WO2004109266A1

WO2004109266A1 - Dna detector

Info

Publication number: WO2004109266A1
Application number: PCT/KR2003/001140
Authority: WO
Inventors: Jun Keun Chang; Dong Chul Han
Original assignee: Digital Bio Technology
Priority date: 2003-06-10
Filing date: 2003-06-10
Publication date: 2004-12-16
Also published as: AU2003237050A1

Abstract

The DNA detector comprises a light source part (12). While the light from the light source part (12) passes the incident light filtering part (13), the light not in the desired wavelength range is removed. The passed light is incident in the sloped direction on at least one of DNA chip which is dotted on the slide (14) and wherein a fluorescent probe is applied. The position of the slide (14) is controlled by a transport device. While the light emitted from the fluorescent probe passes reflection light filtering part (16), the light not in the emission wavelength of the fluorescent probe is removed. The passed light is processed and imaged by a CCD camera (17). This image is interpreted by an image reading part (18). The CCD camera (17) photographs several times an area (15) of the samples, and the image reading part (18) interprets the several images by overlapping.

Description

DNA DETECTOR

Technical Field

The present invention relates to a DNA (deoxyribonucleic acid) detector. More particularly, the present invention relates to a DNA detector for reading and analyzing a DNA chip,, as a human tissue sample, in order to diagnose human diseases.

Background Art

Recently, a disease diagnostic technique using an "in-vitro diagnosis system" is rapidly spreading in the medical field. The "in-vitro diagnosis system" refers to diagnosing various human diseases, for example, genetic disorders through reading and analyzing a tissue sample extracted from a human body, that is, a DNA chip. To read and analyze this DNA chip, use is made of a DNA detector having a scanner for performing a quantitative analysis of light, which is cast onto and then reflected from the DNA chip.

Meanwhile, in case of analyzing such a DNA chip using the scanner, a fluorescent probe is used as an analytic medium. The fluorescent probe is a substance that, when a specific wavelength of light is cast on the substance, emits light having a specific wavelength longer than that of the cast light. In view of this property, when fluorescent probes having different optical properties from each other are used, some substances can be simultaneously examined. To cast a specific wavelength of light into such a fluorescent probe, there is needed an appropriate light source and an optical filter for selecting a desired wavelength of light alone. Further, to sense light emitted from the fluorescent probe, another optical filter must be used, by which the whole light emitted from fluorescent probe are collected, and then only the light corresponding to an emitting wavelength of the fluorescent probe is picked out. In addition, a sensor having an excellent photosensitivity must be used to measure a quantity of light.

In the scanner having this construction, a high density of polychromatic fluorescent probe is used to analyze many genetic diseases from a DNA chip to be detected at a time. Thus, to increase sensitivity and resolution, a laser is mainly used as a light source, and a photo multiplier tube (PMT) is used as a sensor. In particular, in case of using a mode of a point light source, the laser is possible to have its resolution of up to 5 ~ lO m, and the sensor as PMT is also possible to have its sensitivity of up to one molecule/ m .

To scan the whole area of a general slide having a width of 25 mm and a length of 75mm within 5 minutes, the scanner applies a device having a capability to transport the light source or the slide within a short time. In the mode of transporting the light source, there is used a mode in which a laser beam moves on the surface of the slide in a zigzag form while a mirror located on a path through which the laser beam passes is controlled at a fast speed. By contrast, in the mode of transporting the slide, a dedicated x-y stage is used on the whole.

Meanwhile, most of the scanners are used with a "confocal" mode so as to eliminate background noise components as many as possible from among fluorescent light emitted from the DNA chip, which is dotted on the surface of the slide. The "confocal" mode means a method of positioning a film, in which very small pin holes are perforated, on the path of light in order to allow only light cast at a specific distance to pass through.

