of the colored adhesive plate installed in a plastic ice cream cup. At the same time, two adults of the mosquito bug were released in an ice cream cup. Thereafter, 20 adult females of Thrips thrips were released in an ice cream cup, and green leaf pieces laid with absorbent cotton containing water were placed as food for the Thrips thrips. After 24 hours, the number of orange thrips captured in the colored sticky trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 4.
“Formula 1”: Attracting rate = (Number of catches in the treated area−Number of catches in the untreated area) * 100 / (Total number of released insects per cup)
In general, the attraction synergistic effect expected when a given active ingredient compound and a natural enemy are simultaneously treated can be obtained from the following Colby calculation formula.
“Formula 2”; E = X + Y− (X * Y) / 100
X: Attraction rate when treating active ingredient compound A Y: Attraction rate when treating natural enemy B E: Attraction rate expected when active ingredient compound A and natural enemy B are treated simultaneously (expected attracting value)
Figure JPOXMLDOC01-appb-T000004
試験例5 誘引剤、または捕食性天敵を個別に処理した場合の誘引効果
 p-アニスアルデヒド、リナロール、シトロネロール、o-アニスアルデヒド、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-トルイル酸メチル、オイゲノール、シンナムアルデヒド、ユーカリプトール、スクアレン、α-ヘキシルシンナムアルデヒドから選択された各単剤1μgの重量を含むヘキサン溶液を調整し、この溶液を濾紙に含浸させた後、プラスチック製のアイスクリームカップ内に設置された着色粘着板中央部に上記濾紙を貼付した。その後、アイスクリームカップ内に20頭のネギアザミウマ雌成虫を放飼すると共に、アザミウマの餌として、水を含んだ脱脂綿を敷いたインゲン葉片を設置した。24時間後、着色粘着トラップに捕獲されたネギアザミウマ数を計測し、式1を用い誘引率を算出した。その結果を表5に示す。
 一方、上記の誘引単剤処理とは別に、同様の着色粘着板を設置したアイスクリームカップ内に2頭のタイリクヒメハナカメムシ成虫を放飼した。その後、アイスクリームカップ内に20頭のネギアザミウマ雌成虫を放飼すると共に、アザミウマの餌として、水を含んだ脱脂綿を敷いたインゲン葉片を設置した。24時間後、着色粘着トラップに捕獲されたネギアザミウマ数を計測し、式1を用い誘引率を算出した。その結果を表5に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
Figure JPOXMLDOC01-appb-T000005
Test Example 5 Attracting effect when individual attractants or predatory natural enemies are treated p-anisaldehyde, linalool, citronellol, o-anisaldehyde, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-toluic acid A hexane solution containing 1 μg of each single agent selected from methyl, eugenol, cinnamaldehyde, eucalyptol, squalene, and α-hexylcinnamaldehyde was prepared, and this solution was impregnated into filter paper. The filter paper was affixed to the central part of the colored adhesive plate installed in the cream cup. After that, 20 adult females of Thrips thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for thrips. After 24 hours, the number of Negia thrips captured in the colored adhesive trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 5.
On the other hand, apart from the above attracting single agent treatment, two adults of the mosquito bug were released in an ice cream cup provided with the same colored adhesive plate. After that, 20 adult females of Thrips thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for thrips. After 24 hours, the number of Negia thrips captured in the colored adhesive trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 5.
“Formula 1”: Attracting rate = (Number of catches in the treated area−Number of catches in the untreated area) * 100 / (Total number of released insects per cup)
Figure JPOXMLDOC01-appb-T000005
試験例6 誘引剤と捕食性天敵の相乗効果
 p-アニスアルデヒド、リナロール、シトロネロール、o-アニスアルデヒド、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-トルイル酸メチル、オイゲノール、シンナムアルデヒド、ユーカリプトール、スクアレン、α-ヘキシルシンナムアルデヒドから選択された各単剤1μgの重量を含むヘキサン溶液を調整した。この溶液を濾紙に含浸させた後、プラスチック製のアイスクリームカップ内に設置された着色粘着板中央部に貼付した。同時に、アイスクリームカップ内に2頭のタイリクヒメハナカメムシ成虫を放飼した。その後、アイスクリームカップ内に20頭のネギアザミウマ雌成虫を放飼すると共に、ネギアザミウマの餌として、水を含んだ脱脂綿を敷いたインゲン葉片を設置した。24時間後、着色粘着トラップに捕獲されたネギアザミウマ数を計測し、式1を用い誘引率を算出した。その結果を表6に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
一般に、与えられた1種類の有効成分化合物および1種類の天敵を同時処理した際に期待される誘引相乗効果は下記の式2のコルビーの計算式により求められる。
「式2」;E=X+Y-(X*Y)/100
X:有効成分化合物Aを処理したときの誘引率
Y:天敵Bを処理したときの誘引率
E:有効成分化合物Aおよび天敵Bを同時に処理したときに期待される誘引率(誘引率期待値)
Figure JPOXMLDOC01-appb-T000006
Test Example 6 Synergistic effect of attractant and predatory natural enemy p-anisaldehyde, linalool, citronellol, o-anisaldehyde, methyl anthranilate, methyl benzoate, o-aminoacetophenone, methyl o-toluate, eugenol, cinnamaldehyde, A hexane solution containing 1 μg of each single agent selected from eucalyptol, squalene, and α-hexylcinnamaldehyde was prepared. After this solution was impregnated into filter paper, it was attached to the central part of the colored adhesive plate installed in a plastic ice cream cup. At the same time, two adults of the mosquito bug were released in an ice cream cup. Thereafter, 20 adult females of Nigeria thrips were released in an ice cream cup, and green beans with water-absorbent cotton were placed as food for the Nigeria thrips. After 24 hours, the number of Negia thrips captured in the colored adhesive trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 6.
