WO2013058600A2

WO2013058600A2 - Platelet extraction kit

Info

Publication number: WO2013058600A2
Application number: PCT/KR2012/008601
Authority: WO
Inventors: 양경식
Original assignee: 최득수
Priority date: 2011-10-19
Filing date: 2012-10-19
Publication date: 2013-04-25
Also published as: WO2013058600A3

Abstract

The present invention relates to a platelet extraction kit which can separate serum and platelets again at the state where blood is separated by centrifugation, so as to extract and inject only platelets in an easy manner. The kit comprises: a tubular rod arranged to penetrate in a vertical direction through the center of a cap that closes an upper portion of a container; and a piston coupled to the rod and embedded within the container. The rod has an upper portion closed by a seal cap so that the inside of the container is sealed. The coupled portion between the rod and the piston has a flow channel penetrating in a vertical direction so that the serum and platelets in the container are separated in a vertical direction by the upward and downward movement of the piston, thus easily extracting only non-necrotic pure platelets.

Description

Platelet eviction kit.

The present invention relates to a platelet eviction kit for discharging only platelets enriched in blood, wherein the platelet eviction kit allows separation of serum and platelets in a state in which blood is separated by centrifugation to facilitate the eviction of only platelets. It is about.

In general, blood transmits oxygen taken from the lungs and nutrients absorbed from the digestive tract to the whole body and carries carbon dioxide or waste products made from cells to excrete out of the body through the lungs, kidneys, and skin. Likewise, it plays a role in living organisms that transfer heat from a place of heat production to another part to equalize the distribution of body heat, and such blood is composed of red blood cells and platelet serum, among which platelets Silver is rich in growth factors and is used for therapeutic and cosmetic purposes.

In other words, when the platelets are administered to the body, they stimulate the stem cells to help the cells to be generated, thereby regenerating the skin as well as maintaining the elasticity of the skin, which is used for cosmetic purposes as well as for cosmetic purposes.

However, these platelets contain only a very small amount in the collected blood and have high viscosity, making it difficult to remove them because they adhere to erythrocytes. When platelets are administered to the human body when platelets are not properly separated, erythrocytes enter the human body Inflammation or pain, or if the serum enters the human body will cause side effects such as skin necrosis is required to remove only pure platelets.

Therefore, in recent years, the blood is separated by centrifugation so that the red blood cells with the highest specific gravity are located in the lower layer, and platelets are separated on the upper side, and serum is located on the upper side of the platelet so that only the platelets located in the center can be removed. Although platelet eviction kits can be manufactured and used as shown in FIGS. 15 and 16, this is difficult to remove only pure platelets in a container, especially when other substances are included together during the eviction of platelets. It can cause side effects.

In addition, such platelet eviction kits are oxidized when combined with oxygen in a centrifuged state, and thus platelets are necrotic, so only live platelets cannot be sufficiently secured, and therefore, the reaction sensitive skin cannot be used for fear of side effects and platelet necrosis. It can only be used if it can be used even in a limited state, so the scope of use is very limited.

In addition, when the platelet is directly sucked from the container in which the blood is separated, the platelet is necrosed due to the instantaneous pressure of the needle, so the use of the platelet is inevitably limited.

In addition, even if platelets are alive in the blood reservoir by solving these problems, as platelets are alive, the platelets and serum are easily mixed again even when separated from serum by centrifugation, so only high purity platelets are evicted. There is a difficulty in doing so, and thus, the field of use of platelets is limited, and there is still a need for a technique for excluding pure platelets.

Therefore, the present invention allows the storage and centrifugation of the blood in a sealed state, and also through the structural improvement of the platelet extraction kit to easily separate the platelets and serum in the centrifuged blood to extract only the concentrated platelets, such The platelet can be administered to the patient without necrosis in the process of eviction of platelets, and reused.