A schematic construction of the general DNA detector using this confocal mode is shown in FIGs. 1 and 2. First, referring to FIG. 1, a laser beam emitted from a laser source (not shown) passes through a band-pass filter 1 allowing for a specific wavelength alone and then has its path turned at 90 degrees by means of a beam splitter 2. Subsequently, the laser beam is cast onto a slide 3 on which a DNA chip is dotted. In this case, the slide 3 is out of a focus of the laser beam as shown. Rays emitted by a fluorescent probe of the DNA chip are collected by an objective lens 4, and then are subjected to path change of 90 degrees again by another mirror 5. Then, the rays pass through another band-pass filter 6 allowing for only the light corresponding to an emitting wavelength of the fluorescent probe and then, are incidented onto a confocal film 8 through a sensing lens 7. Here, the rays fail to pass through pin holes 8a of the confocal film 8, because they are out of a focus as mentioned above.

By contrast, as shown in FIG. 2, if a laser beam is incidented and exactly focused onto a slide 3, the rays collected through a sensing lens 6 exactly pass through pin holes 8a of a confocal film 8, so that they can be incidented into a sensing part 9. Here, zigzag movement of a light source or the slide 3 is precisely controlled so as to scan the whole DNA chips dotted on the slide 3.

Signals sensed at the sensing part 9 are converted and stored into images by built-in processing parts. In this case, these images are represented into a very small dot at every moment. Therefore, an independent image processing software is required in order to collect, process and read all these images after all the examination area are scanned by the rays.

However, the conventional DNA detector has a disadvantage in that it is very expensive, because the PMT used as the sensor 9 is very expensive, and because it costs a great deal to implement a movable mechanism(device) for scanning the whole area of the slide 3 within about 5 minutes by means of several point light sources. To be more specific, the conventional DNA detector requires such expensive components, because it is designed to diagnose almost all diseases including a particular disease. However, it is frequently pointed out that there is no need to use such a conventional expensive DNA detector for a recent proposed DNA chip for diagnosing a particular disease.

Further, the conventional detector has another disadvantage in that the whole structure is very complicated due to a high-speed scanning mechanism.

Furthermore, because the conventional detector has an optical apparatus requiring a high precision, it has yet another disadvantage in that it is difficult to precisely control minute motion. In particular, a technician is needed for a precise adjustment of processed images after a motion is made.

To overcome the disadvantages of the DNA detector having the scanner of such a laser scanning mode, there is proposed another detector (US Patent No. 6,140,653), which is shown in FIG. 3. As for the detector shown in FIG. 3, a lamp 200 is used as a light source instead of a laser, and a CCD camera 180 which is less expensive than the PMT is used as a sensor.

The lamp 200 surrounds a reflector 220, in which the lamp 200 is arranged in a horizontal direction, in particular, to cast light in a direction parallel to a surface of a stage 140 on which a slide 120 is rested. At the front of the lamp 200, a heat sink mirror 240 for removing a hot heat of light and a filter 260 for transmitting only a selected frequency band are sequentially arranged. Light passing tlirough the filter 260 is adapted to be reflected upward by a slanted flat mirror 340, and then to be slantingly incidented onto the slide 120 by a concave mirror 320.

Light emitted from a fluorescent probe on the slide 120 passes through a pair of lenses 380 and 400 and a filter 420 interposed between the pair of lenses, and then incidented onto a CCD camera 180, so that a fluorescent image is generated from the CCD camera 180. This fluorescent image is read at an image processing part 500, so that it can be checked whether or not a disease is present.

h terms of price, the conventional detector shown in FIG. 3 has an advantage in that the scanner has a lowered price to a certain degree because the CCD camera is used as the sensor instead of the PMT. Nevertheless, the concave mirror is used as a means for slantingly incidenting the horizontal light, which is emitted from the lamp, on the slide. This concave mirror is very expensive. Consequently, the conventional detector shown in FIG. 3 does not seem to have a great advantage as compared with one having the scanner of the laser scanning mode. As known, the reason why the concave mirror is expensive results from costs needed to fabricate its curvature with precision according to an incident angle.

Disclosure of the Invention

Therefore, the present invention has been made in view of the abovementioned problems, and it is an object of the present invention to provide a DNA detector designed to use a lamp or a laser as a light source, and to use an inexpensive

CCD camera as a sensing means, and in particular to lessen the number of components, thereby allowing for being exclusively used for a recent available DNA chip for diagnosing particular diseases alone without a heavy burden on costs.

