WO2007024264A3 - Method and system for digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation - Google Patents

Method and system for digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation Download PDF

Info

Publication number
WO2007024264A3
WO2007024264A3 PCT/US2006/006530 US2006006530W WO2007024264A3 WO 2007024264 A3 WO2007024264 A3 WO 2007024264A3 US 2006006530 W US2006006530 W US 2006006530W WO 2007024264 A3 WO2007024264 A3 WO 2007024264A3
Authority
WO
WIPO (PCT)
Prior art keywords
cytoplasm
pixels
quantitation
nucleus
membrane
Prior art date
Application number
PCT/US2006/006530
Other languages
French (fr)
Other versions
WO2007024264A2 (en
Inventor
Abhijeet S Gholap
Gauri A Gholap
Aparna Joshi
Amitabha Basu
C V K Rao
Prithviraj Jadhav
Original Assignee
Bioimagene Inc
Abhijeet S Gholap
Gauri A Gholap
Aparna Joshi
Amitabha Basu
C V K Rao
Prithviraj Jadhav
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioimagene Inc, Abhijeet S Gholap, Gauri A Gholap, Aparna Joshi, Amitabha Basu, C V K Rao, Prithviraj Jadhav filed Critical Bioimagene Inc
Publication of WO2007024264A2 publication Critical patent/WO2007024264A2/en
Publication of WO2007024264A3 publication Critical patent/WO2007024264A3/en

Links

Classifications

    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/10—Segmentation; Edge detection
    • G06T7/11—Region-based segmentation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/695—Preprocessing, e.g. image segmentation
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING; CALCULATING OR COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro

Landscapes

  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Molecular Biology (AREA)
  • Multimedia (AREA)
  • Medical Informatics (AREA)
  • Biomedical Technology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

A method and system for automatic digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation. Plural types of pixels comprising cell components including at least cell cytoplasm and cell membranes from a biological tissue sample to which a chemical compound has been applied and has been processed to remove background pixels and pixels including counterstained components are simultaneously identified. The identified cell components pixels are automatically classified to determine a medical conclusion such as a human breast cancer, a human prostrate cancer or an animal cancer.
PCT/US2006/006530 2005-02-23 2006-02-23 Method and system for digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation WO2007024264A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US65546505P 2005-02-23 2005-02-23
US60/655,465 2005-02-23

Publications (2)

Publication Number Publication Date
WO2007024264A2 WO2007024264A2 (en) 2007-03-01
WO2007024264A3 true WO2007024264A3 (en) 2007-04-19

Family

ID=36676002

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2006/006530 WO2007024264A2 (en) 2005-02-23 2006-02-23 Method and system for digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation

Country Status (1)

Country Link
WO (1) WO2007024264A2 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9240043B2 (en) 2008-09-16 2016-01-19 Novartis Ag Reproducible quantification of biomarker expression

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
EP2029779A4 (en) 2006-06-14 2010-01-20 Living Microsystems Inc Use of highly parallel snp genotyping for fetal diagnosis
ATE528722T1 (en) 2007-05-14 2011-10-15 Historx Inc DEPARTMENT SEPARATION THROUGH PIXEL CHARACTERIZATION USING IMAGE DATA CLUSTERING
CA2604317C (en) 2007-08-06 2017-02-28 Historx, Inc. Methods and system for validating sample images for quantitative immunoassays
EP3245069B1 (en) 2015-04-20 2018-09-12 Koninklijke Philips N.V. Printing control device and method to control printing of a cover layer on a sample
EP3308327A4 (en) * 2015-06-11 2019-01-23 University of Pittsburgh - Of the Commonwealth System of Higher Education Systems and methods for finding regions of interest in hematoxylin and eosin (h&e) stained tissue images and quantifying intratumor cellular spatial heterogeneity in multiplexed/hyperplexed fluorescence tissue images
US9779499B2 (en) * 2015-09-18 2017-10-03 Definiens Ag Grading of glandular tissue cancer by detailed image analysis of stained tissue slices

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003030088A1 (en) * 2001-10-04 2003-04-10 Chromavision Medical Systems, Inc. Method and apparatus for scoring the uptake of markers in cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003030088A1 (en) * 2001-10-04 2003-04-10 Chromavision Medical Systems, Inc. Method and apparatus for scoring the uptake of markers in cells

