WO2009122159A3 - Biosensor for analyte detection - Google Patents

Biosensor for analyte detection Download PDF

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Publication number
WO2009122159A3
WO2009122159A3 PCT/GB2009/000843 GB2009000843W WO2009122159A3 WO 2009122159 A3 WO2009122159 A3 WO 2009122159A3 GB 2009000843 W GB2009000843 W GB 2009000843W WO 2009122159 A3 WO2009122159 A3 WO 2009122159A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
biosensors
capture electrode
probe
biosensor
Prior art date
Application number
PCT/GB2009/000843
Other languages
French (fr)
Other versions
WO2009122159A2 (en
Inventor
Piero Migliorato
Peng Li
Pedro Estrela
Simon Keighley
Original Assignee
Cambridge Enterprise Limited
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Enterprise Limited filed Critical Cambridge Enterprise Limited
Publication of WO2009122159A2 publication Critical patent/WO2009122159A2/en
Publication of WO2009122159A3 publication Critical patent/WO2009122159A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • G01N33/5438—Electrodes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

This invention relates to biosensors for detecting analyte molecules, such as nucleic acids, in a sample solution. The biosensors comprise a capture electrode which has probe molecules immobilised at its surface which specifically bind to analyte molecules. The probe molecules are separated on the surface of the capture electrode by spacer molecules and the proportion of probe molecules to spacer molecules is less than 10%. Changes in the electrical properties of the capture electrode surface are indicative of the binding of the immobilised probe molecules to analyte molecules. Biosensors as described may be useful for example, in diagnostic, genomic and forensic applications.
PCT/GB2009/000843 2008-03-31 2009-03-31 Biosensor for analyte detection WO2009122159A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB0805814A GB0805814D0 (en) 2008-03-31 2008-03-31 Biosensor for analyte detection
GB0805814.1 2008-03-31

Publications (2)

Publication Number Publication Date
WO2009122159A2 WO2009122159A2 (en) 2009-10-08
WO2009122159A3 true WO2009122159A3 (en) 2009-12-10

Family

ID=39387044

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2009/000843 WO2009122159A2 (en) 2008-03-31 2009-03-31 Biosensor for analyte detection

Country Status (2)

Country Link
GB (1) GB0805814D0 (en)
WO (1) WO2009122159A2 (en)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130240376A1 (en) 2010-09-02 2013-09-19 Heed Diagnostics Aps Electrochemical detection of analyte
KR101963462B1 (en) 2010-10-04 2019-03-28 제납시스 인크. Systems and methods for automated reusable parallel biological reactions
US9184099B2 (en) 2010-10-04 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Biosensor devices, systems and methods therefor
US9399217B2 (en) 2010-10-04 2016-07-26 Genapsys, Inc. Chamber free nanoreactor system
US8585973B2 (en) 2011-05-27 2013-11-19 The Board Of Trustees Of The Leland Stanford Junior University Nano-sensor array
US9926596B2 (en) 2011-05-27 2018-03-27 Genapsys, Inc. Systems and methods for genetic and biological analysis
GB201120118D0 (en) * 2011-11-22 2012-01-04 Iti Scotland Ltd Detecting analytes
CN106591103B (en) 2011-12-01 2021-06-04 吉纳普赛斯股份有限公司 System and method for efficient electronic sequencing and detection
WO2014152625A1 (en) * 2013-03-15 2014-09-25 Genapsys, Inc. Systems and methods for biological analysis
US10125393B2 (en) 2013-12-11 2018-11-13 Genapsys, Inc. Systems and methods for biological analysis and computation
US9822401B2 (en) 2014-04-18 2017-11-21 Genapsys, Inc. Methods and systems for nucleic acid amplification
CN116397014A (en) 2016-07-20 2023-07-07 测序健康公司 Systems and methods for nucleic acid sequencing
JP7339245B2 (en) * 2017-06-12 2023-09-05 エッセンリックス コーポレーション Homogeneous assay method
EP3684951A4 (en) 2017-09-21 2021-06-16 Genapsys, Inc. Systems and methods for nucleic acid sequencing
WO2023092210A1 (en) * 2021-11-26 2023-06-01 Universidade Estadual Paulista Julio De Mesquita Filho Method and device for amplifying transducer signals for capacitive assays

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
AOKI HIROSHI; BUHLMANN PHILIPPE; UMEZAWA YOSHIO: "Electrochemical detection of a one-base mismatch in an oligonucleotide using ion-channel sensors with self-assembled PNA monolayers", November 2000, ELECTROANALYSIS, VOL. 12, NR. 16, PAGE(S) 1272-1276, ISSN: 1040-0397, XP002548842 *
AOKI HIROSHI; UMEZAWA YOSHIO: "Ion-channel sensors for electrochemical detection of DNA based on self-assembled PNA monolayers.", 2002, NUCLEIC ACIDS RESEARCH. SUPPLEMENT (2001) 2002, NR. 2, PAGE(S) 131 - 132, XP002548841 *
CHEN D ET AL: "Interfacial design and functionization on metal electrodes through self-assembled monolayers", SURFACE SCIENCE REPORTS, ELSEVIER SCIENCE, NL, vol. 61, no. 11, 1 December 2006 (2006-12-01), pages 445 - 463, XP025035664, ISSN: 0167-5729, [retrieved on 20061201] *
KEIGHLEY ET AL: "Optimization of DNA immobilization on gold electrodes for label-free detection by electrochemical impedance spectroscopy", BIOSENSORS AND BIOELECTRONICS, ELSEVIER BV, NL, vol. 23, no. 8, 4 December 2007 (2007-12-04), pages 1291 - 1297, XP022500444, ISSN: 0956-5663 *
KEIGHLEY S D ET AL: "Optimization of label-free DNA detection with electrochemical impedance spectroscopy using PNA probes", BIOSENSORS AND BIOELECTRONICS, ELSEVIER BV, NL, vol. 24, no. 4, 1 December 2008 (2008-12-01), pages 906 - 911, XP025504442, ISSN: 0956-5663, [retrieved on 20080805] *
LIU JIANYUN; TIAN SHENGJUN; NIELSEN PETER E; KNOLL WOLFGANG: "In situ hybridization of PNA/DNA studied label-free by electrochemical impedance spectroscopy.", 21 June 2005, CHEMICAL COMMUNICATIONS (CAMBRIDGE, ENGLAND) 21 JUN 2005, NR. 23, PAGE(S) 2969 - 2971, ISSN: 1359-7345, XP002548840 *

Also Published As

Publication number Publication date
WO2009122159A2 (en) 2009-10-08
GB0805814D0 (en) 2008-04-30

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