WO2001049740A1 - Novel fgf homolog zfgf12 - Google Patents
Novel fgf homolog zfgf12 Download PDFInfo
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- WO2001049740A1 WO2001049740A1 PCT/US2001/000238 US0100238W WO0149740A1 WO 2001049740 A1 WO2001049740 A1 WO 2001049740A1 US 0100238 W US0100238 W US 0100238W WO 0149740 A1 WO0149740 A1 WO 0149740A1
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- zfgf12
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
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Definitions
- the fibroblast growth factor (FGF) family consists of at least eighteen distinct members (Basilico et al., Adv. Cancer Res. 59:1 15-165, 1992 and Fernig et al., Prog. Growth Factor Res. 5(4):353-377, 1994) which generally act as mitogens for a broad spectrum of cell types.
- basic FGF also known as FGF-2
- FGF-2 is mitogenic in vitro for endothelial cells, vascular smooth muscle cells, fibroblasts, and generally for cells of mesoderm or neuroectoderm origin, including cardiac and skeletal myocytes (Gospodarowicz et al., J. Cell. Biol.
- Non- proliferative activities associated with acidic and/or basic FGF include: increased endothelial release of tissue plasminogen activator, stimulation of extracellular matrix synthesis, chemotaxis for endothelial cells, induced expression of fetal contractile genes in cardiomyocytes (Parker et al., J. Clin. Invest. 85:507-514, 1990), and enhanced pituitary hormonal responsiveness (Baird et al., J. Cellular Physiol. 5: 101 -106, 1987.)
- FGF receptors There are four known extracellular FGF receptors (FGFRs), and they are all tyrosine kinases. In general, the FGF family members bind to all of the known FGFRs, however, specific FGFs bind to specific receptors with higher degrees of affinity. Another means for specificity within the FGF family is the spatial and temporal expression of the ligands and their receptors during embryogenesis. Evidence suggests that the FGFs most likely act only in autocrine and/or paracrine manner, due to their heparin binding affinity, which limits their diffusion from the site of release (Flaumenhaft et al., J. Cell. Biol.
- Basic FGF lacks a signal sequence, and is therefore restricted to paracrine or autocrine modes of action. It has been postulated that basic FGF is stored intracellularly and released upon tissue damage. Basic FGF has been shown to have two receptor binding regions that are distinct from the heparin binding site (Abraham et al.,. EMBO J. 5(10):2523-2528, 1986.)
- FGF family Members of the FGF family have been shown to play important roles developmentally and in adult tissue. The activities of the family members appear to be promiscuous in some tissues and have tissue-specificity in other cases.
- the present invention provides a novel member of the FGF family and the uses for these polynucleotides and polypeptides should be apparent to those skilled in the art from the teachingt s herein.
- affinity tag is used herein to denote a polypeptide segment that can be attached to a second polypeptide to provide for purification or detection of the second polypeptide or provide sites for attachment of the second polypeptide to a substrate.
- Affinity tags include a poly- histidine tract, protein A (Nilsson et al., EMBO J. 4: 1075, 1985; Nilsson et al., Methods Enzvmol. 198:3, 1991), glutathione S transferase (Smith and Johnson, Gene 67:31, 1988), Glu-Glu affinity tag (Grussenmeyer et al., Proc.
- allelic variant is used herein to denote any of two or more alternative forms of a gene occupying the same chromosomal locus. Allelic variation arises naturally through mutation, and may result in phenotypic polymo ⁇ hism within populations. Gene mutations can be silent (no change in the encoded polypeptide) or may encode polypeptides having altered amino acid sequence.
- allelic variant is also used herein to denote a protein encoded by an allelic variant of a gene.
- amino-terminal and “carboxyl-terminal” are used herein to denote positions within polypeptides. Where the context allows, these terms are used with reference to a particular sequence or portion of a polypeptide to denote proximity or relative position. For example, a certain sequence positioned carboxyl-terminal to a reference sequence within a polypeptide is located proximal to the carboxyl terminus of the reference sequence, but is not necessarily at the carboxyl terminus of the complete polypeptide.
- complement/anti-complement pair denotes non-identical moieties that form a non-covalently associated, stable pair under appropriate conditions.
- biotin and avidin are prototypical members of a complement/anti-complement pair.
- Other exemplary complement/anti-complement pairs include receptor/ligand pairs, antibody/antigen (or hapten or epitope) pairs, sense/antisense polynucleotide pairs, and the like. Where subsequent dissociation of the complement/anti-complement pair is desirable, the complement/anti-complement pair preferably has a binding affinity of ⁇ 10° M ⁇ l.
- polynucleotide molecule is a polynucleotide molecule having a complementary base sequence and reverse orientation as compared to a reference sequence.
- sequence 5' ATGCACGGG 3' is complementary to 5' CCCGTGCAT 3'.
- degenerate nucleotide sequence denotes a sequence of nucleotides that includes one or more degenerate codons (as compared to a reference polynucleotide molecule that encodes a polypeptide). Degenerate codons contain different triplets of nucleotides, but encode the same amino acid residue (i.e., GAU and
- expression vector is used to denote a DNA molecule, linear or circular, that comprises a segment encoding a polypeptide of interest operably linked to additional segments that provide for its transcription.
- additional segments include promoter and terminator sequences, and may also include one or more origins of replication, one or more selectable markers, an enhancer, a polyadenylation signal, etc.
- Expression vectors are generally derived from plasmid or viral DNA, or may contain elements of both.
- isolated when applied to a polynucleotide, denotes that the polynucleotide has been removed from its natural genetic milieu and is thus free of other extraneous or unwanted coding sequences, and is in a form suitable for use within genetically engineered protein production systems.
- isolated molecules are those that are separated from their natural environment and include cDNA and genomic clones.
- Isolated DNA molecules of the present invention are free of other genes with which they are ordinarily associated, but may include naturally occurring 5' and 3' untranslated regions such as promoters and terminators. The identification of associated regions will be evident to one of ordinary skill in the art (see for example, Dynan and Tijan, Nature 316:774-78, 1985).
- an "isolated" polypeptide or protein is a polypeptide or protein that is found in a condition other than its native environment, such as apart from blood and animal tissue.
- the isolated polypeptide is substantially free of other polypeptides, particularly other polypeptides of animal origin. It is preferred to provide the polypeptides in a highly purified form, i.e. greater than 95% pure, more preferably greater than 99% pure.
- the term “isolated” does not exclude the presence of the same polypeptide in alternative physical forms, such as di ers or alternatively glycosylated or derivatized forms.
- the term "operably linked" when referring to DNA segments, indicates that the segments are arranged so that they function in concert for their intended pu ⁇ oses, e.g., transcription initiates in the promoter and proceeds through the coding segment to the terminator.
- ortholog denotes a polypeptide or protein obtained from one species that is the functional counte ⁇ art of a polypeptide or protein from a different species. Sequence differences among orthologs are the result of speciation.
- ortholog denotes a polypeptide or protein obtained from one species that is the functional counte ⁇ art of a polypeptide or protein from a different species. Sequence differences among orthologs are the result of speciation.
- a "polynucleotide” is a single- or double-stranded polymer of deoxyribonucleotide or ribonucleotide bases read from the 5' to the 3' end. Polynucleotides include RNA and DNA, and may be isolated from natural sources, synthesized in vitro, or prepared from a combination of natural and synthetic molecules.
- bp base pairs
- nt nucleotides
- kb kilobases
- polypeptide ' is a polymer of amino acid residues joined by peptide bonds, whether produced naturally or synthetically. Polypeptides of less than about 10 amino acid residues are commonly referred to as "peptides".
- promoter is used herein for its art-recognized meaning to denote a portion of a gene containing DNA sequences that provide for the binding of RNA polymerase and initiation of transcription. Promoter sequences are commonly, but not always, found in the 5' non-coding regions of genes.
- a “protein” is a macromolecule comprising one or more polypeptide chains.
- a protein may also comprise non-peptidic components, such as carbohydrate groups. Carbohydrates and other non-peptidic substituents may be added to a protein by the cell in which the protein is produced, and will vary with the type of cell. Proteins are defined herein in terms of their amino acid backbone structures; substituents such as carbohydrate groups are generally not specified, but may be present nonetheless.
- the term "receptor” denotes a cell-associated protein that binds to a bioactive molecule (i.e., a ligand) and mediates the effect of the ligand on the cell.
- Membrane-bound receptors are characterized by a multi-peptide structure comprising an extracellular ligand-binding domain and an intracellular effector domain that is typically involved in signal transduction. Binding of ligand to receptor results in a conformational change in the receptor that causes an interaction between the effector domain and other molecule(s) in the cell. This interaction in turn leads to an alteration in the metabolism of the cell. Metabolic events that are linked to receptor-ligand interactions include gene transcription, phosphorylation, dephosphorylation, increases in cyclic AMP production, mobilization of cellular calcium, mobilization of membrane lipids, cell adhesion, hydrolysis of inositol lipids and hydrolysis of phospholipids.
- receptors can be membrane bound, cytosolic or nuclear; monomeric (e.g., thyroid stimulating hormone receptor, beta-adrenergic receptor) or multimeric (e.g., PDGF receptor, growth hormone receptor, IL-3 receptor, GM-CSF receptor, G-CSF receptor, erythropoietin receptor and IL-6 receptor).
- secretory signal sequence denotes a DNA sequence that encodes a polypeptide (a "secretory peptide”) that, as a component of a larger polypeptide, directs the larger polypeptide through a secretory pathway of a cell in which it is synthesized. The larger polypeptide is commonly cleaved to remove the secretory peptide during transit through the secretory pathway.
- splice variant is used herein to denote alternative forms of
- RNA transcribed from a gene Splice variation arises naturally through use of alternative splicing sites within a transcribed RNA molecule, or less commonly between separately transcribed RNA molecules, and may result in several mRNAs transcribed from the same gene Splice variants may encode polypeptides having altered amino acid sequence.
- the term splice variant is also used herein to denote a protein encoded by a splice variant of an mRNA transcribed from a gene
- the present invention is based in part upon the discovery of a novel DNA sequence that encodes a fibroblast growth factor (FGF) homolog polypeptide having approximately 31% homology to FGF- 19 (Nishimura et al., Biochem Biophy. Acta 1444: 148-151, 1999)
- FGF fibroblast growth factor
- the novel zFGF12 polypeptides of the present invention contain a motif known to occur in all known members of the FGF family, and is unique to these proteins
- the zFGF12 homolog polypeptide encoded by DNA contains a variation of the motif formula: CXFXE, wherein X is any amino acid and X ⁇ ⁇ is the number of X amino acids greater than one (SEQ ID NO: 5)
- This motif is highly conserved in all members of the FGF family, however, zFGF12 appears to be unique in that the conserved Glu is a His (residue 1 17) substituting a basic residue for an acidic residue
- a consensus amino acid sequence of the domain includes, for example, human myocyte-activating factor (FGF- 10; HSU76381, GENBANK identifier, NCBI), human fibroblast growth factor homologous factor 4 (FHF-4; Smallwood et al., 1996, ibid ), human fibroblast growth factor homologous factor 3 (FHF-3,
- human FGF- 6 Basilico et al., 1992, ibid
- human FGF-2 basic; Basilico et al., 1992, ibid.
- human FGF-1 acidic; Basilico et al., 1992, ibid.
- human keratinocyte growth factor precursor FGF-7; Basilico et al., 1992, ibid
- human FGF-5 Basilico et al., 1992, ibid.
- human FGF-9 Miyamoto et al., Mol. Cell Biol.
- SEQ ID NO: 2 forms an open reading frame encoding 251 amino acids (SEQ ID NO: 2) comprising a mature polypeptide of 227 amino acids (residue 25 to residue 251 of SEQ ID NO: 2) with a secretory signal sequence of 24 amino acids (residue 1 to 24 of SEQ ID NO: 2).
- Multiple alignment of zFGF12 with other known FGFs revealed a block of high percent identity corresponding to amino acid residue 82 to 131 of SEQ ID NO: 2.
- the FGF family motif as shown in SEQ ID NO: 5, corresponds to amino acid residues 1 13 (Cys) to 1 17 (His) of SEQ ID NO: 2.
- Several of the members of the FGF family do not have signal sequences.
- beta strand structure of zFGF12 includes the following regions of amino acid residues: strand 2 — 51 (Tyr)-56 (Lys); strand 3—59 (Gly)-64 (Ala); strand 4—71 (Ser)-77 (Ser); strand 5— 81 (Gly)-88 (Val); strand 6—92 (Arg)-98 (Phe); strand 7—99 (Arg)- 105 (Ser); strand 8—1 13 (Cys)-120 (Leu); strand 9—123 (Gly)-130 (Phe); strand 10—134 (Phe)-140 (Arg); and strand 11-143 (Arg)-147 (Pro), as shown in SEQ ID NO: 2.