It is another object of the present invention to provide a DNA detector adapted to enable even a common user except for an expert to look at an output image of a CCD camera so as to interpret a disease without a dedicated image processing software.

In order to achieve the objects of the present invention, according to an aspect of the present invention, there is provided a DNA detector having a light source part operated by an electric power source part. While the light emitted from the light source part passes the incident light adjusting part, the light not in the desired wavelength band is removed. The light passing through the incident light adjusting part is slantingly incidented onto at least one of DNA chip which is dotted on the slide and covered with a fluorescent probe. The position of the slide is controlled by a transport device manipulated manually or automatically.

While the light emitted by the fluorescent probe passes through reflection light adjusting part, the light not in the emission wavelength of the fluorescent probe is removed. The light passing through the reflection light adjusting part is processed into an image by a CCD camera. This image is read by an image reading part, and thereby a user diagnoses a disease. The CCD camera photographs an examination area of the samples several times, and the image reading part overlaps and reads the photographed several images.

Meanwhile, a lamp or a laser beam may be used as a light source. First, when the lamp is used, the light source part includes the lamp. The incident light adjusting part includes a light adjusting lens unit for adjusting a quantity and a focal distance of the light, a light filter for selectively passing only a certain wavelength of light, and a light reflector for slantingly directing a path of the light onto a slide. The reflection light adjusting part includes a light filter for passing only light corresponding to an emission wavelength of the fluorescent probe, and a light adjusting lens unit for condensing light passing through the light filter.

When a halogen lamp is used as the lamp, the halogen lamp is enclosed by a condenser. The halogen lamp is slantingly arranged on a surface of the slide at a predetennined acute angle. A heat sinker, the light adjusting lens unit, and the light filter are sequentially arranged in front of the halogen lamp. The heat sinker is employed because it discharges much heat.

When a xenon lamp or a mercury lamp is used as the lamp, a constant voltage retained type of electric power source part is used to extend the lifetime of the lamp.

The xenon lamp or the mercury lamp is provided with a hemi-elliptical light reflector as the light condenser in order to condense the light. The xenon lamp or the mercury lamp is arranged in the same direction as the halogen lamp. The light filter and the light adjusting lens unit are sequentially arranged in front of the xenon lamp or the mercury lamp.

When a light emitting diode (LED) is used as the lamp, the light emitting diode, also, is slantingly arranged on a surface of the slide at a predetermined acute angle. The light filter and the light adjusting lens unit are slantingly arranged in that order in front of the light emitting diode at the same slant angle as that of the light emitting diode.

When the light source is a laser beam, the light source part includes a laser beam scanning section and a light modifier for changing a laser beam in a proper form. Only when the laser beam is subjected to total internal reflection in the slide, the light modifier is omitted because the laser beam must be generated in a dot form. The incident light adjusting part includes a light adjusting lens unit for controlling a path of the laser beam, a light reflector for changing the path of the laser beam, and a rotational driver for rotating the light reflector or the light adjusting lens unit. Meanwhile, the reflection light adjusting part and the light detecting part have the same construction as the previous one.

The light modifier comprises a collimating lens for focusing the laser beam, and a cylindrical lens for changing the laser beam into a linear form. When a total internal reflection mode without the light modifier is employed, the light adjusting lens unit comprises a prism for changing a path of the laser beam, and a cylindrical lens for adjusting a focus of the laser beam.

With the foregoing construction, while the light emitted from the light source passes through the incident light adjusting part, only a selected wavelength band of light is passed and slantingly incidented onto the fluorescent probe applied to the DNA chip.

Of the light emitted from the fluorescent probe, only light corresponding to the emission wavelength of the fluorescent probe is filtered by the reflection light adjusting part, and image-processed at the CCD camera several times. The overlapped images are inputted into the image reading part, so that a user can diagnose a disease.

