WO2014145768A3 - Use of non-fungal 5' utrs in filamentous fungi - Google Patents

Use of non-fungal 5' utrs in filamentous fungi Download PDF

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Publication number
WO2014145768A3
WO2014145768A3 PCT/US2014/030589 US2014030589W WO2014145768A3 WO 2014145768 A3 WO2014145768 A3 WO 2014145768A3 US 2014030589 W US2014030589 W US 2014030589W WO 2014145768 A3 WO2014145768 A3 WO 2014145768A3
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WO
WIPO (PCT)
Prior art keywords
expression
utrs
present disclosure
fungal
recombinant
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PCT/US2014/030589
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French (fr)
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WO2014145768A2 (en
Inventor
Nicholas J. RYDING
Danielle I. BINGER
Thomas D. GOLDMAN
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Bp Corporation North America Inc.
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Application filed by Bp Corporation North America Inc. filed Critical Bp Corporation North America Inc.
Publication of WO2014145768A2 publication Critical patent/WO2014145768A2/en
Publication of WO2014145768A3 publication Critical patent/WO2014145768A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present disclosure is directed to the use of non- fungal 5' UTRS to enhance recombinant expression in filamentous fungal cells. In certain aspects, the present disclosure provides an expression cassette useful for the expression of polypeptide in filamentous fungal cells. Also provided herein, are vectors and recombinant filamentous fungal cells comprising the expression cassettes of the present disclosure, and methods of making and using the same for recombinant polypeptide expression.
PCT/US2014/030589 2013-03-15 2014-03-17 Use of non-fungal 5' utrs in filamentous fungi WO2014145768A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361799962P 2013-03-15 2013-03-15
US61/799,962 2013-03-15

Publications (2)

Publication Number Publication Date
WO2014145768A2 WO2014145768A2 (en) 2014-09-18
WO2014145768A3 true WO2014145768A3 (en) 2014-12-04

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Family Applications (1)

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PCT/US2014/030589 WO2014145768A2 (en) 2013-03-15 2014-03-17 Use of non-fungal 5' utrs in filamentous fungi

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US9404101B2 (en) * 2011-08-24 2016-08-02 Novozymes, Inc. Methods for obtaining positive transformants of a filamentous fungal host cell
BR112014004190A2 (en) 2011-08-24 2017-03-01 Novozymes Inc method for constructing a filamentous fungal strain, filamentous fungal strain, method for producing multiple recombinant polypeptides, and tandem construct
CN105695439B (en) * 2014-11-27 2023-08-01 丰益(上海)生物技术研发中心有限公司 Recombinant expression method of beta-glucosidase gene
CN107723380A (en) * 2017-11-30 2018-02-23 福建省农业科学院植物保护研究所 A kind of stem rot of sweet potato bacterium LAMP detection primer and its application
CN108504781A (en) * 2018-06-01 2018-09-07 西南科技大学 A kind of rhizome of Chinese monkshood mosaic disease pathogen method for identifying molecules and control and application
CN112779166B (en) * 2019-11-08 2022-08-30 青岛蔚蓝生物集团有限公司 Trichoderma reesei strain with high lipase yield and application thereof

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