WO2007107304A3 - An efficient method for loading amphoteric liposomes with nucleic acid active substances - Google Patents

An efficient method for loading amphoteric liposomes with nucleic acid active substances Download PDF

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Publication number
WO2007107304A3
WO2007107304A3 PCT/EP2007/002349 EP2007002349W WO2007107304A3 WO 2007107304 A3 WO2007107304 A3 WO 2007107304A3 EP 2007002349 W EP2007002349 W EP 2007002349W WO 2007107304 A3 WO2007107304 A3 WO 2007107304A3
Authority
WO
WIPO (PCT)
Prior art keywords
liposomes
amphoteric liposomes
nucleic acid
loading
active substances
Prior art date
Application number
PCT/EP2007/002349
Other languages
French (fr)
Other versions
WO2007107304A2 (en
Inventor
Steffen Panzner
Gerold Endert
Una Rauchhaus
Natalie Herzog
Natalie Muller
Original Assignee
Novosom Ag
Steffen Panzner
Gerold Endert
Una Rauchhaus
Natalie Herzog
Natalie Muller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2006/009013 external-priority patent/WO2007031333A2/en
Priority claimed from EP06255277A external-priority patent/EP1911443A1/en
Priority claimed from US11/581,054 external-priority patent/US20080088046A1/en
Priority claimed from DE102006054192A external-priority patent/DE102006054192A1/en
Application filed by Novosom Ag, Steffen Panzner, Gerold Endert, Una Rauchhaus, Natalie Herzog, Natalie Muller filed Critical Novosom Ag
Priority to EP07723327A priority Critical patent/EP2004141A2/en
Priority to US12/225,030 priority patent/US20120021042A1/en
Publication of WO2007107304A2 publication Critical patent/WO2007107304A2/en
Publication of WO2007107304A3 publication Critical patent/WO2007107304A3/en
Priority to US14/066,616 priority patent/US20140056970A1/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1271—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers
    • A61K9/1272—Non-conventional liposomes, e.g. PEGylated liposomes, liposomes coated with polymers with substantial amounts of non-phosphatidyl, i.e. non-acylglycerophosphate, surfactants as bilayer-forming substances, e.g. cationic lipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A61K9/1277—Processes for preparing; Proliposomes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/582—Recycling of unreacted starting or intermediate materials

Abstract

A method for preparing amphoteric liposomes loaded with a polyanionic active agent as cargo, characterised by admixing an aqueous solution of said polyanionic active agent and an alcoholic solution of one or more amphiphiles and buffering said admixture to an acidic pH, said one or more amphiphiles being susceptible of forming amphoteric liposomes at said acidic pH, thereby to form such amphoteric liposomes in suspension encapsulating said active agent under conditions such that said liposomes form aggregates, and thereafter treating said suspension to dissociate said aggregates. Also disclosed are nucleic acid loaded amphoteric liposomes produced in accordance with the method, wherein said nucleic acids are oligonucleotides and said liposomes are multilamellar.
PCT/EP2007/002349 2005-09-15 2007-03-16 An efficient method for loading amphoteric liposomes with nucleic acid active substances WO2007107304A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP07723327A EP2004141A2 (en) 2006-03-17 2007-03-16 An efficient method for loading amphoteric liposomes with nucleic acid active substances
US12/225,030 US20120021042A1 (en) 2005-09-15 2007-03-16 Efficient Method For Loading Amphoteric Liposomes With Nucleic Acid Active Substances
US14/066,616 US20140056970A1 (en) 2005-09-15 2013-10-29 Efficient method for loading amphoteric liposomes with nucleic acid active substances

Applications Claiming Priority (16)