Brief Description of the Drawings

The foregoing and other objects, features and advantages of the present invention will become more apparent from the following detailed description when taken in conjunction with the accompanying drawings in which: FIGs. 1 and 2 are schematic constructional views illustrating a general scanner;

FIG. 3 shows a construction of the conventional DNA detector; FIG. 4 shows a block diagram of a DNA detector according to the present invention;

FIG. 5 shows a block diagram of a detector having a lamp as a light source in accordance with a first embodiment of the present invention; FIG. 6 shows a construction of a detector having a halogen lamp;

FIG. 7 shows a construction of a detector having a xenon or mercury lamp;

FIG. 8 shows a construction of a detector having a light emitting diode;

FIG. 9 shows a block diagram of a detector having a laser beam as a light source in accordance with a second embodiment of the present invention; FIG. 10 shows a construction of a detector of a linear scan control mode;

FIG. 11 is a timing chart showing a control mode of a CCD camera by a linear scan control mode;

FIG. 12 shows a construction of a detector in which a scan mode is a total internal reflection mode; FIG. 13 is an operational view explaining how a path of a laser beam is changed by a prism; and

FIG. 14 is an operational view showing a phenomenon in which a laser beam is subjected to total internal reflection on a slide.

- Description of reference numerals for important parts of the drawings - 11 : ELECTRIC POWER SOURCE PART

12 : LIGHT SOURCE PART

13 : INCIDENT LIGHT ADJUSTING PART

14 : SLIDE

15 : EXAMINATION AREA 16 : REFLECTION LIGHT ADJUSTING PART

17 : CCD CAMERA

18 : IMAGE READING PART

19 : TRANSPOT MECHANISM

Best Mode for Carrying Out the Invention

Reference will now be made in detail to the preferred embodiments of the present invention. FIG. 4 shows a block diagram of a DNA detector according to the present invention.

As shown in FIG. 4, a light source part 12 receives electric energy from an electric power source part 11 to emit light. The light emitted from the light source part 12 is incidented toward an incident light adjusting part 13, which removes light components having a wavelength not in the desired wavelength band. Light components having the desired wavelength are incidented toward at least one examination area 15 of a slide 14 at a predetermined acute angle, for example, at about 45 degrees. Of course, at least one DNA chip covered with a fluorescent probe is dotted within the examination area 15.

Meanwhile, the position of the slide 14 is controlled by a transport device 19, for example, an X-Y stage which is operated in a rack-pinion mode or in a ball-screw mode. Here, the number of the DNA chips on the slide 14 may be from one to eight, and each DNA chip has a size of lcπf or less. When the light is scanned on the examination area 15, the scanned area is about 1cm². Thus, one examination area 15 can be read by a single scanning operation. Consequently, when reading of any one of the examination areas 15 is completed, the neighboring one 15 has only to be arranged at a scanning position, so that there is no need to precisely control the transport device as in the art. For this reason, it does not matter to use the transport device 19 which is manually operated in a simple manner.

Light is emitted again from the fluorescent probe onto which original light is incidented. Among the emitted light, some corresponding to an emission wavelength of the fluorescent probe are filtered by a reflection light adjusting part 16. The filtered light is converted into an image by a CCD camera 17. The CCD camera 17 photographs any one of examination areas 15 several times to form an overlapped image. This overlapped image is analyzed and read at an image reading part 18. Looking at this analyzed and read image, a user gives a diagnosis of a disease.

The image reading part 18 processes an image obtained from the light detecting part 17 to obtain a clearer image in the following two methods. One is an image summing method in which, in case that an image obtained from the light detecting part

17 is not clear, the image is sensed by the predetermined number of times, and then the sensed images are all summed up. The other is a band type of thresholding method for removing a portion beyond an eyesight range of a human being and a noise portion.

Here, the reason why incident light is slantingly incidented onto the slide 14 without being parallel to the reflected light is as follows. When the incident light is parallel to the reflected light, an intensity of light is enhanced and thus a quality of image generally gets better. However, due to the enhanced intensity, various wavelengths of light are directly incidented into the CCD camera 17, and this makes it rather more difficult to obtain a desired image.

Hereinafter, a detailed description will be made regarding constituents of each part modified according to the type of light source.

