WO2001035317A1 - Determining the functions and interactions of proteins by comparative analysis - Google Patents

Abstract

The invention provides novel methods for characterizing the function of nucleic acids and polypeptides. The invention provides a novel method for identifying a nucleic acid or a polypeptide sequence that may be a target for a drug. The invention provides a novel method for identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism. The characterization is based on use of methods of the invention comprising algorithms that can identify functional relationships between diverse sets of non-homologous nucleic acid and polypeptide sequences. The invention provides a computer program product, stored on a computer-readable medium, for identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism. The invention provides a computer program product, stored on a computer-readable medium, for identifying a nucleic acid or a polypeptide sequence that may be a target for a drug. The invention provides a computer system, comprising a processor and a computer program product of the invention.

Description

DETERMINING THE FUNCTIONS AND INTERACTIONS OF PROTEINS BY COMPARATIVE ANALYSIS

Related Applications

The present application is a continuation-in-part application ("CIP") of Patent Convention Treaty (PCT) International Application Serial No: PCT/USO0/02246, filed in the U.S. receiving office on January 28, 2000, and this application claims the benefit of priority under 35 U.S.C. § 119(e) of U.S. Provisional Application Nos. 60/165,124, and 60/165,086, both filed November 12, 1999, and U.S. Provisional Application No. 60/179,531, filed February 1, 2000. International Application Serial No: PCT USOO/02246 claims the benefit of priority under 35 U.S.C. § 119(e) of U.S. Provisional Application Serial No. 60/117,844, filed January 29, 1999, U.S. Provisional Application Serial No. 60/118,206, filed February 1, 1999, U.S. Provisional Application Serial No. 60/126,593, filed March 26, 1999, U.S. Provisional Applications Serial No. 60/134,093, filed May 14, 1999, and U.S. Provisional Application Serial No. 60/134,092, filed May 14, 1999. Each of the aforementioned applications is explicitly incoφorated herein by reference in their entirety and for all puφoses.

TECHNICAL FIELD

This invention generally relates to genetics and microbiology. The invention provides novel methods to identify the function of and relationships between nucleic acid and protein sequences. The method is particularly useful for finding the identifying genes and polypeptides having potential therapeutic relevance in organisms, e.g., microorganisms, such as Mycobacterium tuberculosis. The invention also provides Mycobacterium tuberculosis genes and polypeptides found by these methods. These genes and polypeptides are useful as potential drug targets.

BACKGROUND The determination of the functions of and relationships between nucleic acid and protein sequences has traditionally relied on either the study of homology and sequence identity with genes and proteins of known function or, in the absence of informative homology, laborious experimental work. The availability of many complete genome sequences has made it possible to develop new strategies for computational determination of protein functions. Several methods have been developed which can predict the general function of proteins by analyzing their functional relationships rather than sequence similarity. Generally, two proteins can be considered functionally related when they form part of the same biochemical pathway or biological process. For example, although malate dehydrogenase is not homologous to pyruvate carboxylase, and the two enzymes do not catalyze the same reaction, they are functionally related because they both catalyze steps of a common biochemical pathway, namely the tricarboxylic acid cycle.

New methods that can establish such functional relationships could provide valuable information on the functions of uncharacterized nucleic acid and protein sequences. The disease tuberculosis, caused Mycobacterium tuberculosis (MTB) is one of the world's leading killers. The World Health Organization estimates that 30 million deaths from pulmonary tuberculosis will occur during this decade. Alarming reports on the emergence of drug-resistant strains of this bacterium underscore the importance of the search for new therapeutic agents. Identifying the function of every protein produced by MTB will provide researchers with promising new targets for anti-tuberculosis drag design.

SUMMARY

The invention provides novel methods for characterizing the function of nucleic acids and polypeptides. The invention provides a novel method for identifying a nucleic acid or a polypeptide sequence that may be a target for a drag. The invention provides a novel method for identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism. The characterization is based on use of methods of the invention comprising algorithms that can identify functional relationships between diverse sets of non-homologous nucleic acid and polypeptide sequences. Characterization of nucleic acid and protein sequences can be the basis for the development of compositions that can interact with those nucleic acids and polypeptides. For example, such characterization can provide a basis for screening methods. Such characterization may allow use of these sequences as targets for drug discovery. Discovery of such compositions can provide the basis for the design of novel drags, particularly if the characterized sequences are derived from a pathogen.

The invention provides a method for identifying a nucleic acid or a polypeptide sequence that may be a target for a drag comprising the following steps: (a) providing a first nucleic acid or a polypeptide sequence that is known to be a drag target; (b) providing at least one algorithm selected from the group consisting of a "domain fusion" method, a "phylogenetic profile" method and a "physiologic linkage" method, wherein the algorithm is capable analyzing a functional relationship between nucleic acid or polypeptide sequences; and, (c) comparing the first nucleic acid or the polypeptide drug target sequence to a plurality of sequences using at least one of the algorithms as set forth in step (b) to identify a second sequence that has a functional relationship to the first sequence, thereby identifying a nucleic acid or a polypeptide sequence that may be a target for a drug. The invention provides a method for identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism comprising the following steps: (a) providing a first nucleic acid or a polypeptide sequence that is known to be essential for the growth or viability of an organism; (b) providing at least one algorithm capable analyzing a functional relationship between nucleic acid or polypeptide sequences selected from the group consisting of a "domain fusion" method, a "phylogenetic profile" method and a "physiologic linkage" method; and, (c) comparing the first nucleic acid or the polypeptide sequence to a plurality of sequences using at least one of the algorithms as set forth in step (b) to identify a second sequence that has a functional relationship to the first sequence, thereby identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism. In one aspect of the methods of the invention, the drag is an anti-microbial drag. In another aspect, the first nucleic acid or a polypeptide sequence is derived from a pathogen. The pathogen can be a microorganism, such as Mycobacterium tuberculosis (MTB).

The plurality of sequences used to identify a second sequence can comprise a database of the gene sequences of an entire genome of an organism. The plurality of sequences used to identify a second sequence can comprise a database of the gene sequences derived from a pathogen.

In one aspect of the methods of the invention, the "phylogenetic profile" method algorithm comprises (a) obtaining data, comprising a list of proteins from at least two genomes; (b) comparing the list of proteins to form a protein phylogenetic profile for each protein, wherein the protein phylogenetic profile indicates the presence or absence of a protein belonging to a particular protein family in each of the at least two genomes based on homology of the proteins; and (c) grouping the list of proteins based on similar profiles, wherein proteins with similar profiles are indicated to have a functional relationship. The phylogenetic profile can be in the form of a vector, matrix or phylogenetic tree. The "phylogenetic profile" method can further comprise determining the significance of homology between the proteins by computing a probability (p) value threshold. The probability can be set with respect to the value 1/NM, based on the total number of sequence comparisons that are to be performed, wherein N is the number of proteins in the first organism's genome and Min all other genomes. The presence or absence of a protein belonging to a particular protein family in each of the at least two genomes can be determined by calculating an evolutionary distance. The evolutionary distance can be calculated by: (a) aligning two sequences from the list of proteins; (b) determining an evolution probability process by constructing a conditional probability matrix: p(aa-→aa'), where aa and aa' are any amino acids, said conditional probability matrix being constructed by converting an amino acid substitution matrix from a log odds matrix to said conditional probability matrix; (c) accounting for an observed alignment of the constructed conditional probability matrix by taking the product of the conditional probabilities for each aligned pair during the alignment of the two sequences, represented by

; and, (d) n determining an evolutionary distance α from powers equation p'=p^α(aa→aa'), maximizing for P. The conditional probability matrix can be defined by a Markov process with substitution rates, over a fixed time interval. The conversion from an amino acid substitution matrix to a conditional probability matrix can be represented by:

BLOSUM62._J PB(Ϊ →j) =p(j)2 _j >

where BLOSUM62 is an amino acid substitution matrix, and P(i->j) is the probability that amino acid is replaced by amino acid j through point mutations according to BLOSUM62 scores. In one aspect, the 's are the abundances of amino acid j and are computed by solving a plurality of linear equations given by the normalization condition that:

∑Ps(i → j) = X . In alternative aspects of the methods of the invention, the "physiologic linkage" method algorithm identifies proteins and nucleic acids that participate in a common functional pathway; identifies proteins and nucleic acids that participate in the synthesis of a common structural complex; and, identifies proteins and nucleic acids that participate in a common metabolic pathway.

In one aspect of the invention, the "domain fusion" method algorithm comprises (a) aligning a first primary amino acid sequence of multiple distinct non- homologous polypeptides to second primary amino acid sequence of a plurality of proteins; and, (b) for any alignment found between the first primary amino acid sequences of all of such multiple distinct non-homologous polypeptides and at least one protein of the second primary amino acid sequences, outputting an indication identifying the aligned second primary amino acid sequence as an indication of a functional link between the aligned first and second polypeptide sequences. The aligning can be performed by an algorithm selected from the group consisting of a Smith- Waterman algorithm, Needleman-Wunsch algorithm, a BLAST algorithm, a FASTA algorithm, and a PSI-BLAST algorithm. The multiple distinct non-homologous polypeptides can be obtained by translating a nucleic acid sequence from a genome database. The plurality of proteins can have a known function. At least one of the multiple distinct non-homologous polypeptides can have a known function. At least one of the multiple distinct non-homologous polypeptides can have an unknown function. The alignment can be based on the degree of homology of the multiple distinct non-homologous polypeptides to the plurality of proteins. The "domain fusion" method can comprise determining the significance of the aligned and identified second primary amino acid sequence by computing a probability (p) value threshold. The probability threshold can be set with respect to the value 1/NM, based on the total number of sequence comparisons that are to be performed, wherein N is the number of proteins in a first organism's genome and M in all other genomes. The "domain fusion" method can further comprising filtering excessive functional links between one first primary amino acid sequence of multiple distinct non- homologous polypeptides and an excessive number of other distinct non-homologous polypeptides for any alignment found between the first primary amino acid sequences of the distinct non-homologous polypeptides and at least one of the second primary amino acid sequences of the plurality of proteins.

The invention provides a computer program product, stored on a computer- readable medium, for identifying a nucleic acid or a polypeptide sequence that may be a target for a drug, the computer program product comprising instructions for causing a computer system to be capable of: (a) inputting a first nucleic acid or a polypeptide sequence that is known to be a drag target; (b) accessing at least one algorithm capable analyzing a functional relationship between nucleic acid or polypeptide sequences selected from the group consisting of a "domain fusion" method, a "phylogenetic profile" method and a "physiologic linkage" method; and (c) comparing the first nucleic acid or the polypeptide drag target sequence to a plurality of sequences using at least one of the algorithms set forth in step (b) to identify a second sequence that has a functional relationship to the first sequence and generating an output identifying a nucleic acid or a polypeptide sequence that may be a target for a drug . The invention provides a computer program product, stored on a computer- readable medium, for identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism, the computer program product comprising instructions for causing a computer system to be capable of: (a) providing a first nucleic acid or a polypeptide sequence that is known to be essential for the growth or viability of an organism; (b) accessing at least one algorithm capable analyzing a functional relationship between nucleic acid or polypeptide sequences selected from the group consisting of a "domain fusion" method, a "phylogenetic profile" method and a "physiologic linkage" method; and, (c) comparing the first nucleic acid or the polypeptide sequence to a plurality of sequences using at least one of the algorithms set forth in step (b) to identify a second sequence that has a functional relationship to the first sequence and generating an output identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism.

The invention provides a computer system, comprising: (a) a processor; and, a computer program product of the invention. All publications, patents, patent applications, GenBank sequences and ATCC deposits, cited herein are hereby expressly incoφorated by reference for all puφoses.

The details of one or more embodiments of the invention are set forth in the accompanying drawings and the description below. Other features, objects, and advantages of the invention will be apparent from the description and drawings, and from the claims.

DESCRIPTION OF DRAWINGS

Figure 1 is an example of functional linkages predicted between InhA (Rv 1484) and other TB genes.

Figure 2 is an example of predicted functional linkages between embB (Rv 3795), which is a target of the drug ethambutol, and other TB genes using the phylogenetic profile method.

Figure 3 is an example of predicted functional linkages between five TB genes having homology to penicillin binding proteins and other TB genes.

Figure shows that gcpE (Rv 2868C) is predicted to be functional linked to cell wall metabolism.

Figure 5 shows predicted functional linkages of htrA (Rv 1223C) with other TB genes.

Like reference symbols in the various drawings indicate like elements.

DETAILED DESCRIPTION

The present invention provides novel methods for identifying the relationships between and the function of nucleic acid and polypeptide sequences. The methods of the invention identify novel genes and polypeptides on the basis of their functional linkage to other proteins whose biological function or processes is known or infened by homology. The genes and polypeptides identified by the methods of the invention can be used in screening methods for the identification of compositions which, by binding or otherwise interacting with the gene or polypeptide, are capable of modifying the physiology and growth of an organism. The compositions identified by these screening methods are useful as drugs and pharmaceuticals. Thus, genes and polypeptides identified by the methods of the invention, including the genes and polypeptides identified herein, can be used as potential drug targets.

One aspect of the invention provides methods for identifying the function of genes and polypeptides from Mycobacterium tuberculosis (MTB or TB). Based on this new functional determination, these genes and polypeptides can be used to screen for compositions capable of modifying the physiology and growth of Mycobacterium tuberculosis (TB). Thus, genes and polypeptides identified by the methods of the invention, including the genes and polypeptides identified herein, can be used as targets in screening protocols and can be useful as potential drug targets. The function of the TB genes and polypeptides of the present invention were identified using the methods of the invention; i.e., they were identified on the basis of their functional linkage to other proteins whose biological function or processes were known by experiment or infened by homology. TB genes and polypeptides that are functionally linked to genes known to be involved in pathogenesis or organisms survival are potential drag targets. Genes or polypeptides associated with TB pathogenesis, survival or that are important or unique to TB biochemical pathways are potential drug targets. TB genes and polypeptides that have no homologues identified in humans are potential drag targets. The function of many of the TB genes and polypeptides identified is based on the genes or polypeptides with which they are functionally linked. TB genes whose function was identified using the methods of the invention are effectively targeted by a drug (i.e., they can act as bonafide drug targets) provides proof of principle that the invention's methods for identifying functionally linked genes can identify TB genes and polypeptides that are drug targets. Further confirmation that the genes identified by the methods of the invention include bonafide drag targets can be supported by the fact that genes already known to be targets for drags have been independently identified, or "re-discovered," by the invention's methods.

The novel TB genes described herein are identified as being functionally related or linked to other genes, including other TB genes, such as a known TB drug target (e.g., InhA polypeptide, which is a target of isoniazid). These functional linkages are established using mathematical algorithms. The assignment or inference of a function to TB genes and polypeptides based on their linkage or relatedness to other genes and polypeptides is described in U.S. provisional application serial no. 60/165,086. Potential TB drug targets are identified by several methods discussed herein and in further detail in U.S. provisional application serial no. 60/134,092. Through the use of these methods, TB genes and polypeptides have been identified as potential drug targets and are illustrated on Tables 1 and 2, and Figures 1 to 5. The nucleotide and amino acid sequences of these potential drug targets are illustrated on Tables 3 and 4, respectively (see below).

The phrase "functional link," "functionally related" and grammatical variations thereof, when used in reference to genes or polypeptides, means that the genes or polypeptides are predicted to be linked or related. A particular example of functionally related or linked proteins is where two proteins participate in a biochemical or metabolic pathway (e.g., malate dehydrogenase and fumarase, which are both present in the TCA cycle). Thus, although functionally linked or related proteins may not have sequence homology to each other, they are linked by virtue of their participation in the same biochemical pathway. Other examples of linked or related polypeptides are where two polypeptides are part of a protein complex, physically interact, or act upon each another.

The "domain fusion" or "Rosetta Stone" method searches protein sequences across all known genomes and identifies proteins that are separate in one organism but joined as intramolecular domains into one larger protein in another organism. Such proteins that are separate in some organisms but joined in others often carry out related or sequential functions and are therefore functionally linked.

The phylogenetic profile method compares protein sequences across all known genomes and analyzes the pattern of inheritance of each protein across the different organisms. Proteins that have similar patterns of inheritance, either acquired or lost as a part of a group of proteins through evolution, are functionally linked. The gene proximity method identifies genes that remain physically close or "clustered" throughout evolution and are therefore functionally linked.

A particular example of the identification of a potential TB drag target would be to identify a TB gene or polypeptide functionally linked to a known drag target. Anti-TB drugs include isoniazid, rifampicin, ethambutol, streptomycin, pyrazxinamide, and thiacetazone. For isoniazid, this drag is believed to act through enoyl-acyl reductase InhA, resulting in mycolic acid biosynthesis inhibition. Thus, TB genes or polypeptides functionally linked to enoyl-acyl reductase InhA are potential drug targets; see Figure 1, which shows an analysis of InhA, the target for isoniazid, the most widely used anti- tuberculosis drag, and functional linkages to a set of genes mostly known or hypothesized to be involved in cell wall-related processes and lipid and polyketide metabolism. Particular examples of the identification of several TB genes and polypeptides that are functionally related to the target of these anti-TB drugs is shown in Figures 1 to 5.

"Domain Fusion" or "Rosetta Stone" Method

The "domain fusion" or "Rosetta Stone" method compares protein sequences across known nucleic acid databases (e.g., known genomes) to identify genes and proteins that are separate entities in one organism but are joined into one larger multidomain protein in another organism. In such cases, the two separate proteins often carry out related or sequential functions or form part of a larger protein complex. Therefore, the general function of one component (e.g., one or more of the unknown proteins) can be infened from the known function of the other component. In addition, merely identifying links between proteins using the method described herein provides valuable information (e.g., usefulness as a target for an antibacterial drug), regardless of whether the function of one or more of the proteins used to form the link(s) is known. Because the two components do not have similar amino acid sequence the function of one could not be infened from the other on the basis of sequence similarity alone. The methods for identifying drug targets (e.g., TB drug targets) described herein (e.g., the "Rosetta Stone Method") are based on the idea that proteins that participate in a common structural complex, metabolic pathway, biological process or with closely related physiological functions, are functionally linked. In addition, these methods also are capable of identifying proteins that interact physically with one another. Functionally linked proteins in one organism can often be found fused into a single polypeptide chain in a different organism. Similarly, fused proteins in one organism can be found as individual proteins in other organisms. For example, in a first organism one might identify two unlinked proteins "A" and "B" with unknown function. In another organism, one may find a single protein "AB" with a part that resembles "A" and a part that resembles "B". Protein AB allows one to predict that "A" and "B" are functionally related. The functional activity of each distinct protein in the "Rosetta Stone" method need not be known prior to performing the method (i.e., the function of A, B, or AB need not be known). Using the "Rosetta Stone" method to compare and analyze several unknown protein sequences can provide information regarding relationships of each protein absent knowledge about the functional activity of the initially analyzed proteins themselves. For example, the information (i.e., the links) can provide information that the proteins are part of a common pathway, function in a related process or physically interact. Such information need not be based on the biological function of the individual proteins.

These methods can provide information regarding links between previously un-linked proteins that function, for example, in a concerted process. A marker, for example, for a particular disease state is identified by the presence or absence of a protein (e.g., Her2/neu in breast cancer detection). Links (i.e., information) identified by the method, which link proteins "B" and "C" to such a marker suggest that proteins "B" and "C" are related by function, physical interaction or part of a common biological pathway with the marker. Such information is useful in designing screening methods and identifying drug targets (e.g., TB drag targets), making diagnostics, and designing therapeutics.

In one approach, the "Rosetta Stone" method is performed by sequence comparison that searches for incomplete "triangle relationships" between, for example, three proteins, i.e., for two proteins A' and B' that are different from one another but similar in sequence to another protein AB. Completing the triangle relationship provides useful information regarding the proteins' biological function(s), functional interaction, pathway relationships or physical relationships with other proteins in the "triangle."

Either nucleotide sequences or amino acid sequences can be used in the methods for identifying functionally related or linked genes or polypeptides. Where a nucleic sequence is to be used it can be first translated from a nucleic acid sequence to amino acid sequence. Such translation may be performed in all frames if the coding sequence is not known. Programs that can translate a nucleic acid sequence are known in the art. In addition, for simplicity, the description of this method discusses the use of a "pair" of proteins in the determination of a "Rosetta Stone" protein, more than 2 may be used (e.g., 3, 4, 5, 10, 100 or more proteins). Accordingly, one can analyze chains of linked proteins, such as "A" linked by a Rosetta Stone protein to "B" linked by a Rosetta Stone protein to "C", etc. By this method, groups of functionally related proteins can be found and their function identified.

A method can start with identifying the primary amino acid sequence for a plurality of proteins whose functional relationship is to be determined (e.g., protein A' and protein B'). A number of source databases are available, as described above, that contain either a nucleic acid sequence and/or a deduced amino acid sequence for use with the first step. The plurality of sequences (the "probe sequences") are then used to search a sequence database, e.g., GenBank (NCBI, NLM, NIH), PFAM (a large collection of multiple sequence alignments and hidden Markov models covering many common protein domains; Washington University, St. Louis MO) or ProDom (a database based on recursive P SI- BLAST searches and designed as a tool to help analyze domain anangements of proteins and protein families, see, e.g., Coφet (1999) Nucleic Acids Res. 27:263-267), either simultaneously or individually. Every protein in the sequence database is examined for its ability to act as a "Rosetta Stone" protein (i.e., a single protein containing polypeptide sequences or domains from both protein A' and protein B'). A number of different methods of performing such sequence searches are known in the art. Such sequence alignment methods include, for example, BLAST (see, e.g., Altschul (1990) J. Mol. Biol. 215: 403- 410), BLITZ (MPsrch) (see, e.g., Brenner (1995) Trends Genet. 11:330-331; and infra), and FASTA (see, e.g., Pearson (1988) Proc. Natl. Acad. Sci. USA 85(8):2444-2448; and infra). The probe sequence can be any length (e.g., about 50 amino acid residues to about 1000 amino acid residues).

Probe sequences (e.g., polypeptide sequences or domains) found in a single protein (e.g., an "AB" multidomain protein) are defined as being "linked" by that protein. Where the probe sequences are used individually to search the sequence database, one can mask those segments having homology to the first probe sequence found in the proteins of the sequence database prior to searching with the subsequent probe sequence. In this way, one eliminates any potential overlapping sequences between the two or more probe sequences.

The linked proteins can then be further compared for similarity with one another by amino acid sequence comparison. Where the sequences are identical or have high homology, such a finding can be indicative of the formation of homo-dimers, -trimers, etc. Typically, "Rosetta Stone"-linked proteins are only kept when the linked proteins show no homology to one another (e.g., hetero-dimers, trimers, etc.).

In another method for identifying functional linkages, a potential fusion protein lacking any functional information that is suspected of having two or more domains (e.g. , a potential "Rosetta Stone" protein) may be used to search for related proteins. In this method, the primary amino acid of the fusion protein is determined and used as a probe sequence. This probe sequence is used to search a sequence database (e.g., GenBank, PFAM or ProDom). Every protein in the sequence database is examined for homology to the potential fusion protein (i.e., multiple proteins containing polypeptide sequences or domains from the potential fusion protein). A number of different methods of performing such sequence searches are known in the art, e.g., BLAST, BLITZ (Biocomputing Research Unit, University of Edinburgh, Scotland, the "MPsrch program" performs comparisons of protein sequences against the Swiss-Prot protein sequence database using the Smith and Waterman best local similarity algorithm), and FASTA. Probe sequences found in more than one protein (e.g., A' and B' proteins) are defined as being "linked" so long as at least one protein per domain containing that domain but not the other is also identified. In other words, at least one protein or domain of the plurality of proteins must also be found alone in the sequence database. This verifies that the protein or domain is not an integral part of a first protein but rather a second independent protein having its own functional characteristics.

Statistical methods can be used to judge the significance of possible matches. The statistical significance of an alignment score is described by the probability, P, of obtaining a higher score when the sequences are shuffled. One way to compute a P value threshold is to first consider the total number of sequence comparisons that are to be performed. For example, if there are N proteins in E. coli and Min all other genomes this number is N x M If a comparison of this number of random sequence would result in one pair to yield a P value of X I NM by chance this then is set as the threshold.

This method provides information regarding which proteins are functionally related (e.g., related biological functions common stractural complexes, metabolic pathways or biological process) a subset of which physically interact in an organism. Alignment Algorithms

To align sequences, a number of different procedures can be used that produce a good match between the conesponding residues in the sequences. Typically, the Smith- Waterman (Smith (1981) Adv. Appl. Math. 2:482) or Needleman-Wunsch algorithm (Needleman (1970) J. Mol. Biol. 48:443) algorithm, are used, however, other, faster procedures such as BLAST, FASTA, PSI-BLAST (a version of Blast for finding protein families), or others known in the art (see infra discussion), can be used.

Filtering Methods

The Rosetta Stone Method provides at least two pieces of information. First the method provides information regarding which proteins are functionally related. Second the method provides information regarding which proteins are physically related. Each of these two pieces of information has different sources of enor and prediction. The first type of enor is introduced by protein sequences that occur in many different proteins and paired with many other protein sequences. The second type of enor is introduced due to there often being multiple copies of similar proteins, called paralogs, in a single organism. In general, the "Rosetta Stone" method predicts functionally related proteins well, with no filtering of results required. However, it is possible to filter the enor associated with either the first or second type of information.

The invention recognizes that a few domains are linked to an excessive number of other domains by a "Rosetta Stone" protein. For example, 95% of the domains are linked to fewer than 25 other domains. However, some domains, e.g., the Src Homology 3 (SH3) domain or ATP -binding cassette (ABC domains), link to more than a hundred other domains. These links were filtered by removing all links generated involving these 5% of domains (i.e., the domains linked to more than 25 other domains). For example, in E. coli, without filtering, 3531 links were identified using the domain-based analysis, but after filtering only 749 links were identified. This method improved prediction of functionally related proteins by 28% and physically related proteins by 47%. Accordingly, there are a number of ways to filter the results to improve the significance of the functional links. As described above, as the number of functional links increases there is an increased higher chance of finding a "Rosetta Stone" protein. By reducing the excessively linked proteins one reduces the chance number of "Rosetta Stone" proteins thereby increasing the significance of a functional link.

Enor introduced by multiple paralogs of linked proteins should have little effect on functional prediction, as paralogs usually have very similar function, but will affect the reliability of prediction of protein-protein interactions. This estimate is calculated for each linked protein pair, and can be estimated roughly as:

Fractional Enor = 1 ■ N

N where N is the number of paralogous protein pairs, (e.g., A linked to B, A' linked to B', A linked to B', and A' linked to B, in the case that A and A' are paralogs, as are B and B', and the linking proteins is AB as above).

The enor can also be estimated as X-T, where T is the mean percent of potential true positives calculated for all domain pairs in an organism. For each domain pair linked by a Rosetta Stone protein, there are n proteins with the first domain but not the second, and m proteins with the second domain but not the first. The percent of true positives T is therefore estimated as the smaller of n or m divided by n times m. As this enor T can be calculated for each set of linked domains, it can describe the confidence in any particular predicted interaction. In addition, the enor in functional links can be caused by small conserved regions or repeated common amino acid sequences being repeatedly identified in a "Rosetta Stone" protein by a plurality of distinct non-homologous polypeptides. To reduce this enor the percent of identity between the "Rosetta Stone" and the distinct non-homologous polypeptide can be measured. Alignment percentages of about 50% to about 90%, or, alternatively, about 75%, between the "Rosetta Stone" and the distinct polypeptide are indicative of links that are not subject to the small peptide sequence.

Phylogenetic Pathway Method

The "phylogenetic profile" method compares protein sequences across all known genomes and analyzes the pattern of inheritance of each protein across the different organisms. In its simplest form, each protein is simply characterized by its presence or absence in each organism. For example, if there are 16 known genomes, then each protein may be assigned a 16-bit code or phylogenetic profile. Since proteins that function together (e.g. , in the same metabolic pathway or as part of a larger functional or structural complex) evolve in a conelated fashion, they should have the same or similar patterns of inheritance, and therefore similar phylogenetic profiles. Therefore, the function of one protein may be infened from the function of another protein, which has a similar profile, if its function is known. As with the Rosetta Stone method, the function of one protein is infened from the function of another protein which is dissimilar in sequence. Furthermore, the predicted link between the proteins has utility in developing, for example, drag targets, diagnostics and therapeutics.

The phylogenetic profile method can be implemented in a binary code (i.e., describing the presence or absence of a given protein in an organism) or a continuous code that describes how similar the related sequences are in the different genomes. In addition, grouping of similar protein profiles may be made wherein similar profiles are indicative of functionally related proteins. Furthermore, the requirements for similarity can be modified depending upon particular criteria by varying the difference in similar bit requirements. For example, criteria requiring that the degree of similarity in the profile include all 16 bits being identical can be set, but may be modified so that similarity in 15 bits of the 16 bits would indicate relatedness of the protein profiles as well. Statistical methods can be used to determine how similar two patterns must be in order to be related.

The phylogenetic profile method is applicable to any genome including, e.g., viral, bacterial, archaeal or eukaryotic. The method of phylogenetic profile grouping provides the prediction of function for a previously uncharacterized protein(s). The method also allows prediction of new functional roles for characterized proteins based upon functional linkages. It also provides potential informative connections (i.e., links) between uncharacterized proteins. To represent the subset of organisms that contain a homolog a phylogenetic profile is constructed for each protein. The simplest manner to represent a protein's phylogenetic history is via a binary phylogenetic profile for each protein. This profile is a string with N entries, each one bit, where N conesponds to the number of genomes. The number of genomes can be any number of two or more (e.g., 2, 3, 4, 5, 10, 100, to 1000 or more). The presence of a homolog to a given protein in the n^Λ genome is indicated with an entry of unity at the «^Λ position (e.g., in a binary system an entry of 1). If no homolog is found the entry is zero. Proteins are clustered according to the similarity of their phylogenetic profiles. Similar profiles show a conelated pattern of inheritance, and by implication, functional linkage. The method predicts that the functions of uncharacterized proteins are likely to be similar to characterized proteins within a cluster. In order to decide whether a genome contains a protein related to another particular protein, the query amino acid sequence is aligned with each of the proteins from the genome(s) in question using known alignment algorithm (see above). To determine the statistical significance of any alignment score, the probability, p, of obtaining a higher score when the sequences are shuffled is described. One way to compute ap value threshold is to first consider the total number of sequence comparisons that are being aligned. If there are N proteins in a first organism's genome and Min all other genomes this number is Nx M. If this number were compared to random sequences it would be expected that one pair would yield ap value of ¹ . This value can be set as a threshold. Other thresholds may be used

NM and will be recognized by those of skill in the art. A non-binary phylogenetic profile can be used. In this method, the phylogenetic profile is a string of N entries where the n^th entry represents the evolutionary distance of the query protein to the homolog in the n^th genome. To define an evolutionary distance between two sequences an alignment between two sequences is performed. Such alignments can be carried out by any number of algorithms known in the art (for examples, see those described above). The evolution is represented by a Markov process with substitution rates, over a fixed interval of time, given by a conditional probability matrix: p(αα → αα ') where αα and αα ' are any amino acids. One way to construct such a matrix is to convert the BLOSUM62 amino acid substitutions matrix (or any other amino acid substitution matrix, e.g., PAM100, PAM250) from a log odds matrix to a conditional probability (or transition) matrix:

P(i -→j) is the probability that amino acid /^' will be replaced by amino acid j through point mutations according to the BLOSUM62 scores. T ep/s are the abundances of amino acid j and are computed by solving the 20 linear equations given by the normalization conditions that: P_B(i → j) = X . (2)

The probability of this process is computed to account for the observed alignment by taking the product of the conditional probabilities for each aligned pair:

A family of evolutionary models is then tested by taking powers of the conditional probability matrix: p -p^a(aa→aa '). The power α that maximized P is defined to be the evolutionary distance.

Many other schemes may be imagined to deduce the evolutionary distance between two sequences. For example, one might simply count the number of positions in the sequence where the two proteins have adapted different amino acids. Although the phylogenetic history of an organism can be presented as a vector

(as described above), the phylogenetic profiles need not be vectors, but may be represented by matrices. This matrix includes all the pair wise distances between a group of homologous protein, each one from a different organism. Similarly, phylogenetic profiles could be represented as evolutionary trees of homologous proteins. Functional proteins could then be clustered or grouped by matching similar trees, rather than vectors or matrices.

In order to predict function, different proteins are grouped or clustered according to the similarity of their phylogenetic profiles. Similar profiles indicate a conelated pattern of inheritance, and by implication, functional linkage.

Grouping or clustering may be accomplished in many ways. The simplest is to compute the Euclidean distance between two profiles. Another method is to compute a conelation coefficient to quantify the similarity between two profiles. All profiles within a specified distance of the query profile are considered to be a cluster or group.

Typically a genome database will be used as a source of sequence information. Where the genome database contains only the nucleic acid sequence that sequence is translated to an amino acid sequence in frame (if known) or in all frames if unknown. Direct comparison of the nucleic acid sequences of two or more organisms may be feasible but will likely be more difficult due to the degeneracy of the genetic code. Programs capable of translating a nucleic acid sequence are known in the art or easily programmed by those of skill in the art to recognize a codon sequence for each amino acid.

The phylogenetic profile provides an indication of those proteins in each of the at least two organisms that share some degree of homology. Such a comparison can be done by any number of alignment algorithms known in the art or easily developed by one skilled in the art (see, for example, those listed above, e.g., BLAST, FASTA etc.) In addition, thresholds can be set regarding a required degree of homology. Each protein is then grouped at 224 with related proteins that share a similar phylogenetic profile using grouping algorithms. "Functionally-, Structurally- or Metabolically- Linked" Method

The "physiologic linkage" method is a computational method that detects (i.e., identifies) proteins, and the genes that encode them, that participate in a common functional pathway (e.g., cell motility or cell division), that participate in the synthesis of the same or a similar stractural complex (e.g., a cell wall) or participate in the same or similar metabolic pathway (e.g., glycolysis, lipid synthesis, and the like). Proteins within these common functional pathway groups are examples of "functionally linked" proteins. Having a common functional "goal" they evolve in a conelated fashion. Thus, "homologs" in different organisms can be comparatively identified. While these detection methods are very effective in identifying functional homologues in the same subset of organisms, functional linkages can be made between widely genetically disparate organisms.

In one aspect, metabolic pathways are defined as links between proteins that operate in the same metabolic pathway that can be identified by sequence identity searching, e.g., by performing a BLAST search to find top-scoring polypeptides with high similarity (BLAST alignment E-value < 10^" ) to polypeptides identified in a known pathway. For example, M. tuberculosis proteins were so analyzed against E. coli proteins; MTB proteins whose E. coli homologs (i.e., having high similarity by BLAST alignment) act adjacently in metabolic pathways as defined in the EcoCyc database (see, e.g., Kaφ (1998) Nucleic Acids Res. 26:50-53) were identified.

In another example, flagellar proteins are found in bacteria that possess flagella but not in other organisms. Accordingly, if two proteins have homologs in the same subset of fully sequenced organisms, they are likely to be functionally linked. The methods of the invention use this concept to systematically map links between all the proteins coded by a genome.

Typically, functionally linked proteins have no amino acid sequence similarity with each other and, therefore, cannot be linked by conventional sequence alignment techniques. Accordingly, the methods of the invention identify drag targets that could not be identified using conventional sequence comparison (i.e., sequence homology or sequence identity) techniques.

Prediction of functionally linked proteins by the "phylogenetic method" can also be used in conjunction with the "domain fusion" or "Rosetta Stone" method and also can be filtered by other methods that predict functionally linked proteins, such as the protein phylogenetic profile method or the analysis of correlated mRNA expression patterns. It was found that filtering by these two methods for the Rosetta Stone prediction for S. cerevisiae, that proteins predicted to be functionally linked by two or more of these three methods were as likely to be functionally related as proteins that were observed to physically interact by experimental techniques like yeast 2-hybrid methods or co-immunoprecipitation methods.

For example, a combination of these methods of prediction can be used to establish links between proteins of closely related function. The methods of the invention (i.e., the "Rosetta Stone" method and the "phylogenetic profile" method) can be combined with one another or with other protein prediction methods known in the art; see, for example, Eisen (1998) "Cluster analysis and display of genome-wide expression partners," Proc. Natl. Acad. Sci. USA, 95:14863-14868.

The various techniques, methods, and variations thereof described can be implemented in part or in whole using computer-based systems and methods. Additionally, computer-based systems and methods can be used to augment or enhance the functionality described above, increase the speed at which the functions can be performed, and provide additional features and aspects as a part of or in addition to those of the invention described elsewhere in this document. Various computer-based systems, methods, and implementations in accordance with this technology are described herein.

Proteins linked to current drug targets The invention also provides a novel method for identifying a polypeptide, or the nucleic acid sequence that encodes it, that is a target for a drag. The method analyzes the functional relationship between at least two sequences, wherein at least one of the sequences is a known target of a drug or encodes a polypeptide drug target. The method comprises identifying proteins, and the genes that encode them, that are functionally linked to the targets of known drugs. The functional linkage is determined by using the "domain fusion" method, the "phylogenetic profile" method or the "physiologic linkage" method, or a combination thereof, as described herein.

Thus, this aspect of the invention provides methods identifying drug targets from among all or a subset of genes in a genome using computationally-determined functional linkages. In one implementation of the method, functional linkages are calculated using the "domain fusion" method, the "phylogenetic profile" method or the "physiologic linkage" method, or a combination thereof, between all "query genome genes." Next, each set of genes predicted to be functionally linked to either a known drag target or to a sequence homolog or ortholog (defined below) to a known drug target are examined. These proteins (and the nucleic acids that encode them) are functionally linked to known drug targets; thus, they are operating in the same pathways or systems targeted by the known drag. Accordingly, the methods of the invention have identified them as drug targets.

This method is particularly effective for identifying drug targets in pathogens, such as microorganisms, e.g., bacteria, viruses and the like. This method allows for the identification of novel drug targets that cannot be identified by other techniques, such as traditional sequence homology or sequence identity comparison techniques. Several known drag targets in M tuberculosis were used with the methods of the invention to use functional linkages to identify potential new drug targets in the same pathways as the known drug targets.

There are very few drags that are effective for anti-tuberculosis therapy, since the complex lipid-rich mycobacterial cell wall is impermeable to many antibacterial agents. Additionally, single- and multi-drag resistance is rapidly emerging against these drugs. To address this issue, the methods of the invention were used to identify Mycobacterium tuberculosis (MTB or TB) proteins that are functionally linked to the targets of known drugs. Inhibiting these proteins should have the same effect on the organism as the drag, since the same processes or pathways would be disrupted. Targeting multiple components of a given biochemical pathway would also diminish the opportunity for the development of resistance because various related proteins would have to mutate against inhibitors while preserving the overall functionality of the pathway.

A list of targets of essential anti-TB drugs (World Health Organization, Geneva, Switzerland) was compiled. The anti-TB drugs included isoniazid, rifampicin, ethambutol, streptomycin, pyrazinamide and thiacetazone. Although not enough is known about the molecular basis of action of the latter two, the functional linkages of the known drag targets was examined.

Isoniazid. This is one of the most widely used of all anti-tuberculosis drugs. It is believed that the compound is activated by the catalase-peroxidase KatG. Once activated, it then attaches to a nicotinamide adenine dinucleotide bound to the enoyl-acyl caπier protein reductase InhA, resulting in the inhibition of mycolic acid biosynthesis Rozwarski (1998) Science 279:98-102.

Using the "phylogenetic profile, the inhA gene was "linked," or functionally associated with, to two polyketide synthases, pksl and pks6 (Figure 1), both of which contain acyl carrier protein motifs. The polyketide synthase pks6 is in turn known from established metabolic pathways to be linked to fatty acid biosynthesis gene accD3. Further, pks6 is linked to fadD28 and to the operon containing the genes ppsA-E, all recently reported to be crucial for bacterial replication in host lungs (see, e.g., Cox (1999) Nature 402:79-83). The inhA gene was also linked to an operon encoding two putative oxidoreductases and a gene of entirely unknown function. The inhA gene was further linked to a second operon that includes pepR and gpsl. PepR is a protease whose Bacillus subtilis homolog is adjacent to the genes coding for enzymes that synthesize diaminopimelate, a component of the cell wall incoφorated by the murE gene product and diaminopicolinate (see, e.g., Chen (1993) J. Biol. Chem. 268:9448-9465). PepR is an ortholog of an essential yeast gene and is likely to be essential for MTB (see below). Gpsl is a putative multifunctional enzyme involved in guanosine pentaphosphate synthesis and polyribonucleotide nucleotidyltransfer. The high reliability of the predicted functional link between gpsl and pepR and the absence of eukaryotic homologs suggests that gpsl could be a promising target for drag design. Rifampicin. This compound, along with the related rifabutin and KRM- 1648 are believed to act by directly targeting the RNA polymerase β-subunit (φoB) given that 96% of resistant isolates were found to have mutations of various types in a limited region of the φoB gene (see, e.g., Yang (1998) J. Antimicrob. Chemother. 42:621-628).

Using the methods of the invention, as expected, functional linkages were found to another RNA polymerase subunit, φoC, as well as to various tRNA synthases and ribosomal proteins. However, no functional links to uncharacterized proteins were found. Ethambutol. This drag is effective against tuberculosis when used in combination with isoniazid. It is believed that the drug interacts with the EmbB protein, a probable arabinosyl-transferase, inhibiting the biosynthesis of arabinan, a component of cell- envelope lipids. As with rifampicin, the evidence for this interaction is indirect, since mutations in the embB gene are responsible for ethambutol resistance (see, e.g., Lety (1997) Antimicrob. Agents Chemother. 41 :2629-2633).

The "gene proximity" method conectly clusters embB with embA (Rv3794). This cluster is linked to a set of mostly uncharacterized genes by the "phylogenetic profile" method; see Figure 2, which shows an analysis of EmbB, the target for the anti-tuberculosis drug Ethambutol, and shows functional linkages to genes mostly of unknown function but with some indications of localization at the bacterial membrane.

Two of the uncharacterized genes, Rvl706c and Rvl800, belong to the abundant PE/PPE family of proteins hypothesized to be a source of antigenic variation with the potential ability to interfere with immune responses by inhibiting antigen processing (see, e.g., Cole (1998) Nature 393, 537-544). A third uncharacterized gene, Rvl967 belongs to the one of the four copies of the mce operon. This operon consists of eight genes coding for integral membrane proteins and proteins that have N-terminal signal sequences or hydrophobic segments and are believed to be involved in pathogenicity (see, e.g., Cole (1998) supra). Rv0528 codes for a hypothetical membrane protein and Rv2159c conesponds to the murF gene, which participates in the biosynthesis of peptidoglycan precursors.

The majority of the "links," or functionally associated sequences, involved proteins associated with processes related to the bacterial cell wall (with the possible exception of atsA and the putative choline dehydrogenase Rvl279, whose relationship to these processes is not immediately obvious). The proteins of unknown function are therefore also expected to play some role in these processes and are thus of interest as potential drug targets. Streptomycin. This drag acts by binding to the 16S rRNA and inhibits protein synthesis. Resistance to this compound emerges from mutations in the conesponding gene (ns), as well as in the gene encoding for the ribosomal protein S12 (φsL). Disruptions to RpsL effect streptomycin resistance by altering the higher order structure of 16S rRNA (see, e.g., Sreevatsan (1996) Antimicrob. Agents Chemother. 40: 1024-1026).

Although streptomycin doesn't directly target RpsL, the functional links generated for this protein was examined, as any target whose inhibition will ultimately disrupt bacterial protein synthesis is likely to be an effective antigrowth/ anti-microbial target. As with the rifampicin target, the only functional linkages found for this protein were the expected protein synthesis-related proteins, including large ribosomal subunit proteins L2, L5, LI 1, and L14; small ribosomal subunit proteins S4, S5, S7, S8, and SI 1; elongation factors fusA and Ef-Tu; the chaperones GroEL, clpB and ftsH; and the Clp protease subunits clpC and clpX.

Proteins linked to cell-wall related proteins The invention also provides a novel method for identifying a nucleic acid or a polypeptide sequence in an organism that is linked to a cell-wall related protein. The method analyzes the functional relationship between at least two sequences, wherein at least one of the sequences is a cell-wall related protein, or, the sequence is a nucleic acid sequence that encodes a cell-wall related protein. The method comprises identifying proteins, and the genes that encode them, that are functionally linked to a cell-wall related protein. The functional linkage is determined by using the "domain fusion" method, the "phylogenetic profile" method or the "physiologic linkage" method, or a combination thereof, as described herein.

Approximately eleven M. tuberculosis proteins are indicated by sequence homology to be penicillin-binding proteins, thought to synthesize peptidoglycan in the course of cell elongation and cell wall metabolism (see, e.g., Broome-Smith (1985) Eur. J. Biochem. 147:437-446). Using the methods of the invention, the functional linkages found for these proteins map out many of the known cell wall synthetic enzymes and reveal more than 10 proteins of unknown function that may also participate in cell wall metabolism. Figure 3 shows an analysis of five of the approximately eleven MTB proteins presumed to bind penicillin to reveal functional linkages to various potential operons consisting of genes involved in various aspects of cell wall metabolism, including cell shape determination and peptidoglycan biosynthesis, as well more than ten genes of unknown function, which we can now associate with cell wall metabolism.

Three of the proteins (pbpA, pbpB, and ponAl) reside in conserved gene clusters, presumably operons. Other genes in the clusters around pbpA and pbpB are also implicated in cell wall metabolism. For example, pbpA resides next to rodA, a membrane- associated protein whose E. coli homolog determines cell shape and is required for enzymatic activity of penicillin binding proteins (see, e.g., Matsuzawa (1989) J. Bacteriol. 171 :558- 560). Likewise, pbpB resides next to six peptidoglycan biosynthesis genes and the two septum and cell wall formation proteins ftsW and ftsZ.

Two additional gene clusters were linked to these penicillin binding proteins by either the "phylogenetic profile" or "Rosetta Stone" pattern methods of the invention. One cluster is composed of the peptidoglycan synthetic protein murB and a putative membrane protein of unknown function that the functional linkages suggest is involved in cell wall metabolism. The second gene cluster contains four genes, three of which are predicted to reside in the cell membrane or envelope. Therefore, the uncharacterized genes in these clusters are likely to be involved in cell wall metabolism, closely related to the function of the penicillin binding proteins and are therefore promising drug targets.

Another gene linked to cell wall metabolism by the computationally-derived linkage methods of the invention is gcpΕ, see Figure 4, which shows that the uncharacterized gene gcpΕ, known to be essential for bacterial survival (see, e.g., Baker (1992) FΕMS Microbiol. Lett. 73:175-180), is predicted to be involved in cell wall metabolism through its functional links to a putative membrane protein and two murein hydrolase genes, lytBl and lytB2, involved in cell separation. The genes forming a putative operon with gcpΕ are proposed as potential drug targets. The functional linkages place gcpΕ in a conserved gene cluster with two genes of unknown function, one of which encodes a membrane protein. However, the three genes show conelated inheritance with two homologs of lytB, an E. coli gene involved in penicillin tolerance (see, e.g, Gustafson (1993) J. Bacteriol. 175:1203-1205) and recently shown to encode a murein hydrolase essential for cell separation (see, e.g., Garcia (1999) Mol. Microbiol. 31 :1275-1277). The uncharacterized proteins from this cluster are therefore expected to participate in processes similar to GcpE and might therefore be promising drug targets.

Proteins linked to potentially novel pathways

The invention also provides a novel method for identifying a polypeptide, or a nucleic acid that encodes it, that is linked to potentially novel biochemical (e.g., biosynthetic, metabolic) pathways. The method analyzes the functional relationship between at least two sequences, wherein at least one of the sequences is associated with a biochemical pathway, such as a pathway in a microorganism that enables the pathogen to evade an immune process. The method comprises identifying proteins, and the genes that encode them, that are functionally linked to the pathway-linked sequences. The functional linkage is determined by using the "domain fusion" method, the "phylogenetic profile" method or the "physiologic linkage" method, or a combination thereof, as described herein.

For example, the htrA gene encodes for a putative heat shock protein homologous to HtrA from Salmonella typhimurium, a serine protease that degrades aberrant periplasmic proteins. Mutations in this protein have been linked with reduced viability in host macrophages (see, e.g., Johnson (1991) Mol. Microbiol. 5:401-407). Thus, it was decided to investigate the function of htrA. Using the methods of the invention, results indicated that the htrA protein is part of a process that has not yet been characterized. The gene is predicted with very high reliability to function with the uncharacterized gene Rv 1224c, see Figure 5, which shows the involvement of htrA in a potentially novel pathway and the gene encoding the putative heat shock protein HtrA is functionally linked to a set of genes mostly of unknown function, suggesting the existence of a novel pathway. The partially characterized proteins suggest that the pathway relates to membrane-associated processes such as signaling and/or transport. The lack of eukaryotic homologs for most of the genes linked to htrA, suggests that proteins of this pathway could be promising drag targets.

Through its phylogenetic profile, htrA is linked to a group of uncharacterized proteins, including a putative lipid esterase (Rvl900c), an ABC transporter (Rv3783) and the uncharacterized protein Rvl216c, which has weak homology to the laminin B receptor of Xenopus laevis, suggesting that it might be a membrane protein. From this analysis, it can be concluded that htrA is part of a novel pathway that involves membrane-associated processes, such as signaling and/or transport. Because the majority of the proteins linked to htrA have no eukaryotic homologs, and given the importance of htrA in S. typhimurium pathogenesis, this pathway represents another potential source of novel targets for anti-tuberculosis drugs.

Proteins linked to essential proteins The invention also provides a novel method for identifying a polypeptide, or the nucleic acid sequence that encodes it, that is linked to an essential protein (e.g., a protein necessary for the growth of an organism, such as a bacterium). The method analyzes the functional relationship between at least two sequences, wherein at least one of the sequences is linked to an essential protein, or, the sequence is a nucleic acid sequence that itself is essential or encodes a polypeptide linked to an essential protein. The functional linkage is determined by using the "domain fusion" method, the "phylogenetic profile" method or the "physiologic linkage" method, or a combination thereof, as described herein.

For example, the MIPS database (Munich Information Center for Protein Sequences; MIPS provides access through its WWW server to a spectrum of generic databases, including PEDANT, MYGD, MATD, MEST, the PIR-International Protein

Sequence Database, the protein family database PROTFAM, the MITOP database, and the all-against-all FASTA database; see, e.g., Mewes (1999) Nucleic Acids Res. 27:44-48) contains a list of 734 genes that are essential for Saccharomyces cerevisiae viability (see, e.g., Mewes (1999) supra). A list of Mycobacterium tuberculosis genes orthologous to these essential genes was generated. Using the methods of the invention, 60 such genes were found. The products of these genes have a high likelihood of also being essential to the tuberculosis bacterium and therefore could be promising therapeutic targets. Furthermore, since the list of essential genes came from a eukaryote, there is a significant chance that these genes would also be found in the human genome. Automatic Method to Identify Drug Targets from Functional Linkages

One aspect of the invention provides a computational method to identify potential drag targets among the proteins expressed by a genome. This aspect takes advantage of the functional linkages calculated between genes in a genome using the methods described herein, as well as the detection of sequence homology and the knowledge of a set of lethal or "essential" genes in one or more organisms. To identify drag targets in a query genome, the sequence homology between all of the genes in that genome and all of the genes in the genome of an organism for which essential genes are known is calculated. For example, as discussed herein, the query genome is Mycobacterium tuberculosis (TB) and the genome with known essentials is the yeast S. cerevisiae. Sequence homology between all TB genes and all yeast genes was calculated using the methods of the invention.

