WO2010127503A1

WO2010127503A1 - Device and method for detecting urinary system tuberculum using dna genetic information

Info

Publication number: WO2010127503A1
Application number: PCT/CN2009/071697
Authority: WO
Inventors: 蔡志明; 桂耀庭; 唐爱发
Original assignee: Cai Zhiming; Gui Yaoting; Tang Aifa
Priority date: 2009-05-08
Filing date: 2009-05-08
Publication date: 2010-11-11

Abstract

A device and method for detecting a urinary system tuberculum using the DNA genetic information are disclosed, and the device includes at least a reaction module pre-set a urinary system tuberculum DNA database sequence information, a signal reaction detecting module and a processor module pre-set by the status information, the reaction module, the signal reaction detecting module, and the processor module are connected, the reaction module is used for reacting with the DNA sample to be detected, the signal reaction detecting module detects the status information of the reaction, and feeds back the status information to the processor module, the processor module processes an analysis according to the feedback status information and the pre-set status information. By the device and the method, the high flux automatic detection for the urinary system tuberculum is realized, and the pathology information of the patient to be inspected can be estimated and prognosticated early.

Description

Device and method for detecting urinary system tuberculosis by using DNA genetic information

[1] The present invention relates to an apparatus and method for detecting urinary system tuberculosis.

Background technique

[2] Tuberculosis can occur in the genitourinary system from the kidneys, ureters, bladder to the urethra; the most common and first occurring is the kidney. The lesion can spread downwards and spread to the male reproductive system. Urogenital tuberculosis mainly refers to kidney tuberculosis; tuberculosis lesions that are usually secondary to other parts, such as tuberculosis, spread, or from bone and joint tuberculosis, intestinal tuberculosis. About 1 to 4% of tuberculosis patients have clinical genitourinary tuberculosis, and tuberculosis patients have urinary tuberculosis test, 7 to 8% are positive, including clinical renal tuberculosis and partial pathological renal tuberculosis. For patients who died of tuberculosis, autopsy found that 96% had pulmonary tuberculosis, 26% had genitourinary tuberculosis, and patients who died of tuberculosis without clinical tuberculosis showed continuous renal biopsy. There are tuberculous lesions and healing scars in the kidney, and the lesions are mainly in the cortex of the kidney. Urogenital tuberculosis is more common in men, with 20 to 40 years old being common (66.3%). About 50 to 70% of male renal tuberculosis patients develop tuberculosis of the reproductive system. The more severe the genitourinary tuberculosis, the more opportunities there are for male reproductive system tuberculosis. The pathogen of tuberculosis of genitourinary tuberculosis spreads to the kidneys. First, miliary tuberculosis nodules occur in multiple glomeruli of the kidneys, and caseous necrosis can occur in the center of the nodules. Disclosure of invention

technical problem

[3] The technical problem to be solved by the present invention is to overcome the deficiencies of the prior art and to provide a detecting device and method capable of performing high-throughput automatic detection of urinary system tuberculosis.

Technical solution

[4] The technical solution adopted by the present invention to solve the technical problem thereof is: a device for detecting urinary system tuberculosis by using DNA genetic information, comprising a reaction module at least preset with urinary system tuberculosis DNA library sequence information, and a signal reaction detecting module And a processor module preset with state information, a reaction module, a signal reaction detection module, a processor module connection, a reaction module for reacting with the DNA sample to be tested, a signal reaction detection module detecting the state information of the reaction, and the state information Feedback to the processor module, the processor module is based on The feedback status information and preset status information are analyzed.

[5] The reaction module includes a DNA probe containing sequence information of the urinary tuberculosis DNA library and a DNA probe containing control sequence information. The reaction module includes micropores (e.g., nanotubes and microtubules) having coordinates, and the microwells correspond one-to-one with the DNA probes. The reaction module further includes a reaction system including a DN

A detects the reaction solution and the washing solution. The signal reaction detection module is further connected with a sensor module. The reaction module is a DNA microarray chip comprising a DNA probe containing urinary tract tuberculosis DNA library sequence information and a DNA probe containing control sequence information.

[6] The signal reaction detection module includes an image sensor for acquiring an image of a reaction module. The processor module is further connected with a display module and an interface module.

