WO2002052926A3 - Methods for generating doubled haploid maize plants - Google Patents

Methods for generating doubled haploid maize plants Download PDF

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Publication number
WO2002052926A3
WO2002052926A3 PCT/US2002/000327 US0200327W WO02052926A3 WO 2002052926 A3 WO2002052926 A3 WO 2002052926A3 US 0200327 W US0200327 W US 0200327W WO 02052926 A3 WO02052926 A3 WO 02052926A3
Authority
WO
WIPO (PCT)
Prior art keywords
microspores
maize
temperature
methods
regenerative
Prior art date
Application number
PCT/US2002/000327
Other languages
French (fr)
Other versions
WO2002052926A2 (en
Inventor
Yuanming Zheng
Calvin F Konzak
Yujia Weng
Sahibzada Rafiullah
Original Assignee
Northwest Plant Breeding Co
Yuanming Zheng
Calvin F Konzak
Yujia Weng
Sahibzada Rafiullah
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Northwest Plant Breeding Co, Yuanming Zheng, Calvin F Konzak, Yujia Weng, Sahibzada Rafiullah filed Critical Northwest Plant Breeding Co
Publication of WO2002052926A2 publication Critical patent/WO2002052926A2/en
Publication of WO2002052926A3 publication Critical patent/WO2002052926A3/en

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08—Methods for producing changes in chromosome number
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants

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  • Life Sciences & Earth Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

In one aspect, the present invention provides methods for generating doubled haploid and/or haploid maize plants from microspores. The methods of this aspect of the invention include the steps of: (a) selecting maize plant material comprising maize microspores at a developmental stage amenable to androgenic induction; (b) incubating the microspores in incubation medium at a temperature and osmolarity effective to induce androgenesis to obtain temperature-treated microspores; (c) isolating the temperature-treated microspores; (d) cultivating the isolated, temperature-treated microspores in cultivation medium with either at least one live plant ovary and/or ovary-conditioned medium to produce regenerative maize tissue, wherein the cultivation medium has an osmolarity between about 300 mOsm and about 500 mOsm and comprises at least one cytokinin and at least one auxin; and (e) regenerating maize plants from the regenerative maize tissue. The present invention also provides methods for producing regenerative maize tissue from maize microspores.
PCT/US2002/000327 2001-01-05 2002-01-02 Methods for generating doubled haploid maize plants WO2002052926A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26002801P 2001-01-05 2001-01-05
US60/260,028 2001-01-05

Publications (2)

Publication Number Publication Date
WO2002052926A2 WO2002052926A2 (en) 2002-07-11
WO2002052926A3 true WO2002052926A3 (en) 2003-04-03

Family

ID=22987517

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/000327 WO2002052926A2 (en) 2001-01-05 2002-01-02 Methods for generating doubled haploid maize plants

Country Status (1)

Country Link
WO (1) WO2002052926A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103444525B (en) * 2013-08-15 2014-12-24 广东省农业科学院作物研究所 Hormone-free corn callus regeneration method
WO2015043621A1 (en) * 2013-09-24 2015-04-02 Stichting Dienst Landbouwkundig Onderzoek Haploid embryogenesis
WO2021221743A1 (en) * 2020-05-01 2021-11-04 Monsanto Technology Llc Methods for improved microscope embryogenesis and production of doubled haploid microspore-derived embryos
CN114793792B (en) * 2022-04-24 2023-06-20 河南科技学院 Method for restoring pollen fertility of different fertility populations of BNS series temperature-sensitive genic male sterile wheat

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009450A2 (en) * 1989-02-17 1990-08-23 United Agriseeds, Inc. Method for increasing the ability of maize to undergo androgenesis, and products produced therefrom
EP0636310A1 (en) * 1993-07-06 1995-02-01 Sandoz Ltd. Doubled haploids
WO2001014518A2 (en) * 1999-08-26 2001-03-01 Northwest Plant Breeding Co. Methods for generating doubled haploid plants
WO2001041557A2 (en) * 1999-12-10 2001-06-14 University Of Guelph Embryogenesis and plant regeneration from microspores

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990009450A2 (en) * 1989-02-17 1990-08-23 United Agriseeds, Inc. Method for increasing the ability of maize to undergo androgenesis, and products produced therefrom
EP0636310A1 (en) * 1993-07-06 1995-02-01 Sandoz Ltd. Doubled haploids
WO2001014518A2 (en) * 1999-08-26 2001-03-01 Northwest Plant Breeding Co. Methods for generating doubled haploid plants
WO2001041557A2 (en) * 1999-12-10 2001-06-14 University Of Guelph Embryogenesis and plant regeneration from microspores

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BARLOY D ET AL: "Improvement of regeneration ability of androgenetic embryos by early anther transfer in maize.", PLANT CELL TISSUE AND ORGAN CULTURE, vol. 33, no. 1, 1993, pages 45 - 50, XP009001911, ISSN: 0167-6857 *
BRUINS MARCEL B M ET AL: "Isolated microspore culture in wheat (Triticum aestivum L.): The effect of co-culture of wheat or barley ovaries on embryogenesis.", CEREAL RESEARCH COMMUNICATIONS, vol. 24, no. 4, 1996, pages 401 - 408, XP001120752, ISSN: 0133-3720 *
GAILLARD A ET AL: "OPTIMIZATION OF MAIZE MICROSPORE ISOLATION AND CULTURE CONDITIONS FOR RELIABLE PLANT REGENERATION", PLANT CELL REPORTS, vol. 10, no. 2, 1991, pages 55 - 58, XP009001910, ISSN: 0721-7714 *
HU T ET AL: "Improvement of isolated microspore culture of wheat (Triticum aestivum L.) through ovary co-culture.", PLANT CELL REPORTS, vol. 16, no. 8, 1997, pages 520 - 525, XP002223011, ISSN: 0721-7714 *
PUOLIMATKA MATTI ET AL: "Effect of ovary co-cultivation and culture medium on embryogenesis of directly isolated microspores of wheat.", CEREAL RESEARCH COMMUNICATIONS, vol. 24, no. 4, 1996, pages 393 - 400, XP001120753, ISSN: 0133-3720 *
ZHENG M Y ET AL: "The effect of ovary-conditioned medium on microspore embryogenesis in common wheat (Triticum aestivum L.).", PLANT CELL REPORTS, vol. 20, no. 9, February 2002 (2002-02-01), pages 802 - 807, XP002223012, ISSN: 0721-7714 *

Also Published As

Publication number Publication date
WO2002052926A2 (en) 2002-07-11

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