"Equivalent" or "orthologous" genes were also identified by another aspect of the invention that comprises doing a reverse sequence search (e.g., yeast vs. TB) and then choosing pairs of genes that are the symmetric best-scoring sequence search. In one exemplary aspect, MTB orthologs of Saccharomyces cerevisiae genes were generated by finding all pairs of genes (TBj,SC_j) where TB, was the top hit from a BLAST search of the yeast gene SC, against the MTB genome, SC_j was the top hit from a BLAST search of the MTB gene TB, against the Saccharomyces cerevisiae genome and both top hits had a BLAST E-value <= lxlO^"5. For example, a TB gene is an ortholog of a yeast gene if the yeast gene is the best scoring sequence match when yeast is searched with the TB gene, and the TB gene is the best scoring sequence match when TB is searched with the yeast gene. We define these "symmetric" pairs as "orthologs."

After identifying orthologs between the query genome and the genome with known essential genes, a set of query genome genes that are orthologs of known essential genes in the other genome was chosen. These genes were designated the set of "putative essentials". For the puφoses of the algorithm of the invention, these query genome genes are assumed to be essential genes, since they are the equivalents of essential genes in another genome. These genes act as "markers" or indicators of essential pathways in the query genome. One could supplement this set with genes already known to be essential in the query organism. Functional linkages (determined by the methods of the invention) between all query genome genes were examined. The query genome genes linked to all of the putative essential genes were examined. This set of genes was designated as the "predicted members of essential pathways." These genes are likely to be involved in important pathways, since the (predicted) pathways have members that are putative essentials. Lastly, the method removes from the set of genes in predicted essential pathways all of those genes that have sequence homology to eukaryotic genes or proteins. The genes that remain after this filtering step are the predicted drag targets for the query organism.

As a benchmark, this method was applied to the M tuberculosis genome. Of the over 3900 genes in TB, 11 were identified as potential drug targets. Comparing this list of 11 predicted targets to the less than 10 known drug anti-TB drag targets, one gene was a known drug target and one was linked to a known drug target. Accordingly, the algorithm of the invention performed statistically significantly much better than a random choice of genes. A rough estimate of statistical significance suggests that one would expect to see 2 of 10 known drug targets in a sample of 11 out of 3900 genes only 3.8 times out of 10,000 trials (probability of occuning by random chance of 3.8 x 10^"4). Therefore, this embodiment of the method is an entirely computational algorithm drawing on the demonstrated ability of the general methods of the invention to predict functional linkages between genes and to effectively identify drag targets in bacteria. The effectiveness of this method to identify novel drug targets was clearly demonstrated when the algorithm was applied to the M. tuberculosis genome.

The specific inhibition of the MTB homologs might be difficult. To address this issue, using the methods of the invention, functional links to the essential genes were searched. Functional links were selected which either do not have homologs in yeast, or the enzymatic activity of their products are known to be absent in human cells. Using the highest confidence data, functional links for 23 of the genes (indicated in bold in Table 1) were found.

Table 1. MTB orthologs of essential yeast proteins.

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P H U Name Gene Comments Name Gene Comments PH RvOOOS* gyrB DNA gyrase subunit B Rv2101 helZ probable helicase, Snf2/Rad54 family RvOOHc p nB serine-threoninc protein kinase Rv21 10c prcB proteasome [beta]-type subunit 2

Rv0032 bioF2 C-terminal similar to B. subtilis BioF Rv21 18c B2 I 26_C1_165 (83.6%)

Rv0350 dnaK 70 kD heat shot k protein, chromosome replication Rv2438c similar to YHN4 YEAST P38795

Rv0363c fba fructose bispho.sphate aldolase Rv2439c proB glutamate 5 -kinase

Rv0435c ATPase of AAΛ-family Rv2448c valS valyl-tRNA synthase

Rv0436c pssA CDP-diacylglycerol-serine o-phosphatidyltransferase Rv2509 putative oxidoreductase

Rv0440 groEL2 60 kD chaperon in 2 Rv2524c fas fatty acid synthase

Rv0489 gp phosphoglycerale mutase I Rv2555c alaS alanyl-tRNA synthase

Rv0490 senX3 sensor histidine kinase Rv2580c hisS histidyl-tRNA synthase o

Rv0500 proC pyrroline-5-carl'θxylate reductase Rv2614c thrS threonyl-tRNA synthase

Rv0667 rpoB [beta] subunit of RNA polymerase Rv2697c dut deoxyuridine tri phosphatase

Rv0668 rpoC [beta]¹ subunit of RNA polymerase Rv2782c pepR protease/peptidase, M16 family (insulinase)

Rv0764c possible lanostcrol 14-demethylase cytochrome P450 Rv2793c truB tRNA pseudouridine 55 synthase

Rv0861c probable DNA helicase Rv2922c smc member of Smcl/Cut3/Cutl4 family

RvlOlO ksgA 16S rRNA dimcthyltransferase Rv2925c rnc RNΛse III

RvllOόc probable cholesterol dehydrogenase Rv3014c ligA DNA ligase

Rvl229c mrp similar to MRP/NBP35 ATP-binding proteins Rv3025c NifS-like protein

Rv1239c corA probable magnesium and cobalt transport protein Rv3080c pknK serine-threonine protein kinase

RvI294 thrA homoserine dehydrogenase Rv3106 fprA adrenodoxin and NADPH ferredoxin reductase

Rvl323 fadA4 acetyl-CoA C-acetyltransferase (aka thiL) Rv3255c man A mannose-6-phosphate isomerase

Rvl389 gmk putative guanyl.ite kinase Rv3264c rmlA2 glucose- 1 -phosphate thymidyltransferase

Rvl407 f u similar to Fmu protein Rv3418c groES 10 kD chaperone

Rvl409 ribG riboflavin biosj nthesis Rv3490 otsA probable [alpha],-trehalose-phosphate synthase

Rvl6l7 pykA pyruvate kinase Rv3598c lysS lysyl-tRNA synthase

Rvl630 rpsA 30S ribosomal protein SI Rv3608c folP dihydropteroate synthase

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I ) Rvl745c similar to Q46822 ORF_0182 Rv3609c folE GTP cyclohydrolase I

Rv1844c gnd 6-phosphogluconate dehydrogenase (Gram -) Rv3721c dnaZX DNA polymerase III, [gamma] (dnaZ) and t (dnaX)

Rvl981c nrdF ribonucleotide reductase small subunit Rv3834c serS seryl-tRNA synthase

Rv2092c helY probable helicase, Ski2 subfamily Rv3907c pcnA polynucleotide polymerase We follow the Sanger Centre naming convention for MTB genes.

* Genes for which high-confidence functional links were found shown in boldface

Eight of these were linked to 12 unique MTB genes that satisfied the criteria of the invention's methods (Table 1). Exemplary findings include:

(1) the gene folP, which encodes the enzyme dihydropteroate synthase (DHPS) known to be the target of sulfonamide antibacterial drags. Although it is found in some eukaryotes, DHPS activity is not found in human cells (see, e.g., Huovinen (1995)

Antimicrob. Agents Chemother. 39:279-2890.

(2) the product of the gene folK, a 7,8-dihydro-6-hydroxymethyl- pterinpyrophosphokinase, has recently been proposed as a target for broad-spectrum antibacterial drugs (see, e.g., Stammers (1999) FEBS Lett. 456:49-53). (3) the gene gpsl, is not only strongly linked to the essential yeast gene pepR, but it is also functionally linked to inhA, the target of the drag isoniazid (see above), making it a very compelling candidate for drug design.

Table 2. Subset of genes from Table 1 that are functionally linked to genes without yeast homologs.

* Genes without yeast homologs shown in boldface

* DHPS activity is found in some eukaryotic cells but not in human cells

In summary, the methods of the invention allowed identification of this combination of functional linkages to essential genes. This information, together with the lack of eukaryotic homologs for these genes, makes this group of proteins promising drug targets, particularly because their inhibition is expected to disrupt vital bacterial processes with a low likelihood of toxicity from the inhibition of a host equivalent. Computer Implementation

The various techniques, methods, and aspects of the invention described herein can be implemented in part or in whole using computer-based systems and methods. Additionally, computer-based systems and methods can be used to augment or enhance the functionalities and algorithms described herein, increase the speed at which the functions can be performed, and provide additional features and aspects as a part of or in addition to those of the invention described elsewhere in this document. Various exemplary computer-based systems, methods and implementations in accordance with the above-described technology are presented herein. The processor-based system can include a main memory, such as a random access memory (RAM), and can also include a secondary memory. The secondary memory can include, for example, a hard disk drive and/or a removable storage drive, representing a floppy disk drive, a magnetic tape drive, an optical disk drive, etc. The removable storage drive reads from and/or writes to a removable storage medium. Removable storage media can be a floppy disk magnetic tape, an optical disk, and the like, which can be read by and written to by removable storage drive. The removable storage media can includes a computer usable storage medium having stored therein computer software and/or data.

In alternative embodiments, secondary memory may include other similar means for allowing computer programs or other instructions to be loaded into a computer system. Such means can include, for example, a removable storage unit and an interface.

Examples of such can include a program cartridge and cartridge interface (such as the found in video game devices), a movable memory chip (such as an EPROM, or PROM) and associated socket, and other removable storage units and interfaces that allow software and data to be transfened from the removable storage unit to the computer system. The computer system can also include a communications interface.

Communications interfaces allow software and data to be transfened between computer system and external devices. Examples of communications interfaces include modems, network interfaces (such as, for example, an Ethernet card), communications ports, PCMCIA slots and cards, and the like. Software and data transfened via a communications interface can be in the form of signals that can be electronic, electromagnetic, optical or other signals capable of being received by a communications interface. These signals can be provided to communications interface via a channel capable of carrying signals and can be implemented using a wireless medium, wire or cable, fiber optics or other communications medium. Some examples of a channel can include a phone line, a cellular phone link, an RF link, a network interface, and other communications channels. As used herein, the terms "computer program medium" and "computer usable medium" are used to generally refer to media such as a removable storage device, a disk capable of installation in a disk drive, and signals on a channel, or equivalents thereof. These computer program products are means for providing software or program instructions to computer systems. Computer programs (also called computer control logic) can be stored in main memory and/or secondary memory. Computer programs can also be received via a communications interface. Such computer programs, when executed, enable the computer system to perform the features of the present invention as discussed herein. Computer programs, when executed, enable the processor to perform the features of the present invention. Accordingly, in one aspect of the invention, such computer programs represent controllers of the computer system.

In another aspect of the invention the methods and algorithms are implemented using software, the software may be stored in, or transmitted via, a computer program product and loaded into a computer system using a removable storage drive, hard drive or communications interface. The control logic (software), when executed by the processor, causes the processor to perform the functions of the invention as described herein.

In another aspect, the elements are implemented primarily in hardware using, for example, hardware components such as PALs, application specific integrated circuits (ASICs) or other hardware components. Implementation of a hardware state machine so as to perform the functions described herein will be apparent to person skilled in the relevant art(s). In yet another embodiment, elements are implanted using a combination of both hardware and software.

In another aspect, the computer-based methods can be accessed or implemented over the World Wide Web by providing access via a Web Page to the methods of the present invention. Accordingly, the Web Page is identified by a Universal Resource Locator (URL). The URL denotes both the server machine, and the particular file or page on that machine. In this embodiment, it is envisioned that a consumer or client computer system interacts with a browser to select a particular URL, which in turn causes the browser to send a request for that URL or page to the server identified in the URL. Typically the server responds to the request by retrieving the requested page, and transmitting the data for that page back to the requesting client computer system (the client/server interaction is typically performed in accordance with the hypertext transport protocol ("HTTP")). The selected page is then displayed to the user on the client's display screen. The client may then cause the server containing a computer program of the present invention to launch an application comprising a method of the invention, for example, to identify a nucleic acid or a polypeptide sequence that may be a target for a drug comprising the steps of (a) providing a first nucleic acid or a polypeptide sequence that is known to be a drag target; (b) providing an algorithm capable analyzing a functional relationship between nucleic acid or polypeptide sequences selected from the group consisting of a "domain fusion" method, a "phylogenetic profile" method and a "physiologic linkage" method; and, (c) comparing the first nucleic acid or the polypeptide drag target sequence to a plurality of sequences using at least one algorithm to identify a second sequence that has a functional relationship to the first sequence, thereby identifying a nucleic acid or a polypeptide sequence that may be a target for a drug, based on a query sequence provided by the client.

Nucleic Acids and Polypeptides

The invention also provides isolated nucleic acids and polypeptides comprising the sequences as set forth in Table 3 and Table 4 (below). As used herein,

"isolated," when refening to a molecule or composition, such as, e.g., an isolated infected cell comprising a nucleic acid sequence derived from a library of the invention, means that the molecule or composition (including, e.g., a cell) is separated from at least one other compound, such as a protein, DNA, RNA, or other contaminants with which it is associated in vivo or in its naturally occurring state. Thus, a nucleic acid or polypeptide or peptide sequence is considered isolated when it has been isolated from any other component with which it is naturally associated. An isolated composition can, however, also be substantially pure. An isolated composition can be in a homogeneous state. It can be in a dry or an aqueous solution. Purity and homogeneity can be determined, e.g., using any analytical chemistry technique, as described herein. The term "nucleic acid" or "nucleic acid sequence" refers to a deoxy- ribonucleotide or ribonucleotide oligonucleotide, including single- or double-stranded, or coding or non-coding (e.g., "antisense") forms. The term encompasses nucleic acids, i.e., oligonucleotides, containing known analogues of natural nucleotides. The term also encompasses nucleic-acid-like stractures with synthetic backbones, see e.g., Oligonucleotides and Analogues, a Practical Approach, ed. F. Eckstein, Oxford Univ. Press (1991); Antisense Strategies, Annals of the N.Y. Academy of Sciences, Vol 600, Eds. Baserga et al. (NYAS 1992); Milligan (1993) J. Med. Chem. 36:1923-1937; Antisense Research and Applications (1993, CRC Press), WO 97/03211; WO 96/39154; Mata (1997) Toxicol. Appl. Pharmacol. 144:189-197; Strauss-Soukup (1997) Biochemistry 36:8692-8698; Samstag (1996) Antisense

Nucleic Acid Drug Dev 6:153-156. As used herein, the "sequence" of a nucleic acid or gene refers to the order of nucleotides in the polynucleotide, including either or both strands (sense and antisense) of a double-stranded DNA molecule, e.g., the sequence of both the coding strand and its complement, or of a single-stranded nucleic acid molecule (sense or antisense). For example, in alternative embodiments, promoters drive the transcription of sense and/or antisense polynucleotide sequences of the invention, as exemplified by Table 3.

The terms "polypeptide," "protein," and "peptide" include compositions of the invention that also include "analogs," or "conservative variants" and "mimetics" ("peptidomimetics") with structures and activity that substantially conespond to the exemplary sequences, such as the sequences in Table 4. Thus, the terms "conservative variant" or "analog" or "mimetic" also refer to a polypeptide or peptide which has a modified amino acid sequence, such that the change(s) do not substantially alter the polypeptide' s (the conservative variant's) structure and/or activity (e.g., immunogenicity, ability to bind to human antibodies, etc.), as defined herein. These include conservatively modified variations of an amino acid sequence, i.e., amino acid substitutions, additions or deletions of those residues that are not critical for protein activity, or substitution of amino acids with residues having similar properties (e.g., acidic, basic, positively or negatively charged, polar or non- polar, etc.) such that the substitutions of even critical amino acids does not substantially alter structure and/or activity. Conservative substitution tables providing functionally similar amino acids are well known in the art. For example, one exemplary guideline to select conservative substitutions includes (original residue followed by exemplary substitution): ala/gly or ser; arg/ lys; asn/ gin or his; asp/glu; cys/ser; gln/asn; gly/asp; gly/ala or pro; his/asn or gin; ile/leu or val; leu/ile or val; lys/arg or gin or glu; met/leu or tyr or ile; phe/met or leu or tyr; ser/thr; thr/ser; trp/tyr; tyr/trp or phe; val/ile or leu. An alternative exemplary guideline uses the following six groups, each containing amino acids that are conservative substitutions for one another: 1) Alanine (A), Serine (S), Threonine (T); 2) Aspartic acid (D), Glutamic acid (E); 3) Asparagine (N), Glutamine (Q); 4) Arginine (R), Lysine (K); 5) Isoleucine (I), Leucine (L), Methionine (M), Valine (V); and 6) Phenylalanine (F), Tyrosine (Y), Tryptophan (W); (see also, e.g., Creighton (1984) Proteins, W.H. Freeman and Company; Schulz and Schimer (1979) Principles of Protein Stracture, Springer- Verlag). One of skill in the art will appreciate that the above-identified substitutions are not the only possible conservative substitutions. For example, for some purposes, one may regard all charged amino acids as conservative substitutions for each other whether they are positive or negative. In addition, individual substitutions, deletions or additions that alter, add or delete a single amino acid or a small percentage of amino acids in an encoded sequence can also be considered "conservatively modified variations."

The terms "mimetic" and "peptidomimetic" refer to a synthetic chemical compound that has substantially the same structural and/or functional characteristics of the polypeptides of the invention (e.g., ability to bind, or "capture," human antibodies in an ELISA). The mimetic can be either entirely composed of synthetic, non-natural analogues of amino acids, or, is a chimeric molecule of partly natural peptide amino acids and partly non- natural analogs of amino acids. The mimetic can also incorporate any amount of natural amino acid conservative substitutions as long as such substitutions also do not substantially alter the mimetics' stracture and/or activity. As with polypeptides of the invention which are conservative variants, routine experimentation will determine whether a mimetic is within the scope of the invention, i.e., that its structure and/or function is not substantially altered. Polypeptide mimetic compositions can contain any combination of non-natural stractural components, which are typically from three stractural groups: a) residue linkage groups other than the natural amide bond ("peptide bond") linkages; b) non-natural residues in place of naturally occurring amino acid residues; or c) residues which induce secondary structural mimicry, i.e., to induce or stabilize a secondary stracture, e.g., a beta turn, gamma turn, beta sheet, alpha helix conformation, and the like. A polypeptide can be characterized as a mimetic when all or some of its residues are joined by chemical means other than natural peptide bonds. Individual peptidomimetic residues can be joined by peptide bonds, other chemical bonds or coupling means, such as, e.g., glutaraldehyde, N-hydroxysuccinimide esters, bifunctional maleimides, N,N'-dicyclohexylcarbodiimide (DCC) or N,N'- diisopropylcarbodiimide (DIC). Linking groups that can be an alternative to the traditional amide bond ("peptide bond") linkages include, e.g., ketomethylene (e.g., -C(=O)-CH - for - C(=O)-NH-), aminomethylene (CH₂-NH), ethylene, olefin (CH=CH), ether (CH₂-O), thioether (CH₂-S), tetrazole (CN₄-), thiazole, retroamide, thioamide, or ester (see, e.g., Spatola (1983) in Chemistry and Biochemistry of Amino Acids, Peptides and Proteins, Vol. 7, pp 267-357, "Peptide Backbone Modifications," Marcell Dekker, NY). A polypeptide can also be characterized as a mimetic by containing all or some non-natural residues in place of naturally occurring amino acid residues; non-natural residues are well described in the scientific and patent literature.

The invention comprises nucleic acids comprising sequences as set forth in Table 3, or comprising nucleic acids encoding the polypeptides as set forth in Table 4, operably linked to a transcriptional regulatory sequence. As used herein, the term "operably linked," refers to a functional relationship between two or more nucleic acid (e.g., DNA) segments. Typically, it refers to the functional relationship of a transcriptional regulatory sequence to a transcribed sequence. For example, a promoter (defined below) is operably linked to a coding sequence, such as a nucleic acid of the invention, if it stimulates or modulates the transcription of the coding sequence in an appropriate host cell or other expression system. Generally, promoter transcriptional regulatory sequences that are operably linked to a transcribed sequence are physically contiguous to the transcribed sequence, i.e., they are cw-acting. However, some transcriptional regulatory sequences, such as enhancers, need not be physically contiguous or located in close proximity to the coding sequences whose transcription they enhance. For example, in one embodiment, a promoter is operably linked to an ORF-containing nucleic acid sequence of the invention, as exemplified by, e.g., a nucleic acid sequence as set forth in Table 3.

As used herein, the term "promoter" includes all sequences capable of driving transcription of a coding sequence in an expression system. Thus, promoters used in the constructs of the invention include α^'s-acting transcriptional control elements and regulatory sequences that are involved in regulating or modulating the timing and/or rate of transcription of a nucleic acid of the invention. For example, a promoter can be a -acting transcriptional control element, including an enhancer, a promoter, a transcription terminator, an origin of replication, a chromosomal integration sequence, 5' and 3' untranslated regions, or an intronic sequence, which are involved in transcriptional regulation. These cw-acting sequences typically interact with proteins or other biomolecules to carry out (turn on/off, regulate, modulate, etc.) transcription.

The invention comprises expression cassettes comprising nucleic acids comprising sequences as set forth in Table 3, or comprising nucleic acids encoding the polypeptides as set forth in Table 4. The term "expression vector" refers to any recombinant expression system for the purpose of expressing a nucleic acid sequence of the invention in vitro or in vivo, constitutively or inducibly, in any cell, including prokaryotic, yeast, fungal, plant, insect or mammalian cell. The term includes linear or circular expression systems. The term includes expression systems that remain episomal or integrate into the host cell genome. The expression systems can have the ability to self-replicate or not, i.e., drive only transient expression in a cell. The term includes recombinant "expression cassettes" which contain only the minimum elements needed for transcription of the recombinant nucleic acid.

Alignment Analysis of Sequences

The nucleic acid and polypeptide sequences of the invention include genes and gene products identified and characterized by sequence identify analysis (i.e., by homology) using the exemplary nucleic acid and protein sequences of the invention, including, e.g., those set forth in Tables 3 and 4. In alternative aspects of the invention, nucleic acids and polypeptides within the scope of the invention include those having 98%, 95%, 90%, 85% or 80% sequence identity (homology) to the exemplary sequences as set forth in Tables 3 and 4.

For sequence comparison, typically one sequence acts as a reference sequence, to which test sequences are compared. When using a sequence comparison algorithm, test and reference sequences are entered into a computer, subsequence coordinates are designated, if necessary, and sequence algorithm program parameters are designated. Default program parameters are used unless alternative parameters are designated herein.

The sequence comparison algorithm then calculates the percent sequence identity for the test sequence(s) relative to the reference sequence, based on the designated or default program parameters. A "comparison window", as used herein, includes reference to a segment of any one of the number of contiguous positions selected from the group consisting of from 25 to 600, usually about 50 to about 200, more usually about 100 to about 150 in which a sequence may be compared to a reference sequence of the same number of contiguous positions after the two sequences are optimally aligned. Methods of alignment of sequences for comparison are well-known in the art. Optimal alignment of sequences for comparison can be conducted, e.g., by the local homology algorithm of Smith 8c Waterman, Adv. Appl. Math. 2:482 (1981), by the homology alignment algorithm of Needleman & Wunsch, J. Mol. Biol. 48:443 (1970), by the search for similarity method of Pearson & Lipman, Proc. Natl. Acad. Sci. USA

85:2444 (1988), by computerized implementations of these algorithms (CLUSTAL, GAP, BESTFIT, FASTA, and TFASTA in the Wisconsin Genetics Software Package, Genetics Computer Group, 575 Science Dr., Madison, WI), or by manual alignment and visual inspection. In one aspect of the invention (in the methods of the invention, and, to determine if a sequence is within the scope of the invention), a CLUSTAL algorithm is used, e.g., the CLUSTAL W program, see, e.g., Thompson (1994) Nuc. Acids Res. 22:4673-4680; Higgins (1996) Methods Enzymol 266:383-402. Variations can also be used, such as CLUSTAL X, see Jeanmougin (1998) Trends Biochem Sci 23:403-405; Thompson (1997) Nucleic Acids Res 25:4876-4882. In one aspect, the CLUSTAL W program described by Thompson (1994) supra, is used with the following parameters: K tuple (word) size: 1, window size: 5, scoring method: percentage, number of top diagonals: 5, gap penalty: 3, to determine whether a nucleic acid has sufficient sequence identity to an exemplary sequence to be with the scope of the invention. In another aspect, the algorithm PILEUP is used in the methods and to determine whether a nucleic acid has sufficient sequence identity to be with the scope of the invention. This program creates a multiple sequence alignment from a group of related sequences using progressive, pairwise alignments to show relationship and percent sequence identity. It also plots a tree or dendogram showing the clustering relationships used to create the alignment. PILEUP uses a simplification of the progressive alignment method of Feng & Doolittle, J. Mol. Evol. 35:351-360 (1987). The method used is similar to the method described by Higgins & Sharp, CABIOS 5:151-153 (1989). Using PILEUP, a reference sequence (e.g., an exemplary GCA-associated sequence of the invention) is compared to another sequence to determine the percent sequence identity relationship (i.e., that the second sequence is substantially identical and within the scope of the invention) using the following parameters: default gap weight (3.00), default gap length weight (0.10), and weighted end gaps. In one embodiment, PILEUP obtained from the GCG sequence analysis software package, e.g., version 7.0 (Devereaux(1984) Nuc. Acids Res. 12:387-395), using the parameters described therein, is used in the methods and to identify nucleic acids within the scope of the invention. In a another aspect, a BLAST algorithm is used (in the methods, e.g., to determine percent sequence identity (i.e., substantial similarity or identity) and whether a nucleic acid is within the scope of the invention), see, e.g., Altschul (1990) J

Mol. Biol. 215:403-410. Software for performing BLAST analyses is publicly available through the National Center for Biotechnology Information, NIH. This algorithm involves first identifying high scoring sequence pairs (HSPs) by identifying short words of length W in the query sequence, which either match or satisfy some positive-valued threshold score T when aligned with a word of the same length in a database sequence. T is refened to as the neighborhood word score threshold (Altschul (1990) supra). These initial neighborhood word hits act as seeds for initiating searches to find longer HSPs containing them. The word hits are then extended in both directions along each sequence for as far as the cumulative alignment score can be increased. Cumulative scores are calculated using, for nucleotide sequences, the parameters M (reward score for a pair of matching residues; always > 0) and

N (penalty score for mismatching residues, always < 0). For amino acid sequences, a scoring matrix is used to calculate the cumulative score. Extension of the word hits in each direction are halted when: the cumulative alignment score falls off by the quantity X from its maximum achieved value; the cumulative score goes to zero or below, due to the accumulation of one or more negative-scoring residue alignments; or the end of either sequence is reached. The BLAST algorithm parameters W, T, and X determine the sensitivity and speed of the alignment. In one embodiment, to determine if a nucleic acid sequence is within the scope of the invention, the BLASTN program (for nucleotide sequences) is used incorporating as defaults a wordlength (W) of 11, an expectation (E) of 10, M=5, N=4, and a comparison of both strands. For amino acid sequences, the BLASTP program uses as default parameters a wordlength (W) of 3, an expectation (E) of 10, and the BLOSUM62 scoring matrix (see, e.g., Henikoff (1989) Proc. Natl. Acad. Sci. USA 89:10915).

Hybridization for Identifying Nucleic Acids of the Invention

Nucleic acids within the scope of the invention include isolated or recombinant nucleic acids that specifically hybridize under stringent hybridization conditions to an exemplary nucleic acid of the invention (including a sequence encoding an exemplary polypeptide) as set forth in Tables 3 and 4. Stringent conditions are sequence-dependent and will be different in different circumstances. Longer sequences hybridize specifically at higher temperatures. An extensive guide to the hybridization of nucleic acids is found in, e.g., Tijssen (1993) infra. Generally, stringent conditions are selected to be about 5 to 10°C lower than the thermal melting point (Tm) for the specific sequence at a defined ionic strength and pH. The Tm is the temperature (under defined ionic strength, pH, and nucleic acid concentration) at which 50% of the probes complementary to the target hybridize to the target sequence at equilibrium (as the target sequences are present in excess, at Tm, 50% of the probes are occupied at equilibrium). Stringent conditions will be those in which the salt concentration is less than about 1.0 M sodium ion, typically about 0.01 to 1.0 M sodium ion concentration (or other salts) at pH 7.0 to 8.3 and the temperature is at least about 30°C for short probes (e.g., 10 to 50 nucleotides) and at least about 60°C for long probes (e.g., greater than 50 nucleotides). Stringent conditions may also be achieved with the addition of destabilizing agents such as formamide.

For selective or specific hybridization, a positive signal (e.g., identification of a nucleic acid of the invention) is about 10 times background hybridization. "Stringent" hybridization conditions that are used to identify substantially identical nucleic acids within the scope of the invention include hybridization in a buffer comprising 50% formamide, 5x SSC, and 1% SDS at 42°C, or hybridization in a buffer comprising 5x SSC and 1% SDS at 65°C, both with a wash of 0.2x SSC and 0.1% SDS at 65°C. Exemplary "moderately stringent hybridization conditions" include a hybridization in a buffer of 40% formamide, 1 M NaCI, and 1% SDS at 37°C, and a wash in IX SSC at 45°C. Those of ordinary skill will readily recognize that alternative but comparable hybridization and wash conditions can be utilized to provide conditions of similar stringency. Nucleic acids which do not hybridize to each other under stringent hybridization conditions are still substantially identical if the polypeptides which they encode are substantially identical. This may occur, e.g., when a copy of a nucleic acid is created using the maximum codon degeneracy permitted by the genetic code, as discussed herein (see discussion on "conservative substitutions"). However, the selection of a hybridization format is not critical - it is the stringency of the wash conditions that set forth the conditions that determine whether a nucleic acid is within the scope of the invention. Wash conditions used to identify nucleic acids within the scope of the invention include, e.g.: a salt concentration of about 0.02 molar at pH 7 and a temperature of at least about 50°C or about 55°C to about 60°C; or, a salt concentration of about 0.15 M NaCI at 72°C for about 15 minutes; or, a salt concentration of about 0.2X SSC at a temperature of at least about 50°C or about 55°C to about 60°C for about 15 to about 20 minutes; or, the hybridization complex is washed twice with a solution with a salt concentration of about 2X SSC containing 0.1% SDS at room temperature for 15 minutes and then washed twice by 0.1X SSC containing 0.1% SDS at 68°C for 15 minutes; or, equivalent conditions. See Sambrook, Tijssen and Ausubel (see below) for a description of SSC buffer and equivalent conditions.

General Techniques

The nucleic acid and polypeptide sequences of the invention and other nucleic acids used to practice this invention, whether RNA, cDNA, genomic DNA, vectors, viruses or hybrids thereof, may be isolated from a variety of sources, genetically engineered, amplified, and/or expressed recombinantly. Any recombinant expression system can be used, including, in addition to bacterial cells, e.g., mammalian, yeast, insect or plant cell expression systems.

Alternatively, these nucleic acids and polypeptides can be synthesized in vitro by well-known chemical synthesis techniques, as described in, e.g., Carrathers (1982) Cold Spring Harbor Symp. Quant. Biol. 47:411-418; Adams (1983) J. Am. Chem. Soc. 105:661;

Belousov (1997) Nucleic Acids Res. 25:3440-3444; Frenkel (1995) Free Radic. Biol. Med.

19:373-380; Blommers (1994) Biochemistry 33:7886-7896; Narang (1979) Meth. Enzymol.

68:90; Brown (1979) Meth. Enzymol. 68:109; Beaucage (1981) Tetra. Lett. 22:1859; U.S.

Patent No. 4,458,066. Techniques for the manipulation of nucleic acids, such as, e.g., generating mutations in sequences, subcloning, labeling probes, sequencing, hybridization and the like are well described in the scientific and patent literature, see, e.g., Sambrook, ed., MOLECULAR CLONING: A LABORATORY MANUAL (2ND ED.), Vols. 1-3, Cold Spring Harbor Laboratory, (1989); CURRENT PROTOCOLS IN MOLECULAR BIOLOGY, Ausubel, ed. John Wiley 8c Sons, Inc., New York (1997); LABORATORY TECHNIQUES IN BIOCHEMISTRY AND MOLECULAR BIOLOGY: HYBRIDIZATION WITH NUCLEIC ACID PROBES, Part I. Theory and Nucleic Acid Preparation, Tijssen, ed. Elsevier, N.Y. (1993).

Polypeptides and peptides of the invention can also be synthesized, whole or in part, using chemical methods well known in the art. See e.g., Caruthers (1980) Nucleic Acids Res. Symp. Ser. 215-223; Horn (1980) Nucleic Acids Res. Symp. Ser. 225-232; Banga, A.K., Therapeutic Peptides and Proteins, Formulation, Processing and Delivery

Systems (1995) Technomic Publishing Co., Lancaster, PA. For example, peptide synthesis can be performed using various solid-phase techniques (see e.g., Roberge (1995) Science 269:202; Menifield (1997) Methods Enzymol. 289:3-13) and automated synthesis may be achieved, e.g., using the ABI 431 A Peptide Synthesizer (Perkin Elmer) in accordance with the instructions provided by the manufacturer.

The skilled artisan will recognize that individual synthetic residues and polypeptides incorporating mimetics can be synthesized using a variety of procedures and methodologies, which are well described in the scientific and patent literature, e.g., Organic Syntheses Collective Volumes, Gilman, et al. (Eds) John Wiley & Sons, Inc., NY. Polypeptides incorporating mimetics can also be made using solid phase synthetic procedures, as described, e.g., by Di Marchi, et al., U.S. Pat. No. 5,422,426. Peptides and peptide mimetics of the invention can also be synthesized using combinatorial methodologies. Various techniques for generation of peptide and peptidomimetic libraries are well known, and include, e.g., multipin, tea bag, and split-couple-mix techniques; see, e.g., al-Obeidi (1998) Mol. Biotechnol. 9:205-223; Hraby (1997) Cun. Opin. Chem. Biol.

1 :114-119; Ostergaard (1997) Mol. Divers. 3:17-27; Ostresh (1996) Methods Enzymol. 267:220-234. Modified peptides of the invention can be further produced by chemical modification methods, see, e.g., Belousov (1997) Nucleic Acids Res. 25:3440-3444; Frenkel (1995) Free Radic. Biol. Med. 19:373-380; Blommers (1994) Biochemistry 33:7886-7896. Peptides and polypeptides of the invention can also be synthesized and expressed as fusion proteins with one or more additional domains linked thereto for, e.g., producing a more immunogenic peptide, to more readily isolate a recombinantly synthesized peptide, to identify and isolate antibodies and antibody-expressing B cells, and the like. Detection and purification facilitating domains include, e.g., metal chelating peptides such as polyhistidine tracts and histidine-tryptophan modules that allow purification on immobilized metals, protein A domains that allow purification on immobilized immunoglobulin, and the domain utilized in the FLAGS extension/affinity purification system (Immunex Corp, Seattle WA). The inclusion of a cleavable linker sequences such as Factor Xa or enterokinase (Invitrogen, San Diego CA) between the purification domain and GCA-associated peptide or polypeptide can be useful to facilitate purification. For example, an expression vector can include an epitope-encoding nucleic acid sequence linked to six histidine residues followed by a thioredoxin and an enterokinase cleavage site (see e.g., Williams (1995) Biochemistry 34:1787-1797; Dobeli (1998) Protein Expr. Purif. 12:404-414). The histidine residues facilitate detection and purification while the enterokinase cleavage site provides a means for purifying the epitope from the remainder of the fusion protein. Technology pertaining to vectors encoding fusion proteins and application of fusion proteins are well described in the scientific and patent literature, see e.g., Kroll (1993) DNA Cell. Biol., 12:441-53.

The invention provides antibodies that specifically bind to the polypeptides of the invention, as set forth in Table 4. These antibodies can be useful in the screening methods of the invention. The polypeptides or peptide can be conjugated to another molecule or can be administered with an adjuvant. The coding sequence can be part of an expression cassette or vector capable of expressing the immunogen in vivo, (see, e.g., Katsumi (1994) Hum. Gene Ther. 5:1335-9). Methods of producing polyclonal and monoclonal antibodies are known to those of skill in the art and described in the scientific and patent literature, see, e.g., Coligan, CURRENT PROTOCOLS IN IMMUNOLOGY, Wiley/Greene, NY (1991); Stites (eds.) BASIC AND CLINICAL IMMUNOLOGY (7th ed.) Lange Medical Publications, Los Altos, CA; Goding, MONOCLONAL ANTIBODIES: PRINCIPLES AND PRACTICE (2d ed.) Academic Press, New York, NY (1986); Harlow (1988) ANTIBODIES, A LABORATORY MANUAL, Cold Spring Harbor Publications, New York.

Antibodies also can be generated in vitro, e.g., using recombinant antibody binding site expressing phage display libraries, in addition to the traditional in vivo methods using animals. See, e.g., Huse (1989) Science 246:1275; Ward (1989) Nature 341 :544; Hoogenboom (1997) Trends Biotechnol. 15:62-70; Katz (1997) Annu. Rev. Biophys. Biomol. Struct. 26:27-45. Human antibodies can be generated in mice engineered to produce only human antibodies, as described by, e.g., U.S. Patent No. 5,877,397; 5,874,299; 5,789,650; and 5,939,598. B-cells from these mice can be immortalized using standard techniques (e.g., by fusing with an immortalizing cell line such as a myeloma or by manipulating such B-cells by other techniques to perpetuate a cell line) to produce a monoclonal human antibody-producing cell. See, e.g., U.S. Patent No. 5,916,771; 5,985,615.

TABLE 3 >Rv0002 dnaN DNA polymerase III, b-subunit TB.seq 2052:3257 MW:42114 >emb|AL123456|MTBH37RV:2052-3260, dnaN SEQ ID NO:1

ATGGACGCGGCTACGACAAGAGTTGGCCTCACCGACTTGACGTTTCGTTTGCTACGAGAGTCTT TCGCCGATGCGGTGTCGTGGGTGGCTAAAAATCTGCCAGCCAGGCCCGCGGTGCCGGTGCTCT CCGGCGTGTTGTTGACCGGCTCGGACAACGGTCTGACGATTTCCGGATTCGACTACGAGGTTTC CGCCGAGGCCCAGGTTGGCGCTGAAATTGTTTCTCCTGGAAGCGTTTTAGTTTCTGGCCGATTG TTGTCCGATATTACCCGGGCGTTGCCTAACAAGCCCGTAGACGTTCATGTCGAAGGTAACCGGG

TCGCATTGACCTGCGGTAACGCCAGGTTTTCGCTACCGACGATGCCAGTCGAGGATTATCCGAC GCTGCCGACGCTGCCGGAAGAGACCGGATTGTTGCCTGCGGAATTATTCGCCGAGGCAATCAG TCAGGTCGCTATCGCCGCCGGCCGGGACGACACGTTGCCTATGTTGACCGGCATCCGGGTCGA AATCCTCGGTGAGACGGTGGTTTTGGCCGCTACCGACAGGTTTCGCCTGGCTGTTCGAGAACTG AAGTGGTCGGCGTCGTCGCCAGATATCGAAGCGGCTGTGCTGGTCCCGGCCAAGACGCTGGC

CGAGGCCGCCAAAGCGGGCATCGGCGGCTCTGACGTTCGTTTGTCGTTGGGTACTGGGCCGG GGGTGGGCAAGGATGGCCTGCTCGGTATCAGTGGGAACGGCAAGCGCAGCACCACGCGACTT CTTGATGCCGAGTTCCCGAAGTTTCGGCAGTTGCTACCAACCGAACACACCGCGGTGGCCACC ATGGACGTGGCCGAGTTGATCGAAGCGATCAAGCTGGTTGCGTTGGTAGCTGATCGGGGCGCG CAGGTGCGCATGGAGTTCGCTGATGGCAGCGTGCGGCTTTCTGCGGGTGCCGATGATGTTGGA

CGAGCCGAGGAAGATCTTGTTGTTGACTATGCCGGTGAACCATTGACGATTGCGTTTAACCCAA CCTATCTAACGGACGGTTTGAGTTCGTTGCGCTCGGAGCGAGTGTCTTTCGGGTTTACGACTGC GGGTAAGCCTGCCTTGCTACGTCCGGTGTCCGGGGACGATCGCCCTGTGGCGGGTCTGAATGG CAACGGTCCGTTCCCGGCGGTGTCGACGGACTATGTCTATCTGTTGATGCCGGTTCGGTTGCCG GGCTGA

>Rv0003 recF DNA replication and SOS induction TB.seq 3280:4434 MW:42181 >emb|AL123456|MTBH37RV:3280-4437, recF SEQ ID NO:2

GTGTACGTCCGTCATTTGGGGCTGCGTGACTTCCGGTCCTGGGCATGTGTAGATCTGGAATTGC ATCCAGGGCGGACGGTTTTTGTTGGGCCTAACGGTTATGGTAAGACGAATCTTATTGAGGCACT GTGGTATTCGACGACGTTAGGTTCGCACCGCGTTAGCGCCGATTTGCCGTTGATCCGGGTAGGT ACCGATCGTGCGGTGATCTCCACGATCGTGGTGAACGACGGTAGAGAATGTGCCGTCGACCTC GAGATCGCCACGGGGCGAGTCAACAAAGCGCGATTGAATCGATCATCGGTCCGAAGTACACGT GATGTGGTCGGAGTGCTTCGAGCTGTGTTGTTTGCCCCTGAGGATCTGGGGTTGGTTCGTGGG GATCCCGCTGACCGGCGGCGCTATCTGGATGATCTGGCGATCGTGCGTAGGCCTGCGATCGCT

GCGGTACGAGCCGAATATGAGAGGGTGTTGCGCCAGCGGACGGCGTTATTGAAGTCCGTACCT GGAGCACGGTATCGGGGTGACCGGGGTGTGTTTGACACTCTTGAGGTATGGGACAGTCGTTTG GCGGAGCACGGGGCTGAACTGGTGGCCGCCCGCATCGATTTGGTCAACCAGTTGGCACCGGA AGTGAAGAAGGCATACCAGCTGTTGGCGCCGGAATCGCGATCGGCGTCTATCGGTTATCGGGC CAGCATGGATGTAACCGGTCCCAGCGAGCAGTCAGATATCGATCGGCAATTGTTAGCAGCTCGG

CTGTTGGCGGCGCTGGCGGCCCGTCGGGATGCCGAACTCGAGCGTGGGGTTTGTCTAGTTGGT CCGCACCGTGACGACCTAATACTGCGACTAGGCGATCAACCCGCGAAAGGATTTGCTAGCCATG GGGAGGCGTGGTCGTTGGCGGTGGCACTGCGGTTGGCGGCCTATCAACTGTTACGCGTTGATG GTGGTGAGCCGGTGTTGTTGCTCGACGACGTGTTCGCCGAACTGGATGTCATGCGCCGTCGAG CGTTGGCGACGGCGGCCGAGTCCGCCGAACAGGTGTTGGTGACTGCCGCGGTGCTCGAGGAT

ATTCCCGCCGGCTGGGACGCCAGGCGGGTGCACATCGATGTGCGTGCCGATGACACCGGATC GATGTCGGTGGTTCTGCCATGA

>Rv0005 gyrB DNA gyrase subunit B TB.seq .5123:7264 MW:78441 >emb|AL123456|MTBH37RV:5123-7267, gyrB SEQ ID N0:3

ATGGGTAAAAACGAGGCCAGAAGATCGGCCCTGGCGCCCGATCACGGTACAGTGGTGTGCGAC CCCCTGCGGCGACTCAACCGCATGCACGCAACCCCTGAGGAGAGTATTCGGATCGTGGCTGCC CAGAAAAAGAAGGCCCAAGACGAATACGGCGCTGCGTCTATCACCATTCTCGAAGGGCTGGAG GCCGTCCGCAAACGTCCCGGCATGTACATTGGCTCGACCGGTGAGCGCGGTTTACACCATCTC ATTTGGGAGGTGGTCGACAACGCGGTCGACGAGGCGATGGCCGGTTATGCAACCACAGTGAAC

GTAGTGCTGCTTGAGGATGGCGGTGTCGAGGTCGCCGACGACGGCCGCGGCATTCCGGTCGC CACCCACGCCTCCGGCATACCGACCGTCGACGTGGTGATGACACAACTACATGCCGGCGGCAA GTTCGACTCGGACGCGTATGCGATATCTGGTGGTCTGCACGGCGTCGGCGTGTCGGTGGTTAA CGCGCTATCCACCCGGCTCGAAGTCGAGATCAAGCGCGACGGGTACGAGTGGTCTCAGGTTTA TGAGAAGTCGGAACCCCTGGGCCTCAAGCAAGGGGCGCCGACCAAGAAGACGGGGTCAACGG

TGCGGTTCTGGGCCGACCCCGCTGTTTTCGAAACCACGGAATACGACTTCGAAACCGTCGCCC GCCGGCTGCAAGAGATGGCGTTCCTCAACAAGGGGCTGACCATCAACCTGACCGACGAGAGGG TGACCCAAGACGAGGTCGTCGACGAAGTGGTCAGCGACGTCGCCGAGGCGCCGAAGTCGGCA AGTGAACGCGCAGCCGAATCCACTGCACCGCACAAAGTTAAGAGCCGCACCTTTCACTATCCGG GTGGCCTGGTGGACTTCGTGAAACACATCAACCGCACCAAGAACGCGATTCATAGCAGCATCGT

GGACTTTTCCGGCAAGGGCACCGGGCACGAGGTGGAGATCGCGATGCAATGGAACGCCGGGT ATTCGGAGTCGGTGCACACCTTCGCCAACACCATCAACACCCACGAGGGCGGCACCCACGAAG AGGGCTTCCGCAGCGCGCTGACGTCGGTGGTGAACAAGTACGCCAAGGACCGCAAGCTACTGA AGGACAAGGACCCCAACCTCACCGGTGACGATATCCGGGAAGGCCTGGCCGCTGTGATCTCGG TGAAGGTCAGCGAACCGCAGTTCGAGGGCCAGACCAAGACCAAGTTGGGCAACACCGAGGTCA AATCGTTTGTGCAGAAGGTCTGTAACGAACAGCTGACCCACTGGTTTGAAGCCAACCCCACCGA CGCGAAAGTCGTTGTGAACAAGGCTGTGTCCTCGGCGCAAGCCCGTATCGCGGCACGTAAGGC

ACGAGAGTTGGTGCGGCGTAAGAGCGCCACCGACATCGGTGGATTGCCCGGCAAGCTGGCCG ATTGCCGTTCCACGGATCCGCGCAAGTCCGAACTGTATGTCGTAGAAGGTGACTCGGCCGGCG GTTCTGCAAAAAGCGGTCGCGATTCGATGTTCCAGGCGATACTTCCGCTGCGCGGCAAGATCAT CAATGTGGAGAAAGCGCGCATCGACCGGGTGCTAAAGAACACCGAAGTTCAGGCGATCATCAC GGCGCTGGGCACCGGGATCCACGACGAGTTCGATATCGGCAAGCTGCGCTACCACAAGATCGT

GCTGATGGCCGACGCCGATGTTGACGGCCAACATATTTCCACGCTGTTGTTGACGTTGTTGTTC CGGTTCATGCGGCCGCTCATCGAGAACGGGCATGTGTTTTTGGCACAACCGCCGCTGTACAAAC TCAAGTGGCAGCGCAGTGACCCGGAATTCGCATACTCCGACCGCGAGCGCGACGGTCTGCTGG AGGCGGGGCTGAAGGCCGGGAAGAAGATCAACAAGGAAGACGGCATTCAGCGGTACAAGGGT CTAGGTGAAATGGACGCTAAGGAGTTGTGGGAGACCACCATGGATCCCTCGGTTCGTGTGTTGC

GTCAAGTGACGCTGGACGACGCCGCCGCCGCCGACGAGTTGTTCTCCATCCTGATGGGCGAGG ACGTCGACGCGCGGCGCAGCTTTATCACCCGCAACGCCAAGGATGTTCGGTTCCTGGATGTCTA A

>Rv0006 gyrA DNA gyrase subunit A TB.seq 7302:9815 MW:92276

>emb|AL123456|MTBH37RV:7302-9818, gyrA SEQ ID N0:4

ATGACAGACACGACGTTGCCGCCTGACGACTCGCTCGACCGGATCGAACCGGTTGACATCGAG CAGGAGATGCAGCGCAGCTACATCGACTATGCGATGAGCGTGATCGTCGGCCGCGCGCTGCCG GAGGTGCGCGACGGGCTCAAGCCCGTGCATCGCCGGGTGCTCTATGCAATGTTCGATTCCGGC TTCCGCCCGGACCGCAGCCACGCCAAGTCGGCCCGGTCGGTTGCCGAGACCATGGGCAACTA

CCACCCGCACGGCGACGCGTCGATCTACGACAGCCTGGTGCGCATGGCCCAGCCCTGGTCGC TGCGCTACCCGCTGGTGGACGGCCAGGGCAACTTCGGCTCGCCAGGCAATGACCCACCGGCG GCGATGAGGTACACCGAAGCCCGGCTGACCCCGTTGGCGATGGAGATGCTGAGGGAAATCGAC GAGGAGACAGTCGATTTCATCCCTAACTACGACGGCCGGGTGCAAGAGCCGACGGTGCTACCC AGCCGGTTCCCCAACCTGCTGGCCAACGGGTCAGGCGGCATCGCGGTCGGCATGGCAACCAAT

ATCCCGCCGCACAACCTGCGTGAGCTGGCCGACGCGGTGTTCTGGGCGCTGGAGAATCACGAC GCCGACGAAGAGGAGACCCTGGCCGCGGTCATGGGGCGGGTTAAAGGCCCGGACTTCCCGAC CGCCGGACTGATCGTCGGATCCCAGGGCACCGCTGATGCCTACAAAACTGGCCGCGGCTCCAT TCGAATGCGCGGAGTTGTTGAGGTAGAAGAGGATTCCCGCGGTCGTACCTCGCTGGTGATCAC CGAGTTGCCGTATCAGGTCAACCACGACAACTTCATCACTTCGATCGCCGAACAGGTCCGAGAC

GGCAAGCTGGCCGGCATTTCCAACATTGAGGACCAGTCTAGCGATCGGGTCGGTTTACGCATC GTCATCGAGATCAAGCGCGATGCGGTGGCCAAGGTGGTGATCAATAACCTTTACAAGCACACCC AGCTGCAGACCAGCTTTGGCGCCAACATGCTAGCGATCGTCGACGGGGTGCCGCGCACGCTGC GGCTGGACCAGCTGATCCGCTATTACGTTGACCACCAACTCGACGTCATTGTGCGGCGCACCAC CTACCGGCTGCGCAAGGCAAACGAGCGAGCCCACATTCTGCGCGGCCTGGTTAAAGCGCTCGA CGCGCTGGACGAGGTCATTGCACTGATCCGGGCGTCGGAGACCGTCGATATCGCCCGGGCCG GACTGATCGAGCTGCTCGACATCGACGAGATCCAGGCCCAGGCAATCCTGGACATGCAGTTGC

GGCGCCTGGCCGCACTGGAACGCCAGCGCATCATCGACGACCTGGCCAAAATCGAGGCCGAG ATCGCCGATCTGGAAGACATCCTGGCAAAACCCGAGCGGCAGCGTGGGATCGTGCGCGACGAA CTCGCCGAAATCGTGGACAGGCACGGCGACGACCGGCGTACCCGGATCATCGCGGCCGACGG AGACGTCAGCGACGAGGATTTGATCGCCCGCGAGGACGTCGTTGTCACTATCACCGAAACGGG ATACGCCAAGCGCACCAAGACCGATCTGTATCGCAGCCAGAAACGCGGCGGCAAGGGCGTGCA

GGGTGCGGGGTTGAAGCAGGACGACATCGTCGCGCACTTCTTCGTGTGCTCCACCCACGATTT GATCCTGTTCTTCACCACCCAGGGACGGGTTTATCGGGCCAAGGCCTACGACTTGCCCGAGGC CTCCCGGACGGCGCGCGGGCAGCACGTGGCCAACCTGTTAGCCTTCCAGCCCGAGGAACGCA TCGCCCAGGTCATCCAGATTCGCGGCTACACCGACGCCCCGTACCTGGTGCTGGCCACTCGCA ACGGGCTGGTGAAAAAGTCCAAGCTGACCGACTTCGACTCCAATCGCTCGGGCGGAATCGTGG