[7] A device for detecting urinary tuberculosis using DNA genetic information, including pre-set urinary tuberculosis D

The reaction module of the NA library sequence information and the control sequence information, the signal reaction detection module and the processor module preset with the state information, the reaction module, the signal reaction detection module and the processor module are sequentially connected, and the reaction module is used for the DNA to be tested. The sample reaction, the signal reaction detection module detects the status information of the reaction, and feeds the status information to the processor module, and the processor module performs analysis according to the feedback status information and the preset status information. The reaction module has a plurality of coordinate points, each of which is provided with a DNA probe containing urinary system TB DNA library sequence information or a DNA probe containing control sequence information. The reaction module has a plurality of micropores, each micropore corresponding to one coordinate, and each micropore has a DNA containing urinary system tuberculosis DNA library sequence information or DNA containing control sequence information.

[8] A method for detecting urinary tuberculosis by using DNA genetic information, comprising the steps of: a) distributing a sample of DNA to be tested on a surface of a DNA microarray chip, and reacting with DNA in a preset DNA probe or microwell; b) Obtain DNA samples and DNA to be tested

Status information of the probe reaction; c) analysis based on the acquired status information and preset status information. In the step c), the judgment result is obtained by comparing the acquired state information with the preset state information, and the state information is selected from the group consisting of: color, brightness, current on and off, level.

Beneficial effect

[9] The beneficial effects of the invention are: the reaction module reacts with the DNA sample to be tested, the signal reaction detection module detects the reaction state, the processor module compares the state information and obtains the analysis result, thereby achieving high urinary system tuberculosis Flux automated detection, early assessment and prediction of pathological information of patients to be tested Interest.

DRAWINGS

[10] FIG. 1 is a device for detecting urinary system tuberculosis using DNA genetic information in the present embodiment.

FIG. 2 is a schematic structural view of a DNA microarray chip of the present embodiment.

Embodiments of the invention

[11] As shown in FIG. 1 and FIG. 2, the apparatus for detecting urinary tuberculosis using DNA genetic information in the present embodiment includes a reaction module, a sensor module, a signal reaction detecting module, a processor module, a display module, and an interface module. The reaction module and the sensor module are all connected to the signal reaction detection module; the signal reaction detection module is connected to the processor module; the display module and the interface module are all connected to the processor module. The reaction module is preset with urinary tuberculosis DNA library sequence information and control sequence information. The sample to be tested is evenly distributed on the surface of the reaction module, and the signal reaction detection module detects the reaction state of the reaction module and the sample to be tested and the feedback information of the sensor module, and is sent to the processor module after preprocessing. The processor module processes information, analyzes, and sends the calculated result value to the display module for display or to the interface module for output.

[12] The sample to be tested is taken from the blood or other tissues of the object to be tested (such as hair follicles, etc.), and after special treatment, a DNA sample with better detection characteristics is obtained, and the special treatment is coupled with a fluorescent dye as in the prior art.

[13] The reaction module comprises a reaction system, at least one DNA probe containing sequence information of the urinary tuberculosis DNA library, and at least one DNA probe containing control sequence information, and each urinary system tuberculosis DNA library sequence information corresponds to a control sequence information, The urinary system tuberculosis DNA library sequence information such as the characteristic DNA library sequence information of pathogens such as Mycobacterium tuberculosis. Each DNA probe corresponds to a coordinate and the DNA probe can be coupled to fluorescein, biotin or other label. The reaction system includes a DNA detection reaction solution and a wash solution.

[14] The signal response detection module includes an image sensor with a microscope head (eg CCD, CMOS)

Sensor), after pre-processing (such as photoelectric conversion, AD analog-to-digital conversion), input the processor module, and pre-process the state value of the reaction system state, such as temperature, special ion and humidity, which are fed back by the sensor module. After transmission to the processor module.

[15] The sensor module monitors the temperature, specific ions, humidity, etc. of the reaction system of the reaction module.