To this end, the present invention allows the piston to be introduced into the interior of the container body, and the rod made of the tubular body is formed so as to penetrate the upper part of the container body so as to penetrate the center of the cap so that the piston and the flow path embedded in the container body are formed. In this state, the upper portion of the rod made of the tubular body is closed by a cap to allow the inside of the container to be in a sealed state, and when the rod and the piston are first coupled, the centrifugal flow is formed through the upper and lower portions of the piston. By separating the blood first by separation, the serum and platelets are separated by the lowering of the piston and the blockage of the flow path by the manipulation of the rod so that only pure platelets that are not necrotic can be easily removed.

In addition, by changing the coupling structure of the piston and the rod to the screw tightening structure, it is possible to use repeatedly, as well as to be able to extract precisely plated platelets and precise operation even in repeated use.

Therefore, the present invention is stored in a closed state until administered to the human body to prevent the necrosis of platelets due to oxidation, so that only live pure platelets can be used for various purposes, depending on the purpose of use, and also live platelets and serum during such eviction process It is easy to evoke only platelets while easily separating the ease of use, as well as to enhance the therapeutic and cosmetic effects, it can be easily performed without various side effects.

1 is a perspective view showing a state before use of the platelet extraction kit of the present invention.

Figure 2 is an exploded perspective view of Figure 1;

3 is a longitudinal cross-sectional view of FIG.

4a to 4g are cross-sectional views showing the use state of the present invention.

Figure 5 is a perspective view of a state in which the anti-press device is removed from the platelet extraction kit of the present invention.

Figure 6 is a perspective view showing the opening and closing state of the flow path of the rod and the piston of the present invention.

7 and 8 are a perspective view of another coupled state of the piston and the rod of the present invention.

Figure 9 is an assembled perspective view showing another coupling structure of the piston and the rod of the present invention.

Fig. 10 is a sectional view of the assembled state of Fig. 9;

Fig. 11 is a sectional view of the separated state of Fig. 9;

12 is an open / closed state diagram of still another flow path of the piston and the rod of the present invention.

13A to 13F are cross-sectional views showing the use states of FIG. 9 separately.

Fig. 14 is a sectional view of the assembled state of Fig. 9;

Figure 15 is a perspective view of a conventional platelet extraction kit

Figure 16 is a schematic view of the use state of the conventional platelet extraction kit

The present invention is formed of a container body 10 containing the collected blood, a cap 20 for sealing the upper portion of the container body 10 to be sealed, and a tubular body which is coupled to penetrate the cap 20 up and down. The rod 30 is connected to the rod 30 while being detached from the rod 30, and a piston 40 inserted into the container body 10 coupled to maintain airtightness, and an upper portion of the rod 30 made of the tubular body. It consists of a closed cap 50 to seal the interior of the container body 10 to seal the piston 40 by the operation of the rod while the platelet eviction kit is separated from the platelets and serum through the flow path is not mixed.

Hereinafter, with reference to the accompanying drawings, preferred embodiments of the present invention will be described.

As shown in Figs. 1 to 3, the present invention includes a container body 10 containing blood collected therein, a cap 20 which closes the upper part of the container body 10, and a center of the cap 20. Of the rod 30 formed of a tubular body so as to penetrate up and down, the piston 40 inserted into the container body 10 detachably coupled to the rod 30, and the rod 30 of the tubular body. Closing the upper portion is a configuration consisting of a closed cap 50 to seal the inside of the container body 10.

At this time, the inside of the container body 10 is sealed to block the outside air in the process of storing or centrifuging the blood or discharging platelets to prevent platelet necrosis. By lowering the piston 40 by separating the serum and platelets inside the container body 10 separated by centrifugation by separating the upper and lower, it is to be able to easily extract only the pure platelets not necrotic.