- Amino acids critical for zFGF12 binding to receptors can be identified by site-directed mutagenesis of the entire zFGF12 polypeptide. More specifically, they can be identified using site- directed mutagenesis of amino acids in the zFGF12 polypeptide which correspond to amino acid residues in acidic FGF (FGF1) and basic FGF (FGF2) which have been identified as critical for binding to their respective receptors (Blaber et al., Biochem. 35:2086-2094, 1996). In zFGF12 hydrophobic residues buried within the core of the protein will be relatively intolerant of substitution, particularly polar or charged residues.
- Residues critical to the beta-trefoil fold of the zFGF12 include residues 53 (Leu), 61 (Val), 73 (Leu), 75 (He), 83 (Val), 85 (He), 94 (Val), 102 (Leu), 1 15 (Phe), 127 (Tyr), and 136 (Val).
- FGF family may have structural or biochemical similarities to zFGF12. Therefore, amino acid residues from another FGF family member can be used for substitutions at corresponding positions in zFGF12 given the limitations disclosed herein. Those skilled in the art will recognize that predicted domain boundaries are somewhat imprecise and may vary by up to ⁇ 3 amino acid residues.
- Polypeptides of the present invention comprise at least 6, at least 9, or at least 15 contiguous amino acid residues of SEQ ID NO:2.
- the polypeptides comprise 20, 30, 40, 50, 100, or more contiguous residues of SEQ ID NO:2, up to the entire predicted mature polypeptide (residues 25 to 251 of SEQ ID NO:2) or the primary translation product (residues 1 to 251 of SEQ ID NO:2).
- these polypeptides can further comprise additional, non-zFGF12, polypeptide sequence(s).
- epitopes are regions of a protein to which an antibody can bind. See, for example, Geysen et al., Proc. Natl. Acad. Sci. USA 81:3998-4002, 1984. Epitopes can be linear or conformational, the latter being composed of discontinuous regions of the protein that form an epitope upon folding of the protein. Linear epitopes are generally at least 6 amino acid residues in length. Relatively short synthetic peptides that mimic part of a protein sequence are routinely capable of eliciting an antiserum that reacts with the partially mimicked protein. See, Sutcliffe et al., Science 219:660-666, 1983.
- Antibodies that recognize short, linear epitopes are particularly useful in analytic and diagnostic applications that employ denatured protein, such as Western blotting (Tobin, Proc. Natl. Acad. Sci. USA 76:4350-4356, 1979), or in the analysis of fixed cells or tissue samples.
- Antibodies to linear epitopes are also useful for detecting fragments of zFGF12, such as might occur in body fluids or cell culture media.
- Antigenic, epitope-bearing polypeptides of the present invention are useful for raising antibodies, including monoclonal antibodies, that specifically bind to a zFGF12 protein.
- Antigenic, epitope-bearing polypeptides contain a sequence of at least six, preferably at least nine, more preferably from 15 to about 30 contiguous amino acid residues of a zFGF12 protein (e.g., SEQ ID NO:2). Polypeptides comprising a larger portion of a zFGF12 protein, i.e. from 30 to 50 residues up to the entire sequence, are included. It is preferred that the amino acid sequence of the epitope-bearing polypeptide is selected to provide substantial solubility in aqueous solvents, that is the sequence includes relatively hydrophilic residues, and hydrophobic residues are substantially avoided. Specific, useful polypeptides in this regard include those comprising residues 182-187, 179-184, 175-180, 174-179, and 76-81 of SEQ ID NO:2
- Polypeptides of the present invention can be prepared with one or more amino acid substitutions, deletions or additions as compared to SEQ ID NO:2. These changes are preferably of a minor nature, that is conservative amino acid substitutions and other changes that do not significantly affect the folding or activity of the protein or polypeptide as described herein. These changes include amino- or carboxyl-terminal extensions, such as an amino-terminal methionine residue, an amino or carboxyl- terminal cysteine residue to facilitate subsequent linking to maleimide-activated keyhole limpet hemocyanin, a small linker peptide of up to about 20-25 residues, or an extension that facilitates purification (an affinity tag) as disclosed above. Two or more affinity tags may be used in combination.
- Polypeptides comprising affinity tags can further comprise a polypeptide linker and/or a proteolytic cleavage site between the zFGF12 polypeptide and the affinity tag.
- Preferred cleavage sites include thrombin cleavage sites and factor Xa cleavage sites.
- the present invention further provides a variety of other polypeptide fusions.
- a zFGF12 polypeptide can be prepared as a fusion to a dimerizing protein as disclosed in U.S. Patents Nos. 5,155,027 and 5,567,584.
- Preferred dimerizing proteins in this regard include immunoglobulin constant region domains.
- Immunoglobulin-zFGF12 polypeptide fusions can be expressed in genetically engineered cells to produce a variety of multimeric zFGF12 analogs.
- a zFGF12 polypeptide can be joined to another bioactive molecule, such as a cytokine, to provide a multi-functional molecule.
- One or more helices of a zFGF12 polypeptide can be joined to another cytokine to enhance or otherwise modify its biological properties.
- Auxiliary domains can be fused to zFGF12 polypeptides to target them to specific cells, tissues, or macromolecules (e.g., collagen).
- a zFGF12 polypeptide or protein can be targeted to a predetermined cell type by fusing a zFGF12 polypeptide to a ligand that specifically binds to a receptor on the surface of the target cell. In this way, polypeptides and proteins can be targeted for therapeutic or diagnostic pu ⁇ oses.
- a zFGF12 polypeptide can be fused to two or more moieties, such as an affinity tag for purification and a targeting domain.
- Polypeptide fusions can also comprise one or more cleavage sites, particularly between domains. See, Tuan et al., Connective Tissue Research 34: 1-9, 1996.
- Polypeptide fusions of the present invention will generally contain not more than about 1,500 amino acid residues, preferably not more than about 1,200 residues, more preferably not more than about 1 ,000 residues, and will in many cases be considerably smaller.
- a zFGF12 polypeptide of 227 residues can be fused to E. coli ?-galactosidase (1,021 residues; see Casadaban et al., J. Bacteriol. 143:971-980, 1980), a 10-residue spacer, and a 4-residue factor Xa cleavage site to yield a polypeptide of 1262 residues.
- residues 25-251 SEQ ID NO:2 can be fused to maltose binding protein (approximately 370 residues), a 4-residue cleavage site, and a 6-residue polyhistidine tag.
- the polypeptides of the present invention comprise at least 6 contiguous residues of SEQ ID NO:2. These polypeptides may further comprise additional residues as shown in SEQ ID NO:2, a variant of SEQ ID NO:2, or another protein as disclosed herein.
- the resulting polypeptide will be at least 80% to 90% or in other embodiments, at least 95%, 96%, 97%, 98%, or 99% identical to the corresponding region of SEQ ID NO:2. Percent sequence identity is determined by conventional methods. See, for example, Altschul et al., Bull. Math. Bio. 48:603-616, 1986, and Henikoff and Henikoff, Proc.
- the ten regions with the highest density of identities are then rescored by comparing the similarity of all paired amino acids using an amino acid substitution matrix, and the ends of the regions are "trimmed" to include only those residues that contribute to the highest score. If there are several regions with scores greater than the "cutoff value (calculated by a predetermined formula based upon the length of the sequence and the ktup value), then the trimmed initial regions are examined to determine whether the regions can be joined to form an approximate alignment with gaps. Finally, the highest scoring regions of the two amino acid sequences are aligned using a modification of the Needleman-Wunsch-Sellers algorithm (Needleman and Wunsch, J. Mol. Biol. 48:444, 1970; Sellers, SIAM J. Appl. Math.
- FASTA can also be used to determine the sequence identity of nucleic acid molecules using a ratio as disclosed above.
- the ktup value can range between one to six, preferably from three to six, most preferably three, with other parameters set as default.
- the present invention includes polypeptides having one or more conservative amino acid changes as compared with the amino acid sequence of SEQ ID NO:2.
- the BLOSUM62 matrix (Table 1) is an amino acid substitution matrix derived from about 2,000 local multiple alignments of protein sequence segments, representing highly conserved regions of more than 500 groups of related proteins (Henikoff and
- the BLOSUM62 substitution frequencies can be used to define conservative amino acid substitutions that may be introduced into the amino acid sequences of the present invention.
- conservative amino acid substitution refers to a substitution represented by a BLOSUM62 value of greater than -1.
- an amino acid substitution is conservative if the substitution is characterized by a BLOSUM62 value of 0, 1, 2, or 3.
- Preferred conservative amino acid substitutions are charac erized by a BLOSUM62 value of at least one 1 (e.g., 1 , 2 or 3), while more preferred conservative amino acid substitutions are characterized by a BLOSUM62 value of at least 2 (e.g., 2 or 3).
- the proteins of the present invention can also comprise non-naturally occuring amino acid residues.
- Non-naturally occuring amino acids include, without limitation, trans-3-methylproline, 2,4-methanoproline, c/.v-4-hydroxyproline, trans-4- hydroxyproline, /V-methylglycine, //o-threonine, methylthreonine, hydroxyethylcysteine, hydroxyethylhomocysteine, nitroglutamine, homoglutamine, pipecolic acid, tert-leucine, norvaline, 2-azaphenylalanine, 3-azaphenylalanine, 4- azaphenylalanine, and 4-fluorophenylalanine.
- coli cells are cultured in the absence of a natural amino acid that is to be replaced (e.g., phenylalanine) and in the presence of the desired non-naturally occurring amino acid(s) (e.g., 2-azaphenylalanine, 3-azaphenylalanine, 4-azaphenylalanine, or 4- fluorophenylalanine).
- the non-naturally occurring amino acid is inco ⁇ orated into the protein in place of its natural counte ⁇ art. See, Koide et al., Biochem. 33:7470-7476, 1994.
- Naturally occurring amino acid residues can be converted to non-naturally occurring species by in vitro chemical modification. Chemical modification can be combined with site-directed mutagenesis to further expand the range of substitutions
- Amino acid sequence changes are made in zFGF12 polypeptides so as to minimize disruption of higher order structure essential to biological activity as disclosed previously.
- Amino acid residues that are within regions or domains that are critical to maintaining structural integrity can be determined. Within these regions one can identify specific residues that will be more or less tolerant of change and maintain the overall tertiary structure of the molecule.
- Methods for analyzing sequence structure include, but are not limited to, alignment of multiple sequences with high amino acid or nucleotide identity, secondary structure propensities, binary patterns, complementary packing, and buried polar interactions (Barton, Current Opin. Struct. Biol. 5:372-376, 1995 and Cordes et al., Current Opin. Struct. Biol.
- Essential amino acids in the polypeptides of the present invention can be identified experimentally according to procedures known in the art, such as site- directed mutagenesis or alanine-scanning mutagenesis (Cunningham and Wells,
- Variants of the disclosed zFGF12 DNA and polypeptide sequences can be generated through DNA shuffling as disclosed by Stemmer, Nature 370:389-391, 1994 and Stemmer, Proc. Natl. Acad. Sci. USA 91 : 10747-10751 , 1994. Briefly, variant genes are generated by in vitro homologous recombination by random fragmentation of a parent gene followed by reassembly using PCR, resulting in randomly introduced point mutations. This technique can be modified by using a family of parent genes, such as allelic variants or genes from different species, to introduce additional variability into the process. Selection or screening for the desired activity, followed by additional iterations of mutagenesis and assay provides for rapid "evolution" of sequences by selecting for desirable mutations while simultaneously selecting against detrimental changes.
- the structure of the final polypeptide product will result from processing of the nascent polypeptide chain by the host cell, thus the final sequence of a zFGF12 polypeptide produced by a host cell will not always correspond to the full sequence encoded by the expressed polynucleotide.
- expressing the complete zFGF12 sequence in a cultured mammalian cell is expected to result in removal of at least the secretory peptide, while the same polypeptide produced in a prokaryotic host would not be expected to be cleaved.
- Differential processing of individual chains may result in heterogeneity of expressed polypeptides.
- SEQ ID NO: 3 is a degenerate polynucleotide sequence that encompasses all polynucleotides that could encode the zFGF12 polypeptide of SEQ ID NO: 2 (amino acids 1 or 25 to 251).
- zFGF12 polypeptide-encoding polynucleotides ranging from nucleotide 1 or 72 to nucleotide 753 of SEQ ID NO: 3 are contemplated by the present invention.
- degenerate codon representative of all possible codons encoding each amino acid.
