WO2006025863A3 - Simultaneous detection of metabolic enzyme activity and metabolite levels - Google Patents

Simultaneous detection of metabolic enzyme activity and metabolite levels Download PDF

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Publication number
WO2006025863A3
WO2006025863A3 PCT/US2005/006371 US2005006371W WO2006025863A3 WO 2006025863 A3 WO2006025863 A3 WO 2006025863A3 US 2005006371 W US2005006371 W US 2005006371W WO 2006025863 A3 WO2006025863 A3 WO 2006025863A3
Authority
WO
WIPO (PCT)
Prior art keywords
metabolic
product
enzymes
enzyme activity
analytes
Prior art date
Application number
PCT/US2005/006371
Other languages
French (fr)
Other versions
WO2006025863A2 (en
Inventor
Blas Cerda
Original Assignee
Perkinelmer Las Inc
Blas Cerda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US2004/028238 external-priority patent/WO2005021779A1/en
Application filed by Perkinelmer Las Inc, Blas Cerda filed Critical Perkinelmer Las Inc
Priority to US10/539,180 priority Critical patent/US7485435B2/en
Publication of WO2006025863A2 publication Critical patent/WO2006025863A2/en
Publication of WO2006025863A3 publication Critical patent/WO2006025863A3/en
Priority to US12/349,669 priority patent/US8318417B2/en
Priority to US13/357,324 priority patent/US8399186B2/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/04—Endocrine or metabolic disorders

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Provided are methods for detecting a metabolic disorder in an individual using mass spectrometry. One method involves (a) contacting a sample containing (i) one or more metabolically indicative enzymes and (ii) one or more metabolic analytes, with one or more substrates for said one or more enzymes to produce a reaction admixture, under conditions wherein at least one of said enzymes is capable of acting on a corresponding substrate to generate at least one product, and, wherein one or more protease inhibitors are present; (b) contacting said reaction admixture with a reagent that inhibits the ability of said one or more enzymes to act on a corresponding substrate, wherein said one or more metabolic analytes and said at least one product are soluble in said reagent; to produce a test sample and (c) determining the presence or amount of said one or more metabolic analytes and said at least one product contained in said test sample using mass spectrometry, wherein a determined presence or amount of said one or more metabolic analytes and said at least one product correlates with presence or absence of said metabolic disorder. product correlates with presence or absence of said metabolic disorder.
PCT/US2005/006371 2003-08-29 2005-02-28 Simultaneous detection of metabolic enzyme activity and metabolite levels WO2006025863A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US10/539,180 US7485435B2 (en) 2004-08-30 2005-02-28 Simultaneous detection of metabolic enzyme activity and metabolite levels
US12/349,669 US8318417B2 (en) 2003-08-29 2009-01-07 Simultaneous detection of metabolic enzyme activity and metabolite levels
US13/357,324 US8399186B2 (en) 2003-08-29 2012-01-24 Simultaneous detection of metabolic enzyme activity and metabolite levels

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
USPCT/US04/28238 2004-08-30
PCT/US2004/028238 WO2005021779A1 (en) 2003-08-29 2004-08-30 Mass spectrometry methods for simultaneous detection of metabolic enzyme activity and metabolite levels

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US10/652,732 Continuation US20050048499A1 (en) 2003-08-29 2003-08-29 Tandem mass spectrometry method for the genetic screening of inborn errors of metabolism in newborns

Related Child Applications (2)

Application Number Title Priority Date Filing Date
US10/539,180 A-371-Of-International US7485435B2 (en) 2003-08-29 2005-02-28 Simultaneous detection of metabolic enzyme activity and metabolite levels
US12/349,669 Continuation US8318417B2 (en) 2003-08-29 2009-01-07 Simultaneous detection of metabolic enzyme activity and metabolite levels

Publications (2)

Publication Number Publication Date
WO2006025863A2 WO2006025863A2 (en) 2006-03-09
WO2006025863A3 true WO2006025863A3 (en) 2007-11-22

Family

ID=36000463

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2005/006371 WO2006025863A2 (en) 2003-08-29 2005-02-28 Simultaneous detection of metabolic enzyme activity and metabolite levels

Country Status (1)

Country Link
WO (1) WO2006025863A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8628936B2 (en) 2007-12-05 2014-01-14 King's College London Methods and compositions for multiplexed screening of disease

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096812A (en) * 1989-06-08 1992-03-17 Osborn Laboratories, Inc. Assay method for gamma glutamyltransferase (GGT) in liquid blood and dried blood
US6258605B1 (en) * 1999-03-26 2001-07-10 Neo Gen Screening, Inc. Clinical method for the genetic screening of newborns using tandem mass spectrometry
US6455321B1 (en) * 1999-01-30 2002-09-24 Neo Gen Screening, Inc. Method for interpreting tandem mass spectrometry data for clinical diagnosis

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096812A (en) * 1989-06-08 1992-03-17 Osborn Laboratories, Inc. Assay method for gamma glutamyltransferase (GGT) in liquid blood and dried blood
US6455321B1 (en) * 1999-01-30 2002-09-24 Neo Gen Screening, Inc. Method for interpreting tandem mass spectrometry data for clinical diagnosis
US6258605B1 (en) * 1999-03-26 2001-07-10 Neo Gen Screening, Inc. Clinical method for the genetic screening of newborns using tandem mass spectrometry

Also Published As

Publication number Publication date
WO2006025863A2 (en) 2006-03-09

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