In this case, in order to separate and prevent platelets from being mixed, the rod 30 forms a cutout 31 at a lower end thereof and connects the through hole 41 formed at the center of the piston 40 to the rod 30 and the piston ( The flow path is formed so as to penetrate the upper and lower portions of the piston 40 in the state in which the coupling 40 is connected, wherein the piston 40 is formed of a rigid material in order to prevent arbitrary separation when the rod is coupled, The packing 45 having excellent plate force is coupled to the groove frame formed on the outer circumferential surface thereof so that the packing 45 formed on the outer circumferential surface of the piston 40 closely adheres to the inner wall of the container body 10 when the piston 40 moves up and down. It is to be able to be lifted while maintaining the state.

In addition, the connection coupling of the rod 30 and the piston 40 forms a locking jaw (32, 42) to be engaged to correspond to each other by the pressure of the rod 30 to assemble the two by force fitting each other, As shown in FIGS. 6 and 7 by forming a protruding wing 33 to the upper portion of the engaging jaw 32 of the rod 30 to engage with the wing inner portion 43 formed on the upper surface of the piston 40. As described above, opening and closing of the flow path is prevented by preventing the incision 31 from being mixed with the first separated serum and platelets in a state where the blood by centrifugation is separated by the opening of the flow path by the incision 31. It is possible.

At this time, the piston 40 is formed as the stopper 44 to protrude on the upper surface of the blade inner blade 43 as shown in Figure 8 engaging the engaging blade 33 of the rod 30 is engaged with the blade inner blade 43. Even if it is rotated in order to be locked to the stopper 44 is to be controlled to be accurately positioned in the open or closed position of the flow path.

In this state, the protruding wing 33 and the wing inner part 43 are easily fitted into position by pressing the protruding wing 33 onto the wing inner part 43.

9 to 11 illustrate a connection relationship between the piston and the rod according to another configuration, and the opening hole 31a penetrates in a lateral direction at a front end thereof engaged with the piston 40a of the rod 30a made of a tubular body. This is formed to have a structure in which the flow path is opened or blocked according to the tightening state by screwing the rod (30a) and the piston (40a), the platelet is the platelet in the state where the blood is centrifuged and separated into platelets and serum In order to lower to the position, the opening hole 31a is opened to lower the piston 40a. In this state, the platelet is further tightened in order to withdraw the platelets from mixing with the serum. ) To block serum from seeping out.

For the tightening operation, the rod 30a and the piston 40a form

spiral portions

32a and 42a to correspond to each other to tighten or loosen the screw holes in order to block or open the opening hole 31a. By rotating 30a, the opening hole 31a can be simply blocked or opened.

In addition, due to the characteristics of the screw tightening method made of a multi-layer as described above, when the platelets are ejected together in the tightening method than the state in which the rod 30 and the piston 40 are fitted as described above precisely that the serum is introduced together There is an effect that can be blocked.

In addition, when the flange 33a for blocking the upper surface of the piston 40a is formed at the upper end of the spiral portion 32a of the rod 30a, the rod 30a and the piston 40a are tightly sealed. Of course, the tightening effect is to be interrupted.

And when the packing (34a) is mounted on the lower end of the flange (33a) inserted and assembled in this way, when the rod (30a) is locked by locking the flange (33a) by pressing the packing (34a) while the rod (30a) and the piston ( 40a) is more closely contacted, and when the platelets are extracted from the platelets and serum by the piston 40a, serum is infiltrated at the source and only pure platelets can be removed.

Referring to the drawing process of such platelets with reference to the drawings shown in Figure 13a to Figure 13f as follows.

When the blood is injected into the container body 10 through the sealing cap 50 fitted to the upper portion of the rod 30a as shown in FIG. 13a, and centrifuged while the blood is filled as shown in FIG. 13b, at the bottom as shown in FIG. Erythrocytes are separated in a layered manner so that platelets are placed on the upper layer and serum is placed on the platelets.

Subsequently, when the piston 40a is pushed down by pushing the rod 30a, the serum rises to the upper portion of the piston 40a through the opening hole 31a of the rod 30a. The piston 40a will be pushed to the position where it is located.

