WO2001050127A3 - Screening using biological target molecules with metal-ion binding sites - Google Patents

Screening using biological target molecules with metal-ion binding sites Download PDF

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Publication number
WO2001050127A3
WO2001050127A3 PCT/EP2000/013389 EP0013389W WO0150127A3 WO 2001050127 A3 WO2001050127 A3 WO 2001050127A3 EP 0013389 W EP0013389 W EP 0013389W WO 0150127 A3 WO0150127 A3 WO 0150127A3
Authority
WO
WIPO (PCT)
Prior art keywords
biological target
metal ion
ion binding
compounds
binding site
Prior art date
Application number
PCT/EP2000/013389
Other languages
French (fr)
Other versions
WO2001050127A2 (en
WO2001050127A8 (en
WO2001050127A9 (en
Inventor
Christian E Elling
Lars Ole Gerlach
Lange Birgitte Holst
Jan Torleif Pedersen
Thue W Schwartz
Original Assignee
7Tm Pharma
Christian E Elling
Lars Ole Gerlach
Lange Birgitte Holst
Jan Torleif Pedersen
Thue W Schwartz
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 7Tm Pharma, Christian E Elling, Lars Ole Gerlach, Lange Birgitte Holst, Jan Torleif Pedersen, Thue W Schwartz filed Critical 7Tm Pharma
Priority to EP00993741A priority Critical patent/EP1242824A2/en
Priority to AU28449/01A priority patent/AU2844901A/en
Priority to CA002395999A priority patent/CA2395999A1/en
Publication of WO2001050127A2 publication Critical patent/WO2001050127A2/en
Priority to PCT/DK2001/000867 priority patent/WO2002054077A2/en
Priority to AU2002215888A priority patent/AU2002215888A1/en
Publication of WO2001050127A3 publication Critical patent/WO2001050127A3/en
Publication of WO2001050127A9 publication Critical patent/WO2001050127A9/en
Publication of WO2001050127A8 publication Critical patent/WO2001050127A8/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention provides a molecular approach for rapidly and selectively identifying small organic molecule ligands, i.e. compounds, that are capable of interacting with and binding to specific sites on biological target molecules. The methods of the present invention are applicable to any biological target molecule that has or can be manipulated to have a metal ion binding site. A very interesting group of molecules are membrane proteins such as 7TM transmembrane proteins. The methods described herein make it possible to construct and screen libraries of compounds specifically directed against predetermined epitopes on the biological target molecules. The compounds are initially constructed to be bi-functional, i.e. having both a metal ion binding moiety enabling them to bind to a natural or artificial metal ion binding site, and a variable moiety, which is varied chemically to probe for interactions with specific parts of the biological target adjacent to the metal ion binding site. Compounds may subsequently be further modified to bind to the unmodified biological target molecule without help of the bridging metal ion.
PCT/EP2000/013389 1999-12-30 2000-12-29 Screening using biological target molecules with metal-ion binding sites WO2001050127A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
EP00993741A EP1242824A2 (en) 1999-12-30 2000-12-29 Screening using biological target molecules with metal-ion binding sites
AU28449/01A AU2844901A (en) 1999-12-30 2000-12-29 A method of identifying ligands of biological target molecules
CA002395999A CA2395999A1 (en) 1999-12-30 2000-12-29 Screening using biological target molecules with metal-ion binding sites
PCT/DK2001/000867 WO2002054077A2 (en) 2000-12-29 2001-12-21 Validating biological molecules as drug targets by metal-ion chelates in animal test models
AU2002215888A AU2002215888A1 (en) 2000-12-29 2001-12-21 Validating biological molecules as drug targets by metal-ion chelates in animal test models

Applications Claiming Priority (12)

Application Number Priority Date Filing Date Title
DK199901880 1999-12-30
DKPA199901880 1999-12-30
DK199901879 1999-12-30
DKPA199901879 1999-12-30
US17540100P 2000-01-11 2000-01-11
US17599400P 2000-01-11 2000-01-11
US60/175,994 2000-01-11
US60/175,401 2000-01-11
DK200000705 2000-04-28
DKPA200000705 2000-04-28
US20299000P 2000-05-09 2000-05-09
US60/202,990 2000-05-09

Publications (4)

Publication Number Publication Date
WO2001050127A2 WO2001050127A2 (en) 2001-07-12
WO2001050127A3 true WO2001050127A3 (en) 2002-01-31
WO2001050127A9 WO2001050127A9 (en) 2002-09-12
WO2001050127A8 WO2001050127A8 (en) 2004-02-19

Family

ID=27545201

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/013389 WO2001050127A2 (en) 1999-12-30 2000-12-29 Screening using biological target molecules with metal-ion binding sites

Country Status (4)

Country Link
EP (1) EP1242824A2 (en)
AU (1) AU2844901A (en)
CA (1) CA2395999A1 (en)
WO (1) WO2001050127A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8975082B2 (en) 2008-05-13 2015-03-10 University Of Kansas Metal abstraction peptide (MAP) tag and associated methods
US9187735B2 (en) 2012-06-01 2015-11-17 University Of Kansas Metal abstraction peptide with superoxide dismutase activity