CGGTCAACCTGCGCGACAACGACGAGCTGGTCGGTGCGGTGCTGTGTTCGGCCGGCGACGAC CTGCTGCTGGTCTCGGCCAACGGGCAGTCCATCAGGTTCTCGGCGACCGACGAGGCGCTGCG GCCAATGGGTCGTGCCACCTCGGGTGTGCAGGGCATGCGGTTCAATATCGACGACCGGCTGCT GTCGCTGAACGTCGTGCGTGAAGGCACCTATCTGCTGGTGGCGACGTCAGGGGGCTATGCGAA ACGTACCGCGATCGAGGAATACCCGGTACAGGGCCGCGGCGGTAAAGGTGTGCTGACGGTCAT

GTACGACCGCCGGCGCGGCAGGTTGGTTGGGGCGTTGATTGTCGACGACGACAGCGAGCTGT ATGCCGTCACTTCCGGCGGTGGCGTGATCCGCACCGCGGCACGCCAGGTTCGCAAGGCGGGA CGGCAGACCAAGGGTGTTCGGTTGATGAATCTGGGCGAGGGCGACACACTGTTGGCCATCGCG CGCAACGCCGAAGAAAGTGGCGACGATAATGCCGTGGACGCCAACGGCGCAGACCAGACGGG CAATTAA

>Rv0014c pknB serine-threonine protein kinase TB.seq 15593:17470 MW:66511 >emb|AL123456|MTBH37RV:d 7470-15590, pknB SEQ ID NO:5 ATGACCACCCCTTCCCACCTGTCCGACCGCTACGAACTTGGCGAAATCCTTGGATTTGGGGGCA TGTCCGAGGTCCACCTGGCCCGCGACCTCCGGTTGCACCGCGACGTTGCGGTCAAGGTGCTGC

GCGCTGATCTAGCCCGCGATCCCAGTTTTTACCTTCGCTTCCGGCGTGAGGCGCAAAACGCCG CGGCATTGAACCACCCTGCAATCGTCGCGGTCTACGACACCGGTGAAGCCGAAACGCCCGCCG GGCCATTGCCCTACATCGTCATGGAATACGTCGACGGCGTTACCCTGCGCGACATTGTCCACAC CGAAGGGCCGATGACGCCCAAACGCGCCATCGAGGTCATCGCCGACGCCTGCCAAGCGCTGA ACTTCAGTCATCAGAACGGAATCATCCACCGTGACGTCAAGCCGGCGAACATCATGATCAGCGC

GACCAATGCAGTAAAGGTGATGGATTTCGGCATCGCCCGCGCCATTGCCGACAGCGGCAACAG CGTGACCCAGACCGCAGCAGTGATCGGCACGGCGCAGTACCTGTCACCCGAACAGGCCCGGG GTGATTCCGTCGACGCCCGATCCGATGTCTATTCCTTGGGCTGTGTTCTTTATGAAGTCCTCACC GGGGAGCCACCTTTCACCGGCGACTCACCCGTCTCGGTTGCCTACCAACATGTGCGCGAAGAC CCGATCCCACCTTCGGCGCGGCACGAAGGCCTCTCCGCCGACCTGGACGCCGTCGTTCTCAAG GCGCTGGCCAAAAATCCGGAAAACCGCTATCAGACAGCGGCGGAGATGCGCGCCGACCTGGTC CGCGTGCACAACGGTGAGCCGCCCGAGGCGCCCAAAGTGCTCACCGATGCCGAGCGGACCTC

GCTGCTGTCGTCTGCGGCCGGCAACCTTAGCGGTCCGCGCACCGATCCGCTACCACGCCAGGA CTTAGACGACACCGACCGTGACCGCAGCATCGGTTCGGTGGGCCGTTGGGTTGCGGTGGTCGC CGTGCTCGCTGTGCTGACCGTCGTGGTAACCATCGCCATCAACACGTTCGGCGGCATCACCCG CGACGTTCAAGTTCCCGACGTTCGGGGTCAATCCTCCGCCGACGCCATCGCCACACTGCAAAA CCGGGGCTTCAAAATCCGCACCTTGCAG AAGCCGGACTCGACAATCCCACCGGACCACGTTAT

CGGCACCGACCCGGCCGCCAACACGTCGGTGAGTGCAGGCGACGAGATCACAGTCAACGTGT CCACCGGACCCGAGCAACGCGAAATACCCGACGTCTCCACGCTGACATACGCCGAAGCGGTCA AGAAACTGACTGCCGCCGGATTCGGCCGCTTCAAGCAAGCGAATTCGCCGTCCACCCCGGAAC TGGTGGGCAAGGTCATCGGGACCAACCCGCCAGCCAACCAGACGTCGGCCATCACCAATGTGG TCATCATCATCGTTGGCTCTGGTCCGGCGACCAAAGACATTCCCGATGTCGCGGGCCAGACCGT

CGACGTGGCGCAGAAGAACCTCAACGTCTACGGCTTCACCAAATTCAGTCAGGCCTCGGTGGA CAGCCCCCGTCCCGCCGGCGAGGTGACCGGCACCAATCCACCCGCAGGCACCACAGTTCCGG TCGATTCAGTCATCGAACTACAGGTGTCCAAGGGCAACCAATTCGTCATGCCCGACCTATCCGG CATGTTCTGGGTCGACGCCGAACCACGATTGCGCGCGCTGGGCTGGACCGGGATGCTCGACAA AGGGGCCGACGTCGACGCCGGTGGCTCCCAACACAACCGGGTCGTCTATCAAAACCCGCCGG

CGGGGACCGGCGTCAACCGGGACGGCATCATCACGCTGAGGTTCGGCCAGTAG

>Rv0016c pbpA TB.seq 18762:20234 MW:51577 >emb|AL123456|MTBH37RV:c20234-18759, pbpA SEQ ID NO:6 ATGAACGCCTCTCTGCGCCGAATATCGGTGACCGTGATGGCGTTGATCGTGTTGCTACTGCTCA

ACGCGACCATGACGCAGGTCTTCACCGCCGACGGGCTGCGTGCCGATCCCCGCAACCAGCGA GTGTTGCTCGACGAGTATTCACGGCAGCGCGGCCAGATCACCGCTGGTGGCCAACTGCTGGCG TACTCGGTAGCCACCGACGGCCGCTTTCGTTTCCTGCGGGTCTATCCCAATCCTGAGGTGTACG CGCCGGTTACCGGCTTCTACTCCCTGCGCTATTCCAGCACCGCCCTAGAACGAGCCGAGGACC CGATATTGAACGGGTCCGACCGCCGTCTGTTCGGCCGCCGGCTGGCCGACTTCTTCACCGGTC

GCGACCCACGCGGCGGTAATGTCGATACCACGATCAACCCGCGCATTCAGCAAGCCGGCTGGG ACGCGATGCAGCAAGGCTGCTACGGGCCCTGTAAGGGAGCGGTGGTCGCCCTTGAGCCATCAA CCGGCAAGATTTTGGCGTTGGTGTCTTCTCCGTCCTACGACCCCAACCTGCTGGCGTCGCATAA CCCCGAGGTGCAGGCGCAAGCCTGGCAGCGGCTTGGCGACAATCCCGCCTCTCCACTGACCAA CCGTGCCATCTCTGAGACGTATCCACCGGGTTCGACTTTCAAAGTGATCACCACTGCGGCCGCG

CTGGCCGCCGGGGCCACCGAGACCGAACAGCTGACTGCGGCGCCCACAATTCCGTTGCCAGG CAGCACCGCCCAGCTAGAGAACTACGGCGGTGCGCCGTGCGGGGACGAACCCACCGTGTCGC TGCGTGAGGCATTCGTCAAATCATGCAACACCGCATTCGTCCAGCTGGGCATCCGCACCGGCG CCGACGCCCTGCGCAGCATGGCGCGCGCGTTCGGTCTCGATAGCCCACCGCGCCCAACTCCG CTGCAAGTGGCGGAATCAACCGTCGGGCCTATCCCGGACAGCGCCGCACTAGGGATGACCAGT ATCGGCCAAAAGGACGTTGCGCTGACCCCGCTAGCGAACGCAGAAATAGCCGCGACCATCGCA AACGGCGGCATTACGATGAGGCCTTATCTAGTCGGCAGCCTCAAGGGACCGGACCTAGCCAAT

ATCTCAACCACCGTCGGATACCAGCAGCGCCGCGCGGTGTCACCGCAGGTCGCCGCTAAGCTA ACAGAGCTGATGGTCGGCGCCGAGAAAGTCGCACAGCAGAAAGGGGCAATCCCCGGCGTGCA GATCGCATCCAAGACGGGCACCGCCGAACATGGCACCGACCCTCGTCACACTCCACCGCACGC TTGGTACATCGCCTTTGCGCCCGCACAAGCGCCCAAGGTGGCTGTTGCCGTGCTGGTGGAGAA CGGGGCTGATCGGCTGTCCGCCACCGGAGGTGCCCTCGCGGCACCGATCGGGCGGGCGGTG

ATCGAAGCCGCACTGCAGGGGGAACCATGA

>Rv0017c rodA TB.seq 20234:21640 MW:50612 >emb|AL123456|MTBH37RV:c21640-20231 , rodA SEQ ID NO:7 ATGACGACACGACTGCAAGCGCCGGTGGCCGTAACGCCCCCGTTGCCGACTCGGCGCAACGC

TGAACTGCTGCTGCTGTGCTTTGCCGCCGTAATCACGTTTGCCGCACTGCTGGTCGTGCAGGCC AATCAAGACCAGGGGGTGCCCTGGGACTTGACTAGCTACGGACTGGCCTTCCTGACCCTGTTC GGATCCGCGCATCTGGCCATCCGGCGCTTCGCCCCCTACACTGACCCGCTGTTGCTCCCGGTG GTGGCACTGCTCAACGGACTTGGCCTGGTAATGATCCACCGCCTCGATCTGGTGGACAACGAG ATCGGCGAGCATCGGCACCCCAGCGCAAACCAGCAGATGCTGTGGACGCTGGTGGGCGTAGC

TGCCTTCGCGCTCGTGGTGACCTTCCTCAAGGACCACCGACAGCTCGCACGCTACGGCTACATT TGCGGGCTCGCGGGTCTGGTTTTCTTGGCAGTTCCCGCGCTGCTCCCGGCAGCACTGTCCGAA CAGAACGGCGCCAAGATCTGGATCCGGTTGCCCGGCTTCTCGATTCAACCCGCCGAATTTTCAA AGATTCTGCTGCTGATCTTCTTTTCGGCGGTACTGGTGGCCAAACGCGGCCTGTTCACCAGCGC CGGCAAACATTTGCTCGGAATGACCCTGCCGCGCCCGCGAGACCTCGCGCCACTGTTGGCAGC

CTGGGTCATCTCGGTGGGTGTGATGGTCTTCGAGAAAGACCTCGGCGCTTCGCTGCTGCTGTAC ACATCGTTTCTGGTGGTGGTTTACCTCGCCACCCAGCGGTTCAGTTGGGTCGTCATCGGCCTGA CTCTGTTCGCGGCAGGAACCTTGGTGGCGTACTTCATTTTTGAGCACGTCCGGCTCCGCGTACA GACCTGGCTGGATCCGTTCGCAGATCCAGACGGCACCGGATATCAGATCGTGCAGTCGCTTTTC AGCTTCGCTACAGGCGGTATCTTCGGCACCGGGCTCGGTAATGGTCAACCCGACACCGTGCCC

GCGGCATCCACCGATTTCATCATCGCCGCGTTCGGCGAAGAGCTTGGGTTGGTGGGCTTGACG GCCATCCTGATGCTCTACACCATCGTGATCATCCGGGGTTTGCGCACGGCCATCGCCACCCGC GATAGCTTCGGCAAGCTGCTGGCCGCCGGCCTCTCATCGACGCTAGCCATTCAGCTGTTCATCG TCGTCGGCGGTGTGACCCGACTCATTCCGCTGACCGGGTTGACCACACCGTGGATGTCCTACG GCGGGTCTTCACTGCTGGCCAACTACATATTGCTGGCCATCCTGGCACGCATCTCGCACGGAGC

CCGCCGCCCACTGCGCACCCGCCCACGAAATAAGTCGCCGATTACGGCGGCCGGCACCGAGG TCATCGAACGCGTATGA >Rv0018c ppp TB.seq 21640:23181 MW:53781 >emb|AL123456|MTBH37RV:c23181-21637, ppp SEQ ID N0:8

GTGGCGCGCGTGACCCTGGTCCTGCGATACGCGGCGCGCAGCGATCGCGGCTTGGTACGCGC CAACAACGAAGACTCGGTCTACGCTGGGGCACGGCTATTGGCCCTGGCCGACGGCATGGGTG

GGCATGCGGCCGGCGAGGTGGCGTCCCAGTTGGTGATTGCCGCATTGGCCCATCTCGATGACG ACGAGCCCGGTGGCGATCTGCTGGCCAAGCTGGATGCCGCGGTGCGCGCCGGCAACTCGGCT ATCGCAGCGCAAGTCGAGATGGAGCCCGATCTCGAAGGCATGGGTACCACGCTCACCGCAATC CTGTTCGCGGGCAACCGGCTCGGCCTGGTGCATATCGGTGACTCGCGCGGTTACCTGCTGCGC G ACGGTG AGCTGACGCAGATCACCAAGGACGACACGTTTGTCCAAACGCTGGTCGACGAAGGC

CGGATCACCCCGGAGGAGGCGCACAGCCACCCGCAACGCTCGTTGATCATGCGGGCGTTGAC CGGCCATGAGGTCGAACCGACGCTGACCATGCGAGAAGCCCGCGCCGGTGATCGTTACCTGCT GTGCTCGGACGGGTTGTCCGATCCGGTTAGCGATGAAACTATCCTCGAGGCCCTGCAGATCCC CGAGGTTGCCGAGAGCGCTCACCGCCTCATTGAACTGGCGCTGCGCGGCGGCGGCCCCGACA ACGTCACTGTCGTCGTCGCCGACGTCGTCGACTACGACTACGGCCAGACCCAACCGATTCTGG

CCGGGGCGGTCTCAGGCGACGACGACCAACTGACCCTGCCCAACACCGCCGCCGGCCGGGCC TCTGCCATCAGCCAGCGCAAGGAGATCGTTAAACGCGTTCCGCCACAGGCCGATACATTCAGTC GGCCACGGTGGTCGGGCCGACGGCTAGCATTCGTTGTCGCACTGGTGACCGTGCTGATGACTG CGGGCCTGCTCATTGGTCGCGCGATCATCCGCAGCAACTACTACGTAGCGGACTACGCCGGCA GCGTGTCCATCATGCGGGGGATTCAAGGGTCGCTACTGGGCATGTCCCTGCACCAGCCTTACC

TGATGGGCTGCCTCAGCCCGCGTAACGAGCTGTCGCAGATCAGCTACGGACAGTCTGGGGGCC CTCTCGACTGCCATCTGATGAAACTGGAGGATCTGCGACCGCCGGAGCGCGCACAGGTTCGGG CCGGTCTCCCGGCCGGCACTCTCGATGACGCCATCGGGCAGTTGCGCGAACTGGCGGCCAACT CCCTGCTGCCGCCTTGCCCGGCGCCGCGTGCCACGTCCCCGCCCGGGCGCCCGGCCCCACCC ACCACCAGCGAGACAACCGAACCAAACGTCACCTCCTCGCCAGCCTCTCCATCACCCACCACCT

CCGCGCCGGCCCCCACCGGAACTACTCCTGCCATCCCCACGAGTGCCTCCCCGGCAGCGCCC GCGTCGCCGCCGACGCCTTGGCCCGTCACCAGCTCGCCGACGATGGCCGCACTTCCGCCACC CCCGCCTCAGCCGGGCATCGACTGCCGGGCGGCGGCATGA

>Rv0019c - TB.seq 23273:23737 MW:17153

>emb|AL123456|MTBH37RV:c23737-23270, Rv0019c SEQ ID N0:9

ATGCAGGGGTTGGTACTGCAACTGACGCGTGCCGGATTCTTGATGTTGTTGTGGGTATTCATCT GGTCCGTGCTACGGATCTTGAAGACCGACATTTATGCGCCGACCGGCGCGGTCATGATGCGCC GCGGCCTGGCGCTGCGAGGGACGCTCTTAGGCGCGCGTCAGCGCCGGCACGCTGCACGCTAC CTGGTGGTGACCGAAGGTGCGTTGACTGGCGCGCGTATCACGCTGAGCGAACAGCCGGTGTTG

ATCGGGCGCGCCGACGACTCGACCCTGGTGCTGACCGACGACTACGCCTCGACGCGGCACGC TCGGCTGTCTATGCGCGGCTCCGAGTGGTACGTCGAAGATCTAGGATCGACCAACGGCACTTA CCTGGACAGGGCGAAGGTGACGACTGCGGTACGAGTTCCGATCGGAACGCCGGTTCGCATCG GCAAAACTGCAATCGAGTTGCGCCCGTGA

>Rv0020c - TB.seq 23864:25444 MW:56881 >emb|AL123456|MTBH37RV:c25444-23861 , Rv0020c SEQ ID NO:10

ATGGGTAGCCAGAAAAGGCTGGTTCAGCGCGTTGAGCGCAAACTCGAGCAGACGGTTGGCGAT GCGTTTGCCCGCATCTTTGGAGGCTCGATCGTCCCGCAAGAGGTCGAAGCCCTGCTGCGCCGC GAGGCGGCCGACGGCATCCAGTCGCTGCAGGGAAATCGCCTTTTGGCGCCCAACGAATACATC ATTACCCTCGGTGTGCACGACTTTGAGAAGTTGGGCGCTGATCCTGAGCTGAAGTCAACCGGTT TTGCTCGGGACTTGGCGGACTATATCCAAGAACAGGGGTGGCAAACGTATGGTGATGTGGTCGT

CCGATTCGAGCAGTCGTCGAACCTGCATACCGGCCAGTTCCGCGCCCGCGGCACTGTTAACCC CGACGTTGAGACCCACCCGCCGGTCATCGATTGCGCCCGGCCACAATCAAACCACGCGTTTGG CGCAGAACCAGGAGTAGCACCAATGAGTGACAATTCGAGCTACCGTGGCGGTCAGGGGCAGGG GCGTCCCGACGAGTATTACGACGACCGCTATGCGCGTCCGCAAGAGGATCCGCGTGGTGGCCC GGATCCGCAAGGCGGATCTGACCCCCGCGGGGGGTATCCACCCGAGACGGGCGGCTACCCGC

CCCAGCCGGGCTACCCACGCCCGCGCCACCCGGACCAGGGCGACTACCCCGAGCAAATCGGG TACCCCGACCAGGGCGGTTACCCCGAGCAACGCGGTTACCCCGAGCAACGCGGCTACCCCGA CCAGCGCGGGTACCAGGACCAGGGTCGAGGCTACCCCGACCAAGGGCAGGGGGGCTATCCGC CGCCCTACGAGCAACGCCCTCCTGTTTCTCCCGGCCCGGCTGCCGGCTACGGCGCTCCCGGCT ACGACCAGGGCTATCGCCAAAGCGGCGGCTACGGCCCTTCACCCGGTGGCGGCCAGCCCGGC

TACGGCGGGTACGGGGAGTACGGGCGTGGCCCGGCTCGCCACGAGGAGGGCAGCTATGTGCC CTCTGGCCCTCCGGGCCCGCCCGAGCAACGACCGGCTTACCCCGACCAAGGCGGTTACGACC AGGGCTACCAGCAAGGCGCCACGACATACGGCCGGCAAGACTATGGCGGCGGCGCTGACTAC ACCCGCTACACCGAATCCCCGCGGGTCCCGGGATACGCTCCTCAGGGTGGCGGGTACGCCGA ACCCGCCGGCCGAGACTACGACTACGGCCAATCAGGCGCTCCGGACTACGGTCAGCCAGCGC

CCGGTGGCTACAGCGGTTACGGGCAGGGCGGCTATGGGTCCGCCGGAACGTCGGTTACGCTG CAGCTCGACGACGGCAGCGGACGCACTTACCAGCTCCGCGAGGGCTCCAACATCATCGGTCGC GGACAGGACGCCCAGTTCCGGCTGCCCGACACCGGTGTGTCACGCCGTCACTTGGAGATCCG GTGGGACGGGCAGGTCGCATTGCTCGCAGACCTGAACTCCACCAACGGCACCACTGTTAACAA TGCACCGGTACAGGAGTGGCAGTTGGCCGACGGTGATGTGATCCGCTTGGGACACTCCGAGAT

CATCGTCCGCATGCACTGA

>Rv0032 bioF2 C-terminal similar to B. subtilis BioF TB.seq 34295:36607 MW:86245 >emb|AL123456|MTBH37RV:34295-36610, bioF2 SEQ ID NO:11 ATGCCCACTGGCTTGGGCTATGACTTTCTGCGCCCTGTCGAGGACTCGGGGATCAACGACCTGA

AGCACTATTACTTCATGGCGGATTTGGCCGATGGGCAACCGCTAGGCCGGGCAAACCTCTATAG CGTCTGTTTCGACCTGGCCACCACCGACCGCAAGCTCACTCCGGCCTGGCGAACGACCATCAA ACGGTGGTTTCCGGGGTTTATGACCTTCCGTTTCCTCGAGTGCGGGTTGCTCACCATGGTGAGC AACCCGCTGGCGTTGCGGTCCGACACCGACTTGGAGCGGGTATTGCCTGTGCTGGCCGGCCAG ATGGACCAGTTGGCGCATGACGACGGGTCGGATTTCTTGATGATCCGGGACGTGGACCCGGAA CACTACCAGCGATACCTTGACATCCTGCGCCCGTTGGGCTTTCGGCCTGCGCTGGGCTTTTCCC GGGTAGACACGACCATCAGCTGGTCGAGCGTGGAAGAGGCACTGGGCTGCCTGTCTCACAAAA

GGCGCCTGCCGTTGAAGACGTCGCTGGAGTTTCGTGAGCGGTTCGGTATCGAGGTCGAGGAAC TCGACGAGTATGCCGAGCATGCGCCGGTATTGGCCCGGCTTTGGCGCAACGTCAAGACGGAGG CAAAGGATTACCAGCGCGAGGACCTGAACCCTGAGTTCTTCGCGGCGTGTTCTCGGCATCTGCA TGGACGTAGCAGACTGTGGTTGTTCCGCTACCAGGGCACGCCAATTGCCTTCTTTTTGAACGTTT GGGGTGCGGATGAGAACTACATACTGCTTGAGTGGGGCATCGATCGTGATTTTGAACATTATAG

GAAGGCGAATCTGTACCGGGCGGCGCTGATGCTCAGCCTAAAAGATGCGATCAGCCGAGATAA ACGGCGAATGGAAATGGGTATTACGAACTATTTCACAAAACTTCGCATTCCGGGTGCCCGAGTC ATACCGACCATCTATTTCCTGCGTCACAGCACGGATCCGGTGCATACGGCAACGTTAGCGCGAA TGATGATGCACAATATTCAACGGCCAACGCTACCCGACGATATGTCGGAGGAATTCTGTCGCTG GGAAGAGCGAATACGTCTGGACCAGGACGGGCTACCCGAACACGATATCTTTCGCAAGATCGAT

CGTCAGCACAAATACACGGGGCTCAAACTCGGCGGAGTCTACGGTTTTTATCCCCGATTCACCG GACCGCAGCGATCCACGGTCAAGGCCGCGGAGCTGGGCGAGATCGTGTTGCTGGGCACGAAC TCGTATCTGGGCCTGGCCACCCATCCAGAGGTGGTGGAGGCCTCGGCGGAGGCCACGCGACG GTACGGCACCGGCTGCTCGGGTTCGCCGTTGCTGAACGGCACGTTGGACTTGCACGTCTCGCT TGAGCAGGAACTAGCCTGTTTTTTGGGCAAACCCGCCGCCGTGTTGTGCTCCACCGGATATCAG

AGCAACCTGGCGGCGATCAGCGCGCTATGCGAATCCGGGGACATGATCATCCAAGACGCGCTG AACCACCGCAGCCTGTTCGACGCCGCCAGGTTGTCCGGGGCCGACTTCACCTTGTACCGGCAC AACGACATGGACCACCTGGCGCGGGTGCTACGCCGCACCGAGGGGCGCCGCCGGATCATCGT CGTGGACGCGGTGTTCAGCATGGAAGGCACCGTCGCCGACCTGGCCACCATCGCCGAGCTTG CCGACCGGCACGGCTGCCGGGTCTATGTGGACGAGTCCCATGCGCTGGGCGTGCTCGGCCCC

GACGGGCGAGGAGCTTCGGCCGCGTTGGGTGTCTTGGCGCGCATGGACGTGGTGATGGGCAC GTTCAGCAAATCCTTTGCCTCCGTCGGCGGGTTCATCGCCGGAGATCGGCCCGTCGTGGACTA CATCCGGCACAACGGTTCAGGTCATGTGTTTTCCGCCAGCCTGCCGCCGGCCGCCGCGGCTGC CACCCACGCGGCTCTGCGCGTCAGTCGGCGTGAACCCGACCGGCGGGCTCGGGTGCTGGCCG CGGCCGAGTACATGGCCACCGGCCTGGCACGGCAGGGCTATCAGGCCGAGTATCACGGAACC

GCGATCGTGCCGGTGATCCTGGGCAACCCGACCGTGGCGCATGCGGGCTATCTGCGGCTGAT GCGCTCCGGGGTGTATGTGAACCCGGTGGCCCCCCCAGCCGTGCCGGAGGAGCGTTCGGGAT TCCGCACCAGCTACCTAGCCGACCACCGACAATCTGACCTCGACCGGGCCTTGCACGTGTTTGC CGGCCTTGCCGAGGACCTGACCCCGCAAGGAGCCGCGCTATGA

>Rv0050 ponA1 TB.seq 53661 :55694 MW:71119 >emb|AL123456|MTBH37RV:53661 -55697, ponA SEQ ID NO:12 GTGGTGATCCTGTTGCCGATGGTCACCTTCACGATGGCCTACCTGATCGTCGACGTTCCCAAGC CAGGTGACATCCGTACCAACCAGGTCTCCACGATCCTTGCCAGCGACGGCTCGGAAATCGCCA AAATTGTTCCGCCCGAAGGTAATCGGGTCGACGTCAACCTCAGCCAGGTGCCGATGCATGTGC GCCAGGCGGTGATTGCGGCCGAAGACCGCAATTTCTATTCGAATCCGGGATTCTCGTTCACCGG CTTCGCGCGGGCAGTCAAGAACAACCTGTTCGGCGGCGATCTGCAGGGCGGATCGACGATTAC

CCAGCAGTACGTCAAGAACGCGCTGGTCGGTTCCGCACAGCACGGGTGGAGCGGTCTGATGC GCAAGGCGAAAGAATTGGTCATCGCGACGAAGATGTCGGGGGAGTGGTCTAAAGACGATGTGC TGCAGGCGTATCTGAACATCATCTACTTCGGCCGGGGCGCCTACGGCATTTCGGCGGCGTCCA AGGCTTATTTCGACAAGCCCGTCGAGCAGCTGACCGTTGCCGAAGGGGCGTTGTTGGCAGCGC TGATTCGGCGGCCTTCGACGCTGGACCCGGCGGTCGACCCCGAAGGGGCCCATGCCCGCTGG

AATTGGGTACTCGACGGCATGGTGGAAACCAAGGCTCTCTCGCCGAATGACCGTGCGGCGCAG GTGTTTCCCGAGACAGTGCCGCCCGATCTGGCCCGGGCAGAGAATCAGACCAAAGGACCCAAC GGGCTGATCGAGCGGCAGGTGACAAGGGAGTTGCTCGAGCTGTTCAACATCGACGAGCAGACC CTCAACACCCAGGGGCTGGTGGTCACCACCACGATTGATCCGCAGGCCCAACGGGCGGCGGA GAAGGCGGTTGCGAAATACCTGGACGGGCAGGACCCCGACATGCGTGCCGCCGTGGTTTCCAT

CGACCCGCACAACGGGGCGGTGCGTGCGTACTACGGTGGCGACAATGCCAATGGCTTTGACTT CGCTCAAGCGGGATTGCAGACTGGATCGTCGTTTAAGGTGTTTGCTCTGGTGGCCGCCCTTGAG CAGGGGATCGGCCTGGGCTACCAGGTAGACAGCTCTCCGTTGACGGTCGACGGCATCAAGATC ACCAACGTCGAGGGCGAGGGTTGCGGGACGTGCAACATCGCCGAGGCGCTCAAAATGTCGCT GAACACCTCCTACTACCGGCTGATGCTCAAGCTCAACGGCGGCCCACAGGCTGTGGCCGATGC

CGCGCACCAAGCCGGCATTGCCTCCAGCTTCCCGGGCGTTGCGCACACGCTGTCCGAAGATGG CAAGGGTGGACCGCCCAACAACGGGATCGTGTTGGGCCAGTACCAAACCCGGGTGATCGACAT GGCATCGGCGTATGCCACGTTGGCCGCGTCCGGTATCTACCACCCGCCGCATTTCGTACAGAA GGTGGTCAGTGCCAACGGCCAGGTCCTCTTCGACGCCAGCACCGCGGACAACACCGGCGATCA GCGCATCCCCAAGGCGGTAGCCGACAACGTGACTGCGGCGATGGAGCCGATCGCAGGTTATTC

GCGTGGCCACAACCTAGCGGGTGGGCGGGATTCGGCGGCCAAGACCGGCACTACGCAATTTG GTGACACCACCGCGAACAAAGACGCCTGGATGGTCGGGTACACGCCGTCGTTGTCTACGGCTG TGTGGGTGGGCACCGTCAAGGGTGACGAGCCACTGGTAACCGCTTCGGGTGCAGCGATTTACG GCTCGGGCCTGCCGTCGGACATCTGGAAGGCAACCATGGACGGCGCCTTGAAGGGCACGTCG AACGAGACTTTCCCCAAACCGACCGAGGTCGGTGGTTATGCCGGTGTGCCGCCGCCGCCGCCG

CCGCCGGAGGTACCACCTTCGGAGACCGTCATCCAGCCCACGGTCGAAATTGCGCCGGGGATT ACCATCCCGATCGGTCCCCCGACCACCATTACCCTGGCGCCACCGCCCCCGGCCCCGCCCGCT GCGACTCCCACGCCGCCGCCGTGA

>Rv0051 - TB.seq 55694:57373 MW:61210

>emb|AL123456|MTBH37RV:55694-57376, Rv0051 SEQ ID NO: 13 GTGACCGGCGCGCTGTCCCAAAGCAGCAACATCTCGCCACTTCCTTTGGCCGCCGATCTGCGG AGCGCCGATAACCGCGATTGCCCCAGCCGCACCGACGTATTGGGTGCCGCTCTGGCGAATGTC GTCGGTGGCCCGGTAGGCCGGCACGCGCTGATCGGCCGCACCCGGCTGATGACCCCGCTGCG GGTGATGTTTGCAATCGCGTTGGTGTTCCTGGCGCTCGGTTGGTCGACGAAAGCGGCCTGCTT GCAGTCCACCGGAACCGGTCCAGGTGATCAGCGGGTGGCCAACTGGGATAACCAGCGTGCTTA

CTACCAGTTGTGCTACTCCGATACGGTGCCGCTCTATGGCGCTGAGTTATTGAGCCAAGGCAAG TTTCCGTACAAATCAAGCTGGATCGAAACCGACAGCAACGGCACACCGCAGCTGCGCTACGAC GGACAGATCGCGGTGCGCTATATGGAGTATCCGGTGCTGACTGGGATCTATCAGTACCTGTCGA TGGCGATAGCCAAGACCTACACCGCGTTAAGCAAGGTGGCTCCCCTCCCGGTGGTTGCCGAAG TGGTGATGTTCTTCAACGTCGCCGCGTTCGGTTTGGCGCTGGCGTGGCTGACAACCGTCTGGG

CGACCTCGGGCCTGGCCGGCCGCCGGATATGGGATGCGGCGCTGGTGGCCGCCTCACCGCTG GTGATCTTTCAGATATTCACCAATTTCGATGCGCTGGCAACGGGTTTGGCGACGAGTGGGCTGC TGGCCTGGGCGCGGCGCAGACCGGTGCTTGCCGGTGTGCTGATCGGGTTGGGCTCCGCGGCG AAACTGTATCCGCTGTTGTTCTTGTACCCGTTGTTGCTGCTGGGCATCCGGGCCGGTCGCCTGA ATGCTCTGGCCCGCACCATGGCGGCCGCGGCGGCGACCTGGTTGTTGGTGAATCTGCCGGTGA

TGCTGCTCTTTCCGCGCGGCTGGTCGGAGTTCTTCCGGCTCAACACCCGGCGCGGCGACGACA TGGACTCGTTGTACAACGTCGTCAAGTCGTTCACCGGCTGGCGTGGCTTCGACCCCACCCTGG GCTTCTGGGAGCCGCCGCTGGTGCTGAACACGGTTGTCACGCTCTTGTTCGTGTTATGTTGTGC GGCAATTGCTTACATCGCGCTCACCGCACCCCACCGGCCGCGCGTGGCGCAGCTGACTTTCTT GACGGTGGCCAGCTTCCTGTTGGTCAACAAGGTGTGGAGTCCCCAGTTCTCGCTTTGGCTGGTG

CCGCTGGCCGTGCTGGCTTTGCCGCACCGCCGGATCTTGCTGGCGTGGATGACGATCGACGCG TTGGTGTGGGTGCCGCGGATGTACTACCTATACGGCAACCCGAGCCGCTCGCTGCCCGAGCAG TGGTTCACCACGACGGTGTTGCTGCGTGACATCGCCGTGATGGTGCTGTGCGGACTGGTGGTC TGGCAGATCTACCGCCCCGGGCGCGACCTCGTGCGTACCGGCGGGCCAGGGGCACTGCCGGC TTGTGGGGGAGTCGACGACCCGGTGGGAGGGGTCTTTGCCAACGCCGCCGACGCCCCGCCAG

GTCGGCTACCGTCGTGGCTGCGTCCCCGGCTGGGCGACGAGCATGCGCGAGAGAGGACGCCC GATGCAGGTCGCGATCGCACTTTTTCCGGGCAACACCGCGCTTGA

>Rv0106 - TB.seq 124372:125565 MW:43701 >emb|AL123456|MTBH37RV:124372-125568, Rv0106 SEQ ID NO:14

ATGCGTACTCCGGTGATATTGGTGGCAGGTCAGGATCACACCGACGAGGTGACGGGCGCCTTG TTGCGCCGGACCGGAACGGTGGTCGTGGAGCACCGGTTTGACGGCCATGTGGTGCGACGGAT GACTGCCACGCTGAGCCGTGGCGAATTGATCACCACGGAGGACGCTTTGGAGTTCGCCCACGG CTGTGTGTCGTGCACAATCCGCGACGACCTGCTGGTGCTGTTACGCAGACTGCACCGCCGAGA CAATGTCGGCCGGATCGTCGTGCACCTGGCGCCGTGGCTGGAGCCCCAGCCCATCTGCTGGG

CGATCGACCACGTGCGGGTTTGCGTCGGACACGGATACCCAGACGGACCAGCCGCCCTCGAC GTGCGGGTCGCGGCCGTGGTGACCTGTGTGGACTGCGTAAGGTGGCTGCCGCAGTCACTCGG CGAGGACGAACTGCCCGACGGGCGCACGGTGGCCCAAGTGACGGTCGGTCAGGCCGAGTTCG CCGACCTTCTGGTGCTGACCCACCCGGAACCGGTCGCCGTGGCGGTTCTGCGCCGACTGGCC CCTCGAGCGCGAATCACCGGCGGCGTCGACCGCGTCGAGCTGGCGCTGGCGCATCTGGACGA CAACTCACGGAGGGGTCGTACCGATACCCCGCACACGCCATTGCTGGCGGGCCTGCCTCCGTT GGCAGCCGACGGTGAGGTTGCGATCGTGGAATTCAGTGCCCGCCGCCCGTTTCACCCGCAACG

TCTGCATGCCGCGGTTGACCTGCTGCTCGATGGCGTGGTTCGCACTCGAGGTCGGCTGTGGCT GGCCAACCGGCCGGATCAGGTCATGTGGCTCGAATCAGCCGGTGGCGGTCTGCGGGTCGCAT CGGCCGGAAAGTGGTTGGCGGCGATGGCGGCCTCGGAGGTGGCCTATGTCGACCTGGAGCGG CGGTTGTTCGCCGACCTGATGTGGGTCTACCCGTTCGGAGACCGGCACACCGCGATGACGGTA CTGGTATGCGGCGCCGATCCGACCGACATCGTCAATGCCCTGAACGCGGCGCTGCTCAGCGAC

GACGAAATGGCATCTCCGCAACGCTGGCAGTCCTACGTCGACCCTTTCGGCGACTGGCATGAC GACCCGTGCCACGAAATGCCCGATGCGGCTGGGGAATTCTCGGCACACCGCAACTCAGGAGAA TCTCGATGA

>Rv0125 - TB.seq 151146:152210 MW:34927

>emb|AL123456|MTBH37RV:151146-152213, pepA SEQ ID NO:15

ATGAGCAATTCGCGCCGCCGCTCACTCAGGTGGTCATGGTTGCTGAGCGTGCTGGCTGCCGTC GGGCTGGGCCTGGCCACGGCGCCGGCCCAGGCGGCCCCGCCGGCCTTGTCGCAGGACCGGT TCGCCGACTTCCCCGCGCTGCCCCTCGACCCGTCCGCGATGGTCGCCCAAGTGGGGCCACAG GTGGTCAACATCAACACCAAACTGGGCTACAACAACGCCGTGGGCGCCGGGACCGGCATCGTC

ATCGATCCCAACGGTGTCGTGCTGACCAACAACCACGTGATCGCGGGCGCCACCGACATCAAT GCGTTCAGCGTCGGCTCCGGCCAAACCTACGGCGTCGATGTGGTCGGGTATGACCGCACCCAG GATGTCGCGGTGCTGCAGCTGCGCGGTGCCGGTGGCCTGCCGTCGGCGGCGATCGGTGGCG GCGTCGCGGTTGGTGAGCCCGTCGTCGCGATGGGCAACAGCGGTGGGCAGGGCGGAACGCC CCGTGCGGTGCCTGGCAGGGTGGTCGCGCTCGGCCAAACCGTGCAGGCGTCGGATTCGCTGA

CCGGTGCCGAAGAGACATTGAACGGGTTGATCCAGTTCGATGCCGCGATCCAGCCCGGTGATT CGGGCGGGCCCGTCGTCAACGGCCTAGGACAGGTGGTCGGTATGAACACGGCCGCGTCCGAT AACTTCCAGCTGTCCCAGGGTGGGCAGGGATTCGCCATTCCGATCGGGCAGGCGATGGCGATC GCGGGCCAGATCCGATCGGGTGGGGGGTCACCCACCGTTCATATCGGGCCTACCGCCTTCCTC GGCTTGGGTGTTGTCGACAACAACGGCAACGGCGCACGAGTCCAACGCGTGGTCGGGAGCGC

TCCGGCGGCAAGTCTCGGCATCTCCACCGGCGACGTGATCACCGCGGTCGACGGCGCTCCGAT CAACTCGGCCACCGCGATGGCGGACGCGCTTAACGGGCATCATCCCGGTGACGTCATCTCGGT GACCTGGCAAACCAAGTCGGGCGGCACGCGTACAGGGAACGTGACATTGGCCGAGGGACCCC CGGCCTGA

>Rv0350 dnaK 70 kD heat shock protein, chromosome replication TB.seq 419833:421707 MW:66832 SEQ ID NO:16 >emb|AL123456|MTBH37RV:419833-421710, dnaK

ATGGCTCGTGCGGTCGGGATCGACCTCGGGACCACCAACTCCGTCGTCTCGGTTCTGGAAGGT GGCGACCCGGTCGTCGTCGCCAACTCCGAGGGCTCCAGGACCACCCCGTCAATTGTCGCGTTC GCCCGCAACGGTGAGGTGCTGGTCGGCCAGCCCGCCAAGAACCAGGCAGTGACCAACGTCGA TCGCACCGTGCGCTCGGTCAAGCGACACATGGGCAGCGACTGGTCCATAGAGATTGACGGCAA

GAAATACACCGCGCCGGAGATCAGCGCCCGCATTCTGATGAAGCTGAAGCGCGACGCCGAGGC CTACCTCGGTGAGGACATTACCGACGCGGTTATCACGACGCCCGCCTACTTCAATGACGCCCAG CGTCAGGCCACCAAGGACGCCGGCCAGATCGCCGGCCTCAACGTGCTGCGGATCGTCAACGA GCCGACCGCGGCCGCGCTGGCCTACGGCCTCGACAAGGGCGAGAAGGAGCAGCGAATCCTGG TCTTCGACTTGGGTGGTGGCACTTTCGACGTTTCCCTGCTGGAGATCGGCGAGGGTGTGGTTGA

GGTCCGTGCCACTTCGGGTGACAACCACCTCGGCGGCGACGACTGGGACCAGCGGGTCGTCG ATTGGCTGGTGGACAAGTTCAAGGGCACCAGCGGCATCGATCTGACCAAGGACAAGATGGCGA TGCAGCGGCTGCGGGAAGCCGCCGAGAAGGCAAAGATCGAGCTGAGTTCGAGTCAGTCCACCT CGATCAACCTGCCCTACATCACCGTCGACGCCGACAAGAACCCGTTGTTCTTAGACGAGCAGCT GACCCGCGCGGAGTTCCAACGGATCACTCAGGACCTGCTGGACCGCACTCGCAAGCCGTTCCA

GTCGGTGATCGCTGACACCGGCATTTCGGTGTCGGAGATCGATCACGTTGTGCTCGTGGGTGG TTCGACCCGGATGCCCGCGGTGACCGATCTGGTCAAGGAACTCACCGGCGGCAAGGAACCCAA CAAGGGCGTCAACCCCGATGAGGTTGTCGCGGTGGGAGCCGCTCTGCAGGCCGGCGTCCTCA AGGGCGAGGTGAAAGACGTTCTGCTGCTTGATGTTACCCCGCTGAGCCTGGGTATCGAGACCA AGGGCGGGGTGATGACCAGGCTCATCGAGCGCAACACCACGATCCCCACCAAGCGGTCGGAG

ACTTTCACCACCGCCGACGACAACCAACCGTCGGTGCAGATCCAGGTCTATCAGGGGGAGCGT GAGATCGCCGCGCACAACAAGTTGCTCGGGTCCTTCGAGCTGACCGGCATCCCGCCGGCGCC GCGGGGGATTCCGCAGATCGAGGTCACTTTCGACATCGACGCCAACGGCATTGTGCACGTCAC CGCCAAGGACAAGGGCACCGGCAAGGAGAACACGATCCGAATCCAGGAAGGCTCGGGCCTGT CCAAGGAAGACATTGACCGCATGATCAAGGACGCCGAAGCGCACGCCGAGGAGGATCGCAAGC

GTCGCGAGGAGGCCGATGTTCGTAATCAAGCCGAGACATTGGTCTACCAGACGGAGAAGTTCG TCAAAGAACAGCGTGAGGCCGAGGGTGGTTCGAAGGTACCTGAAGACACGCTGAACAAGGTTG ATGCCGCGGTGGCGGAAGCGAAGGCGGCACTTGGCGGATCGGATATTTCGGCCATCAAGTCG GCGATGGAGAAGCTGGGCCAGGAGTCGCAGGCTCTGGGGCAAGCGATCTACGAAGCAGCTCA GGCTGCGTCACAGGCCACTGGCGCTGCCCACCCCGGCGGCGAGCCGGGCGGTGCCCACCCC

GGCTCGGCTGATGACGTTGTGGACGCGGAGGTGGTCGACGACGGCCGGGAGGCCAAGTGA

>Rv0351 grpE stimulates DnaK ATPase activity TB.seq 421707:422411 MW:24501 >emb|AL123456|MTBH37RV:421707-422414, grpE SEQ ID N0:17 GTGACGGACGGAAATCAAAAGCCGGATGGCAATTCGGGCGAACAGGTAACCGTCACTGACAAG

CGGCGGATCGATCCCGAGACGGGTGAAGTGCGGCACGTCCCTCCCGGCGACATGCCGGGAGG GACGGCTGCGGCCGATGCGGCGCACACCGAAGACAAGGTCGCCGAGCTGACCGCCGATCTGC AACGCGTGCAGGCCGACTTCGCCAACTACCGTAAGCGGGCGTTGCGCGATCAGCAGGCGGCC GCTGACCGAGCCAAGGCCAGCGTTGTCAGCCAATTGCTGGGTGTACTGGACGATCTCGAGCGG GCGCGCAAGCACGGCGATTTGGAGTCGGGTCCACTGAAGTCGGTCGCCGACAAGCTAGACAGC GCGTTGACCGGGCTGGGTCTGGTGGCGTTCGGTGCCGAGGGCGAGGATTTCGACCCCGTGCT GCACGAAGCGGTGCAACACGAGGGCGACGGCGGGCAGGGGTCCAAGCCGGTAATCGGCACC

GTCATGCGGCAGGGCTACCAACTGGGTGAGCAGGTGCTGCGGCACGCCTTGGTCGGCGTCGT CGACACGGTGGTCGTCGACGCGGCCGAACTGGAGTCAGTCGACGACGGCACTGCGGTCGCAG ATACCGCCGAAAACGATCAAGCTGACCAGGGCAATAGCGCCGACACCTCGGGCGAACAGGCAG AATCAGAACCGTCGGGCAGTTAA

>Rv0352 dnaJ acts with GrpE to stimulate DnaK ATPase TB.seq 422450:423634 MW:41346 >emb|AL123456|MTBH37RV:422450-423637, dnaJ SEQ ID NO:18

ATGGCCCAAAGGGAATGGGTCGAAAAAGACTTCTACCAGGAGCTGGGCGTCTCCTCTGATGCC AGTCCTGAAGAGATCAAACGTGCCTATCGGAAGTTGGCGCGCGACCTGCATCCGGACGCGAAC CCGGGCAACCCGGCCGCCGGCGAACGGTTCAAGGCGGTTTCGGAGGCGCATAACGTGCTGTC

GGATCCGGCCAAGCGCAAGGAGTACGACGAAACCCGCCGCCTGTTCGCCGGCGGCGGGTTCG GCGGCCGTCGGTTCGACAGCGGCTTTGGGGGCGGGTTCGGCGGTTTCGGGGTCGGTGGAGAC GGCGCCGAGTTCAACCTCAACGACTTGTTCGACGCCGCCAGCCGAACCGGCGGTACCACCATC GGTGACTTGTTCGGTGGCTTGTTCGGACGCGGTGGCAGCGCCCGTCCCAGCCGCCCGCGACG CGGCAACGACCTGGAGACCGAGACCGAGTTGGATTTCGTGGAGGCCGCCAAGGGCGTGGCGA

TGCCGCTGCGATTAACCAGCCCGGCGCCGTGCACCAACTGCCATGGCAGCGGGGCCCGGCCA GGCACCAGCCCAAAGGTGTGTCCCACTTGCAACGGGTCGGGCGTGATCAACCGCAATCAGGGC GCGTTCGGCTTCTCCGAGCCGTGCACCGACTGCCGAGGTAGCGGCTCGATCATCGAGCACCCC TGCGAGGAGTGCAAAGGCACCGGCGTGACCACCCGCACCCGAACCATCAACGTGCGGATCCC GCCCGGTGTCGAGGATGGGCAGCGCATCCGGCTAGCCGGTCAGGGCGAGGCCGGGTTGCGC

GGCGCTCCCTCGGGGGATCTCTACGTGACGGTGCATGTGCGGCCCGACAAGATCTTCGGCCGC GACGGCGACGACCTCACCGTCACCGTTCCGGTCAGCTTCACCGAATTGGCTTTGGGCTCGACG CTGTCGGTGCCTACCCTGGACGGCACGGTCGGGGTCCGGGTGCCCAAAGGCACCGCTGACGG CCGCATTCTGCGTGTGCGCGGACGCGGTGTGCCCAAGCGCAGTGGGGGTAGCGGCGACCTAC TTGTCACCGTGAAGGTGGCCGTGCCGCCCAATTTGGCAGGCGCCGCTCAGGAAGCTCTGGAAG

CCTATGCGGCGGCGGAGCGGTCCAGTGGTTTCAACCCGCGGGCCGGATGGGCAGGTAATCGC TGA

>Rv0363c fba fructose bisphosphate aldolase TB.seq 441266:442297 MW:36545 >emb|AL123456|MTBH37RV:c442297-441263, fba SEQ ID NO:19

ATGCCTATCGCAACGCCCGAGGTCTACGCGGAGATGCTCGGTCAGGCCAAACAAAACTCGTAC GCTTTCCCGGCTATCAACTGCACCTCCTCGGAAACCGTCAACGCCGCGATCAAAGGTTTCGCCG ACGCCGGCAGTGACGGAATCATCCAGTTCTCGACCGGTGGCGCAGAATTCGGCTCCGGCCTCG GGGTCAAAGACATGGTGACCGGTGCGGTCGCCTTGGCGGAGTTCACCCACGTTATCGCGGCCA AGTACCCGGTCAACGTGGCGCTGCACACCGACCACTGCCCCAAGGACAAGTTGGACAGCTATG TCCGGCCCTTGCTGGCGATCTCGGCGCAACGCGTGAGCAAAGGTGGCAATCCTTTGTTCCAGT CGCACATGTGGGACGGCTCGGCAGTGCCAATCGATGAGAACCTGGCCATCGCCCAGGAGCTGC

TCAAGGCGGCGGCGGCCGCCAAGATCATTCTGGAGATCGAGATCGGCGTCGTCGGCGGCGAA GAGGACGGCGTGGCGAACGAGATCAACGAGAAGCTGTACACCAGCCCGGAGGACTTCGAGAAA ACCATCGAGGCGCTGGGCGCCGGTGAGCACGGCAAATACCTGCTGGCCGCGACGTTCGGCAA CGTGCATGGCGTCTACAAGCCCGGCAACGTCAAGCTTCGCCCCGACATCCTTGCGCAAGGGCA ACAGGTGGCGGCGGCCAAGCTCGGACTGCCGGCCGACGCCAAGCCGTTCGACTTCGTGTTCC

ACGGCGGCTCGGGTTCGCTTAAGTCGGAGATCGAGGAGGCGCTGCGCTACGGCGTGGTGAAG ATGAACGTCGACACCGACACCCAGTACGCGTTCACCCGCCCGATCGCCGGTCACATGTTCACC AACTACGACGGAGTGCTCAAGGTCGATGGCGAGGTGGGTGTCAAGAAGGTCTACGACCCGCGC AGCTACCTCAAGAAGGCCGAAGCTTCGATGAGCCAGCGGGTCGTTCAGGCGTGCAATGACCTG CACTGCGCCGGAAAGTCCCTAACCCACTAA

>Rv0405 pks6 TB.seq 485729:489934 MW:147615 >emb|AL123456|MTBH37RV:485729-489937, pks6 SEQ ID NO:20 ATGACAGACGGTTCGGTCACTGCGGATAAGCTTCAAAAATGGTTTCGAGAGTACTTGTCCACGC ATATCGAGTGTCATCCAAATGAGGTCAGCCTAGACGTTCCGATTAGAGATTTAGGTTTGAAATCG

ATTGATGTCTTAGCGATTCCCGGCGACCTCGGTGACAGATTTGGGTTTTGTATTCCCGATTTGGC CGTTTGGGATAATCCTAGCGCTAATGATTTGATTGATAGTCTGTTGAACCAGCGTAGTGCTGACT CGTTAAGAGAGAGTCATGGACACGCCGACAGGAACACGCAGGGTCGGGGCAGCATAAACGAGC CGGTTGCGGTCATCGGAGTGGGCTGTCGATTTCCGGGAGATATTGACGGCCCGGAACGGCTAT GGGACTTTCTGACCGAGAAGAAGTGTGCGATAACAGCGTATCCAGATCGTGGGTTCACGAATGC

