WO2013055070A2

WO2013055070A2 - Plasma extraction kit

Info

Publication number: WO2013055070A2
Application number: PCT/KR2012/008148
Authority: WO
Inventors: 류종현
Original assignee: Reu Jong-Hyun
Priority date: 2011-10-10
Filing date: 2012-10-09
Publication date: 2013-04-18
Also published as: WO2013055070A3

Abstract

The present invention relates to a plasma extraction kit comprising: a cylindrical main body portion in which the upper end is opened and the lower end is closed; a funnel portion which is fastened to the inner central portion of the main body portion and is formed into a tapered shape in such a way that the diameter of the central portion is small and the diameters of the both ends gradually decrease as they approach the central portion; a cover portion for closing the upper end of the main body portion; an open/close cap fastened to the cover portion in an openable/closable manner, in which an injection hole, into which a needle of a syringe for injecting the blood extracted from a patient can be inserted, is formed at one lateral side of the open/close cap; and a second centrifuge tube for further extracting plasma from PPP and erythrocyte of the centrifuged blood by second centrifugation. A large amount of erythrocytes among blood components are accommodated in a blood storage portion after centrifugation, and a small amount of PRP can be accommodated in the middle of the funnel portion having a small diameter, thereby facilitating extraction of a small amount of PRP. In addition, even though the amount of erythrocytes depends on an individual, the position of the funnel portion can be controlled by the configuration of a connection wing portion and the funnel portion, and thus it is possible to precisely locate PRP at the funnel portion. Further, a sterilizing gas can be injected into the plasma extraction kit through the injection hole of the open/close cap, thereby facilitating sterilization. Additionally, since opening the open/close cap, injecting blood by inserting the needle of a syringe into the injection hole of the open/close cap, and then pushing the open/close cap so as to seal the same is enabled, contamination is not a large concern when using the same and the invention is convenient to use.

Description

Plasma extraction kit

The present invention relates to a plasma extraction kit, and more particularly, a device used to separate and extract a specific component of blood, to increase the concentration and purity of a specific component to be extracted and to easily extract a specific component from the blood. The present invention relates to a plasma extraction kit that is easy to disinfect and convenient.

In general, blood cells in the blood are mixed with different specific gravity components, when centrifuged to form a layer by each type.

Recently, platelet rich plasma (PRP, Platelet Rich Plasma Platelet Rich Plasma, or 'PRP') has been known to be known for its excellent effect on tissue regeneration. PRP administration is a treatment method that is applied in many fields, such as applied to skin care and skin treatment.

Platelets have a regenerative effect because platelet cells contain abundant growth factors. Injecting high levels of platelets into the treatment area causes platelet growth factors to activate stem cells to regenerate damaged tissue. The higher the concentration of PRP, the greater the therapeutic effect.

The general PRP extraction process first collects blood from the human body and centrifuges it. Several cells are mixed in the blood. When centrifuged, homogeneous cells are collected and layered according to specific gravity. The blood consists of a solution component and a blood cell component (grain cell), which contains red blood cells, white blood cells, platelets, and a very small amount of antibodies, which are mixed and floated in a solution with 0.9% salinity. Red blood cells constitute the majority of the blood cell components.

When the blood is centrifuged, it is separated into three layers. The heaviest red blood cells are collected at the bottom of the centrifuge tube, and platelets and leukocytes are gathered on the upper layer to form a thin layer. Missing solution components are placed. The platelet and leukocytes are concentrated together, and the thin layer is called PRP, and the plasma layer composed only of solution without blood cell granules above is called PPP (Platelet Poor Plasma). .

Several instruments and methods have been proposed for screening double PRP layers.

[Preceding technical literature]

(Patent Document 1) Korean Registered Patent No. 1016166 (Method for separating autologous platelet concentrate)

(Patent Document 2) Korean Registered Patent No. 1043231 (centrifugal separation method and adapter used therein)

(Patent Document 3) Korean Registered Patent No. 1029255 (centrifugal separator)

However, according to the conventional PRP extraction method, there is a problem that high purity PRP extraction is not easy.

That is, there is a problem that red blood cells are included, which should not be included, and the extraction process is very precise, so that the concentration of PRP is not constant according to the extractor, resulting in deviations in the degree of control, and the extraction process requires delicate manipulation. It takes a lot of time, there is a problem that it is difficult to freely control the concentration of the extracted PRP from high concentration to low concentration, there is a problem that the price of the conventional PRP extraction centrifuge tube is very expensive.

When the blood is centrifuged, the PRP appears as a very thin layer, which appears to be thin and widely spread over the entire red blood cell layer directly below, and the thickness of the red blood cell that is collected at the bottom is different depending on the person. Since the ratio of the total volume of the whole blood varies from as little as 25% to as much as 50%, it is not easy to extract high purity PRP.

