WO2009045075A3 - A monoclonal antibody specific to human embryonic stem cell, a hybridoma secreting the same and a method for detecting or isolating non-differenced embryonic stem cell - Google Patents

A monoclonal antibody specific to human embryonic stem cell, a hybridoma secreting the same and a method for detecting or isolating non-differenced embryonic stem cell Download PDF

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Publication number
WO2009045075A3
WO2009045075A3 PCT/KR2008/005832 KR2008005832W WO2009045075A3 WO 2009045075 A3 WO2009045075 A3 WO 2009045075A3 KR 2008005832 W KR2008005832 W KR 2008005832W WO 2009045075 A3 WO2009045075 A3 WO 2009045075A3
Authority
WO
WIPO (PCT)
Prior art keywords
embryonic stem
stem cell
detecting
cells
differenced
Prior art date
Application number
PCT/KR2008/005832
Other languages
French (fr)
Other versions
WO2009045075A2 (en
Inventor
Hyo Jeong Hong
Yeon Sung Son
Original Assignee
Korea Res Inst Of Bioscience
Hyo Jeong Hong
Yeon Sung Son
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Res Inst Of Bioscience, Hyo Jeong Hong, Yeon Sung Son filed Critical Korea Res Inst Of Bioscience
Publication of WO2009045075A2 publication Critical patent/WO2009045075A2/en
Publication of WO2009045075A3 publication Critical patent/WO2009045075A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto

Abstract

Disclosed herein is the use of L1CAM, which is a marker protein of undifferentiated human embryonic stem (ES) cells. Also disclosed are an antibody binding specifically to L1CAM, a hybridoma secreting the antibody, and a method of detecting, identifying or isolating undifferentiated human ES cells using the antibody. Since antibodies binding specifically to L1CAM, including a novel antibody 4-63 according to the present invention, bind specifically to the L1CAM protein expressed on undifferentiated human ES cells, they are useful in the accurate analysis of characteristics of undifferentiated human ES cells and in isolating human ES cells for cell therapy.
PCT/KR2008/005832 2007-10-02 2008-10-02 A monoclonal antibody specific to human embryonic stem cell, a hybridoma secreting the same and a method for detecting or isolating non-differenced embryonic stem cell WO2009045075A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2007-0099513 2007-10-02
KR1020070099513A KR101302711B1 (en) 2007-10-02 2007-10-02 A monoclonal antibody specific to human embryonic stem sell, a hybridoma secreting the same and a method for detecting or isolating non-differenced embryonic stem cell

Publications (2)

Publication Number Publication Date
WO2009045075A2 WO2009045075A2 (en) 2009-04-09
WO2009045075A3 true WO2009045075A3 (en) 2009-05-22

Family

ID=40526844

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2008/005832 WO2009045075A2 (en) 2007-10-02 2008-10-02 A monoclonal antibody specific to human embryonic stem cell, a hybridoma secreting the same and a method for detecting or isolating non-differenced embryonic stem cell

Country Status (2)

Country Link
KR (1) KR101302711B1 (en)
WO (1) WO2009045075A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104936984A (en) * 2012-11-16 2015-09-23 江原大学校产学协力团 Antibody binding specifically to human and mouse L1cam protein, and use therefor

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101853418B1 (en) * 2010-06-16 2018-05-02 미네르바 바이오테크놀로지 코포레이션 Reprogramming Cancer Cells
KR101335485B1 (en) * 2011-06-09 2013-12-02 연세대학교 산학협력단 Methods for Separating and Culturing of Neural Precursor Cell from Pluripotent Stem Cells
KR101630225B1 (en) 2013-11-29 2016-06-14 세종대학교산학협력단 Monoclonal antibody 4A68 specific to PGRMC1 expressed on the surface of human embryonic stem cells and various cancer cells

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
APPEL, F. ET AL.: "Identification of the border between fibronectin type III homologous repeats 2 and 3 of the neural cell adhesion molecule Ll as a neurite outgrowth promoting and signal transducing domain.", JOURNAL OF NEUROBIOLOGY., vol. 28, no. 3, 1995, pages 297 - 312 *
BLACKMORE, M. ET AL.: "Ll, betal integrin, and cadherins mediate axonal regeneration in the embryonic spinal cord.", JOURNAL OF NEUROBIOLOGY., vol. 66, no. 14, December 2006 (2006-12-01), pages 1564 - 1583 *
FENDERSON, B. A. ET AL.: "Staining embryonic stem cells using monoclonal antibodies to stage specific embryonic antigens.", METHODS IN MOLECULAR BIOLOGY., vol. 325, 2006, pages 207 - 224 *
PRUSZAK, J. ET AL.: "Markers and methods for cell sorting of human embryonic stem cell-derived neural cell populations.", STEM CELLS., vol. 25, no. 9, 21 June 2007 (2007-06-21), pages 2257 - 2268 *
VAN HOOF, D. ET AL.: "A quest for human and mouse embryonic stem cell-specific proteins.", MOLECULAR AND CELLULAR PROTEOMICS., vol. 5, no. 7, 6 April 2006 (2006-04-06), pages 1261 - 1273 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104936984A (en) * 2012-11-16 2015-09-23 江原大学校产学协力团 Antibody binding specifically to human and mouse L1cam protein, and use therefor
CN104936984B (en) * 2012-11-16 2018-09-14 江原大学校产学协力团 Specifically bind the antibody and application thereof of people and mouse L1CAM

Also Published As

Publication number Publication date
KR20090034242A (en) 2009-04-07
KR101302711B1 (en) 2013-09-03
WO2009045075A2 (en) 2009-04-09

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