“Formula 1”: Attracting rate = (Number of catches in the treated area−Number of catches in the untreated area) * 100 / (Total number of released insects per cup)
In general, the attraction synergistic effect expected when a given active ingredient compound and a natural enemy are simultaneously treated can be obtained from the following Colby calculation formula.
“Formula 2”; E = X + Y− (X * Y) / 100
X: Attraction rate when treating active ingredient compound A Y: Attraction rate when treating natural enemy B E: Attraction rate expected when active ingredient compound A and natural enemy B are treated simultaneously (expected attracting value)
Figure JPOXMLDOC01-appb-T000006
試験例7 誘引剤、または捕食性天敵を個別に処理した場合の誘引効果
 o-アニスアルデヒド、安息香酸メチル、o-アミノアセトフェノンから選択された各単剤1μgの重量を含むヘキサン溶液を調整し、この溶液を濾紙に含浸させた。プラスチック製のアイスクリームカップ内に水を含んだ脱脂綿を敷き、この上に着色粘着板と、ナミハダニの餌としてインゲン葉片を重ねて設置して、粘着板中央部に誘引化合物を含んだ上記濾紙を貼付した。その後、アイスクリームカップ内に15頭のナミハダニを放飼した。24時間後、着色粘着トラップに捕獲されたハダニ数を計測し、式1を用い誘引率を算出した。その結果を表7に示す。
 一方、上記の誘引単剤処理とは別に、水を含んだ脱脂綿上に着色粘着板及びインゲン葉片を設置した同様のアイスクリームカップ内に2頭のチリカブリダニ成虫を放飼し、その後、アイスクリームカップ内に15頭のナミハダニを放飼した。24時間後、着色粘着トラップに捕獲されたナミハダニ数を計測し、式1を用い誘引率を算出した。その結果を表7に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
Figure JPOXMLDOC01-appb-T000007
Test Example 7 Attracting effect when individually treated with attractant or predatory natural enemy Prepare a hexane solution containing 1 μg of each single agent selected from o-anisaldehyde, methyl benzoate and o-aminoacetophenone, This solution was impregnated into filter paper. Place the absorbent cotton containing water in a plastic ice cream cup, place a colored sticky plate on top of this and the green leaf pieces as food for the spider mite, and place the filter paper containing the attracting compound in the center of the sticky plate Affixed. Thereafter, 15 nymph mites were released in the ice cream cup. After 24 hours, the number of spider mites captured in the colored adhesive trap was counted, and the attraction rate was calculated using Equation 1. The results are shown in Table 7.
On the other hand, apart from the above attracting single agent treatment, two adult dust mite mites were released in a similar ice cream cup in which a colored sticky plate and kidney beans were placed on absorbent cotton containing water, and then an ice cream cup Inside, 15 nymphs were released. After 24 hours, the number of spider mites captured in the colored sticky trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 7.