In order to extract PRP thinly spread on the top of the erythrocyte layer, the needle is inserted into the test tube, and the tip of the needle touches the PRP layer and is extracted by inhaling it. In the process, a significant number of red blood cells are sucked in with the PRP, and these red blood cells are injected into the tissue along with the PRP when the patient is treated.

Erythrocytes sucked into PRP cause two problems. First, the higher the amount of red blood cells, the lower the concentration of PRP, which lowers the therapeutic effect. Second, the patients complain of severe pain after the procedure. Cause. Even with pure PRP, there is some pain after treatment, which lasts as long as 1 day and as long as 1 week. In some cases, narcotic analgesics may be required. When the red blood cells are injected together, the pain becomes more severe. For this reason, several methods are used to prevent the inclusion of red blood cells in PRP extraction. The most commonly used method is to reduce the diameter of the centrifuge tube in the area where the PRP layer is expected to form and to reduce the proportion of PRP. By stacking thicker to reduce the area in contact with the erythrocyte layer is taking a way to extract. When using this method, there is one problem. Even when the same amount of blood is collected from different people and centrifuged into a centrifuge tube, the thickness of the lower red blood cell layer is different due to the different amount of red blood cells as described above. Therefore, the PRP is rarely actually located after centrifugation at the site where the PRP is expected to form.

Therefore, a correction device that adjusts the height of the red blood cell layer in the lower part of the separator to position the PRP in the narrow neck portion should be accompanied. Usually, a screw-shaped stopper in the lower part of the centrifuge tube may be locked or released depending on the situation. Rotating in the direction to control the height of the red blood cell layer. By the way, the centrifuge tube of this type has a great escape pressure of blood in the lower part of the centrifuge tube during the high-speed rotation of 3000-4000RPM, and there is a possibility that blood can flow out through the screw part. For this reason, it is necessary to manufacture additional parts and precise structure to prevent leakage, which leads to an increase in manufacturing cost, which inevitably increases the price of the centrifuge tube, resulting in a higher final treatment amount. And the extraction devices designed like this have a short neck length so that it is very difficult to accurately place the PRP in this part.The result is that the PRP layer is scattered to some extent in the process of moving the layer by rotating the lower screw device. Results in.

In addition, as a conventional method of extracting PRP, a method of using a syringe for serum separation is disclosed, as described in Korean Utility Model Registration No. 20-0273082.

According to the prior art, a piston is inserted into the upper part to be movable, the lower portion is formed with a needle connector is fixed to the needle, the inside is filled with a certain amount of serum separation gel for separating blood and serum therein; A protrusion which protrudes above the needle connector formed in the lower portion of the cylinder and is formed inside the cylinder so that the serum separation gel filled in the syringe is not discharged toward the needle connector where the needle is fixed; A lower stopper which is fixed to the lower part of the needle connector when the needle is removed from the needle connector of the cylinder to which the needle is fixed, and a seal which seals the needle connector protrudes upward; When the piston movably fixed to the cylinder is removed is fixed to the open top of the cylinder, a serum separation syringe comprising an upper stopper is formed on the surface in close contact with the cylinder is formed.

According to the prior art, after a certain amount of blood is injected into the cylinder, the cylinder is sealed by an upper cap.

On the other hand, medical devices such as blood separation syringes, plasma extraction kits and syringes used for blood extraction are very important factors as well as sterilization and sterilization so that bacteria do not penetrate the extracted blood.

As a disinfection method of such a medical device, a method of sterilization by high temperature steam, a method of sterilizing by adding EO gas, and a method of sterilizing by irradiating gamma rays are mainly used. In the case of sterilization by high temperature steam, not only is it difficult to apply to plastic products, but also has a problem of being difficult to sterilize in a sealed state. It is difficult to disinfect the inside of medical devices because it is released in the state, and the sterilization method that irradiates gamma rays can be applied to plastic products, but it is difficult to apply to plastic products and the manufacturing cost becomes expensive because special treatment must be performed to endure gamma rays. There was a problem.

The present invention is to solve the above-mentioned problems of the prior art, an object of the present invention is not only capable of disinfection in a sealed syringe or plasma extraction kit manufactured at the factory, but also when injecting the blood extracted from the patient into the kit It is to provide a plasma extraction kit that is less concerned about contamination and easy to inject and seal.

In addition, another object of the present invention is to provide a plasma extraction kit in which the extracted PRP is increased in purity and concentration, the ease of extraction operation is further improved, and the manufacturing cost can be reduced.