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
TABESH A ET AL: "Automated prostate cancer diagnosis and Gleason grading of tissue microarrays", PROCEEDINGS OF THE SPIE, SPIE, BELLINGHAM, VA, US, vol. 5747, no. 1, 17 February 2005 (2005-02-17), pages 58 - 70, XP002408570, ISSN: 0277-786X *
TEVEROVSKIY M ET AL: "Improved prediction of prostate cancer recurrence based on an automated tissue image analysis system", BIOMEDICAL IMAGING: MACRO TO NANO, 2004. IEEE INTERNATIONAL SYMPOSIUM ON ARLINGTON,VA, USA APRIL 15-18, 2004, PISCATAWAY, NJ, USA,IEEE, 15 April 2004 (2004-04-15), pages 257 - 260, XP010773846, ISBN: 0-7803-8389-3 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US9273355B2 (en) 2006-06-14 2016-03-01 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8372584B2 (en) 2006-06-14 2013-02-12 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8168389B2 (en) 2006-06-14 2012-05-01 The General Hospital Corporation Fetal cell analysis using sample splitting
US9017942B2 (en) 2006-06-14 2015-04-28 The General Hospital Corporation Rare cell analysis using sample splitting and DNA tags
US8137912B2 (en) 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
US9347100B2 (en) 2006-06-14 2016-05-24 Gpb Scientific, Llc Rare cell analysis using sample splitting and DNA tags
US9240043B2 (en) 2008-09-16 2016-01-19 Novartis Ag Reproducible quantification of biomarker expression
US8195415B2 (en) 2008-09-20 2012-06-05 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US8296076B2 (en) 2008-09-20 2012-10-23 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuoploidy by sequencing
US8682594B2 (en) 2008-09-20 2014-03-25 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9353414B2 (en) 2008-09-20 2016-05-31 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing
US9404157B2 (en) 2008-09-20 2016-08-02 The Board Of Trustees Of The Leland Stanford Junior University Noninvasive diagnosis of fetal aneuploidy by sequencing

Also Published As

Publication number Publication date
WO2007024264A2 (en) 2007-03-01

Similar Documents

Publication Publication Date Title
WO2007024264A3 (en) Method and system for digital image based tissue independent simultaneous nucleus, cytoplasm and membrane quantitation
EP2995195A3 (en) Method and system for monitoring the condition of livestock
WO2007099525A3 (en) System and method of automatic prioritization and analysis of medical images
WO2007109771A3 (en) Automated lesion analysis based upon automatic plaque characterization according to a classification criterion
GB2423151B (en) Examination systems for biological samples
EP4101537A3 (en) Intergrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
WO2007109639A3 (en) Cytoblock preparation system and methods of use
WO2006127860A3 (en) Multiplex method for increased proteomic coverage from histopathologically processed biological samples using liquid tissue preparations
EP4105644A3 (en) Systems and devices for sequence by synthesis analysis
EP1887357A3 (en) Analyzer and analyzing method
DE602004017044D1 (en) MILBENPRODUKT, ITS USE, METHOD OF RESTORING THE PHYTOSEIID RARE MILBER AMBLYSEIUS SIRSKII, RESTORATION SYSTEM FOR THE FARMING OF THIS PHYTOSEIID MILK, AND BIOLOGICAL PESTICULAR FIGHTING PROCEDURES ON A CULTURE
EP1832894A3 (en) System and method for automatic gain compensation based on image processing
WO2008019311A3 (en) Automated cassette and slide handling system for an automatic microscope
WO2007080580A3 (en) System and method for segmenting structures in a series of images
WO2006085906A3 (en) Method for the direct detection of diagnostic rna
WO2008008515A3 (en) Methods and compositions for detecting rare cells from a biological sample
EP2260942A3 (en) Protease Inhibitor Sample Collection System
WO2012103025A3 (en) Methods for obtaining single cells and applications of single cell omics
WO2005033010A3 (en) Waste water treatment system and process
EP1710562A3 (en) Microfluidic system and method of utilization
EP1783204A3 (en) Biological sample handling and imaging
GB0710122D0 (en) Mite composition use thereof method for rearing a mite rearing system use of the composition in biological pest control and method of biological pest control
EP2073475A3 (en) System for processing common gain values
TW200741560A (en) Systems and methods for emphasized cytological specimen review
EP2355555A3 (en) Musical tone signal-processing apparatus

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application
NENP Non-entry into the national phase

Ref country code: DE

122 Ep: pct application non-entry in european phase

Ref document number: 06735978

Country of ref document: EP

Kind code of ref document: A2