“Formula 1”: Attracting rate = (Number of catches in the treated area−Number of catches in the untreated area) * 100 / (Total number of released insects per cup)
Figure JPOXMLDOC01-appb-T000007
試験例8 誘引剤と捕食性天敵の相乗効果
 o-アニスアルデヒド、安息香酸メチル、o-アミノアセトフェノンから選択された各単剤1μgの重量を含むヘキサン溶液を調整し、この溶液を濾紙に含浸させた。プラスチック製のアイスクリームカップ内に水を含んだ脱脂綿を敷き、この上に着色粘着板と、ナミハダニの餌としてインゲン葉片を重ねて設置して、粘着板中央部に誘引化合物を含んだ上記濾紙を貼付した。同時に、アイスクリームカップ内に2頭のチリカブリダニ成虫を放飼し、その後、アイスクリームカップ内に15頭のナミハダニを放飼した。24時間後、着色粘着トラップに捕獲されたハダニ数を計測し、式1を用い誘引率を算出した。その結果を表8に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
一般に、与えられた1種類の有効成分化合物および1種類の天敵を同時処理した際に期待される誘引相乗効果は下記の式2のコルビーの計算式により求められる。
「式2」;E=X+Y-(X*Y)/100
X:有効成分化合物Aを処理したときの誘引率
Y:天敵Bを処理したときの誘引率
E:有効成分化合物Aおよび天敵Bを同時に処理したときに期待される誘引率(誘引率期待値)
Figure JPOXMLDOC01-appb-T000008
Test Example 8 Synergistic effect of attractant and predatory natural enemy A hexane solution containing 1 μg of each single agent selected from o-anisaldehyde, methyl benzoate and o-aminoacetophenone was prepared, and this solution was impregnated on filter paper. It was. Place the absorbent cotton containing water in a plastic ice cream cup, place a colored sticky plate on top of this and the green leaf pieces as food for the spider mite, and place the filter paper containing the attracting compound in the center of the sticky plate Affixed. At the same time, two adult dust mites were released in the ice cream cup, and then 15 nymph mites were released in the ice cream cup. After 24 hours, the number of spider mites captured in the colored adhesive trap was counted, and the attraction rate was calculated using Equation 1. The results are shown in Table 8.
“Formula 1”: Attracting rate = (Number of catches in the treated area−Number of catches in the untreated area) * 100 / (Total number of released insects per cup)
In general, the attraction synergistic effect expected when a given active ingredient compound and a natural enemy are simultaneously treated can be obtained from the following Colby calculation formula.
“Formula 2”; E = X + Y− (X * Y) / 100
X: Attraction rate when treating active ingredient compound A Y: Attraction rate when treating natural enemy B E: Attraction rate expected when active ingredient compound A and natural enemy B are treated simultaneously (expected attracting value)
Figure JPOXMLDOC01-appb-T000008
試験例9 誘引剤、または寄生性天敵を個別に処理した場合の誘引効果
 ニコチン酸エチル、リナロール、シトロネロール、安息香酸メチル、シンナムアルデヒドから選択された各単剤1μgの重量を含むヘキサン溶液を調整し、この溶液を濾紙に含浸させた。プラスチック製のアイスクリームカップ内に水を含んだ脱脂綿を敷き、この上に着色粘着板と、アブラムシの餌としてインゲン葉片を重ねて設置して、粘着板中央部に誘引化合物を含んだ誘引化合物を含んだ上記濾紙を貼付した。その後、アイスクリームカップ内に15頭のモモアカアブラムシを放飼した。24時間後、着色粘着トラップに捕獲されたアブラムシ数を計測し、式1を用い誘引率を算出した。その結果を表9に示す。
 一方、上記の誘引単剤処理とは別に、水を含んだ脱脂綿上に着色粘着板及びインゲン葉片を設置した同様のアイスクリームカップ内に2頭のコレマンアブラバチ成虫を放飼し、その後、アイスクリームカップ内に15頭のモモアカアブラムシを放飼した。24時間後、着色粘着トラップに捕獲されたアブラムシ数を計測し、式1を用い誘引率を算出した。その結果を表9に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
Figure JPOXMLDOC01-appb-T000009
Test Example 9 Attracting effect when individual attractants or parasitic natural enemies are treated individually A hexane solution containing 1 μg of each single agent selected from ethyl nicotinate, linalool, citronellol, methyl benzoate and cinnamaldehyde was prepared. The filter paper was impregnated with this solution. Place an absorbent cotton containing water in a plastic ice cream cup, place a colored sticky plate on top of this and a green leaf leaf as a aphid bait, and place an attracting compound containing an attracting compound in the center of the sticky plate The filter paper contained was affixed. Thereafter, 15 peach aphids were released in the ice cream cup. After 24 hours, the number of aphids captured in the colored adhesive trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 9.
On the other hand, apart from the above attracting single agent treatment, two Coleman wasp adults were released in a similar ice cream cup in which a colored sticky plate and kidney beans were placed on absorbent cotton containing water. Fifteen peach aphids were released in the cream cup. After 24 hours, the number of aphids captured in the colored adhesive trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 9.