Application Number Priority Date Filing Date Title
DE102006013121 2006-03-17
DE102006013121.5 2006-03-17
EP06113784.0 2006-05-10
EP06113784 2006-05-10
EP06254821.9 2006-09-15
PCT/EP2006/009013 WO2007031333A2 (en) 2005-09-15 2006-09-15 Improvements in or relating to amphoteric liposomes
US11/521,857 2006-09-15
EPPCT/EP2006/009013 2006-09-15
US11/521,857 US20070104775A1 (en) 2005-09-15 2006-09-15 Amphoteric liposomes
EP06254821.9A EP1764091B1 (en) 2005-09-15 2006-09-15 Improvements in or relating to amphoteric liposomes
EP06255277A EP1911443A1 (en) 2006-10-13 2006-10-13 Amphoteric liposomes, method of formulating an amphoteric liposome and a method of loading an amphoteric liposome
US11/581,054 2006-10-13
US11/581,054 US20080088046A1 (en) 2006-10-13 2006-10-13 Amphoteric liposomes, a method of formulating an amphoteric liposome and a method of loading an amphoteric liposome
EP06255277.3 2006-10-13
DE102006054192.8 2006-11-10
DE102006054192A DE102006054192A1 (en) 2006-03-17 2006-11-10 Method for loading nucleic acid active substances within amphoteric liposomes, comprises: mixing lipid mixture solution and aqueous solution of nucleic acid active substances; and sterile filtrating the liposomes containing the substances

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US11/581,054 Continuation US20080088046A1 (en) 2005-09-15 2006-10-13 Amphoteric liposomes, a method of formulating an amphoteric liposome and a method of loading an amphoteric liposome

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US12/225,030 A-371-Of-International US20120021042A1 (en) 2005-09-15 2007-03-16 Efficient Method For Loading Amphoteric Liposomes With Nucleic Acid Active Substances
US14/066,616 Continuation US20140056970A1 (en) 2005-09-15 2013-10-29 Efficient method for loading amphoteric liposomes with nucleic acid active substances

Publications (2)

Publication Number Publication Date
WO2007107304A2 WO2007107304A2 (en) 2007-09-27
WO2007107304A3 true WO2007107304A3 (en) 2008-06-12

Family

ID=38235159

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2007/002349 WO2007107304A2 (en) 2005-09-15 2007-03-16 An efficient method for loading amphoteric liposomes with nucleic acid active substances

Country Status (2)

Country Link
EP (1) EP2004141A2 (en)
WO (1) WO2007107304A2 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4082551A1 (en) 2006-08-08 2022-11-02 Rheinische Friedrich-Wilhelms-Universität Bonn Structure and use of 5' phosphate oligonucleotides
EP2462924A3 (en) * 2006-10-13 2013-01-23 Marina Biotech, Inc. Improvements in or relating to amphoteric liposomes.
JP2010513354A (en) * 2006-12-19 2010-04-30 ノヴォソム アクチェンゲゼルシャフト Lipids and lipid aggregates containing transfection enhancer elements
EP2297323A1 (en) 2008-05-21 2011-03-23 Hartmann, Gunther 5' triphosphate oligonucleotide with blunt end and uses thereof
CA2739046A1 (en) * 2008-10-16 2010-04-22 Marina Biotech, Inc. Process and compositions for liposomal and effective delivery of nucleic acids
US20120195958A1 (en) * 2009-03-27 2012-08-02 National University Of Ireland, Galway Implantable devices
CN102481256B (en) 2009-07-09 2015-02-25 玛瑞纳生物技术有限公司 Amphoteric liposomes comprising imino lipids
ES2770335T3 (en) 2010-07-06 2020-07-01 Glaxosmithkline Biologicals Sa RNA administration to trigger multiple immune pathways
PL2590626T3 (en) 2010-07-06 2016-04-29 Glaxosmithkline Biologicals Sa Liposomes with lipids having an advantageous pka-value for rna delivery
WO2012006369A2 (en) 2010-07-06 2012-01-12 Novartis Ag Immunisation of large mammals with low doses of rna
LT3981427T (en) 2010-08-31 2022-08-10 Glaxosmithkline Biologicals S.A. Pegylated liposomes for delivery of immunogen-encoding rna
MX363307B (en) 2010-10-11 2019-03-20 Novartis Ag Star Antigen delivery platforms.
BR122022001262B1 (en) 2010-11-15 2022-09-27 Life Technologies Corporation TRANSFECTION COMPOUNDS CONTAINING AMINE AND TRANSFECTION COMPLEX
EP2508530A1 (en) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
CA2840989A1 (en) 2011-07-06 2013-01-10 Novartis Ag Immunogenic combination compositions and uses thereof
EP2729126B1 (en) * 2011-07-06 2020-12-23 GlaxoSmithKline Biologicals SA Liposomes having useful n:p ratio for delivery of rna molecules
EP2712870A1 (en) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
WO2016045732A1 (en) * 2014-09-25 2016-03-31 Biontech Rna Pharmaceuticals Gmbh Stable formulations of lipids and liposomes
CN104922068B (en) * 2015-05-22 2017-10-13 江苏凯基生物技术股份有限公司 A kind of Decoy nucleic acid cationics liposome vectors and preparation method thereof
US9931298B2 (en) * 2015-05-26 2018-04-03 Comfort Care For Animals Llc Liposome loading
AU2018307509B2 (en) 2017-07-24 2021-04-29 Pharmosa Biopharm Inc. Liposome compositions comprising weak acid drugs and uses thereof
WO2021255262A1 (en) 2020-06-19 2021-12-23 Sylentis Sau siRNA AND COMPOSITIONS FOR PROPHYLACTIC AND THERAPEUTIC TREATMENT OF VIRUS DISEASES
EP4015634A1 (en) 2020-12-15 2022-06-22 Sylentis, S.A.U. Sirna and compositions for prophylactic and therapeutic treatment of virus diseases
WO2023057596A1 (en) * 2021-10-06 2023-04-13 Leon-Nanodrugs Gmbh Method for preparing lipid nanoparticles