And when the serum is raised to the upper portion of the piston (40a) by rotating the rod (30a) as shown in Figure 13e to lock the opening hole (31a) is to isolate the serum and platelets, in that state rod (30a) The flange (33a) and the packing (34a) formed in the) is to be in close contact with the upper portion of the piston 40a to maintain the isolated state more closely.

Then, as shown in Figure 13f to remove the sealing cap 50 is inserted into the upper portion of the rod (30a) by inserting the syringe will be able to suck only platelets with the syringe through the rod (30a) made of tubular body.

Therefore, the blood is stored in a sealed state from the moment of injecting blood to the platelet eviction kit until administered to the human body to prevent the necrosis of platelets due to oxidation, and also to easily separate the live platelets and serum during the eviction process. It is convenient to use, and can be used in various ways for treatment or cosmetic purposes without side effects.

At this time, the structure of the piston (40a) can achieve the same purpose even when integrally molded into a single body, but more preferably more flexible and elastic force than the insert member to the outside of the insert member 43a having a certain hardness It is preferable that the piston body (44a) made of an elastic material having a wrap is made, and in this case the structure is made of a double structure of the insert member (43a) and the piston body (44a) is the insert member (43a) ) And the piston body 44a correspond to each other to form a fitting portion 45a and assemble by force fitting, or insert molding the piston body 44a with the insert member 43a inserted into the molding frame, It is not to be separated.

The outer peripheral surface of the piston body 44a which is in close contact with the inner wall 11 of the vessel body 10, that is, the piston body 44a is formed with an inner portion 44aa and the upper and lower portions of the inner portion 44aa. By forming the protruding frame 44bb to protrude so that the protruding frame 44bb is in close contact with the inner wall 11 of the container body 10, the piston 40 is double-sealed to the inner wall of the container body 10 to maintain a closed state. Keeping is desirable.

That is, the protrusions 44bb respectively formed on the upper and lower ends of the piston 40a maintain the state of being in close contact with the inner wall 11 of the container body 10 so that the piston 40a can be raised and lowered. As well as sealing, the piston 40a is raised and lowered while maintaining the horizontal state at a uniform pressure without causing an eccentricity to either side, so that the platelets can be more stably collected and expelled.

In addition, the rods (30, 30a) is preferably formed by the pressing portion 35 on the upper end to facilitate the pressing operation and also to provide easy molding by separating the rods (30, 30a) formed by the tubular body The

rods

30 and 30a penetrate up and down through the through-holes 21 formed in the center of the cap 20 to facilitate assembly.

In addition, the platelet eviction kit is circulated by inserting the pressing preventing device 60 having the fitting groove 61 formed between the lower surface of the pressing portion 35 and the upper surface of the cap 20 of the

rods

30 and 30a from the side. In addition, in order to increase the efficiency of platelet extraction in the process of management to prevent the rod (30, 30a) that is set at a certain position to be pressed arbitrarily.

At this time, it is preferable to form the protruding portion 62 to prevent the pressing portion 35 of the

rods

30 and 30a from being arbitrarily rotated to one side of the pressing preventing tool 60.

4A to 4G of the present invention illustrate the process of depleting platelets in the coupling structure of the rod 30. Referring to the drawings, the process of depleting platelets is as follows.

Injecting blood into the platelet eviction kit through the sealing cap 50 fitted to the upper portion of the rod 30 as shown in Figure 4a, and centrifugation in the blood filled state as shown in Figure 4b and red blood cells and platelets as shown in Figure 4c Serum is separated in layers up and down.

Thereafter, as shown in FIG. 4D, the anti-pressing tool 60 is removed, and when the pressing part 35 of the rod 30 is pressed, the piston 40 descends while serum is formed between the rod 30 and the piston 40. It is raised to the upper portion of the piston 40 through the flow path, in this case, the rod 30 and the piston 40 is to maintain the open state.