In order to achieve the above object, the plasma extraction kit according to the present invention, the upper end is opened and the lower end is closed with a cylindrical body portion, and fastened to the inner central portion of the main body portion, the diameter of the central portion is small and both ends It characterized in that it comprises a funnel portion formed in a tapered shape that gradually decreases in diameter toward the central portion, and a cover portion for closing the upper end of the main body portion.

In addition, the present invention is the second centrifugal to further extract the plasma by the second centrifugation from the PPP and erythrocytes of the centrifuged blood after the first centrifugation of the blood injected by the plasma extraction kit for extracting the plasma components from the blood Characterized in that comprises a separator tube.

According to the present invention having the above-described configuration, it is possible to easily extract a high-purity high concentration of PRP, can be extracted by almost eliminating red blood cells in the extraction process, the degree of variation is small according to the operator and the extraction time can be shortened By lowering the manufacturing cost of the centrifuge tube, there is an advantage that can reduce the treatment cost of the operators. After centrifugation, a large amount of red blood cells in the blood component is accommodated in the blood storage unit, and a small amount of PRP can be accommodated in the center of the funnel having a small diameter, thereby facilitating extraction of a small amount of PRP.

In addition, even if the amount of red blood cells differs from person to person, since the position of the funnel can be adjusted, the PRP can be accurately positioned in the funnel, thereby easily extracting high-purity PRP with high purity and red blood cells in the extraction process. The extraction can be almost excluded, and the degree of variation according to the operator is small, the extraction time can be shortened, and the manufacturing cost of the centrifuge tube can be lowered to reduce the treatment cost of the operators.

1 is a view showing a plasma extraction kit of the prior art,

2 is a view showing a plasma extraction kit according to a first embodiment of the present invention,

3 is a perspective view and a cross-sectional view of the plasma extraction kit of the first embodiment,

4 is a perspective view and a cross-sectional view of the funnel to be inserted into the plasma extraction kit of the first embodiment,

5 is a cross-sectional view of the blood layer after the first centrifugation,

6 is a cross-sectional view showing the first PRP and PPP extraction;

7 is a cross-sectional view showing the appearance of excess red blood cells after the first centrifugation;

Figure 8 is a cross-sectional view showing the appearance of red blood cells under the first centrifugation,

9 is a perspective view and a cross-sectional view of the secondary centrifuge tube,

10 is a cross-sectional view of the blood layer after secondary centrifugation,

11 is a cross-sectional view showing the extraction of the PPP of the upper layer,

12 is a cross-sectional view showing a small amount of remaining PPP, PRP, small amount of red blood cells,

13 is a cross-sectional view showing a state of extracting the final PRP,

14 is a perspective view showing a plasma extraction kit according to a second embodiment of the present invention,

FIG. 15 is a cutaway perspective view of FIG. 14; FIG.

16 is a cross-sectional view of the plasma extraction kit according to a second embodiment of the present invention.

17 and 18 are views showing the outer cylinder of the plasma extraction kit according to a second embodiment of the present invention,

19 is a view showing the opening and closing cap of the plasma extraction kit according to a second embodiment of the present invention,

20 is a view showing a cover portion of the plasma extraction kit according to a second embodiment of the present invention,

21 is a view showing the connection blade portion of the plasma extraction kit according to a second embodiment of the present invention,

22 is a diagram showing an example of use of the plasma extraction kit according to the second embodiment of the present invention.

Plasma extraction kit according to the present invention, the upper end is opened and the lower end is closed, the main body portion is fastened to the inner central portion of the main body portion, the diameter of the center portion is small and both ends are gradually reduced in diameter toward the center portion A funnel formed in a tapered shape, and a cover portion for closing the upper end of the main body portion.

Here, the inner side of the main body portion is characterized in that the locking step is formed to prevent the separation to maintain the funnel in a predetermined position.

The opening and closing is fastened to the cover portion, characterized in that it comprises an opening and closing cap having an injection hole on one side that can be inserted into the needle of the syringe for injecting blood extracted from the patient.

Here, the opening and closing cap, and closes the opening formed in the center of the upper surface, characterized in that it further comprises a sealing membrane formed of a material that can be easily inserted into the syringe for extracting the multi-platelet plasma of the centrifuged blood.

The cover part has a fastening hole formed at the center thereof, and includes a protrusion projecting from the fastening hole, and the opening / closing cap is provided with a locking protrusion projecting outward from the lower end to be locked to the protrusion of the cover part. .

It is provided on the lower portion of the funnel, characterized in that it further comprises a blood storage unit for receiving the blood is installed is installed in the inner bottom of the main body portion.

