WO2004011638A3 - Deoxyuridine 5' triphosphate nucleotidohydrolase polypeptides and structures - Google Patents

Deoxyuridine 5' triphosphate nucleotidohydrolase polypeptides and structures Download PDF

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Publication number
WO2004011638A3
WO2004011638A3 PCT/CA2003/001129 CA0301129W WO2004011638A3 WO 2004011638 A3 WO2004011638 A3 WO 2004011638A3 CA 0301129 W CA0301129 W CA 0301129W WO 2004011638 A3 WO2004011638 A3 WO 2004011638A3
Authority
WO
WIPO (PCT)
Prior art keywords
polypeptides
deoxyuridine
structures
triphosphate nucleotidohydrolase
seq
Prior art date
Application number
PCT/CA2003/001129
Other languages
French (fr)
Other versions
WO2004011638A2 (en
Inventor
Aled Edwards
Akil Dharamsi
Masoud Vedadi
Megan Domagala
Kamran Mansoury
Matthew Kimber
Simon Houston
Donald Awrey
Bryan Beattie
Original Assignee
Affinium Pharm Inc
Aled Edwards
Akil Dharamsi
Masoud Vedadi
Megan Domagala
Kamran Mansoury
Matthew Kimber
Simon Houston
Donald Awrey
Bryan Beattie
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/CA2003/000485 external-priority patent/WO2003087354A2/en
Application filed by Affinium Pharm Inc, Aled Edwards, Akil Dharamsi, Masoud Vedadi, Megan Domagala, Kamran Mansoury, Matthew Kimber, Simon Houston, Donald Awrey, Bryan Beattie filed Critical Affinium Pharm Inc
Priority to AU2003250684A priority Critical patent/AU2003250684A1/en
Publication of WO2004011638A2 publication Critical patent/WO2004011638A2/en
Publication of WO2004011638A3 publication Critical patent/WO2004011638A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

The present invention relates to novel drug targets for pathogenic bacteria. Accordingly, the invention provides purified proteins comprising the amino acid sequences set forth in SEQ ID NO: 4 and SEQ ID NO: 13. The invention also provides biochemical and biophysical characteristics of the polypeptides of the invention.
PCT/CA2003/001129 2002-07-31 2003-07-31 Deoxyuridine 5' triphosphate nucleotidohydrolase polypeptides and structures WO2004011638A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2003250684A AU2003250684A1 (en) 2002-07-31 2003-07-31 Deoxyuridine 5' triphosphate nucleotidohydrolase polypeptides and structures

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US39997102P 2002-07-31 2002-07-31
US60/399,971 2002-07-31
CAPCT/CA03/00485 2003-04-08
PCT/CA2003/000485 WO2003087354A2 (en) 2002-04-09 2003-04-08 Novel purified polypeptides involved in nucleotide transport and metabolism

Publications (2)

Publication Number Publication Date
WO2004011638A2 WO2004011638A2 (en) 2004-02-05
WO2004011638A3 true WO2004011638A3 (en) 2004-07-01

Family

ID=31189053

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CA2003/001129 WO2004011638A2 (en) 2002-07-31 2003-07-31 Deoxyuridine 5' triphosphate nucleotidohydrolase polypeptides and structures

Country Status (2)

Country Link
AU (1) AU2003250684A1 (en)
WO (1) WO2004011638A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3006055B1 (en) * 2013-05-24 2020-08-14 Commissariat Energie Atomique PROCESS FOR CHARACTERIZING BY TANDEM MASS SPECTROMETRY A BIOLOGICAL SAMPLE
KR102356757B1 (en) * 2013-10-18 2022-02-03 노바셉 이큅먼트 솔루션즈 Purification of proteins

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6356845B1 (en) * 1999-08-04 2002-03-12 Pharmacia & Upjohn Company Crystallization and structure determination of Staphylococcus aureus UDP-N-acetylenolpyruvylglucosamine reductase (S. aureus MurB)
WO2002034771A2 (en) * 2000-10-27 2002-05-02 Chiron Srl Nucleic acids and proteins from streptococcus groups a & b
WO2002077021A2 (en) * 2001-03-27 2002-10-03 Chiron Srl. Streptococcus pneumoniae proteins and nucleic acids