- WSN can, in some circumstances, encode arginine
- MGN can, in some circumstances, encode serine
- some polynucleotides encompassed by the degenerate sequence may have some variant amino acids, but one of ordinary skill in the art can easily identify such variant sequences by reference to the amino acid sequence of SEQ ID NO: 2. Variant sequences can be readily tested for functionality as described herein.
- preferential codon usage or “preferential codons” is a term of art referring to protein translation codons that are most frequently used in cells of a certain species, thus favoring one or a few representatives of the possible codons encoding each amino acid (See Table 2).
- the amino acid Threonine (Thr) may be encoded by ACA, ACC, ACG, or ACT, but in mammalian cells ACC is the most commonly used codon; in other species, for example, insect cells, yeast, viruses or bacteria, different Thr codons may be preferential.
- Preferential codons for a particular species can be introduced into the polynucleotides of the present invention by a variety of methods known in the art.
- preferential codon sequences into recombinant DNA can, for example, enhance production of the protein by making protein translation more efficient within a particular cell type or species. Therefore, the degenerate codon sequence disclosed in SEQ ID NO: 3 serves as a template for optimizing expression of polynucleotides in various cell types and species commonly used in the art and disclosed herein. Sequences containing preferential codons can be tested and optimized for expression in various species, and tested for functionality as disclosed herein.
- the highly conserved amino acids in zFGF12 can be used as a tool to identify new family members.
- the conserved CXFXE motif SEQ ID NO: 5
- RNA obtained from a variety of tissue sources can be used to generate cDNA libraries and probe these libraries for new family members.
- reverse transcription-polymerase chain reaction RT- PCR
- RT- PCR reverse transcription-polymerase chain reaction
- the present invention provides methods for using zFGF12 polynucleotides and polypeptides to identify chromosomal disorders associated with abnormal expression of the zFGF12 protein.
- Detectable chromosomal mutations at the zFGF12 gene locus include, but are not limited to, aneuploidy, gene copy number changes, insertions, deletions, translocations, restriction site changes and rearrangements.
- Such aberrations can be identified by employing molecular genetic techniques, such as restriction fragment length polymo ⁇ hism (RFLP) analysis, short tandem repeat (STR) analysis employing PCR techniques, and other genetic linkage analysis techniques known in the art (Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989, and Ausubel et al., eds., Current Protocols in Molecular Biology. John Wiley and Sons, Inc., NY, 1987; A.J. Marian, Chest 108:255- 65, 1995). Analyses of DNA samples can detect deletions and insertions by changes in size in amplified DNA products by comparing a sample DNA to a normal zFGF12 DNA standard.
- RFLP restriction fragment length polymo ⁇ hism
- STR short tandem repeat
- Mismatches in duplex DNA can be detected by RNase digestion or differences in melting temperature.
- Other methods for detecting differences in sequences include changes in electrophoretic motility, Southern analysis, and direct DNA sequencing.
- Recently, techniques for accessing genetic information with high- density arrays have been available (Chee et al., Science 274:610-614, 1996), and can analyze large fragments of genomic DNA with high resolution. Analysis of chromosomal DNA using the zFGF12 polynucleotide sequence is useful for correlating disease with mutations localized to the chromosome where the zFGF12 gene resides.
- the methods of the present invention provide a method of detecting a zFGF12 chromosomal abnormality in sample from an individual comprising: (a) obtaining a zFGF12 RNA from the sample; (b) generating zFGF12 cDNA by polymerase chain reaction; and (c) comparing the nucleic acid sequence of the zFGF12 cDNA to the nucleic acid sequence as shown in SEQ ID NO: 1.
- the difference between the sequence of the zFGF12 cDNA or zFGF12 gene in the sample and the zFGF12 sequence as shown in SEQ ID NO: 1 is indicative of a zFGF12 chromosomal mutation.
- introns, splice acceptor or splice donor abnormalities can be detected by comparison of genomic sequences from a patient to a standard genomic sequence.
- kits for performing a diagnostic assay for ZFGF12 gene expression or to analyze the ZFGF12 locus of a subject comprise nucleic acid probes, such as double-stranded nucleic acid molecules comprising the nucleotide sequence of SEQ ID NOS.
- Probe molecules may be DNA, RNA, oligonucleotides, and the like Kits may comprise nucleic acid primers for performing PCR
- kits can contain all the necessary elements to perform a nucleic acid diagnostic assay described above
- a kit will comprise at least one container comprising a ZFGF12 probe or primer
- the kit may also comprise a second container comprising one or more reagents capable of indicating the presence of ZFGF12 sequences
- indicator reagents include detectable labels such as radioactive labels, fluorochromes, chemiluminescent agents, and the like
- a kit may also comprise a means for conveying to the user that the ZFGF12 probes and primers are used to detect ZFGF12 gene expression.
- written instructions may state that the enclosed nucleic acid molecules can be used to detect either a nucleic acid molecule that encodes ZFGF12, or a nucleic acid molecule having a nucleotide sequence that is complementary to a ZFGF12-encod ⁇ ng nucleotide sequence, or to analyze chromosomal sequences associated with the ZFGF12 locus
- the written material can be applied directly to a container, or the written material can be provided in the form of a packaging insert
- the isolated nucleic acid molecules can hybridize under stringent conditions to nucleic acid molecules having at least a portion of the nucleotide sequence of SEQ ID NOs 1 or 3 or to nucleic acid molecules having a nucleotide sequence complementary to those sequences
- a pair of nucleic acid molecules such as DNA-DNA, RNA-RNA and DNA-RNA, can hybridize if the nucleotide sequences have some degree of complementarity Hybrids can tolerate mismatched base pairs in the double helix, but the stability of the hybrid is influenced by the degree of mismatch.
- the T m of the mismatched hybrid decreases by 1 °C for every 1-1 5% base pair mismatch Varying the stringency of the hybridization conditions allows control over the degree of mismatch that will be present in the hybrid
- the degree of stringency increases as the hybridization temperature increases and the ionic strength of the hybridization buffer decreases.
- Stringent hybridization conditions encompass temperatures of about 5-25°C below the T m of the hybrid and a hybridization buffer having up to 1 M Na + Higher degrees of stringency at lower temperatures can be achieved with the addition of formamide which reduces the T m of the hybrid about 1 °C for eac h 1 % formamide in the buffer solution.
- such stringent conditions include temperatures of 20-70°C and a hybridization buffer containing up to 6xSSC and 0-50% formamide.
- a higher degree of stringency can be achieved at temperatures of from 40-70°C with a hybridization buffer having up to 4xSSC and from 0-50% formamide.
- Highly stringent conditions typically encompass temperatures of 42-70°C with a hybridization buffer having up to lxSSC and 0-50% formamide.
- Different degrees of stringency can be used during hybridization and washing to achieve maximum specific binding to the target sequence.
- the washes following hybridization are performed at increasing degrees of stringency to remove non-hybridized polynucleotide probes from hybridized complexes.
- the above conditions are meant to serve as a guide and it is well within the abilities of one skilled in the art to adapt these conditions for use with a particular polypeptide hybrid.
- the T m for a specific target sequence is the temperature (under defined conditions) at which 50% of the target sequence will hybridize to a perfectly matched probe sequence.
- Those conditions which influence the T ⁇ include, the size and base pair content of the polynucleotide probe, the ionic strength of the hybridization solution, and the presence of destabilizing agents in the hybridization solution.
- OLIGO 6.0 LSR; Long Lake, MN
- Primer Premier 4.0 Premier Biosoft International; Palo Alto, CA
- sites on the Internet are available tools for analyzing a given sequence and calculating T m based on user defined criteria. Such programs can also analyze a given sequence under defined conditions and identify suitable probe sequences. Typically, hybridization of longer polynucleotide sequences,
- >50 base pairs is performed at temperatures of about 20-25°C below the calculated T m .
- hybridization is typically carried out at the T m or 5- 10°C below. This allows for the maximum rate of hybridization for DNA-DNA and DNA-RNA hybrids.
- the length of the polynucleotide sequence influences the rate and stability of hybrid formation. Smaller probe sequences, ⁇ 50 base pairs, reach equilibrium with complementary sequences rapidly, but may form less stable hybrids. Incubation times of anywhere from minutes to hours can be used to achieve hybrid formation. Longer probe sequences come to equilibrium more slowly, but form more stable complexes even at lower temperatures. Incubations are allowed to proceed overnight or longer.
- incubations are carried out for a period equal to three times the calculated Cot time.
- Cot time the time it takes for the polynucleotide sequences to reassociate, can be calculated for a particular sequence by methods known in the art.
- the base pair composition of polynucleotide sequence will effect the thermal stability of the hybrid complex, thereby influencing the choice of hybridization temperature and the ionic strength of the hybridization buffer.
- A-T pairs are less stable than G-C pairs in aqueous solutions containing sodium chloride. Therefore, the higher the G-C content, the more stable the hybrid. Even distribution of G and C residues within the sequence also contribute positively to hybrid stability.
- the base pair composition can be manipulated to alter the T, bookmark of a given sequence.
- 5-methyldeoxycytidine can be substituted for deoxycytidine and 5-bromodeoxuridine can be substituted for thymidine to increase the T n
- 7-deazz-2'-deoxyguanosine can be substituted for guanosine to reduce dependence on T m .
- Hybridization buffers generally contain blocking agents such as Denhardt's solution (Sigma Chemical Co., St. Louis, Mo.), denatured salmon sperm DNA, tRNA, milk powders (BLOTTO), heparin or SDS, and a Na + source, such as SSC (lx SSC: 0.15 M sodium chloride, 15 mM sodium citrate) or SSPE ( lx SSPE: 1.8 M NaCI, 10 mM NaH 2 P0 4 , 1 mM EDTA, pH 7.7).
- SSC Denhardt's solution
- BLOTTO denatured salmon sperm DNA
- tRNA milk powders
- BLOTTO milk powders
- heparin or SDS heparin or SDS
- Na + source such as SSC (lx SSC: 0.15 M sodium chloride, 15 mM sodium citrate) or SSPE ( lx SSPE: 1.8 M NaCI, 10 mM NaH 2 P0 4 , 1 mM
- hybridization buffers typically contain from between 10 mM - 1 M Na + .
- destabilizing or denaturing agents such as formamide, tetralkylammonium salts, guanidinium cations or thiocyanate cations to the hybridization solution will alter the T m of a hybrid.
- formamide is used at a concentration of up to 50% to allow incubations to be carried out at more convenient and lower temperatures. Formamide also acts to reduce non-specific background when using RNA probes.
- the isolated polynucleotides of the present invention include DNA and RNA.
- Methods for preparing DNA and RNA are well known in the art.
- RNA is isolated from a tissue or cell that produces large amounts of zFGF12 RNA. Such tissues and cells are identified by Northern blotting (Thomas, Proc. Natl. Acad. Sci. USA 77:5201, 1980), and include pancreas and prostate.
- Total RNA can be prepared using guanidinium isothiocyanate extraction followed by isolation by centrifugation in a CsCl gradient (Chirgwin et al., Biochemistry 18:52-94, 1979).
- Poly (A)+ RNA is prepared from total RNA using the method of Aviv and Leder (Proc.
- cDNA Complementary DNA
- Polynucleotides encoding zFGF12 polypeptides are then identified and isolated by, for example, hybridization or PCR.
- a full-length clone encoding zFGF12 can be obtained by conventional cloning procedures.
- Complementary DNA (cDNA) clones are preferred, although for some applications (e.g., expression in transgenic animals) it may be preferable to use a genomic clone, or to modify a cDNA clone to include at least one genomic intron.
- Methods for preparing cDNA and genomic clones are well known and within the level of ordinary skill in the art, and include the use of the sequence disclosed herein, or parts thereof, for probing or priming a library.
- Expression libraries can be probed with antibodies to zFGF12, receptor fragments, or other specific binding partners.
- the present invention further provides counte ⁇ art polypeptides and polynucleotides from other species (orthologs).
- zFGF12 polypeptides from other mammalian species, including murine, rat, porcine, ovine, bovine, canine, feline, equine and other primate proteins.
- Orthologs of the human proteins can be cloned using information and compositions provided by the present invention in combination with conventional cloning techniques.
- a cDNA can be cloned using mRNA obtained from a tissue or cell type that expresses the protein. Suitable sources of mRNA can be identified by probing Northern blots with probes designed from the sequences disclosed herein.
- a library is then prepared from mRNA of a positive tissue or cell line.
- a zFGF12-encoding cDNA can then be isolated by a variety of methods, such as by probing with a complete or partial human cDNA or with one or more sets of degenerate probes based on the disclosed sequences.