The funnel is formed of an inner cylinder formed in a tapered shape in which the diameter of the center portion is small and both ends thereof are tapered in diameter gradually decreasing toward the center portion, and an outer cylinder formed in a hollow cylindrical shape in which upper and lower portions are opened and both ends of the inner cylinder are fastened. It is characterized by.

Here, the outer cylinder is formed with a fastening protrusion protruding outward, the inner surface of the main body portion is indented concave inwardly and has a predetermined width, the fastening protrusion is inserted into the fastening groove for limiting the movement of the fastening protrusion is It is characterized by being formed.

Here, one end is connected to the funnel and the other end is bent and characterized in that it further comprises an elongated rod-shaped connecting blade formed with a fastening projection engaging the thread of the cover portion.

Here, the connection blade portion is characterized in that it comprises a first wing portion formed in a T-shape at one end, and a connecting projection fastened to the fastening screw formed on the cover portion at the other end.

Here, the upper end portion of the outer cylinder is formed by cutting in a T-shape and characterized in that it further comprises a locking groove that is fastened to the first wing portion.

In addition, by the plasma extraction kit for extracting the plasma components from the blood, the second centrifugal centrifugation to further extract the plasma from the PRP, PPP and erythrocytes of the centrifuged blood by centrifugation of the injected blood first Characterized in that further comprises a separation tube.

The secondary centrifuge tube is formed in a cylindrical shape, the lower end of the secondary centrifuge tube is formed with a second capillary tube having a smaller diameter than the upper portion, the tip portion of the second capillary tube is gradually reduced in diameter is formed It is characterized by being.

Hereinafter, with reference to the accompanying drawings will be described in detail with respect to the plasma extraction kit according to the present invention.

2 to 13 is a view showing a plasma extraction kit according to a first embodiment of the present invention.

As shown in Figures 2 to 13, the plasma extraction kit 1 according to the present embodiment is provided as two centrifuge tube cylinders of different shapes, respectively, used as a main body portion, secondary centrifuge tube, each The shape is provided as designed to be easily used in the extraction process.

The inlet of the main body and the secondary centrifuge tube, which will be described later, is closed by lids 40 and 40 ', and the central portion of the lid is formed with perforations 42 and 42' through which a needle can enter. It is configured to inject blood by inserting a needle through the drilling hole without removing the upper cover, the cover is formed of a soft elastic material to be safe from external infection.

The above-described cover has a portion to be inserted into the main body portion, the surface of which is formed with threads 41 and 41 '.

A funnel portion 20 formed in a cylindrical shape is inserted into the main body portion, and a capillary tube 23 having a long narrow diameter penetrating the upper and lower portions of the funnel portion is formed in the center of the funnel portion 20. The upper and lower ends of the capillary are formed with funnel-shaped

tapered portions

21 and 22 that are narrower or wider in diameter, and the main body and the funnel are formed of a transparent material and are located in the inner capillary from the outside. Blood components can be seen clearly. The inserted funnel is provided in close contact with the inner diameter of the main body portion accurately. The inclination of the funnel-shaped taper is designed differently for the upper and lower parts.

In the upper end of the funnel portion, two narrow, long rod-shaped connecting wings 24 extend upward, leading to the cover, the end of which is bent inward to form a fastening protrusion 25 to form a screw thread at the bottom of the cover. It is provided in a state capable of being bitten by 26.

When the cover 40 is rotated, the funnel portion of the lower portion connected to the connecting wing part is changed in height while moving up and down along the thread of the cover. The side of the cover is made of irregularities (43) to increase the friction force during rotation

When the funnel is connected to the cover by a wing, it is floating in the middle without descending to the bottom of the main body. The top and bottom of the cylinder of the portion where the funnel is located is provided with a locking jaw 27 is spaced by a predetermined distance so that the cylindrical structure does not reach the floor or come out of the upper limit.

The lower space of the funnel portion is a space in which red blood cells are collected during centrifugation, and the blood storage unit 30 calculates the diversity of red blood cells of each person and secures a space of appropriate capacity.

The secondary centrifuge tube (2) is formed in a substantially cylindrical shape, the lower end of which is formed a narrow second capillary tube (71) having a smaller diameter than the upper portion, and the end of the second capillary tube (71) is conical. As the diameter becomes smaller, the tip portion 72 is formed which ends at the vertex.

Centrifugation to extract the PRP is usually carried out twice. After the first centrifugation, the ratio of the upper plasma and the lower red blood cells is measured, and the position is adjusted. Then, the second centrifugation is performed. The products developed so far are performed twice in an integrated centrifuge tube. The plasma extraction kit according to the present embodiment is characterized in that the first centrifugation and the second centrifugation are performed in different centrifuge tubes.