First Embodiment

FIG. 5 shows a block diagram of a detector having a lamp as a light source in accordance with a first embodiment of the present invention. FIG. 6 shows a construction of a detector having a halogen lamp. FIG. 7 shows a construction of a detector having a xenon or mercury lamp. FIG. 8 shows a construction of a detector having a light emitting diode.

In the case of having a lamp as a light source, components of each part are shown in FIG. 5. As shown in FIG. 5, the light source part 12 is provided with a lamp 12a as a light source. Even though it is shown in FIG. 5 that the light source part 12 is provided with a condenser 12b for condensing light and a heat sinker 12c for discharging heat of light, these condenser 12b and heat sinker 12c are optionally used according to the type of the lamp 12a. This will be described below.

An incident light adjusting part 13 includes a light adjusting lens unit 13a for adjusting a quantity and a focus distance of light emitted from the lamp 12a, and a light filter 13b as a band-pass filter for passing only a particular wavelength of light.

Meanwhile, the light adjusting lens unit 13a and the light filter 13b are not fixedly arranged in the order as in FIG. 5, but changed according to the type of the lamp 12a.

Light emitted from a fluorescent probe is incidented into a reflection light adjusting part 16, which includes a light filter 16a for passing only light corresponding to an emission wavelength of the fluorescent probe, and a light adjusting lens unit 16b for collecting and condensing light passing through the light filter 16a.

FIG. 6 shows a construction of a detector having a halogen lamp 12a-l as a lamp 12b. As shown, the halogen lamp 12a-l supplied with electric energy from an electric power source part 1 la is slantingly arranged at an acute angle with respect to a surface of a slide 14. A hemispherical light reflector 12b-l as a condenser 12b for condensing light is arranged around the halogen lamp 12a-l. In front of the halogen lamp 12a-l, a light adjusting lens unit 13a and a light filter 13b are arranged in that order. The light filter 16a and light adjusting lens unit 16b(that is, the reflection light adjusting part 16) are sequentially arranged on a vertical upper portion of the slide 14. A CCD camera 17 is arranged on the upper portion of the reflection light adjusting part 16.

Even though not shown in FIG. 6, it is preferred that a shielding chamber (not shown) for isolating the upper area of the slide 14 from an exterior is provided in order to increase the contrast of image photographed at the CCD camera 17. The shielding chamber prevents external light from entering the upper area of the slide 14, and thereby it is possible to prevent the contrast of image from being decreased due to an interference of light.

FIG. 7 shows a construction of a detector to which a xenon lamp or a mercury lamp 12a-2 as the lamp 12a is employed. Because a life span of the xenon or mercury lamp 12a-2 is greatly dependent on the supply safety of electric power, a constant voltage retaining type of electric power source part 1 lb is used as the electric power source part 11. The hemielliptical light reflector 12b-2 is employed as the condenser. An arrangement direction of the xenon or mercury lamp 12a-2 is the same as that of the foregoing halogen lamp 12a-l. The other components are similar to the construction of the detector shown in

FIG. 6. However, an arrangement sequence of the light filter 13b and light adjusting lens unit 13a is different from that of the detector of FIG. 6. To be more specific, the light filter 13b is arranged directly in front of the xenon or mercury lamp 12a-2, and the light adjusting lens unit 13a is arranged next to the light filter 13b. . FIG. 8 shows a construction of a detector having a light emitting diode 12a-3 as the lamp 12a. As shown, in front of the light emitting diode 12a-3 arranged in the same direction as the halogen lamp 12a-l, the light filter 13b and the light adjusting lens unit 13a are sequentially arranged. The other components are the same as in the scanner shown in FIGs. 6 and 7, and thus they will not be described repeatedly.

Meanwhile, the number of the light emitting diodes 12a-3 is determined by a quantity of light required in the whole system of the detector. Because the light emitting diode has very various and many types and sizes, combination of the light emitting diodes 12a-3 allows them to be variously arranged.