TGGAACTTTCGCGGAGTCCGGAGGCTTTTTAAAGGATGTCGCGGGTTTCGATAATAGATTTTTTG ATATCCCGCCGGACGAGGCTCTGCGAATGGATCCGCAACAACGGTTGTTACTGGAGGTCTCTTG GGAAGCGTTAGAGCATGCAGGAATTATTCCTGAGTCATTAAGACTTTCACGTACGGGCGTATTC GTTGGGGTGTCGTCAACTGACTACGTCCGGCTTGTGTCAGCTAGCGCTCAGCAAAAGTCTACTA TTTGGGATAACACCGGCGGTTCTTCGAGTATTATTGCCAATAGAATCTCATACTTTCTCGATATTC

AGGGTCCGTCCATTGTCATTGACACGGCATGCTCGTCATCCCTGGTCGCCGTGCATCTAGCCTG TCGAAGTCTCAGTACCTGGGACTGCGATATCGCACTTGTCGGTGGGACGAATGTTCTTATTTCAC CAGAACCATGGGGTGGGTTTAGGGAAGCGGGCATCTTGTCGCAGACAGGCTGCTGTCACGCGT TCGATAAATCCGCCGACGGGATGGTACGCGGTGAGGGATGCGGAGTTATCGTGCTGCAGCGCC TCAGTGATGCACGCCTTGAGGGCCGGCGGATATTAGCGATTCTGACGGGTTCAGCGGTCAATC

AGGACGGTAAGTCCAACGGTATTATGGCGCCAAATCCTAGTGCGCAAATTGGTGTTCTTGAAAAT GCATGCAAGAGCGCTCGCGTCGATCCGCTGGAAATCGGCTACGTCGAGGCCCACGGGACCGG AACGTCGTTAGGGGATAGGATCGAGGCGCACGCCTTAGGCATGGTCTTTGGTCGCAAGAGACC GGGATCTGGGCCCCTGATGATCGGGAGCATCAAGCCGAATATCGGCCATCTGGAAGGTGCGGC TGGCATCGCCGGATTGATCAAGGCGGTGTTGATGGTTGAGCGTGGCTCGCTGCTTCCGAGCGG GGGGTTTACGGAGCCAAATCCAGCTATCCCATTCACGGAATTGGGCCTGAGAGTTGTAGACGAA CTTCAGGAGTGGCCGGTGGTGGCGGGTCGGCCGCGCCGGGCTGGGGTGTCATCGTTCGGCTT

TGGCGGCACCAATGCGCATGTGATTGTCGAGGAAGCTGGTTCGGTTGGGGCGGACACGGTTTC GGGCCGCGCGGATGTTGGCGGTTCCGGTGGTGGGGTGGTGGCGTGGGTGATTTCGGGGAAGA CGGCTTCGGCGTTGGCTGCTCAGGCGGGTCGGTTGGGGCGGTATGTGCGGGCTCGGCCGGCG CTTGATGTTGTTGATGTGGGGTATTCGTTGGTGAGCACGCGGTCGGTGTTTGATCATCGGGCGG TGGTGGTCGGCCAGACTCGCGATGAGTTGCTGGCTGGGTTGGCTGGGGTGGTTGCTGGTCGG

CCGGAGGCTGGGGTGGTCTGCGGTGTTGGCAAGCCGGCGGGCAAGACGGCTTTTGTGTTTGC CGGTCAGGGCTCGCAGTGGCTGGGTATGGGTAGCGAGCTTTATGCTGCCTACCCGGTTTTCGC CGAGGCCCTCGATGCTGTGGTGGACGAGTTGGACCGGCACCTGCGGTATCCGCTGCGCGATGT GATCTGGGGGCACGACCAAGATCTGTTGAATACCACCGAATTCGCCCAGCCGGCGCTGTTTGC GGTGGAGGTGGCGCTGTATCGGCTGCTCATGTCGTGGGGGGTGCGGCCGGGTTTGGTGCTGG

GTCATTCGGTGGGCGAGTTGGCCGCGGCGCACGTCGCCGGGGCGCTGTGTTTGCCGGATGCG GCGATGCTGGTGGCCGCGCGTGGACGGTTGATGCAGGCGTTGCCCGCCGGCGGCGCCATGTT TGCGGTGCAGGCCCGTGAAGACGAGGTAGCGCCGATGCTGGGGCACGATGTGAGCATCGCGG CGGTCAATGGTCCGGCTTCGGTGGTGATCTCTGGTGCCCACGATGCGGTGAGCGCGATCGCTG ATCGGCTGCGCGGCCAGGGCCGTCGGGTCCACCGGTTGGCGGTCTCGCATGCCTTTCACTCG

GCGTTGATGGAGCCGATGATCGCTGAGTTCACAGCCGTTGCGGCCGAACTGTCTGTGGGCTTG CCCACGATCCCGGTCATTTCCAATGTGACCGGGCAGTTGGTGGCCGACGACTTCGCCTCAGCT GATTACTGGGCCCGGCATATCCGGGCGGTGGTGCGGTTTGGCGACAGTGTTCGTAGTGCCCAC TGCGCCGGTGCCAGTCGTTTCATCGAAGTCGGGCCCGGTGGCGGCTTGACGTCGTTGATCGAG GCATCGCTGGCCGACGCGCAGATCGTGTCGGTGCCCACGCTGCGCAAAGATCGGCCCGAACC

GGTCAGTGTGATGACGGCGGCGGCCCAGGGCTTCGTCTCGGGGATGGGCCTGGATTGGGCCT CGGTGTTTTCCGGGTACCGGCCCAAGCGGGTGGAGTTGCCGACGTATGCCTTCCAGCATCAAA AGTTCTGGCTCGCACCAGCCCCATCGGTCAGCGACCCCACCGCCGCCGGCCAGATCGGGGCT AGCGATGGTGGTGCTGAACTCTTGGCGTCCTCCGGGTTTGCCGCCCGGCTGGCCGGTCGGTCG GCCGACGAGCAACTCGCCGCAGCGATCGAGGTGGTATGTGAGCATGCCGCAGCGGTGCTGGG

GCGCGACGGCGCTGCCGGACTCGACGCTGGCCAGGCGTTTGCCGATTCGGGATTTAATTCCTT GAGTGCCGTGGAGCTACGTAACCGCTTAACAGCCGTCACCGCAGTAACGCTGCCGGCCACCGC GATCTTCGATCACCCCACCCCGACCGAACTAGCCCAGTATCTGATCACCCAAATAGACGGTCAC GGCAGCTCCGCCGCCGCAGCGGCAAACCCGGCGGAGCGAATCGATGCGCTCACCGATCTTTTT CTACAAGCTTGCGATGCGGGTCGGGATGCCGATGGTTGGAAGATGGTCGCCCTGGCGTCGAAT

ACGCGCGAGCGCATGAGCTCACCGGTTCGGAACAACGTATCGAAGAACGTCGCACTGCTGGCA GATGGTATCTCCGATGTGGTTGTAATTTGTATCCCAACTCTAACTGTGCTATCGGATCAGCGTGA ATATCGAGATATTGCGAATGCGATGACAGGCCGCCATTCGGTTTATTCGCTTACGCTTCCCGGG TTCGATTCGTCTGATGCACTGCCGCAAAACGCGGATATGATTGTTGAAACCGTATCTAACGCAAT TATTGATGTGGTAGGCGGCAGCTGCCGTTTTGTGCTGTCGGGCTATTCATCGGGTGGGGTGTTG GCCTATGCCCTCTGCTCCCATCTGTCGGTCAAGCACCAGCGGAATCCCCTCGGAGTCGCACTCA TCGATACATATCTGCCTAGTCAGATCGCCAATCCTTCAATGAATGAAGGGTTCAGCCCCAACGAT

ACTGGGAAGGGCCTTTCCCGTGAAGTAATTCGAGTGGCCAGAATGTTGAATCGGTTAACTGCCA CCCGACTCACCGCGGCAGCCACCTATGCTGCAATCTTTCAGGCCTGGGAACCAGGTAGATCAAT GGCTCCGGTTCTTAACATCGTGGCGAAGGACCGAATAGCTACCGTCGAAAATTTACGCGAAGAA CGAATCAACCGGTGGCGAACTGCTGCTGCAGAGGCGGCCTATTCTGTAGCCGAAGTACCCGGG GATCATTTCGGAATGATGAGCACCTCGAGTGAGGCAATAGCTACCGAAATACATGATTGGATTTC

TGGGCTCGTTCGAGGGCCTCATCGGTAG

>Rv0435c - ATPase of AAA-family TB.seq 522348:524531 MW:75315 >emb|AL123456|MTBH37RV:c524531 -522345, Rv0435c SEQ ID N0:21 GTGACCCACCCGGACCCGGCCCGCCAACTCACCCTTACCGCCCGGCTGAACACCTCGGCCGTC

GACTCACGCCGCGGCGTCGTTCGGTTGCACCCCAATGCCATTGCTGCCCTTGGCATCCGCGAG TGGGACGCGGTGTCGCTGACCGGCTCTCGGACAACCGCCGCGGTCGCCGGCCTGGCCGCGGC AGACACCGCGGTCGGGACGGTGCTGCTCGATGACGTCACACTGTCCAATGCGGGCCTTCGCGA AGGCACCGAGGTGATCGTCAGCCCGGTCACCGTCTACGGAGCGCGATCGGTGACGCTGAGCG GTTCAACGCTGGCCACCCAGTCGGTGCCGCCGGTCACGCTGCGGCAGGCCCTACTCGGCAAG

GTGATGACCGTCGGTGACGCGGTCTCGCTGCTGCCCCGCGATCTAGGCCCCGGCACATCCACG TCGGCTGCCAGCCGCGCATTGGCAGCTGCGGTCGGGATCAGTTGGACCTCGGAGCTGCTGACC GTTACCGGCGTCGACCCCGACGGGCCGGTCAGCGTGCAGCCCAACTCGCTGGTCACCTGGGG CGCTGGGGTCCCGGCCGCAATGGGTACGTCCACGGCCGGGCAAGTGAGCATCTCGAGTCCGG AGATCCAGATCGAAGAGCTCAAGGGCGCCCAGCCGCAGGCTGCCAAGCTCACCGAATGGCTCA

AGCTTGCCCTCGATGAGCCGCACCTACTACAGACCTTGGGCGCCGGCACCAATTTGGGTGTGC TGGTGTCGGGTCCGGCCGGGGTGGGCAAGGCGACGCTGGTGCGCGCGGTGTGCGACGGCCG AAGGTTGGTGACACTGGATGGTCCGGAGATTGGAGCTCTGGCCGCCGGAGACCGGGTCAAAGC CGTGGCCTCGGCAGTGCAGGCGGTTCGCCATGAGGGCGGTGTGTTGCTGATCACCGATGCCGA CGCCCTGCTGCCAGCCGCCGCCGAGCCGGTAGCCTCGCTGATCCTGTCCGAGCTGCGTACCG

CGGTGGCCACCGCCGGTGTGGTATTGATCGCCACCTCAGCACGGCCCGATCAACTCGATGCCC GGCTGCGTTCCCCCGAGTTGTGCGACCGGGAGCTTGGCCTGCCGCTGCCCGACGCGGCCACC CGCAAATCGCTGCTGGAGGCGCTGCTGAATCCGGTTCCTACCGGAGACCTCAACCTCGACGAA ATCGCCTCCCGCACACCGGGTTTCGTCGTGGCCGACCTGGCTGCGCTGGTTCGCGAGGCGGC GCTGCGGGCAGCGTCTCGAGCCAGTGCCGACGGCCGACCACCGATGCTGCACCAAGACGACC

TCCTCGGTGCGTTGACCGTCATCCGGCCGCTGTCCCGCTCGGCCAGCGACGAAGTCACCGTGG GTGACGTGACGCTCGACGATGTCGGTGACATGGCCGCGGCCAAACAAGCACTGACCGAGGCG GTGCTGTGGCCGCTGCAGCACCCCGACACCTTCGCTCGGCTAGGTGTCGAACCGCCGCGCGG GGTGTTGCTGTACGGCCCGCCCGGCTGCGGCAAGACCTTTGTGGTTCGTGCCCTGGCCAGCAC CGGACAGTTGAGCGTGCATGCCGTCAAAGGGTCGGAGCTGATGGACAAGTGGGTGGGCTCCTC GGAGAAGGCAGTCCGCGAGCTATTCCGGCGGGCCCGCGACTCCGCGCCGTCACTGGTGTTCC TCGACGAGCTGGACGCTCTGGCGCCACGGCGCGGTCAGAGCTTCGACTCGGGCGTCTCCGAC

CGGGTGGTGGCCGCGCTGCTGACTGAGCTCGACGGTATTGACCCGCTGCGGGATGTCGTCATG CTAGGCGCGACCAACCGGCCCGATCTGATAGACCCGGCGCTGCTGCGCCCGGGGCGGCTAGA ACGGCTGGTGTTCGTTGAACCGCCCGACGCTGCCGCTCGCCGCGAAATCCTGCGCACCGCTGG CAAGTCGATCCCGCTGAGCTCCGACGTCGACCTGGACGAGGTGGCAGCCGGACTCGACGGTTA TAGTGCCGCCGACTGTGTGGCGCTGCTGCGCGAAGCCGCGCTTACCGCGATGCGGCGTTCCAT

CGATGCCGCCAACGTCACCGCCGCCGACCTGGCGACCGCGCGAGAAACCGTGCGCGCGTCGC TGGATCCGCTGCAGGTGGCGTCGCTGCGTAAGTTCGGCACCAAGGGTGACCTTCGGTCCTAG

>Rv0436c pssA CDP-diacylglycerol-serine o-phosphatidyltransferase TB.seq 524531 :525388 MW:31219 >emb|AL123456|MTBH37RV:c525388-524528, pssA SEQ ID NO:22

ATGATCGGAAAGCCCCGCGGCAGGCGAGGGGTAAACCTGCAGATACTGCCCAGCGCGATGAC GGTGCTGTCCATTTGCGCGGGACTGACCGCAATCAAGTTTGCGCTCGAGCACCAGCCGAAGGC CGCGATGGCACTGATCGCCGCAGCGGCCATCCTCGACGGGCTCGACGGCCGGGTGGCCCGCA TCCTGGATGCCCAGTCGCGGATGGGCGCAGAGATCGACTCACTGGCCGACGCGGTGAACTTCG GAGTGACACCCGCGCTGGTGCTTTACGTGTCGATGTTGTCGAAGTGGCCGGTCGGTTGGGTGG

TCGTGCTGCTCTACGCGGTGTGCGTGGTATTACGGCTGGCGCGGTACAACGCACTGCAGGACG ACGGAACCCAGCCCGCCTACGCGCATGAATTCTTCGTCGGAATGCCCGCGCCGGCGGGCGCG GTTTCCATGATCGGCCTGCTAGCCCTCAAAATGCAGTTCGGCGAAGGATGGTGGACCTCGGGCT GGTTCCTCAGCTTTTGGGTGACGGGAACGTCGATACTCTTGGTCAGCGGGATCCCGATGAAAAA GATGCACGCCGTGTCGGTACCACCCAACTACGCGGCCGCCCTGCTGGCGGTGCTGGCTATCTG

CGCGGCGGCCGCAGTCCTGGCCCCCTACTTGTTGATCTGGGTGATCATCATCGCCTACATGTGC CATATTCCTTTCGCGGTGCGCAGCCAGCGCTGGCTTGCCCAACACCCTGAGGTGTGGGACGAC AAGCCCAAGCAACGGCGCGCGGTGCGGCGCGCGAGCCGCCGGGCGCATCCCTACCGGCCGT CGATGGCGCGGCTGGGCCTGCGCAAGCCGGGTCGACGGCTGTGA

>Rv0440 groEL 260 kD chaperonin 2 TB.seq 528606:530225 MW:56728 >emb|AL123456|MTBH37RV:528606-530228, groEL2 SEQ ID NO:23

ATGGCCAAGACAATTGCGTACGACGAAGAGGCCCGTCGCGGCCTCGAGCGGGGCTTGAACGC CCTCGCCGATGCGGTAAAGGTGACATTGGGCCCCAAGGGCCGCAACGTCGTCCTGGAAAAGAA GTGGGGTGCCCCCACGATCACCAACGATGGTGTGTCCATCGCCAAGGAGATCGAGCTGGAGGA

TCCGTACGAGAAGATCGGCGCCGAGCTGGTCAAAGAGGTAGCCAAGAAGACCGATGACGTCGC CGGTGACGGCACCACGACGGCCACCGTGCTGGCCCAGGCGTTGGTTCGCGAGGGCCTGCGCA ACGTCGCGGCCGGCGCCAACCCGCTCGGTCTCAAACGCGGCATCGAAAAGGCCGTGGAGAAG GTCACCGAGACCCTGCTCAAGGGCGCCAAGGAGGTCGAGACCAAGGAGCAGATTGCGGCCAC CGCAGCGATTTCGGCGGGTGACCAGTCCATCGGTGACCTGATCGCCGAGGCGATGGACAAGGT GGGCAACGAGGGCGTCATCACCGTCGAGGAGTCCAACACCTTTGGGCTGCAGCTCGAGCTCAC CGAGGGTATGCGGTTCGACAAGGGCTACATCTCGGGGTACTTCGTGACCGACCCGGAGCGTCA

GGAGGCGGTCCTGGAGGACCCCTACATCCTGCTGGTCAGCTCCAAGGTGTCCACTGTCAAGGA TCTGCTGCCGCTGCTCGAGAAGGTCATCGGAGCCGGTAAGCCGCTGCTGATCATCGCCGAGGA CGTCGAGGGCGAGGCGCTGTCCACCCTGGTCGTCAACAAGATCCGCGGCACCTTCAAGTCGGT GGCGGTCAAGGCTCCCGGCTTCGGCGACCGCCGCAAGGCGATGCTGCAGGATATGGCCATTCT CACCGGTGGTCAGGTGATCAGCGAAGAGGTCGGCCTGACGCTGGAGAACGCCGACCTGTCGC

TGCTAGGCAAGGCCCGCAAGGTCGTGGTCACCAAGGACGAGACCACCATCGTCGAGGGCGCC GGTGACACCGACGCCATCGCCGGACGAGTGGCCCAGATCCGCCAGGAGATCGAGAACAGCGA CTCCGACTACGACCGTGAGAAGCTGCAGGAGCGGCTGGCCAAGCTGGCCGGTGGTGTCGCGG TGATCAAGGCCGGTGCCGCCACCGAGGTCGAACTCAAGGAGCGCAAGCACCGCATCGAGGAT GCGGTTCGCAATGCCAAGGCCGCCGTCGAGGAGGGCATCGTCGCCGGTGGGGGTGTGACGCT

GTTGCAAGCGGCCCCGACCCTGGACGAGCTGAAGCTCGAAGGCGACGAGGCGACCGGCGCCA ACATCGTGAAGGTGGCGCTGGAGGCCCCGCTGAAGCAGATCGCCTTCAACTCCGGGCTGGAGC CGGGCGTGGTGGCCGAGAAGGTGCGCAACCTGCCGGCTGGCCACGGACTGAACGCTCAGACC GGTGTCTACGAGGATCTGCTCGCTGCCGGCGTTGCTGACCCGGTCAAGGTGACCCGTTCGGCG CTGCAGAATGCGGCGTCCATCGCGGGGCTGTTCCTGACCACCGAGGCCGTCGTTGCCGACAAG

CCGGAAAAGGAGAAGGCTTCCGTTCCCGGTGGCGGCGACATGGGTGGCATGGATTTCTGA

>Rv0482 murB TB.seq 570537:571643 MW:38522 >emb|AL123456|MTBH37RV:570537-571646, murB SEQ ID NO:24 ATGAAACGGAGCGGTGTCGGTTCGCTCTTTGCCGGTGCGCATATTGCCGAGGCGGTCCCGTTG

GCGCCGCTGACCACTTTGCGTGTGGGCCCGATCGCCCGACGTGTCATCACTTGCACCAGCGCC GAACAGGTGGTGGCTGCGCTGCGGCACCTGGATTCGGCGGCCAAGACCGGAGCTGACCGCCC GCTGGTGTTTGCTGGTGGCTCCAATTTGGTGATCGCCGAGAACCTGACCGACCTGACCGTGGT GCGGTTGGCCAATAGCGGCATCACCATCGACGGTAACTTGGTGCGGGCCGAGGCCGGTGCGG TCTTCGATGACGTGGTGGTTAGGGCCATCGAACAGGGTCTGGGCGGACTGGAATGCCTGTCTG

GCATCCCAGGATCGGCCGGGGCGACACCCGTGCAGAACGTGGGGGCGTATGGCGCGGAGGT GTCTGACACCATCACTCGGGTTCGGCTTTTGGATCGGTGCACGGGTGAGGTGCGTTGGGTATC CGCGCGCGACCTGCGCTTCGGCTATCGCACGAGCGTGCTCAAACACGCTGATGGGCTTGCGGT GCCCACCGTGGTCTTGGAGGTGGAGTTTGCGCTGGATCCGTCGGGCCGCAGCGCACCGCTGC GCTACGGCGAGCTGATCGCCGCGCTGAATGCGACCAGCGGCGAGCGCGCCGACCCGCAAGCG

GTCCGCGAAGCGGTGCTGGCCCTGCGGGCACGCAAGGGCATGGTGCTGGACCCGACCGACCA TGACACCTGGAGCGTGGGATCGTTCTTCACAAACCCGGTGGTCACCCAGGATGTTTACGAACGG CTGGCCGGTGACGCGGCCACCAGAAAGGACGGTCCGGTCCCGCACTATCCCGCGCCCGACGG CGTCAAGCTGGCCGCCGGCTGGCTGGTGGAACGGGCCGGCTTCGGCAAGGGCTATCCGGATG CCGGCGCCGCCCCATGCCGGCTTTCCACCAAACATGCGCTGGCGCTGACAAATCGTGGCGGG GCCACCGCCGAAGATGTGGTGACGCTGGCGCGCGCCGTGCGCGATGGGGTCCATGATGTGTTT GGTATCACACTAAAACCCGAACCCGTGCTGATCGGCTGCATGTTGTAG

>Rv0483 - TB.seq 571708:573060 MW:47859

>emb|AL123456|MTBH37RV:571708-573063, Rv0483 SEQ ID NO:25 GTGGTCATTCGTGTGCTGTTTCGCCCGGTATCTTTGATACCCGTGAATAACTCCAGCACCCCCCA GAGTCAGGGGCCGATCAGTCGGCGTCTGGCGTTGACGGCCCTTGGGTTTGGGGTGTTGGCACC

GAACGTTCTGGTCGCGTGCGCCGGCAAAGTGACCAAGCTGGCCGAGAAGAGGCCGCCACCGG CGCCTCGTCTGACTTTCCGGCCTGCCGACTCTGCCGCCGACGTGGTGCCGATCGCGCCGATCA GCGTCGAGGTCGGTGACGGCTGGTTTCAGCGGGTCGCGCTGACCAATTCGGCAGGCAAGGTC GTCGCCGGGGCATACAGCCGGGATCGCACCATCTACACGATCACCGAGCCGCTGGGCTACGAC ACGACCTACACCTGGAGCGGTTCGGCCGTCGGCCATGACGGCAAGGCGGTTCCGGTGGCGGG

CAAGTTCACCACCGTGGCACCCGTCAAGACGATCAACGCGGGATTCCAGCTCGCCGACGGCCA GACCGTCGGGATCGCGGCGCCGGTGATTATTCAGTTCGATTCACCGATCAGCGACAAGGCCGC CGTCGAGCGGGCACTAACCGTGACCACCGACCCGCCTGTCGAGGGCGGCTGGGCCTGGCTGC CCGACGAGGCGCAGGGCGCTCGCGTGCACTGGCGTCCTCGGGAGTACTACCCGGCGGGTACC ACCGTCGACGTCGACGCCAAGCTGTATGGGCTGCCGTTCGGCGACGGCGCGTACGGCGCGCA

GGATATGTCGTTGCACTTCCAGATCGGTCGTCGTCAGGTGGTCAAGGCCGAAGTCTCGTCGCAC CGCATCCAAGTCGTCACCGATGCCGGCGTCATCATGGACTTCCCGTGCAGCTACGGCGAGGCC GACTTGGCGCGCAACGTCACCCGCAACGGCATCCACGTCGTCACCGAGAAATACTCGGACTTC TACATGTCCAACCCGGCCGCCGGTTACAGCCATATCCACGAACGTTGGGCGGTGCGGATTTCC AACAACGGCGAGTTCATCCATGCCAACCCTATGAGCGCCGGTGCCCAGGGCAACAGCAATGTC

ACCAACGGCTGTATCAACCTGTCGACGGAGAACGCCGAACAGTACTACCGCAGCGCGGTCTAC GGTGACCCGGTTGAGGTGACCGGCAGTTCGATCCAGCTGTCCTACGCCGACGGTGACATCTGG GACTGGGCGGTGGACTGGGACACCTGGGTGTCGATGTCGGCGCTACCGCCACCGGCGGCCAA ACCGGCGGCGACGCAAATCCCGGTCACCGCCCCGGTCACGCCGTCGGATGCCCCCACCCCGT CCGGCACACCCACGACTACTAACGGACCGGGTGGGTAG

>Rv0489 gpm phosphoglycerate mutase I TB.seq 578424:579170 MW:27217 >emb|AL123456|MTBH37RV:578424-579173, gpm SEQ ID NO:26

ATGGCAAACACTGGCAGCCTGGTGTTGCTGCGCCACGGCGAGAGCGACTGGAATGCCCTCAAC CTGTTCACCGGCTGGGTCGATGTCGGCCTGACGGACAAGGGCCAGGCAGAGGCGGTTCGAAG

CGGCGAGCTGATCGCGGAACACGACCTATTGCCCGACGTGCTCTACACCTCGTTGCTGCGGCG CGCGATCACCACCGCGCATCTGGCGTTGGACAGCGCCGATCGGCTCTGGATTCCCGTGCGGCG TAGCTGGCGGCTCAACGAACGCCACTACGGCGCGCTGCAGGGTTTGGACAAGGCCGAGACCAA GGCCCGCTATGGCGAAGAGCAGTTCATGGCCTGGCGGCGCAGCTATGACACGCCGCCGCCGC CGATCGAGCGGGGCAGTCAGTTCAGCCAGGACGCCGACCCTCGTTACGCCGACATCGGCGGT GGCCCGCTCACCGAATGTCTGGCTGACGTGGTCGCCCGGTTTTTGCCATATTTCACCGACGTCA TCGTTGGCGACTTGCGGGTCGGCAAGACGGTGCTGATCGTTGCCCACGGCAACTCGTTGCGCG

CGCTGGTCAAGCACCTGGACCAGATGTCTGACGACGAAATCGTCGGACTGAACATCCCGACCG GAATTCCGCTGCGCTACGACCTGGATTCCGCGATGAGGCCGCTGGTGCGCGGTGGTACGTATC TGGACCCGGAGGCGGCAGCCGCCGGCGCCGCCGCGGTGGCCGGCCAGGGCCGCGGGTAA

>Rv0490 senX 3sensor histidine kinase TB.seq 579347:580576 MW:44794

>emb|AL123456|MTBH37RV:579347-580579, senX3 SEQ ID NO:27

GTGACTGTGTTCTCGGCGCTGTTGCTGGCCGGGGTTTTGTCCGCGCTGGCACTGGCCGTCGGT GGTGCTGTTGGAATGCGGCTGACGTCGCGGGTCGTCGAACAGCGCCAACGGGTGGCCACGGA GTGGTCGGGAATCACGGTTTCGCAGATGTTGCAATGCATTGTCACGCTGATGCCGCTGGGCGC CGCGGTGGTGGACACCCATCGCGACGTTGTCTACCTCAACGAACGGGCCAAAGAGCTAGGTCT

GGTGCGCGACCGCCAGCTCGATGATCAGGCCTGGCGGGCCGCCCGGCAGGCGCTGGGTGGT GAAGACGTCGAGTTCGACCTGTCGCCGCGCAAGCGGTCGGCCACGGGTCGATCCGGGCTATC AGTGCATGGGCATGCCCGGTTGCTGAGCGAGGAAGACCGCCGGTTCGCCGTGGTGTTCGTGCA CGACCAGTCGGATTATGCGCGGATGGAGGCGGCTAGGCGTGACTTCGTGGCCAACGTCAGTCA CGAGCTCAAGACGCCCGTCGGTGCCATGGCTCTACTCGCCGAGGCGCTGCTGGCGTCGGCCG

ACGACTCCGAAACCGTTCGGCGGTTCGCCGAGAAGGTGCTCATTGAGGCCAACCGGCTCGGTG ACATGGTCGCCGAGTTGATCGAGCTATCCCGGCTACAGGGCGCCGAGCGGCTACCCAATATGA CCGACGTCGACGTCGATACGATTGTGTCGGAAGCGATTTCACGCCATAAGGTGGCGGCCGACA ACGCCGACATCGAAGTCCGCACCGACGCGCCCAGCAATCTGCGGGTGCTGGGCGACCAAACTC TGCTGGTTACCGCACTGGCAAACCTGGTTTCCAATGCGATTGCCTATTCGCCGCGCGGGTCGCT

GGTGTCGATCAGCCGTCGCCGTCGCGGTGCCAACATCGAGATCGCCGTCACCGACCGGGGCA TCGGCATCGCGCCGGAAGACCAGGAGCGGGTCTTCGAACGGTTCTTCCGGGGGGACAAGGCG CGCTCGCGTGCCACCGGAGGCAGCGGACTCGGGTTGGCCATCGTCAAACACGTCGCGGCTAAT CACGACGGCACCATCCGCGTGTGGAGCAAACCGGGAACCGGGTCAACGTTCACCTTGGCTCTT CCGGCGTTGATCGAGGCCTATCACGACGACGAGCGACCCGAGCAGGCGCGAGAGCCCGAACT

GCGGTCAAACAGGTCACAACGAGAGGAAGAGCTGAGCCGATGA

>Rv0500 proC pyrroline-5-carboxylate reductase TB.seq 590081 :590965 MW:30172 >emb|AL123456|MTBH37RV:590081 -590968, proC SEQ ID NO:28 ATGCTTTTCGGCATGGCAAGGATCGCGATTATCGGCGGCGGCAGCATCGGTGAGGCATTGCTG

TCGGGTCTGCTGCGGGCGGGCCGGCAGGTCAAAGACCTGGTAGTGGCCGAGCGGATGCCCGA TCGCGCCAACTACCTGGCGCAGACCTATTCGGTGTTGGTGACGTCGGCGGCCGACGCGGTGGA GAACGCGACGTTCGTCGTCGTCGCGGTCAAACCAGCCGACGTCGAGCCGGTGATCGCGGATCT GGCGAACGCGACTGCGGCGGCCGAAAACGACAGTGCTGAGCAGGTGTTCGTCACCGTGGTAG CGGGCATCACGATCGCGTATTTCGAATCCAAGCTACCGGCTGGGACGCCAGTGGTGCGTGCGA TGCCGAACGCGGCGGCATTGGTGGGAGCGGGGGTTACAGCGCTGGCCAAAGGCCGCTTTGTC ACCCCGCAACAGCTTGAGGAGGTCTCGGCCTTGTTCGACGCGGTCGGCGGCGTGCTGACCGTT

CCGGAATCGCAGTTGGACGCGGTGACCGCGGTGTCCGGCTCGGGTCCGGCCTATTTCTTTCTG CTGGTCGAGGCCCTGGTGGATGCCGGAGTCGGGGTGGGCTTGAGCCGTCAGGTGGCCACCGA TCTCGCCGCGCAGACAATGGCTGGCTCAGCGGCGATGCTGCTGGAGCGGATGGAGCAAGACC AGGGTGGCGCCAATGGCGAGCTGATGGGGCTGCGCGTGGACCTTACCGCATCACGGCTGCGC GCCGCGGTTACCTCGCCGGGCGGTACG ACCGCCGCTGCGCTGCGGGAACTCGAACGCGGCG

GGTTTCGGATGGCTGTCGACGCGGCGGTTCAAGCCGCCAAAAGCCGCTCTGAGCAGCTCAGAA TTACACCGGAATGA

>Rv0528 - TB.seq 618303:619889 MW:57132 >emb|AL123456|MTBH37RV:618303-619892, Rv0528 SEQ ID NO:29 ATGTGGCGGTCGTTGACGTCGATGGGCACCGCGCTGGTGCTGCTGTTTTTGCTCGCGCTGGCT

GCCATACCCGGGGCCCTGCTGCCGCAGCGTGGCCTCAACGCCGCCAAGGTGGACGACTACCT GGCCGCGCACCCACTCATCGGTCCGTGGCTGGACGAGCTGCAGGCCTTCGACGTGTTCTCCAG CTTCTGGTTCACCGCCATCTACGTGCTGCTGTTCGTGTCCCTCGTCGGCTGTCTGGCCCCGCGG ACGATCGAGCACGCCCGCAGCCTGCGGGCTACACCGGTCGCCGCCCCGCGCAACCTGGCCCG GCTGCCCAAGCACGCCCACGCCCGGCTGGCCGGCGAGCCCGCCGCCCTGGCCGCCACCATCA

CGGGCCGGCTGCGCGGCTGGCGCAGCATCACCCGGCAACAAGGCGACAGCGTGGAAGTCTCC GCCGAGAAGGGCTACCTGCGCGAGTTCGGCAACCTGGTGTTCCACTTCGCGCTGCTGGGTCTG CTGGTGGCGGTGGCCGTCGGCAAGCTGTTCGGCTACGAGGGCAACGTGATCGTGATAGCCGA CGGCGGACCCGGTTTTTGTTCGGCGTCGCCGGCCGCGTTCGACTCGTTTCGCGCCGGCAACAC CGTCGACGGCACGTCGTTGCACCCGATCTGTGTGCGGGTCAACAACTTCCAAGCGCACTACCT

GCCGTCCGGGCAGGCCACCTCGTTCGCCGCCGACATCGACTATCAGGCCGACCCGGCCACTG CTGACCTGATCGCCAACAGCTGGCGGCCCTACCGGCTGCAGGTCAATCACCCGCTGCGGGTCG GCGGCGACCGGGTGTACCTGCAGGGCCACGGCTATGCGCCCACCTTCACCGTGACGTTCCCG GACGGGCAGACCCGCACGTCGACCGTGCAGTGGCGACCCGACAACCCGCAGACCCTGCTGTC GGCGGGCGTCGTGCGCATCGACCCGCCGGCCGGCAGCTACCCCAACCCCGACGAGCGTCGCA

AACACCAGATCGCCATCCAGGGCCTGCTGGCTCCCACCGAGCAGCTCGACGGCACCCTGCTGT CGTCGCGTTTCCCCGCGCTCAATGCCCCGGCGGTGGCCATCGACATCTACCGCGGCGACACCG GCCTGGACAGCGGGCGGCCCCAGTCGTTGTTCACCCTGGACCACCGGCTGATCGAGCAGGGC CGGCTGGTCAAGGAAAAGCGGGTCAACCTGCGCGCCGGTCAGCAAGTCCGCATCGACCAAGG CCCGGCGGCCGGCACGGTGGTCCGGTTCGACGGCGCGGTGCCGTTCGTCAACCTGCAGGTCT

CCCACGACCCCGGCCAGTCCTGGGTGCTGGTCTTCGCAATCACGATGATGGCGGGACTGCTGG TGTCGCTGCTGGTGCGCAGGCGCCGGGTGTGGGCGCGGATCACGCCGACGACCGCGGGTACG GTAAACGTCGAGCTGGGCGGCCTGACGCGCACCGACAACTCCGGGTGGGGCGCCGAGTTCGA

GCGGCTGACCGGGCGGTTGCTGGCGGGTTTTGAGGCGCGGTCCCCGGACATGGCCGAAGCGG

CCGCAGGGACCGGAAGGGACGTCGATTGA

>Rv0667 rpoB [beta] subunit of RNA polymerase TB.seq 759805:763320 MW:129220

>emb|AL123456|MTBH37RV:759805-763323, rpoB SEQ ID NO:30

TTGGCAGATTCCCGCCAGAGCAAAACAGCCGCTAGTCCTAGTCCGAGTCGCCCGCAAAGTTCCT CGAATAACTCCGTACCCGGAGCGCCAAACCGGGTCTCCTTCGCTAAGCTGCGCGAACCACTTG AGGTTCCGGGACTCCTTGACGTCCAGACCGATTCGTTCGAGTGGCTGATCGGTTCGCCGCGCT GGCGCGAATCCGCCGCCGAGCGGGGTGATGTCAACCCAGTGGGTGGCCTGGAAGAGGTGCTC

TACGAGCTGTCTCCGATCGAGGACTTCTCCGGGTCGATGTCGTTGTCGTTCTCTGACCCTCGTT TCGACGATGTCAAGGCACCCGTCGACGAGTGCAAAGACAAGGACATGACGTACGCGGCTCCAC TGTTCGTCACCGCCGAGTTCATCAACAACAACACCGGTGAGATCAAGAGTCAGACGGTGTTCAT GGGTGACTTCCCGATGATGACCGAGAAGGGCACGTTCATCATCAACGGGACCGAGCGTGTGGT GGTCAGCCAGCTGGTGCGGTCGCCCGGGGTGTACTTCGACGAGACCATTGACAAGTCCACCGA

CAAGACGCTGCACAGCGTCAAGGTGATCCCGAGCCGCGGCGCGTGGCTCGAGTTTGACGTCGA CAAGCGCGACACCGTCGGCGTGCGCATCGACCGCAAACGCCGGCAACCGGTCACCGTGCTGC TCAAGGCGCTGGGCTGGACCAGCGAGCAGATTGTCGAGCGGTTCGGGTTCTCCGAGATCATGC GATCGACGCTGGAGAAGGACAACACCGTCGGCACCGACGAGGCGCTGTTGGACATCTACCGCA AGCTGCGTCCGGGCGAGCCCCCGACCAAAGAGTCAGCGCAGACGCTGTTGGAAAACTTGTTCT

TCAAGGAGAAGCGCTACGACCTGGCCCGCGTCGGTCGCTATAAGGTCAACAAGAAGCTCGGGC TGCATGTCGGCGAGCCCATCACGTCGTCGACGCTGACCGAAGAAGACGTCGTGGCCACCATCG AATATCTGGTCCGCTTGCACGAGGGTCAGACCACGATGACCGTTCCGGGCGGCGTCGAGGTGC CGGTGGAAACCGACGACATCGACCACTTCGGCAACCGCCGCCTGCGTACGGTCGGCGAGCTG ATCCAAAACCAGATCCGGGTCGGCATGTCGCGGATGGAGCGGGTGGTCCGGGAGCGGATGAC

CACCCAGGACGTGGAGGCGATCACACCGCAGACGTTGATCAACATCCGGCCGGTGGTCGCCG CGATCAAGGAGTTCTTCGGCACCAGCCAGCTGAGCCAATTCATGGACCAGAACAACCCGCTGTC GGGGTTGACCCACAAGCGCCGACTGTCGGCGCTGGGGCCCGGCGGTCTGTCACGTGAGCGTG CCGGGCTGGAGGTCCGCGACGTGCACCCGTCGCACTACGGCCGGATGTGCCCGATCGAAACC CCTGAGGGGCCCAACATCGGTCTGATCGGCTCGCTGTCGGTGTACGCGCGGGTCAACCCGTTC

GGGTTCATCGAAACGCCGTACCGCAAGGTGGTCGACGGCGTGGTTAGCGACGAGATCGTGTAC CTGACCGCCGACGAGGAGGACCGCCACGTGGTGGCACAGGCCAATTCGCCGATCGATGCGGA CGGTCGCTTCGTCGAGCCGCGCGTGCTGGTCCGCCGCAAGGCGGGCGAGGTGGAGTACGTGC CCTCGTCTGAGGTGGACTACATGGACGTCTCGCCCCGCCAGATGGTGTCGGTGGCCACCGCGA TGATTCCCTTCCTGGAGCACGACGACGCCAACCGTGCCCTCATGGGGGCAAACATGCAGCGCC

AGGCGGTGCCGCTGGTCCGTAGCGAGGCCCCGCTGGTGGGCACCGGGATGGAGCTGCGCGC GGCGATCGACGCCGGCGACGTCGTCGTCGCCGAAGAAAGCGGCGTCATCGAGGAGGTGTCGG CCGACTACATCACTGTGATGCACGACAACGGCACCCGGCGTACCTACCGGATGCGCAAGTTTG CCCGGTCCAACCACGGCACTTGCGCCAACCAGTGCCCCATCGTGGACGCGGGCGACCGAGTC GAGGCCGGTCAGGTGATCGCCGACGGTCCCTGTACTGACGACGGCGAGATGGCGCTGGGCAA GAACCTGCTGGTGGCCATCATGCCGTGGGAGGGCCACAACTACGAGGACGCGATCATCCTGTC CAACCGCCTGGTCGAAGAGGACGTGCTCACCTCGATCCACATCGAGGAGCATGAGATCGATGC

TCGCGACACCAAGCTGGGTGCGGAGGAGATCACCCGCGACATCCCGAACATCTCCGACGAGGT GCTCGCCGACCTGGATGAGCGGGGCATCGTGCGCATCGGTGCCGAGGTTCGCGACGGGGACA TCCTGGTCGGCAAGGTCACCCCGAAGGGTGAGACCGAGCTGACGCCGGAGGAGCGGCTGCTG CGTGCCATCTTCGGTGAGAAGGCCCGCGAGGTGCGCGACACTTCGCTGAAGGTGCCGCACGG CGAATCCGGCAAGGTGATCGGCATTCGGGTGTTTTCCCGCGAGGACGAGGACGAGTTGCCGGC

CGGTGTCAACGAGCTGGTGCGTGTGTATGTGGCTCAGAAACGCAAGATCTCCGACGGTGACAA GCTGGCCGGCCGGCACGGCAACAAGGGCGTGATCGGCAAGATCCTGCCGGTTGAGGACATGC CGTTCCTTGCCGACGGCACCCCGGTGGACATTATTTTGAACACCCACGGCGTGCCGCGACGGA TGAACATCGGCCAGATTTTGGAGACCCACCTGGGTTGGTGTGCCCACAGCGGCTGGAAGGTCG ACGCCGCCAAGGGGGTTCCGGACTGGGCCGCCAGGCTGCCCGACGAACTGCTCGAGGCGCAG

CCGAACGCCATTGTGTCGACGCCGGTGTTCGACGGCGCCCAGGAGGCCGAGCTGCAGGGCCT GTTGTCGTGCACGCTGCCCAACCGCGACGGTGACGTGCTGGTCGACGCCGACGGCAAGGCCA TGCTCTTCGACGGGCGCAGCGGCGAGCCGTTCCCGTACCCGGTCACGGTTGGCTACATGTACA TCATGAAGCTGCACCACCTGGTGGACGACAAGATCCACGCCCGCTCCACCGGGCCGTACTCGA TGATCACCCAGCAGCCGCTGGGCGGTAAGGCGCAGTTCGGTGGCCAGCGGTTCGGGGAGATG

GAGTGCTGGGCCATGCAGGCCTACGGTGCTGCCTACACCCTGCAGGAGCTGTTGACCATCAAG TCCGATGACACCGTCGGCCGCGTCAAGGTGTACGAGGCGATCGTCAAGGGTGAGAACATCCCG GAGCCGGGCATCCCCGAGTCGTTCAAGGTGCTGCTCAAAGAACTGCAGTCGCTGTGCCTCAAC GTCGAGGTGCTATCGAGTGACGGTGCGGCGATCGAACTGCGCGAAGGTGAGGACGAGGACCT GGAGCGGGCCGCGGCCAACCTGGGAATCAATCTGTCCCGCAACGAATCCGCAAGTGTCGAGGA

TCTTGCGTAA

>Rv0668 rpoC [beta]' subunit of RNA polymerase TB.seq 763368:767315 MW:146740 >emb|AL123456|MTBH37RV:763368-767318, rpoC SEQ ID NO:31 GTGCTCGACGTCAACTTCTTCGATGAACTCCGCATCGGTCTTGCTACCGCGGAGGACATCAGGC

AATGGTCCTATGGCGAGGTCAAAAAGCCGGAGACGATCAACTACCGCACGCTTAAGCCGGAGA AGGACGGCCTGTTCTGCGAGAAGATCTTCGGGCCGACTCGCGACTGGGAATGCTACTGCGGCA AGTACAAGCGGGTGCGCTTCAAGGGCATCATCTGCGAGCGCTGCGGCGTCGAGGTGACCCGC GCCAAGGTGCGTCGTGAGCGGATGGGCCACATCGAGCTTGCCGCGCCCGTCACCCACATCTG GTACTTCAAGGGTGTGCCCTCGCGGCTGGGGTATCTGCTGGACCTGGCCCCGAAGGACCTGGA

GAAGATCATCTACTTCGCTGCCTACGTGATCACCTCGGTCGACGAGGAGATGCGCCACAATGAG CTCTCCACGCTCGAGGCCGAAATGGCGGTGGAGCGCAAGGCCGTCGAAGACCAGCGCGACGG CGAACTAGAGGCCCGGGCGCAAAAGCTGGAGGCCGACCTGGCCGAGCTGGAGGCCGAGGGC GCCAAGGCCGATGCGCGGCGCAAGGTTCGCGACGGCGGCGAGCGCGAGATGCGCCAGATCC GTGACCGCGCGCAGCGTGAGCTGGACCGGTTGGAGGACATCTGGAGCACTTTCACCAAGCTGG CGCCCAAGCAGCTGATCGTCGACGAAAACCTCTACCGCGAACTCGTCGACCGCTACGGCGAGT ACTTCACCGGTGCCATGGGCGCGGAGTCGATCCAGAAGCTGATCGAGAACTTCGACATCGACG

CCGAAGCCGAGTCGCTGCGGGATGTCATCCGAAACGGCAAGGGGCAGAAGAAGCTTCGCGCC CTCAAGCGGCTGAAGGTGGTTGCGGCGTTCCAACAGTCGGGCAACTCGCCGATGGGCATGGTG CTCGACGCCGTCCCGGTGATCCCGCCGGAGCTGCGCCCGATGGTGCAGCTCGACGGCGGCCG GTTCGCCACGTCCGACTTGAACGACCTGTACCGCAGGGTGATCAACCGCAACAACCGGCTGAA AAGGCTGATCGATCTGGGTGCGCCGGAAATCATCGTCAACAACGAGAAGCGGATGCTGCAGGA

ATCCGTGGACGCGCTGTTCGACAATGGCCGCCGCGGCCGGCCCGTCACCGGGCCGGGCAACC GTCCGCTCAAGTCGCTTTCCGATCTGCTCAAGGGCAAGCAGGGCCGGTTCCGGCAGAACCTGC TCGGCAAGCGTGTCGACTACTCGGGCCGGTCGGTCATCGTGGTCGGCCCGCAGCTCAAGCTGC ACCAGTGCGGTCTGCCCAAGCTGATGGCGCTGGAGCTGTTCAAGCCGTTCGTGATGAAGCGGC TGGTGGACCTCAACCATGCGCAGAACATCAAGAGCGCCAAGCGCATGGTGGAGCGCCAGCGCC

CCCAAGTGTGGGATGTGCTCGAAGAGGTCATCGCCGAGCACCCGGTGTTGCTGAACCGCGCAC CCACCCTGCACCGGTTGGGTATCCAGGCCTTCGAGCCAATGCTGGTGGAAGGCAAGGCCATTC AGCTGCACCCGTTGGTGTGTGAGGCGTTCAATGCCGACTTCGACGGTGACCAGATGGCCGTGC ACCTGCCTTTGAGCGCCGAAGCGCAGGCCGAGGCTCGCATTTTGATGTTGTCCTCCAACAACAT CCTGTCGCCGGCATCTGGGCGTCCGTTGGCCATGCCGCGGCTGGACATGGTGACCGGGCTGT

ACTACCTGACCACCGAGGTCCCCGGGGACACCGGCGAATACCAGCCGGCCAGCGGGGATCAC CCGGAGACTGGTGTCTACTCTTCGCCGGCCGAAGCGATCATGGCGGCCGACCGCGGTGTCTTG AGCGTGCGGGCCAAGATCAAGGTGCGGCTGACCCAGCTGCGGCCGCCGGTCGAGATCGAGGC CGAGCTATTCGGCCACAGCGGCTGGCAGCCGGGCGATGCGTGGATGGCCGAGACCACGCTGG GCCGGGTGATGTTCAACGAGCTGCTGCCGCTGGGTTATCCGTTCGTCAACAAGCAGATGCACAA

GAAGGTGCAGGCCGCCATCATCAACGACCTGGCCGAGCGTTACCCGATGATCGTGGTCGCCCA GACCGTCGACAAGCTCAAGGACGCCGGCTTCTACTGGGCCACCCGCAGCGGCGTGACGGTGT CGATGGCCGACGTGCTGGTGCCGCCGCGCAAGAAGGAGATCCTCGACCACTACGAGGAGCGC GCGGACAAGGTCGAAAAGCAGTTCCAGCGTGGCGCTTTGAACCACGACGAGCGCAACGAGGC GCTGGTGGAGATTTGGAAGGAAGCCACCGACGAGGTCGGTCAGGCGTTGCGGGAGCACTACC

CCGACGACAACCCGATCATCACCATCGTCGACTCCGGCGCCACCGGCAACTTCACCCAGACTC GAACGCTGGCCGGTATGAAGGGCCTGGTGACCAACCCGAAGGGTGAGTTCATCCCGCGTCCG GTCAAGTCCTCCTTCCGTGAGGGCCTGACCGTGCTGGAGTACTTCATCAACACCCACGGCGCTC GAAAGGGCTTGGCGGACACCGCGTTGCGCACCGCCGACTCCGGCTACCTGACCCGACGTCTG GTGGACGTGTCCCAGGACGTGATCGTGCGCGAGCACGACTGCCAGACCGAGCGCGGCATCGT

CGTCGAGCTGGCCGAGCGTGCACCCGACGGCACGCTGATCCGCGACCCGTACATCGAAACCTC GGCCTACGCGCGGACCCTGGGCACCGACGCGGTCGACGAGGCCGGCAACGTCATCGTCGAGC GTGGTCAAGACCTGGGCGATCCGGAGATTGACGCTCTGTTGGCTGCTGGTATTACCCAGGTCAA GGTGCGTTCGGTGCTGACGTGTGCCACCAGCACCGGCGTGTGCGCGACCTGCTACGGGCGTT CCATGGCCACCGGCAAGCTGGTCGACATCGGTGAAGCCGTCGGCATCGTGGCCGCCCAGTCC ATCGGCGAACCCGGCACCCAGCTGACCATGCGCACCTTCCACCAGGGTGGCGTCGGTGAGGA CATCACCGGTGGTCTGCCCCGGGTGCAGGAGCTGTTCGAGGCCCGGGTACCGCGTGGCAAGG

CGCCGATCGCCGACGTCACCGGCCGGGTTCGGCTCGAGGACGGCGAGCGGTTCTACAAGATC ACCATCGTTCCTGACGACGGCGGTGAGGAAGTGGTCTACGACAAGATCTCCAAGCGGCAGCGG CTGCGGGTGTTCAAGCACGAAGACGGTTCCGAACGGGTGCTCTCCGATGGCGACCACGTCGAG GTGGGCCAGCAGCTGATGGAAGGCTCGGCCGACCCGCATGAGGTGCTGCGGGTGCAGGGCCC CCGCGAGGTGCAGATACACCTGGTTCGCGAGGTCCAGGAGGTCTACCGCGCCCAAGGTGTGTC

GATCCACGACAAGCACATCGAGGTGATCGTTCGCCAGATGCTGCGCCGGGTGACCATCATCGA CTCGGGCTCGACGGAGTTTTTGCCTGGCTCGCTGATCGACCGCGCGGAGTTCGAGGCAGAGAA CCGCCGAGTGGTGGCCGAGGGCGGTGAGCCCGCGGCCGGCCGTCCGGTGCTGATGGGCATC ACGAAGGCGTCGCTGGCCACCGACTCGTGGCTGTCGGCGGCGTCGTTCCAGGAGACCACTCG CGTGCTGACCGATGCGGCGATCAACTGCCGCAGCGATAAGCTCAACGGTCTGAAGGAAAACGT