As described above, since the amount of human red blood cells is different, the plasma extraction kit 1 of the present embodiment is designed to accommodate the range of this deviation and facilitate extraction.

The plasma extraction kit 1 is divided into an upper space 10, a funnel 20, and a blood storage unit 30 from above. After centrifugation, the upper space 10 is a section where plasma is collected, the blood storage unit 30 is a space where red blood cells are collected, and the funnel 20 is designed to form a boundary of two layers. The upper end 21 and the lower end of the funnel are formed with funnel-shaped

tapered parts

21 and 22 that are gradually smaller or larger in diameter so that the blood cell components can be moved well during centrifugation.

In addition, the plasma extraction kit has a capacity designed so that the red blood cells in the range showing the distribution of the upper and lower 80-90% of the total based on the average red blood cells can be accommodated in the lower space and the narrow and long capillary 23 of the funnel. It is. Therefore, after the first centrifugation, the position of the uppermost layer of red blood cells is located in any part of the capillary. Immediately above, the PRP is densely packed and thickened as the cross-sectional area of the centrifuge tube is reduced. In this case, the needle is inserted directly without the need for height adjustment of the funnel to extract all of the upper PPP including the PRP at once.

At this time, at the boundary between the upper surface of the red blood cells and the lower surface of the PRP, two types of blood cells are entangled with each other. In the step of extracting this part, it takes a long time to extract the red blood cells as much as possible. Depending on the skill, the quality of the extract will vary. In order not to inhale erythrocytes, a significant amount of platelet extraction, which is entangled with erythrocytes at the interface, must be given up, and if you want to increase the extraction rate of platelets, you have no choice but to inhale erythrocytes together. However, in this device, the inhaled red blood cells can be excluded through the second process, so all the PRP and PPP can be sucked quickly together with the PRP at the boundary including the upper red blood cell layer. Ends.

As shown in Fig. 7 and 8, after the first centrifugation, if the elongated capillaries are not accommodated due to excessive red blood cells or excessive red blood cells, the cover 41 is turned to turn the height of the inner tube. By adjusting the PRP layer is located in any part of the capillary and PRP and PPP are extracted in the manner described above.

At this time, the length of the capillary tube is very long, so even if the position adjustment is a little, the position of the PRP falls within the acceptance range of the capillary tube, which is much easier to extract than the narrow capillary product.

The extracted PPP, PRP and a small amount of sucked red blood cells are transferred to the secondary centrifuge tube (2) and subjected to secondary centrifugation.

As shown in FIG. 9, the secondary centrifuge tube 2 is formed in a substantially cylindrical shape, and at the lower end thereof, a narrow second capillary tube 71 having a smaller diameter than the upper portion is formed, and the second capillary tube 71 is formed. At the end of the tip) is a conical shape is made smaller in diameter and ends are formed with a tip portion 72 ends with a vertex.

Centrifugation with this specially designed secondary centrifuge tube forms PPP, PRP, and a small amount of red blood cells inhaled from the top.

Since the upper PPP 81 is not necessary, only a small amount is extracted and discarded.

All that remains is a small amount of PPP 82, PRP 83, and a small amount of red blood cells 84 from above.

The lowest red blood cells are all contained within the tip portion 72 of the secondary centrifuge tube.

Now, the needle touches the PRP layer and extracts the remaining liquid except the lower red blood cells, and since the lower red blood cells are collected only in a very narrow space, it is possible to extract only a small amount of PPP and PRP with very little touch without extracting them. High concentration PRP can be obtained.

Another method is to first extract the red blood cells and discard them by inserting the needles into the red blood cell layer of the uppermost space of the bottom layer, and then extracting and discarding the upper layer of PPP, leaving only a small amount of PPP and PRP. This ensures high purity and high concentration of PRP treatment solution by extracting all the remaining solution in the cylinder all at once without the need for timely extraction.

Either way, it is very easy to exclude red blood cells and extract PRP. The extracted PRP becomes high concentration pure PRP with few red blood cells.

Another advantage of using this device is the ability to control the concentration of PRP. When extracting the upper PPP, the concentration of PRP changes according to the amount of PPP remaining. The number of platelets in a normal person is about 100,000 to 200,000 per microliter, but the concentration should be concentrated to more than 1 million, which is five times that of PRP.

If only PRP is extracted after almost removing without leaving PPP, the number of platelets can reach 1.6 million to 1.8 million. However, in this case, the final treatment solution composed of PRP is too small, so the treatment area is limited to a very narrow area. .

For this reason, PRP is extracted including a small amount of PPP in order to secure an appropriate volume of PRP injection. If you want to extract different concentration rates, adjust the amount of PPP remaining when extracting PPP in the upper layer after the second centrifugation. Just do it. If you leave a lot of PPP, the dose of the final treatment injection will increase, but it will be a low concentration of PRP. If you leave less PPP, you will get a high concentration of PRP, although the dose of treatment injection is small.