In addition, as shown in FIG. 4E, when the serum is raised to the upper portion of the piston 40, when the rod 30 is rotated and pressed, the protruding blade 33 of the rod 30 is inserted into the blade inner portion 43 of the piston 40. As the flow path is blocked, serum and platelets are completely separated as shown in FIG. 4F.

Thereafter, as shown in FIG. 4G, after removing the sealing cap 50 fitted to the rod 30 and inserting the syringe, platelets are lifted to the upper portion of the syringe through the tubular body of the rod 30.

At this time, since the inside of the container body 10 maintains a closed state, when the syringe is plugged in, only platelets are sucked by the sealing pressure, and thus only pure platelets can be removed.

On the other hand, the piston (40, 40a) is formed when the inclined surface (41a) so as to gradually narrow the shape of the insertion hole 41 formed in the center in order to have the coupling and the flow path of the rod (30, 30a) from the lower side piston When the drop of (40, 40a), the platelets are concentrated in the center to facilitate the eviction of platelets.

Therefore, it is stored in a sealed state until it is administered to the human body to prevent the necrosis of platelets due to oxidation, so that only live pure platelets can be used for various purposes depending on the purpose of use, and also the living platelets and serum are easily separated during such extraction. However, only platelets can be easily displaced, so that the convenience of use as well as the treatment effect and the beauty effect can be enhanced, and can be easily performed without various side effects.

On the other hand, if the cap 20 is formed in the inner groove 22 to the outside through-hole 21 is formed in the center can be used to extract only the serum alone if it is necessary to use the serum in the blood centrifuged state At this time, the shape of the indentation groove 22 may be formed in the shape of a circular frame, but more preferably it is preferable to form a groove made alone for easy molding.

In this case, since the inside of the container body 10 is kept in a closed state, two grooves are formed on both sides, so that one side of the container is plugged into the syringe and the serum is extracted, and the other side is inserted into the air suction hole so that the air can be introduced. This is desirable.

Claims

A container body 10 containing the collected blood,

A cap 20 for sealing the upper portion of the container body 10 to be sealed,

A rod 30 formed of a tubular body coupled to penetrate the cap 20 upward and downward,

A piston (40) inserted into and detached from the rod (30) and inserted into the container body (10) which is coupled to maintain airtightness;