GATCATCGGCAAGCTGATCCCGGCCGGTACCGGTATCAACCGCTACCGCAACATCGCGGTGCA GCCCACCGAGGAGGCCCGCGCTGCGGCGTACACCATCCCGTCGTATGAGGATCAGTACTACAG CCCGGACTTCGGTGCGGCCACCGGTGCTGCCGTCCCGCTGGACGACTACGGCTACAGCGACTA CCGCTAG

>Rv0711 atsA TB.seq 806333:808693 MW:86216 >emb|AL123456|MTBH37RV:806333-808696, atsA SEQ ID NO:32

ATGGCACCCGAGGCCACCGAGGCGTTCAACGGCACCATCGAGCTGGATATTCGTGATTCGGAG CCGGATTGGGGCCCATACGCAGCGCCGGTGGCACCGGAGCACTCACCAAACATCCTGTATCTG GTCTGGGACGACGTCGGCATCGCGACCTGGGACTGCTTTGGCGGCCTGGTCGAGATGCCCGC

GATGACGCGCGTCGCCGAGCGTGGCGTGCGACTGTCGCAATTTCACACCACCGCACTGTGCTC GCCGACCCGGGCGTCGCTGCTGACCGGTCGCAACGCCACCACCGTAGGCATGGCTACCATCG AAGAGTTCACCGACGGGTTCCCCAACTGCAACGGGCGGATCCCGGCTGACACCGCGTTGCTCC CAGAGGTGCTGGCCGAACATGGCTACAACACCTACTGTGTGGGCAAGTGGCACCTGACGCCAC TCGAAGAATCCAATATGGCGTCGACGAAGCGGCACTGGCCGACCTCGCGTGGGTTCGAGCGGT

TCTACGGATTCCTAGGCGGGGAGACCGACCAGTGGTATCCCGACCTGGTATACGACAACCACC CAGTGAGTCCTCCCGGCACACCCGAGGGTGGCTACCACCTGTCAAAAGACATCGCCGACAAGA CGATCGAGTTCATTCGTGATGCCAAGGTGATCGCGCCCGACAAGCCGTGGTTCAGCTACGTGTG CCCAGGCGCCGGGCATGCGCCGCACCACGTCTTCAAGGAATGGGCGGACAGATACGCCGGCC GATTCGACATGGGGTATGAGCGCTATCGCGAGATCGTGCTGGAAAGGCAAAAGGCGCTAGGGA

TCGTGCCACCCGACACCGAACTGTCGCCCATAAACCCTTATCTGGATGTGCCGGGGCCAAACG GCGAGACCTGGCCGCTGCAGGACACGGTGCGGCCGTGGGACTCGCTGAGCGATGAAGAAAAG AAGCTGTTTTGCCGGATGGCCGAGGTGTTCGCCGGCTTTCTGAGCTACACCGACGCCCAGATC GGACGGATCCTGGACTACCTCGAGGAATCCGGCCAGCTGGACAACACCATCATCGTGGTGATC TCCGACAACGGCGCCAGCGGCGAGGGCGGACCCAACGGATCGGTCAACGAAGGCAAGTTCTT CAACGGCTACATCGACACCGTCGCTGAAAGCATGAAGCTCTTCGACCACCTCGGTGGCCCGCA GACCTACAACCACTACCCCATCGGGTGGGCAATGGCCTTCAACACCCCCTACAAGCTGTTCAAG

CGCTACGCCTCGCATGAAGGCGGCATTGCCGACCCGGCAATCATCTCCTGGCCCAACGGCATT GCCGCACACGGTGAAATCCGCGACAACTACGTCAATGTCAGCGACATCACGCCCACCGTCTAC GACCTGTTGGGCATGACACCGCCGGGGACCGTCAAGGGGATTCCGCAGAAACCGATGGACGG CGTGAGCTTCATAGCGGCCCTTGCCGACCCGGCCGCCGACACCGGCAAGACCACCCAGTTCTA CACCATGCTGGGCACCCGCGGGATCTGGCATGAAGGTTGGTTCGCCAACACCATTCACGCGGC

CACGCCCGCCGGCTGGTCGAATTTCAACGCTGACCGCTGGGAACTGTTCCACATCGCAGCAGA CCGCAGCCAGTGCCACGACCTGGCCGCCGAGCATCCCGACAAACTTGAGGAGCTCAAGGCGCT GTGGTTCTCCGAAGCCGCCAAGTACAACGGGCTGCCGCTGGCCGATCTGAACCTCCTGGAAAC GATGACTCGGTCGCGGCCTTACCTGGTCAGCGAACGAGCCAGCTACGTCTACTATCCCGACTG CGCTGACGTCGGCATCGGCGCGGCCGTAGAGATTCGCGGGCGCTCGTTCGCCGTGCTGGCCG

ATGTGACCATCGATACCACCGGCGCCGAGGGCGTGCTGTTCAAGCACGGCGGCGCCCATGGC GGGCACGTGCTGTTCGTCCGGGACGGACGCTTGCACTACGTCTACAACTTCCTCGGTGAGCGC CAGCAGCTGGTCAGCTCGTCGGGTCCGGTCCCGTCGGGAAGACATCTACTCGGGGTTCGTTAT TTGCGGACCGGAACCGTGCCCAACAGTCACACGCCGGTGGGCGATCTTGAGCTGTTCTTCGAC GAGAACCTGGTCGGCGCCCTGACCAATGTGCTGACCCACCCTGGAACGTTCGGGTTGGCCGGC

GCCGCTATCAGCGTTGGCCGCAACGGCGGTTCGGCTGTGTCCAGCCACTACGAAGCGCCGTTC GCGTTCACCGGCGGTACCATCACCCAGGTCACCGTCGACGTGTCAGGCCGACCGTTCGAAGAT GTGGAATCCGATCTTGCGCTTGCTTTTTCGCGTGACTGA

>Rv0764c - lanosterol 14-demethylase cytochrome P450 TB.seq 856683:858035 MW:50879

>emb|AL123456|MTBH37RV:c858035-856680, Rv0764c SEQ ID NO:33

ATGAGCGCTGTTGCACTACCCCGGGTTTCGGGTGGCCACGACGAACACGGCCACCTCGAGGAG TTCCGCACCGATCCGATCGGGCTGATGCAACGGGTCCGCGACGAATGCGGAGACGTCGGTACC TTCCAGCTGGCCGGGAAGCAGGTCGTGCTGCTGTCCGGCTCGCACGCCAACGAATTCTTCTTC CGGGCGGGCGACGACGACCTGGACCAGGCCAAGGCATACCCGTTCATGACGCCGATCTTCGG

CGAGGGCGTGGTGTTCGACGCCAGCCCGGAACGGCGTAAAGAGATGCTGCACAATGCCGCGC TACGCGGCGAGCAGATGAAGGGCCACGCTGCCACCATCGAAGATCAAGTCCGACGGATGATCG CCGACTGGGGTGAGGCCGGCGAGATCGATCTGCTGGACTTCTTCGCCGAGCTGACCATCTACA CCTCCTCGGCCTGCCTGATCGGCAAGAAGTTCCGCGACCAGCTCGACGGGCGATTCGCCAAGC TCTATCACGAGTTGGAGCGCGGCACCGACCCACTAGCCTACGTCGACCCGTATCTGCCGATCG

AGAGCTTCCGTCGCCGCGACGAAGCCCGCAATGGTCTGGTGGCACTGGTTGCGGACATCATGA ACGGCCGGATCGCCAACCCACCCACCGACAAGAGCGACCGTGACATGCTCGACGTGCTCATCG CCGTCAAGGCTGAGACCGGCACTCCCCGGTTCTCGGCCGACGAGATCACCGGCATGTTCATCT CGATGATGTTCGCCGGCCATCACACCAGCTCGGGTACGGCTTCGTGGACGCTGATCGAGTTGA TGCGCCATCGCGACGCCTACGCGGCCGTGATCGACGAACTCGACGAGCTGTACGGCGACGGC CGATCGGTGAGTTTCCATGCGCTGCGCCAGATTCCGCAGCTGGAAAACGTGCTGAAAGAGACG CTGCGCCTGCACCCTCCGCTGATCATCCTCATGCGAGTGGCCAAGGGCGAGTTCGAGGTGCAA

GGCCACCGGATTCATGAGGGCGATCTGGTGGCGGCCTCCCCGGCGATCTCCAACCGGATCCCC GAAGACTTCCCCGATCCCCACGACTTCGTGCCAGCACGATACGAGCAGCCGCGCCAGGAAGAT CTGCTCAACCGCTGGACGTGGATTCCGTTCGGCGCCGGCCGGCATCGTTGCGTGGGGGCGGC GTTCGCCATCATGCAGATCAAAGCGATCTTCTCGGTGTTGTTGCGCGAGTATGAGTTTGAGATG GCGCAACCGCCAGAAAGCTATCGTAACGACCATTCGAAGATGGTGGTGCAGTTGGCCCAGCCC

GCTTGCGTGCGCTACCGCCGGCGAACGGGAGTTTAA

>Rv0861c - DNA helicase TB.seq 958524:960149 MW:59773 >emb|AL123456|MTBH37RV:c960149-958521 , Rv0861c SEQ ID NO:34 GTGCAGTCCGATAAGACGGTGCTGTTGGAAGTCGACCATGAACTGGCCGGCGCTGCACGCGCC

GCCATCGCGCCGTTCGCCGAGCTGGAACGTGCACCCGAACATGTCCACACCTACCGCATCACA CCGCTGGCACTGTGGAATGCTCGCGCCGCCGGCCATGATGCCGAGCAAGTCGTCGACGCGCT GGTCAGTTACTCCCGCTACGCGGTGCCGCAACCCTTGCTCGTCGACATCGTCGACACCATGGC CCGCTACGGACGACTGCAGTTGGTCAAGAACCCGGCCCATGGCCTGACGCTGGTGAGCCTGGA CCGCGCGGTGCTTGAGGAAGTGCTGCGCAACAAGAAGATCGCGCCGATGCTTGGCGCCCGCAT

CGATGACGACACCGTCGTCGTCCACCCCAGCGAACGCGGCCGGGTCAAGCAGCTGCTGCTCAA GATCGGTTGGCCCGCAGAGGATCTCGCCGGCTACGTCGATGGTGAAGCGCACCCGATCAGCCT GCACCAGGAGGGCTGGCAGCTGCGCGATTACCAGCGGCTGGCCGCGGACTCGTTCTGGGCGG GCGGCTCCGGGGTGGTGGTGCTGCCATGTGGGGCCGGCAAGACGCTGGTCGGTGCGGCCGC AATGGCCAAAGCCGGCGCGACGACGTTGATCCTGGTCACCAATATCGTCGCGGCCCGGCAATG

GAAACGAGAGCTGGTCGCGCGCACCTCGCTCACCGAGAATGAGATCGGCGAATTCTCGGGAGA ACGCAAGGAAATCCGACCTGTCACCATCTCGACATACCAGATGATCACCCGCCGCACTAAGGGC GAGTACCGCCATCTGGAACTGTTCGACAGCCGCGACTGGGGGCTCATCATCTATGACGAGGTG CACCTGTTGCCGGCACCGGTCTTCCGGATGACCGCTGACCTGCAGTCCAAACGGCGGCTGGGG CTGACCGCCACGTTGATCCGTGAAGACGGACGCGAGGGCGACGTGTTTTCCCTTATCGGACCA

AAGCGCTATGACGCGCCGTGGAAGGACATTGAGGCGCAGGGCTGGATCGCGCCAGCTGAGTG CGTGGAAGTCCGGGTCACGATGACCGACAGCGAGCGGATGATGTACGCCACCGCCGAACCCG AAGAACGCTACCGGATCTGCTCGACGGTGCACACCAAAATTGCTGTGGTCAAGTCGATTCTGGC GAAGCACCCGGATGAGCAGACCCTGGTCATCGGAGCGTACTTGGATCAGCTCGACGAGCTGGG CGCCGAGCTCGGCGCTCCGGTGATTCAGGGGTCGACAAGGACCAGCGAACGCGAGGCACTGT

TCGACGCCTTCCGCCGCGGCGAGGTCGCTACGCTCGTGGTGTCCAAGGTGGCTAACTTCTCCA TCGACTTGCCGGAAGCCGCCGTGGCGGTACAGGTTTCGGGAACATTCGGCTCACGCCAGGAAG AGGCGCAACGGCTCGGCCGGATATTGCGACCCAAGGCCGACGGGGGCGGTGCCATCTTCTAC

TCGGTGGTGGCCCGCGACAGCCTGGATGCCGAGTACGCCGCACACCGGCAGCGGTTTTTAGCT

GAGCAGGGCTACGGTTACATCATCCGCGACGCCGACGACCTGCTGGGCCCGGCAATTTAG

>Rv0904c accD3 TB.seq 1006694:1008178 MW:51741

>emb|AL123456|MTBH37RV:c1008178-1006691 , accD3 SEQ ID NO:35

GTGAGTCGTATCACGACCGACCAACTGCGGCACGCGGTGCTAGACCGGGGATCTTTCGTCAGC TGGGATAGCGAGCCGCTGGCGGTGCCGGTAGCCGACTCCTATGCGCGGGAGCTGGCCGCCGC TCGGGCGGCCACCGGCGCGGACGAATCGGTGCAGACCGGTGAGGGACGCGTATTCGGGCGG CGGGTGGCCGTGGTGGCCTGTGAGTTCGACTTCCTGGGCGGCTCGATTGGGGTGGCAGCGGC

CGAACGGATCACCGCCGCCGTCGAGCGGGCGACCGCCGAGCGGCTGCCGCTACTGGCGTCAC CAAGCTCGGGAGGCACCCGCATGCAAGAAGGCACGGTCGCGTTTCTGCAGATGGTGAAGATCG CTGCGGCCATCCAGCTGCACAACCAGGCGCGCCTGCCCTACCTGGTCTATTTGCGCCATCCGA CCACGGGTGGAGTTTTCGCGTCGTGGGGCTCGCTGGGGCATCTCACCGTCGCCGAGCCGGGC GCCCTGATCGGCTTTCTGGGACCACGGGTCTATGAGTTGCTCTATGGCGACCCCTTCCCATCCG

GCGTCCAAACCGCCGAGAATCTACGGCGGCATGGGATCATCGACGGCGTCGTTGCACTGGACC GGCTACGACCGATGCTGGATCGTGCGTTGACGGTGCTCATCGACGCTCCCGAACCGCTTCCGG CACCGCAGACGCCCGCGCCCGTACCCGATGTGCCCACGTGGGACTCGGTGGTGGCATCGCGC CGGCCGGACCGGCCGGGCGTCAGGCAGCTACTGCGACACGGCGCCACCGACCGGGTGTTGTT GTCAGGAACCGATCAAGGCGAAGCGGCGACCACGCTGCTGGCGCTGGCCCGCTTTGGCGGCC

AACCCACGGTGGTCCTCGGCCAGCAAAGGGCAGTAGGCGGCGGGGGAAGCACTGTCGGGCCC GCTGCGTTACGCGAAGCCCGACGCGGGATGGCGCTCGCCGCCGAGCTGTGCCTGCCGCTGGT GCTGGTCATTGACGCGGCCGGACCCGCGTTGTCGGCCGCAGCCGAACAGGGCGGGCTGGCCG GCCAGATCGCGCATTGCCTGGCCGAGCTCGTCACGCTGGATACCCCGACCGTGTCGATCCTGC TGGGCCAGGGCAGCGGCGGGCCGGCGCTGGCGATGTTGCCCGCCGACCGGGTGCTGGCCGC

ACTCCACGGCTGGCTGGCGCCCTTGCCTCCCGAAGGAGCCAGCGCGATCGTGTTCCGAGACAC TGCTCATGCCGCCGAACTCGCTGCCGCCCAAGGCATCCGGTCGGCCGACCTACTGAAGTCGGG GATTGTCGACACCATCGTGCCGGAGTACCCCGACGCCGCAGACGAGCCGATCGAGTTCGCCCT ACGACTGTCGAACGCCATCGCCGCCGAAGTGCACGCGTTACGGAAGATACCGGCCCCGGAACG CCTCGCGACTCGGTTGCAACGCTACCGCCGGATCGGGTTGCCCCGCGACTAA

>Rv0983 - TB.seq 1099064:1100455 MW:46454

>emb|AL123456|MTBH37RV:1099064-1100458, Rv0983 SEQ ID NO:36

ATGGCCAAGTTGGCCCGAGTAGTGGGCCTAGTACAGGAAGAGCAACCTAGCGACATGACGAAT CACCCACGGTATTCGCCACCGCCGCAGCAGCCGGGAACCCCAGGTTATGCTCAGGGGCAGCA

GCAAACGTACAGCCAGCAGTTCGACTGGCGTTACCCACCGTCCCCGCCCCCGCAGCCAACCCA GTACCGTCAACCCTACGAGGCGTTGGGTGGTACCCGGCCGGGTCTGATACCTGGCGTGATTCC GACCATGACGCCCCCTCCTGGGATGGTTCGCCAACGCCCTCGTGCAGGCATGTTGGCCATCGG CGCGGTGACGATAGCGGTGGTGTCCGCCGGCATCGGCGGCGCGGCCGCATCCCTGGTCGGGT TCAACCGGGCACCCGCCGGCCCCAGCGGCGGCCCAGTGGCTGCCAGCGCGGCGCCAAGCAT CCCCGCAGCAAACATGCCGCCGGGGTCGGTCGAACAGGTGGCGGCCAAGGTGGTGCCCAGTG TCGTCATGTTGGAAACCGATCTGGGCCGCCAGTCGGAGGAGGGCTCCGGCATCATTCTGTCTG

CCGAGGGGCTGATCTTGACCAACAACCACGTGATCGCGGCGGCCGCCAAGCCTCCCCTGGGC AGTCCGCCGCCGAAAACGACGGTAACCTTCTCTGACGGGCGGACCGCACCCTTCACGGTGGTG GGGGCTGACCCCACCAGTGATATCGCCGTCGTCCGTGTTCAGGGCGTCTCCGGGCTCACCCCG ATCTCCCTGGGTTCCTCCTCGGACCTGAGGGTCGGTCAGCCGGTGCTGGCGATCGGGTCGCCG CTCGGTTTGGAGGGCACCGTGACCACGGGGATCGTCAGCGCTCTCAACCGTCCAGTGTCGACG

ACCGGCGAGGCCGGCAACCAGAACACCGTGCTGGACGCCATTCAGACCGACGCCGCGATCAA CCCCGGTAACTCCGGGGGCGCGCTGGTGAACATGAACGCTCAACTCGTCGGAGTCAACTCGGC CATTGCCACGCTGGGCGCGGACTCAGCCGATGCGCAGAGCGGCTCGATCGGTCTCGGTTTTGC GATTCCAGTCGACCAGGCCAAGCGCATCGCCGACGAGTTGATCAGCACCGGCAAGGCGTCACA TGCCTCCCTGGGTGTGCAGGTGACCAATGACAAAGACACCCTGGGCGCCAAGATCGTCGAAGT

AGTGGCCGGTGGTGCTGCCGCGAACGCTGGAGTGCCGAAGGGCGTCGTTGTCACCAAGGTCG ACGACCGCCCGATCAACAGCGCGGACGCGTTGGTTGCCGCCGTGCGGTCCAAAGCGCCGGGC GCCACGGTGGCGCTAACCTTTCAGGATCCCTCGGGCGGTAGCCGCACAGTGCAAGTCACCCTC GGCAAGGCGGAGCAGTGA

>Rv1008 - Similar to E.coli protein YcfH TB.seq 1127087:1127878 MW:29066 >emb|AL123456|MTBH37RV: 1127087-1127881 , Rv1008 SEQ ID NO:37

TTGGTCGACGCCCACACCCATCTCGACGCGTGCGGTGCACGAGACGCCGATACGGTGCGGTC GCTCGTCGAGCGAGCCGCCGCGGCCGGCGTGACCGCGGTGGTCACCGTCGCCGACGACCTG GAGTCCGCGCGCTGGGTCACCCGCGCGGCCGAATGGGATCGGCGAGTCTATGCCGCGGTGGC

GTTGCACCCGACCCGCGCCGATGCGCTCACCGACGCTGCCCGTGCCGAGCTCGAGCGATTGG TTGCCCACCCCAGGGTGGTGGCCGTCGGTGAGACCGGAATCGACATGTACTGGCCGGGTCGC CTGGACGGGTGTGCGGAGCCGCACGTCCAGCGGGAGGCCTTTGCCTGGCATATCGATCTGGC CAAGCGGACCGGTAAACCGCTGATGATCCACAATCGTCAGGCCGACCGCGACGTGCTGGACGT GCTGCGGGCCGAGGGCGCGCCGGACACCGTGATCTTGCACTGCTTCTCGTCGGACGCGGCGA

TGGCCCGCACGTGTGTGGACGCCGGGTGGCTGCTCAGCCTGTCCGGGACGGTGAGCTTCCGT ACCGCCCGTGAACTACGGGAAGCCGTCCCGCTGATGCCGGTGGAGCAGCTTTTGGTGGAAACC GATGCACCGTATTTGACCCCGCATCCCCACCGGGGCTTGGCGAACGAACCGTACTGCCTGCCC TATACCGTGCGGGCGCTGGCTGAACTGGTCAATCGGCGCCCCGAAGAGGTGGCGCTCATCACC ACAAGCAACGCTCGCCGAGCTTATGGGCTAGGGTGGATGCGCCAATGA >Rv1009 - lipoprotein, similar to various other MTB proteins TB.seq 1128089:1129174 MW:38079 >emb|AL123456|MTBH37RV:1128089-1129177, Rv1009 SEQ ID NO:38

ATGTTGCGCCTGGTAGTCGGTGCGCTGCTGCTGGTGTTGGCGTTCGCCGGTGGCTATGCGGTC GCCGCATGCAAAACGGTGACGTTGACCGTCGACGGAACCGCGATGCGGGTGACCACGATGAAA TCGCGGGTGATCGACATCGTCGAAGAGAACGGGTTCTCAGTCGACGACCGCGACGACCTGTAT

CCCGCGGCCGGCGTGCAGGTCCATGACGCCGACACCATCGTGCTGCGGCGTAGCCGTCCGCT GCAGATCTCGCTGGATGGTCACGACGCTAAGCAGGTGTGGACGACCGCGTCGACGGTGGACG AGGCGCTGGCCCAACTCGCGATGACCGACACGGCGCCGGCCGCGGCTTCTCGCGCCAGCCGC GTCCCGCTGTCCGGGATGGCGCTACCGGTCGTCAGCGCCAAGACGGTGCAGCTCAACGACGG CGGGTTGGTGCGCACGGTGCACTTGCCGGCCCCCAATGTCGCGGGGCTGCTGAGTGCGGCCG

GCGTGCCGCTGTTGCAAAGCGACCACGTGGTGCCCGCCGCGACGGCCCCGATCGTCGAAGGC ATGCAGATCCAGGTGACCCGCAATCGGATCAAGAAGGTCACCGAGCGGCTGCCGCTGCCGCCG AACGCGCGTCGTGTCGAGGACCCGGAGATGAACATGAGCCGGGAGGTCGTCGAAGACCCGGG GGTTCCGGGGACCCAGGATGTGACGTTCGCGGTAGCTGAGGTCAACGGCGTCGAGACCGGCC GTTTGCCCGTCGCCAACGTCGTGGTGACCCCGGCCCACGAAGCCGTGGTGCGGGTGGGCACC

AAGCCCGGTACCGAGGTGCCCCCGGTGATCGACGGAAGCATCTGGGACGCGATCGCCGGCTG TGAGGCCGGTGGCAACTGGGCGATCAACACCGGCAACGGGTATTACGGTGGTGTGCAGTTTGA CCAGGGCACCTGGGAGGCCAACGGCGGGCTGCGGTATGCACCCCGCGCTGACCTCGCCACCC GCGAAGAGCAGATCGCCGTTGCCGAGGTGACCCGACTGCGTCAAGGTTGGGGCGCCTGGCCG GTATGTGCTGCACGAGCGGGTGCGCGCTGA

>Rv1010 ksgA 16S rRNA dimethyltransferase TB.seq 1129150:1130100 MW:34647 >emb|AL123456|MTBH37RV:1129150-1130103, ksgA SEQ ID NO:39 ATGTGCTGCACGAGCGGGTGCGCGCTGACCATCCGGCTGCTCGGGCGCACTGAGATCAGGCG GCTGGCCAAAGAGCTCGACTTTCGGCCGCGCAAATCTCTCGGACAGAACTTCGTGCACGACGC

CAACACGGTGCGACGGGTGGTTGCCGCCTCCGGGGTCAGCCGTTCCGACCTGGTTTTGGAGGT CGGGCCGGGCCTGGGATCGCTGACCCTGGCACTGCTCGACCGCGGCGCGACCGTCACCGCGG TCGAGATCGATCCACTACTGGCTTCTCGGCTGCAACAGACCGTGGCGGAGCACTCGCACAGCG AGGTTCACCGACTAACGGTGGTCAATCGCGACGTCCTGGCCCTGCGCCGGGAGGATCTAGCCG CGGCGCCGACCGCGGTGGTTGCCAATCTGCCGTACAACGTAGCGGTACCGGCGTTGTTGCATC

TGCTTGTCGAGTTCCCGTCGATCCGTGTCGTGACGGTGATGGTGCAGGCCGAGGTCGCCGAAC GGCTCGCCGCCGAGCCGGGCAGCAAAGAGTACGGCGTGCCCAGCGTTAAGCTGCGCTTCTTC GGGCGGGTTCGCCGCTGCGGCATGGTGTCGCCGACCGTTTTCTGGCCCATTCCGCGTGTCTAT TCCGGGCTGGTACGCATCGATCGATATGAGACCTCGCCCTGGCCCACCGACGACGCTTTTCGA CGGCGGGTATTCGAACTCGTGGACATCGCATTCGCGCAGCGGCGCAAGACTTCTCGCAACGCG

TTTGTGCAGTGGGCGGGCTCGGGAAGCGAGTCGGCGAATCGATTGTTGGCGGCCAGCATCGAC CCCGCCCGTCGCGGTGAGACGCTGTCCATCGACGACTTCGTGCGGCTGCTGCGACGGTCCGG CGGCTCCGACGAGGCCACCAGCACCGGCCGGGACGCCAGGGCGCCGGACATTTCGGGGCAC GCGTCGGCGAGCTGA

>Rv1011- Homology to E.coli protein YcbH TB.seq 1130189:1131106 MW:31350 >emb|AL123456|MTBH37RV:1130189-1131109, Rv1011 SEQ ID NO:40

GTGCCCACCGGGTCGGTCACCGTTCGGGTGCCCGGAAAGGTCAACCTCTATCTGGCGGTCGGC GATCGCCGCGAGGACGGCTATCACGAGCTGACCACGGTATTTCATGCCGTCTCGCTGGTCGAC GAGGTAACCGTTCGTAACGCTGATGTGCTCTCGCTCGAGTTGGTCGGCGAGGGGGCCGACCAG CTGCCGACCGACGAACGCAATCTCGCCTGGCAGGCGGCCGAGCTGATGGCCGAACACGTGGG CCGGGCGCCGGACGTCTCGATCATGATCGACAAATCCATTCCGGTCGCCGGCGGCATGGCCG

GTGGCAGCGCGGACGCTGCGGCGGTCCTGGTTGCGATGAACTCGTTGTGGGAACTCAATGTGC CCCGCCGCGACCTGCGCATGCTCGCCGCGCGGCTAGGCAGCGATGTGCCGTTTGCCCTGCAT GGTGGTACCGCGCTGGGGACGGGTCGCGGCGAGGAGTTGGCCACCGTGTTATCCCGCAACAC CTTCCACTGGGTCCTGGCGTTCGCCGACAGCGGGTTGCTCACCTCCGCGGTGTACAACGAGCT CGACCGGCTCAGGGAGGTGGGGGATCCGCCCCGGCTTGGTGAGCCCGGGCCGGTTCTGGCTG

CCTTAGCTGCGGGTGATCCGGATCAGCTGGCGCCGTTGCTGGGTAATGAAATGCAAGCGGCCG CGGTGAGCCTGGACCCGGCGCTGGCTCGTGCGTTACGCGCCGGTGTGGAGGCCGGCGCGCTC GCAGGCATCGTGTCCGGTTCGGGTCCCACGTGTGCCTTCCTGTGCACCTCGGCGAGCTCGGCG ATCGATGTCGGCGCGCAGCTGTCGGGGGCGGGAGTTTGTCGCACCGTTCGAGTCGCCACCGG GCCGGTACCCGGCGCCCGCGTGGTGTCTGCGCCGACCGAAGTGTGA

>Rv1106c - cholesterol dehydrogenase TB.seq 1232845: 1233954 MW:40743 >emb|AL123456|MTBH37RV:c1233954-1232842, Rv1106c SEQ ID NO:41 ATGCTTCGCCGCATGGGTGATGCATCGCTGACAACCGAGCTCGGCCGCGTTCTGGTCACCGGC GGCGCGGGCTTCGTGGGCGCCAACCTGGTGACCACCTTGCTGGACCGCGGGCACTGGGTGCG

TTCCTTCGACCGCGCGCCGTCGCTGTTGCCTGCGCATCCGCAACTGGAGGTGCTGCAAGGGGA CATCACCGACGCGGACGTCTGCGCCGCGGCCGTGGACGGCATCGACACGATCTTCCACACCG CAGCGATCATCGAGCTGATGGGCGGCGCGTCGGTCACCGACGAGTACCGCCAACGTAGCTTTG CGGTCAACGTCGGCGGCACCGAGAACCTGCTGCACGCCGGCCAGCGGGCCGGGGTGCAGCG GTTCGTCTACACGTCATCCAACAGTGTGGTGATGGGCGGCCAGAACATCGCCGGCGGTGACGA

GACGCTGCCCTATACCGACCGGTTCAACGACCTCTACACCGAGACCAAGGTGGTTGCCGAGCG ATTCGTGTTGGCCCAGAACGGTGTCGACGGCATGCTGACGTGCGCGATCCGGCCCAGCGGCAT CTGGGGAAACGGCGATCAGACGATGTTCCGCAAGCTGTTCGAAAGTGTGCTCAAGGGCCACGT CAAGGTGCTGGTCGGGCGCAAGTCGGCCCGGCTGGATAACTCTTACGTGCACAACCTGATTCA CGGTTTCATCTTGGCCGCTGCCCATCTGGTGCCGGACGGCACAGCGCCCGGGCAGGCTTACTT

CATCAACGACGCAGAGCCGATCAATATGTTCGAGTTCGCTCGGCCGGTGCTCGAGGCGTGCGG GCAGCGCTGGCCGAAGATGCGGATTTCCGGCCCCGCGGTCCGCTGGGTAATGACGGGGTGGC AGCGGCTGCACTTCCGGTTCGGATTCCCCGCGCCGCTGCTCGAGCCGCTGGCCGTCGAACGAC TGTACCTGGACAACTACTTTTCGATCGCTAAGGCACGCCGCGACCTGGGCTATGAGCCGCTGTT CACCACCCAGCAGGCGCTGACCGAATGCCTGCCGTACTACGTGAGTCTGTTTGAGCAGATGAA GAACGAGGCCCGGGCGGAAAAAACGGCCGCCACAGTCAAGCCGTAG

>Rv1110 lytB2 TB.seq 1236183:1237187 MW:36298 >emb|AL123456|MTBH37RV:1236183-1237190, lytB' SEQ ID NO:42

ATGGTTCCGACGGTCGACATGGGGATTCCCGGGGCTTCGGTATCGTCGCGATCGGTGGCCGAC CGTCCCAACCGTAAGCGGGTGCTGCTGGCCGAGCCGCGTGGCTACTGCGCTGGCGTGGATCG GGCCGTCGAAACGGTCGAACGCGCGCTTCAAAAACACGGCCCGCCTGTCTACGTGCGTCACGA

GATCGTGCATAACCGCCACGTGGTTGACACCCTGGCTAAGGCCGGTGCGGTTTTCGTCGAAGA GACCGAGCAGGTTCCCGAGGGAGCGATTGTGGTGTTCTCCGCGCACGGGGTCGCGCCTACGG TGCACGTCAGCGCCAGCGAGCGCAACCTGCAGGTCATTGACGCCACCTGCCCGCTGGTCACCA AGGTGCACAACGAGGCCAGGCGGTTCGCCCGGGACGACTACGACATCTTGCTGATCGGTCATG AGGGCCACGAGGAAGTCGTCGGTACTGCTGGGGAAGCTCCCGATCATGTGCAGCTGGTCGACG

GGGTGGACGCCGTCGACCAGGTGACCGTCCGTGACGAGGACAAAGTGGTTTGGCTGTCGCAG ACCACCCTGTCCGTCGATGAGACCATGGAGATTGTCGGGCGGTTGCGTCGGCGTTTCCCCAAG CTGCAGGATCCGCCCAGCGACGACATCTGCTATGCGACCCAGAATCGGCAGGTCGCGGTCAAG GCGATGGCGCCCGAGTGCGAGCTGGTCATCGTGGTCGGCTCGCGCAATTCGTCGAATTCGGTT CGGCTGGTCGAGGTGGCGCTGGGTGCCGGGGCGCGGGCCGCCCACCTGGTGGACTGGGCCG

ACGATATCGACTCGGCCTGGCTGGACGGCGTTACCACGGTCGGCGTTACGTCGGGGGCATCGG TCCCCGAGGTGCTGGTGCGCGGTGTGCTGGAGCGGCTGGCCGAATGCGGCTACGACATCGTG CAACCGGTGACAACGGCCAACGAGACGTTGGTGTTCGCATTGCCCCGGGAGCTCCGCTCACCT CGCTGA

>Rv1216c - TB.seq 1359473:1360144 MW:24863

>emb|AL123456|MTBH37RV:c1360144-1359470, Rv1216c SEQ ID NO:43

ATGCACATTGGGCTGAAGATATTCATATGGGGCGTGTTAGGACTCGTCGTTTTCGGCGCGCTCC TATTCGGGCCAGCCGGCACGTTCGACTATTGGCAGGCGTGGGTGTTCCTCGCCGCATTTGTGA GCACCACGATTGGCCCCACAATCTATCTGGCTCGCAACGATCCCGCGGCCCTTCAACGTCGCAT

GCGCAGCGGTCCGCTCGCGGAGGGCCGAACGATTCAGAAGTTCATCGTCATCGGCGCTTTTCT GGGGTTCTTCGCGATGATGGTGCTGAGCGCGTGCGACCATCGTTATGGTTGGTCGTCAGTGCC AGCCGCGGTGTGCGTGATCGGCGACGTCCTAGTGATGACGGGCCTTGGCATCGCCATGCTGGT GGTCATCCAGAACAGGTATGCCGCCTCGACGGTCAGGGTGGAGGCGGGCCAGATATTGGCCTC CGACGGTCTCTACAAAATTGTCCGACACCCGATGTACGCCGGGAACGTGGTCATGATGACAGG

CATACCGCTGGCACTGGGCTCTTACTGGGCGATGTTCATCCTCGTCCCCGGCACACTGGTGTTG GTGTTCCGCATCCTCGACGAGGAAAAACTACTGACGCAAGAACTCAGCGGGTACCGCGAATACC GGCAACTGGTGCGCTACCGGTTGGTGCCCTACGTGTGGTAG

>Rv1223 htrA TB.seq 1365810:1367456 MW:56547 >emb|AL123456|MTBH37RV: 1365810-1367459, htrA SEQ ID NO:44

GTGAGCCACTTGTCGCAGCGCATGGCGGGGTTGCTGCGAGTTCATGGCGAGTGGTCGCGATCC GTGGATACTAGGGTGGACACGGACAACGCGATGCCTGCACGTTTTAGCGCCCAGATTCAGAAT GAGGATGAGGTGACCTCCGACCAAGGCAACAACGGCGGCCCGAACGGCGGAGGCCGCCTGGC GCCGCGCCCGGTTTTTCGGCCACCGGTCGACCCGGCGTCGCGTCAAGCGTTCGGGCGTCCGT CCGGGGTCCAAGGGTCCTTTGTGGCCGAGCGTGTGCGCCCGCAGAAGTACCAGGACCAGTCT

GACTTCACACCGAACGATCAGCTTGCTGACCCGGTGCTTCAGGAGGCGTTCGGTCGTCCGTTC GCGGGCGCCGAATCGCTGCAGCGCCATCCCATCGATGCCGGAGCGCTGGCAGCTGAGAAAGA CGGTGCCGGCCCCGACGAGCCCGACGATCCGTGGCGCGACCCCGCGGCCGCGGCCGCGCTG GGGACGCCAGCGCTAGCCGCGCCGGCACCGCACGGTGCGCTGGCCGGCAGCGGCAAGCTGG GTGTGCGCGACGTGCTGTTTGGCGGCAAGGTGTCCTACTTGGCGCTGGGCATCTTGGTCGCTA

TCGCACTGGTGATCGGCGGCATCGGCGGTGTCATCGGCCGCAAGACCGCGGAAGTAGTCGAT GCGTTCACCACGTCGAAGGTGACCCTGTCGACCACTGGCAATGCCCAGGAACCGGCCGGCCG GTTCACCAAGGTGGCGGCCGCCGTGGCCGATTCGGTGGTGACCATTGAGTCGGTCAGCGACCA GGAGGGCATGCAAGGTTCCGGCGTCATCGTCGATGGCCGCGGCTACATCGTCACCAACAATCA CGTGATCTCTGAGGCGGCCAACAATCCCAGCCAGTTCAAGACGACCGTGGTGTTCAACGACGG

CAAGGAGGTGCCCGCCAATCTGGTGGGTCGTGACCCCAAGACCGACTTGGCCGTCCTCAAGGT CGACAACGTCGACAATCTGACCGTGGCCCGGCTCGGTGATTCCAGCAAGGTACGGGTCGGTGA CGAAGTCCTCGCGGTCGGCGCGCCCCTGGGGCTGCGCAGTACGGTGACCCAGGGCATTGTCA GCGCGCTACACCGCCCCGTTCCGTTGTCGGGCGAGGGCTCTGACACCGACACCGTCATTGACG CAATTCAGACCGACGCCTCGATCAACCACGGTAACTCCGGCGGTCCGCTAATCGACATGGATGC

CCAGGTGATTGGCATCAACACCGCCGGTAAGTCACTGTCGGATAGCGCCAGCGGGCTGGGCTT TGCGATCCCGGTCAACGAGATGAAATTGGTGGCAAATTCTCTGATCAAAGACGGAAAGATCGTG CATCCGACGTTGGGCATCAGCACCCGGTCAGTAAGCAACGCGATCGCGTCGGGCGCGCAGGT GGCCAATGTAAAGGCGGGAAGTCCCGCGCAGAAGGGCGGGATCTTGGAGAACGATGTGATCGT CAAGGTCGGTAACCGCGCGGTCGCCGACTCCGACGAGTTCGTCGTCGCCGTGCGCCAGTTGG

CTATCGGCCAGGACGCTCCGATAGAGGTGGTCCGCGAGGGTCGGCATGTGACGCTGACGGTG AAACCGGACCCCGATAGCACCTAG

>Rv1224 - TB.seq 1367461 :1367853 MW: 14083 >emb|AL123456|MTBH37RV:1367461-1367856, Rv1224 SEQ ID NO:45

GTGTTCGCCAACATCGGTTGGTGGGAAATGCTCGTCCTCGTCATGGTCGGGCTGGTGGTGCTT GGCCCGGAGCGGCTCCCGGGTGCCATCCGCTGGGCGGCAAGCGCTCTGCGGCAGGCGCGCG ACTATCTCAGCGGTGTGACCAGCCAGCTACGTGAGGACATTGGACCCGAATTCGATGATCTGCG GGGACATCTCGGTGAGCTGCAGAAGCTACGGGGAATGACTCCGCGGGCTGCGTTGACCAAGCA CCTACTGGATGGCGATGATTCCCTGTTCACCGGAGACTTCGACCGACCGACGCCGAAGAAACC GGATGCGGCGGGCTCGGCGGGGCCGGACGCTACTGAGCAGATCGGTGCGGGGCCCATCCCG TTTGACAGCGATGCCACCTAG

>Rv1229c mrp similar to MRP/NBP35 ATP-binding proteins TB.seq 1371778:1372947 MW:41064 >emb|AL123456|MTBH37RV:d 372947-1371775, mrp SEQ ID NO:46 ATGCCAAGCCGCCTACACTCGGCGGTGATGTCCGGAACTCGTGATGGCGACCTGAACGCGGCG ATACGCACCGCGCTGGGCAAGGTAATCGACCCCGAATTGCGGCGCCCCATCACCGAACTGGGG

ATGGTCAAAAGCATCGACACCGGCCCGGATGGGAGCGTGCACGTCGAGATCTACCTGACCATC GCCGGCTGCCCGAAGAAGTCCGAAATCACCGAGCGTGTCACCCGGGCGGTCGCCGACGTGCC AGGCACTTCGGCGGTGCGGGTCAGCTTGGACGTGATGAGCGACGAGCAGCGCACCGAGCTGC GTAAGCAGTTGCGTGGCGATACCCGCGAACCCGTCATCCCGTTCGCGCAACCCGATTCCTTGAC CCGGGTGTATGCCGTGGCTTCCGGTAAGGGCGGAGTCGGAAAGTCCACCGTCACGGTCAACCT

GGCCGCCGCGATGGCCGTCCGCGGCCTGTCGATCGGGGTGCTGGACGCTGATATCCACGGCC ACTCTATCCCCCGGATGATGGGCACCACCGACCGGCCTACCCAGGTTGAGTCGATGATCCTGC CGCCGATCGCCCACCAGGTGAAGGTCATCTCGATAGCCCAGTTCACCCAGGGCAACACCCCGG TGGTGTGGCGCGGGCCGATGCTGCACCGGGCGTTGCAGCAGTTTCTGGCCGACGTGTACTGG GGGGATCTGGACGTGCTGCTGCTGGACTTGCCGCCCGGAACCGGCGACGTCGCCATCTCGGT

GGCTCAACTGATCCCCAACGCCGAACTCCTGGTGGTCACCACCCCGCAGCTGGCCGCCGCGGA GGTGGCCGAACGGGCCGGCAGCATCGCGCTGCAAACCCGCCAACGCATCGTCGGCGTCGTGG AGAACATGTCGGGGCTCACGCTGCCGGACGGCACCACGATGCAGGTGTTCGGCGAGGGCGGT GGCCGGCTGGTCGCCGAGCGGTTGTCGCGTGCGGTCGGCGCCGACGTGCCGCTGCTGGGTCA GATCCCGCTGGACCCCGCACTGGTGGCCGCCGGCGATTCGGGCGTACCGCTCGTGTTGAGCT

CGCCGGACTCGGCGATCGGCAAGGAACTGCATAGCATCGCCGACGGCTTGTCGACTCGACGAC GCGGATTGGCGGGCATGTCGCTGGGGTTGGACCCGACACGACGCTAG

>Rv1239c corA magnesium and cobalt transport protein TB.seq 1381943:1383040 MW:41470 >emb|AL123456|MTBH37RV:c1383040-1381940, corA SEQ ID NO:47

GTGTTCCCAGGGTTTGACGCATTGCCCGAAGTGCTGCGACCGGTCGCGCGACCCCAGCCGCCG AACGCACACCCCGTTGCCCAGCCACCGGCCCAAGCCTTGGTCGACTGCGGTGTCTACGTCTGC GGCCAGCGACTGCCCGGCAAGTACACCTACGCCGCCGCGCTGCGCGAGGTGCGCGAGATCGA ACTGACCGGGCAGGAGGCGTTCGTCTGGATCGGGCTGCACGAGCCCGATGAAAACCAGATGCA GGACGTAGCAGACGTTTTCGGGTTGCACCCGTTAGCCGTTGAGGACGCCGTGCACGCGCACCA

GCGACCCAAGTTGGAGCGCTACGACGAGACGCTGTTCCTCGTCCTCAAGACCGTCAACTACGT CCCGCACGAATCGGTGGTACTGGCCCGCGAGATCGTCAAAACCGGCGAGATCATGATCTTCGT CGGCAAGGATTTCGTGGTCACCGTCCGCCACGGCGAACACGGCGGGTTATCCGAGGTGCGTAA GCGGATGGATGCCGACCCCGAACATTTGCGGTTGGGACCGTATGCGGTGATGCACGCGATCGC CGACTACGTGGTCGACCACTACCTCGAGGTGACCAATCTCATGGAGACCGATATCGACAGCATC GAGGAAGTAGCGTTCGCGCCGGGCCGCAAGCTCGACATCGAACCGATCTATCTGCTCAAGCGG GAAGTGGTCGAGTTGCGCCGGTGCGTGAATCCGCTATCGACCGCATTCCAGCGCATGCAGACC

GAGAGCAAAGACCTCATTTCGAAAGAAGTGCGGCGCTACCTGCGCGACGTCGCCGACCACCAG ACCGAGGCCGCCGACCAGATCGCCAGCTACGACGACATGCTCAACTCGCTGGTGCAGGCCGC GCTCGCCCGGGTCGGCATGCAGCAAAACATGGACATGCGCAAGATATCCGCGTGGGCAGGTAT CATCGCGGTCCCCACCATGATCGCGGGCATCTATGGCATGAACTTTCACTTCATGCCCGAGCTG GACTCCAGGTGGGGTTACCCGACAGTGATCGGCGGGATGGTCCTTATCTGTCTGTTCCTCTACC

ACGTCTTCCGCAACAGAAACTGGCTCTAG

>Rv1279 - TB.seq 1430060:1431643 MW:57332 >emb|AL123456|MTBH37RV:1430060-1431646, Rv1279 SEQ ID NO:48 ATGGACACTCAGAGCGACTACGTCGTGGTCGGTACCGGCTCAGCCGGGGCGGTTGTGGCCAG

CCGGCTTAGCACCGATCCGGCCACGACGGTGGTGGCCCTGGAGGCGGGGCCGCGTGACAAGA ACAGATTCATCGGCGTCCCAGCGGCGTTTTCCAAGCTGTTCCGCAGCGAGATCGACTGGGATTA CCTAACCGAACCGCAGCCGGAGCTCGACGGCCGCGAAATCTATTGGCCTCGTGGCAAGGTGCT CGGTGGCTCGTCGTCCATGAACGCAATGATGTGGGTGCGTGGATTCGCATCAGACTACGATGA GTGGGCCGCGCGAGCCGGTCCGCGGTGGTCGTACGCCGACGTGCTCGGCTACTTTCGCCGCA

TCGAGAACGTCACCGCTGCCTGGCACTTTGTCAGCGGTGACGACAGCGGAGTAACCGGTCCGT TGCATATTTCCCGGCAACGCAGCCCAAGATCGGTGACCGCAGCGTGGCTGGCAGCCGCACGTG AGTGCGGATTTGCCGCTGCGCGGCCGAATTCCCCTCGACCGGAAGGCTTTTGCGAGACCGTCG TCACCCAGCGCCGCGGTGCTCGATTCAGTACTGCCGACGCCTATCTGAAGCCCGCGATGCGCC GTAAAAACCTCCGTGTGCTTACCGGCGCCACTGCTACCCGGGTGGTCATCGACGGCGACCGGG

CCGTCGGCGTGGAATACCAAAGCGACGGTCAAACCCGCATCGTCTACGCCCGCCGCGAGGTG GTGCTCTGCGCTGGTGCCGTCAACAGCCCTCAGCTGCTGATGCTCTCCGGCATCGGCGACCGC GACCACCTCGCCGAACACGACATCGACACCGTTTACCACGCGCCCGAGGTCGGGTGCAACCTG CTCGATCATCTCGTCACGGTGCTGGGTTTCGACGTCGAAAAGGACAGCTTGTTTGCCGCCGAGA AGCCCGGCCAGTTGATCAGCTACTTACTGCGACGCCGCGGCATGCTCACCTCCAACGTCGGCG

AGGCGTACGGATTTGTCCGCAGCCGACCCGAACTGAAGCTGCCCGATTTGGAGTTGATTTTTGC CCCGGCGCCGTTTTACGACGAAGCGCTGGTTCCACCGGCTGGTCACGGTGTGGTATTCGGCCC GATTCTGGTCGCGCCGCAAAGCCGTGGCCAGATCACGCTGCGGTCCGCCGATCCGCATGCCAA GCCTGTCATCGAACCGCGTTACCTGTCCGATCTCGGTGGCGTAGACCGGGCCGCCATGATGGC GGGCCTGCGGATATGCGCGCGGATCGCGCAGGCCCGCCCGCTCAGAGATCTCCTTGGGTCCA

TCGCGCGACCGCGCAACAGCACCGAGCTGGACGAGGCCACTCTCGAGTTGGCGCTGGCCACT TGTTCGCACACCCTGTACCACCCGATGGGCACCTGCCGCATGGGCAGCGACGAGGCCAGCGT GGTGGATCCGCAGCTGCGGGTCCGCGGTGTCGACGGACTCCGCGTCGCCGACGCGTCGGTGA

TGCCCAGCACGGTTCGTGGGCATACGCATGCGCCGTCGGTGCTGATCGGGGAGAAGGCCGCC

GACTTAATCCGCAGCTGA

>Rv1294 thrA homoserine dehydrogenase TB.seq 1449373:1450695 MW:45522

>emb|AL123456|MTBH37RV:1449373-1450698, thrA SEQ ID NO:49

GTGCCCGGTGACGAAAAGCCGGTCGGCGTAGCGGTACTCGGTTTGGGCAACGTCGGCAGCGA GGTTGTCCGCATCATCGAGAACAGCGCCGAGGATCTCGCGGCTCGTGTCGGTGCCCCATTGGT CCTGCGGGGCATCGGCGTGCGCCGCGTGACGACCGATCGCGGCGTGCCGATCGAATTGTTGA CCGACGACATTGAAGAGCTCGTGGCCCGCGAGGATGTCGATATCGTGGTGGAAGTGATGGGGC

CGGTGGAACCGTCGCGCAAGGCGATCCTGGGCGCCCTTGAGCGCGGCAAGTCCGTCGTTACG GCGAACAAGGCTTTACTCGCCACCTCCACCGGCGAATTGGCACAGGCCGCCGAAAGCGCCCAT GTTGATCTGTATTTCGAGGCGGCCGTGGCGGGCGCCATTCCGGTCATCCGTCCGCTCACCCAG TCGCTGGCCGGCGACACGGTGCTGCGAGTGGCCGGGATCGTCAACGGCACCACCAACTACATC CTCTCGGCGATGGACAGCACCGGCGCTGACTATGCCAGCGCCCTGGCCGACGCAAGTGCGCT

GGGCTATGCGGAGGCTGATCCCACCGCAGACGTCGAAGGCTACGACGCCGCGGCCAAGGCAG CGATCCTGGCATCCATTGCCTTCCACACCCGGGTGACCGCAGACGACGTGTATCGCGAAGGCA TCACCAAGGTCACTCCGGCCGACTTCGGATCCGCGCACGCGCTGGGTTGCACCATCAAACTGC TGTCGATCTGTGAGCGCATAACCACCGACGAAGGTTCGCAGCGGGTATCGGCCCGCGTCTATC CGGCCCTGGTACCTCTGTCGCATCCGCTTGCCGCGGTCAACGGCGCGTTCAATGCCGTGGTGG

TCGAGGCCGAGGCCGCGGGCCGGCTGATGTTCTACGGCCAGGGCGCGGGCGGCGCGCCGAC CGCCTCTGCGGTGACCGGTGACCTAGTGATGGCCGCCCGCAACCGGGTACTCGGCAGCCGCG GCCCCCGTGAGTCTAAATACGCTCAACTTCCGGTGGCACCAATGGGTTTCATTGAAACGCGCTA TTACGTCAGCATGAACGTCGCCGACAAGCCGGGCGTCTTGTCCGCGGTGGCGGCGGAATTCGC CAAACGCGAGGTGAGCATCGCCGAGGTGCGCCAGGAGGGCGTTGTGGACGAAGGTGGTCGAC