The question of how much the final treatment dose is to be determined is determined by the treating physician according to the area of the treatment site. The use of this device has the advantage that the concentration control can be very simple.

Both the main body and the secondary centrifuge tube are made of a closed type with no connecting part, so it is also characterized by a robust structure without fear of blood leakage by high-speed rotation.

Extracting PRP using the plasma extraction kit according to the present embodiment has the following advantages.

First, the capillary is tapered up and down, and the central part is elongated to accommodate most of the variation due to the change in red blood cell volume, so that no time is required to extract PRP.

Second, when the change in the red blood cell volume is out of the deviation and the PRP exceeds the receiving range of the long capillary tube, there is a secondary correction device that can be extracted by adjusting the height by turning the cylinder cover. At this time, since the capillary is long, there is an advantage that the operator's operating range is wide and easy to extract.

Third, a small amount of erythrocytes included in the extraction of PRP and PPP after the first centrifugation can be excluded through the second centrifuge tube, so that PRP can be extracted without high erythrocytes.

The fourth designed second centrifuge tube can be used to control the concentration of PRP as described above.

Fifth, the main body and the second centrifuge tube are designed to be operated without opening the lid, which is safe against external infection.

Sixth centrifugation, the lower part of the centrifuge tube, which has the highest escape pressure during the centrifugation, is a sealed type with no connection, so there is no risk of blood leakage

The seventh lower part is simpler in structure compared to the existing blood separation device with a height adjusting screw, and thus can be manufactured at a low cost.

14 to 22 are diagrams showing the plasma extraction kit 100 according to the second embodiment of the present invention.

As shown in Figures 14 to 22, the plasma extraction kit according to the present embodiment is the main body 110, the cover 120, the blood storage 130, the

funnel

140, 141, Connection wing unit 150, and includes an opening and closing cap 160.

The main body 110 is formed in a cylindrical shape with an upper end opened and a lower end closed. The material of the main body 110 is not particularly limited, but in the present embodiment, the material is made of a plastic material having low manufacturing cost and easy manufacturing.

A fastening groove 110b is formed at the center of the inner surface of the main body 10. The fastening groove 110b is formed to be concavely cut away from the inner side of the main body, and is formed to have a constant width without being formed over the entire circumference of the inner side of the main body.

By forming the fastening groove 110b, the funnel portion to be described later is placed on the stepped portion 110a formed at the bottom of the fastening groove 110b, and the rotation of the funnel portion by the movement of the connection blade to be described later is performed by the fastening groove. It is configured to be limited by the width.

An upper portion of the main body portion is provided to seal the main body portion with a sealing member 110c such as a packing or an O-ring interposed between the main body portion and a cover portion described later.

The blood storage unit 130 is accommodated in the inner lower end of the main body. The blood reservoir is formed in a cylindrical shape with an upper end opened and a lower end closed, and is hermetically received inside the main body. The blood storage unit 130 receives the red blood cell portion of the blood after centrifugation of the injected blood.

Funnels

140 and 141 are installed above the blood storage unit in the main body. The

funnels

140 and 141 are fastened to the inner central portion of the main body, and as shown in FIGS. 15 and 16, the diameter of the central portion is small and both ends are formed in a tapered shape in which the diameter gradually decreases toward the central portion.

As a result, red blood cells and platelets are intertwined in the boundary area between the upper surface of the red blood cells and the lower surface of the PRP. In the step of extracting these parts, it takes a long time to extract the red blood cells as much as possible. According to the present invention, the quality of the extract may vary, but according to the present invention, after centrifugation, a large amount of red blood cells in the blood component is accommodated in the blood storage unit, and a small amount of PRP can be accommodated in the center of the funnel having a small diameter. Even if a small amount of PRP is accommodated in the central portion of the funnel having a small diameter, it is possible to easily extract high-purity high concentrations of PRP, and almost eliminate red blood cells in the extraction process.

In the present embodiment, the funnel portion may be formed as one unit, but in the case of injection molding of plastic material, the inner portion of the central portion having a small diameter may cause a defect such as the generation of bubbles due to the occurrence of a defect. For example, it is formed separately from the outer cylinder 140.

The inner cylinder 141 is formed in a tapered shape in which the diameter of the central portion is small and both ends gradually decrease in diameter toward the central portion.

The outer cylinder 140 is formed in a hollow cylindrical shape of the upper and lower openings, the lower end of the outer cylinder is formed to extend inward to protrude, the seating groove 140b is provided on the upper end of the protruding portion. The lower end of the tapered shape of the inner cylinder is inserted and seated in the seating groove 140b so that the inner cylinder and the outer cylinder are integrally connected to each other.

