WO2002033052A3 - Method for producing vital, biological substitute tissue in vitro using cell cultures and a support - Google Patents

Method for producing vital, biological substitute tissue in vitro using cell cultures and a support Download PDF

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Publication number
WO2002033052A3
WO2002033052A3 PCT/DE2001/003959 DE0103959W WO0233052A3 WO 2002033052 A3 WO2002033052 A3 WO 2002033052A3 DE 0103959 W DE0103959 W DE 0103959W WO 0233052 A3 WO0233052 A3 WO 0233052A3
Authority
WO
WIPO (PCT)
Prior art keywords
support
vitro
cell cultures
cell
tissue
Prior art date
Application number
PCT/DE2001/003959
Other languages
German (de)
French (fr)
Other versions
WO2002033052A2 (en
Inventor
Ralf Sodian
Thees Lembke
Original Assignee
Deutsches Herzzentrum Berlin
Ralf Sodian
Thees Lembke
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Deutsches Herzzentrum Berlin, Ralf Sodian, Thees Lembke filed Critical Deutsches Herzzentrum Berlin
Priority to AU2002221526A priority Critical patent/AU2002221526A1/en
Priority to EP01987795A priority patent/EP1326962A2/en
Publication of WO2002033052A2 publication Critical patent/WO2002033052A2/en
Publication of WO2002033052A3 publication Critical patent/WO2002033052A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture

Abstract

The invention relates to a method for producing vital, biological substitute tissue in vitro using cell cultures and a support. The inventive method is characterized in that a cell-tissue construct produced from the support and the cell cultures is subjected to a changing mechanical stress, especially a combination of a pulsating flow of a cell culture medium and a pulsating movement of the cell-tissue construct.
PCT/DE2001/003959 2000-10-17 2001-10-17 Method for producing vital, biological substitute tissue in vitro using cell cultures and a support WO2002033052A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002221526A AU2002221526A1 (en) 2000-10-17 2001-10-17 Method for producing vital, biological substitute tissue in vitro using cell cultures and a support
EP01987795A EP1326962A2 (en) 2000-10-17 2001-10-17 Method for producing vital, biological substitute tissue in vitro using cell cultures and a support

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10053014.1 2000-10-17
DE10053014A DE10053014A1 (en) 2000-10-17 2000-10-17 In vitro production of substitute patch tissue, for reconstruction of cardio vascular structures, comprises cell culture-tissue structure in bioreactor subjected to shifting pressures and movements

Publications (2)

Publication Number Publication Date
WO2002033052A2 WO2002033052A2 (en) 2002-04-25
WO2002033052A3 true WO2002033052A3 (en) 2002-11-28

Family

ID=7661079

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/003959 WO2002033052A2 (en) 2000-10-17 2001-10-17 Method for producing vital, biological substitute tissue in vitro using cell cultures and a support

Country Status (4)

Country Link
EP (1) EP1326962A2 (en)
AU (1) AU2002221526A1 (en)
DE (1) DE10053014A1 (en)
WO (1) WO2002033052A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8399243B2 (en) 2005-02-17 2013-03-19 Universitaet Zuerich Method of manufacturing a tissue-engineered prosthesis

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10249903B4 (en) * 2002-10-22 2007-10-11 Cytonet Gmbh & Co. Kg Mechanical bioreactor
DE10322024A1 (en) * 2003-05-16 2004-12-02 Symetis Ag Bioreactor for manufacturing a tissue prosthesis, in particular a heart valve
DE102004001225B8 (en) * 2004-01-05 2006-07-27 fzmb Forschungszentrum für Medizintechnik und Biotechnologie e.V. Process for producing three-dimensional, carrier-free fabric structures and fabric structures produced by this process
DE102008050424B4 (en) 2008-10-08 2010-11-25 Universität Leipzig Method and device for the homogeneous distribution of a cellular suspension in porous carrier material for the production of vital biological replacement tissue
DE102009039535B4 (en) * 2009-09-01 2015-08-20 Corlife Ohg Container, apparatus and method for treating tissue under clean room conditions
EP2399986A1 (en) 2010-06-22 2011-12-28 Ludwig-Maximilians-Universität München Bioreactor and method for creating and/or conditioning biological tissues
EP2468846A1 (en) 2010-12-22 2012-06-27 Ludwig-Maximilians-Universität München A method of examining tissue growth and conditioning of cells on a scaffold and a perfusion bioreactor
EP2500410A1 (en) 2011-03-18 2012-09-19 Ludwig-Maximilians-Universität München Bioreactor with mechanical and electrical stimulation means
CZ2012376A3 (en) 2012-06-05 2013-12-18 Institut Klinické A Experimentální Medicíny Process for preparing pericardial prosthesis of cardiac valve, cardiac valve pericardial prosthesis produced in such a manner, device for conditioning and modification of autologous pericardial tissue for pericardial prosthesis of heart valve
IT202000021847A1 (en) * 2020-09-16 2022-03-16 React4Life S R L DEVICE FOR PERFORMING IN VITRO EXPERIMENTS ON BIOLOGICAL MATERIAL
CN113249211A (en) * 2021-05-26 2021-08-13 庆开雄 Cell culture box capable of changing pressure in box and pressure adjusting method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5153136A (en) * 1988-07-22 1992-10-06 Vandenburgh Herman H Apparatus for growing tissue specimens in vitro
US5348879A (en) * 1990-08-24 1994-09-20 The General Hospital Corporation Cell stretching method
WO1997049799A1 (en) * 1996-06-27 1997-12-31 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5153136A (en) * 1988-07-22 1992-10-06 Vandenburgh Herman H Apparatus for growing tissue specimens in vitro
US5348879A (en) * 1990-08-24 1994-09-20 The General Hospital Corporation Cell stretching method
WO1997049799A1 (en) * 1996-06-27 1997-12-31 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HOERSTRUP SIMON P ET AL: "New pulsatile bioreactor for in vitro formation of tissue engineered heart valves.", TISSUE ENGINEERING, vol. 6, no. 1, February 2000 (2000-02-01), pages 75 - 79, XP001040483, ISSN: 1076-3279 *
NIKLASON L E ET AL: "Functional arteries grown in vitro.", SCIENCE (WASHINGTON D C), vol. 284, no. 5413, 16 April 1999 (1999-04-16), pages 489 - 493, XP002200891, ISSN: 0036-8075 *
SODIAN R ET AL: "NEW PULSATILE BIORECTOR FOR FABRICATION OF TISSUE-ENGINEERED PATCHES", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, WILEY, NEW YORK, NY, US, vol. 58, no. 4, 2001, pages 401 - 405, XP001037742, ISSN: 0021-9304 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8399243B2 (en) 2005-02-17 2013-03-19 Universitaet Zuerich Method of manufacturing a tissue-engineered prosthesis

Also Published As

Publication number Publication date
AU2002221526A1 (en) 2002-04-29
WO2002033052A2 (en) 2002-04-25
EP1326962A2 (en) 2003-07-16
DE10053014A1 (en) 2002-04-18

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