- a cDNA can also be cloned using the polymerase chain reaction, or PCR (Mullis, U.S. Patent 4,683,202), using primers designed from the sequences disclosed herein.
- the cDNA library can be used to transform or transfect host cells, and expression of the cDNA of interest can be detected with an antibody to zFGF12. Similar techniques can also be applied to the isolation of genomic clones.
- SEQ ID NO: 1 and SEQ ID NO: 2 represent a single allele of the human zFGF12 gene and polypeptide, respectively, and that allelic variation and alternative splicing are expected to occur.
- Allelic variants can be cloned by probing cDNA or genomic libraries from different individuals according to standard procedures. Allelic variants of the DNA sequence shown in SEQ ED NO: 1, including those containing silent mutations and those in which mutations result in amino acid sequence changes, are within the scope of the present invention, as are proteins which are allelic variants of SEQ ID NO: 2.
- Mutagenesis methods as disclosed above can be combined with high- throughput, automated screening methods to detect activity of cloned, mutagenized polypeptides in host cells.
- Mutagenized DNA molecules that encode active polypeptides e.g., cell proliferation
- These methods allow the rapid determination of the importance of individual amino acid residues in a polypeptide of interest, and can be applied to polypeptides of unknown structure.
- polypeptides that are substantially homologous to residues 25 (Tyr) to 251 (He) or residues 1 (Met) to 251 (He) of SEQ ID NO: 2, allelic variants thereof, or biologically active fragments thereof, and retain the proliferative properties of the wild-type protein.
- Such polypeptides may also include additional polypeptide segments as generally disclosed above.
- the polypeptides of the present invention including full-length proteins, fragments thereof and fusion proteins, can be produced in genetically engineered host cells according to conventional techniques.
- Suitable host cells are those cell types that can be transformed or transfected with exogenous DNA and grown in culture, and include bacteria, fungal cells, and cultured higher eukaryotic cells. Eukaryotic cells, particularly cultured cells of multicellular organisms, are preferred. Techniques for manipulating cloned DNA molecules and introducing exogenous DNA into a variety of host cells are disclosed by Sambrook et al., Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989, and Ausubel et al. (eds.), Current Protocols in Molecular Biology, John Wiley and Sons, Inc., NY, 1987, which are inco ⁇ orated herein by reference.
- a DNA sequence encoding a zFGF12 polypeptide of the present invention is operably linked to other genetic elements required for its expression, generally including a transcription promoter and terminator within an expression vector.
- the vector will also commonly contain one or more selectable markers and one or more origins of replication, although those skilled in the art will recognize that within certain systems selectable markers may be provided on separate vectors, and replication of the; exogenous DNA may be provided by integration into the host cell genome. Selection of promoters, terminators, selectable markers, vectors and other elements is a matter of routine design within the level of ordinary skill in the art. Many such elements are described in the literature and are available through commercial suppliers.
- Alternative markers that introduce an altered phenotype such as green fluorescent protein, or cell surface proteins such as CD4, CD8, Class I MHC, placental alkaline phosphatase may be used to sort transfected cells from untransfected cells by such means as FACS sorting or magnetic bead separation technology.
- a secretory signal sequence (also known as a leader sequence, prepro sequence or pre sequence) is provided in the expression vector.
- the secretory signal sequence may be the native sequence, or a chimera comprising a signal sequence derived from another secreted protein (e.g., t-PA and ⁇ -pre-pro secretory leader) or synthesized de novo.
- the secretory signal sequence is joined to the zFGF12 DNA sequence in the correct reading frame.
- Secretory signal sequences are commonly positioned 5' to the DNA sequence encoding the polypeptide of interest, although certain signal sequences may be positioned elsewhere in the DNA sequence of interest (see, e.g., Welch et al., U.S. Patent No. 5,037,743; Holland et al., U.S. Patent No. 5,143,830).
- the secretory signal sequence contained in the polypeptides of the present invention is used to direct other polypeptides into the secretory pathway.
- the present invention provides for such fusion polypeptides.
- a signal fusion polypeptide can be made wherein a secretory signal sequence derived from amino acid residues 1-24 of SEQ ID NO:2 is be operably linked to another polypeptide using methods known in the art and disclosed herein.
- the secretory signal sequence contained in the fusion polypeptides of the present invention is preferably fused amino-terminally to an additional peptide to direct the additional peptide into the secretory pathway.
- Such constructs have numerous applications known in the art.
- these novel secretory signal sequence fusion constructs can direct the secretion of an active component of a normally non-secreted protein.
- Such fusions may be used in vivo or in vitro to direct peptides through the secretory pathway.publication
- Insect cells can be infected with recombinant baculovirus, commonly derived from Autographa californica nuclear polyhedrosis virus (AcNPV).
- AcNPV Autographa californica nuclear polyhedrosis virus
- a second method of making recombinant zFGF12 baculovirus utilizes a transposon-based system described by Luckow (Luckow, V.A, et al., J Virol 67:4566-79, 1993).
- This system which utilizes transfer vectors, is sold in the Bac-to- BacTM kit (Life Technologies, Rockville, MD).
- This system utilizes a transfer vector, pFastBadTM (Life Technologies) containing a Tn7 transposon to move the DNA encoding the zFGF12 polypeptide into a baculovirus genome maintained in E. coli as a large plasmid called a "bacmid.” See, Hill-Perkins, M.S.
- transfer vectors can include an in-frame fusion with DNA encoding an epitope tag at the C- or N- terminus of the expressed zFGF12 polypeptide, for example, a Glu-Glu epitope tag (Grussenmeyer, T. et al., Proc. Natl. Acad. Sci. 82:7952-4, 1985).
- a transfer vector containing zFGF12 is transformed into E. coli, and screened for bacmids which contain an interrupted lacZ gene indicative of recombinant baculovirus.
- the bacmid DNA containing the recombinant baculovirus genome is isolated, using common techniques, and used to transfect Spodoptera frugiperda cells, e.g. Sf9 cells.
- Recombinant virus that expresses zFGF12 is subsequently produced.
- Recombinant viral stocks are made by methods commonly used the art.
- the recombinant virus is used to infect host cells, typically a cell line derived from the fall armyworm, Spodoptera frugiperda. See, in general, Glick and Pasternak, Molecular Biotechnology: Principles and Applications of Recombinant DNA, ASM Press, Washington, D.C, 1994.
- Another suitable cell line is the High FiveOTM cell line (Invitrogen) derived from Trichoplusia ni (U.S. Patent No. 5,300,435).
- Commercially available serum-free media are used to grow and maintain the cells.
- Suitable media are Sf900 IF M (Life Technologies) or ESF 921TM (Expression Systems) for the Sf9 cells; and Ex-cellO405TM (JRH Biosciences, Lenexa, KS) or Express FiveOTM (Life Technologies) for the T. ni cells.
- the cells are grown up from an inoculation density of approximately 2-5 x 10 5 cells to a density of 1-2 x 10 ' cells at which time a recombinant viral stock is added at a multiplicity of infection (MOI) of 0.1 to 10, more typically near 3.
- MOI multiplicity of infection
- Fungal cells including yeast cells, can also be used within the present invention.
- Yeast species of particular interest in this regard include Saccharomyces cerevisiae, Pichia pastoris, and Pichia methanolica.
- Methods for transforming S. cerevisiae cells with exogenous DNA and producing recombinant polypeptides therefrom are disclosed by, for example, Kawasaki, U.S. Patent No. 4,599,31 1; Kawasaki et al., U.S. Patent No. 4,931,373; Brake, U.S. Patent No. 4,870,008; Welch et al., U.S. Patent No. 5,037,743; and Murray et al., U.S. Patent No. 4,845,075.
- Transformed cells are selected by phenotype determined by the selectable marker, commonly drug resistance or the ability to grow in the absence of a particular nutrient (e.g., leucine).
- a preferred vector system for use in Saccharomyces cerevisiae is the POT1 vector system disclosed by Kawasaki et al. (U.S. Patent No. 4,931,373), which allows transformed cells to be selected by growth in glucose-containing media.
- Suitable promoters and terminators for use in yeast include those from glycolytic enzymes (Cregg, U.S. Patent No. 4,882,279.) Aspergillus cells may be utilized according to the methods of McKnight et al., U.S. Patent No. 4,935,349.
- Pichia methanolica as host for the production of recombinant proteins is disclosed in WIPO Publications WO 97/17450, WO 97/17451, WO 98/02536, and WO 98/02565.
- DNA molecules for use in transforming P. methanolica will commonly be prepared as double-stranded, circular plasmids, which are preferably linearized prior to transformation.
- the promoter and terminator in the plasmid be that of a P. methanolica gene, such as a P. methanolica alcohol utilization gene (AUG1 or AUG2).
- DHAS dihydroxyacetone synthase
- FMD formate dehydrogenase
- CAT catalase
- a preferred selectable marker for use in Pichia methanolica is a P. methanolica ADE2 gene, which encodes phosphoribosyl-5-aminoimidazole carboxylase (AIRC; EC 4.1.1.21), which allows ade2 host cells to grow in the absence of adenine.
- host cells For large-scale, industrial processes where it is desirable to minimize the use of methanol, it is preferred to use host cells in which both methanol utilization genes (AUG1 and AUG2) are deleted. For production of secreted proteins, host cells deficient in vacuolar protease genes (PEP4 and PRB1) are preferred. Electroporation is used to facilitate the introduction of a plasmid containing DNA encoding a polypeptide of interest into P. methanolica cells. It is preferred to transform P.
- methanolica cells by electroporation using an exponentially decaying, pulsed electric field having a field strength of from 2.5 to 4.5 kV/cm, preferably about 3.75 kV/cm, and a time constant (t) of from 1 to 40 milliseconds, most preferably about 20 milliseconds.
- Cultured mammalian cells are suitable hosts within the present invention.
- Methods for introducing exogenous DNA into mammalian host cells include calcium phosphate-mediated transfection (Wigler et al., Cell 14:725, 1978; Corsaro and Pearson, Somatic Cell Genetics 7:603, 1981 : Graham and Van der Eb, Virology 52:456, 1973), electroporation (Neumann et al., EMBO J.
- Suitable cultured mammalian cells include the COS-1 (ATCC No. CRL 1650), COS-7 (ATCC No. CRL 1651 ), BHK (ATCC No. CRL 1632), BHK 570 (ATCC No. CRL 10314), 293 (ATCC No. CRL 1573; Graham et al., J. Gen. Virol. 36:59-72, 1977) and Chinese hamster ovary (e.g. CHO-K1 ; ATCC No. CCL 61 or DG44) cell lines.
- Suitable cell lines are known in the art and available from public depositories such as the American Type Culture Collection, Rockville, Maryland.
- strong transcription promoters are preferred, such as promoters from SV-40 or cytomegalovirus. See, e.g., U.S. Patent No. 4,956,288.
- Other suitable promoters include those from metallothionein genes (U.S. Patent Nos. 4,579,821 and 4,601, 978)and the adenovirus major late promoter.
- Drug selection is generally used to select for cultured mammalian cells into which foreign DNA has been inserted. Such cells are commonly referred to as
- transfectants Cells that have been cultured in the presence of the selective agent and are able to pass the gene of interest to their progeny are referred to as “stable transfectants.”
- a preferred selectable marker is a gene encoding resistance to the antibiotic neomycin. Selection is carried out in the presence of a neomycin-type drug, such as G-418 or the like. Selection systems can also be used to increase the expression level of the gene of interest, a process referred to as "amplification.” Amplification is carried out by culturing transfectants in the presence of a low level of the selective agent and then increasing the amount of selective agent to select for cells that produce high levels of the products of the introduced genes.
- a preferred amplifiable selectable marker is dihydrofolate reductase, which confers resistance to methotrexate.
- Other drug resistance genes e.g. hygromycin resistance, multi-drug resistance, puromycin acetyltransferase
- hygromycin resistance e.g. hygromycin resistance, multi-drug resistance, puromycin acetyltransferase
- Agrobacterium rhizogenes as a vector for expressing genes in plant cells has been reviewed by Sinkar et al., J. Biosci. (Bangalore)
- Transformed or transfected host cells are cultured according to conventional procedures in a culture medium containing nutrients and other components required for the growth of the chosen host cells.
- suitable media including defined media and complex media, are known in the art and generally include a carbon source, a nitrogen source, essential amino acids, vitamins and minerals. Media may also contain such components as growth factors or serum, as required.
- the growth medium will generally select for cells containing the exogenously added DNA by, for example, drug selection or deficiency in an essential nutrient which is complemented by the selectable marker carried on the expression vector or co- transfected into the host cell.