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6356845B1 (en) * 1999-08-04 2002-03-12 Pharmacia & Upjohn Company Crystallization and structure determination of Staphylococcus aureus UDP-N-acetylenolpyruvylglucosamine reductase (S. aureus MurB)
WO2002034771A2 (en) * 2000-10-27 2002-05-02 Chiron Srl Nucleic acids and proteins from streptococcus groups a & b
WO2002077021A2 (en) * 2001-03-27 2002-10-03 Chiron Srl. Streptococcus pneumoniae proteins and nucleic acids

Non-Patent Citations (14)

* Cited by examiner, † Cited by third party
Title
ABOLA E ET AL: "AUTOMATION OF X-RAY CRYSTALLOGRAPHY", NATURE STRUCTURAL BIOLOGY, NEW YORK, NY, US, vol. 7, no. SUPPL, November 2000 (2000-11-01), pages 973 - 977, XP001062873, ISSN: 1072-8368 *
BAGBY S ET AL: "OPTIMIZATION OF PROTEIN SOLUBILITY AND STABILITY FOR PROTEIN NUCLEAR MAGNETIC RESONANCE", METHODS IN ENZYMOLOGY, ACADEMIC PRESS INC, SAN DIEGO, CA, US, vol. 339, 2001, pages 20 - 41, XP001152932, ISSN: 0076-6879 *
BURKHARD P ET AL: "The discovery of steroids and other novel FKBP inhibitors using a molecular docking program.", JOURNAL OF MOLECULAR BIOLOGY. ENGLAND 16 APR 1999, vol. 287, no. 5, 16 April 1999 (1999-04-16), pages 853 - 858, XP002256057, ISSN: 0022-2836 *
CEDERGREN-ZEPPEZAUER E S ET AL: "Crystal structure of a dUTPase.", NATURE. ENGLAND 20 FEB 1992, vol. 355, no. 6362, 20 February 1992 (1992-02-20), pages 740 - 743, XP001155358, ISSN: 0028-0836 *
DATABASE EMBL [online] 7 September 2001 (2001-09-07), XP002262428, retrieved from EBI Database accession no. AE008386 *
DATABASE GSP [online] 2 July 2002 (2002-07-02), XP002262429, retrieved from EBI Database accession no. ABP30148 *
DATABASE PATENT_PRT [online] 29 November 2002 (2002-11-29), XP002262430, retrieved from EBI Database accession no. AX571540 *
DATABASE SWALL [online] 1 October 2001 (2001-10-01), XP002256059, retrieved from EBI Database accession no. Q97TB9 *
GONZÁLEZ A ET AL: "Atomic resolution structure of Escherichia coli dUTPase determined ab initio.", ACTA CRYSTALLOGRAPHICA. SECTION D, BIOLOGICAL CRYSTALLOGRAPHY. DENMARK JUN 2001, vol. 57, no. Pt 6, June 2001 (2001-06-01), pages 767 - 774, XP009018221, ISSN: 0907-4449 *
HOSKINS J ET AL: "Genome of the Bacterium Streptococcus pneumoniae Strain R6", JOURNAL OF BACTERIOLOGY, WASHINGTON, DC, US, vol. 183, no. 19, October 2001 (2001-10-01), pages 5709 - 5717, XP002231307, ISSN: 0021-9193 *
KRAFT P ET AL: "Infrared, surface-assisted laser desorption ionization mass spectrometry on frozen aqueous solutions of proteins and peptides using suspensions of organic solids", JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, ELSEVIER SCIENCE INC., NEW YORK, NY, US, vol. 9, no. 9, September 1998 (1998-09-01), pages 912 - 924, XP004149186, ISSN: 1044-0305 *
MOORE J M: "NMR techniques for characterization of ligand binding: utility for lead generation and optimization in drug discovery.", BIOPOLYMERS. UNITED STATES 1999, vol. 51, no. 3, 1999, pages 221 - 243, XP002256058, ISSN: 0006-3525 *
NORD^A J ET AL: "The C-terminus of dUTPase: observation on flexibility using NMR", FEBS LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 492, no. 3, 16 March 2001 (2001-03-16), pages 228 - 232, XP004257368, ISSN: 0014-5793 *
TETTELIN H ET AL: "Complete genome sequence of a virulent isolate of Streptococcus pneumoniae", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 293, no. 5529, 2001, pages 498 - 506, XP002218261, ISSN: 0036-8075 *

Also Published As

Publication number Publication date
WO2004011638A2 (en) 2004-02-05
AU2003250684A8 (en) 2004-02-16
AU2003250684A1 (en) 2004-02-16

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