[16] The processor module can be an embedded or PC hardware and software system architecture, which can analyze and process the information transmitted by the signal reaction detection module and the sensor module. The processor module can be built-in image recognition and processing software module, and complete the coordinate confirmation, brightness recognition, color recognition, or inspection of the image information of the reaction module. Check the current on and off, level to achieve qualitative analysis, if there is brightness, the DNA at the coordinate point can react with the sample to be tested. The DNA sequence consists of four bases, A, T, C, and G, which are paired with 1\C and G. The DNA hybridization reaction is described below as an example. As shown in Figure 2, the DNA sequence of the A-coordinate DNA probe is shown. The DNA sequence of the AATTCG, M coordinate DNA probe is TGCGA. If the A coordinate has brightness, it means there is a complementary sequence (TTAAGC); if the M point has no brightness, it means there is no complementary DNA sequence that reacts with it. (ACGCT). The DNA probe containing the control sequence information of the reaction module is a DNA probe of a normal gene.

[17] The display module is used to display various types of displays such as CRT and LCD LED. The interface module is used to realize the signal connection between the entire detecting device and the outside world, such as various serial communication and network interfaces.

[18] Using the DNA library to detect urinary tuberculosis:

[19] Reference OMIM (Ment Mendelian Inheritance Online, Online Mendelian Inheritance in

Man), select N urinary system tuberculosis DNA library sequence information (characterized DNA sequence information of pathogens such as Mycobacterium tuberculosis), and customize the reaction module with DNA library sequence information and control sequence information.

[20] Taking tissue containing DNA genetic information such as blood or hair follicles to be tested, after pretreatment, making it a DNA sample with good detection characteristics, and fully aligning the DNA sample with the coordinates of the reaction module The DNA probe is hybridized, and after washing to remove the unreacted DNA sample, the reaction signal is sent to the processor module via the signal reaction detection module. The processor module converts the response signal into an interpretable signal for qualitative analysis of the reaction between the DNA library sequence information in the reaction module and the sample to be tested, thereby interpreting the health level of the genitourinary system of the subject to be tested.

[21] In the present embodiment, the DNA library sequence information is characteristic DNA sequence information of a pathogen such as Mycobacterium tuberculosis, and the reaction module includes a DNA probe of a plurality of normal genes as an analysis control, and the reaction between the sample to be tested and the DNA probe is performed. To qualitatively analyze the sample to be tested. The reaction module is a DN A microarray chip having a plurality of DNA probes. After the reaction module reacts with the DNA sample to be tested, the state information (such as color, brightness, gray level, current, level) of the reaction module changes, such as the color of the image before the reaction module reacts, the brightness, and the color of the image after the reaction. Different brightness, current on/off state or level difference; obtain the image of the reaction module and the status information of the reaction module through the signal reaction detection module and feed back to the processor module

The processor module compares the status information with preset status information.

The health of the genitourinary system can be analyzed. Peer, due to each DNA sequence of the reaction module The column has its own coordinates, which can be used to visually determine which gene of the patient is mutated by changing the state information of the coordinate (for example, the DNA probe changes from no brightness to brightness). The reaction module may include only a DNA probe containing information on the sequence of the urinary tuberculosis DNA library, or a DNA probe containing information on the sequence of the urinary tuberculosis DNA library and a DNA probe containing information on the control sequence.

[22] A method for detecting urinary tuberculosis using DNA genetic information, comprising the following steps:

[23] a) uniformly distributing the sample to be tested (which can be coupled with the fluorescent dye) on the surface of the reaction module, and reacting with the DNA in the preset DAA probe or micropore;

[24] b) The signal reaction detection module sends the detected reaction module and the reaction state information of the sample to be tested, and the information fed back by the sensor module (temperature, special ion, humidity, etc.) to the processor module after being preprocessed, the processor The result value calculated by the module is displayed by the display module or output via the interface module.

[25] The above is a further detailed description of the present invention in conjunction with the specific preferred embodiments. It is not intended that the specific embodiments of the invention are limited to the description. It will be apparent to those skilled in the art that the present invention may be practiced without departing from the spirit and scope of the invention.

Claims

Claim

[1] A device for detecting urinary system tuberculosis by using DNA genetic information, comprising: a reaction module including at least a urinary system tuberculosis DNA library sequence information, a signal reaction detecting module, and a processor module preset with state information; The reaction module, the signal reaction detection module and the processor module are connected, the reaction module is configured to react with the DNA sample to be tested, the signal reaction detection module detects the status information of the reaction, and feeds the status information to the processor module, the processor module The analysis is based on the feedback status information and the preset status information.