GGGTGGGAGCCCGAATCGTGGTGGTCACGCACCTCGCCACTGACGCCGCACTCTCGGAAACC GTTGATGCACTGGACGACTTGGATGTCGTGCAGGGTGTGTCCAGCGTGATACGACTGGAAGGA ACCGGCTTATGA

>Rv1323 fadA4 acetyl-CoA C-acetyltransferase (aka thiL) TB.seq 1485860:1487026 MW:40049

>emb|AL123456|MTBH37RV:1485860-1487029, fadA4 SEQ ID NO:50

GTGATTGTTGCTGGCGCGCGTACACCCATCGGCAAGTTGATGGGCTCCCTGAAGGATTTCAGCG CCAGCGAGCTGGGTGCCATCGCCATTAAGGGCGCCCTGGAGAAGGCCAACGTGCCGGCGTCC TTGGTCGAGTACGTGATCATGGGCCAGGTGTTGACCGCGGGTGCCGGGCAAATGCCCGCACG GCAGGCGGCAGTGGCGGCCGGCATCGGTTGGGATGTCCCTGCGCTGACGATCAACAAGATGT

GCCTGTCCGGCATCGACGCAATCGCGCTGGCTGATCAACTCATTCGGGCCAGAGAGTTCGACG TGGTGGTGGCCGGCGGTCAGGAGTCGATGACGAAGGCGCCCCACCTGTTGATGAATAGCCGGT CGGGTTACAAGTACGGCGACGTTACGGTTTTGGACCACATGGCCTACGACGGTCTGCACGACG TGTTCACCGATCAGCCGATGGGCGCGCTCACCGAGCAACGCAACGACGTCGACATGTTCACCC GCTCCGAACAGGACGAGTACGCGGCTGCGTCCCACCAAAAGGCGGCCGCGGCATGGAAGGAC GGCGTATTCGCCGACGAGGTGATCCCGGTGAACATCCCGCAGCGCACGGGCGATCCACTGCA GTTCACCGAGGACGAGGGGATCCGCGCCAACACCACCGCCGCCGCGCTGGCCGGTCTGAAGC

CGGCGTTCCGTGGCGACGGCACCATCACCGCCGGGTCGGCGTCACAGATCTCCGACGGTGCG GCCGCGGTGGTGGTCATGAACCAGGAAAAGGCCCAGGAACTGGGGCTGACCTGGCTAGCCGA GATCGGCGCCCACGGTGTGGTGGCCGGGCCGGATTCCACACTGCAATCGCAGCCGGCCAACG CGATCAACAAGGCGCTGGATCGCGAGGGCATCTCGGTGGACCAGCTCGACGTGGTGGAGATCA ACGAGGCGTTCGCTGCGGTGGCATTGGCCTCGATACGCGAACTCGGGCTGAACCCCCAGATCG

TCAACGTCAACGGTGGTGCGATTGCCGTCGGGCATCCCCTCGGCATGTCAGGGACGCGAATCA CGCTACATGCGGCGCTGCAGTTGGCACGCCGGGGATCGGGCGTCGGGGTTGCCGCATTGTGC GGGGCTGGCGGGCAGGGCGACGCACTGATATTGCGGGCCGGATAG

>Rv1389 gmk putative guanylate kinase TB.seq 1564399:1565022 MW:22064

>emb|AL123456|MTBH37RV: 1564399-1565025, gmk SEQ ID N0:51

GTGAGCGTCGGCGAGGGACCGGACACCAAGCCCACCGCGCGTGGCCAACCGGCGGCAGTGG GACGTGTGGTGGTGCTGTCCGGTCCTTCCGCGGTCGGCAAATCCACGGTGGTTCGGTGTCTGC GCGAGCGGATCCCGAATCTGCATTTCAGTGTCTCGGCCACGACGCGGGCGCCACGCCCGGGC GAGGTCGACGGTGTCGACTACCACTTCATCGACCCCACCCGCTTTCAGCAGCTCATCGACCAG

GGTGAGTTGCTGGAATGGGCAGAAATCCACGGCGGCCTGCACCGGTCGGGCACTTTGGCCCA GCCGGTGCGGGCGGCCGCGGCGACTGGTGTGCCGGTGCTTATCGAGGTTGACCTGGCCGGGG CCAGGGCGATCAAGAAGACGATGCCCGAGGCTGTCACCGTGTTTCTGGCGCCACCTAGCTGGC AGGATCTTCAGGCCAGACTGATTGGCCGCGGCACCGAAACAGCTGACGTTATCCAACGCCGCC TGGACACCGCGCGGATCGAATTGGCAGCGCAGGGCGACTTTGACAAGGTCGTGGTGAACAGGC

GATTAGAGTCTGCGTGTGCGGAATTGGTATCCTTGCTGGTGGGAACGGCACCGGGCTCCCCGT GA

>Rv1407 fmu similar to Fmu protein TB.seq 1583099:1584469 MW:48494 >emb|AL123456|MTBH37RV:1583099-1584472, fmu SEQ ID NO:52

ATGACCCCTAGATCGCGTGGGCCGCGCCGCCGGCCGCTGGACCCGGCGCGTCGTGCGGCCTT CGAGACGCTGCGGGCGGTTAGTGCGCGCGACGCCTACGCGAACCTGGTGTTGCCCGCGCTGC TGGCCCAACGCGGTATCGGCGGTCGCGACGCCGCGTTCGCCACCGAGCTGACATACGGCACC TGCCGAGCCCGCGGCCTGCTCGACGCGGTCATCGGTGCGGCCGCCGAGCGTTCGCCGCAGGC GATCGATCCGGTGCTGCTAGACCTGTTGCGGCTCGGCACCTACCAATTGCTGCGCACGCGGGT

CGACGCACACGCCGCAGTGTCGACCACCGTCGAGCAGGCCGGAATCGAATTCGATTCGGCGC GAGCAGGTTTCGTCAACGGTGTACTACGAACGATCGCCGGCCGAGACGAGCGGTCCTGGGTTG GCGAACTCGCTCCTGATGCGCAGAACGATCCGATCGGGCATGCCGCGTTCGTGCATGCGCATC CCCGATGGATCGCCCAGGCCTTTGCTGACGCGTTGGGCGCGGCGGTCGGGGAGCTCGAGGCA GTTTTGGCCAGCGACGACGAACGGCCAGCGGTGCACCTGGCGGCACGCCCCGGGGTGCTGAC CGCCGGCGAACTGGCCCGCGCGGTGCGCGGAACCGTCGGTCGGTATTCGCCGTTTGCGGTGT ATCTGCCGCGCGGTGACCCGGGGCGACTGGCGCCGGTGCGCGACGGCCAAGCGCTGGTCCA

GGACGAGGGCAGCCAGTTAGTCGCCCGAGCATTGACCCTGGCGCCAGTCGACGGCGATACCG GACGGTGGCTGGACCTGTGTGCCGGACCGGGCGGCAAGACCGCGCTGTTGGCCGGGCTGGGT TTGCAGTGCGCAGCCCGGGTGACCGCGGTGGAACCCTCGCCACACCGCGCGGACCTGGTAGC ACAGAACACCCGCGGGCTGCCGGTTGAGCTCTTGCGTGTCGACGGGCGGCACACCGACCTCG ACCCGGGTTTCGACCGGGTGCTGGTGGATGCGCCCTGCACCGGGCTGGGCGCGTTACGCCGT

CGGCCGGAGGCCCGTTGGCGTCGTCAGCCGGCGGACGTAGCGGCACTGGCCAAGCTACAACG CGAGTTGTTGAGCGCCGCCATCGCGCTGACTCGGCCCGGCGGTGTCGTGCTCTATGCCACATG CTCGCCGCACCTGGCCGAGACTGTGGGTGCTGTCGCCGACGCGCTACGCCGACATCCGGTTCA CGCGCTCGATACCCGCCCACTGTTCGAGCCGGTGATCGCGGGGCTGGGGGAGGGGCCCCACG TTCAGCTGTGGCCGCACCGGCACGGTACCGACGCCATGTTCGCCGCGGCGTTGCGCCGCCTG

ACGTGA

>Rv1409 ribG riboflavin biosynthesis TB.seq 1585192:1586208 MW:35367 >emb|AL123456|MTBH37RV:1585192-1586211 , ribG SEQ ID NO:53 ATGAACGTGGAGCAGGTCAAGAGCATCGACGAGGCTATGGGTCTCGCCATCGAGCACTCCTAC

CAGGTCAAAGGCACGACTTATCCAAAACCCCCAGTGGGGGCCGTCATTGTGGATCCCAACGGT CGGATCGTCGGCGCCGGCGGCACCGAGCCGGCCGGTGGCGATCATGCCGAGGTGGTGGCGC TGCGCCGGGCCGGCGGATTGGCTGCCGGCGCCATCGTGGTGGTCACCATGGAACCCTGTAAC CACTACGGCAAGACTCCGCCATGCGTGAACGCTCTGATCGAAGCCAGGGTGGGGACGGTGGTC TACGCCGTCGCCGACCCGAACGGGATCGCTGGGGGTGGCGCGGGCCGGCTGTCAGCAGCGG

GCCTACAGGTGCGGTCCGGGGTGTTGGCTGAACAGGTGGCGGCCGGACCGCTGCGGGAGTGG CTCCACAAGCAACGCACCGGTCTGCCGCATGTCACCTGGAAGTACGCCACCAGCATCGACGGC CGCAGCGCCGCCGCCGACGGCTCCAGCCAGTGGATCTCCAGCGAGGCCGCACGCCTGGATCT GCATCGCCGCCGCGCCATCGCCGACGCGATCTTGGTCGGCACCGGCACCGTCCTCGCCGACG ACCCGGCCCTGACCGCGCGGCTGGCCGACGGCTCGCTGGCGCCGCAGCAGCCGCTGCGCGT

GGTGGTGGGCAAGCGCGACATACCGCCGGAAGCACGGGTCCTCAACGACGAGGCACGCACCA TGATGATCCGCACCCACGAACCTATGGAGGTGCTCAGGGCGTTGTCGGATCGCACCGACGTGC TGCTGGAAGGAGGTCCCACCCTCGCCGGCGCCTTCCTACGAGCGGGTGCGATCAACCGGATCC TGGCCTACGTCGCACCGATCCTGTTGGGCGGTCCGGTTACCGCGGTCGATGACGTCGGGGTGT CCAACATCACCAACGCGTTGCGTTGGCAGTTCGACAGCGTCGAAAAGGTCGGACCGGATCTGTT

GCTGAGCTTGGTGGCTCGTTAG >Rv1440 secG TB.seq 1617715:1618065 MW:12140 >emb|AL123456|MTBH37RV:1617715-1618068, secG SEQ ID NO:54

GTGGCAGGCGTGACAGCCGCGGTCAGTGCACGCCTCAAAGCCGATGAGGCGCGACGGCCTGG GTTCTACGCGGCAGGCAGCGGTCCGCTGCCGCAGGTTCGGGGGAGTACGCTACCCGTCATGG AATTGGCCCTGCAGATCACGCTGATCGTCACGAGCGTGCTGGTGGTGTTGTTAGTACTGCTGCA

CCGGGCCAAGGGTGGCGGGCTATCGACACTGTTCGGCGGTGGTGTGCAGTCAAGCCTGTCCG GCTCGACGGTGGTGGAGAAGAACCTGGACCGGTTGACGCTGTTCGTTACCGGCATCTGGCTGG TGTCCATCATCGGCGTGGCGTTGCTCATCAAATACCGCTAG

>Rv1484 inhA TB.seq 1674200: 1675006 MW:28529

>emb|AL123456|MTBH37RV: 1674200-1675009, inhA SEQ ID NO:55

ATGACAGGACTGCTGGACGGCAAACGGATTCTGGTTAGCGGAATCATCACCGACTCGTCGATCG CGTTTCACATCGCACGGGTAGCCCAGGAGCAGGGCGCCCAGCTGGTGCTCACCGGGTTCGAC CGGCTGCGGCTGATTCAGCGCATCACCGACCGGCTGCCGGCAAAGGCCCCGCTGCTCGAACT CGACGTGCAAAACGAGGAGCACCTGGCCAGCTTGGCCGGCCGGGTGACCGAGGCGATCGGGG

CGGGCAACAAGCTCGACGGGGTGGTGCATTCGATTGGGTTCATGCCGCAGACCGGGATGGGC ATCAACCCGTTCTTCGACGCGCCCTACGCGGATGTGTCCAAGGGCATCCACATCTCGGCGTATT CGTATGCTTCGATGGCCAAGGCGCTGCTGCCGATCATGAACCCCGGAGGTTCCATCGTCGGCA TGGACTTCGACCCGAGCCGGGCGATGCCGGCCTACAACTGGATGACGGTCGCCAAGAGCGCG TTGGAGTCGGTCAACAGGTTCGTGGCGCGCGAGGCCGGCAAGTACGGTGTGCGTTCGAATCTC

GTTGCCGCAGGCCCTATCCGGACGCTGGCGATGAGTGCGATCGTCGGCGGTGCGCTCGGCGA GGAGGCCGGCGCCCAGATCCAGCTGCTCGAGGAGGGCTGGGATCAGCGCGCTCCGATCGGCT GGAACATGAAGGATGCGACGCCGGTCGCCAAGACGGTGTGCGCGCTGCTGTCTGACTGGCTG CCGGCGACCACGGGTGACATCATCTACGCCGACGGCGGCGCGCACACCCAATTGCTCTAG

>Rv1617 pykA pyruvate kinase TB.seq 1816187:1817602 MW:50668

>emb|AL123456|MTBH37RV:1816187-1817605, pykA SEQ ID NO:56

GTGACGAGACGCGGGAAAATCGTCTGCACTCTCGGGCCGGCCACCCAGCGGGACGACCTGGT CAGAGCGCTGGTCGAGGCCGGAATGGACGTCGCCCGAATGAACTTCAGCCACGGCGACTACGA CGATCACAAGGTCGCCTATGAGCGGGTCCGGGTAGCCTCCGACGCCACCGGGCGCGCGGTCG

GCGTGCTCGCCGACCTGCAGGGCCCGAAGATCAGGTTGGGACGCTTCGCCTCCGGGGCCACC CACTGGGCCGAAGGCGAAACCGTCCGGATCACCGTGGGCGCCTGCGAGGGCAGCCACGATCG GGTGTCCACCACCTACAAGCGGCTAGCCCAGGACGCGGTGGCCGGTGACCGGGTGCTGGTCG ACGACGGCAAAGTCGCATTGGTGGTCGACGCCGTCGAGGGCGACGACGTGGTCTGCACCGTC GTCGAAGGCGGCCCGGTCAGCGACAACAAGGGCATCTCGTTGCCCGGAATGAACGTGACCGC

GCCGGCCCTGTCGGAGAAGGACATCGAGGATCTCACGTTCGCGCTGAACCTCGGCGTCGACAT GGTGGCGCTTTCCTTCGTCCGCTCCCCGGCCGATGTCGAACTGGTCCACGAGGTGATGGATCG GATCGGGCGACGGGTGCCGGTGATCGCCAAGCTGGAGAAGCCGGAAGCCATCGACAATCTCG AAGCGATCGTGCTGGCGTTCGACGCCGTCATGGTCGCTCGGGGCGACCTAGGTGTTGAGCTGC CGCTCGAAGAGGTCCCGCTGGTACAGAAGCGAGCCATCCAGATGGCCCGGGAGAACGCCAAG CCGGTCATTGTGGCGACCCAGATGCTCGACTCGATGATCGAGAACTCGCGGCCGACCCGAGCT GAGGCCTCCGACGTCGCCAACGCGGTGCTCGATGGCGCCGACGCGCTGATGCTGTCCGGGGA

AACCTCGGTAGGGAAGTACCCCCTTGCTGCGGTCCGGACAATGTCGCGCATCATCTGCGCGGT CGAGGAGAACTCCACGGCCGCACCGCCGTTGACACACATTCCCCGGACCAAGCGTGGGGTCAT CTCGTATGCGGCCCGTGACATCGGCGAACGACTCGACGCCAAGGCCTTGGTGGCCTTCACTCA GTCCGGTGATACCGTGCGGCGACTGGCCCGCCTGCATACCCCGCTGCCGCTGCTGGCCTTCAC CGCGTGGCCCGAGGTGCGCAGCCAACTGGCGATGACCTGGGGCACCGAGACGTTCATCGTGC

CGAAGATGCAGTCCACCGATGGCATGATCCGCCAGGTCGACAAATCGCTGCTCGAACTCGCCC GCTACAAGCGTGGTGACTTGGTGGTCATCGTCGCGGGTGCGCCGCCAGGCACAGTGGGTTCGA CCAACCTGATCCACGTGCACCGGATCGGGGAAGATGACGTCTAG

>Rv1630 rpsA 30S ribosomal protein S1 TB.seq 1833540:1834982 MW:53203

>emb|AL123456|MTBH37RV: 1833540-1834985, rpsA SEQ ID NO:57

ATGCCGAGTCCCACCGTCACCTCGCCGCAAGTAGCCGTCAACGACATAGGCTCTAGCGAGGAC TTTCTCGCCGCAATAGACAAAACGATCAAGTACTTCAACGATGGCGACATCGTCGAAGGCACCA TCGTCAAAGTGGACCGGGACGAGGTGCTCCTCGACATCGGCTACAAGACCGAAGGCGTGATCC CCGCCCGCGAACTGTCCATCAAGCACGACGTCGACCCCAACGAGGTCGTTTCCGTCGGTGACG

AGGTCGAAGCCCTGGTGCTCACCAAGGAGGACAAAGAGGGCCGGCTCATCCTCTCCAAGAAAC GCGCGCAGTACGAGCGTGCCTGGGGCACCATCGAGGCGCTCAAGGAGAAGGACGAGGCCGTC AAGGGCACGGTCATCGAGGTCGTCAAGGGTGGCCTGATCCTCGACATCGGGCTGCGCGGTTTC CTGCCCGCCTCGCTGGTGGAGATGCGCCGGGTGCGCGACCTGCAGCCCTACATCGGCAAGGA GATCGAGGCCAAGATCATCGAGCTGGACAAGAACCGCAACAACGTGGTGCTGTCCCGTCGCGC

CTGGCTGGAGCAGACCCAGTCCGAGGTGCGCAGCGAGTTCCTGAATAACTTGCAAAAAGGCAC CATCCGAAAGGGTGTCGTGTCCTCGATCGTCAACTTCGGCGCGTTCGTCGATCTCGGCGGTGT GGACGGTCTGGTGCATGTCTCCGAGCTATCGTGGAAGCACATCGACCACCCGTCCGAGGTGGT CCAGGTTGGTGACGAGGTCACCGTCGAGGTGCTCGACGTCGACATGGACCGTGAGCGGGTTTC GTTGTCACTCAAGGCGACTCAGGAAGACCCGTGGCGGCACTTCGCCCGCACTCACGCGATCGG

GCAGATCGTGCCGGGCAAGGTCACCAAGTTGGTTCCGTTCGGTGCATTCGTCCGCGTCGAGGA GGGTATCGAGGGCCTGGTGCACATCTCCGAGCTGGCCGAGCGTCACGTCGAGGTGCCCGATC AGGTGGTTGCCGTCGGCGACGACGCGATGGTCAAGGTCATCGACATCGACCTGGAGCGCCGTC GGATCTCGTTGTCGCTCAAGCAAGCCAATGAGGACTACACCGAGGAGTTCGACCCGGCGAAGT ACGGCATGGCCGACAGTTACGACGAGCAGGGCAACTACATCTTCCCCGAGGGCTTCGATGCCG

AAACCAACGAATGGCTTGAGGGATTCGAAAAGCAGCGCGCCGAATGGGAAGCTCGGTACGCCG AGGCCGAGCGCCGGCACAAGATGCACACCGCGCAGATGGAGAAGTTCGCCGCCGCCGAGGCG GCTGGACGCGGCGCGGACGATCAGTCGTCGGCCAGTAGCGCACCGTCGGAAAAGACCGCGGG

TGGATCACTGGCCAGCGACGCCCAGCTGGCGGCCCTGCGGGAAAAACTCGCCGGCAGCGCTT

GA

>Rv1631 - TB.seq 1835011 :1836231 MW:44669

>emb|AL123456|MTBH37RV:1835011-1836234, Rv1631 SEQ ID NO:58

ATGCTGCGCATCGGGCTGACCGGCGGCATTGGCGCCGGGAAGTCGTTGCTGTCCACGACGTTC TCGCAATGCGGCGGAATCGTTGTCGACGGCGATGTGTTGGCGCGTGAAGTGGTCCAGCCGGGC ACCGAGGGGCTGGCCTCGCTGGTCGACGCGTTCGGTCGCGACATCCTGCTTGCAGACGGAGC GCTGGACCGGCAGGCGTTGGCGGCCAAGGCGTTTCGAGATGACGAGTCGCGCGGTGTGCTCA

ACGGAATCGTGCACCCGCTGGTCGCCCGGCGCCGATCCGAGATCATCGCGGCGGTTTCGGGG GACGCGGTTGTGGTCGAAGATATTCCACTGCTGGTGGAATCCGGGATGGCGCCATTGTTTCCGC TGGTGGTGGTGGTGCACGCCGACGTCGAGCTACGGGTGCGACGGCTGGTCGAGCAACGCGGC ATGGCCGAAGCCGACGCCCGGGCTAGGATCGCTGCGCAGGCCAGCGACCAGCAGCGTCGTGC CGTCGCCGACGTCTGGCTGGACAACTCGGGCAGCCCAGAGGATTTGGTGCGGCGGGCCCGCG

ACGTCTGGAACACGCGCGTCCAGCCCTTCGCGCACAACCTGGCCCAACGTCAGATTGCGCGCG CGCCGGCTAGGTTGGTGCCGGCGGATCCAAGCTGGCCGGATCAGGCGCGGCGCATCGTCAAC CGGCTAAAGATCGCGTGCGGGCATAAGGCCTTGCGAGTTGACCACATTGGGTCAACCGCCGTG TCGGGCTTCCCCGATTTTCTAGCCAAGGATGTCATCGACATCCAGGTCACCGTCGAATCACTTG ACGTGGCCGACGAGCTGGCCGAGCCCTTGCTGGCCGCCGGCTACCCACGCCTCGAGCACATC

ACCCAGGACACCGAAAAGACCGACGCTCGCAGCACCGTCGGCCGCTACGACCACACCGACAGT GCCGCTCTGTGGCACAAGCGCGTGCACGCCTCGGCGGATCCCGGTCGGCCGACCAACGTGCA CCTGCGGGTGCACGGCTGGCCCAACCAACAGTTCGCCCTGCTGTTCGTCGACTGGCTGGCGGC CAATCCCGGCGCGAGAGAAGACTATTTGACGGTCAAGTGTGACGCCGACAGGCGCGCCGACG GTGAGCTCGCGCGCTACGTCACCGCCAAGGAGCCGTGGTTCCTGGATGCCTACCAGCGGGCAT

GGGAGTGGGCGGATGCGGTGCACTGGCGTCCCTGA

>Rv1706c - TB.seq 1932695:1933876 MW:39779 >emb|AL123456|MTBH37RV:d 933876-1932692, PPE SEQ ID NO:59 ATGACCCTCGATGTCCCGGTCAACCAGGGGCATGTCCCCCCGGGCAGCGTCGCCTGCTGCCTT

GTTGGGGTCACCGCCGTTGCTGACGGCATCGCCGGGCATTCCCTGTCCAACTTTGGGGCGTTA CCTCCCGAGATCAATTCGGGTCGTATGTATAGCGGTCCGGGATCCGGGCCACTGATGGCTGCC GCGGCGGCCTGGGACGGGCTGGCCGCAGAGTTGTCGTCGGCAGCGACTGGCTACGGTGCGG CGATCTCGGAGCTGACAAACATGCGGTGGTGGTCGGGGCCGGCATCGGATTCGATGGTGGCC GCCGTCCTGCCCTTTGTCGGCTGGCTGAGTACCACCGCGACGCTAGCCGAACAGGCCGCGATG

CAGGCTAGGGCGGCCGCAGCGGCCTTTGAAGCCGCCTTCGCCATGACGGTGCCCCCGCCGGC GATCGCGGCCAACCGGACCTTGTTGATGACGCTCGTCGATACCAACTGGTTCGGGCAAAACAC GCCGGCGATCGCCACCACCGAGTCCCAATACGCCGAGATGTGGGCCCAAGACGCCGCCGCGA TGTACGGCTATGCCAGCGCCGCGGCACCCGCCACGGTTTTGACTCCGTTCGCACCACCGCCGC AAACCACCAACGCGACCGGCCTCGTCGGCCACGCAACAGCGGTGGCCGCGCTGCGGGGGCAG CACAGCTGGGCCGCGGCGATTCCATGGAGCGACATACAGAAATACTGGATGATGTTCCTGGGC GCCCTCGCCACTGCCGAAGGGTTCATTTACGACAGCGGTGGGTTAACGCTGAATGCTCTGCAGT

TCGTCGGCGGGATGTTGTGGAGCACCGCATTGGCAGAAGCCGGTGCGGCCGAGGCAGCGGCC GGCGCGGGTGGAGCCGCTGGATGGTCGGCGTGGTCGCAGCTGGGAGCTGGACCGGTGGCGG CGAGCGCGACTCTGGCCGCCAAGATCGGACCGATGTCGGTGCCGCCGGGCTGGTCCGCACCG CCCGCCACGCCCCAGGCGCAAACCGTCGCGCGATCGATTCCCGGTATTCGCAGCGCCGCCGA GGCGGCTGAAACATCGGTCCTACTCCGGGGGGCACCGACTCCGGGCAGGAGTCGCGCCGCCC

ATATGGGACGCCGATATGGAAGACGACTCACCGTGATGGCTGACCGGCCGAACGTCGGATAG

>Rv1745c - similar to Q46822 ORF_0182 TB.seq 1971381 :1971989 MW:22490 >emb|AL123456|MTBH37RV:c1971989-1971378, Rv1745c SEQ ID NO:60 ATGACCCGCAGCTACCGGCCAGCTCCACCGATCGAGCGGGTGGTTTTGCTCAACGACCGCGGC

GACGCGACAGGTGTGGCCGACAAGGCCACCGTGCACACCGGCGACACCCCTTTGCACCTCGC GTTCTCCAGCTATGTGTTCGATCTGCACGATCAGCTGTTGATCACGCGGCGGGCCGCCACCAAG AGGACGTGGCCGGCGGTATGGACCAACAGTTGCTGCGGGCACCCCCTGCCTGGCGAATCGCT ACCCGGCGCCATACGCCGGCGGCTCGCTGCCGAACTCGGACTGACCCCAGATCGGGTCGATC TGATCCTGCCGGGGTTCCGCTACCGGGCCGCTATGGCCGATGGCACCGTGGAAAACGAGATCT

GCCCCGTCTACCGAGTCCAGGTTGACCAACAGCCCCGGCCGAACTCGGACGAGGTCGACGCG ATCCGCTGGTTGTCCTGGGAACAATTCGTGCGCGATGTTACCGCCGGCGTAATCGCCCCGGTAT CCCCTTGGTGCCGCTCACAACTGGGCTACCTGACCAAACTTGGACCATGTCCGGCACAGTGGC CCGTGGCCGACGACTGCCGGCTACCGAAAGCCGCACATGGTAATTAA

>Rv1800 - TB.seq 2039451 :2041415 MW:67068 >emb|AL123456|MTBH37RV:2039451-2041418, PPE SEQ ID NO:61

ATGCTGCCGAATTTCGCGGTGCTGCCCCCCGAGGTCAATTCGGCGAGGGTGTTCGCCGGTGCG GGGTCGGCGCCGATGTTAGCGGCAGCGGCCGCCTGGGATGATCTAGCCTCCGAGCTGCATTGT GCTGCAATGTCATTCGGGTCGGTTACGTCGGGATTGGTGGTTGGGTGGTGGCAGGGATCGGCG

TCGGCGGCGATGGTGGACGCAGCCGCGTCGTACATCGGGTGGCTGAGCACGTCGGCTGCCCA CGCCGAGGGCGCGGCCGGTCTGGCTCGGGCCGCGGTATCGGTGTTCGAGGAGGCGCTGGCC GCGACGGTGCATCCGGCGATGGTTGCGGCAAATCGCGCCCAGGTGGCGTCGCTGGTAGCGTC GAACTTGTTTGGGCAGAACGCGCCTGCGATCGCCGCGCTCGAATCCTTGTATGAGTGTATGTGG GCCCAGGATGCAGCGGCCATGGCGGGTTATTACGTTGGGGCTTCGGCGGTGGCCACACAGTTG

GCATCGTGGCTGCAACGGCTACAGAGCATCCCCGGCGCCGCCAGTCTTGATGCCCGTCTGCCG AGCTCGGCCGAGGCACCGATGGGAGTCGTCCGCGCGGTCAACAGCGCGATCGCCGCCAATGC GGCTGCGGCACAAACCGTTGGCCTGGTCATGGGAGGCAGCGGCACGCCAATACCGTCGGCCA GATATGTCGAGCTCGCGAACGCGCTGTACATGAGTGGCAGCGTCCCGGGTGTTATCGCGCAGG CGCTCTTCACGCCCCAAGGGCTCTACCCGGTGGTCGTGATCAAGAACCTCACTTTCGATTCCTC GGTGGCGCAGGGTGCCGTCATTCTCGAAAGTGCGATTCGGCAGCAAATTGCCGCCGGCAACAA CGTCACCGTCTTCGGCTACTCGCAGAGCGCCACGATCTCGTCACTAGTGATGGCCAATCTTGCG

GCTTCGGCCGACCCGCCGTCTCCAGACGAGCTTTCCTTCACGCTGATCGGCAATCCCAACAACC CCAATGGCGGGGTTGCCACCAGGTTCCCGGGGATCTCCTTTCCAAGCTTGGGCGTGACGGCCA CCGGGGCCACTCCGCACAATCTGTACCCGACCAAGATCTACACCATCGAATACGACGGCGTCG CCGACTTTCCGCGGTACCCGCTCAACTTTGTGTCGACCCTCAACGCCATTGCCGGCACCTACTA CGTGCACTCCAACTACTTCATCCTGACGCCGGAACAAATTGACGCAGCGGTTCCGCTGACCAAT

ACGGTCGGTCCCACGATGACCCAGTACTACATCATTCGCACGGAGAACCTGCCGCTGCTAGAG CCACTGCGATCGGTGCCGATCGTGGGGAACCCACTGGCGAACCTGGTTCAACCAAACTTGAAG GTGATTGTTAACCTGGGCTACGGCGACCCGGCCTATGGTTATTCGACCTCGCCGCCCAATGTTG CGACTCCGTTCGGGTTGTTCCCAGAGGTCAGCCCGGTCGTCATCGCCGACGCTCTCGTCGCCG GGACCCAGCAGGGAATCGGCGATTTCGCCTACGACGTCAGCCACCTCGAACTGCCGTTGCCGG

CAGACGGGTCGACGATGCCAAGCACCGCACCGGGCTCGGGTACGCCGGTCCCCCCGCTCTCG ATCGACAGCCTGATAGACGACCTGCAGGTGGCTAACCGCAACCTCGCCAACACGATTTCGAAG GTGGCCGCGACGAGCTACGCGACGGTGCTCCCAACCGCCGACATCGCCAATGCGGCGTTGAC GATCGTGCCGTCGTACAACATCCACCTTTTTTTGGAGGGCATCCAGCAAGCGCTCAAGGGCGAC CCGATGGGACTCGTCAACGCGGTCGGATACCCACTCGCGGCCGACGTGGCACTGTTCACGGCC

GCAGGCGGTCTTCAGCTCTTGATCATCATCAGCGCGGGCCGAACGATTGCCAATGACATCTCGG CCATTGTCCCCTGA

>Rv1844c gnd 6-phosphogluconate dehydrogenase (Gram -) TB.seq 2093732:2095186 MW:51548 >emb|AL123456|MTBH37RV:c2095186-2093729, gnd SEQ ID NO:62

ATGAGTTCGTCGGAATCGCCAGCCGGCATCGCGCAGATCGGCGTCACTGGCCTGGCCGTGATG GGTTCCAACATCGCCCGAAACTTCGCCCGGCACGGCTACACCGTGGCAGTGCACAATCGGTCG GTCGCCAAGACCGACGCGCTGCTTAAGGAGCACAGCTCAGACGGCAAGTTCGTGCGCAGTGAA ACGATCCCCGAATTTCTTGCCGCACTGGAAAAACCGCGTCGGGTGCTGATCATGGTCAAGGCC GGAGAGGCCACTGACGCTGACGCTGTCATCAACGAACTTGCTGACGCCATGGAACCCGGCGAC

ATCATCATCGACGGCGGCAATGCGTTGTACACCGACACCATGCGCCGCGAGAAAGCGATGCGT GAGCGGGGCTTGCACTTCGTCGGGGCCGGGATCTCCGGCGGCGAAGAGGGCGCGTTGAACGG GCCGTCGATCATGCCCGGCGGACCCGCCGAGTCATACCAATCGCTGGGTCCGCTGCTCGAGGA GATCTCCGCGCATGTCGACGGCGTGCCGTGCTGCACCCACATTGGCCCGGACGGCTCCGGGC ACTTCGTCAAGATGGTCCACAACGGCATCGAGTACTCCGACATGCAGCTCATCGGTGAGGCCTA

CCAGCTGATGCGCGACGGGCTAGGTCTGACCGCGCCGGCGATCGCCGATGTGTTCACCGAGT GGAACAATGGCGATCTGGACAGCTACCTGGTCGAGATCACCGCCGAGGTGCTGCGGCAGACCG ATGCCAAGACCGGCAAACCGCTCGTCGACGTCATCGTGGACCGGGCCGAGCAGAAAGGCACC GGCCGTTGGACCGTCAAGTCCGCGCTGGACCTGGGTGTGCCGGTGACCGGCATCGCCGAAGC GGTGTTTGCCCGCGCTCTCTCGGGATCCGTGGGGCAACGCTCGGCCGCCAGCGGTCTGGCTTC GGGCAAGCTCGGCGAGCAGCCCGCCGACCCCGCCACGTTCACCGAAGACGTCCGCCAGGCGT TGTACGCCTCCAAGATCGTGGCCTACGCTCAGGGCTTCAACCAGATCCAGGCCGGCAGCGCCG

AATTCGGCTGGGACATCACGCCGGGCGACCTGGCCACCATCTGGCGTGGCGGCTGCATCATCC GGGCGAAGTTCCTCAACCACATCAAGGAAGCCTTTGACGCCAGCCCGAACCTGGCCAGTCTGA TTGTGGCCCCGTATTTCCGCGGCGCCGTCGAATCGGCGATCGACAGTTGGCGGCGTGTGGTGT CGACGGCGGCCCAACTGGGTATCCCGACCCCGGGATTCTCGTCGGCCCTGTCGTATTACGACG CGCTGCGCACCGCGCGGCTGCCCGCTGCACTCACCCAGGCCCAGCGCGACTTCTTCGGCGCA

CACACCTACGGCCGGATCGACGAACCAGGCAAGTTCCACACACTATGGAGTTCAGACCGCACC GAAGTACCGGTGTAG

>Rv1900c lipJ TB.seq 2146246:2147631 MW:49685 >emb|AL123456|MTBH37RV:c2147631-2146243, lipJ SEQ ID NO:63

GTGGCGCAGGCTCCCCACATTCACAGGACCCGCTACGCAAAATGCGGCGACATGGATATCGCC TACCAGGTGCTGGGTGACGGTCCGACGGATCTGCTGGTGTTGCCGGGGCCGTTCGTGCCGATC GACTCGATCGACGACGAGCCATCGCTGTACCGTTTCCATCGCCGTCTTGCGTCATTCAGCAGGG TGATCCGCCTCGACCATCGTGGGGTCGGCCTGTCGTCACGGCTCGCCGCGATAACCACGCTGG GGCCGAAGTTCTGGGCCCAGGACGCGATCGCGGTGATGGACGCGGTCGGATGCGAGCAGGCG

ACAATTTTCGCGCCCAGTTTCCACGCCATGAACGGACTTGTTCTCGCCGCCGACTACCCCGAGC GGGTGCGCAGCCTGATCGTCGTCAACGGCTCGGCGCGCCCACTATGGGCGCCCGACTACCCG GTAGGCGCCCAGGTTCGTCGAGCTGACCCGTTCCTGACGGTGGCGCTGGAACCGGATGCCGTC GAGCGGGGCTTCGACGTGCTGAGCATCGTGGCTCCTACCGTGGCCGGAGATGACGTGTTTCGA GCCTGGTGGGATCTCGCCGGCAACCGTGCCGGACCGCCGAGCATTGCCCGTGCCGTTTCAAAG

GTCATAGCCGAGGCCGACGTACGAGATGTCTTGGGACACATCGAGGCTCCAACACTGATCTTGC ACCGTGTCGGATCGACGTACATCCCGGTGGGACATGGTCGCTACCTCGCCGAGCACATCGCTG GATCCCGCTTGGTCGAACTACCCGGCACCGATACCCTGTACTGGGTTGGCGACACCGGGCCGA TGCTCGATGAAATCGAGGAATTCATCACCGGCGTGCGCGGCGGCGCTGACGCCGAGCGCATGC TTGCCACCATCATGTTTACCGACATCGTCGGCTCGACCCAGCACGCCGCCGCGCTCGGCGACG

ACCGATGGCGCGACCTGTTGGACAACCACGACACCATCGTGTGCCACGAAATCCAGCGGTTCG GCGGTCGCGAAGTGAACACGGCCGGTGACGGTTTCGTCGCGACGTTCACCAGTCCGAGTGCC GCGATCGCGTGCGCGGACGACATCGTCGACGCGGTCGCCGCGCTGGGTATTGAGGTCCGGAT CGGTATTCATGCGGGCGAGGTCGAGGTGCGCGATGCCTCGCACGGTACCGACGTCGCCGGCG TGGCCGTGCATATCGGTGCGCGCGTCTGCGCGCTGGCCGGACCCAGTGAGGTGCTGGTGTCC

TCGACCGTGCGAGACATCGTCGCCGGATCACGGCACCGGTTCGCCGAGCGTGGTGAGCAGGA ACTCAAGGGCGTACCGGGCAGATGGCGGCTATGCGTGCTCATGCGCGACGACGCCACCCGCA CGCGCTAA

>Rv1967 - TB.seq 2210599:2211624 MW:36516 >emb|AL123456|MTBH37RV:2210599-2211627, Rv1967 SEQ ID NO:64

ATGAGGGAGAACCTGGGGGGCGTCGTGGTGCGCCTCGGCGTCTTCCTGGCGGTATGCCTGCT GACGGCGTTCCTGCTGATTGCCGTCTTCGGGGAGGTGCGCTTCGGCGACGGCAAGACCTACTA CGCCGAGTTCGCCAACGTGTCCAATCTGCGAACGGGCAAGCTGGTGCGCATCGCCGGCGTCGA GGTCGGCAAGGTCACCAGGATCTCCATCAACCCCGACGCGACGGTGCGGGTGCAGTTCACCGC CGACAACTCGGTCACCCTCACGCGGGGCACCCGGGCGGTGATCCGCTACGACAACCTGTTCGG

TGACCGCTATTTGGCGCTGGAGGAAGGGGCCGGCGGACTCGCCGTTCTTCGTCCCGGTCACAC GATTCCGTTGGCGCGCACCCAACCGGCGTTGGATCTGGATGCCCTGATCGGTGGATTCAAGCC GCTGTTTCGTGCGCTGAACCCCGAGCAGGTCAACGCGCTGAGCGAACAGTTGCTGCACGCGTT TGCCGGACAGGGGCCCACGATCGGGTCATTGCTGGCCCAGTCCGCGGCCGTGACCAACACCC TGGCCGACCGTGATCGGCTGATCGGGCAGGTGATCACCAACCTCAACGTGGTGCTGGGCTCGC

TGGGCGCTCACACCGATCGGTTGGACCAGGCGGTGACGTCGCTATCAGCGTTGATTCACCGGC TCGCGCAACGCAAGACCGACATCTCCAACGCCGTGGCCTACACCAACGCCGCCGCCGGCTCG GTCGCCGATCTGCTGTCGCAGGCTCGCGCGCCGTTGGCGAAGGTGGTTCGCGAGACCGATCG GGTGGCCGGCATCGCGGCCGCCGACCACGACTACCTCGACAATCTGCTCAACACGCTGCCGGA CAAATACCAGGCGCTGGTCCGCCAGGGTATGTACGGCGACTTCTTCGCCTTCTACCTGTGCGAC

GTCGTGCTCAAGGTCAACGGCAAGGGCGGCCAGCCGGTGTACATCAAGCTGGCCGGTCAGGA CAGCGGGCGGTGCGCGCCGAAATGA

>Rv1975 - TB.seq 2218050:2218712 MW:23650 >emb|AL123456|MTBH37RV:2218050-2218715, Rv1975 SEQ ID NO:65

ATGTCGCGTCGAGCATCGGCCACGTGTGCCTTGTCCGCGACCACCGCCGTCGCCATAATGGCT GCTCCCGCCGCACGGGCCGACGACAAGCGGCTCAACGACGGCGTGGTCGCCAACGTCTACAC CGTTCAACGTCAGGCCGGCTGCACCAACGACGTCACGATCAACCCGCAACTACAATTGGCCGC CCAATGGCACACCCTCGATCTGCTGAACAACCGGCACCTCAACGACGACACCGGTTCTGACGG ATCCACACCGCAAGACCGCGCGCATGCCGCCGGCTTCCGCGGGAAAGTCGCTGAAACCGTGG

CGATCAATCCCGCCGTAGCGATCAGCGGCATCGAGTTGATAAACCAGTGGTACTACAACCCCGC GTTTTTCGCGATCATGTCCGACTGCGCCAACACCCAGATCGGGGTGTGGTCAGAAAACAGCCC GGATCGCACCGTCGTGGTGGCCGTTTACGGACAGCCCGATCGACCTTCCGCGATGCCGCCCAG GGGAGCGGTAACCGGACCGCCGTCCCCGGTGGCCGCGCAAGAGAACGTTCCTATCGACCCCA GCCCCGACTACGACGCCAGCGACGAGATCGAATACGGCATCAACTGGCTGCCATGGATCCTGC

GCGGCGTGTACCCGCCGCCCGCAATGCCGCCGCAGTAG >Rv1981c nrdF ribonucleotide reductase small subunit TB.seq 2224221 :2225186 MW:36591 >emb|AL123456|MTBH37RV:c2225186-2224218, nrdF SEQ ID NO:66

ATGACCGGCAAGCTCGTTGAGCGGGTGCACGCAATCAATTGGAACCGGTTGCTCGATGCTAAA GATTTGCAGGTCTGGGAACGTTTGACCGGTAACTTTTGGTTGCCGGAAAAGATTCCGCTCTCCA ACGACCTGGCATCTTGGCAAACGTTGAGTTCCACCGAGCAGCAGACGACGATCCGGGTGTTCA

CCGGCTTGACCCTGCTCGACACCGCGCAGGCGACGGTGGGAGCAGTGGCCATGATCGACGAC GCGGTCACCCCCCACGAAGAGGCGGTCCTGACCAACATGGCGTTCATGGAGTCAGTGCACGCC AAGAGCTACAGCTCGATCTTCTCGACCCTGTGCTCGACCAAGCAGATCGACGATGCCTTCGACT GGTCGGAACAGAACCCTTACCTGCAGCGAAAAGCGCAGATCATCGTCGACTACTACCGCGGTG ACGACGCGCTCAAGCGCAAAGCATCGTCGGTAATGCTGGAGTCCTTCCTGTTCTACTCCGGCTT

CTACCTGCCCATGTACTGGTCGTCGCGGGGTAAGCTCACCAACACCGCCGATCTGATCCGGCT GATCATCCGAGATGAAGCCGTCCACGGCTACTACATCGGCTACAAATGTCAACGAGGTTTGGCC GACCTGACCGACGCCGAGCGGGCCGACCACCGCGAATACACCTGCGAGCTGCTGCACACGCT CTACGCGAACGAGATCGACTATGCGCACGACTTGTACGACGAGTTGGGCTGGACCGACGACGT TTTGCCCTACATGCGTTACAACGCCAACAAGGCGCTAGCCAACCTGGGATACCAGCCTGCATTC

GATCGTGACACCTGCCAGGTGAACCCGGCCGTGCGCGCAGCTCTCGACCCCGGTGCAGGGGA GAACCACGACTTTTTCTCCGGCTCCGGAAGCTCATACGTAATGGGCACCCACCAACCCACCACC GACACCGACTGGGACTTCTAA

>Rv2092c helY helicase, Ski2 subfamily TB.seq 2349335:2352052 MW:99576

>emb|AL123456|MTBH37RV:c2352052-2349332, helY SEQ ID NO:67

GTGACTGAGCTGGCCGAGCTGGACCGGTTCACCGCGGAACTACCGTTCTCGCTCGACGACTTT CAGCAGCGGGCTTGCAGCGCGCTGGAACGCGGCCACGGTGTGCTGGTGTGCGCGCCGACCG GCGCTGGCAAGACGGTGGTCGGCGAGTTCGCCGTGCACCTGGCGCTGGCGGCCGGCAGTAAA TGTTTCTACACCACGCCGCTGAAAGCCCTGAGCAACCAAAAGCACACCGATCTCACAGCACGCT

ACGGCCGTGACCAGATCGGGCTGCTGACCGGTGACCTGTCGGTCAACGGCAACGCGCCGGTG GTGGTGATGACCACCGAAGTGCTGCGCAACATGCTCTACGCGGATTCGCCTGCGCTGCAGGGG CTTTCCTATGTGGTGATGGATGAGGTGCATTTCCTCGCCGACCGGATGCGGGGTCCGGTGTGG GAGGAGGTGATCCTGCAACTGCCCGACGACGTGCGGGTGGTCAGCCTGTCGGCGACGGTGAG CAACGCCGAGGAGTTCGGCGGTTGGATCCAGACGGTGCGGGGCGACACCACGGTGGTGGTCG

ACGAGCATCGGCCGGTGCCGTTGTGGCAACACGTCTTGGTGGGCAAGCGCATGTTCGACCTGT TCGATTACCGGATCGGCGAAGCCGAAGGGCAGCCCCAAGTCAACCGCGAGTTGCTGCGCCACA TCGCGCATCGCCGTGAGGCCGACCGGATGGCCGATTGGCAGCCTCGGCGCCGAGGCTCGGGC CGGCCCGGCTTCTACCGGCCACCCGGCCGACCCGAGGTGATCGCCAAACTCGACGCTGAAGG GCTGTTGCCGGCGATCACCTTCGTGTTCTCCCGGGCCGGTTGTGACGCCGCGGTCACCCAATG

CCTGCGGTCACCGCTGCGGTTGACCAGCGAAGAGGAGCGCGCACGGATCGCCGAGGTGATCG ACCACCGCTGCGGTGACCTGGCCGACTCCGACCTGGCGGTACTCGGCTACTACGAATGGCGG GAAGGGTTACTGCGCGGTCTGGCCGCCCACCACGCGGGCATGTTGCCGGCCTTCCGGCACAC GGTGGAGGAGCTGTTCACCGCCGGTTTGGTCAAGGCTGTATTCGCCACCGAGACTCTGGCGCT CGGTATCAACATGCCGGCCCGCACGGTGGTGCTGGAGCGGCTGGTGAAGTTCAACGGTGAGCA GCACATGCCGCTGACGCCGGGGGAGTACACCCAACTGACCGGTCGCGCCGGCCGGCGCGGTA TCGACGTCGAGGGTCACGCGGTGGTGATCTGGCACCCGGAAATTGAACCGTCCGAGGTGGCG

GGCCTGGCCTCCACCCGCACCTTTCCGCTGCGCAGCTCGTTTGCCCCGTCGTACAACATGACG ATCAACCTGGTGCACCGGATGGGTCCGCAACAGGCGCACCGACTGCTCGAGCAGTCGTTCGCC CAATATCAGGCCGACCGATCCGTGGTCGGACTGGTCCGCGGAATTGAGCGGGGCAACAGGATA CTCGGCGAGATCGCAGCCGAACTGGGCGGATCTGATGCGCCCATCCTCGAATACGCTCGATTG CGCGCGCGGGTGTCCGAGCTGGAACGTGCGCAGGCCCGCGCGTCGCGGTTACAGCGACGGC

AGGCGGCCACCGATGCGCTGGCCGCGCTGCGCCGCGGTGACATCATCACCATCACCCACGGC CGCCGCGGTGGTCTGGCCGTCGTCCTGGAATCAGCCCGCGACCGCGACGACCCGCGTCCGCT GGTGCTAACCGAACACCGATGGGCGGGACGGATCTCCTCGGCCGACTACTCGGGCACGACGC CGGTGGGGTCGATGACGCTGCCCAAGCGGGTGGAGCACCGCCAGCCGCGGGTCCGGCGTGA CCTGGCCTCGGCGCTGCGATCGGCAGCCGCGGGTCTGGTTATTCCAGCCGCCCGGCGCGTCA

GCGAGGCCGGCGGGTTTCACGATCCGGAGCTGGAGTCGTCGCGCGAACAATTGCGCCGTCAT CCGGTGCATACCTCGCCCGGGCTCGAGGACCAGATCCGCCAGGCCGAGCGTTACTTACGCATC GAACGCGACAACGCGCAATTAGAGAGGAAGGTCGCCGCCGCCACCAACTCGTTGGCCCGCAC GTTCGACCGATTCGTCGGGCTGCTCACCGAACGGGAGTTCATCGATGGCCCGGCCACTGATCC CGTGGTCACCGACGACGGCCGGCTGCTGGCGCGGATTTACAGCGAGAGCGACCTGTTGGTGG

CCGAGTGCCTACGTACAGGTGCGTGGGAGGGTTTAAAGCCGGCCGAATTGGCGGGGGTGGTG TCGGCGGTGGTCTACGAGACGCGCGGTGGTGACGGCCAGGGCGCCCCGTTCGGAGCCGATGT GCCCACACCGCGGTTACGGCAGGCTCTGACTCAGACATCAAGGCTGTCCACGACATTGCGCGC CGACGAGCAGGCACACCGCATCACCCCGAGTCGCGAACCCGACGATGGCTTTGTCAGAGTCAT CTACCGCTGGTCGCGAACCGGTGATCTAGCGGCGGCATTGGCCGCTGCCGACGTGAACGGCA

GCGGATCACCGTTATTGGCAGGGGATTTCGTGCGTTGGTGCCGTCAGGTGCTCGATCTGCTGG ACCAAGTTCGTAACGCTGCGCCCAACCCCGAACTGCGGGCTACCGCAAAGCGCGCTATCGGTG ACATTCGGCGCGGCGTCGTCGCGGTTGACGCCGGGTAG

>Rv2101 helZ helicase, Snf2/Rad54 family TB.seq 2360238:2363276 MW:111632

>emb|AL123456|MTBH37RV:2360238-2363279, helZ SEQ ID NO:68

ATGCTGGTTTTGCACGGCTTCTGGTCCAACTCCGGCGGGATGCGGCTGTGGGCGGAGGACTCC GATCTGCTGGTGAAGAGCCCGAGTCAGGCGCTGCGCTCCGCGCGGCCACACCCGTTCGCGGC GCCCGCTGACCTGATCGCCGGCATACATCCGGGCAAACCCGCAACCGCCGTTTTGCTGTTGCC GTCGTTGCGATCGGCGCCGCTGGACTCGCCGGAGCTGATCCGGCTCGCCCCGCGCCCGGCCG

