WO2002046468A3 - Method for maldi-tof-ms analysis and/or sequencing of oligonucleotides - Google Patents

Method for maldi-tof-ms analysis and/or sequencing of oligonucleotides Download PDF

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Publication number
WO2002046468A3
WO2002046468A3 PCT/JP2001/010689 JP0110689W WO0246468A3 WO 2002046468 A3 WO2002046468 A3 WO 2002046468A3 JP 0110689 W JP0110689 W JP 0110689W WO 0246468 A3 WO0246468 A3 WO 0246468A3
Authority
WO
WIPO (PCT)
Prior art keywords
maldi
tof
sequencing
analysis
oligonucleotides
Prior art date
Application number
PCT/JP2001/010689
Other languages
French (fr)
Other versions
WO2002046468A2 (en
Inventor
Yoshihide Hayashizaki
Tetsuyoshi Ono
Original Assignee
Riken
Yoshihide Hayashizaki
Tetsuyoshi Ono
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Riken, Yoshihide Hayashizaki, Tetsuyoshi Ono filed Critical Riken
Priority to EP01999673A priority Critical patent/EP1364058A2/en
Priority to US10/433,899 priority patent/US20040126772A1/en
Priority to CA002430648A priority patent/CA2430648A1/en
Priority to JP2002548185A priority patent/JP2004518420A/en
Publication of WO2002046468A2 publication Critical patent/WO2002046468A2/en
Publication of WO2002046468A3 publication Critical patent/WO2002046468A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6872—Methods for sequencing involving mass spectrometry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/31—Chemical structure of the backbone
    • C12N2310/315—Phosphorothioates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/32—Chemical structure of the sugar

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  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present invention is related to a method for MALDI-TOF-MS analysis and/or sequencing of oligoribonucleotides. The present invention is also related to a method for determining the DNA nucleotide sequence using MALDI-TOF-MS, and a method for determining polymorphism using MALDI-TOF-MS. The present invention provides a kit for analysing and/or sequencing a DNA template or a RNA transcription product and/or for determining polymorphism by MALDI-TOF-MS.
PCT/JP2001/010689 2000-12-08 2001-12-06 Method for maldi-tof-ms analysis and/or sequencing of oligonucleotides WO2002046468A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP01999673A EP1364058A2 (en) 2000-12-08 2001-12-06 Method for maldi-tof-ms analysis and/or sequencing of oligonucleotides
US10/433,899 US20040126772A1 (en) 2001-12-06 2001-12-06 Method for maldi-tof-ms analysis and/or sequencing of oligonucleotides
CA002430648A CA2430648A1 (en) 2000-12-08 2001-12-06 Method for maldi-tof-ms analysis and/or sequencing of oligonucleotides
JP2002548185A JP2004518420A (en) 2000-12-08 2001-12-06 MALDI-TOF-MS analysis and / or sequencing of oligoribonucleotides

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25185500P 2000-12-08 2000-12-08
US60/251,855 2000-12-08

Publications (2)

Publication Number Publication Date
WO2002046468A2 WO2002046468A2 (en) 2002-06-13
WO2002046468A3 true WO2002046468A3 (en) 2003-09-12

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2001/010689 WO2002046468A2 (en) 2000-12-08 2001-12-06 Method for maldi-tof-ms analysis and/or sequencing of oligonucleotides

Country Status (4)

Country Link
EP (1) EP1364058A2 (en)
JP (1) JP2004518420A (en)
CA (1) CA2430648A1 (en)
WO (1) WO2002046468A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006038865A1 (en) * 2004-10-01 2006-04-13 Betagenon Ab Nucleotide derivatives for the treatment of type 2 diabetes and other disorders
JP5184621B2 (en) 2007-04-18 2013-04-17 エフ．ホフマン−ラ ロシュ アーゲー Nucleotide having α-phosphate mimetic
JP5036042B2 (en) * 2007-05-28 2012-09-26 株式会社島津製作所 Mass spectrometry sample preparation method, ribonucleic acid ionization method, ribonucleic acid mass spectrometry method, and cell-derived low molecular ribonucleic acid mass spectrometry method
JP5569264B2 (en) * 2010-08-31 2014-08-13 株式会社島津製作所 RNA sequencing by ion source cleavage using matrix-assisted laser desorption / ionization time-of-flight mass spectrometer
AP3584A (en) * 2010-09-22 2016-02-09 Alios Biopharma Inc Substituted nucleotide analogs
WO2023234372A1 (en) * 2022-05-31 2023-12-07 株式会社島津製作所 Nucleic acid mass spectrometry method
WO2023234373A1 (en) * 2022-05-31 2023-12-07 株式会社島津製作所 Nucleic-acid structural analysis method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999031278A1 (en) * 1997-12-15 1999-06-24 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999031278A1 (en) * 1997-12-15 1999-06-24 Sequenom, Inc. Mass spectrometric methods for sequencing nucleic acids

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
KIRPEKAR FINN ET AL: "RNA fragmentation studied in a matrix-assisted laser desorption/ionisation tandem quadrupole/orthogonal time-of-flight mass spectrometer.", RAPID COMMUNICATIONS IN MASS SPECTROMETRY, vol. 15, no. 1, 30 November 2000 (2000-11-30), pages 8 - 14, XP008013491, ISSN: 0951-4198 *
NORDHOFF E ET AL: "ION STABILITY OF NUCLEIC ACIDS IN INFRARED MATRIX-ASSISTED LASER DISORPTION/IONIZATION MASS SPECTROMETRY", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 21, no. 15, 25 July 1993 (1993-07-25), pages 3347 - 3357, XP000537440, ISSN: 0305-1048 *
SASAKI N ET AL: "TRANSCRIPTIONAL SEQUENCING: A METHOD FOR DNA SEQUENCING USING RNA POLYMERASE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 95, 31 March 1998 (1998-03-31), pages 3455 - 3460, XP002912287, ISSN: 0027-8424 *
SCHUETTE JODI M ET AL: "Sequence analysis of phosphorothioate oligonucleotides via matrix-assisted laser desorption ionization time-of-flight mass spectrometry.", JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, vol. 13, no. 10, 1995, pages 1195 - 1203, XP002234148, ISSN: 0731-7085 *
TANG W ET AL: "Controlling DNA fragmentation in MALDI-MS by chemical modification", ANALYTICAL CHEMISTRY, vol. 69, no. 3, 1997, pages 302 - 312, XP002234146, ISSN: 0003-2700 *
WANG BING H ET AL: "Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of chemically modified oligonucleotides.", ANALYTICAL CHEMISTRY, vol. 66, no. 11, 1994, pages 1918 - 1924, XP002234147, ISSN: 0003-2700 *

Also Published As

Publication number Publication date
JP2004518420A (en) 2004-06-24
WO2002046468A2 (en) 2002-06-13
EP1364058A2 (en) 2003-11-26
CA2430648A1 (en) 2002-06-13

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