WO2009058867A3 - Affinity measurements using frameless multiplexed microarrays - Google Patents
Affinity measurements using frameless multiplexed microarrays Download PDFInfo
- Publication number
- WO2009058867A3 WO2009058867A3 PCT/US2008/081587 US2008081587W WO2009058867A3 WO 2009058867 A3 WO2009058867 A3 WO 2009058867A3 US 2008081587 W US2008081587 W US 2008081587W WO 2009058867 A3 WO2009058867 A3 WO 2009058867A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- relates
- present
- frameless
- affinity measurements
- detection
- Prior art date
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00603—Making arrays on substantially continuous surfaces
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00623—Immobilisation or binding
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00603—Making arrays on substantially continuous surfaces
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- B01J2219/00677—Ex-situ synthesis followed by deposition on the substrate
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00718—Type of compounds synthesised
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00718—Type of compounds synthesised
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Abstract
The present invention relates to novel methods of the quantitative detection of molecules in an array. In particular, the present invention relates to methods for molecular detection assays performed on solid surfaces. The present invention provides improved methods for the high throughput analysis of molecular interactions and quantitative detection. In another aspect, the invention relates to a method of measuring protein interactions on a solid surface that is useful for the determination of equilibrium binding and rate constants. In yet another aspect, the invention relates to predicting a molecules utility in a detection assay.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US98350707P | 2007-10-29 | 2007-10-29 | |
US60/983,507 | 2007-10-29 |
Publications (2)
Publication Number | Publication Date |
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WO2009058867A2 WO2009058867A2 (en) | 2009-05-07 |
WO2009058867A3 true WO2009058867A3 (en) | 2009-07-23 |
Family
ID=40591736
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2008/081587 WO2009058867A2 (en) | 2007-10-29 | 2008-10-29 | Affinity measurements using frameless multiplexed microarrays |
Country Status (1)
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WO (1) | WO2009058867A2 (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9018584B2 (en) | 2011-04-14 | 2015-04-28 | Emd Millipore Corporation | Devices and methods for infrared (IR) based quantitation of biomolecules |
EP2831587B1 (en) | 2012-03-27 | 2018-05-16 | Ventana Medical Systems, Inc. | Signaling conjugates and methods of use |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6406921B1 (en) * | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
US20020127589A1 (en) * | 2001-03-08 | 2002-09-12 | Keiichi Sato | Microarray and microarray substrate |
KR20040047144A (en) * | 2002-11-29 | 2004-06-05 | 신-황 첸 | Microvolume detecting method and device |
KR20060122954A (en) * | 2004-03-01 | 2006-11-30 | 구라시키 보세키 가부시키가이샤 | Hybridization method as well as hybridization microarray and hybridization kit |
-
2008
- 2008-10-29 WO PCT/US2008/081587 patent/WO2009058867A2/en active Application Filing
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6406921B1 (en) * | 1998-07-14 | 2002-06-18 | Zyomyx, Incorporated | Protein arrays for high-throughput screening |
US20020127589A1 (en) * | 2001-03-08 | 2002-09-12 | Keiichi Sato | Microarray and microarray substrate |
KR20040047144A (en) * | 2002-11-29 | 2004-06-05 | 신-황 첸 | Microvolume detecting method and device |
KR20060122954A (en) * | 2004-03-01 | 2006-11-30 | 구라시키 보세키 가부시키가이샤 | Hybridization method as well as hybridization microarray and hybridization kit |
Also Published As
Publication number | Publication date |
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WO2009058867A2 (en) | 2009-05-07 |
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