“Formula 1”: Attracting rate = (Number of catches in the treated area−Number of catches in the untreated area) * 100 / (Total number of released insects per cup)
Figure JPOXMLDOC01-appb-T000009
試験例10 誘引剤と寄生性天敵の相乗効果
 ニコチン酸エチル、リナロール、シトロネロール、安息香酸メチル、シンナムアルデヒドから選択された各単剤1μgの重量を含むヘキサン溶液を調整し、この溶液を濾紙に含浸させた。プラスチック製のアイスクリームカップ内に水を含んだ脱脂綿を敷き、この上に着色粘着板と、アブラムシの餌としてインゲン葉片を重ねて設置して、粘着板中央部に誘引化合物を含んだ上記濾紙を貼付した。同時に、アイスクリームカップ内に2頭のコレマンアブラバチ成虫を放飼し、その後、アイスクリームカップ内に15頭のモモアカアブラムシを放飼した。24時間後、着色粘着トラップに捕獲されたアブラムシ数を計測し、式1を用い誘引率を算出した。その結果を表10に示す。
「式1」;誘引率=(処理区の捕獲数-無処理区の捕獲数)*100/(1カップあたりの総放飼虫数)
一般に、与えられた1種類の有効成分化合物および1種類の天敵を同時処理した際に期待される誘引相乗効果は下記の式2のコルビーの計算式により求められる。
「式2」;E=X+Y-(X*Y)/100
X:有効成分化合物Aを処理したときの誘引率
Y:天敵Bを処理したときの誘引率
E:有効成分化合物Aおよび天敵Bを同時に処理したときに期待される誘引率(誘引率期待値)
Figure JPOXMLDOC01-appb-T000010
Test Example 10 Synergistic effect of attractant and parasitic natural enemy Prepare a hexane solution containing 1 μg of each single agent selected from ethyl nicotinate, linalool, citronellol, methyl benzoate, and cinnamaldehyde, and impregnate the filter paper with this solution. I let you. Place the absorbent cotton containing water in a plastic ice cream cup, place a colored sticky board on top of this and a green leaf piece as food for aphids, and place the filter paper containing the attracting compound in the center of the sticky board. Affixed. At the same time, two adult Koreman Aboubati were released in the ice cream cup, and then 15 peach aphids were released in the ice cream cup. After 24 hours, the number of aphids captured in the colored adhesive trap was measured, and the attraction rate was calculated using Equation 1. The results are shown in Table 10.
“Formula 1”: Attracting rate = (Number of catches in treated area−number of catches in untreated area) * 100 / (total number of released insects per cup)
In general, the attraction synergistic effect expected when a given active ingredient compound and a natural enemy are simultaneously treated can be obtained from the following Colby calculation formula.
“Formula 2”; E = X + Y− (X * Y) / 100
X: Attraction rate when treating active ingredient compound A Y: Attraction rate when treating natural enemy B E: Attraction rate expected when active ingredient compound A and natural enemy B are treated simultaneously (expected attracting value)
Figure JPOXMLDOC01-appb-T000010
試験例11 誘引化合物、または天敵微生物を個別に処理した場合の誘引効果
 p-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、ゲラニオール、リナロール、ネロール、シトロネロール、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、α-ヘキシルシンナムアルデヒド、o-アニスアルデヒド、β‐ファルネセン、オイゲノール、3‐フェニルプロピルアルデヒド、シンナムアルデヒド、ユーカリプトール、スクアレンから選択された各単一誘引化合物1μgの重量を含むヘキサン溶液を調整した。この溶液を濾紙に含浸させた後、ガラス管の片端に設置した。加えて、アザミウマの餌として、一定面積(1cm)に切断したインゲン葉片を両端に設置した。このとき、誘引化合物を設置した端を処理区とした。ガラス管の片側から1管につき20個体のミカンキイロアザミウマ雌成虫を管中央付近まで挿入し、管の両端にスポンジの栓をした。試験開始から30分後にアザミウマが処理区に定位した場合を、誘引されたものと定義した。同様の試験を複数回行い、誘引化合物に誘引された個体数を基に、式1を用いて誘引化合物への誘引率を算出した。その結果を表11に表す。
 一方、上記の単一の誘引化合物処理とは別に、現在一般的に使用されている天敵微生物から選択された、各実用濃度に希釈した各単一天敵微生物の溶液にインゲン葉を浸漬し風乾した後、上記試験のインゲン葉片と同面積に切断した。ガラス管の片側に天敵微生物を処理したインゲン葉片を、もう一方に無処理のインゲン葉片を設置し、上記試験と同様に、ミカンキイロアザミウマを中央付近に挿入し、栓をした。試験開始から30分後、天敵微生物を処理していない無処理葉片に誘引された個体数を基に、無処理葉片への誘引率(天敵微生物処理葉片への忌避率)を算出した。その結果を表11に示す。
「式1」:誘引化合物への誘引率=(処理区に誘引された個体数-コントロールに誘引された個体数)*100/(試験数)
無処理葉片への誘引率(天敵微生物処理葉片への忌避率)=(無処理葉片に誘引された個体数-コントロールに誘引された個体数)*100/(試験数)
式中の「コントロールに誘引された」とは、誘引化合物・天敵微生物のいずれも処理していない葉片をガラス管の両端に設置した場合の、片側の葉片に誘引されることを示す。
Figure JPOXMLDOC01-appb-T000011
Test Example 11 Attracting effect when individual attracting compounds or natural enemy microorganisms were treated p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, methyl anthranilate, methyl benzoate, o-aminoacetophenone, Selected from o-anisidine, methyl m-aminobenzoate, methyl o-toluate, α-hexylcinnamaldehyde, o-anisaldehyde, β-farnesene, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene A hexane solution containing 1 μg of each single attractant compound was prepared. After this solution was impregnated into filter paper, it was placed on one end of a glass tube. In addition, kidney beans cut into a certain area (1 cm 2 ) were placed at both ends as food for thrips. At this time, the end where the attracting compound was installed was defined as a treatment zone. From one side of the glass tube, 20 adult female thrips of thrips were inserted to the vicinity of the center of the tube, and sponge plugs were plugged at both ends of the tube. The case where thrips were localized in the treatment area 30 minutes after the start of the test was defined as attracted. A similar test was performed a plurality of times, and the attraction rate to the attracting compound was calculated using Equation 1 based on the number of individuals attracted by the attracting compound. The results are shown in Table 11.