- P. methanolica cells are cultured in a medium comprising adequate sources of carbon, nitrogen and trace nutrients at a temperature of about 25°C to 35°C.
- Liquid cultures are provided with sufficient aeration by conventional means, such as shaking of small flasks or sparging of fermentors.
- a preferred culture medium for P. methanolica is YEPD (2% D-glucose, 2% BactoTM Peptone (Difco Laboratories, Detroit, MI), 1% BactoTM yeast extract (Difco Laboratories), 0.004% adenine and 0.006% L-leucine).
- polypeptides of the present invention it is preferred to purify the polypeptides of the present invention to >80% purity, more preferably to >90% purity, even more preferably >95% purity, and particularly preferred is a pharmaceutically pure state, that is greater than 99.9% pure with respect to contaminating macromolecules, particularly other proteins and nucleic acids, and free of infectious and pyrogenic agents.
- a purified polypeptide is substantially free of other polypeptides, particularly other polypeptides of animal origin.
- Expressed recombinant zFGF12 polypeptides (or chimeric zFGF12 polypeptides) can be purified using fractionation and/or conventional purification methods and media. Ammonium sulfate precipitation and acid or chaotrope extraction may be used for fractionation of samples.
- Exemplary purification steps may include hydroxyapatite, size exclusion, FPLC and reverse-phase high performance liquid chromatography.
- Suitable anion exchange media include derivatized dextrans, agarose, cellulose, polyacrylamide, specialty silicas, and the like. PEI, DEAE, QAE and Q derivatives are preferred, with DEAE Fast-Flow Sepharose (Pharmacia, Piscataway, NJ) being particularly preferred.
- Exemplary chromatographic media include those media derivatized with phenyl, butyl, or octyl groups, such as Phenyl-Sepharose FF (Pharmacia), Toyopearl butyl 650 (Toso Haas, Montgomeryville, PA), Octyl-Sepharose (Pharmacia) and the like; or polyacrylic resins, such as Amberchrom CG 71 (Toso Haas) and the like.
- Suitable solid supports include glass beads, silica-based resins, cellulosic resins, agarose beads, cross-linked agarose beads, polystyrene beads, cross- linked polyacrylamide resins and the like that are insoluble under the conditions in which they are to be used.
- These supports may be modified with reactive groups that allow attachment of proteins by amino groups, carboxyl groups, sulfhydryl groups, hydroxyl groups and/or carbohydrate moieties.
- Examples of coupling chemistries include cyanogen bromide activation, N-hydroxysuccinimide activation, epoxide activation, sulfhydryl activation, hydrazide activation, and carboxyl and amino derivatives for carbodiimide coupling chemistries.
- These and other solid media are well known and widely used in the art, and are available from commercial suppliers. Methods for binding receptor polypeptides to support media are well known in the art. Selection of a particular method is a matter of routine design and is determined in part by the properties of the chosen support.
- polypeptides of the present invention can also be isolated by exploitation of their heparin binding properties.
- Members of the FGF family can be purified to apparent homogeneity by heparin-Sepharose affinity chromatography (Gospodarowicz et al., Proc. Natl. Acad. Sci. 81:6963-6967, 1984) and eluted using linear step gradients of NaCI (Ron et al., J. Biol. Chem. 268(4):2984-2988, 1993; Chromatography:
- a gel is first charged with divalent metal ions to form a chelate (E. Sulkowski, Trends in Biochem. 3: 1-7, 1985). Histidine-rich proteins will be adsorbed to this matrix with differing affinities, depending upon the metal ion used, and will be eluted by competitive elution, lowering the pH, or use of strong chelating agents.
- Other methods of purification include purification of glycosylated proteins by lectin affinity chromatography and ion exchange chromatography (Methods in Enzymol., Vol. 182, "Guide to Protein Purification", M. Lieber, (ed.), Acad. Press, San Diego, 1990, pp.529-39).
- an affinity tag e.g., polyhistidine, maltose-binding protein, an immunoglobulin domain
- Protein refolding (and optionally reoxidation) procedures may be advantageously used. It is preferred to purify the protein to >80% purity, more preferably to >90% purity, even more preferably >95%, and particularly preferred is a pharmaceutically pure state, that is greater than 99.9% pure with respect to contaminating macromolecules, particularly other proteins and nucleic acids, and free of infectious and pyrogenic agents. Preferably, a purified protein is substantially free of other proteins, particularly other proteins of animal origin.
- zFGF12 polypeptides or fragments thereof may also be prepared through chemical synthesis. zFGF12 polypeptides may be monomers or multimers; glycosylated or non-glycosylated; pegylated or non-pegylated; and may or may not include an initial methionine amino acid residue.
- viruses for this pu ⁇ ose include adenovirus, he ⁇ esvirus, vaccinia virus and adeno-associated virus (AAV).
- Adenovirus a double- stranded DNA virus, is currently the best studied gene transfer vector for delivery of heterologous nucleic acid (for a review, see T.C. Becker et al., Meth. Cell Biol. 43: 161- 89, 1994; and J.T. Douglas and D.T. Curiel, Science & Medicine 4:44-53, 1997).
- adenovirus can (i) accommodate relatively large DNA inserts; (ii) be grown to high-titer; (iii) infect a broad range of mammalian cell types; and (iv) be used with a large number of available vectors containing different promoters. Also, because adenoviruses are stable in the bloodstream, they can be administered by intravenous injection.
- inserts up to 7 kb of heterologous DNA can be accommodated. These inserts can be inco ⁇ orated into the viral DNA by direct ligation or by homologous recombination with a co- transfected plasmid.
- the essential El gene has been deleted from the viral vector, and the virus will not replicate unless the El gene is provided by the host cell (the human 293 cell line is exemplary).
- the host cell the human 293 cell line is exemplary.
- adenovirus primarily targets the liver. If the adenoviral delivery system has an El gene deletion, the virus cannot replicate in the host cells.
- the host's tissue e.g., liver
- the host's tissue will express and process (and, if a secretory signal sequence is present, secrete) the heterologous protein.
- Secreted proteins will enter the circulation in the highly vascularized liver, and effects on the infected animal can be determined.
- the adenovirus system can also be used for protein production in vitro.
- the cells By culturing adenovirus-infected non-293 cells under conditions where the cells are not rapidly dividing, the cells can produce proteins for extended periods of time. For instance, BHK cells are grown to confluence in cell factories, then exposed to the adenoviral vector encoding the secreted protein of interest. The cells are then grown under serum-free conditions, which allows infected cells to survive for several weeks without significant cell division.
- adenovirus vector infected 293 cells can be grown as adherent cells or in suspension culture at relatively high cell density to produce significant amounts of protein (see Garnier et al., Cytotechnol. 15: 145-55. 1994). With either protocol, an expressed, secreted heterologous protein can be repeatedly isolated from the cell culture supernatant. Within the infected 293S cell production protocol, non-secreted proteins may also be effectively obtained.
- the activity of molecules of the present invention can be measured using a variety of assays that, for example, measure neogenesis or hype ⁇ lasia (i.e., proliferation) of neuronal, prostatic, renal, or pancreatic cells based on the tissue specificity.
- tissue analysis supports that zFGF12 promotes growth and/or differentiation of hematopoietic cells or stromal cells supporting growth of hematopoietic cells.
- Additional activities likely associated with the polypeptides of the present invention include proliferation of endothelial cells, fibroblasts, cardiac and skeletal myocytes, epithelial cells and keratinocytes, directly or indirectly through other growth factors; action as a chemotaxic factor for endothelial cells, fibroblasts and/or phagocytic cells; osteogenic factor; and factor for expanding mesenchymal stem cell and precursor populations.
- Proliferation can be measured using cultured cardiac cells or in vivo by administering molecules of the claimed invention to the appropriate animal model. Generally, proliferative effects are seen as an increase in cell number and therefore, may include inhibition of apoptosis, as well as mitogenesis.
- Cultured cells include fibroblasts, skeletal myocytes, human umbilical vein endothelial cells from primary cultures. Established cell lines include: Nffl 3T3 fibroblast (ATCC No. CRL- 1658), CHH-1 chum heart cells (ATCC No. CRL- 1680), H9c2 rat heart myoblasts (ATCC No. CRL-1446), Shionogi mammary carcinoma cells (Tanaka et al., Proc. Natl.
- Assays measuring cell proliferation are well known in the art. For example, assays measuring proliferation incl ide such assays as chemosensitivity to neutral red dye (Cavanaugh et al., Investigational New Drugs 8:347-354, 1990, inco ⁇ orated herein by reference), inco ⁇ oration of radiolabelled nucleotides (Cook et al., Analytical Biochem.
- Differentiation is a progressive and dynamic process, beginning with pluripotent stem cells and ending with terminally differentiated cells.
- Pluripotent stem cells that can regenerate without commitment to a lineage express a set of differentiation markers that are lost when commitment to a cell lineage is made.
- Progenitor cells express a set of differentiation markers that may or may not continue to be expressed as the cells progress down the cell lineage pathway toward maturation.
- Differentiation markers that are expressed exclusively by mature cells are usually functional properties such as cell products, enzymes to produce cell products and receptors. The stage of a cell population's differentiation is monitored by identification of markers present in the cell population.
- Myocytes, osteoblasts, adipocytes, chrondrocytes, fibroblasts and reticular cells are believed to originate from a common mesenchymal stem cell (Owen et al., Ciba Fdn. Symp. 136:42-46, 1988). Markers for mesenchymal stem cells have not been well identified (Owen et al., J. of Cell Sci. 87:731-738, 1987), so identification is usually made at the progenitor and mature cell stages.
- the novel polypeptides of the present invention are useful for studies to isolate mesenchymal stem cells and myocyte progenitor cells, both in vivo and ex vivo.
- the present invention includes stimulation, inhibition, or proliferation of myocytes, smooth muscle cells, osteoblasts, adipocytes, chondrocytes, neural tube-derived stem cells, neural crest stem cells, and neuronal progenitors, pancreatic cells, prostate-derived cells and endothelial cells.
- Molecules of the present invention may, while stimulating proliferation or differentiation of cardiac myocytes, inhibit proliferation or differentiation of adipocytes, by virtue of the affect on their common precursor/stem cells.
- molecules of the present invention have use in inhibiting chondrosarcomas, atherosclerosis, restenosis and obesity.
- Assays measuring differentiation include, for example, measuring cell- surface markers associated with stage-specific expression of a tissue, enzymatic activity, functional activity or mo ⁇ hological changes (Watt, FASEB, 5:281-284, 1991 ; Francis, Differentiation 57:63-75, 1994; Raes, Adv. Anim. Cell Bio). Technol. Bioprocesses, 161-171, 1989; all inco ⁇ orated herein by reference).
- In vivo assays for evaluating neogenesis or hype ⁇ lasia include cellular proliferation assays (Stern et al., Proc. Natl. Acad. Sci. 87:6808-6812. 1990, Lok et al., Nature 369:565-568, 1994), stimulation of the proliferation of neuronal and glial progenitors isolated from the septum and striatum (Palmer et al., Mol. Cell. Neurosci. 6:474-486, 1995), and stimulation of differentiation of neurons from neural crest progenitors (Vaisman et al., Development 1 15: 1059-1069, 1992).
- In vivo assays for measuring changes in bone formation rates include performing bone histology (see, Recker, R., eds. Bone Histomo ⁇ hometry: Techniques and Inte ⁇ retation. Boca Raton: CRC Press, Inc., 1983) and quantitative computed tomography (QCT; Ferretti,J. Bone !_7:353S-364S, 1995; O ⁇ hanoludakis et al., Investig. Radiol. 14: 122-130,, 1979 and Durand et al., Medical Physics 19:569-573, 1992).
- An ex vivo assay for measuring changes in bone formation would be, for example, a calavarial assay (Gowen et al., J. Immunol. 136:2478-2482, 1986).
- zFGF12 polypeptides may modulate effects on metabolic reactions.
- Such metabolic reactions include adipogenesis, gluconeogenesis, glycogenolysis, lipogenesis, glucose uptake, protein synthesis, thermogenesis, oxygen utilization and the like.
- mammalian energy balance may be evaluated by monitoring one or more of the aforementioned metabolic functions. These metabolic functions are monitored by techniques (assays or animal models) known to one of ordinary skill in the art, as is more fully set forth below.
- the glucoregulatory effects of insulin are predominantly exerted in the liver, skeletal muscle and adipose tissue. In skeletal muscle and adipose tissue, insulin acts to stimulate the uptake, storage and utilization of glucose.