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003003008A1 (en) * 2001-06-29 2003-01-09 7Tm Pharma A/S Chemical libraries useful for drug discovery processes
WO2003003009A1 (en) * 2001-06-29 2003-01-09 7Tm Pharma A/S Use of metal-ion chelates in validating biological molecules as drug targets in test animal models
AU2002358469A1 (en) * 2001-12-21 2003-07-15 7Tm Pharma A/S Modified receptors for the discovery of therapeutic ligands
AU2002367087A1 (en) * 2001-12-21 2003-07-15 7Tm Pharma A/S Method for the treatment of mc receptor related disorders with a chelate and/or a chelator
CN110390997B (en) * 2019-07-17 2023-05-30 成都火石创造科技有限公司 Chemical molecular formula splicing method
WO2021263060A2 (en) * 2020-06-24 2021-12-30 Lycia Therapeutics, Inc. Bifunctional bridging compositions for viral transduction

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996041169A1 (en) * 1995-06-07 1996-12-19 Praecis Pharmaceuticals Incorporated Functional bioassay for g-protein coupled receptor agonists and antagonists
WO1996040293A1 (en) * 1995-06-07 1996-12-19 Rhomed Incorporated Structurally determined metallo-constructs and applications
WO1998012156A1 (en) * 1996-09-20 1998-03-26 President And Fellows Of Harvard College Combinatorial approach for generating novel coordination complexes
WO1999022733A1 (en) * 1997-10-31 1999-05-14 Smithkline Beecham Corporation Novel metal complexes
WO2000051632A2 (en) * 1999-03-03 2000-09-08 Konrad Beyreuther COPPER AGONIST THAT BINDS ON THE COPPER BINDING SITE OF APP AND/OR EXERTS AN INHIBITING EFFECT ON THE RELEASE OF AMYLOID Aβ PEPTIDE
WO2001013112A1 (en) * 1999-08-12 2001-02-22 Palatin Technologies, Inc. Melanocortin metallopeptide constructs, combinatorial libraries and applications

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996041169A1 (en) * 1995-06-07 1996-12-19 Praecis Pharmaceuticals Incorporated Functional bioassay for g-protein coupled receptor agonists and antagonists
WO1996040293A1 (en) * 1995-06-07 1996-12-19 Rhomed Incorporated Structurally determined metallo-constructs and applications
WO1998012156A1 (en) * 1996-09-20 1998-03-26 President And Fellows Of Harvard College Combinatorial approach for generating novel coordination complexes
WO1999022733A1 (en) * 1997-10-31 1999-05-14 Smithkline Beecham Corporation Novel metal complexes
WO2000051632A2 (en) * 1999-03-03 2000-09-08 Konrad Beyreuther COPPER AGONIST THAT BINDS ON THE COPPER BINDING SITE OF APP AND/OR EXERTS AN INHIBITING EFFECT ON THE RELEASE OF AMYLOID Aβ PEPTIDE
WO2001013112A1 (en) * 1999-08-12 2001-02-22 Palatin Technologies, Inc. Melanocortin metallopeptide constructs, combinatorial libraries and applications

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ELLING CHRISTIAN E ET AL: "Connectivity and orientation of the seven helical bundle in the tachykinin NK-1 receptor probed by zinc site engineering.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 15, no. 22, 1996, pages 6213 - 6219, XP002174723, ISSN: 0261-4189 *
ELLING CHRISTIAN E ET AL: "Conversion of agonist site to metal-ion chelator site in the beta2-adrenergic receptor.", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES, vol. 96, no. 22, 26 October 1999 (1999-10-26), Oct. 26, 1999, pages 12322 - 12327, XP002174721, ISSN: 0027-8424 *
ELLING CHRISTIAN E ET AL: "Disulfide bridge engineering in the tachykinin NK1 receptor.", BIOCHEMISTRY, vol. 39, no. 4, 1 February 2000 (2000-02-01), pages 667 - 675, XP002174724, ISSN: 0006-2960 *
NORREGAARD LENE ET AL: "Delineation of an endogenous zinc-binding site in the human dopamine transporter.", EMBO (EUROPEAN MOLECULAR BIOLOGY ORGANIZATION) JOURNAL, vol. 17, no. 15, 3 August 1998 (1998-08-03), pages 4266 - 4273, XP002174722, ISSN: 0261-4189 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8975082B2 (en) 2008-05-13 2015-03-10 University Of Kansas Metal abstraction peptide (MAP) tag and associated methods
US9096652B2 (en) 2008-05-13 2015-08-04 University Of Kansas Metal abstraction peptide (MAP) tag and associated methods
US9187735B2 (en) 2012-06-01 2015-11-17 University Of Kansas Metal abstraction peptide with superoxide dismutase activity

Also Published As

Publication number Publication date
AU2844901A (en) 2001-07-16
WO2001050127A2 (en) 2001-07-12
CA2395999A1 (en) 2001-07-12
WO2001050127A8 (en) 2004-02-19
WO2001050127A9 (en) 2002-09-12
EP1242824A2 (en) 2002-09-25

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