On the other hand, separately from the above-mentioned single attracting compound treatment, kidney beans were immersed in a solution of each natural enemy microorganism selected from currently commonly used natural enemy microorganisms diluted to each practical concentration and air-dried. Then, it cut | disconnected in the same area as the bean leaf piece of the said test. A bean leaf piece treated with natural enemy microorganisms was placed on one side of a glass tube, and an untreated bean leaf piece was placed on the other side. Similarly to the above-described test, the orange thrips were inserted near the center and plugged. 30 minutes after the start of the test, the attraction rate to the untreated leaf pieces (repellency to the natural enemy microorganism treated leaf pieces) was calculated based on the number of individuals attracted to the untreated leaf pieces not treated with the natural enemy microorganisms. The results are shown in Table 11.
“Formula 1”: Attracting ratio to attracting compound = (number of individuals attracted to treatment area−number of individuals attracted to control) * 100 / (number of tests)
Attraction rate to untreated leaf pieces (repellency to natural enemy microorganism treated leaf pieces) = (number of individuals attracted to untreated leaf pieces-number of individuals attracted to control) * 100 / (number of tests)
“Attracted by control” in the formula indicates that the leaf pieces not treated with either the attracting compound or the natural enemy microorganisms are attracted to the leaf pieces on one side when they are placed at both ends of the glass tube.
Figure JPOXMLDOC01-appb-T000011
試験例12 誘引化合物と天敵微生物の相乗効果
 p-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、ゲラニオール、リナロール、ネロール、シトロネロール、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、α-ヘキシルシンナムアルデヒド、o-アニスアルデヒド、β‐ファルネセン、オイゲノール、3‐フェニルプロピルアルデヒド、シンナムアルデヒド、ユーカリプトール、スクアレンから選択された各単一誘引化合物1μgの重量を含むヘキサン溶液を調整した。この溶液を濾紙に含浸させた後、ガラス管内の片端に設置し、加えて、アザミウマの餌として、一定面積(1cm)に切断したインゲン葉片を同じ側の管内端に設置した。
 一方、現在一般的に使用されている天敵微生物から選択された、各実用濃度に希釈した各単一天敵微生物の溶液にインゲン葉を浸漬し風乾した後、上記インゲン葉片と同じ面積に切断し、誘引化合物が無処理の、もう一方のガラス管内端に設置した。このとき、誘引化合物を設置した端を処理区とした。その後、ガラス管の片側から1管につき20個体のミカンキイロアザミウマ雌成虫を管中央付近まで挿入し、管の両端にスポンジの栓をした。試験開始30分後にアザミウマが処理区に定位した場合を誘引されたものと定義した。同様の試験を複数回行い、誘引化合物に誘引された個体数を基に、式1を用いて誘引化合物への誘引率を算出した。その結果を表12に表す。
「式1」:誘引化合物への誘引率=(処理区に誘引された個体数-コントロールに誘引された個体数)*100/(試験数)
式中の「コントロールに誘引された」とは、誘引化合物・天敵微生物のいずれも処理していない葉片を、ガラス管の両端に設置した場合の、片側の葉片に誘引されることを示す。
一般に、与えられた1種類の誘引化合物および1種類の天敵微生物を同時処理した際に期待される誘引相乗効果は下記の式2のコルビーの計算式により求められる。
「式2」;E=X+Y-(X*Y)/100
X:誘引化合物Aを処理したときの誘引化合物への誘引率
Y:天敵微生物Bを処理したときの無処理葉片への誘引率
E:誘引化合物Aおよび天敵微生物Bを同時に処理したときに期待される誘引率(誘引率期待値)
Figure JPOXMLDOC01-appb-T000012
Test Example 12 Synergistic effect of attractant compound and natural enemy microorganisms p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-anisidine, m-amino Each single attractant compound selected from methyl benzoate, methyl o-toluate, α-hexylcinnamaldehyde, o-anisaldehyde, β-farnesene, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene A hexane solution containing 1 μg weight was prepared. After impregnating the solution with filter paper, it was placed at one end in a glass tube, and in addition, a kidney leaf piece cut into a certain area (1 cm 2 ) was placed at the inner end of the tube on the same side as bait for thrips.