Second Embodiment

FIG. 9 shows a block diagram of a detector having a laser beam as a light source in accordance with a second embodiment of the present invention. FIG. 10 shows a construction of a detector of a linear scan control mode. FIG. 11 is a timing chart showing a control mode of a CCD camera by the linear scan control mode. FIG. 12 shows a construction of a detector in which a scan mode is a total internal reflection mode. FIG. 13 is an operational view explaining how a path of a laser beam is changed by a prism. FIG. 14 is an operational view showing a phenomenon in which a laser beam is subjected to total internal reflection on a slide.

FIG. 9 shows a block diagram of a detector to which a laser beam is employed as a light source instead of a lamp. As shown, a light source part 12 includes a laser beam scan section 12d and a light modifier 12e for modifying the laser beam in a proper form. Here, the light modifier 12e is applied only to a scanner of a linear scan control mode. However, the light modifier 12e is an unnecessary component when a laser beam is subjected to total internal reflection because the laser beam takes a dot form.

An incident light adjusting part 13 includes a light adjusting lens unit 13d for controlling a direction of the laser beam, a light reflector 13c for changing a path of the laser beam, and a rotational driver 13e for rotating the light reflector 13c or the light adjusting lens unit 13d. Meanwhile, it is natural that the light adjusting lens unit 13d has variable number or type according to a scanning mode of the laser beam. The other components are the same as those shown in FIG. 5.

FIG. 10 shows a construction of a detector of a linear scan control mode. As shown, the light source part 12 of the scanner of this control mode includes a light modifier 12e, which includes a collimating lens 12e-l for focusing a laser beam and a cylindrical lens 12e-2 for modifying the focused laser beam taken in a dot form into a linear form. A laser beam scan section (not shown) is arranged in a direction in which the laser beam is perpendicular or vertical to a surface of a slide 14.

An incident light adjusting part 13 includes a polygonal mirror 13c for reflecting laser beam incidented in a vertical downward direction to be incidented onto the slide 14 at a predetermined acute angle, and a rotational driver (not shown) for rotating the polygonal mirror 13c to continuously vary an incident position of the laser beam. That is, in FIG. 10, the incident position of the laser beam is continuously varied from a dot line to a solid line by the rotating polygonal mirror 13c, thus taking one surface form. Meanwhile, in this embodiment, a hexagonal mirror is used as the polygonal mirror 13c.

Here, the polygonal mirror 13c has a width equal to a length of the laser beam as a linear light source. The laser beam has a width of tens through hundreds of μm, and reciprocates an examination area at a high speed in case that the laser beam is incidented onto the slide 14 by the polygonal mirror 13c rotating at a high speed. Thus, it is possible not only to acquire more quantity of light than a surface light source mode in terms of a scanning area, but also to obtain an almost same image as the surface light source mode due to an after-image effect in the CCD camera 17. To be more specific with reference to FIG. 11, a time which the hexagonal mirror 13c needs to rotate a 1/6 turn is tl. During that time, a linear laser beam from the incident light adjusting part 13 moves from a position XI to X2 within the examination area 15, and then returns the position XI again. Therefore, an almost same effect as to scan all the examination area 15 in the surface light source mode can be accomplished.

Meanwhile, the other components are substantially same as those of the foregoing scanner. Thus, herein no description will be repeatedly made regarding those components.

FIG. 12 shows a construction of a scanner in which a laser beam incidented onto a slide is subjected to total internal reflection. As shown, this scanner is not provided with a light modifier because a laser beam must be incidented in a dot form. That is, a laser beam cast from a laser beam scan section 12d, which is arranged perpendicular to the surface of the slide 14, is directly incidented into the incident light adjusting part 13 without passing through the light modifier. The incident light adjusting part 13 includes a prism 13d-l for changing a path of the laser beam, a rotational driver 13e as a DC motor for rotating the prism 13d-l, a cylindrical lens 13d- 2 for adjusting a focus of the laser beam, and a light reflector 13c for slantingly reflecting the laser beam onto the slide 14. FIG. 13 shows how the path of the laser beam is changed by the rotating prism 13d-l .

The laser beam, which is reflected from the light reflector 13c in a dot form, is slantingly incidented into the slide 14 as shown in FIG. 14. Here, the incident angle is determined at such an angle that total internal reflection occurs in the slide 14. Consequently, the laser beam incidented onto the slide 14 in a dot form is represented into one linear light source according to an incident direction. Thus, a linear light source effect as mentioned above can be obtained here.