The outer cylinder 140 has a fastening protrusion 140a protruding outward from the outer surface of the outer cylinder along the longitudinal direction of the outer cylinder. The fastening protrusion 140a is inserted into the fastening groove 110b of the main body.

In addition, the upper end of the outer cylinder 140, the locking groove 140c is formed. The locking fixing groove 140c is formed by cutting in an inverted T shape from the upper end of the outer cylinder. The locking blade groove (140c) is inserted so that the first wing portion of the connecting blade portion to be described later is configured so that the connecting blade portion and the outer cylinder behave integrally.

By the above-described configuration, when the connection blade is connected to the upper end of the outer cylinder, and the connection blade is screwed to the cover portion as described below, when the cover portion rotates, the connection blade portion and the outer cylinder connected thereto are rotated. At this time, the fastening protrusion of the outer cylinder is rotated by the width of the fastening groove in the state inserted into the fastening groove of the main body portion is blocked by the end of the fastening groove, thereby the outer cylinder is stopped rotation is the connection blade It is configured to move the position from the inside of the main body portion to the upper or lower by the rotation of the cover portion in a fixed state.

Thus, even if the amount of red blood cells differs from person to person, since the position of the funnel can be adjusted by the configuration of the connecting wing part and the funnel part, the PRP can be accurately positioned in the funnel part, whereby a small amount of PRP has a diameter. It can be accommodated in the center of a small funnel, and even if a small amount of PRP is accommodated in the center of a small funnel, it can easily extract high-purity PRP with high purity and almost eliminate red blood cells in the extraction process. .

The connection wing 150 is one side is fastened to the cover portion, the other side is fastened to the outer cylinder.

The connecting wing unit 150, the first wing portion 152 is formed in a portion that is fastened with the outer cylinder, the connection protrusion 151 is formed on the side that is fastened to the cover portion.

The first wing portion 152 protrudes outward from the width of the connecting wing portion to have a T-shape, and is inserted into the locking fixing groove 140c. The connecting protrusion 151 is formed to protrude a predetermined length toward the inner side, that is, the central side of the main body portion from the main body of the connecting wing portion, is configured to be fastened to the screw thread formed in the cover portion.

The cover part 120 is provided at an upper portion of the main body part, and the cover part 120 is configured to close the upper end of the main body part.

The cover part 120 includes an upper surface covering an upper end of the main body and an extension part extending downward from the upper surface and inserted into the main body, and a thread 121 is formed on an outer circumferential surface of the extension. The screw thread is configured such that the connecting projection of the connecting blade portion is screwed.

A support protrusion 122 is formed between the upper surface of the cover portion and the extension portion, so that the sealing member 110c is installed between the lower portion of the upper surface and the support protrusion to stabilize the cover portion and the main body portion. It is configured to be sealed by.

A fastening hole 123 is formed in the center of the upper surface of the cover part, and the opening and closing cap 160, which will be described later, is inserted into the fastening hole 123 to be opened and closed. In addition, the cover portion is formed with a protrusion 124 protruding toward the center of the fastening hole. The protrusion of the opening / closing cap to be described later is locked to the protrusion, and the opening / closing cap is configured to be opened and closed stably without being separated from the cover part when the opening / closing cap is opened or closed.

The opening and closing cap 160 is inserted into the fastening hole 123 of the cover part. The opening and closing cap 160 is fastened to the cover portion to be openable.

The opening / closing cap 160 includes an injection hole 161, locking

protrusions

162a and 162b, and a sealing membrane 163. The injection hole 161 is formed on one side of the main body of the opening and closing cap so that the needle of the syringe for injecting blood extracted from the patient can be inserted.

The locking protrusion is composed of an upper locking protrusion 162b and a lower locking protrusion 162a and protrudes outward from the main body of the opening / closing cap. When the opening and closing cap is opened, the lower locking protrusion 162a comes into contact with the lower surface of the protruding portion of the cover part, and when the closing cap is closed, the upper locking protrusion 162b is upper surface of the protruding portion. Abuts against and seals between the lid and the opening / closing cap.

The sealing membrane 163 closes the opening formed at the center of the upper surface, and is preferably formed of a material into which a syringe for extracting the multiplatelet plasma of the centrifuged blood can be easily inserted.

An example of using the plasma extraction kit of the present invention having the above-described configuration will be described with reference to FIG.

First, the plasma extraction kit according to the present invention manufactured in a factory is sealed in a wrapping paper with the opening and closing cap open from the cover part. Here, the wrapping paper is preferably made of a special paper material that one side can penetrate the disinfection gas.