On the other hand, after dipping green beans in a solution of each single natural enemy microorganism diluted to each practical concentration, selected from the natural enemy microorganisms currently used in general, cut into the same area as the above kidney leaf pieces, The attracting compound was placed at the inner end of the other glass tube without treatment. At this time, the end where the attracting compound was installed was defined as a treatment zone. Thereafter, 20 adult females of Thrips thrips per tube were inserted from one side of the glass tube to the vicinity of the center of the tube, and sponges were plugged at both ends of the tube. The case where thrips were localized in the treatment area 30 minutes after the start of the test was defined as attracted. A similar test was performed a plurality of times, and the attraction rate to the attracting compound was calculated using Equation 1 based on the number of individuals attracted by the attracting compound. The results are shown in Table 12.
“Formula 1”: Attracting ratio to attracting compound = (number of individuals attracted to treatment area−number of individuals attracted to control) * 100 / (number of tests)
“Attracted by control” in the formula indicates that a leaf piece not treated with any attracting compound or natural enemy microorganism is attracted to a leaf piece on one side when both ends of the glass tube are installed.
In general, the attractive synergistic effect expected when a given kind of attracting compound and one kind of natural enemy microorganism are treated at the same time can be obtained by the Colby calculation formula (2) below.
“Formula 2”; E = X + Y− (X * Y) / 100
X: Attraction rate to the attracting compound when the attracting compound A is treated Y: Attraction rate to the untreated leaf piece when the natural enemy B is treated E: Expected when the attracting compound A and the natural enemy B are treated simultaneously Attraction rate (attraction rate expected value)
Figure JPOXMLDOC01-appb-T000012
 本発明によれば有害節足動物誘引化合物および有害節足動物防除用天敵を用いて、有害節足動物に対して効果的に防除しうる方法を提供することができる。施設栽培ハウス内および露地での害虫防除技術において、安全・良質な農産物を安定生産させるために極めて有用である害虫防除体系の提案方法等を汎用技術化することができる。本発明により、化学的に合成された殺虫活性化合物の使用を必要最小限に抑えることが可能となる。 According to the present invention, it is possible to provide a method capable of effectively controlling harmful arthropods using the harmful arthropod-attracting compound and the harmful arthropod-controlling natural enemy. In the pest control technology in the facility cultivation house and in the open field, a proposal method of a pest control system that is extremely useful for stably producing safe and high-quality agricultural products can be made into a general-purpose technology. The invention makes it possible to minimize the use of chemically synthesized insecticidal active compounds.

Claims (14)

  1.  有害節足動物に対して誘引性を有する化合物よりなる群から選ばれる少なくとも1種の化合物と、該有害節足動物に対する天敵よりなる群から選ばれる少なくとも1種の天敵を含んでなる、有害節足動物の行動を制御するための組み合わせであって、前記化合物と天敵が、処置を必要とする場所で前記有害節足動物を相乗的に誘引する態様で使用される、ことを特徴とする組み合わせ物。 A harmful nodule comprising at least one compound selected from the group consisting of compounds attracting harmful arthropods and at least one natural enemy selected from the group consisting of natural enemies against the harmful arthropods A combination for controlling the behavior of paw animals, wherein the compound and natural enemy are used in a manner that synergistically attracts the harmful arthropods in places where treatment is required. object.
  2.  請求項1に記載の組み合わせ物であって、有害節足動物の行動の制御が有害節足動物を防除するか、または発生を予察することにある、組み合わせ物。 The combination according to claim 1, wherein the control of behavior of harmful arthropods is to control harmful arthropods or to predict their occurrence.
  3.  請求項1または2に記載の組み合わせ物であって、前記化合物が動物由来のカイロモン、植物由来のカイロモンおよびそれらの類縁体からなる群より選ばれる、組み合わせ物。 The combination according to claim 1 or 2, wherein the compound is selected from the group consisting of animal-derived kairomones, plant-derived kairomones, and analogs thereof.
  4.  請求項1または2に記載の組み合わせ物であって、前記化合物が植物由来のカイロモンおよびその類縁体からなる群より選ばれる、組み合わせ物。 The combination according to claim 1 or 2, wherein the compound is selected from the group consisting of plant-derived kairomones and analogs thereof.