Meanwhile, the other components are similar to those of the detector shown in FIG. 10. Thus, those components will be no longer described repeatedly.

Industrial Applicability

As can be seen from the foregoing, according to the present invention, a price of the detector can be decreased owing to an inexpensive CCD camera without using a photo multiplier tube (PMT). Further, because a transport device with a simple construction is used, this leads to a price saving. In particular, because components such as a concave lens are reduced compared with the prior art, the price of the detector can be significantly decreased in the several aspects.

Further, the detector of the present invention has advantages in that a general user can manipulate it with ease and can directly check the diagnosed result without an expert. While this invention has been described in connection with what is presently considered to be the most practical and preferred embodiment, it is to be understood that the invention is not limited to the disclosed embodiment and the drawings, but, on the contrary, it is intended to cover various modifications and variations within the spirit and scope of the invention.

Claims

What is Claimed is:

1. A DNA detector used to diagnose a disease, by applying light onto a slide on which at least one DNA chip, as a human tissue sample, covered with a fluorescent probe is dotted and by reading light emitted from the fluorescent probe, the DNA detector comprising: a light source part; an incident light adjusting part for passing, among light emitted from the light source, only a certain wavelength band of light causing the fluorescent probe to emit; a reflection light adjusting part for passing, among light emitted from the emission substance, only light corresponding to an emission wavelength of the fluorescent probe; a CCD camera for forming a plurality of images from light passing through the reflection light adjusting part;

an image reading part for receiving and overlapping the plurality of images outputted from the CCD camera to read the overlapped images; and a transport device for shifting the slide to position another DNA chip at an incident position in order to perform subsequent operation after reading of the image reading part.

2. The DNA detector according to claim 1, wherein the light source part is a lamp arranged in a direction of slantingly incidenting light onto the slide.

3. The DNA detector according to claim 2, wherein the lamp is selected from a group of a halogen lamp, a xenon lamp, a mercury lamp and a light emitting diode.

4. The DNA detector according to claim 1, wherein the light source part is a laser scanning section arranged perpendicular to a surface of the slide.

5. A DNA detector used to diagnose a disease, by applying light onto a slide on which at least one DNA chip, as a human tissue sample, covered with a fluorescent probe is dotted and by reading light emitted from the fluorescent probe, the DNA detector comprising: a lamp slantingly arranged on a surface of the slide at a predetermined acute angle; an incident light adjusting lens unit for adjusting a quantity and a focal distance of light emitted from the lamp; an incident light filter for passing, among light passing tlirough the incident light adjusting lens unit, a wavelength band of light causing the fluorescent probe to emit; a reflected light filter for passing only light corresponding to an emission wavelength of the fluorescent probe; a reflected light adjusting lens unit for collecting and condensing light passing through the reflected light filter; a CCD camera for forming a plurality of images from light passing through the reflected light adjusting lens unit; an image reading part for receiving and overlapping the plurality of images outputted from the CCD camera to read the overlapped images; and a transport device for shifting the slide to position another DNA chip at an incident position in order to perform subsequent operation after reading of the image reading part.

6. The DNA detector according to claim 5, wherein the lamp is a halogen lamp enclosed by a condenser for condensing light in one direction, and wherein the reflected light filter, the reflected light adjusting lens unit and the CCD camera are sequentially arranged on a vertical upper portion of the slide.

7. The DNA detector according to claim 5, wherein the lamp is a xenon lamp or a mercury lamp enclosed by a condenser for condensing light in one direction, and wherein the reflected light filter, the reflected light adjusting lens unit and the CCD camera are arranged on a vertical upper portion of the slide.

8. The DNA detector according to claim 7, wherein an electric power source supplying the xenon lamp or the mercury lamp with an electric energy is a constant voltage retaining type.

9. The DNA detector according to claim 5, wherein the lamp is a light emitting diode, and wherein the reflected light filter, the reflected light adjusting lens unit and the CCD camera are arranged on a vertical upper portion of the slide.