[2] The apparatus for detecting urinary system tuberculosis by using DNA genetic information according to claim 1, wherein: the reaction module comprises a DNA probe containing sequence information of a urinary system tuberculosis DNA library and a DNA probe containing control sequence information. needle.

[3] The apparatus for detecting urinary tuberculosis using DNA genetic information according to claim 2, wherein the reaction module comprises micropores having coordinates, and the micropores are in one-to-one correspondence with the DNA probes.

[4] The apparatus for detecting urinary system tuberculosis using DNA genetic information according to claim 1, wherein the reaction module further comprises a reaction system comprising a DNA detecting reaction solution and a washing liquid.

[5] The apparatus for detecting urinary system tuberculosis using DNA genetic information according to claim 5, wherein the signal reaction detecting module is further connected with a sensor module for feeding back a state of the reaction system.

[6] The apparatus for detecting urinary system tuberculosis by using DNA genetic information according to claim 1, wherein: the reaction module is a DNA microarray chip, and the DNA microarray chip comprises sequence information of a urinary system tuberculosis DNA library. DNA probes and DNA probes containing control sequence information.

[7] The apparatus for detecting urinary system tuberculosis using DNA genetic information according to any one of claims 1 to 6, wherein the signal reaction detecting module comprises an image sensor for acquiring an image of the reaction module.

[8] The apparatus for detecting urinary system tuberculosis using DNA genetic information according to claim 7, wherein: the processor module is further connected with a display module and an interface module.

[9] A device for detecting urinary tuberculosis by using DNA genetic information, comprising: a reaction module including a urinary system tuberculosis DNA library sequence information and a control sequence information, and a signal reaction test The measuring module and the processor module preset with the state information, the reaction module, the signal reaction detecting module and the processor module are sequentially connected, the reaction module is used for reacting with the DNA sample to be tested, and the signal reaction detecting module detects the state information of the reaction, The status information is fed back to the processor module, and the processor module performs analysis according to the feedback status information and the preset status information.

[10] The apparatus for detecting urinary system tuberculosis using DNA genetic information according to claim 9, wherein: the reaction module further comprises a reaction system including a DNA detection reaction solution and a washing solution, and the signal reaction The detection module is also connected with a sensor module for feeding back the state of the reaction system.

[11] The apparatus for detecting urinary system tuberculosis by using DNA genetic information according to claim 9 or 10, wherein: the reaction module has a plurality of coordinate points, and each coordinate point is provided with a urinary system tuberculosis DNA library sequence Information DNA probe or DNA probe containing control sequence information.

[12] The apparatus for detecting urinary system tuberculosis by using DNA genetic information according to claim 9 or 10, wherein: the reaction module has a plurality of micropores, each micropore corresponding to one coordinate, and each micropore DNA containing information on the sequence of the urinary tuberculosis DNA library or DNA containing the control sequence information is built in.

[13] The apparatus for detecting urinary system tuberculosis using DNA genetic information according to claim 9 or 10, wherein: the signal reaction detecting module includes an image sensor for acquiring an image of a reaction module, and the image sensor acquires a reaction After the image state information, the processor module analyzes the image state information after the reaction and the preset image state information.

[14] A method for detecting urinary tuberculosis by using DNA genetic information, comprising: step a) distributing a sample of DNA to be tested on a surface of a DNA microarray chip, and a preset DNA probe or a micropore DNA reaction;

b) obtaining state information of the reaction of the DNA sample to be tested with the DNA probe or the DNA in the micropore; c) performing analysis according to the acquired state information and preset state information.

[15] The method for detecting urinary system tuberculosis by using DNA genetic information according to claim 14, wherein: in the step c), the judgment result is obtained by comparing the acquired state information with the preset state information. Information is selected from: color, brightness, current on and off, high and low levels. The method for detecting urinary tuberculosis using DNA genetic information according to claim 14 or 15, wherein the DNA on the DNA microarray chip is characteristic DNA of Mycobacterium tuberculosis.