  5.  請求項1または2に記載の組み合わせ物であって、前記化合物がp-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、ゲラニオール、リナロール、ネロール、シトロネロール、o-アニスアルデヒド、β-ファルネセン、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、オイゲノール、3-フェニルプロピルアルデヒド、シンナムアルデヒド、ユーカリプトール、スクアレン、α-ヘキシルシンナムアルデヒドからなる群より選ばれる、組み合わせ物。 3. The combination according to claim 1, wherein the compound is p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, o-anisaldehyde, β-farnesene, methyl anthranilate, benzoic acid. Group consisting of methyl acid, o-aminoacetophenone, o-anisidine, methyl m-aminobenzoate, methyl o-toluate, eugenol, 3-phenylpropylaldehyde, cinnamaldehyde, eucalyptol, squalene, α-hexylcinnamaldehyde A combination that is chosen more.
  6.  請求項1または2に記載の組み合わせ物であって、前記化合物がp-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、ゲラニオール、リナロール、ネロール、シトロネロール、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、α-ヘキシルシンナムアルデヒドからなる群より選ばれる、組み合わせ物。 The combination according to claim 1 or 2, wherein the compound is p-anisaldehyde, benzaldehyde, ethyl nicotinate, geraniol, linalool, nerol, citronellol, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o A combination selected from the group consisting of anisidine, methyl m-aminobenzoate, methyl o-toluate, α-hexylcinnamaldehyde.
  7.  請求項1または2に記載の組み合わせ物であって、前記化合物がp-アニスアルデヒド、ベンズアルデヒド、ニコチン酸エチル、アントラニル酸メチル、安息香酸メチル、o-アミノアセトフェノン、o-アニシジン、m-アミノ安息香酸メチル、o-トルイル酸メチル、α-ヘキシルシンナムアルデヒドからなる群より選ばれる、組み合わせ物。 The combination according to claim 1 or 2, wherein the compound is p-anisaldehyde, benzaldehyde, ethyl nicotinate, methyl anthranilate, methyl benzoate, o-aminoacetophenone, o-anisidine, m-aminobenzoic acid. A combination selected from the group consisting of methyl, methyl o-toluate, and α-hexylcinnamaldehyde.
  8.  請求項1から7のいずれかに記載の組み合わせ物であって、前記天敵が捕食性天敵、寄生性天敵、天敵微生物からなる群より選ばれる、組み合わせ物。 The combination according to any one of claims 1 to 7, wherein the natural enemy is selected from the group consisting of predatory natural enemies, parasitic natural enemies, and natural enemy microorganisms.
  9.  請求項1から7のいずれかに記載の組み合わせ物であって、前記天敵が捕食性天敵であるスワルスキーカブリダニ、チリカブリダニ、ミヤコカブリダニ、キイカブリダニ、ヘヤカブリダニ、リモニカスカブリダニ、ククメリスカブリダニ、ニセラーゴカブリダニ、コウヅケカブリダニ、デジェネランスカブリダニ、ショクガタマバエ、タイリクヒメハナカメムシ、ナミテントウ、アリガタシマアザミウマ、アカメガシワクダアザミウマ、ヒメカメノコテントウ、タバコカスミカメ、クロヒョウタンカスミカメ、ヤマトクサカゲロウ、オオメカメムシからなる群、および寄生性天敵であるオンシツツヤコバチ、イサエアヒメコバチ、ハモグリコマユバチ、コレマンアブラバチ、サバクツヤコバチ、ハモグリミドリヒメコバチ、チャバラアブラコバチ、コナガサムライコマユバチからなる群、および天敵微生物であるパスツーリアペネトランス、チャハマキ顆粒病ウイルス、リンゴコカクモンハマキ顆粒病ウイルス、スタイナーネマカーポカプサエ、スタイナーネマグラセライ、モナクロスポリウムフィマトパガム、ボーベリアブロンニアティ、ペキロマイセスフモソロセウス、ボベリアバシアーナ、バーティーシリウムレカニ、ペキロマイセステヌイペス、ハスモンヨトウ核多角体病ウイルス、アグロバクテリウムラジオバクター、非病原性エルビニアカロトボーラ、バチルスズブチリス、タラロマイセスフラバス、バチルスシンプレクス、トリコデルマアトロビリデ、シュードモナスフルオレッセンス、コニオチリウムミニタンス、ズッキーニ黄斑モザイクウイルス、バチルスチューリンゲンシスからなる群より選ばれる、組み合わせ物。 The combination according to any one of claims 1 to 7, wherein the natural enemies are predatory natural enemies, such as Swarski spider mites, dust mites, Miyako spider mites, spider mites, spider mites, rimonica spider mites, fowl mites, fake Lago burdock mites, Koji burdock mites, degeneracy burdock mites, shrimp flies, terrestrial spruce winged beetle, nami tento, arigata thrips, red whale thrips, hime winged tents, cigarette mosquitoes, black flies And the parasitic natural enemies, Onsutachi wasp, Isaea himebee, Hamokogurimabachi, Koreman abachi, Sabakakusa bee, Hagomi-midori bee, Chabara abura The group consisting of the bees, the longhorn beetle, and the natural enemies, Pasteuria penetrans, chahamaki granule disease virus, apple coconut spider granule disease virus, steiner nematode carpocapsae, steiner magra serai, monacrosporium fimatopagam, Boberi Ablonniati, Pekir Myces fumosoroseus, Boberia Bassiana, Verticillium regani, Peky Mycestenuipes, Lotus stalk nuclear polyhedrosis virus, Agrobacterium radiobacter, Nonpathogenic Erbiniaca Rotobola, Bacillus subtilis, Talaromyces flavus, Bacillus simplex, Trichoderma atrovilide, Pseudomonas fluorescens, Coniochilium minitance, Zucchini macular mosaic virus, Bacillus Selected from the group consisting of Ringenshisu, combinations thereof.