- Insulin-stimulated lipogenesis may be monitored by measuring the inco ⁇ oration of 14c_ ace t a t e into triglyceride (Mackall et al. J. Biol. Chem. 251:6462-6464, 1976) or triglyceride accumulation (Kletzien et al., Mol. Pharmacol. 41:393-398, 1992).
- zFGF12-stimulated uptake may be evaluated, for example, in an assay for insulin-stimulated glucose transport.
- Primary adipocytes or NIH 3T3 LI cells (ATCC No. CCL-92.1) are placed in DMEM containing 1 g/1 glucose, 0.5 or 1.0% BSA, 20 mM Hepes, and 2 mM glutamine. After two to five hours of culture, the medium is replaced with fresh, glucose-free DMEM containing 0.5 or 1.0% BSA, 20 mM Hepes, 1 mM pyruvate, and 2 mM glutamine.
- zFGF12 concentrations of zFGF12, insulin or IGF-1, or a dilution series of the test substance, are added, and the cells are incubated for 20-30 minutes.
- the cells are then quickly rinsed with cold buffer (e.g. PBS), then lysed with a suitable lysing agent (e.g. 1% SDS or 1 N NaOH). The cell lysate is then evaluated by counting in a scintillation counter.
- a suitable lysing agent e.g. 1% SDS or 1 N NaOH
- Cell-associated radioactivity is taken as a measure of glucose transport after subtracting non-specific binding as determined by incubating cells in the presence of cytochalasin b, an inhibitor of glucose transport.
- Other methods include those described by, for example, Manchester et al., Am. J. Physiol. 266 (Endocrinol. Metab. 29):E326-E333, 1994 (insulin-stimulated glucose transport).
- Protein synthesis may be evaluated, for example, by comparing precipitation of 35 S-methionine-labeled proteins following incubation of the test cells with s S-methionine and 35 S-methionine and a putative modulator of protein synthesis.
- Thermogenesis may be evaluated as described by B. Stanley in The Biology of Neuropeptide Y and Related Peptides, W. Colmers and C. Wahlestedt (eds.),
- metabolic rate which may be measured by a variety of techniques, is an indirect measurement of thermogenesis.
- Oxygen utilization may be evaluated as described by Heller et al.,
- zFGF12 polypeptides can also be used to prepare antibodies that specifically bind to zFGF12 epitopes, peptides or polypeptides.
- Methods for preparing polyclonal and monoclonal antibodies are well known in the art (see, for example, Sambrook et al., Molecular Cloning: A Laboratory Manual, Second Edition, Cold Spring Harbor, NY, 1989; and Hurrell, J. G. R., Ed., Monoclonal Hybridoma Antibodies: Techniques and Applications, CRC Press, Inc., Boca Raton, FL, 1982, which are inco ⁇ orated herein by reference).
- polyclonal antibodies can be generated from a variety of warm-blooded animals, such as horses, cows, goats, sheep, dogs, chickens, rabbits, mice, and rats.
- the immunogenicity of a zFGF12 polypeptide may be increased through the use of an adjuvant, such as alum (aluminum hydroxide) or Freund's complete or incomplete adjuvant.
- Polypeptides useful for immunization also include fusion polypeptides, such as fusions of zFGF12 or a portion thereof with an immunoglobulin polypeptide or with maltose binding protein.
- the polypeptide immunogen may be a full-length molecule or a portion thereof.
- polypeptide portion is "hapten-like"
- such portion may be advantageously joined or linked to a macromolecular carrier (such as keyhole limpet hemocyanin (KLH), bovine serum albumin (BSA) or tetanus toxoid) for immunization.
- a macromolecular carrier such as keyhole limpet hemocyanin (KLH), bovine serum albumin (BSA) or tetanus toxoid
- antibodies includes polyclonal antibodies, affinity-purified polyclonal antibodies, monoclonal antibodies, and antigen-binding fragments, such as F(ab')2 and Fab proteolytic fragments. Genetically engineered intact antibodies or fragments, such as chimeric antibodies, Fv fragments, single chain antibodies and the like, as well as synthetic antigen-binding peptides and polypeptides, are also included.
- Non-human antibodies may be humanized by grafting only non- human CDRs onto human framework and constant regions, or by inco ⁇ orating the entire non-human variable domains (optionally "cloaking" them with a human-like surface by replacement of exposed residues, wherein the result is a "veneered” antibody).
- humanized antibodies may retain non-human residues within the human variable region framework domains to enhance proper binding characteristics. Through humanizing antibodies, biological half-life may be increased, and the potential for adverse immune reactions upon administration to humans is reduced.
- Alternative techniques for generating or selecting antibodies useful herein include in vitro exposure of lymphocytes to zFGF12 protein or peptide, and selection of antibody display libraries in phage or similar vectors (for instance, through use of immobilized or labeled zFGF12 protein or peptide).
- Antibodies ar2 defined to be specifically binding if they bind to a zFGF12 polypeptide with a binding affinity (K a ) of 10 M " or greater, preferably 10 M or greater, more preferably 10 M " or greater, and most preferably 10 M or greater.
- K a binding affinity
- the binding affinity of an antibody can be readily determined by one of ordinary skill in the art (for example, by Scatchard analysis).
- assays known to those skilled in the art can be utilized to detect antibodies which specifically bind to zFGF12 proteins or peptides. Exemplary assays are described in detail in Antibodies: A Laboratory Manual, Harlow and Lane (Eds.), Cold Spring Harbor Laboratory Press, 1988. Representative examples of such assays include: concurrent immunoelectrophoresis, radioimmunoassay, radioimmuno- precipitation, enzyme-linked immunosorbent assay (ELISA), dot blot or Western blot assay, inhibition or competition assay, and sandwich assay. In addition, antibodies can be screened for binding to wild-type versus mutant zFGF12 protein or peptide.
- Antibodies to zFGF12 may be used for tagging cells that express zFGF12; to target another protein, small molecule or chemical to heart tissue; for isolating zFGF12 by affinity purification; for diagnostic assays for determining circulating levels of zFGF12 polypeptides; for detecting or quantitating soluble zFGF12 as marker of underlying pathology or disease; in analytical methods employing FACS; for screening expression libraries; for generating anti-idiotypic antibodies; and as neutralizing antibodies or as antagonists to block zFGF12 mediated proliferation in vitro and in vivo.
- Suitable direct tags or labels include radionuclides, enzymes, substrates, cofactors, inhibitors, fluorescent markers, chemiluminescent markers, magnetic particles and the like; indirect tags or labels may feature use of biotin-avidin or other complement/anti-complement pairs as intermediates.
- Antibodies herein may also be directly or indirectly conjugated to drugs, toxins, radionuclides and the like, and these conjugates used for in vivo diagnostic or therapeutic applications.
- Molecules of the present invention can be used to identify and isolate receptors involved in neuronal or pancreatic cell proliferation.
- proteins and peptides of the present invention can be immobilized on a column and membrane preparations run over the column (Immobilized Affinity Ligand Techniques,
- Proteins and peptides can also be radiolabeled (Methods in EnzymoL, vol. 182, "Guide to Protein Purification", M. Deutscher, ed., Acad. Press, San Diego, 1990, 721-737) or photoaffinity labeled (Brunner et al., Ann. Rev. Biochem. 62:483-514, 1993 and Fedan et al., Biochem. Pharmacol. 33:1167-1180, 1984) and specific cell-surface proteins can be identified.
- Antagonists will be useful for inhibiting the proliferative activities of zFGF12 molecules, in cell types such as neuronal, pancreatic, epithelial cells, keratinocytes, and prostatic cells, including fibroblasts and endothelial cells.
- antagonists to zFGF12 will be useful for inhibitions of disorders associated with kidney epithelium, such as glomerulonephritis.
- disorders associated with keratinocytes, such as psoriasis may be inhibited by zFGF12 antagonists.
- Genes encoding zFGF12 polypeptide binding domains can be obtained by screening random peptide libraries displayed on phage (phage display) or on bacteria, such as E. coli.
- Nucleotide sequences encoding the polypeptides can be obtained in a number of ways, such as through random mutagenesis and random polynucleotide synthesis. These random peptide display libraries can be used to screen for peptides which interact with a known target which can be a protein or polypeptide, such as a ligand or receptor, a biological or synthetic macromolecule, or organic or inorganic substances.
- a known target can be a protein or polypeptide, such as a ligand or receptor, a biological or synthetic macromolecule, or organic or inorganic substances.
- Random peptide display libraries can be screened using the zFGF12 sequences disclosed herein to identify proteins which bind to zFGF12.
- These "binding proteins" which interact with zFGF12 polypeptides may be used for tagging cells; for isolating homolog polypeptides by affinity purification; they can be directly or indirectly conjugated to drugs, toxins, radionuclides and the like.
- binding proteins can also be used in analytical methods such as for screening expression libraries and neutralizing activity.
- the binding proteins can also be used for diagnostic assays for determining circulating levels of polypeptides; for detecting or quantitating soluble polypeptides as marker of underlying pathology or disease.
- binding proteins can also act as zFGF12 "antagonists" to block zFGF12 binding and signal transduction in vitro and in vivo. These anti-zFGF12 binding proteins would be useful for inhibiting expression of genes which result in proliferation or differentiation. Such anti-zFGF12 binding proteins can be used for treatment, for example, in neuroblastoma, glioblastoma, prostatic hypertrophy, prostatic carcinoma, pancreatic carcinoma, and spinal cord injury, alone or combination with other therapies.
- the molecules of the present invention will be useful for proliferation of neuronal, prostatic and pancreatic tissue cells, such as pancreatic islets, pancreatic acinar cells, neuroectoderm, neurons of the central nervous systems, and sympathetic neurons in vitro.
- Molecules of the present invention will be useful for growth and differentiation of hematopoietic cells directly or by the means of stimulating stromal cells that support hematopoietic cells proliferation and differentiation.
- molecules of the present invention are useful as components of defined cell culture media, and may be used alone or in combination with other cytokines and hormones to replace serum that is commonly used in cell culture.
- Molecules of the present invention are particularly useful in specifically promoting the growth and/or development of pancreatic islets, prostate cells (e.g., PZ-HPV-7 human prostate epithelium cells ATCC Number: CRL-2221 and rat YPEN-1 normal prostate cells, ATCC Number: CRL- 2222); neuronal cells (e.g., mouse CATH.a brain neuronal cells ATCC Number: CRL- 1 1179, human HCN-1A neuronal cells, ATCC Number: CRL- 10442) in culture, and may also prove useful in the study of hype ⁇ lasia and regeneration.
- Other types of cells for which zFGF12 molecules will be useful for establishing and maintaining cell cultures include epithelial cells and keratinocytes. Epithelial cells can be isolated from, for example, prostate, cornea, lung, mammary or kidney tissues.
- polypeptides, nucleic acid and/or antibodies of the present invention may be used in treatment of disorders associated with diabetes mellitus, neural cell development or degeneration, amyotrophic lateral sclerosis, cerebrovascular stroke, neurophathy associated with lack of maintenance of neuronal differentiation, and congenital disorders of the nervous system or lack of neuronal development.
- Molecules of the present invention may also be useful for promoting angiogenesis and wound healing, for revascularization in the eye, for complications related to poor circulation such as diabetic foot ulcers, for stroke, following coronary reperfusion using pharmacologic methods and other indications where angiogenesis is of benefit, such as vascular diseases of the extremities.
- Molecules of the present invention may be useful for improving cardiac function, either by inducing cardiac myocyte neogenesis and/or hype ⁇ lasia, by inducing coronary collateral formation, or by inducing remodeling of necrotic myocardial area.
- ZFGF12 will be useful for promoting wound healing of the epidermis.
- the molecules of the present invention can be used to protect and promote recovery of the epithelial cells in the gastrointestinal tract, small intestine and oral muscosa after treat with chemotherapy and/or radiation. Stimulation of lung epithelial cells lining the air space can promote recovery from lung injury and complications associated with premature birth in neonates. ZFGF12 may also modulate surfactant production in the lung epithelium. Other epithelial cells are found in prostate, cornea, mammary and kidney tissue, and the proliferation and specialized cell functions of these cells can be modulated by zFGF12.
- An ischemic event is the disruption of blood flow to an organ, resulting in necrosis or infarct of the non-perfused region.
- Ischemia-reperfusion is the interruption of blood flow to an organ, such as the heart or brain, and subsequent restoration (often abrupt) of blood flow. While restoration of blood flow is essential to preserve functional tissue, the reperfusion itself is known to be deleterious. In fact, there is evidence that reperfusion of an ischemic area compromises endothelium- dependent vessel relaxation resulting in vasospasms, and in the heart compromised coronary vasodilation, that is not seen in an ischemic event without reperfusion (Cuevas et al., Growth Factors L5:29-40, 1997).
