WO2005070438A1

WO2005070438A1 - Pharmaceutical composition comprising water depleted of heavy isotopes 0-17 and 0-18

Info

Publication number: WO2005070438A1
Application number: PCT/RU2004/000019
Authority: WO
Inventors: Igor Anatolievich Pomytkin; Sergey Pavlovich Soloviev
Original assignee: Igor Anatolievich Pomytkin; Sergey Pavlovich Soloviev
Priority date: 2004-01-23
Filing date: 2004-01-23
Publication date: 2005-08-04

Abstract

The invention relates to the compositions and methods comprising water depleted of heavy isotope for treating cancer in mammal in need thereof, wherein the heavy isotope is at least one isotope selected from the group concisting of ¹⁷O and ¹⁸O. Preferably, ¹⁷O content in the water is from 1 to 385 ppm, and ¹⁸O content in the water is from 10 to 1970 ppm. Preferably, the mammal is human.

Description

PHARMACEUTICAL COMPOSITION COMPRISING WATER DEPLETED OF HEAVY ISOTOPES 0-17 AND 0-18

5 Technical Field

The present invention is in the field of healthcare. More specifically, this invention relates to compositions and methods for treating cancer comprising water depleted of heavy isotopes, wherein the heavy isotopes are ^I7O and ¹⁸O. 10 Background Art

As defined, cancer is a group of diseases that are each characterized by the uncontrolled growth of a particular type of cell. It begins in a tissue containing such a cell and, if the cancer has not spread to any additional tissues at the time of diagnosis, 15 may be treated by, for example, surgery, radiation, or another type of localized therapy. US Patent No. 5,855,921 discloses products for curing tumorous diseases, comprising as active agent water or aqueous solutons, suitable for human consumption, having a deuterium content of 0.1 to 110 ppm. 20 US Patent No. 5,788,953 discloses preparations are capable of preventing tumor formation, wherein the preparations contain, as essential component, water having a deuterium content of 11 1 to 135 ppm. RU Patent No. 2,125,817 discloses food products for preventing development of tumors comprising water having a deuterium content of 111 to 135 ppm. 25 Natural water is formed predominantly by stable isotopes of H hydrogen and O oxygen. The content of major water isotopic isomer Η₂ ¹⁶O in nature is about 99.76%. About 0.24% of natural water is composed by water isotopic isomers comprising at least one heavy isotope such as ²H, ¹⁷O, and ¹⁸O. Heavy isotopes content in natural water is not a constant value and depends on Earth district and climatic conditions. So, 30 Vienna's standard mid-ocean water (V-SMOW) determines content of heavy isotopes in water as 155.8 ppm for of ²H and 2005 ppm for ¹⁸O isotope. Yellowstone park snow (NBS-1A standard) content of heavy isotopes in water is 127.2 ppm for of ²H and 1956 ppm for ¹⁸O isotope. A content of heavy isotopes in water from Potomac River (NBS-1 standard) is 148.3 ppm for of ²H and 1989 ppm for ¹⁸O isotope. Greenland ice precipitation (GISP standard) content of heavy isotopes in water is 126.2 ppm for of ²H and 1957 ppm for ¹⁸O isotope. Standard light Antarctic precipitation (SLAP) content of heavy isotopes in water is 89.1 ppm for of ²H and 1894 ppm for ¹⁸O isotope. Ferronsky V.F. et al., "Isotopy of the hydrosphere", Moscow, Nauka, 1983, p.10. So, on the territory of usual human residing, the ²H isotope content in natural water is from about 126.2 to 155.8 ppm and the ^lsO isotope content is from about 1956 to 2005 ppm. Abnormally low content of ²H isotope (< 127 ppm) and ¹⁸O isotope (<1957) was found out of area of usual human residing in water from Greenland ice and ancient Antarctic ice. Water with lower contents of heavy isotopes than natural is known in the art and can be prepared by industrial methods well-documented in the art. Unexpectedly, we discovered that natural water depleted of heavy isotopes ¹⁷O, and ¹⁸O is useful for treating cancer. It is an object of the present invention to provide compositions for treating cancer comprising water depleted of a heavy isotope, wherein the heavy isotope is at least one isotope selected from the group consisting of ¹⁷O and ¹⁸O. It is an object of the present invention to provide methods of treating cancer which comprises the step of administering to a mammal in need thereof water depleted of a heavy isotope, wherein the heavy isotope is at least one isotope selected from the group consisting of O, and O. Disclosure of Invention The present invention provides a composition for treating cancer comprising water depleted of a heavy isotope, wherein the heavy isotope is at least one isotope selected from the group consisting of ¹⁷O and ¹⁸O. Preferably, the water in the composition of the invention is water depleted of heavy isotopes ¹⁷O and ¹⁸O. Preferably, the composition comprises from about 0.1% to about 99.99%, by weight of 17 1 R the composition, of water depleted of heavy isotopes O and O. As used herein, the term "depleted" means that heavy isotope content in water is lower than minimal content observed in natural water on the territory of said mammal residing. Preferably, the ^I7O content in the water depleted of heavy isotope is from 1 to 385 ppm. Preferably, the ¹⁸O content in the water depleted of heavy isotope is from 10 to 1970 ppm. The compositions of the present invention can further comprise a number of optional components. Examples of suitable optional components include, but are not limited to, carbon dioxide; inorganic salts typically abandoned in natural drinking water such as sodium chloride, sodium bicarbonate, calcium chloride, magnesium sulfate; preserving agents; coloring agents; sweetening agents; and flavoring agents. The compositions of the present invention can be formulated in a wide variety of product forms, including but not limited to solutions, creams, ointments, aerosols, spray. Preferably, the composition for treating cancer is formulated as drinking water and does not have any added sweeteners or additives other than flavors, extracts or essences and any flavors or extracts that are added to the water are less than 1% of the final product. Also, the composition for treating cancer might be formulated as a beverage when any flavors or extracts that are added to the water depleted of a heavy isotope is more than 1% of the final product. Further, the present invention provides method of treating cancer which comprises the step of administering to a mammal in need thereof water depleted of a heavy isotope, wherein the heavy isotope is at least one isotope selected from the group consisting of ¹⁷O and ¹⁸O. Preferably, the water in the method of the invention is water depleted of heavy isotopes ¹⁷O and ¹⁸O. Preferably, the mammal is a human. Vater in the method of the invention is administered orally in amounts and daily regimen as recommended for natural water by dietarians or hygienists. Preferably, water of the invention is administered for 1 day or more. Preferably, water will used in amounts from about 1 to 3000 ml per day per mammalian subject. Preferably, the ¹⁷O content in the water depleted of heavy isotope is from 1 to 385 ppm. Preferably, the ¹⁸0 content in the water depleted of heavy isotope is from 10 to 1970 ppm. Water depleted of the heavy isotopes will usually prepared by industrial procedures well-known from the art or extracted from natural sources with low content of heavy isotopes such as Antarctic ice. The following examples are presented to demonstrate the invention. The examples are illustrative only and are not intended to limit the scope of the invention in any way.

