WO2009119971A3 - Method for electrically detecting physiologically active materials and biochip for the same - Google Patents

Method for electrically detecting physiologically active materials and biochip for the same Download PDF

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Publication number
WO2009119971A3
WO2009119971A3 PCT/KR2009/000285 KR2009000285W WO2009119971A3 WO 2009119971 A3 WO2009119971 A3 WO 2009119971A3 KR 2009000285 W KR2009000285 W KR 2009000285W WO 2009119971 A3 WO2009119971 A3 WO 2009119971A3
Authority
WO
WIPO (PCT)
Prior art keywords
reaction
active materials
physiologically active
biochip
same
Prior art date
Application number
PCT/KR2009/000285
Other languages
French (fr)
Korean (ko)
Other versions
WO2009119971A2 (en
Inventor
이재훈
Original Assignee
디지탈 지노믹스(주)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 디지탈 지노믹스(주) filed Critical 디지탈 지노믹스(주)
Priority to JP2010549557A priority Critical patent/JP5371061B2/en
Priority to EP09724909A priority patent/EP2273263B1/en
Priority to US12/922,934 priority patent/US8354307B2/en
Priority to CN2009801108754A priority patent/CN101981445B/en
Publication of WO2009119971A2 publication Critical patent/WO2009119971A2/en
Publication of WO2009119971A3 publication Critical patent/WO2009119971A3/en
Priority to US13/708,236 priority patent/US9005545B2/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28—Electrolytic cell components
    • G01N27/30—Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3276—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a hybridisation with immobilised receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/11—Automated chemical analysis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
    • Y10T436/2575—Volumetric liquid transfer

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a method for detecting the existence and/or the reaction of physiologically active materials by accurately detecting changes in the electrical property resulting from biological, biochemical or chemical reaction of physiologically active materials, and a biochip provided for the same. The reaction of the sample for detection is carried out in a general reactive solution, and a reaction chamber is filled with a reference fluid having a large dielectric constant, such as water, prior to reaction for measuring the impedance value or capacitance value. The reactive solution is removed after completion of the reaction, and the reaction chamber is refilled with the reference fluid having a large dielectric constant for measuring the impedance value or capacitance value. Subsequently, the impedance value or capacitance value before or after the reaction is compared to check the existence and/or the reaction of physiologically reactive materials in the sample for detection.
PCT/KR2009/000285 2008-03-24 2009-01-20 Method for electrically detecting physiologically active materials and biochip for the same WO2009119971A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP2010549557A JP5371061B2 (en) 2008-03-24 2009-01-20 Method for electrically detecting physiologically active substance and biochip for the same
EP09724909A EP2273263B1 (en) 2008-03-24 2009-01-20 Method for electrically detecting physiologically active materials and biochip for the same
US12/922,934 US8354307B2 (en) 2008-03-24 2009-01-20 Method for detecting biomolecules electrically and biochip therefor
CN2009801108754A CN101981445B (en) 2008-03-24 2009-01-20 Method for electrically detecting physiologically active materials and biochip for the same
US13/708,236 US9005545B2 (en) 2008-03-24 2012-12-07 Method for detecting biomolecules electrically and biochip therefor

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0027099 2008-03-24
KR1020080027099A KR100969667B1 (en) 2008-03-24 2008-03-24 Method for detecting biomolecules electrically and biochip provided with therefor

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/922,934 A-371-Of-International US8354307B2 (en) 2008-03-24 2009-01-20 Method for detecting biomolecules electrically and biochip therefor
US13/708,236 Division US9005545B2 (en) 2008-03-24 2012-12-07 Method for detecting biomolecules electrically and biochip therefor

Publications (2)

Publication Number Publication Date
WO2009119971A2 WO2009119971A2 (en) 2009-10-01
WO2009119971A3 true WO2009119971A3 (en) 2009-11-19

Family

ID=41114429

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/000285 WO2009119971A2 (en) 2008-03-24 2009-01-20 Method for electrically detecting physiologically active materials and biochip for the same

Country Status (6)

Country Link
US (2) US8354307B2 (en)
EP (1) EP2273263B1 (en)
JP (1) JP5371061B2 (en)
KR (1) KR100969667B1 (en)
CN (1) CN101981445B (en)
WO (1) WO2009119971A2 (en)

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US10254204B2 (en) 2011-03-07 2019-04-09 Accelerate Diagnostics, Inc. Membrane-assisted purification
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Also Published As

Publication number Publication date
WO2009119971A2 (en) 2009-10-01
US8354307B2 (en) 2013-01-15
EP2273263A2 (en) 2011-01-12
KR20090101764A (en) 2009-09-29
US20130143775A1 (en) 2013-06-06
US9005545B2 (en) 2015-04-14
US20110024309A1 (en) 2011-02-03
KR100969667B1 (en) 2010-07-14
JP5371061B2 (en) 2013-12-18
CN101981445A (en) 2011-02-23
CN101981445B (en) 2013-09-04
EP2273263A4 (en) 2011-05-11
EP2273263B1 (en) 2013-03-20
JP2011517769A (en) 2011-06-16

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