WO2001092291A3 - Antibody and t-cell receptor libraries - Google Patents

Antibody and t-cell receptor libraries Download PDF

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Publication number
WO2001092291A3
WO2001092291A3 PCT/IL2001/000496 IL0100496W WO0192291A3 WO 2001092291 A3 WO2001092291 A3 WO 2001092291A3 IL 0100496 W IL0100496 W IL 0100496W WO 0192291 A3 WO0192291 A3 WO 0192291A3
Authority
WO
WIPO (PCT)
Prior art keywords
antibody
cell receptor
cell
genes
receptor libraries
Prior art date
Application number
PCT/IL2001/000496
Other languages
French (fr)
Other versions
WO2001092291A2 (en
Inventor
Gideon Gross
Yaaqov Schlesinger
Original Assignee
Galim Galil Immunology Ltd
Gideon Gross
Yaaqov Schlesinger
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Galim Galil Immunology Ltd, Gideon Gross, Yaaqov Schlesinger filed Critical Galim Galil Immunology Ltd
Priority to AU62636/01A priority Critical patent/AU6263601A/en
Publication of WO2001092291A2 publication Critical patent/WO2001092291A2/en
Publication of WO2001092291A3 publication Critical patent/WO2001092291A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The invention relates to RNA trans-splicing-mediated covalent intracellular fusion of transcripts of two different genes A and B in a cell, particularly in an immune cell, in which said genes A and B are expressible, and to DNA constructs encoding RNAs useful in such processes. The genes A and B may encode the light and heavy chains of an antibody or the α and β chains of a T-cell receptor. This process leads to a unique and novel approach for creating immune antibody and T-cell receptor libraries.
PCT/IL2001/000496 2000-05-30 2001-05-30 Antibody and t-cell receptor libraries WO2001092291A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU62636/01A AU6263601A (en) 2000-05-30 2001-05-30 Antibody and t-cell receptor libraries

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IL136459 2000-05-30
IL13645900A IL136459A0 (en) 2000-05-30 2000-05-30 Antibody library

Publications (2)

Publication Number Publication Date
WO2001092291A2 WO2001092291A2 (en) 2001-12-06
WO2001092291A3 true WO2001092291A3 (en) 2002-05-16

Family

ID=11074201

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IL2001/000496 WO2001092291A2 (en) 2000-05-30 2001-05-30 Antibody and t-cell receptor libraries

Country Status (3)

Country Link
AU (1) AU6263601A (en)
IL (1) IL136459A0 (en)
WO (1) WO2001092291A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9181527B2 (en) 2009-10-29 2015-11-10 The Trustees Of Dartmouth College T cell receptor-deficient T cell compositions

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2002359913A1 (en) * 2001-12-20 2003-07-09 Agricultural Research Council Semi-synthetic antibody fragment combinatorial library based on avian immunoglobulin genes
PT1558643E (en) * 2002-11-09 2009-08-24 Immunocore Ltd T cell receptor display
TWI333977B (en) 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
GB0411771D0 (en) * 2004-05-26 2004-06-30 Avidex Ltd Nucleoproteins displaying native T cell receptor libraries
WO2006036445A2 (en) 2004-09-24 2006-04-06 Trustees Of Dartmouth College Chimeric nk receptor and methods for treating cancer
EP1831365B1 (en) * 2004-10-08 2013-08-07 VIRxSYS Corporation Use of rna trans-splicing for antibody gene transfer and antibody polypeptide production
AU2005295033B2 (en) * 2004-10-08 2012-01-19 Virxsys Corporation Targeted trans-splicing of highly abundant transcripts for in vivo production of recombinant proteins
WO2007131774A1 (en) 2006-05-17 2007-11-22 F. Hoffmann-La Roche Ag Polypeptide producing cells
JP5669397B2 (en) 2007-03-01 2015-02-12 シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ Methods for cloning cognate antibodies
DK2435568T3 (en) 2009-05-29 2014-09-08 Morphosys Ag Collection of synthetic antibodies to treat disease
US9273283B2 (en) 2009-10-29 2016-03-01 The Trustees Of Dartmouth College Method of producing T cell receptor-deficient T cells expressing a chimeric receptor
DK2528944T3 (en) 2010-01-29 2015-09-14 Morphosys Ag Rodent combinatorial antibody libraries
SG10201509257VA (en) 2010-11-19 2015-12-30 Morphosys Ag A collection and methods for its use
US9833476B2 (en) 2011-08-31 2017-12-05 The Trustees Of Dartmouth College NKP30 receptor targeted therapeutics
CN104395344B (en) 2012-05-07 2019-08-13 达特茅斯大学理事会 Anti- B7-H6 antibody, fusion protein and its application method
EP3164492B1 (en) * 2014-07-03 2019-10-23 F. Hoffmann-La Roche AG Polypeptide expression systems
US11292838B2 (en) 2015-06-01 2022-04-05 Medigene Immunotherapies Gmbh Method for generating antibodies against T cell receptor
NZ737400A (en) 2015-06-01 2019-09-27 Medigene Immunotherapies Gmbh T cell receptor library
MX2018007841A (en) 2015-12-23 2018-11-09 Medigene Immunotherapies Gmbh Dendritic cell composition.
WO2018060239A1 (en) 2016-09-29 2018-04-05 F. Hoffmann-La Roche Ag Improved method for selecting polypeptide producing cells

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5874299A (en) * 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies
US6162963A (en) * 1990-01-12 2000-12-19 Abgenix, Inc. Generation of Xenogenetic antibodies

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6162963A (en) * 1990-01-12 2000-12-19 Abgenix, Inc. Generation of Xenogenetic antibodies
US5874299A (en) * 1990-08-29 1999-02-23 Genpharm International, Inc. Transgenic non-human animals capable of producing heterologous antibodies

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HE M. ET AL.: "Selection of a human anti-progesterone antibody fragment from a transgenic mouse library by ARM ribosome display", J. IMMUNOL. METH., vol. 231, no. 1-2, 1999, pages 105 - 117, XP004187638 *
LORIMER I.A.J. ET AL.: "Recombinant immunotoxins specific for a mutant epidermal growth factor receptor: targeting with a single chain antibody variable domain isolated by phage display", PROC. NATL. ACAD. SCI. USA, vol. 93, no. 93, December 1996 (1996-12-01), pages 14815 - 14820, XP002912821 *
MCCALL A.M.: "Characterization of anti-mouse FcgammaRII single-chain Fv fragments derived fromhuman phage display libraries", IMMUNOTECHNOLOGY, vol. 4, 1998, pages 71 - 87, XP002908156 *
MEULEMANS E.V.: "Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody", J. MOL. BIOL., vol. 244, 1994, pages 353 - 360, XP002908155 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9181527B2 (en) 2009-10-29 2015-11-10 The Trustees Of Dartmouth College T cell receptor-deficient T cell compositions

Also Published As

Publication number Publication date
WO2001092291A2 (en) 2001-12-06
AU6263601A (en) 2001-12-11
IL136459A0 (en) 2001-06-14

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