Citations (4)

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WO1998051278A2 (en) * 1997-05-14 1998-11-19 Inex Pharmaceuticals Corporation High efficiency encapsulation of charged therapeutic agents in lipid vesicles
WO2001005374A1 (en) * 1999-07-15 2001-01-25 Inex Pharmaceuticals Corp. Methods for preparation of lipid-encapsulated therapeutic agents
WO2006053646A2 (en) * 2004-11-19 2006-05-26 Novosom Ag Improvements in or relating to pharmaceutical compositions for local administration
WO2007012191A1 (en) * 2005-07-27 2007-02-01 Protiva Biotherapeutics, Inc. Systems and methods for manufacturing liposomes

Family Cites Families (4)

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DE102004054730A1 (en) * 2004-03-28 2006-05-11 Novosom Ag Serum stable amphoteric liposomes
AU2005300638A1 (en) * 2004-11-05 2006-05-11 Novosom Ag Improvements in or relating to pharmaceutical compositions comprising an oligonucleotide as an active agent
AU2006291429B2 (en) * 2005-09-15 2013-04-04 Biontech Delivery Technologies Gmbh Improvements in or relating to amphoteric liposomes
WO2007064857A2 (en) * 2005-12-01 2007-06-07 Pronai Therapeutics, Inc. Amphoteric liposome formulation

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998051278A2 (en) * 1997-05-14 1998-11-19 Inex Pharmaceuticals Corporation High efficiency encapsulation of charged therapeutic agents in lipid vesicles
WO2001005374A1 (en) * 1999-07-15 2001-01-25 Inex Pharmaceuticals Corp. Methods for preparation of lipid-encapsulated therapeutic agents
WO2006053646A2 (en) * 2004-11-19 2006-05-26 Novosom Ag Improvements in or relating to pharmaceutical compositions for local administration
WO2007012191A1 (en) * 2005-07-27 2007-02-01 Protiva Biotherapeutics, Inc. Systems and methods for manufacturing liposomes

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP2004141A2 *

Also Published As

Publication number Publication date
WO2007107304A2 (en) 2007-09-27
EP2004141A2 (en) 2008-12-24

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