  10.  請求項1から7のいずれかに記載の組み合わせ物であって、前記天敵が捕食性天敵であるスワルスキーカブリダニ、チリカブリダニ、ミヤコカブリダニ、キイカブリダニ、ヘヤカブリダニ、リモニカスカブリダニ、ククメリスカブリダニ、ニセラーゴカブリダニ、コウヅケカブリダニ、デジェネランスカブリダニ、ショクガタマバエ、タイリクヒメハナカメムシ、ナミテントウ、アリガタシマアザミウマ、アカメガシワクダアザミウマ、ヒメカメノコテントウ、タバコカスミカメ、クロヒョウタンカスミカメ、ヤマトクサカゲロウ、オオメカメムシからなる群、および寄生性天敵であるオンシツツヤコバチ、イサエアヒメコバチ、ハモグリコマユバチ、コレマンアブラバチ、サバクツヤコバチ、ハモグリミドリヒメコバチ、チャバラアブラコバチ、コナガサムライコマユバチからなる群より選ばれる、組み合わせ物。 The combination according to any one of claims 1 to 7, wherein the natural enemies are predatory natural enemies, such as Swarski spider mites, dust mites, Miyako spider mites, spider mites, spider mites, rimonica spider mites, fowl mites, fake Lago burdock mites, Koji burdock mites, degeneracy burdock mites, shrimp flies, terrestrial spruce winged beetle, nami tento, arigata thrips, red whale thrips, hime winged tents, cigarette mosquitoes, black flies And the parasitic natural enemies, Onsutachi wasp, Isaea himebee, Hamokogurimabachi, Koreman abachi, Sabakakusa bee, Hagomi-midori bee, Chabara abura Drumstick, selected from the group consisting of Plutella xylostella Samurai Braconidae, combinations thereof.
  11.  請求項1から7のいずれかに記載の組み合わせ物であって、前記天敵が捕食性天敵であるスワルスキーカブリダニ、チリカブリダニ、ミヤコカブリダニ、キイカブリダニ、ヘヤカブリダニ、リモニカスカブリダニ、ククメリスカブリダニ、ニセラーゴカブリダニ、コウヅケカブリダニ、デジェネランスカブリダニ、ショクガタマバエ、タイリクヒメハナカメムシ、ナミテントウ、アリガタシマアザミウマ、アカメガシワクダアザミウマ、ヒメカメノコテントウ、タバコカスミカメ、クロヒョウタンカスミカメ、ヤマトクサカゲロウ、オオメカメムシからなる群より選ばれる、組み合わせ物。 The combination according to any one of claims 1 to 7, wherein the natural enemies are predatory natural enemies, such as Swarski spider mites, dust mites, Miyako spider mites, spider mites, spider mites, rimonica spider mites, fowl mites, fake From Largo burdock mites, Koji burdock mites, Degenerence bursid mites, Drosophila wings, Timber winged beetles, Nami Tento, Arigata thrips, Akamegasiwada thrips, Hime damselfia tents, Tobacco mosquitoes, Black wings Selected combination.
  12.  請求項1から11のいずれかに記載の組み合わせ物であって、選ばれる誘引性を有する化合物の処理量が、1種の化合物あたり0.0003~12kg/haである、組み合わせ物。 The combination according to any one of claims 1 to 11, wherein the selected amount of the compound having an attractive property is 0.0003 to 12 kg / ha per one compound.
  13.  請求項1から12のいずれかに記載の組み合わせ物であって、選ばれる有害節足動物に対する天敵の放飼量が、捕食性天敵あるいは寄生性天敵の場合は、0.01~100頭/m2、天敵微生物の場合は1~5kg/haである、組み合わせ物。 The combination according to any one of claims 1 to 12, wherein the amount of natural enemies released to the selected arthropod is 0.01 to 100 heads / m in the case of predatory natural enemies or parasitic natural enemies. 2. A combination of 1 to 5 kg / ha for natural enemy microorganisms.
  14.  請求項1から13いずれかに記載の組み合わせ物を用いた有害節足動物防除法または有害節足動物予察法。 A harmful arthropod control method or a harmful arthropod prediction method using the combination according to any one of claims 1 to 13.
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