Example 1 This example shows that heavy oxygen isotopes depleted water is useful for treating cancer. Materials. Heavy isotopes depleted water (HID water, 142 ppm ²H, 340 ppm ¹⁷O, and 1300 ppm ¹⁸O) was used. Natural water (142 ppm ²H, 395 ppm ¹⁷O, and 1983 ppm ¹⁸O) was used as control water. Treatment. Friend erythroleukemia-bearing C57BL male mice of 10- to 12- weeks aged and about 25g of weight were prepared by i.p. injection with 2*10° erythro leukemia tumor cells. Then, mice were randomized and drink HID water or control water ad libitum for 15 days. The effect of the treatments was determined by tumor growth delay in comparison with control to 16th day following tumor implantation. Data are presented in Table 1 as a tumor mass means ± SD (n=5 in each group). Statements referring to a significant difference indicate a p value of 0.05 or less. Table 1. Tumor growth.

^Differs significantly from control Table 1 shows that water depleted of heavy isotopes ¹⁷O and ¹⁸O is useful for treating cancer. Example 2 This example demonstrates composition comprising water depleted of heavy oxygen isotopes for treating cancer.

Claims

WE CLAIM:

1. A composition for treating cancer in a mammal in need thereof comprising water depleted of a heavy isotope, wherein the heavy isotope is at least one isotope selected from the group consisting of ¹⁷O and ¹⁸O.

2. The composition according to claim 1, wherein the water is water depleted of heavy isotopes ¹⁷O and ¹⁸O.

3. The composition according to claim 1, wherein the ¹⁷O content in the water is from 1 to 385 ppm.

4. The composition according to claim 1, wherein the O content in the water is from 10 to 1970 ppm.

5. The composition according to claim 1, wherein the composition comprises from about 0.1% to about 99.99%, by weight of the composition, of water depleted of a heavy isotope.

6. A method of treating cancer which comprises the step of administering to a mammal in need thereof water depleted of a heavy isotope, wherein the heavy isotope is at least one isotope selected from the group consisting of ¹⁷O and ¹⁸O.

7. The method according to claim 6, wherein the water is water depleted of heavy isotopes ¹⁷O and ¹⁸O.

8. The method according to claim 7, wherein the mammal is human.