WO2004039499A3 - Method for loading and unloading macro-molecules from microfluidic devices - Google Patents

Method for loading and unloading macro-molecules from microfluidic devices Download PDF

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Publication number
WO2004039499A3
WO2004039499A3 PCT/US2003/034865 US0334865W WO2004039499A3 WO 2004039499 A3 WO2004039499 A3 WO 2004039499A3 US 0334865 W US0334865 W US 0334865W WO 2004039499 A3 WO2004039499 A3 WO 2004039499A3
Authority
WO
WIPO (PCT)
Prior art keywords
unloading
macro
molecules
loading
microfluidic devices
Prior art date
Application number
PCT/US2003/034865
Other languages
French (fr)
Other versions
WO2004039499A2 (en
Inventor
Lotien Richard Huang
James Christopher Sturm
Original Assignee
Univ Princeton
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Princeton filed Critical Univ Princeton
Priority to AU2003287456A priority Critical patent/AU2003287456A1/en
Publication of WO2004039499A2 publication Critical patent/WO2004039499A2/en
Publication of WO2004039499A3 publication Critical patent/WO2004039499A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/069—Absorbents; Gels to retain a fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44743—Introducing samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention provides an integrated microfluidic device comprising a sample chamber and a micro-fluidic channel, wherein the sample chamber contains two or more electrodes capable of generating an electric field in the sample chamber. The two or more electrodes create electric fields for sample elution, transfer, and unloading.
PCT/US2003/034865 2002-10-30 2003-10-30 Method for loading and unloading macro-molecules from microfluidic devices WO2004039499A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003287456A AU2003287456A1 (en) 2002-10-30 2003-10-30 Method for loading and unloading macro-molecules from microfluidic devices

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US42230902P 2002-10-30 2002-10-30
US60/422,309 2002-10-30

Publications (2)

Publication Number Publication Date
WO2004039499A2 WO2004039499A2 (en) 2004-05-13
WO2004039499A3 true WO2004039499A3 (en) 2004-11-18

Family

ID=32230339

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/034865 WO2004039499A2 (en) 2002-10-30 2003-10-30 Method for loading and unloading macro-molecules from microfluidic devices

Country Status (2)

Country Link
AU (1) AU2003287456A1 (en)
WO (1) WO2004039499A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0818609D0 (en) 2008-10-10 2008-11-19 Univ Hull apparatus and method
WO2017041809A1 (en) * 2015-09-07 2017-03-16 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Method and apparatus for detecting particles, like biological macromolecules or nanoparticles
US11072827B2 (en) * 2018-01-08 2021-07-27 Quantum-Si Incorporated System and methods for electrokinetic loading of sub-micron-scale reaction chambers

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203683B1 (en) * 1998-11-09 2001-03-20 Princeton University Electrodynamically focused thermal cycling device

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6203683B1 (en) * 1998-11-09 2001-03-20 Princeton University Electrodynamically focused thermal cycling device

Also Published As

Publication number Publication date
AU2003287456A8 (en) 2004-05-25
AU2003287456A1 (en) 2004-05-25
WO2004039499A2 (en) 2004-05-13

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