CGCGAACCGATCCGATGCTGTTGGCGTGGACGGTACCGGTGGTGGACCTGGACCCCACCGCG GCGTTGGCCGCCTTCGACCAGCCCGCCCCCGACGTCCGCTACGGCGCGTCCGTCGACTACCT GGCCGAGCTGGCCGTTTTCGCGCGCGAGTTGGTCGAGCGTGGTCGCGTGCTGCCCCAGCTGC GCCGCGACACCCACGGCGCGGCCGCCTGCTGGCGTCCGGTGTTGCAGGGACGCGACGTGGTC GCGATGACCTCGCTGGTCTCGGCGATGCCGCCGGTCTGCCGCGCCGAAGTTGGTGGGCACGA CCCGCACGAACTGGCAACCTCGGCTCTGGACGCGATGGTCGACGCCGCCGTGCGCGCGGCGC TGTCACCGATGGACCTGCTGCCCCCGCGACGGGGTCGCTCCAAACGGCATCGGGCCGTGGAG

GCTTGGCTGACCGCGTTGACCTGCCCGGACGGCCGGTTCGACGCGGAGCCCGACGAACTCGA CGCGCTGGCCGAGGCGTTGCGGCCATGGGACGACGTCGGTATCGGCACCGTCGGCCCGGCGC GGGCGACGTTTCGGCTGTCCGAAGTCGAGACCGAAAACGAGGAGACGCCCGCGGGCTCGTTG TGGAGGCTGGAGTTCTTATTGCAGTCGACGCAGGACCCCAGCCTGCTGGTCCCCGCCGAGCAG GCATGGAACGACGACGGCAGCCTGCGCCGCTGGCTGGACCGGCCGCAGGAGCTGCTGCTGAC

CGAACTGGGCCGGGCCTCTCGGATTTTCCCCGAGCTCGTCCCGGCGCTGCGCACCGCGTGCC CGTCCGGGCTTGAGCTCGACGCCGACGGCGCCTACCGATTCCTGTCGGGTACGGCCGCGGTG CTCGACGAGGCTGGGTTTGGCGTGCTGCTGCCGTCCTGGTGGGACCGCCGCCGCAAGCTGGG CTTGGTCCTGTCCGCATATACCCCGGTCGACGGCGTGGTGGGCAAGGCCAGCAAGTTCGGCCG CGAGCAGCTCGTCGAGTTCCGCTGGGAGCTGGCCGTGGGCGACGATCCGCTCAGCGAGGAGG

AGATCGCGGCGCTGACCGAAACCAAGTCCCCGCTGATCCGGCTGCGTGGCCAGTGGGTCGCG CTCGATACCGAACAGATGCGCCGCGGGCTGGAGTTTTTGGAGCGTAAGCCAACCGGCCGCAAG ACCACCGCCGAGATCCTCGCGCTGGCCGCCAGCCACCCCGACGACGTGGACACCCCGCTCGA GGTCACCGCCGTACGCGCCGACGGCTGGCTCGGGGACCTGCTCGCCGGGGCCGCCGCGGCG TCGCTGCAGCCGTTGGACCCGCCCGACGGATTCACCGCGACGCTGCGTCCCTACCAGCAGCGC

GGTCTGGCGTGGCTGGCGTTTTTGTCCTCGCTCGGTTTGGGCAGCTGCCTGGCCGACGACATG GGCCTGGGCAAGACGGTGCAGCTATTGGCCCTGGAAACCTTGGAATCCGTTCAGCGCCACCAG GATCGCGGCGTCGGACCCACACTGCTACTGTGCCCGATGTCGTTGGTGGGCAACTGGCCGCAG GAAGCGGCCAGGTTTGCACCCAACCTGCGGGTGTACGCCCACCACGGGGGCGCCCGGCTGCA CGGCGAGGCGTTGCGCGACCACCTCGAGCGCACCGACCTGGTCGTGAGCACCTATACCACCG

CCACCCGCGACATCGACGAGCTGGCGGAATACGAATGGAACCGGGTGGTGCTGGACGAGGCC CAGGCGGTGAAGAACAGCCTGTCCCGGGCGGCCAAGGCGGTGCGACGGCTACGCGCGGCGC ACCGGGTCGCGCTGACCGGGACACCGATGGAGAACCGGCTCGCCGAGCTGTGGTCGATCATG GACTTCCTCAACCCGGGCCTGCTCGGATCCTCCGAACGCTTCCGCACCCGCTACGCGATCCCG ATCGAGCGGCACGGGCACACCGAACCGGCCGAACGGCTGCGCGCATCGACGCGGCCCTACAT

CCTGCGCCGGCTCAAGACCGACCCGGCGATCATCGACGATCTGCCGGAGAAGATCGAGATCAA GCAGTACTGCCAACTCACCACCGAGCAGGCGTCGCTGTATCAGGCCGTCGTCGCCGACATGAT GGAAAAGATCGAAAACACCGAAGGGATCGAGCGGCGCGGCAACGTGCTGGCCGCGATGGCCA AGCTCAAACAGGTGTGCAACCACCCCGCCCAGCTGCTGCACGATCGCTCCCCGGTCGGTCGGC GGTCCGGGAAGGTGATCCGGCTCGAGGAGATCCTGGAAGAGATCCTGGCCGAGGGCGACCGG

GTGCTGTGTTTTACCCAGTTCACCGAGTTCGCCGAGCTGCTGGTGCCGCACCTGGCCGCACGC TTCGGCCGTGCCGCCCGAGACATTGCCTACCTGCACGGTGGCACCCCGAGGAAGCGGCGTGA CGAGATGGTGGCCCGGTTCCAGTCCGGTGACGGCCCGCCCATTTTTCTGCTGTCGTTGAAGGC GGGCGGTACCGGGCTGAACCTCACCGCCGCCAATCATGTTGTGCACCTGGACCGCTGGTGGAA CCCGGCGGTCGAGAACCAGGCGACGGACCGGGCGTTTCGGATCGGGCAGCGGCGCACGGTG CAGGTCCGCAAGTTCATCTGCACCGGCACCCTCGAGGAGAAGATCGACGAAATGATCGAGGAG AAAAAGGCGCTGGCCGACTTGGTGGTCACCGACGGCGAAGGCTGGCTGACCGAACTGTCCACC

CGCGATCTGCGCGAGGTGTTCGCGCTGTCCGAAGGCGCCGTCGGTGAGTAG

>Rv2110c prcB proteasome [beta]-type subunit 2 TB.seq 2369727:2370599 MW:30274 >emb|AL123456|MTBH37RV:c2370599-2369724, prcB SEQ ID NO:69 GTGACCTGGCCGTTGCCCGATCGCCTGTCCATTAATTCACTCTCTGGAACACCCGCTGTAGACC

TATCTTCTTTCACTGACTTCCTGCGCCGCCAGGCGCCGGAGTTGCTGCCGGCAAGCATCAGCG GCGGTGCGCCACTCGCAGGCGGCGATGCGCAACTGCCGCACGGCACCACCATTGTCGCGCTG AAATACCCCGGCGGTGTTGTCATGGCGGGTGACCGGCGTTCGACGCAGGGCAACATGATTTCT GGGCGTGATGTGCGCAAGGTGTATATCACCGATGACTACACCGCTACCGGCATCGCTGGCACG GCTGCGGTCGCGGTTGAGTTTGCCCGGCTGTATGCCGTGGAACTTGAGCACTACGAGAAGCTC

GAGGGTGTGCCGCTGACGTTTGCCGGCAAAATCAACCGGCTGGCGATTATGGTGCGTGGCAAT CTGGCGGCCGCGATGCAGGGTCTGCTGGCGTTGCCGTTGCTGGCGGGCTACGACATTCATGCG TCTGACCCGCAGAGCGCGGGTCGTATCGTTTCGTTCGACGCCGCCGGCGGTTGGAACATCGAG GAAGAGGGCTATCAGGCGGTGGGCTCGGGTTCGCTGTTCGCGAAGTCGTCGATGAAGAAGTTG TATTCGCAGGTTACCGACGGTGATTCGGGGCTGCGGGTGGCGGTCGAGGCGCTCTACGACGCC

GCCGACGACGACTCCGCCACCGGCGGTCCGGACCTGGTGCGGGGCATCTTTCCGACGGCGGT GATCATCGACGCCGACGGGGCGGTTGACGTGCCGGAGAGCCGGATTGCCGAATTGGCCCGCG CGATCATCGAAAGCCGTTCGGGTGCGGATACTTTCGGCTCCGATGGCGGTGAGAAGTGA

>Rv2118c - = B2126_C1_165 (83.6%) TB.seq 2377471 :2378310 MW:30091

>emb|AL123456|MTBH37RV:c2378310-2377468, Rv2118c SEQ ID NO:70

GTGTCAGCAACCGGCCCATTCAGCATCGGCGAACGTGTTCAGCTCACCGACGCTAAGGGGCGC CGCTACACCATGTCGCTGACTCCCGGTGCCGAATTCCACACTCATCGTGGCTCGATCGCCCACG ACGCGGTGATCGGGTTGGAGCAAGGCAGCGTGGTCAAATCCAGCAACGGCGCCCTGTTCCTGG TGCTGCGCCCGCTGCTGGTCGACTACGTCATGTCGATGCCGCGCGGCCCGCAGGTGATCTATC

CCAAAGATGCGGCCCAGATCGTGCATGAGGGCGACATATTTCCCGGCGCGCGGGTGCTGGAG GCAGGAGCCGGATCCGGTGCTCTGACCTTGTCTTTGCTGCGGGCGGTTGGGCCGGCCGGACA GGTGATCTCCTACGAACAGCGCGCCGATCATGCCGAACACGCCCGGCGCAATGTGAGCGGCTG CTACGGCCAGCCGCCGGACAACTGGCGACTGGTCGTCAGCGACCTCGCCGACTCCGAACTGC CCGACGGATCCGTTGATCGGGCCGTGCTCGACATGCTGGCGCCGTGGGAGGTGCTCGACGCG

GTATCGCGGCTGCTGGTCGCCGGCGGAGTGCTGATGGTCTACGTGGCCACCGTCACTCAGCTG TCGAGGATCGTGGAGGCACTGCGGGCCAAGCAGTGCTGGACCGAACCGAGAGCCTGGGAGAC GCTGCAGCGGGGCTGGAACGTCGTAGGGTTGGCGGTTCGGCCGCAGCATTCGATGCGCGGGC ATACCGCGTTCCTGGTAGCAACGCGCCGGTTGGCGCCGGGGGCTGTGGCTCCGGCGCCGCTA GGTCGTAAGCGCGAGGGACGCGACGGGTAG

>Rv2144c - TB.seq 2404166:2404519 MW.12028

>emb|AL123456|MTBH37RV:c2404519-2404163, Rv2144c SEQ ID NO:71

ATGCTGATCATTGCGCTGGTCTTGGCCCTGATTGGGCTCCTGGCCTTGGTGTTCGCGGTGGTCA CCAGCAACCAGCTAGTGGCCTGGGTATGCATCGGGGCCAGCGTGCTGGGTGTGGCGTTGCTGA TCGTCGATGCGTTGCGAGAACGCCAGCAAGGTGGCGCGGACGAAGCTGATGGGGCTGGGGAA ACGGGTGTCGCGGAGGAAGCCGACGTCGACTACCCGGAGGAAGCCCCCGAGGAGAGCCAAGC

CGTCGACGCCGGTGTCATCGGCAGTGAGGAGCCATCGGAGGAGGCCAGCGAAGCGACCGAGG AGTCGGCGGTATCGGCGGACCGAAGCGACGACAGCGCCAAGTAG

>Rv2146c - TB.seq 2405667:2405954 MW:10805 >emb|AL123456|MTBH37RV:c2405954-2405664, Rv2146c SEQ ID NO:72

TTGGTGGTGTTTTTTCAGATCCTTGGGTTCGCGCTGTTCATCTTCTGGCTGCTGCTGATCGCTGG GGTCGTCGTTGAGTTCATCCGCTCGTTCAGCCGTGACTGGCGTCCCACCGGTGTCACCGTGGT GATCTTGGAGATCATCATGTCGATCACTGATCCGCCGGTGAAGGTGCTGCGCCGGCTGATCCC GCAACTCACGATCGGCGCGGTCCGGTTCGACCTGTCGATCATGGTGCTGCTGCTGGTTGCGTT CATCGGTATGCAACTGGCGTTTGGTGCTGCGGCCTGA

>Rv2147c - TB.seq 2406119:2406841 MW:27630

>emb|AL123456|MTBH37RV:c2406841 -2406116, Rv2147c SEQ ID NO:73

GTGAATAGTCACTGTAGTCACACCTTCATCACAGACAACAGATCTCCCAGGGCTAGAAGGGGTC ACGCAATGAGCACACTGCACAAGGTCAAGGCCTACTTCGGTATGGCTCCCATGGAGGATTACGA

CGACGAGTACTACGACGACCGCGCTCCCTCGCGCGGGTATGCGCGGCCCCGATTCGACGACG ACTACGGCCGCTACGATGGGCGCGACTACGACGACGCGCGCAGCGATTCACGCGGTGACCTG CGCGGTGAGCCGGCCGACTATCCACCACCGGGATATCGCGGCGGGTACGCGGACGAACCACG TTTCCGGCCCCGGGAGTTCGACCGCGCGGAGATGACACGGCCGCGCTTCGGATCGTGGCTGC GCAACTCCACCCGCGGCGCGCTAGCGATGGACCCCCGCCGGATGGCGATGATGTTCGAGGAT

GGCCATCCGCTCTCGAAGATCACCACGCTGCGGCCCAAGGACTACAGCGAGGCTCGCACCATC GGTGAGCGGTTCCGCGACGGCAGCCCGGTCATCATGGATCTGGTGTCGATGGACAACGCCGAT GCCAAGCGGCTGGTCGATTTCGCGGCCGGCCTGGCCTTCGCGCTGCGCGGCTCGTTCGACAA GGTCGCGACCAAGGTGTTCCTGCTCTCGCCTGCAGACGTCGATGTGTCCCCCGAGGAGCGCCG CAGGATCGCCGAAACCGGGTTCTACGCCTACCAATAG

>Rv2148c - TB.seq 2406841 :2407614 MW:27694 >emb|AL123456|MTBH37RV:c2407614-2406838, Rv2148c SEQ ID N0:74

ATGGCGGCGGATCTTTCGGCGTATCCAGACCGCGAATCGGAATTGACGCATGCGTTGGCGGCA ATGCGATCGCGACTTGCGGCGGCCGCGGAGGCGGCGGGTCGCAATGTCGGCGAAATTGAACT TCTACCGATTACCAAATTCTTTCCAGCAACCGATGTTGCGATTTTGTTTCGATTGGGTTGTCGGTC CGTTGGCGAATCGCGCGAACAGGAAGCTTCAGCCAAGATGGCCGAACTTAATCGGTTGTTGGC

GGCTGCCGAGTTGGGTCACTCGGGGGGTGTGCACTGGCACATGGTGGGCCGGATTCAACGCA ACAAAGCCGGGTCGCTGGCTCGCTGGGCGCACACCGCTCACTCGGTGGACAGCTCGCGGTTG GTGACCGCGCTGGATCGGGCGGTTGTTGCGGCGCTGGCCGAACACCGTCGTGGCGAGCGGCT GCGGGTTTACGTCCAGGTCAGCCTCGACGGTGACGGATCCCGGGGCGGCGTCGACAGCACGA CGCCCGGCGCCGTAGACCGGATTTGCGCGCAGGTGCAGGAGTCAGAGGGCCTCGAACTGGTC

GGGTTGATGGGCATTCCGCCGCTGGATTGGGACCCGGACGAGGCCTTTGACCGGCTGCAATCG GAGCACAACCGGGTGCGTGCGATGTTCCCGCACGCGATCGGTCTGTCGGCGGGCATGTCCAAC GACCTTGAAGTCGCCGTCAAACATGGTTCGACCTGTGTGCGTGTCGGTACCGCGCTATTGGGTC CGCGGCGGTTACGGTCACCGTGA

>Rv2150c ftsZ TB.seq 2408386:2409522 MW:38757 >emb|AL123456|MTBH37RV:c2409522-2408383, ftsZ SEQ ID NO:75

ATGACCCCCCCGCACAACTACCTGGCCGTCATCAAGGTCGTGGGTATCGGTGGTGGCGGTGTC AACGCCGTCAACCGAATGATCGAGCAGGGCCTCAAAGGCGTGGAATTCATCGCGATCAACACC GACGCCCAGGCGTTGTTGATGAGCGATGCCGACGTCAAACTCGACGTCGGCCGCGACTCCACC

CGCGGGCTGGGCGCCGGCGCCGATCCGGAGGTCGGCCGTAAGGCCGCCGAGGACGCCAAGG ACGAGATCGAAGAGCTGCTGCGCGGTGCCGACATGGTGTTTGTCACCGCCGGCGAGGGGGGC GGAACCGGCACCGGGGGGGCACCCGTCGTCGCCAGCATCGCCCGCAAGCTGGGCGCGTTGAC CGTCGGTGTGGTCACCCGGCCGTTCTCGTTCGAGGGCAAGCGACGCAGCAATCAGGCCGAAAA TGGCATCGCGGCGCTGCGGGAGAGTTGCGACACCCTCATCGTGATTCCCAACGACCGGTTGCT

GCAGATGGGAGATGCCGCGGTATCGCTGATGGATGCTTTCCGTAGCGCCGACGAGGTGCTGCT CAACGGCGTGCAGGGCATCACCGACCTGATTACCACCCCGGGTCTAATCAACGTCGACTTCGC CGACGTCAAGGGCATCATGTCCGGTGCCGGCACCGCACTGATGGGCATCGGCTCGGCCCGGG GCGAAGGCCGGTCGCTCAAAGCGGCCGAGATCGCCATCAACTCGCCGTTGCTGGAAGCCTCGA TGGAGGGCGCGCAAGGCGTGCTGATGTCGATCGCCGGCGGCAGCGACTTGGGCTTGTTCGAG

ATCAACGAGGCGGCCTCGTTGGTACAAGACGCCGCTCACCCCGATGCCAACATCATCTTCGGC ACCGTCATCGACGATTCGCTCGGTGACGAGGTGCGGGTGACCGTGATCGCGGCCGGCTTCGAC GTCAGCGGTCCCGGCCGCAAGCCGGTGATGGGCGAGACCGGCGGCGCCCACCGGATCGAGT CAGCCAAGGCAGGCAAGCTCACCTCGACCTTGTTCGAGCCGGTCGACGCCGTCAGCGTGCCGT TGCACACCAACGGCGCAACCCTGAGCATCGGCGGTGATGACGACGATGTCGACGTGCCGCCCT

TCATGCGCCGCTGA >Rv2152c murC TB.seq 2410639:2412120 MW:51146 >emb|AL123456|MTBH37RV:c2412120-2410636, murC SEQ ID NO:76

GTGAGCACCGAGCAGTTGCCGCCCGATCTGCGGCGGGTGCACATGGTCGGCATCGGCGGAGC TGGCATGTCGGGCATCGCCCGAATCCTGCTGGACCGCGGCGGGCTGGTCTCCGGGTCAGACG CCAAGGAGTCGCGCGGTGTGCATGCGCTGCGGGCGCGGGGCGCGTTGATCCGGATCGGACAC

GACGCGTCGTCGCTGGACCTGTTGCCCGGTGGCGCCACGGCGGTCGTCACTACCCATGCCGC CATCCCCAAAACCAACCCCGAGCTCGTCGAAGCGAGGCGCCGCGGCATTCCCGTGGTGCTGCG GCCGGCCGTGCTGGCCAAGTTGATGGCCGGGCGCACCACATTGATGGTCACCGGCACGCACG GCAAGACAACGACGACGTCCATGCTGATCGTCGCCCTGCAGCACTGCGGGCTTGACCCGTCCT TTGCGGTCGGCGGTGAGCTGGGGGAGGCCGGTACCAACGCCCATCACGGCAGTGGCGACTGT

TTCGTCGCCGAAGCCGACGAAAGCGATGGCTCGCTGTTGCAGTACACACCCCACGTCGCGGTG ATCACCAACATCGAGTCCGATCACCTGGACTTCTACGGCAGCGTCGAGGCGTATGTTGCGGTGT TCGACTCCTTCGTGGAGCGCATTGTCCCCGGGGGTGCGCTGGTGGTGTGCACTGACGACCCCG GAGGGGCCGCGCTGGCTCAGCGCGCGACTGAGCTGGGAATTCGAGTGCTGCGATACGGGTCG GTGCCGGGTGAGACCATGGCAGCCACGTTGGTCTCGTGGCAGCAACAGGGGGTCGGCGCGGT

CGCACATATCCGGTTGGCCTCAGAACTAGCCACAGCACAGGGTCCCCGCGTGATGCGGCTGTC GGTGCCCGGGCGACACATGGCGCTCAACGCGCTGGGAGCGCTGCTGGCCGCGGTGCAGATCG GCGCCCCGGCCGACGAGGTGCTCGACGGGCTGGCCGGCTTCGAAGGAGTGCGGCGACGATTC GAACTGGTTGGGACCTGCGGCGTCGGAAAGGCGTCGGTGCGCGTGTTCGATGACTACGCCCAC CACCCGACGGAGATCAGCGCGACACTGGCGGCGGCGCGCATGGTGCTCGAACAGGGCGACGG

TGGCCGCTGCATGGTTGTGTTTCAACCCCATTTGTATTCGCGGACAAAGGCATTCGCTGCTGAG TTTGGGCGTGCGCTGAATGCCGCTGACGAGGTGTTCGTACTCGACGTCTACGGAGCTCGTGAA CAACCGCTGGCCGGTGTCAGCGGAGCCAGCGTCGCTGAGCACGTCACTGTGCCGATGCGCTA CGTCCCGGATTTTTCGGCGGTCGCACAGCAAGTGGCCGCCGCCGCTAGTCCGGGCGACGTCAT CGTCACGATGGGTGCCGGAGACGTGACCTTGCTGGGCCCGGAAATCCTGACCGCCCTTCGGGT

CCGGGCCAACCGAAGCGCCCCCGGCCGTCCGGGGGTGCTGGGATGA

>Rv2153c murG TB.seq 2412120:2413349 MW:41829 >emb|AL123456|MTBH37RV:c2413349-2412117, murG SEQ ID NO:77 GTGAAGGACACGGTCAGCCAGCCGGCCGGCGGGCGCGGGGCAACGGCGCCCCGGCCCGCCG

ATGCCGCCTCGCCGTCTTGTGGTTCCTCGCCGTCTGCTGATTCCGTGTCGGTCGTTCTCGCCGG CGGCGGGACCGCCGGGCACGTCGAGCCCGCCATGGCCGTCGCCGACGCCTTGGTCGCGTTGG ATCCGCGCGTCCGGATTACCGCGTTGGGCACCCTCCGTGGACTAGAGACCAGGCTGGTGCCCC AGCGCGGCTACCACCTGGAGCTGATCACGGCGGTGCCGATGCCGCGCAAGCCCGGCGGCGAC CTGGCCCGGCTGCCGTCGCGGGTGTGGCGCGCCGTCCGGGAGGCCCGGGACGTGCTCGACG

ATGTCGACGCCGACGTCGTCGTCGGTTTCGGTGGGTACGTCGCGCTACCGGCTTACCTAGCCG CTCGCGGCCTGCCTTTGCCGCCCCGGCGCCGGCGCCGGATCCCGGTGGTGATCCACGAAGCC AACGCCAGGGCGGGACTGGCCAACCGGGTCGGCGCCCATACCGCGGACCGGGTGCTCTCCGC GGTGCCGGATTCCGGGCTGCGGCGCGCCGAGGTGGTTGGGGTCCCGGTCCGTGCGTCGATCG CCGCGCTGGACCGCGCGGTGCTGCGAGCCGAGGCGCGGGCACACTTCGGCTTCCCCGACGAC GCGCGGGTGCTGCTGGTGTTCGGGGGTTCGCAGGGCGCGGTCTCGCTCAACCGGGCGGTGTC CGGCGCCGCCGCCGACCTGGCCGCCGCCGGTGTTTGCGTGCTGCATGCCCATGGACCCCAGA

ACGTGCTGGAGTTGCGCCGTCGGGCTCAAGGTGACCCACCGTACGTGGCGGTGCCCTATTTGG ACCGGATGGAGCTGGCCTACGCCGCCGCCGATCTGGTGATCTGCCGGGCCGGGGCGATGACG GTCGCCGAAGTATCCGCCGTCGGTCTGCCGGCCATCTACGTGCCGCTGCCGATCGGCAACGGT GAACAGCGGCTGAATGCGTTGCCGGTAGTCAATGCCGGCGGCGGCATGGTGGTCGCCGACGC CGCCCTGACCCCCGAGTTGGTGGCCCGCCAGGTTGCCGGGCTGCTCACCGACCCCGCGCGGC

TGGCCGCGATGACCGCGGCCGCAGCCAGGGTGGGACATCGCGATGCCGCGGGCCAGGTGGC CCGGGCCGCGCTGGCCGTCGCCACCGGGGCCGGTGCCAGGACAACGACGTGA

>Rv2154c ftsW TB.seq 2413349:2414920 MW:56306 >emb|AL123456|MTBH37RV:c2414920-2413346, ftsW SEQ ID NO:78

GTGCTAACCCGGTTGCTGCGTCGGGGCACCAGCGACACCGACGGCTCCCAGACTCGAGGGGC CGAGCCGGTCGAGGGGCAGCGGACGGGCCCGGAAGAAGCCTCTAACCCGGGTTCGGCGAGG CCCCGCACCCGTTTCGGTGCCTGGCTGGGCCGTCCGATGACCTCGTTTCACCTCATCATCGCC GTTGCCGCATTGCTGACCACCCTTGGACTGATCATGGTGCTGTCGGCATCGGCGGTGCGGTCC TACGACGACGACGGATCGGCTTGGGTGATCTTCGGCAAGCAGGTCTTGTGGACGCTTGTGGGT

CTTATCGGCGGCTATGTCTGTCTGCGGATGTCGGTGCGGTTCATGCGGCGCATCGCCTTCTCCG GTTTCGCGATCACCATCGTGATGCTGGTGCTGGTGCTGGTGCCGGGGATCGGCAAGGAGGCCA ACGGCTCGCGCGGCTGGTTCGTGGTCGCGGGCTTCTCGATGCAGCCCTCTGAGCTGGCTAAGA TGGCGTTCGCCATCTGGGGAGCGCATCTGCTGGCCGCCCGGCGCATGGAACGGGCTTCACTG CGCGAGATGCTGATTCCACTGGTGCCGGCCGCCGTCGTTGCGCTGGCGCTGATCGTGGCCCAG

CCCGACCTCGGACAGACCGTGTCGATGGGCATCATCTTGTTGGGCCTGCTGTGGTATGCGGGG CTGCCGCTGCGCGTCTTCCTCAGCTCACTGGCGGCGGTCGTCGTCTCGGCCGCCATCCTGGCG GTGTCCGCGGGCTACCGATCCGACCGGGTGCGGTCGTGGCTCAACCCCGAAAACGATCCGCAA GACTCCGGCTACCAGGCCCGACAGGCAAAGTTCGCGCTGGCTCAAGGTGGCATTTTCGGCGAC GGTCTGGGCCAAGGCGTGGCCAAGTGGAACTACTTGCCCAACGCCCACAACGACTTCATTTTCG

CCATCATCGGCGAAGAGCTGGGTCTCGTCGGCGCGCTCGGACTGCTGGGGCTATTCGGATTGT TCGCCTACACCGGCATGCGCATCGCTAGCCGGTCCGCCGACCCGTTCCTGCGGCTGCTGACCG CCACCACGACACTGTGGGTGCTGGGACAGGCGTTCATCAACATCGGCTATGTGATCGGGCTGC TGCCCGTCACCGGCCTGCAGCTGCCGCTCATCTCCGCCGGTGGAACCTCCACGGCCGCAACAC TTTCGCTGATAGGCATCATCGCCAACGCGGCTCGCCACGAACCGGAGGCGGTGGCCGCGCTG

CGGGCTGGGCGCGACGACAAGGTGAACCGGTTGCTGCGGCTGCCGCTGCCCGAGCCGTATCT GCCCCCTCGTCTCGAGGCGTTTCGTGACCGCAAGCGCGCCAACCCGCAACCGGCCCAAACGCA GCCCGCGCGGAAGACCCCCCGCACGGCGCCCGGACAGCCTGCCCGGCAGATGGGCCTGCCC CCGCGACCCGGCTCGCCCCGCACGGCCGATCCGCCGGTTCGTCGATCAGTGCATCATGGAGCT GGCCAGCGGTACGCGGGCCAGCGTCGCACACGGCGCGTTCGGGCATTGGAAGGTCAGCGTTA CGGGTGA

>Rv2155c murD TB.seq 2414935:2416392 MW:49314 >emb|AL123456|MTBH37RV:c2416392-2414932, murD SEQ ID NO:79

GTGCTTGACCCTCTGGGGCCGGGTGCGCCCGTGTTGGTAGCCGGTGGCCGGGTGACCGGTCA GGCGGTGGCCGCGGTGCTGACTCGGTTTGGTGCGACGCCGACGGTGTGCGACGACGATCCGG TCATGCTGCGACCGCACGCCGAACGTGGGCTGCCGACCGTTAGTTCCTCGGACGCGGTGCAGC

AGATAACCGGGTATGCGCTGGTGGTCGCCAGTCCCGGCTTCTCGCCCGCAACCCCGCTACTGG CCGCGGCCGCGGCGGCGGGGGTGCCGATCTGGGGTGACGTGGAGTTAGCCTGGCGGCTAGA CGCAGCGGGCTGCTACGGACCGCCGCGCAGCTGGCTGGTGGTGACCGGCACCAACGGCAAGA CCACCACGACGTCGATGCTGCACGCCATGCTGATCGCCGGTGGCCGCCGCGCCGTGCTGTGC GGCAATATCGGCAGTGCGGTGCTGGATGTGCTGGACGAGCCGGCCGAGCTGCTGGCCGTGGA

GTTGTCCAGTTTCCAGCTGCACTGGGCGCCGTCGCTGCGGCCCGAGGCCGGCGCGGTGCTCA ACATTGCCGAAGACCACCTGGACTGGCATGCCACGATGGCCGAATACACCGCGGCCAAGGCCC GGGTGCTGACCGGCGGGGTAGCGGTGGCCGGGCTGGATGACAGCCGAGCGGCCGCACTGCT GGACGGCTCACCGGCGCAGGTGCGGGTCGGCTTCCGGCTCGGCGAGCCGGCCGCGCGGGAA CTGGGCGTGCGCGACGCCCACCTGGTCGATCGCGCCTTCTCCGACGACTTGACGCTGCTGCCG

GTCGCGTCGATACCGGTGCCAGGTCCGGTCGGCGTGCTTGACGCCCTGGCCGCGGCGGCGCT GGCCCGCTCGGTCGGGGTGCCCGCCGGTGCGATCGCCGACGCGGTCACGTCGTTTCGAGTGG GCCGACACCGCGCCGAGGTGGTGGCCGTTGCCGACGGCATCACCTACGTGGACGACTCCAAG GCCACCAACCCGCACGCCGCGCGGGCTTCGGTGCTTGCATACCCGAGGGTGGTATGGATCGC CGGTGGCCTGCTCAAGGGCGCGTCGCTTCACGCCGAGGTTGCGGCGATGGCGTCGCGGCTGG

TCGGTGCGGTGCTGATCGGCCGGGATCGCGCAGCGGTTGCCGAGGCGTTATCACGACACGCG CCCGATGTCCCAGTCGTTCAGGTTGTGGCAGGCGAGGATACTGGTATGCCTGCGACTGTTGAG GTTCCTGTTGCTTGTGTTCTAGATGTGGCAAAAGATGACAAAGCCGGTGAGACCGTTGGCGCTG CCGTGATGACCGCTGCGGTGGCCGCGGCCCGGCGGATGGCCCAACCCGGTGACACCGTGCTG CTGGCACCGGCCGGCGCCTCATTCGACCAGTTCACCGGTTATGCCGACCGGGGCGAGGCATTC

GCGACCGCGGTCCGCGCGGTGATCCGGTAG

>Rv2156c murX TB.seq 2416397:2417473 MW:37714 >emb|AL123456|MTBH37RV:c2417473-2416394, murX SEQ ID NO:80 ATGAGGCAGATCCTTATCGCCGTTGCCGTAGCGGTGACGGTGTCCATCTTGCTGACCCCGGTG

CTGATCCGGTTGTTCACTAAGCAGGGCTTCGGCCACCAGATCCGTGAGGATGGCCCGCCCAGC CACCACACCAAGCGCGGTACGCCGTCGATGGGCGGGGTGGCGATTCTGGCCGGCATCTGGGC GGGCTACCTGGGCGCCCACCTAGCGGGCCTGGCGTTTGACGGTGAAGGCATCGGCGCATCGG GTCTGTTGGTGCTGGGCCTAGCCACCGCTTTGGGCGGCGTCGGGTTCATCGACGATCTGATCA AGATCCGCAGGTCGCGCAATCTCGGGTTGAACAAGACGGCCAAGACCGTCGGGCAGATCACCT CCGCCGTGCTGTTTGGCGTGCTGGTGCTGCAGTTCCGGAATGCTGCCGGCCTGACACCGGGCA GCGCGGATCTGTCCTACGTGCGTGAGATCGCCACCGTCACATTGGCGCCGGTGCTGTTCGTGT

TGTTCTGCGTGGTCATCGTCAGCGCCTGGTCGAACGCGGTCAACTTCACCGATGGCCTGGACG GGCTGGCCGCCGGCACCATGGCGATGGTCACCGCCGCCTACGTGCTGATCACCTTCTGGCAGT ACCGCAACGCGTGCGTGACGGCGCCGGGCCTGGGCTGCTACAACGTGCGCGACCCGCTGGAC CTGGCGCTCATCGCGGCCGCAACCGCTGGCGCCTGCATCGGTTTTTTGTGGTGGAACGCCGCG CCCGCCAAGATCTTCATGGGTGACACTGGGTCGCTGGCGTTGGGCGGCGTCATCGCGGGGTTG

TCGGTGACCAGCCGCACCGAGATCCTTGCGGTGGTGCTGGGTGCGCTGTTCGTCGCCGAGATC ACCTCGGTGGTGTTGCAAATCCTGACCTTCCGGACCACCGGGCGCCGGATGTTTCGGATGGCG CCCTTCCACCACCATTTCGAGTTGGTCGGTTGGGCTGAAACCACGGTCATCATCCGGTTCTGGC TGCTCACCGCGATCACCTGCGGTCTGGGCGTGGCCTTGTTCTACGGTGAGTGGCTTGCCGCGG TCGGTGCCTGA

>Rv2157c murF TB.seq 2417473:2419002 MW:51634 >emb|AL123456|MTBH37RV:c2419002-2417470, murF SEQ ID NO:81 ATGATCGAGCTGACCGTCGCGCAGATCGCCGAGATCGTCGGGGGCGCAGTGGCCGATATCTCC CCGCAAGACGCCGCGCACCGCCGCGTCACCGGGACCGTCGAGTTCGACTCGCGCGCCATCGG

CCCGGGCGGGCTGTTCCTCGCCCTGCCGGGGGCGCGCGCCGACGGCCACGACCATGCCGCG TCGGCGGTAGCCGCGGGCGCCGCCGTCGTGCTGGCCGCCCGCCCGGTGGGGGTGCCGGCCA TCGTGGTTCCGCCAGTGGCCGCGCCGAACGTATTGGCCGGCGTCCTCGAGCACGACAACGAC GGGTCGGGGGCGGCGGTGCTGGCCGCGCTGGCCAAGCTGGCCACCGCGGTGGCCGCGCAGT TGGTGGCCGGCGGGCTCACCATCATCGGGATCACCGGCTCGTCGGGCAAGACGTCGACCAAG

GACCTGATGGCCGCCGTGCTGGCCCCGCTGGGGGAGGTGGTGGCCCCGCCCGGATCGTTCAA CAACGAGCTGGGTCACCCGTGGACGGTGCTGCGCGCGACGCGGCGCACCGACTACCTGATTTT GGAGATGGCGGCACGCCATCACGGCAACATCGCCGCGCTCGCCGAGATCGCGCCCCCGTCGA TCGGAGTCGTGCTCAACGTCGGCACCGCACATTTGGGTGAGTTCGGCTCCCGCGAGGTCATCG CACAGACCAAAGCCGAACTGCCGCAGGCTGTTCCGCATTCCGGAGCGGTCGTCCTCAACGCTG

ATGACCCCGCGGTGGCGGCGATGGCCAAGCTGACCGCGGCCCGGGTGGTGCGGGTCAGCCG GGACAACACCGGTGACGTTTGGGCGGGGCCGGTGTCGCTGGACGAATTGGCCAGGCCGCGCT TTACGCTGCATGCCCACGATGCCCAAGCCGAGGTCCGACTCGGGGTCTGCGGCGACCACCAG GTCACTAACGCGCTGTGCGCCGCGGCGGTCGCGCTGGAGTGTGGGGCCAGCGTTGAACAGGT CGCGGCCGCGCTGACCGCGGCGCCGCCGGTGTCGCGGCATCGGATGCAGGTGACCACCCGC

GGCGACGGGGTGACGGTGATCGACGACGCCTACAACGCCAACCCCGACTCCATGCGGGCCGG GCTGCAGGCGCTGGCCTGGATCGCGCACCAACCCGAGGCCACCCGCCGCAGCTGGGCGGTGC TGGGTGAGATGGCCGAGCTGGGTGAGGACGCGATAGCCGAGCACGATCGCATCGGCCGGCTC GCGGTGCGCTTAGATGTGTCTCGACTCGTTGTCGTGGGAACCGGGAGGTCGATCAGCGCCATG CACCACGGAGCGGTCCTGGAGGGGGCGTGGGGCTCGGGGGAAGCCACTGCTGATCACGGTGC GGATCGCACGGCCGTCAATGTGGCCGACGGTGACGCCGCCCTGGCACTACTGCGCGCCGAGC TGCGACCCGGGGATGTGGTCTTGGTCAAGGCCTCGAACGCGGCCGGGCTGGGTGCGGTGGCC

GATGCATTGGTCGCAGACGACACATGCGGGAGTGTGCGCCCATGA

>Rv2158c murE TB.seq 2419002:2420606 MW:55310 >emb|AL123456|MTBH37RV:c2420606-2418999, murE SEQ ID NO:82 GTGTCATCGCTGGCCCGAGGGATCTCGCGGCGGCGAACGGAGGTGGCGACACAGGTGGAGGC

TGCGCCCACTGGCTTGCGCCCCAACGCCGTCGTGGGCGTTCGGTTGGCCGCACTGGCCGATCA GGTCGGCGCGGCCCTGGCCGAGGGTCCAGCTCAGCGTGCCGTCACCGAGGACCGGACGGTCA CCGGGGTCACGCTGCGCGCCCAGGACGTGTCACCCGGTGACCTGTTCGCCGCCCTGACCGGC TCGACCACCCACGGGGCCCGCCACGTCGGCGACGCGATCGCACGCGGCGCCGTCGCGGTGCT CACCGACCCCGCCGGGGTCGCCGAGATCGCCGGACGAGCGGCCGTGCCCGTGTTGGTGCACC

CCGCACCCCGCGGCGTGCTCGGCGGCTTGGCCGCCACCGTGTACGGGCATCCGTCCGAGCGG TTGACGGTTATCGGGATCACCGGAACGTCCGGCAAGACCACCACCACCTATCTGGTCGAGGCC GGGTTACGGGCTGCCGGACGCGTCGCCGGGCTGATCGGCACCATCGGCATCCGCGTCGGCGG CGCCGACCTTCCCAGCGCGCTGACCACCCCGGAGGCCCCCACGCTGCAGGCGATGCTGGCGG CGATGGTCGAACGCGGGGTGGACACCGTGGTCATGGAGGTGTCCAGCCACGCGCTGGCGCTG

GGCCGGGTGGACGGCACCCGGTTCGCCGTCGGCGCCTTCACCAATCTCTCCCGTGACCACCTG GATTTCCACCCCAGCATGGCCGACTACTTCGAGGCCAAGGCGTCATTGTTCGATCCGGACTCGG CACTGCGCGCCCGCACCGCCGTGGTGTGCATCGACGACGACGCCGGGCGCGCGATGGCGGC GCGGGCCGCCGACGCGATCACCGTCAGCGCCGCCGACCGGCCCGCACACTGGCGCGCCACG GATGTGGCGCCCACGGACGCGGGCGGGCAACAATTCACCGCCATCGACCCCGCCGGCGTAGG

GCATCACATCGGAATCCGGCTACCGGGCCGCTACAACGTCGCCAATTGCCTGGTCGCCCTGGC GATTCTGGACACCGTCGGGGTCTCCCCGGAACAGGCGGTGCCGGGCCTGCGTGAGATCCGGG TCCCGGGGCGGCTCGAGCAGATCGACCGCGGCCAGGGCTTTCTCGCGCTGGTCGACTACGCG CACAAACCGGAAGCGCTGCGGTCGGTGCTGACCACCTTGGCGCACCCGGACCGCCGGCTGGC GGTGGTGTTCGGCGCCGGCGGCGATCGTGACCCGGGCAAGCGGGCCCCGATGGGCCGGATA

GCCGCGCAGCTGGCCGACTTGGTGGTCGTCACCGACGACAACCCGCGTGACGAAGATCCCAC GGCGATCCGCCGCGAAATCCTGGCTGGGGCGGCCGAAGTCGGCGGTGATGCCCAGGTCGTCG AGATCGCAGACCGGCGGGACGCGATCCGGCACGCGGTTGCCTGGGCGCGCCCCGGCGACGT GGTGCTCATCGCCGGCAAAGGCCACGAGACCGGGCAACGCGGCGGCGGGCGGGTCCGCCCG TTCGACGACCGGGTGGAGCTGGCTGCCGCGCTAGAGGCCCTCGAGCGGCGCGCATGA

>Rv2159c - TB.seq 2420632:2421663 MW:36377 >emb|AL123456|MTBH37RV:c2421663-2420629, Rv2159c SEQ ID NO:83

ATGAAATTTGTCAACCATATTGAGCCCGTCGCGCCCCGCCGAGCCGGCGGCGCGGTCGCCGAG GTCTATGCCGAGGCCCGCCGCGAGTTCGGCCGGCTGCCCGAGCCGCTCGCCATGCTGTCCCC GGACGAGGGACTGCTCACCGCCGGCTGGGCGACGTTGCGCGAGACACTGCTGGTGGGCCAGG TGCCGCGTGGCCGCAAGGAAGCCGTCGCCGCCGCCGTCGCGGCCAGCCTGCGCTGCCCCTGG

TGCGTCGACGCACACACCACCATGCTGTACGCGGCAGGCCAAACCGACACCGCCGCGGCGAT CTTGGCCGGCACAGCACCTGCCGCCGGTGACCCGAACGCGCCGTATGTGGCGTGGGCGGCAG GAACCGGGACACCGGCGGGACCGCCGGCACCGTTCGGCCCGGATGTCGCCGCCGAATACCTG GGCACCGCGGTGCAATTCCACTTCATCGCACGCCTGGTCCTGGTGCTGCTGGACGAAACCTTC CTGCCGGGGGGCCCGCGCGCCCAACAGCTCATGCGCCGCGCCGGTGGACTGGTGTTCGCCCG

CAAGGTGCGCGCGGAGCATCGGCCGGGCCGCTCCACCCGCCGGCTCGAGCCGCGAACGCTG CCCGACGATCTGGCATGGGCAACACCGTCCGAGCCCATAGCAACCGCGTTCGCCGCGCTCAGC CACCACCTGGACACCGCGCCGCACCTGCCGCCACCGACTCGTCAGGTGGTCAGGCGGGTCGT GGGGTCGTGGCACGGCGAGCCAATGCCGATGAGCAGTCGCTGGACGAACGAGCACACCGCCG AGCTGCCCGCCGACCTGCACGCGCCCACCCGTCTTGCCCTGCTGACCGGCCTGGCCCCGCAT

CAGGTGACCGACGACGACGTCGCCGCGGCCCGATCCCTGCTCGACACCGATGCGGCGCTGGT TGGCGCCCTGGCCTGGGCCGCCTTCACCGCCGCGCGGCGCATCGGCACCTGGATCGGCGCCG CCGCCGAGGGCCAGGTGTCGCGGCAAAACCCGACTGGGTGA

>Rv2163c pbpB TB.seq 2425049:2427085 MW:72506

>emb|AL123456|MTBH37RV:c2427085-2425046, pbpB SEQ ID NO:84

GTGAGCCGCGCCGCCCCCAGGCGGGCCAGTCAGTCGCAGTCGACGCGACCGGCGCGCGGTTT GCGCCGGCCACCGGGAGCCCAGGAGGTTGGGCAACGCAAACGGCCCGGCAAAACGCAGAAAG CCCGGCAAGCCCAGGAAGCCACGAAATCCCGCCCTGCGACACGGTCAGACGTCGCACCCGCG GGTCGCTCGACTCGTGCGAGGCGCACCCGGCAGGTGGTGGACGTCGGGACGCGCGGTGCGTC

GTTCGTCTTTCGGCATCGGACCGGAAACGCGGTCATCTTGGTGTTGATGTTGGTCGCGGCAACA CAATTGTTCTTTCTGCAGGTATCACATGCCGCGGGCCTGCGTGCGCAGGCGGCCGGCCAACTC AAGGTCACCGACGTCCAGCCAGCGGCTCGCGGCAGCATCGTCGACCGCAACAATGACCGGCTC GCGTTCACCATCGAGGCGCGTGCCCTGACGTTCCAGCCGAAGCGGATTCGGCGGCAATTGGAA GAGGCCAGGAAGAAGACGTCGGCTGCACCCGACCCGCAGCAGCGCCTGCGCGATATCGCCCA

GGAGGTCGCCGGCAAGCTGAACAACAAGCCAGATGCCGCGGCCGTGCTGAAGAAGCTGCAAA GCGACGAGACCTTCGTCTACTTGGCGCGTGCGGTCGACCCGGCTGTCGCCAGCGCGATCTGCG CGAAGTATCCCGAGGTCGGTGCGGAAAGACAGGATCTGCGTCAGTACCCGGGTGGGTCGCTG GCGGCAAACGTCGTCGGTGGCATCGACTGGGATGGTCATGGGCTGCTGGGTCTGGAGGACTCC CTGGATGCGGTGCTGGCCGGAACCGACGGATCGGTCACCTACGACCGTGGGTCAGACGGCGT

CGTCATCCCCGGCAGCTACCGGAATCGGCACAAGGCGGTCCACGGTTCCACCGTCGTGCTCAC CCTCGACAACGACATCCAGTTCTACGTGCAGCAGCAGGTGCAGCAGGCCAAGAACCTATCGGG GGCTCACAACGTCTCGGCCGTCGTCCTGGACGCCAAGACCGGCGAGGTGCTCGCGATGGCCA ACGACAACACCTTCGACCCGTCGCAAGACATCGGGCGCCAGGGCGACAAGCAGTTGGGCAACC CGGCGGTGTCGTCGCCCTTCGAGCCGGGCTCGGTGAACAAGATCGTCGCCGCGTCCGCGGTC ATCGAGCACGGGTTGAGCAGCCCCGACGAGGTGCTACAGGTGCCTGGCTCGATCCAGATGGG CGGTGTTACCGTGCATGACGCTTGGGAGCACGGCGTGATGCCCTATACCACCACGGGGGTGTT

CGGAAAGTCCTCCAACGTCGGCACGCTGATGCTTTCCCAACGTGTCGGACCGGAACGCTATTAC GATATGCTCCGCAAGTTCGGGTTGGGACAGCGCACCGGCGTGGGCCTGCCCGGTGAGAGCGC CGGACTGGTGCCGCCAATCGACCAGTGGTCGGGCAGTACGTTCGCTAATCTTCCTATTGGCCAA GGTCTTTCGATGACTTTGCTGCAGATGACCGGCATGTACCAGGCCATCGCCAACGATGGAGTGC GGGTACCCCCACGCATTATCAAGGCCACCGTCGCACCCGACGGCAGCCGAACCGAAGAACCGC

GCCCCGACGACATTCGCGTGGTGTCGGCGCAGACCGCCCAGACCGTGCGCCAGATGCTGCGT GCCGTGGTGCAACGCGATCCGATGGGCTACCAGCAGGGTACCGGGCCGACGGCCGGGGTGCC CGGCTATCAGATGGCCGGCAAGACCGGTACCGCGCAGCAGATCAACCCTGGCTGCGGCTGCTA CTTCGACGACGTGTATTGGATCACCTTCGCCGGAATCGCCACTGCCGACAATCCCCGCTACGTG ATCGGCATCATGTTGGACAACCCGGCGCGCAACTCCGACGGCGCGCCTGGGCACTCGGCCGC

CCCGCTGTTCCACAACATCGCGGGCTGGCTGATGCAGCGCGAAAACGTCCCGCTGTCACCCGA TCCCGGGCCTCCTTTGGTCTTGCAGGCCACCTAG

>Rv2165c - TB.seq 2428236:2429423 MW:42498 >emb|AL123456|MTBH37RV:c2429423-2428233, Rv2165c SEQ ID NO:85

GTGCAAACCCGTGCACCGTGGTCTCTGCCCGAAGCGACCCTGGCGTACTTCCCCAACGCCAGG TTCGTGTCTTCGGACAGGGACCTCGGTGCAGGGGCGGCGCCTGGAATAGCCGCGTCCCGAAGT ACGGCTTGCCAGACCTGGGGAGGTATCACGGTGGCTGATCCAGGTTCGGGGCCAACCGGTTTC GGTCATGTGCCGGTATTGGCGCAACGTTGCTTCGAACTGCTTACCCCCGCACTAACCCGCTACT ATCCAGACGGCTCGCAGGCGGTCCTTCTCGACGCGACCATCGGCGCGGGCGGGCATGCGGAG

CGGTTTTTGGAGGGATTGCCGGGTCTGCGCCTGATCGGGCTCGACCGTGACCCAACCGCTCTG GACGTCGCGCGGTCTCGGCTGGTGCGATTCGCTGACCGACTTACCCTGGTGCACACCCGCTAT GACTGTCTGGGCGCAGCGCTGGCTGAATCCGGTTATGCCGCAGTGGGATCAGTCGACGGAATC CTGTTCGATCTCGGCGTCTCATCCATGCAGCTCGACCGCGCCGAGCGGGGCTTCGCCTACGCC ACGGACGCGCCATTGGACATGCGGATGGACCCGACGACGCCGTTGACCGCAGCTGACATTGTC

AACACTTACGACGAGGCGGCACTAGCCGACATCCTGCGTCGCTACGGAGAGGAGCGGTTTGCT CGGCGCATCGCTGCCGGTATCGTCCGCCGACGCGCAAAAACCCCGTTCACCTCGACCGCCGAA CTGGTTGCCCTGCTGTACCAGGCGATTCCAGCTCCGGCCCGGCGTGTCGGCGGGCATCCAGCC AAGCGAACATTCCAGGCGCTGCGCATCGCGGTCAACGATGAGCTGGAATCGCTGCGCACGGCC GTTCCTGCCGCGCTGGATGCCCTCGCTATCGGTGGGCGCATCGCGGTGCTGGCCTACCAGTCG

CTAGAGGACAGGATCGTCAAACGGGTGTTCGCCGAGGCAGTCGCGTCGGCCACCCCTGCGGG ACTTCCGGTCGAACTTCCCGGCCATGAGCCGCGATTCCGTTCGTTAACGCACGGCGCCGAACG AGCGAGTGTGGCTGAGATCGAACGCAATCCCCGCAGTACTCCAGTGCGGTTGCGGGCCCTGCA ACGAGTCGAGCACCGGGCGCAATCGCAGCAATGGGCAACCGAGAAGGGTGATTCATGA

>Rv2166c - TB.seq 2429428:2429856 MW:15912 >emb|AL123456|MTBH37RV:c2429856-2429425, Rv2166c SEQ ID NO:86

