WO2004027072A3 - Method for making recombinant proteins - Google Patents

Method for making recombinant proteins Download PDF

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Publication number
WO2004027072A3
WO2004027072A3 PCT/DK2003/000607 DK0300607W WO2004027072A3 WO 2004027072 A3 WO2004027072 A3 WO 2004027072A3 DK 0300607 W DK0300607 W DK 0300607W WO 2004027072 A3 WO2004027072 A3 WO 2004027072A3
Authority
WO
WIPO (PCT)
Prior art keywords
factor vii
recombinant proteins
expression
vii
polypeptide
Prior art date
Application number
PCT/DK2003/000607
Other languages
French (fr)
Other versions
WO2004027072A2 (en
Inventor
Ruth Buemann Simesen
Jan Eric Jensen
Anette Amstrup Pedersen
Steffen Faisst
Dietmar Weilguny
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Priority to AU2003263161A priority Critical patent/AU2003263161A1/en
Priority to EP03797190A priority patent/EP1554389A2/en
Publication of WO2004027072A2 publication Critical patent/WO2004027072A2/en
Publication of WO2004027072A3 publication Critical patent/WO2004027072A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6437—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21021—Coagulation factor VIIa (3.4.21.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to scaffold/matrix attachment regions isolated from hamster cells and their use for the industrial production of recombinant proteins and polypep-tides. In one embodiment the invention is related to a method for production of Factor VII polypeptides or Factor VII-related polypeptides comprising a) transfecting a mammalian cell with an expression vector comprising a nucleic acid molecule encoding Factor VII or a Factor VII-related polypeptide, expression control regions operatively linked to thereto and at least one S/MAR element; b) culturing the transfected cell under conditions for expression of Fac-tor VII or a Factor VII-related polypeptide and c) isolating the expressed polypeptide by suit-able means.
PCT/DK2003/000607 2002-09-20 2003-09-19 Method for making recombinant proteins WO2004027072A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2003263161A AU2003263161A1 (en) 2002-09-20 2003-09-19 Method for making recombinant proteins
EP03797190A EP1554389A2 (en) 2002-09-20 2003-09-19 Method for making recombinant proteins

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DKPA200201384 2002-09-20
DKPA200201384 2002-09-20
US41656602P 2002-10-07 2002-10-07
US60/416,566 2002-10-07

Publications (2)

Publication Number Publication Date
WO2004027072A2 WO2004027072A2 (en) 2004-04-01
WO2004027072A3 true WO2004027072A3 (en) 2004-05-13

Family

ID=32031674

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2003/000607 WO2004027072A2 (en) 2002-09-20 2003-09-19 Method for making recombinant proteins

Country Status (3)

Country Link
EP (1) EP1554389A2 (en)
AU (1) AU2003263161A1 (en)
WO (1) WO2004027072A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004056986A2 (en) 2002-12-20 2004-07-08 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
AU2005300503B2 (en) 2004-11-08 2010-12-16 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US7968700B2 (en) 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof
EP3382029B1 (en) * 2017-03-31 2021-12-08 Toto Ltd. Recombinant mammalian cells and method for producing substance of interest

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996019573A1 (en) * 1994-12-19 1996-06-27 Cangene Corporation Recombinant dna molecules and expression vectors for erythropoietin
EP0999280A2 (en) * 1998-10-17 2000-05-10 MultiGene Biotech GmbH Episomally replicating vector, its preparation and use
WO2002014525A2 (en) * 2000-07-29 2002-02-21 Mogam Biotechnology Research Institute Expression vector using for animal cell

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996019573A1 (en) * 1994-12-19 1996-06-27 Cangene Corporation Recombinant dna molecules and expression vectors for erythropoietin
EP0999280A2 (en) * 1998-10-17 2000-05-10 MultiGene Biotech GmbH Episomally replicating vector, its preparation and use
WO2002014525A2 (en) * 2000-07-29 2002-02-21 Mogam Biotechnology Research Institute Expression vector using for animal cell

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SINGH GAUTAM B ET AL: "Mathematical model to predict regions of chromatin attachment to the nuclear matrix", NUCLEIC ACIDS RESEARCH, vol. 25, no. 7, 1997, pages 1419 - 1425, XP002273524, ISSN: 0305-1048 *

Also Published As

Publication number Publication date
WO2004027072A2 (en) 2004-04-01
AU2003263161A8 (en) 2004-04-08
EP1554389A2 (en) 2005-07-20
AU2003263161A1 (en) 2004-04-08

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