- ischemia and reperfusion are important contributors to tissue necrosis, such as a myocardial infarct or stroke.
- the molecules of the present invention will have therapeutic value to reduce damage to the tissues caused by ischemia or ischemia-reperfusion events, particularly in the heart or brain.
- Other therapeutic uses for the present invention include induction of skeletal muscle neogenesis and/or hype ⁇ lasia, kidney regeneration and/or for treatment of systemic and pulmonary hypertension.
- ZFGF12 induced coronary collateral development is measured in rabbits, dogs or pigs using models of chronic coronary occlusion (Landau et al., Amer. Heart J. 29:924-931, 1995; Sellke et al., Surgery 120(2): 182-188, 1996 and Lazarous et al., 1996, ibid.)
- zFGF12 benefits for treating stroke is tested in vivo in rats utilizing bilateral carotid artery occlusion and measuring histological changes, as well as maze performance (Gage et al., Neurobiol. Aging 9:645-655, 1988).
- Molecules of the present invention can be used to target the delivery of agents or drugs to the cells and/or tissues derived from the neuroectoderm, the developing central nervous systems, the developing peripheral nervous system, the developing spinal cord, prostate and pancreas.
- the molecules of the present invention will be useful limiting expression to the neural tissue, by virtue of the tissue specific expression directed by the zFGF12 promoter.
- neural tissue-specific expression can be achieved using a zFGF12-adenoviral discistronic construct (Rothmann et al., Gene Therapy 3:919-926, 1996).
- the zFGF12 polypeptides can be used to restrict other agents or drugs to neural tissue by linking zFGF12 polypeptides to another protein (Franz et al., Circ. Res. 73:629-638, 1993) by linking a first molecule that is comprised of a zFGF12 homolog polypeptide with a second agent or drug to form a chimera.
- Proteins, for instance antibodies, can be used to form chimeras with zFGF12 molecules of the present invention (Narula et al., J. Nucl. Cardiol. 2:26-34, 1995).
- agents or drugs include, but are not limited to, bioactive-polypeptides, genes, toxins, radionuclides, small molecule pharmaceuticals and the like.
- Linking may be direct or indirect (e.g., liposomes), and may occur by recombinant means, chemical linkage, strong non-covalent interaction and the like.
- the proteins of the present invention are formulated for parenteral, particularly intravenous or subcutaneous, administration according to conventional methods.
- Intravenous administration will be by bolus injection or infusion over a typical period of one to several hours.
- pharmaceutical formulations will include a zFGF12 protein in combination with a pharmaceutically acceptable vehicle, such as saline, buffered saline, 5% dextrose in water or the like.
- Formulations may further include one or more excipients, preservatives, solubilizers, buffering agents, albumin to prevent protein loss on vial surfaces, etc.
- Therapeutic doses will generally be in the range of 0.1 to 100 ⁇ g/kg of patient weight per day, preferably 0.5- 20 ⁇ g/kg per day, with the exact dose determined by the clinician according to accepted standards, taking into account the nature and severity of the condition to be treated, patient traits, etc. Determination of dose is within the level of ordinary skill in the art.
- the proteins may be administered for acute treatment, over one week or less, often over a period of one to three days or may be used in chronic treatment, over several months or years.
- a therapeutically effective amount of zFGF12 is an amount sufficient to produce a clinically significant change in proliferation, or increases in specific cell types associated with mesenchymal stem cells and progenitors.
- ZFGF12 polypeptides can also be used to teach analytical skills such as mass spectrometry, circular dichroism, to determine conformation, especially of the four alpha helices, x-ray crystallography to determine the three-dimensional structure in atomic detail, nuclear magnetic resonance spectroscopy to reveal the structure of proteins in solution.
- analytical skills such as mass spectrometry, circular dichroism, to determine conformation, especially of the four alpha helices, x-ray crystallography to determine the three-dimensional structure in atomic detail, nuclear magnetic resonance spectroscopy to reveal the structure of proteins in solution.
- a kit containing the ZFGF12 can be given to the student to analyze. Since the amino acid sequence would be known by the instructor, the protein can be given to the student as a test to determine the skills or develop the skills of the student, the instructor would then know whether or not the student has correctly analyzed the polypeptide Since every polypeptide is unique, the educational utility of ZFGF12 would be unique unto itself
- the antibodies which bind specifically to ZFGF12 can be used as a teaching aid to instruct students how to prepare affinity chromatography columns to purify ZFGF12, cloning and sequencing the polynucleotide that encodes an antibody and thus as a practicum for teaching a student how to design humanized antibodies
- the ZFGF12 gene, polypeptide, or antibody would then be packaged by reagent companies and sold to educational institutions so that the students gain skill in art of molecular biology Because each gene and protein is unique, each gene and protein creates unique challenges and learning experiences for students in a lab practicum
- Such educational kits containing the ZFGF12 gene, polypeptide, or antibody are considered within the scope of the present invention.
- the present invention provides isolated polypeptides comprising a sequence of amino acid residues that is at least 95% identical to the sequence as shown in SEQ ID NO 2 from residue 25 to residue 251
- the polypeptides of the present invention will be 95% identical with a cysteine at position 1 13, a phenylahne at position 1 15, and a histidine at position 1 17
- the polypeptides with further comprise a leucine at positions 53, 73, and 102, a valine at positions 61 , 83, 94, and 136, an isoleucine at positions 75 and 85, a cysteine at position 113, a phenylalanine at position 115, a tyrosine at position 127, as shown in SEQ ID NO 2
- the present invention also provides polypeptides that comprise the sequence of SEQ ID NO 2 as shown trom amino acid residue 25 to amino acid residue 251, and as shown from amino acid residue 1 to amino acid residue 251
- the present invention provides for expression vectors that comp ⁇ se a transcriptional promoter, a DNA segment encoding the polypeptides described herein, and a transcriptional terminator
- the present invention includes cultured cells expressing the polypeptides desc ⁇ bed herein, as well as methods for making and recovering those polypeptides and the proteins that comprise those polypeptides
- the present invention provides antibodies that specifically bind the polypeptides described herein and proteins that comprise those polypeptides
- the present invention provides polynucleotides comprising nucleotide sequences that encode for the polypeptides described herein, and those shown in SEQ ID NO: 1 from nucleotide 187 to nucleotide 870 and shown in SEQ ID NO: 3 from nucleotide 72 to nucleotide 753.
- the present invention will provide for fusion proteins that comprise at two polypeptides, of which at least one of those polypeptides will comprise a sequence of amino acid residues as shown in SEQ ID NO: 2 from residue 25 to residue 251 , or some other polypeptide described herein a molecule of the present invention.
- the present invention provides for methods of using the molecules of the present invention.
- one method is use as a factor to stimulate the growth and/or differentiation of mesenchymal lineage cells comprising culturing mesenchymal stem cells or progenitor cells in the presence of zFGF12 polypeptides described herein in an amount sufficient to increase the number of mesenchymal cells as compared to cells grown in the absence of zFGF12 polypeptides.
- Homologous recombination in yeast is used to create expression plasmids containing the polynucleotide encoding zFGF12 for expression in mammalian cells.
- To construct the zFGF12/pCZF199 expression vectors the following DNA fragments are transformed into S. cerevisiae: Sna BI digested pCZR199 as an acceptor vector, the zFGF12 EcoRI restriction fragment, and two, double stranded linker segments.
- the expression vector, pCZR199 has yeast replication elements, (CEN, ARS), the selectable marker, URA3, E.
- coli replication elements e.g., AMP ⁇ and ori
- a blunt-ended cloning site Sna BI
- adds either a N-terminal or C-terminal Glu-Glu tag SEQ ID NO: 6
- the vectors are used to create zFGF12 polypeptides having either end of the expressed protein Glu-Glu tagged.
- the double stranded linker segments are prepared using PCR.
- the linkers served to join the vector to the insert fragments at both the 5' and 3' ends. Two sets of linkers are prepared.
- One set of linkers joins the insert to a vector placing the Glu-Glu tag (SEQ ID NO: 6) on the 5' end of the insert sequence using a linker.
- the second set of linkers is used to join the zFGF12 insert into a vector placing a 3' Glu-Glu tag (SEQ ID NO: 6).
- a third set of linkers is used to join the zFGF12 insert into the vector, resulting in an untagged constructs
- the 5' linker is same as the linked used for the C-terminally Glu-Glu tagged zFGF12.
- the 3' linker is the same as the linker used for the N-terminally Glu-Glu tagged zFGF12.
- the oligonucleotides are joined using standard PCR reaction conditions and heated to 94°C for 1.5 minutes followed by 10 cycles at 94°C for 30 seconds, 50°C for 1 minute and 72°C for 1 minute, then a 10 minute extension at 72°C.
- the DNA fragments are added to 100 ?1 competent yeast (Genetic strain SF838-9D , Roffman et al., EMBO J. 8:2057-65, 1989) and electroporated.
- the yeast cells are immediately diluted in 600 ?1 1.2 M sorbitol and plated on Ura D plates and incubated at 30oC for 48 hours.
- Ura + colonies are selected from both the N-terminally- tagged and C-terminally-tagged zFGF12 proteins and the DNA from the resulting yeast colonies is extracted and transformed into E. coli. Individual clones harboring the correct expression construct are identified by restriction digests. DNA sequencing confirms that the desired sequences has been enjoined with one another.
- Large scale plasmid DNA is isolated from one or more correct clones from both the N- and C-terminally tagged zFGF12 sequences, the expression cassette liberated from the vector and transformed into yeast or E. coli for large scale protein production.
- the concentrated material is again sterile-filtered with the Gelman filter as described above.
- a mixture of protease inhibitors is added to the concentrated conditioned media to final concentrations of 2.5 mM ethylenediaminetetraacetic acid (EDTA, Sigma Chemical Co. St. Louis, MO), 0.001 mM leupeptin (Boehringer-Mannheim, Indianapolis, IN), 0.001 mM pepstatin (Boehringer-Mannheim) and 0.4 mM Pefabloc (Boehringer-Mannheim).
- the concentrated conditioned media is applied to a 5.0 x 15.0 cm AF
- Heparin 650m (TosoHaas, Montgomeryville, PA) column equilibrated in 0.25M NaCI, 50 mM sodium phosphate, pH 7.2 at a flow rate of 5 ml/min using a BioCad Sprint
- HPLC PerSeptive BioSystems, Framingham, MA. Two-ml fractions are collected and the absorbance at 280 nM is monitored. After sample application, the column is washed with 10 column volumes of loading buffer and when the absorbance of the effluent is less than that 0.05, the column is eluted with a three column volume gradient from 0.25 M to 2.0 M NaCI in 50 mM sodium phosphate, pH 7.2. The fractions containing zFGF12 are identified by SDS-PAGE and western blotting with anti-zFGF12 antibodies.
- Fractions containing zFGF12 are pooled together and diluted ten-fold into 50 mM sodium phosphate pH 7.5 and the material is applied to a 1.5 x 20.0 cm Poros HS cation exchange column equilibrated in 50 mM phosphate pH 7.5 using the BioCad Sprint as described above. After sample application, the column is washed with 10 column volumes of loading buffer and when the absorbance of the effluent is less than that 0.05, the column is eluted with a 40.0 column volume gradient from 0.0 M to 2.0 M NaCI in 50 mM sodium phosphate, pH 7.5. Fractions are collected as described above and those containing zFGF12 will be identified by SDS-PAGE and Western blotting, pooled together and concentrated using an Amicon stirred cell fitted with a YM-10 membrane.
- the concentrated material is then be applied to a 3.5 x 100 cm Sephacryl-SlOO gel filtration column equilibrated in 1.0 M NaCI, 0.01 M EDTA and 0.05 M sodium phosphate, pH 7.2. Fractions are analyzed by SDS-PAGE and Western blotting with anti-zFGF12 antibodies as described above. Fractions containing pure zFGF12 are pooled together and samples are taken for amino acid analysis and N- terminal sequencing. The remainder of the sample is aliquoted, and stored at -80°C.
- E.coli fermentation medium is obtained from a strain expressing zFGF12 as a Maltose Binding protein fusion.
- the MBPzFGF12 fusion is solubilized during sonication or French press rupture, using a buffer containing 20 mM Hepes, 0.4 M Nacl, 0.01 M EDTA, 10 mM DTT, at pH 7.4.
- the extraction buffer also includes 5 ⁇ g/ml quantities of Pepstatin, Leupeptin, Aprotinin, Bestatin. Phenyl methyl sulfonylfluoride (PMSF) is also included at a final concentration of 0.5 mM.