When the plasma extraction kit is sterilized using a disinfection gas such as EO gas in the sealed state, the disinfection gas penetrating the packaging is injected into the plasma extraction kit through the injection hole of the opening and closing cap to facilitate disinfection. It is done.

Then, after use, the wrapping paper is peeled off, and the needle of the syringe containing the blood extracted from the patient is injected through the injection hole of the opening / closing cap.

When blood injection is completed, pressing the opening and closing cap seals the lid and the opening and closing cap to complete preparation for plasma extraction.

Then, the plasma extraction kit is centrifuged by a centrifuge, and a plasma collection syringe is injected through the sealing membrane of the opening / closing cap. Then, the end of the needle is inserted into the center of the small diameter of the funnel to insert the PRP. It can be extracted simply.

Thus, by installing an opening and closing cap that can be opened and closed on the cover portion and the injection hole in the opening and closing cap, even if the plasma extraction kit is sealed with a wrapping paper in the state that the cover portion is fastened to the EO gas When disinfecting by the disinfection gas can be introduced into the plasma extraction kit through the injection hole of the opening and closing cap can facilitate disinfection.

In addition, since the opening and closing the cap to open and then insert the needle of the syringe into the injection hole of the opening and closing cap to inject the blood and then press the opening and closing cap to seal, so after removing the entire stopper, the blood is inserted and the stopper Compared with the conventional way of resealing, there is an advantage that there is less concern about contamination and very easy to use.

Claims

A cylindrical body having an upper end opened and a lower end closed;

A funnel portion fastened to an inner central portion of the main body portion, the diameter of the central portion being small and both ends formed in a tapered shape gradually decreasing in diameter toward the central portion;

Plasma extraction kit comprising a cover portion for closing the upper end of the main body portion.
The method of claim 1,

Plasma extraction kit, characterized in that the inner side of the main body portion is formed with a locking jaw to prevent separation to maintain the funnel in a predetermined position.
The method of claim 1,

Plasma extraction kit is fastened to the opening and closing portion, characterized in that it has an opening and closing cap having an injection hole in one side that can be inserted into the needle of the syringe for injecting blood extracted from the patient.
The method of claim 3, wherein

The opening and closing cap, the opening is formed in the center of the upper surface, the plasma extraction kit further comprises a sealing membrane formed of a material that can be easily inserted into the syringe for extracting the multi-platelet plasma of the centrifuged blood .
The method of claim 3, wherein

The cover part has a fastening hole formed in the center, and has a protrusion projecting from the fastening hole,

The opening and closing cap is a plasma extraction kit characterized in that it comprises a locking projection protruding outward from the lower end is locked to the protrusion of the cover portion.
The method of claim 1,

Plasma extraction kit is provided in the lower portion of the funnel and further provided with a blood storage unit for receiving the blood is installed is installed in the inner bottom of the main body portion.
The method of claim 1,

The funnel,

Inner passage formed in a tapered shape in which the diameter of the central portion is small and both ends gradually decrease in diameter toward the central portion,

Plasma extraction kit characterized in that the upper and lower portions are formed in an outer cylinder formed in a hollow cylindrical shape to which both ends of the inner cylinder is fastened.
The method of claim 7, wherein

The outer cylinder has a fastening protrusion protruding outward,

Plasma extraction kit characterized in that the inner surface of the main body portion is indented concave and has a predetermined width is inserted into the fastening protrusion and restricting the movement of the fastening projection.
The method of claim 7, wherein

One end is connected to the funnel portion and the other end is bent plasma extraction kit further comprises a long rod-shaped connecting blade formed with a fastening projection engaging the thread of the cover portion.
The method of claim 9,

The connecting wing portion,

A first wing portion formed in a T shape at one end,

Plasma extraction kit characterized in that it comprises a connecting projection which is fastened to the fastening screw formed on the cover portion at the other end.
The method of claim 10,

Plasma extraction kit, characterized in that the upper end portion of the outer cylinder is formed by cutting in a T-shape and further comprising a locking fixing groove to which the first wing is fastened.
The method of claim 1,

Secondary centrifuge tube which further extracts plasma by second centrifugation from PRP, PPP and erythrocytes of centrifuged blood after centrifuging the injected blood first by plasma extraction kit which extracts plasma components from blood. Plasma extraction kit, characterized in that further comprises a.
The method of claim 12,

The secondary centrifuge tube is formed in a cylindrical shape,

A second capillary tube having a diameter smaller than an upper portion at a lower end of the secondary centrifuge tube,

Plasma extraction kit, characterized in that the tip portion of the second capillary tube is gradually reduced in diameter is formed.