Separation of platelets and serum through the flow path while the piston 40 is lowered by manipulating the rod made of a closing cap 50 to close the upper portion of the rod 30 made of tubular body to seal the inside of the container body 10. A platelet eviction kit that is made from mixing.
According to claim 1, In order to separate and prevent the platelets from being mixed, the rod 30 is formed by the incision (31) at the lower end by connecting the through hole 41 formed in the center of the piston (40) 30) and platelet eviction kit for forming a flow path to penetrate the upper and lower portions of the piston 40 in a state in which the piston 40 is coupled.
The piston (40) according to claim 2, wherein the piston (40) is formed of a hard material to prevent random separation when the rod (40) is coupled to the rod, and the packing (45) having excellent plate force is coupled to the groove frame formed on the outer circumferential surface thereof. A platelet eviction kit, wherein the packing 45 formed on the outer circumferential surface of the piston 40 is lifted while maintaining the state in which the packing 45 is in close contact with the inner wall of the container body 10.
The method of claim 2, wherein the connection of the rod 30 and the piston 40 is formed by engaging the jaw (32, 42) to be engaged with each other by the pressure of the rod 30 to force the two together The upper part of the engaging jaw 32 of the rod 30 to form a protruding wing 33 to engage with the wing inner portion 43 formed on the upper surface of the piston 40 to be combined by blood by centrifugation The platelet eviction kit which enables the opening and closing of the flow path by preventing the incision 31 so as not to mix the first separated serum and platelets in the state of being separated by the opening of the flow path by the incision 31. .
According to claim 4, The piston 40 is formed in the stopper 44 to protrude on the upper surface of the blade inner portion 43 so that the protruding blade 33 of the rod 30 is engaged with the blade inner portion 43 Platelet eviction kit that can be accurately positioned in the open or closed position of the flow path while being caught to the stopper (44) when it is rotated.
According to claim 1, In order to separate and prevent the platelets from being mixed, the opening end 31a is formed to penetrate in the lateral direction at the front end coupled with the piston 40a of the rod 30a made of tubular body 30a ) And a platelet eviction kit having a structure in which the flow path is opened or blocked according to the tightening state by screwing the piston and the piston (40a).
The method of claim 6, wherein the rod (30a) and the piston (40a) to form a spiral portion (32a, 42a) to correspond to each other to tighten or loosen by a screw tightening method to block or open the opening hole (31a) Platelet eviction kit.
8. A flange (33a) for blocking the upper surface of the piston (40a) is formed at the upper end of the spiral portion (32a) of the rod (30a) to further seal the rod (30a) and the piston (40a). Tightly platelet eviction kit.
According to claim 8, when the packing (34a) is mounted on the lower end of the flange (33a) to rotate the rod (30a) to lock the flange (33a) by pressing the packing (34a) while pressing the rod (30a) A platelet eviction kit in which the piston (40a) is in close contact.
The piston body (44a) according to any one of claims 6 to 9, wherein the piston (40a) is made of an elastic material having flexibility and elasticity than that of the insert member to the outside of the insert member (43a) having a constant hardness. Wrapped around
The method of claim 10, wherein the insert member 43a and the piston body 44a to form a fitting portion 45a to correspond to each other in order to have a double structure of the insert member (43a) and the piston body (44a) A platelet eviction kit for assembling by force fitting or insert molding the piston body (44a) in a state where the insert member (43a) is inserted into the molding die so as not to be separated.
The method of claim 11, wherein the outer periphery of the piston body (44a) while forming the inner portion (44aa) protruding frame (44bb) to protrude up and down of the inner portion (44aa) protruding frame (44bb) The platelet eviction kit, which is in close contact with the inner wall (11) of the container body (10) to maintain a sealed state by double sealing the piston (40) to the inner wall of the container body (10).
10. The platelet extraction kit according to any one of claims 1 to 9, wherein the rods (30, 30a) are formed at the upper end with a pressing portion (35) to facilitate the pressing operation.
15. The rod (30, 30a) formed in the tubular body is formed in an equally separated form to provide ease of molding while the rod (30, 30a) is formed in the through hole (21) formed in the center of the cap (20). Platelet eviction kit, easy to penetrate down through the assembly.
The platelet according to claim 13, wherein the platelets are formed by inserting the anti-pressing device 60 formed between the lower surface of the pressing part 35 of the rods 30 and 30a and the upper surface of the cap 20 from the side surface thereof. Platelet eviction kit to prevent the rod (30, 30a) that is located at a predetermined position to increase the efficiency of platelet extraction in the process of distributing or managing the eviction kit arbitrarily pressed.
16. The platelet extraction kit according to claim 15, wherein a protrusion (62) is formed on one side of the anti-depressor (60) to prevent the pressing portion (35) of the rod (30, 30a) from being arbitrarily rotated.
10. The piston (40) according to any one of claims 1 to 9, wherein the piston (40, 40a) is formed from the lower side in the form of an insertion hole (41) formed at the center for the engagement of the rods (30, 30a) and the flow path. A platelet eviction kit in which an inclined surface (41a) is formed to be gradually narrowed inclined so that platelets are concentrated in the center when the pistons (40, 40a) are lowered, thereby making platelet extraction easier.
The platelet extraction kit according to claim 1, wherein the cap (20) forms an indentation groove (22) outwardly formed with a through hole (21) in the center thereof so that serum can be used in a state where blood is centrifuged.