- PMSF Phenyl methyl sulfonylfluoride
- the extract is spun at 18,000 x g for 30 minutes at 4°C.
- the resulting supernatent is processed on an Amylose resin (Pharmacia LKB Biotechnology,
- MBPzFGF12 fusion binds the MBP domain of the fusion.
- the bound MBPzFGF12 fusion is eluted in the same buffer as extraction buffer without DTT and protease inhibitors but containing 10 mM Maltose.
- the eluted pool of MBPzFGF12 is treated with 1:100 (w/w) Bovine thrombin to MBPzFGF12 fusion.
- the cleavage reaction is allowed to proceed for 6 to 8 hours at room temperature, after which the reaction mixture is passed over a bed of Benzamidine sepharose (Pharmacia LKB Biotechnology, Piscataway, NJ) to remove the thrombin, using the same elution buffer as described above for Amylose affinity chromatography.
- the passed fraction, containing the cleaved product zFGF12 and free MBP domain are applied to a Toso Haas Heparin affinity matrix (Toso Haas, Montgomeryville, PA) equilibrated in 0.5 M NaCI, 20 mM Hepes, 0.01 M EDTA at pH 7.4.
- the MBP and zFGF 12 both bound to heparin under these conditions.
- the bound proteins are eluted with a 2 to 3 column volume gradient formed between 0.5M NaCI and 2.0 M NaCI in column buffer.
- Example 4 For construction of adenovirus vectors, the protein coding region of human zFGF12 is amplified by PCR using primers that add Pmel and Ascl restriction sites at the 5' and 3' termini respectively. Amplification is performed with a full-length zFGF12 cDNA template in a PCR reaction as follows: one cycle at 95°C for 5 minutes; followed by 15 cycles at 95°C for 1 min., 61°C for 1 min., and 72°C for 1.5 min.; followed by 72°C for 7 min.; followed by a 4°C soak. The PCR reaction product is loaded onto a 1.2% low-melting-temperature agarose gel in TAE buffer (0.04 M Tris- acetate, 0.001 M EDTA).
- the zFGF12 PCR product is excised from the gel and purified using a commercially available kit comprising a silica gel mambrane spin column (QIAquick® PCR Purification Kit and gel cleanup kit; Qiagen, Inc.) as per kit instructions.
- the PCR product is then digested with Pmel and Ascl, phenol/chloroform extracted, EtOH precipitated, and rehydrated in 20 ml TE (Tris/EDTA pH 8).
- the zFGF12 fragment is then ligated into the Pmel-Ascl sites of the transgenic vector pTG12-8 and transformed into E. coli DH10BTM competent cells by electroporation.
- Vector pTG12-8 was derived from p2999B4 (Palmiter et al., Mol. Cell Biol. L3:5266- 5275, 1993) by insertion of a rat insulin II intron (ca. 200 bp) and polylinker (Fse I/Pme
- the vector comprises a mouse metallothionein (MT-1) promoter (ca. 750 bp) and human growth hormone (hGH) untranslated region and polyadenylation signal (ca. 650 bp) flanked by 10 kb of MT-1 5' flanking sequence and 7 kb of MT-1 3' flanking sequence.
- MT-1 mouse metallothionein
- hGH human growth hormone
- polyadenylation signal ca. 650 bp
- DNA is prepared using a commercially available kit (Maxi Kit, Qiagen, Inc.), and the zFGF12 cDNA is released from the pTG12-8 vector using Pmel and Ascl enzymes.
- the cDNA is isolated on a 1 % low melting temperature agarose gel and excised from the gel. The gel slice is melted at 70?C, and the DNA is extracted twice with an equal volume of Tris-buffered phenol, precipitated with EtOH, and resuspended in 10 ⁇ l H2O.
- the zFGF12 cDNA is cloned into the EcoRV-Asd sites of a modified pAdTrack-CMV (He, T-C. et al., Proc. Natl. Acad. Sci. USA 95:2509-2514, 1998).
- This construct contains the green fluorescent protein (GFP) marker gene.
- the CMV promoter driving GFP expression is replaced with the SV40 promoter, and the SV40 polyadenylation signal is replaced with the human growth hormone polyadenylation signal.
- the native polylinker is replaced with Fsel, EcoRV, and Ascl sites.
- This modified form of pAdTrack-CMV is named pZyTrack.
- Ligation is performed using a commercially available DNA ligation and screening kit (Fast-Link® kit; Epicentre Technologies, Madison, WI).
- Clones containing zFGF12 are identified by digestion of mini prep DNA with Fsel and Ascl.
- Fsel and Ascl In order to linearize the plasmid, approximately 5 ⁇ g of the resulting pZyTrack zFGF12 plasmid is digested with Pmel.
- Approximately 1 ⁇ g of the linearized plasmid is cotransformed with 200 ng of supercoiled pAdEasy (He et al., ibid.) into E. coli BJ5183 cells (He et al., ibid.).
- the co-transformation is done using a Bio-Rad Gene Pulser at 2.5 kV, 200 ohms and 25 ⁇ Fa.
- the entire co-transformation mixture is plated on 4 LB plates containing 25 ⁇ g/ml kanamycin. The smallest colonies are picked and expanded in LB/kanamycin, and recombinant adenovirus DNA is identified by standard DNA miniprep procedures.
- the recombinant adenovirus miniprep DNA is transformed into E. coli DH10BTM competent cells, and DNA is prepared using a Maxi Kit (Qiagen, Inc.) aaccording to kit instructions.
- recombinant adenoviral DNA is digested with Pa enzyme (New England Biolabs) for 3 hours at 37°C in a reaction volume of 100 ⁇ l containing 20-30U of Pad.
- the digested DNA is extracted twice with an equal volume of phenol/chloroform and precipitated with ethanol.
- the DNA pellet is resuspended in 10 ⁇ l distilled water.
- a T25 flask of QBI-293A cells Quantantum Biotechnologies, Inc. Montreal, Qc. Canada, inoculated the day before and grown to 60-70% confluence, is transfected with the Pad digested DNA.
- the Pad-digested DNA is diluted up to a total volume of 50 ⁇ l with sterile HBS (150mM NaCI, 20mM HEPES).
- HBS 150mM NaCI, 20mM HEPES
- 20 ⁇ l of 1 mg/ml N-[l-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethyl-ammonium salts (DOTAP) (Boehringer Mannheim, Indianapolis, IN) is diluted to a total volume of 100 ⁇ l with HBS.
- DOTAP N-[l-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethyl-ammonium salts
- the media is removed from the 293A cells and washed with 5 ml serum-free minimum essential medium (MEM) alpha containing ImM sodium pyruvate, 0.1 mM MEM non-essential amino acids, and 25mM HEPES buffer (reagents obtained from Life Technologies, Gaithersburg, MD).
- 5 ml of serum-free MEM is added to the 293A cells and held at 37°C.
- the DNA/lipid mixture is added drop-wise to the T25 flask of 293A cells, mixed gently, and incubated at 37°C for 4 hours. After 4 hours the media containing the DNA/lipid mixture is aspirated off and replaced with 5 ml complete MEM containing 5% fetal bovine serum.
- the transfected cells are monitored for GFP expression and formation of foci (viral plaques).
- the crude viral lysate is collected using a cell scraper to collect all of the 293A cells.
- the lysate is transferred to a 50-ml conical tube.
- three freeze/thaw cycles are done in a dry ice/ethanol bath and a 37°C waterbath.
- the crude lysate is amplified (Primary (1°) amplification) to obtain a working "stock" of zFGF12 rAdV lysate.
- a secondary (2°) amplification of zFGF12 rAdV is then performed. Twenty 15-cm tissue culture dishes of 293 A cells are prepared so that the cells are 80- 90% confluent. All but 20 ml of 5% MEM media is removed, and each dish is inoculated with 300-500 ml of the 1° amplified rAdv lysate. After 48 hours the 293 A cells are lysed from virus production, the lysate is collected into 250-ml polypropylene centrifuge bottles, and the rAdV is purified.
- NP-40 detergent is added to a final concentration of 0.5% to the bottles of crude lysate in order to lyse all cells.
- Bottles are placed on a rotating platform for 10 minutes agitating as fast as possible without the bottles falling over.
- the debris is pelleted by centrifugation at 20,000 X G for 15 minutes.
- the supernatant is transferred to 250-ml polycarbonate cer trifuge bottles, and 0.5 volume of 20% PEG8000/2.5 M NaCI solution is added.
- the bottles are shaken overnight on ice.
- the bottles are centrifuged at 20,000 X G for 15 minutes, and the supernatant is discarded into a bleach solution.
- the white, virus/PEG precipitate from 2 bottles is resuspended in 2.5 ml PBS.
- the resulting virus solution is placed in 2-ml microcentrifuge tubes and centrifuged at 14,000 X G in the microcentrifuge for 10 minutes to remove any additional cell debris.
- the supernatant from the 2-ml microcentrifuge tubes is transferred into a 15-ml polypropylene snapcap tube and adjusted to a density of 1.34 g/ml with CsCl.
- the solution is transferred to 3.2-ml, polycarbonate, thick-walled centrifuge tubes and spun at 348,000 X G for 3-4 hours at 25?C.
- the virus forms a white band. Using wide-bore pipette tips, the virus band is collected.
- a commercially available ion-exchange columns (e.g., PD- 10 columns prepacked with Sephadex® G-25M; Pharmacia Biotech, Piscataway, NJ) is used to desalt the virus preparation.
- the column is equilibrated with 20 ml of PBS.
- the virus is loaded and allowed to run into the column.
- 5 ml of PBS is added to the column, and fractions of 8-10 drops are collected.
- glycerol is added to the purified virus to a final concentration of 15%, mixed gently but effectively, and stored in aiiquots at -80°C.
- a panel of cDNAs from human tissues is screened for zFGF12 expression using PCR.
- the panel is made in-house and contained 94 marathon cDNA and cDNA samples from various normal and cancerous human tissues and cell lines is shown in Table 5, below.
- the cDNAs come from in-house libraries or marathon cDNAs from in-house RNA preps, Clontech RNA, or Invitrogen RNA.
- the marathon cDNAs are made using the marathon-ReadyTM kit (Clontech, Palo Alto, CA) and QC tested with clathnn primers, and then diluted based on the intensity of the clath ⁇ n band.
- RNA quality used for the libraries are tested lor average insert size by PCR with vector ohgos that are specific for the vector sequences for an individual cDNA library, (2) Standardization of the concentration of the cDNA in panel samples is achieved using standard PCR methods to amplify full length alpha tubuhn or G3PDH cDNA using a 5' vector ohgonucleotide and 3' alpha tubuhn specific ohgonucleotide primer or 3' G3PDH specific oligo primer, and (3) a sample is sequenced to check for possible ⁇ bosomal or mitochond ⁇ al DNA contamination.
- the panel is set up in a 96-well format that included a human genomic DNA (Clontech, Palo Alto, CA) positive control sample. Each well contains approximately 0 2-100 pg/ ⁇ l of cDNA
- the PCR reactions are set up using appropriate oligonucleotides, TaKaRa Ex TaqTM (TAKARA Shuzo Co LTD, Biomedicals Group, Japan), and Rediload dye (Research Genetics, Inc., Huntsville, AL)
- the typical amplification is carried out as follows: 1 cycle at 94°C for 2 minutes, 35 cycles of 94°C for 30 seconds, 66.3°C for 30 seconds and 72°C for 30 seconds, followed by 1 cycle at 72°C for 5 minutes.
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Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
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EP01901722A EP1246843A1 (en) | 2000-01-05 | 2001-01-04 | Novel fgf homolog zfgf12 |
AU27592/01A AU2759201A (en) | 2000-01-05 | 2001-01-04 | Novel fgf homolog zfgf12 |
JP2001550280A JP2003518944A (en) | 2000-01-05 | 2001-01-04 | Novel FGF homolog ZFGF12 |
CA002396401A CA2396401A1 (en) | 2000-01-05 | 2001-01-04 | Novel fgf homolog zfgf12 |
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US47806200A | 2000-01-05 | 2000-01-05 | |
US09/478,062 | 2000-01-05 |
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WO2001049740A1 true WO2001049740A1 (en) | 2001-07-12 |
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PCT/US2001/000238 WO2001049740A1 (en) | 2000-01-05 | 2001-01-04 | Novel fgf homolog zfgf12 |
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JP (1) | JP2003518944A (en) |
AU (1) | AU2759201A (en) |
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Also Published As
Publication number | Publication date |
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AU2759201A (en) | 2001-07-16 |
JP2003518944A (en) | 2003-06-17 |
CA2396401A1 (en) | 2001-07-12 |
EP1246843A1 (en) | 2002-10-09 |
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