10. A DNA detector used to diagnose a disease, by applying light onto a slide on which at least one DNA chip, as a human tissue sample, covered with a fluorescent probe is dotted and by reading light emitted from the fluorescent probe, the DNA detector comprising: a laser beam scanning section arranged perpendicular to a surface of the slide; a light modifier for changing a laser beam in a dot form cast from the laser beam scanning section into a linear laser beam; a polygonal light reflector for reflecting the linear laser beam to be slantingly incidented onto the slide; a rotational driver for rotating the polygonal light reflector to make the linear laser beam incidented onto the slide to be a surface form;

a reflected light filter for passing only light corresponding to an emission wavelength of the fluorescent probe; a reflected light adjusting lens unit for collecting and condensing light passing through the reflected light filter;

a CCD camera for forming a plurality of images from light passing through the reflected light adjusting lens unit; an image reading part for receiving and overlapping the plurality of images outputted from the CCD camera to read the overlapped images; and a transport device for shifting the slide to position another DNA chip at an incident position in order to perform subsequent operation after reading of the image reading part.

11. The DNA detector according to claim 10, wherein the light modifier comprises a collimating lens for adjusting a focus of the laser beam in a dot form, and a cylindrical lens for changing the laser beam in a dot form into a linear laser beam.

12. The DNA detector according to claim 10, wherein the polygonal light reflector is in a hexagonal shape.

13. A DNA detector used to diagnose a disease, by applying light onto a slide on which at least one DNA chip, as a human tissue sample, covered with a fluorescent probe is dotted and by reading light emitted from the fluorescent probe, the DNA detector comprising:

a laser beam scanning section arranged perpendicular to a surface of the slide; a light adjusting lens unit for changing a path of a laser beam in a dot form cast from the laser beam scanning section and for adjusting a focus of the laser beam; a light reflector arranged at an angle, the angle causing the laser beam in a dot form passing through the light adjusting lens unit to be slantingly incidented onto the slide and causing the laser beam in a dot form incidented onto the slide to be subjected to total internal reflection in the slide; a reflected light filter for passing only light corresponding to an emission wavelength of the fluorescent probe; a reflected light adjusting lens unit for collecting and condensing light passing through the reflected light filter; a CCD camera for forming a plurality of images from light passing through the reflected light adjusting lens unit; an image reading part for receiving and overlapping the plurality of images outputted from the CCD camera to read the overlapped images; and a transport device for shifting the slide to position another DNA chip at an incident position in order to perform subsequent operation after reading of the image reading part.

14. The DNA detector according to claim 13, wherein the light adjusting lens unit comprises:

a prism for changing a path of the laser beam; a rotational driver for rotating the prism; and a cylindrical lens for adjusting a focus of the laser beam.

15. A DNA detector used to diagnose a disease, by applying light onto a slide on which at least one DNA chip, as a human tissue sample, covered with a fluorescent probe is dotted and by reading light emitted from the fluorescent probe, the DNA detector comprising: a halogen lamp slantingly arranged at a predetermined acute angle with respect to a surface of the slide; a condenser arranged to enclose the halogen lamp, for condensing light emitted from the halogen lamp in one direction;

a heat sinker arranged in front of the halogen lamp, for discharging a hot heat of the light;

an incident light adjusting lens unit for adjusting a quantity and a focal distance of light passing through the heat sinker; an incident light filter for passing, among light passing through the incident light adjusting lens unit, a wavelength band of light causing the fluorescent probe to emit; a reflected light filter arranged on a vertical upper portion of the slide, for passing only light corresponding to an emission wavelength of the fluorescent probe;

a reflected light adjusting lens unit for collecting and condensing light passing through the reflected light filter; a CCD camera for forming a plurality of images from light passing through the reflected light adjusting lens unit; a shielding chamber for isolating an upper area of the slide from an exterior in order to prevent a contrast of image photographed by the CCD camera from being decreased by interference of the image with external light; an image reading part for receiving and overlapping the plurality of images outputted from the CCD camera to read the overlapped images; and

a transport device for shifting the slide to position another DNA chip at an incident position in order to perform subsequent operation after reading of the image reading part.