ATGTTTCTCGGCACCTACACGCCCAAACTCGACGACAAGGGGCGGCTGACGCTGCCGGCCAAG TTTCGCGACGCGTTGGCAGGGGGGTTGATGGTCACCAAGAGCCAAGATCACAGCCTGGCCGTT TACCCGCGGGCGGCGTTCGAGCAGCTGGCGCGCCGGGCCAGCAAGGCGCCACGAAGCAACC CCGAGGCGAGAGCGTTCCTACGTAATCTCGCCGCCGGTACCGACGAACAGCATCCCGACAGTC AAGGCCGGATCACCTTGTCGGCCGACCACCGCCGCTACGCAAGCCTTTCCAAGGACTGTGTGG

TGATCGGCGCGGTCGACTATCTCGAGATCTGGGATGCGCAAGCCTGGCAGAACTACCAACAAAT CCATGAAGAGAACTTCTCCGCGGCCAGCGATGAAGCACTCGGTGACATCTTCTGA

>Rv2197c - TB.seq 2461505:2462146 MW:22481 >emb|AL123456|MTBH37RV:c2462146-2461502, Rv2197c SEQ ID NO:87

ATGGTGAGCAGATATTCCGCATACCGGCGTGGGCCGGATGTAATCTCGCCGGACGTCATCGAT CGCATCCTGGTTGGGGCATGTGCCGCGGTGTGGCTGGTGTTCACCGGCGTGTCGGTGGCCGC CGCTGTCGCCCTGATGGACCTGGGTAGGGGCTTCCACGAGATGGCCGGAAACCCGCACACCAC GTGGGTGCTGTACGCCGTAATTGTGGTCTCCGCACTGGTCATCGTGGGCGCGATACCGGTGCT GTTGCGAGCTCGCCGCATGGCTGAGGCCGAGCCCGCGACGAGGCCGACGGGTGCATCCGTGC

GGGGCGGGCGATCGATCGGATCCGGGCATCCGGCGAAACGCGCTGTGGCCGAGTCGGCACCC GTACAGCACGCGGATGCATTCGAGGTGGCCGCCGAGTGGTCCAGTGAGGCGGTGGACCGGAT CTGGTTGCGCGGGACAGTCGTGTTGACCAGTGCGATTGGCATTGCGTTGATTGCCGTGGCGGC GGCGACCTACCTCATGGCGGTCGGTCACGACGGGCCATCTTGGATCAGCTACGGGTTGGCCGG GGTGGTCACCGCGGGCATGCCGGTGATCGAGTGGCTATACGCTCGGCAGCTGCGCCGGGTGG

TGGCGCCCCAGTCCAGTTAG

>Rv2198c - TB.seq 2462149:2463045 MW:30955 >emb|AL123456|MTBH37RV:c2463045-2462146, mmpS3 SEQ ID NO:88 ATGAGCGGGCCGAATCCCCCGGGACGGGAACCTGACGAACCCGAATCGGAACCCGTCAGCGA

CACGGGCGACGAACGGGCTTCCGGCAACCACTTGCCGCCCGTCGCCGGGGGCGGCGACAAAC TGCCCAGTGACCAGACGGGCGAGACCGACGCATATTCTCGGGCATACTCTGCCCCGGAATCCG AGCACGTCACCGGCGGCCCGTATGTGCCAGCCGATCTCAGGCTCTATGACTACGACGACTATG AGGAGTCGTCCGACCTGGACGACGAACTGGCCGCTCCGCGCTGGCCGTGGGTGGTCGGTGTC GCCGCCATAATTGCCGCCGTTGCGCTCGTGGTTTCGGTGTCGTTGCTCGTCACGCGACCACATA

CCAGCAAACTCGCCACCGGCGACACTACGTCCTCTGCACCGCCCGTGCAGGACGAAATCACGA CCACCAAGCCGGCGCCGCCACCGCCGCCACCAGCCCCACCGCCCACCACCGAGATCCCGACA GCGACGGAGACACAGACGGTCACTGTGACGCCGCCACCACCGCCCCCACCGGCGACAACCAC GGCGCCGCCGCCGGCGACCACCACAACGGCGGCGGCACCGCCGCCCACGACCACCACGCCG ACCGGTCCGCGGCAAGTCACCTATTCGGTGACCGGTACCAAGGCGCCGGGTGACATTATCTCG GTGACTTACGTCGATGCCGCCGGGCGCCGACGGACACAGCACAATGTGTACATCCCGTGGTCC ATGACGGTCACCCCGATCTCGCAATCCGACGTTGGCTCGGTGGAGGCCTCCAGCCTTTTCCGG

GTCAGCAAACTCAACTGCTCGATCACCACGAGCGACGGAACGGTGCTCTCATCGAACTCCAACG ATGGACCGCAAACGAGCTGCTGA >Rv2199c - TB.seq 2463234:2463650 MW:14866 >emb|AL123456|MTBH37RV:c2463650-2463231 , Rv2199c SEQ ID NO:89 ATGCATATCGAAGCCCGACTGTTTGAGTTTGTCGCCGCGTTCTTCGTGGTGACGGCGGTGCTGT

ACGGCGTGTTGACCTCGATGTTCGCCACCGGTGGTGTCGAGTGGGCTGGCACCACTGCGCTGG CGCTTACCGGCGGCATGGCGTTGATCGTCGCCACCTTCTTCCGGTTTGTGGCCCGCCGGTTAG ATTCCCGGCCCGAGGACTACGAAGGCGCTGAAATCAGCGACGGCGCAGGAGAACTTGGATTCT TCAGTCCGCATAGCTGGTGGCCGATCATGGTCGCGTTGTCCGGCTCGGTGGCAGCGGTCGGCA TCGCGTTGTGGCTCCCGTGGCTGATCGCCGCCGGTGTGGCATTCATCCTCGCCTCGGCGGCCG

GATTGGTCTTCGAATATTACGTCGGTCCTGAGAAGCACTGA

>Rv2200c ctaC TB.seq 2463661 :2464749 MW:40449 >emb|AL123456|MTBH37RV:c2464749-2463658, ctaC SEQ ID NO:90 GTGACACCTCGCGGGCCAGGTCGTTTGCAACGCTTGTCGCAGTGCAGGCCTCAGCGCGGCTCC

GGAGGGCCTGCCCGTGGTCTTCGACAGCTGGCGCTCGCAGCAATGCTGGGGGCATTGGCCGT CACCGTCAGTGGATGCAGCTGGTCGGAAGCCCTGGGCATCGGTTGGCCGGAGGGCATTACCC CGGAGGCACACCTCAATCGAGAACTGTGGATCGGGGCGGTGATCGCCTCCCTGGCGGTTGGG GTAATCGTGTGGGGTCTCATCTTCTGGTCCGCGGTATTTCACCGGAAGAAGAACACCGACACTG AGTTGCCCCGCCAGTTCGGCTACAACATGCCGCTAGAGCTGGTTCTCACCGTCATACCGTTCCT

CATCATCTCGGTGCTGTTTTATTTCACCGTCGTGGTGCAGGAGAAGATGCTGCAGATAGCCAAG GATCCCGAGGTCGTGATTGATATCACGTCTTTCCAGTGGAATTGGAAGTTTGGCTATCAAAGGGT GAACTTCAAAGACGGCACACTGACCTATGATGGTGCCGATCCGGAGCGCAAGCGCGCCATGGT TTCCAAGCCAGAGGGCAAGGACAAGTACGGCGAAGAGCTGGTCGGGCCGGTGCGCGGGCTCA ACACCGAGGACCGGACCTACCTGAATTTCGACAAGGTCGAGACGTTGGGCACCAGCACCGAAA

TTCCGGTGCTGGTGCTGCCGTCCGGCAAGCGTATCGAATTCCAAATGGCCTCAGCCGATGTGAT ACACGCATTCTGGGTGCCGGAGTTCTTGTTCAAGCGTGACGTGATGCCTAACCCGGTGGCAAAC AACTCGGTCAACGTCTTCCAGATCGAAGAAATCACCAAGACCGGAGCATTCGTGGGCCACTGCG CCGAGATGTGTGGCACGTATCACTCGATGATGAACTTCGAGGTCCGCGTCGTGACCCCCAACG ATTTCAAGGCCTACCTGCAGCAACGCATCGACGGGAAGACAAACGCCGAGGCCCTGCGGGCGA

TCAACCAGCCGCCCCTTGCGGTGACCACCCACCCGTTTGATACTCGCCGCGGTGAATTGGCCC CGCAGCCCGTAGGTTAG >Rv2427c proA g-glutamyl phosphate reductase TB.seq 2724231 :2725475 MW:43746

>emb|AL123456|MTBH37RV:c2725475-2724228, proA SEQ ID NO:91

ATGACCGTGCCAGCACCGTCGCAGCTCGACTTGCGTCAAGAGGTGCACGACGCCGCACGCCG CGCCCGGGTGGCCGCCCGCCGGCTGGCATCGCTGCCGACGACTGTCAAAGACCGCGCGCTGC

ACGCGGCTGCCGACGAGCTACTGGCTCACCGCGACCAGATCCTGGCGGCCAACGCCGAAGAC CTGAACGCGGCGCGCGAGGCGGACACCCCGGCCGCCATGCTGGACCGGTTGTCCTTGAACCC GCAACGAGTCGACGGTATCGCCGCCGGGTTGCGGCAAGTCGCGGGACTGCGCGATCCGGTCG GTGAAGTGCTGCGTGGCTATACCCTGCCCAACGGGCTGCAGCTGCGCCAGCAGCGCGTCCCCC TGGGCGTGGTCGGCATGATCTACGAGGGCCGCCCCAATGTCACCGTGGATGCCTTCGGGCTGA

CACTCAAGTCGGGTAACGCTGCATTGCTGCGCGGCAGCTCGTCGGCCGCAAAGTCCAACGAGG CCCTGGTGGCGGTGTTACGCACCGCGCTGGTCGGCCTGGAGCTGCCGGCCGACGCGGTCCAG CTGCTGTCGGCTGCCGACCGCGCCACCGTCACTCACCTGATTCAGGCCCGCGGCCTGGTCGAT GTGGTGATTCCACGCGGGGGAGCGGGCCTGATCGAGGCGGTCGTACGCGATGCCCAGGTGCC CACCATCGAGACCGGCGTCGGGAACTGCCATGTCTACGTGCACCAAGCGGCCGACCTGGACGT

GGCCGAGCGTATCTTGCTGAACTCCAAGACGCGGCGGCCCAGCGTCTGCAACGCCGCCGAGA CGCTGCTGGTCGACGCAGCGATCGCCGAAACGGCGTTGCCTCGATTGCTGGCCGCCCTGCAGC ACGCCGGTGTCACCGTACATCTCGACCCGGACGAGGCCGACCTGCGCCGCGAATACCTGTCGC TGGACATCGCGGTGGCGGTGGTCGACGGTGTCGACGCTGCCATCGCCCATATCAACGAATACG GCACCGGGCACACAGAAGCGATTGTGACCACCAATCTTGATGCGGCCCAACGCTTTACCGAACA

GATCGATGCGGCCGCGGTGATGGTGAACGCATCAACGGCGTTCACCGACGGCGAGCAATTCGG CTTCGGCGCCGAGATCGGCATCTCCACCCAGAAACTGCATGCCCGCGGACCGATGGGACTACC GGAATTGACGTCGACCAAGTGGATCGCATGGGGAGCCGGCCACACCCGTCCGGCCTGA

>Rv2438c - similar to YHN4_YEAST P38795 TB.seq 2734793:2737006 MW:80492

>emb|AL123456|MTBH37RV:c2737006-2734790, Rv2438c SEQ ID NO:92

ATGGGACTGCTCGGCGGCCAATCAGGGCCCAGGGTCGGCAGCGGCCCAGTCGGTAGCATCCC CACGCCGGTCAATGCCGCCATCTGCCAGCAGCGCGGGGGATTCCACGGTGTCGAGCGTGGAT ACTCGGCGGGTGATTCGGGCGTTCTGACGTCGCTGGGCGACAATGAAAGGACGATGAACTTTT ACTCCGCCTACCAGCACGGGTTCGTGCGCGTTGCCGCCTGCACTCACCACACCACCATCGGTG

ACCCGGCGGCCAACGCCGCGTCGGTATTGGACATGGCCCGTGCGTGCCACGACGATGGCGCA GCGTTGGCGGTCTTTCCTGAGCTGACGCTGTCGGGCTACTCCATCGAGGACGTACTACTGCAG GACTCTCTGCTCGATGCCGTCGAGGACGCGCTGCTCGACCTGGTGACCGAATCCGCCGACCTG TTACCTGTACTGGTGGTCGGGGCTCCGCTGCGGCATCGACACCGCATCTACAACACCGCGGTC GTCATTCACCGCGGCGCCGTGCTCGGCGTGGTGCCCAAGTCGTATCTACCCACCTATCGCGAG

TTCTACGAGCGGCGCCAGATGGCGCCCGGAGACGGGGAGCGGGGCACGATCCGCATCGGTGG CGCCGACGTGGCCTTCGGCACGGACCTGTTGTTCGCCGCGTCAGATCTACCCGGCTTTGTGTT GCATGTGGAGATCTGCGAGGACATGTTTGTGCCGATGCCGCCCAGCGCCGAGGCGGCCCTGG CGGGCGCGACGGTGCTGGCGAATCTGTCCGGCAGCCCGATCACCATCGGCCGTGCCGAGGAC CGCCGGCTGCTTGCGCGCTCGGCGTCGGCGCGGTGTCTGGCTGCCTATGTCTATGCCGCCGC GGGGGAGGGGGAGTCAACGACGGACCTGGCCTGGGACGGTCAGACGATGATCTGGGAGAATG GCGCACTGCTCGCGGAGTCCGAACGTTTCCCCAAAGGAGTGCGCCGCAGTGTCGCCGACGTTG

ACACCGAGTTGCTTCGGTCGGAGCGGCTGCGGATGGGCACGTTCGACGACAACCGGCGTCAC CACCGGGAGTTAACGGAATCGTTCCGGCGCATCGACTTCGCACTCGACCCACCGGCAGGCGAC ATCGGACTGCTGCGCGAGGTCGAGCGGTTCCCGTTCGTTCCGGCCGATCCGCAACGATTGCAA CAGGATTGCTACGAGGCCTACAACATCCAGGTGTCTGGACTCGAGCAACGGTTGCGGGCGCTG GACTATCCGAAGGTCGTTATCGGTGTGTCCGGGGGATTGGACTCGACGCACGCGCTGATCGTC

GCGACCCATGCCATGGACCGCGAGGGCCGGCCGCGCAGCGACATTCTGGCGTTTGCGTTGCC CGGATTCGCCACCGGGGAGCACACTAAGAACAACGCGATCAAGCTGGCACGTGCGCTGGGGG TTACCTTCTCCGAAATCGATATCGGCGACACCGCTCGGTTGATGCTGCACACAATCGGCCATCC GTATTCGGTTGGCGAAAAAGTGTACGACGTCACCTTCGAGAACGTCCAGGCCGGGTTGCGCAC CGACTATCTTTTCCGTATCGCCAACCAGCGCGGGGGAATCGTACTGGGCACCGGGGACCTGTC

GGAGCTGGCACTGGGTTGGTCGACATACGGTGTCGGCGACCAGATGTCGCACTACAACGTCAA CGCCGGTGTGCCCAAGACGCTGATCCAGCACCTGATCCGGTGGGTCATTTCGGCGGGTGAGTT CGGTGAGAAGGTGGGTGAGGTATTGCAGTCGGTGCTCGACACCGAGATCACCCCCGAACTCAT TCCGACCGGCGAGGAGGAGCTGCAGAGCAGCGAGGCCAAGGTCGGACCTTTCGCCCTACAGG ACTTTTCGCTTTTTCAGGTACTGCGCTACGGATTTCGCCCGTCGAAGATTGCGTTTTTGGCCTGG

CATGCGTGGAACGATGCGGAGCGGGGCAACTGGCCGCCCGGCTTCCCAAAGAGCGAACGCCC GTCCTATTCATTGGCCGAAATCCGGCATTGGCTGCAGATTTTCGTCCAGCGGTTTTATTCGTtTA GCCAGTTCAAGCGTTCGGCATTGCCCAACGGCCCCAAGGTGTCCCACGGGGGCGCGTTGTCGC CGCGTGGGGATTGGCGGGCCCCGTCGGATATGTCAGCGCGAATCTGGCTCGATCAGATCGACC GTGAGGTGCCCAAGGGCTAG

>Rv2439c proB glutamate 5-kinase TB.seq 2737118:2738245 MW:38789 >emb|AL123456|MTBH37RV:c2738245-2737115, proB SEQ ID NO:93 ATGAGAAGTCCGCATCGGGACGCAATCCGGACCGCGCGCGGCCTTGTCGTGAAGGTCGGGAC CACGGCGCTTACCACACCGTCCGGGATGTTCGATGCCGGCCGGCTGGCCGGACTGGCCGAGG

CGGTCGAGCGGCGGATGAAGGCGGGTTCCGACGTCGTCATCGTGTCTTCGGGCGCCATCGCC GCCGGCATCGAGCCGCTCGGGCTGTCCCGTCGTCCCAAAGATCTGGCGACCAAGCAGGCGGC GGCCAGCGTCGGGCAGGTCGCGCTGGTGAACTCGTGGAGCGCGGCGTTCGCCCGCTACGGCC GCACGGTGGGCCAGGTGCTGCTGACCGCGCACGACATTTCGATGCGGGTGCAGCACACCAAC GCCCAACGCACGCTGGATCGGCTGCGCGCGTTGCACGCGGTGGCGATTGTCAACGAGAACGA

CACCGTGGCCACCAACGAGATCCGGTTCGGTGACAACGATCGGCTGTCTGCACTGGTGGCGCA CCTGGTCGGCGCCGACGCTTTGGTGCTGCTGTCGGACATCGACGGCCTCTACGACTGCGACCC GCGCAAAACCGCGGACGCGACGTTCATTCCGGAGGTGTCCGGGCCGGCGGATCTGGACGGTG TGGTCGCCGGCCGCAGTAGCCACCTGGGTACTGGCGGCATGGCGTCCAAGGTGGCGGCGGCG CTGTTGGCCGCCGACGCCGGGGTGCCGGTACTGCTGGCCCCCGCGGCCGACGCCGCGACCG CGCTCGCCGACGCGTCGGTGGGCACGGTGTTTGCGGCCCGGCCCGCGCGTCTGTCGGCCCGG CGGTTCTGGGTGCGTTATGCCGCCGAAGCAACCGGCGCACTGACTCTCGACGCCGGTGCGGTG

CGCGCTGTGGTGCGACAACGCCGGTCACTGCTGGCGGCGGGTATCACCGCGGTGTCCGGCCG GTTTTGCGGCGGCGATGTGGTCGAACTGCGTGCACCCGACGCGGCCATGGTAGCCCGCGGGG TGGTTGCCTACGACGCGTCCGAGCTGGCCACCATGGTGGGCCGGTCCACCTCTGAGCTACCCG GCGAGCTGCGCCGCCCGGTGGTGCACGCCGACGATCTGGTCGCGGTGTCGGCGAAGCAAGCT AAGCAAGTTTAG

>Rv2440c obg Obg GTP-binding protein TB.seq 2738248:2739684 MW:50430

>emb|AL123456|MTBH37RV:c2739684-2738245, obg SEQ ID NO:94 GTGCCTCGGTTTGTCGATCGGGTCGTCATCCACACCAGAGCGGGTTCGGGCGGTAACGGCTGC GCTTCGGTCCATCGCGAGAAATTCAAGCCGCTGGGCGGCCCCGATGGCGGAAATGGCGGCCG

GGGCGGCAGCATCGTCTTCGTCGTCGATCCGCAAGTGCACACCCTGCTCGACTTCCATTTCCGC CCGCATCTCACCGCGGCTTCGGGCAAGCACGGGATGGGCAATAACCGCGACGGGGCCGCCGG CGCGGATTTGGAAGTGAAAGTTCCCGAAGGCACCGTGGTATTGGACGAGAACGGCCGGCTACT GGCCGACCTGGTCGGCGCGGGCACCCGCTTTGAAGCCGCCGCCGGAGGCCGTGGCGGTTTGG GCAACGCCGCGCTGGCTTCCCGCGTGCGTAAGGCCCCCGGTTTCGCACTCCTCGGCGAAAAGG

GACAGTCCCGAGACCTCACCTTGGAACTCAAGACCGTCGCCGACGTCGGCCTGGTCGGGTTTC CGTCGGCCGGAAAATCCTCGCTGGTGTCGGCGATTTCGGCGGCCAAGCCGAAGATCGCCGACT ACCCGTTCACCACCCTGGTGCCCAACCTCGGTGTGGTCTCGGCTGGCGAGCACGCGTTCACCG TCGCCGACGTGCCGGGGTTGATCCCGGGCGCATCCCGGGGCCGTGGTCTGGGGCTGGACTTT CTGCGGCACATCGAGCGCTGCGCTGTACTGGTGCATGTGGTGGATTGCGCTACCGCCGAGCCG

GGCCGCGACCCCATCTCGGACATCGACGCGCTGGAAACGGAACTCGCGTGCTACACGCCCAC GCTGCAAGGGGACGCGGCTCTGGGCGATCTCGCCGCACGGCCGCGTGCGGTGGTCCTCAACA AAATCGATGTGCCGGAGGCCCGCGAGCTCGCGGAGTTCGTCCGTGACGACATCGCCCAGCGC GGCTGGCCGGTGTTCTGCGTGTCGACCGCAACCCGGGAAAACCTGCAGCCGTTGATCTTTGGG CTGTCGCAGATGATCTCGGACTACAACGCTGCGCGGCCGGTGGCGGTGCCACGGCGGCCGGT

GATTCGTCCGATTCCGGTGGACGACAGCGGTTTTACCGTCGAACCCGACGGGCATGGTGGCTT TGTCGTCAGCGGTGCCCGGCCCGAGCGTTGGATTGACCAGACCAACTTCGACAACGACGAGGC CGTCGGCTATCTCGCCGACCGGCTGGCGCGCCTGGGTGTCGAGGAGGAATTGCTGAGGCTGG GTGCGCGGTCAGGATGCGCGGTGACCATCGGCGAGATGACGTTCGATTGGGAGCCGCAAACG CCTGCGGGTGAGCCGGTCGCGATGTCCGGCCGGGGCACCGATCCGCGGCTGGACAGCAACAA

GCGGGTGGGCGCGGCCGAGCGAAAGGCCGCTCGGAGTCGGCGTCGCGAACACGGGGATGGC TGA >Rv2441c rpmA 50S ribosomal protein L27 TB.seq 2739773:2740030 MW:8969

>emb|AL123456|MTBH37RV:c2740030-2739770, rpmA SEQ ID NO:95

ATGGCACACAAGAAGGGGGCTTCCAGCTCGCGCAACGGTCGCGATTCCGCCGCCCAGCGGCT GGGGGTTAAGCGGTACGGCGGCCAGGTCGTCAAGGCCGGCGAGATCCTGGTCCGCCAGCGCG

GTACCAAATTCCATCCCGGCGTCAACGTCGGGCGTGGCGGCGATGACACCTTGTTCGCCAAGA CGGCCGGGGCGGTCGAGTTCGGCATCAAACGCGGACGTAAGACGGTGAGCATCGTCGGTTCG ACCACTGCCTGA

>Rv2442c rplU 50S ribosomal protein L21 TB.seq 2740048:2740359 MW:11152

>emb|AL123456|MTBH37RV:c2740359-2740045, rplU SEQ ID NO:96

ATGATGGCGACCTACGCAATCGTCAAGACCGGCGGCAAGCAGTACAAAGTCGCTGTCGGAGAT GTGGTCAAGGTCGAAAAGCTGGAATCCGAGCAGGGGGAGAAGGTGTCCCTGCCGGTGGCTCT GGTTGTCGACGGCGCCACCGTCACCACCGATGCGAAGGCACTGGCCAAGGTCGCGGTGACCG GTGAGGTGCTCGGGCACACCAAGGGCCCCAAGATCCGTATCCACAAGTTCAAGAACAAGACTG

GCTACCACAAACGGCAGGGACACCGTCAGCAGCTGACGGTCCTGAAGGTCACCGGCATCGCAT AA

>Rv2448c valS valyl-tRNA synthase TB.seq 2747596:2750223 MW:97822 >emb|AL123456|MTBH37RV:c2750223-2747593, valS SEQ ID NO:97

ATGCTGCCCAAGTCGTGGGATCCGGCCGCGATGGAGAGCGCCATCTATCAGAAGTGGCTGGAC GCTGGCTACTTCACCGCGGACCCGACCAGCACCAAGCCGGCCTATTCGATCGTGCTGCCGCCG CCGAACGTGACCGGCAGCCTGCACATGGGCCACGCGCTGGAACACACCATGATGGACGCCTTG ACGCGGCGCAAGCGGATGCAGGGCTATGAGGTGCTCTGGCAGCCGGGCACCGACCATGCCGG GATCGCCACCCAGAGCGTGGTCGAGCAGCAGCTGGCGGTCGACGGCAAGACTAAAGAAGACCT

CGGCCGCGAGCTGTTCGTGGACAAGGTGTGGGATTGGAAGCGAGAGTCTGGCGGTGCCATCG GCGGCCAGATGCGCCGACTCGGTGACGGGGTGGACTGGAGCCGCGACCGGTTCACCATGGAC GAAGGTCTGTCGCGGGCGGTGCGCACGATCTTCAAGCGGCTTTATGACGCCGGGCTGATCTAT CGGGCCGAGCGGCTGGTCAACTGGTCGCCGGTGCTGCAGACCGCGATCTCCGACCTCGAGGT CAACTACCGCGACGTCGAAGGCGAGCTGGTGTCGTTTAGGTACGGCTCGCTTGACGACTCGCA

ACCCCACATCGTGGTCGCCACCACCCGGGTCGAGACGATGCTGGGCGATACCGCGATCGCCGT CCATCCCGATGACGAGCGCTACCGTCACCTGGTCGGCACCAGCCTGGCGCACCCATTCGTCGA CCGGGAGCTGGCCATTGTCGCCGACGAGCACGTGGACCCTGAATTCGGCACCGGCGCGGTCA AAGTCACACCCGCCCACGACCCCAACGACTTCGAAATCGGGGTGCGCCACCAGCTGCCGATGC CCTCGATCCTGGACACCAAGGGCCGGATCGTCGACACCGGAACGCGATTCGACGGCATGGACC

GCTTCGAGGCACGGGTCGCGGTGCGCCAAGCGCTCGCGGCCCAGGGCCGCGTGGTCGAAGAA AAGCGACCCTACCTGCACAGCGTCGGACACTCCGAACGCAGCGGCGAGCCGATCGAGCCGCG GCTATCCCTGCAGTGGTGGGTCCGGGTGGAATCGCTGGCCAAAGCGGCCGGGGATGCGGTGC GCAACGGGGACACCGTGATTCACCCGGCCAGCATGGAACCCCGCTGGTTCTCCTGGGTCGACG ACATGCACGACTGGTGCATCTCGCGACAGCTCTGGTGGGGGCATCGGATCCCGATCTGGTACG GACCCGACGGCGAACAGGTGTGCGTCGGCCCGGACGAAACACCCCCGCAGGGCTGGGAACAG GATCCTGACGTGCTGGATACCTGGTTTTCGTCGGCGCTGTGGCCGTTTTCCACGCTGGGTTGGC

CGGACAAGACGGCGGAGCTGGAAAAGTTCTATCCGACAAGCGTTCTGGTTACCGGCTATGACAT CTTGTTCTTTTGGGTGGCCAGAATGATGATGTTCGGCACCTTCGTCGGCGACGACGCCGCCATC ACCCTCGACGGCCGCCGGGGCCCGCAGGTGCCGTTCACCGACGTGTTTCTGCATGGGCTGATC CGCGACGAGTCTGGCCGCAAGATGAGCAAGTCCAAGGGCAACGTCATCGACCCGCTGGATTGG GTGGAAATGTTCGGGGCCGATGCGCTGCGGTTCACGCTGGCCCGCGGGGCCAGTCCCGGTGG

TGACTTGGCGGTGAGCGAGGATGCCGTGCGGGCGTCGCGCAATTTCGGGACCAAGCTGTTCAA CGCCACTCGGTACGCACTGCTCAATGGCGCCGCGCCAGCACCCCTGCCATCGCCGAACGAGCT GACCGACGCCGACCGCTGGATTCTCGGAAGGTTGGAAGAGGTTCGGGCCGAAGTTGATTCGGC CTTCGACGGATACGAGTTCAGCCGCGCTTGTGAGTCCCTGTATCACTTCGCCTGGGACGAATTC TGCGACTGGTACCTCGAACTGGCCAAAACGCAGCTTGCCCAGGGACTCACACACACCACCGCC

GTGCTGGCCGCCGGGCTGGACACGCTGCTGCGCCTGCTGCACCCGGTGATTCCCTTCCTCACC GAGGCGCTATGGCTGGCGCTGACCGGCAGGGAATCGCTGGTCAGCGCCGACTGGCCGGAGCC TTCCGGGATTAGCGTGGACCTTGTTGCCGCGCAACGGATTAACGATATGCAGAAGTTGGTGACC GAAGTGCGGCGGTTCCGCAGCGATCAAGGTCTGGCCGACCGGCAGAAGGTTCCGGCCCGAAT GCACGGTGTGCGGGACTCGGATCTGAGCAACCAGGTGGCCGCCGTGACCTCGCTGGCGTGGC

TCACCGAGCCGGGCCCGGATTTTGAGCCGTCGGTCTCGTTGGAGGTTCGGCTCGGCCCCGAGA TGAACCGCACCGTCGTCGTCGAGCTCGACACCTCGGGCACCATCGACGTGGCCGCCGAGCGT CGCCGCCTGGAAAAGGAGTTGGCCGGCGCCCAAAAGGAGCTGGCGTCGACCGCCGCCAAGTT GGCCAACGCGGACTTTCTGGCCAAAGCGCCCGACGCCGTCATTGCCAAGATCCGGGACCGCCA GCGCGTGGCGCAGCAGGAAACCGAGCGCATCACCACCCGGTTGGCTGCGCTGCAATGA

>Rv2482c plsB2 TB.seq 2786915:2789281 MW:88284 >emb|AL123456|MTBH37RV:c2789281- 2786912, plsB2 SEQ ID NO:98 GTGACCAAACCGGCGGCCGATGCCAGCGCGGTGCTTACTGCCGAGGACACACTGGTGCTGGC TTCCACGGCGACGCCGGTCGAGATGGAGCTGATCATGGGCTGGCTGGGCCAGCAGCGTGCAC

GCCATCCGGACTCGAAGTTCGACATATTGAAGCTGCCACCGCGCAACGCTCCGCCGGCGGCGC TGACGGCACTGGTCGAGCAGCTCGAGCCCGGCTTCGCATCCAGCCCGCAATCTGGCGAGGAC CGTTCTATCGTGCCGGTTCGGGTGATCTGGCTGCCTCCCGCCGATCGCAGCCGGGCGGGCAAG GTGGCCGCACTGCTCCCGGGTCGGGATCCCTACCATCCCAGCCAGCGTCAGCAGCGTCGCATC CTGCGTACCGATCCCAGGCGCGCGCGGGTGGTGGCCGGCGAGTCGGCCAAGGTGTCCGAACT

GCGCCAGCAGTGGCGCGATACCACGGTGGCAGAGCACAAGCGCGATTTCGCCCAGTTCGTCAG CCGCCGAGCGCTGTTGGCGCTGGCGCGCGCCGAATATCGGATCCTTGGACCGCAATACAAATC TCCCCGGCTGGTGAAGCCGGAGATGTTGGCGTCCGCACGATTTCGTGCCGGCCTGGACCGGAT TCCGGGCGCCACGGTCGAAGATGCCGGGAAGATGCTCGACGAACTCTCCACCGGATGGAGCC AGGTGTCGGTAGACCTGGTTTCCGTCCTCGGCAGGCTGGCTAGCCGCGGCTTCGATCCGGAAT TCGACTACGACGAGTATCAGGTCGCGGCGATGCGCGCCGCACTGGAGGCTCATCCGGCGGTC CTGCTGTTCTCGCACCGGTCCTACATCGACGGCGTGGTGGTACCGGTGGCCATGCAGGACAAC

CGGTTACCGCCGGTGCACATGTTCGGCGGCATCAACCTGTCGTTCGGTCTCATGGGACCCCTC ATGCGGCGCTCGGGGATGATCTTCATCCGGCGCAATATCGGCAACGACCCACTGTATAAGTACG TGCTCAAGGAGTACGTGGGCTACGTGGTCGAGAAGCGGTTCAACCTGAGCTGGTCCATCGAAG GCACCCGGTCGCGCACCGGAAAGATGTTGCCGCCCAAGCTCGGTTTGATGAGCTACGTGGCCG ATGCTTACCTGGACGGCCGCAGTGACGACATCCTGCTGCAGGGGGTTTCGATTTGCTTCGATCA

GCTGCACGAGATCACCGAATACGCCGCCTACGCGCGTGGCGCGGAGAAGACGCCCGAAGGTT TGCGCTGGCTCTACAACTTCATCAAGGCGCAGGGGGAACGCAACTTCGGCAAGATCTACGTTCG CTTCCCCGAAGCGGTCTCGATGCGCCAGTACCTCGGCGCACCGCACGGCGAGCTGACCCAGG ATCCGGCCGCGAAACGGCTTGCGTTGCAGAAGATGTCGTTCGAGGTGGCCTGGAGGATTTTGC AGGCGACGCCGGTGACCGCGACGGGTTTGGTGTCCGCACTGCTGCTCACCACCCGCGGCACC

GCGTTGACGCTCGACCAGCTGCACCACACGTTGCAGGACTCACTGGACTATCTGGAACGCAAA CAATCGCCGGTTTCGACAAGCGCATTGCGACTGCGCTCGCGCGAAGGCGTCCGTGCGGCGGC GGACGCGTTGTCCAACGGCCACCCGGTCACTCGGGTCGACAGTGGCCGGGAGCCGGTATGGT ACATAGCGCCTGACGACGAGCACGCCGCGGCGTTCTACCGGAACTCGGTGATCCATGCGTTTTT GGAGACCTCGATCGTCGAGCTCGCGCTGGCCCATGCCAAGCACGCCGAAGGTGACCGCGTCG

CCGCGTTCTGGGCCCAGGCGATGCGGTTGCGGGATCTGCTGAAGTTCGACTTCTATTTCGCGG ATTCCACGGCGTTTCGGGCCAACATCGCCCAAGAGATGGCCTGGCACCAAGACTGGGAGGATC ATCTTGGCGTCGGGGGCAATGAGATCGACGCGATGCTGTATGCCAAACGGCCGCTGATGTCGG ACGCGATGTTGCGGGTCTTCTTCGAAGCCTATGAGATCGTTGCCGACGTGTTGCGCGATGCTCC GCCTGACATCGGTCCTGAGGAGTTGACGGAGCTGGCGCTCGGCCTCGGCCGTCAGTTTGTGGC

ACAGGGCCGGGTCCGCAGCAGCGAACCGGTATCGACGCTGCTGTTCGCCACTGCACGCCAGG TCGCCGTCGATCAGGAGCTGATAGCGCCGGCGGCCGACCTCGCCGAACGTAGGGTCGCCTTC CGGCGGGAGTTACGAAACATTCTGCGGGATTTCGACTATGTCGAGCAGATCGCGCGCAACCAG TTCGTCGCCTGCGAGTTCAAAGCGCGTCAAGGACGCGACCGAATCTAA

>Rv2509 - putative oxidoreductase TB.seq 2824676:2825479 MW:28014 >emb|AL123456|MTBH37RV:2824676-2825482, Rv2509 SEQ ID NO:99

ATGCCGATACCCGCGCCCAGCCCCGACGCACGTGCCGTTGTCACCGGGGCTTCGCAGAACATC GGCGCGGCGCTGGCCACCGAACTGGCCGCACGCGGGCACCACCTGATCGTCACCGCACGACG CGAGGACGTGTTGACCGAGTTGGCTGCCCGGCTGGCCGACAAGTACCGCGTCACGGTCGACG

TGCGACCGGCCGATCTGGCCGATCCGCAAGAACGATCGAAACTGGCCGACGAGCTGGCTGCC CGGCCCATCTCGATCCTGTGCGCCAACGCGGGTACCGCGACATTCGGCCCGATCGCATCGCTC GATCTTGCCGGCGAAAAGACGCAGGTGCAGTTGAATGCCGTGGCGGTGCACGACCTTACGTTG GCGGTGTTGCCGGGCATGATCGAGCGCAAGGCCGGCGGCATCTTGATTTCTGGTTCGGCGGCC GGCAATTCACCGATTCCCTACAACGCCACCTATGCCGCGACCAAGGCCTTCGTGAACACCTTCA GCGAATCTCTGCGCGGTGAGCTACGCGGCTCCGGCGTGCACGTCACGGTGCTGGCCCCGGGC CCGGTTCGCACCGAGCTACCGGATGCCTCCGAAGCGTCACTGGTCGAGAAGCTGGTGCCGGAC

TTCCTGTGGATCTCGACGGAGCACACCGCCCGGGTATCGCTGAATGCCTTGGAGCGCAACAAG ATGCGCGTCGTTCCGGGTCTGACGTCAAAGGCGATGTCGGTGGCCAGCCAATACGCTCCGCGC GCCATCGTGGCGCCAATCGTGGGTGCCTTTTACAAGAGGCTTGGGGGCAGCTAG

>Rv2524c fas fatty acid synthase TB.seq 2840124:2849330 MW:326226

>emb|AL123456|MTBH37RV:c2849330-2840121 , fas SEQ ID NO:100

GTGACGATCCACGAGCACGACCGGGTGTCCGCTGATCGCGGCGGGGACAGCCCGCATACCAC CCACGCTCTGGTCGATCGCCTCATGGCTGGTGAGCCCTACGCTGTCGCATTCGGTGGCCAGGG CAGCGCCTGGCTGGAAACCCTCGAAGAGCTGGTGTCGGCCACCGGGATAGAAACCGAGTTGGC GACGTTGGTCGGTGAGGCAGAGCTGTTGCTCGATCCGGTCACCGACGAGCTGATTGTGGTGCG

CCCGATCGGTTTCGAGCCGCTGCAATGGGTACGCGCACTGGCGGCCGAGGACCCGGTTCCGT CCGACAAGCACCTGACGTCGGCCGCCGTGTCGGTGCCCGGCGTGTTGCTTACCCAGATCGCGG CGACCCGGGCGCTGGCCCGTCAAGGCATGGACCTCGTGGCCACCCCGCCGGTCGCCATGGCG GGGCATTCGCAAGGTGTGCTGGCGGTGGAAGCCCTCAAGGCTGGTGGGGCACGCGACGTCGA GCTGTTTGCCTTGGCCCAGTTGATCGGTGCCGCCGGAACGCTGGTGGCCCGCCGGCGCGGAA

TTTCCGTCCTGGGCGATCGCCCGCCGATGGTATCGGTCACCAACGCCGACCCCGAGCGCATCG GCCGGTTGCTCGACGAGTTCGCCCAGGACGTGCGCACGGTGCTGCCACCGGTGTTGTCCATCC GCAACGGCCGGCGTGCCGTCGTCATCACCGGCACCCCCGAGCAGCTGTCGCGTTTCGAGCTTT ATTGCCGCCAGATCTCCGAGAAGGAAGAAGCCGACCGCAAGAACAAGGTCCGCGGCGGCGAC GTCTTCTCGCCGGTCTTCGAGCCGGTGCAGGTGGAGGTGGGCTTTCACACCCCGCGGCTATCC

GACGGGATCGACATCGTCGCGGGCTGGGCCGAGAAGGCGGGCCTCGATGTCGCCTTGGCTCG GGAGCTGGCCGATGCCATCTTGATCAGAAAGGTCGACTGGGTCGACGAGATCACCCGTGTCCA CGCGGCCGGCGCCCGCTGGATCCTCGACCTGGGGCCGGGCGACATCCTGACCCGACTGACCG CACCGGTGATCCGCGGCCTGGGCATCGGCATCGTGCCGGCGGCTACCCGCGGTGGCCAGCGC AACCTGTTCACCGTCGGCGCCACCCCCGAGGTTGCCCGGGCCTGGTCGAGCTACGCACCGACC

GTGGTTCGCCTCCCCGACGGCAGGGTCAAGCTCTCGACGAAGTTCACCCGGCTGACCGGCCGC TCGCCGATCCTGCTCGCGGGCATGACCCCGACCACCGTGGACGCCAAGATCGTCGCCGCGGC GGCCAACGCCGGGCACTGGGCCGAGCTGGCCGGCGGCGGGCAGGTCACCGAAGAGATCTTC GGTAACCGCATCGAACAAATGGCCGGCCTGCTCGAGCCGGGCCGCACCTATCAGTTCAACGCG CTGTTCCTCGATCCCTACCTGTGGAAGCTTCAGGTGGGCGGCAAGCGGTTGGTGCAGAAGGCC

CGCCAGTCCGGCGCCGCGATCGACGGCGTGGTGATCAGCGCCGGCATCCCAGACCTCGACGA GGCCGTCGAGCTGATCGACGAACTGGGCGACATCGGCATCAGCCACGTCGTGTTCAAACCCGG GACCATCGAGCAGATCCGCTCGGTGATTCGCATCGCCACCGAGGTGCCCACCAAGCCGGTGAT CATGCACGTCGAGGGCGGGCGCGCCGGCGGGCACCATTCCTGGGAGGATCTCGACGACCTGC TGCTGGCTACCTACTCGGAGTTGCGCTCACGCGCCAACATCACGGTGTGCGTCGGCGGCGGCA TTGGCACCCCGAGAAGGGCTGCGGAATATTTGTCCGGGCGCTGGGCGCAGGCCTACGGCTTCC CATTGATGCCGATCGACGGCATCCTGGTCGGCACCGCGGCGATGGCCACCAAGGAATCCACCA

CGTCGCCATCGGTCAAGCGGATGCTCGTCGACACTCAGGGCACCGACCAATGGATCAGCGCCG GAAAAGCGCAGGGCGGCATGGCCTCCAGCCGCAGTCAGCTCGGTGCCGATATCCACGAGATC GACAACAGCGCATCCCGGTGCGGGCGGCTGCTCGACGAGGTGGCCGGTGACGCGGAGGCGG TCGCGGAGCGTCGCGACGAGATCATCGCGGCGATGGCCAAGACCGCCAAGCCCTACTTCGGC GACGTCGCCGACATGACCTACCTGCAGTGGCTGCGGCGCTACGTCGAACTGGCCATCGGGGAA

GGCAACTCGACCGCCGACACCGCCTCGGTGGGCAGCCCGTGGCTGGCCGACACCTGGCGGGA CCGCTTCGAGCAGATGCTGCAGCGTGCCGAAGCCCGGTTGCACCCACAGGATTTCGGCCCGAT CCAGACGCTATTCACCGATGCTGGCCTGCTGGACAATCCGCAGCAGGCGATCGCCGCCCTGCT GGCGCGCTACCCCGACGCCGAGACCGTGCAGTTGCATCCCGCGGATGTGCCCTTTTTCGTGAC GTTGTGCAAGACGCTGGGCAAGCCGGTCAACTTCGTGCCGGTGATCGACCAGGACGTGCGGC

GCTGGTGGCGCAGCGACTCGCTGTGGCAGGCCCACGACGCCCGCTACGACGCCGATGCGGTG TGCATCATTCCGGGCACCGCGTCGGTAGCCGGCATCACCCGGATGGATGAACCCGTCGGTGAG TTGCTGGACCGTTTCGAGCAAGCCGCAATCGATGAAGTGCTCGGCGCCGGTGTCGAGCCGAAG GATGTCGCGTCGCGCCGGCTGGGCCGCGCCGACGTGGCCGGACCGTTGGCTGTCGTCCTCGA CGCACCCGATGTGCGCTGGGCCGGTCGCACCGTGACCAACCCGGTGCATCGGATCGCCGACC

CGGCCGAATGGCAGGTGCACGATGGACCCGAAAACCCGCGCGCCACACACTCATCCACCGGC GCCCGGCTGCAGACGCACGGCGACGACGTCGCCTTGAGCGTGCCCGTCTCGGGCACCTGGGT CGACATCCGATTCACGTTGCCGGCCAACACCGTCGATGGCGGCACCCCGGTGATCGCCACCGA GGACGCCACCAGCGCCATGCGCACGGTGCTGGCGATCGCCGCCGGTGTCGACAGCCCGGAGT TCTTGCCTGCGGTGGCCAACGGGACGGCCACTTTGACGGTGGACTGGCACCCCGAGCGTGTTG

CCGACCACACCGGCGTCACCGCCACGTTCGGTGAGCCGCTGGCACCCAGCCTCACCAACGTG CCCGACGCGCTCGTCGGCCCTTGTTGGCCAGCGGTTTTCGCGGCCATCGGATCGGCGGTCACC GACACCGGTGAGCCGGTGGTGGAAGGCCTGCTGAGCCTGGTGCATCTGGACCACGCCGCCCG CGTGGTCGGTCAGCTGCCCACGGTCCCGGCCCAATTGACCGTCACCGCAACGGCTGCCAACGC AACCGATACGGACATGGGCCGCGTCGTGCCGGTCTCGGTCGTCGTTACCGGCGCCGATGGCG

CCGTGATCGCCACTCTCGAGGAGCGATTCGCGATCCTGGGTCGCACCGGTTCCGCCGAGCTCG CCGACCCGGCGCGAGCCGGTGGCGCGGTGTCGGCGAACGCCACCGACACCCCGCGCCGTCG CCGCCGCGACGTCACGATCACCGCGCCGGTCGACATGCGCCCGTTCGCGGTGGTGTCCGGCG ACCACAACCCCATTCACACCGACCGGGCCGCCGCGCTGCTTGCCGGCCTGGAGTCGCCGATC GTGCACGGCATGTGGCTGTCGGCCGCGGCGCAACACGCGGTGACCGCCACCGACGGGCAGG

CCCGGCCACCGGCCCGGCTGGTCGGCTGGACCGCGCGGTTTTTGGGCATGGTGCGCCCCGGC GACGAGGTGGACTTCCGCGTCGAGCGCGTCGGAATCGACCAGGGCGCAGAGATTGTGGACGT GGCCGCGCGCGTCGGGTCGGATCTAGTGATGTCGGCCTCCGCGCGACTGGCCGCACCCAAGA CGGTCTACGCATTCCCCGGCCAGGGCATCCAACACAAGGGCATGGGCATGGAGGTGCGCGCC CGCTCCAAGGCGGCCCGCAAGGTGTGGGACACCGCGGACAAGTTCACCCGCGACACCCTGGG CTTCTCGGTACTGCACGTGGTCCGCGACAACCCGACCAGCATCATCGCCAGCGGTGTGCACTA CCACCACCCCGACGGGGTGCTCTACCTGACGCAGTTCACCCAGGTCGCGATGGCGACGGTGG

CGGCCGCGCAGGTCGCCGAGATGCGTGAACAGGGAGCCTTCGTCGAAGGCGCCATCGCGTGC GGCCACTCGGTCGGCGAGTACACCGCGCTGGCCTGCGTGACCGGCATCTACCAACTGGAAGC CTTGCTGGAGATGGTGTTTCACCGCGGGTCGAAGATGCACGACATCGTTCCGCGCGACGAGCT CGGCCGCTCCAACTATCGGCTGGCGGCCATCCGGCCGTCCCAGATCGACCTCGACGACGCCG ACGTGCCCGCGTTCGTCGCCGGGATCGCGGAGAGCACCGGTGAATTCCTGGAGATCGTGAATT

TCAACCTGCGTGGCTCGCAATACGCGATCGCGGGCACGGTACGCGGCCTCGAGGCGCTCGAG GCCGAGGTGGAGCGGCGCCGCGAGCTCACCGGCGGCCGACGGTCGTTCATTTTGGTGCCCGG CATCGATGTTCCGTTCCACTCGCGAGTGCTGCGGGTCGGGGTGGCCGAATTCCGGCGCTCGCT GGACCGGGTCATGCCGCGCGACGCGGACCCCGACCTGATCATCGGGCGCTACATTCCCAACCT GGTGCCGCGGTTGTTCACCCTGGACCGCGACTTCATCCAGGAAATCCGGGATTTGGTGCCCGC

CGAGCCGCTCGACGAGATCCTCGCCGACTACGACACCTGGCTTCGCGAGCGTCCGCGCGAGAT GGCGCGCACGGTGTTCATCGAGCTGCTGGCATGGCAATTCGCCAGCCCGGTGCGCTGGATCGA GACGCAGGATCTGCTGTTCATCGAGGAGGCCGCCGGCGGGCTGGGTGTGGAGCGATTCGTCG AGATCGGTGTGAAGAGCTCACCGACGGTGGCGGGTCTTGCCACCAACACCCTCAAACTGCCCG AATACGCCCACAGCACAGTGGAAGTGCTCAACGCCGAGCGTGATGCCGCGGTGCTGTTCGCCA

CCGACACCGACCCGGAGCCGGAGCCGGAGGAAGACGAGCCGGTCGCGGAATCGCCCGCGCC GGACGTCGTCTCGGAAGCCGCCCCCGTCGCGCCGGCCGCTTCGTCGGCGGGCCCGCGTCCCG ACGATCTGGTTTTCGACGCCGCCGATGCCACGCTGGCGCTGATCGCGCTCTCGGCCAAGATGC GCATCGACCAGATCGAAGAACTCGACTCCATCGAGTCCATCACCGACGGTGCGTCGTCGCGGC GCAACCAGCTGCTGGTGGACCTGGGCTCCGAGCTGAACCTCGGTGCCATTGACGGCGCCGCC

GAATCGGACCTGGCCGGTCTGCGCTCACAGGTGACCAAACTGGCGCGCACCTACAAGCCTTAC GGCCCAGTGCTTTCCGACGCCATCAACGACCAGCTTCGCACCGTCCTCGGACCGTCGGGCAAG CGGCCCGGCGCCATCGCCGAGCGGGTGAAGAAGACCTGGGAGCTCGGTGAGGGCTGGGCCA AGCATGTCACCGTCGAGGTCGCGCTGGGCACCCGCGAGGGCAGCAGCGTTCGCGGCGGCGCC ATGGGCCACCTGCACGAGGGCGCGCTGGCCGATGCCGCCTCCGTCGACAAGGTCATCGACGC

GGCGGTCGCATCGGTGGCCGCGCGCCAGGGCGTTTCGGTAGCGCTGCCGTCGGCCGGTAGTG GTGGCGGCGCCACCATCGACGCGGCCGCGCTCAGCGAGTTCACCGACCAAATCACCGGCCGT GAGGGCGTGCTGGCCTCCGCGGCCCGCCTGGTGCTGGGGCAGCTGGGACTGGACGACCCCGT CAACGCCTTGCCGGCCGCCCCCGATTCCGAGCTGATCGACTTGGTCACCGCCGAACTGGGAGC GGACTGGCCGCGGTTGGTGGCACCGGTGTTCGACCCCAAGAAGGCCGTCGTATTCGACGACC

GCTGGGCCAGCGCCCGCGAGGACCTGGTGAAGCTGTGGCTGACCGACGAGGGCGACATCGAC GCCGACTGGCCGCGCCTGGCGGAGCGCTTCGAGGGTGCCGGCCACGTCGTGGCGACCCAGG CTACCTGGTGGCAAGGTAAGTCGCTGGCCGCGGGCCGGCAGATCCATGCATCGCTGTACGGCC GCATCGCCGCCGGCGCCGAGAACCCCGAACCCGGCCGCTACGGCGGCGAAGTTGCCGTGGTG ACCGGCGCTTCGAAGGGTTCGATCGCCGCGTCGGTGGTGGCTCGGCTGCTCGACGGCGGAGC CACCGTCATCGCGACCACCTCCAAGCTCGACGAGGAGCGGCTGGCGTTCTACCGCACGCTGTA TCGCGACCACGCCCGTTACGGCGCGGCGCTGTGGCTGGTCGCGGCGAACATGGCGTCCTACT

CCGACGTCGACGCCCTGGTCGAATGGATCGGCACCGAACAGACCGAAAGCCTTGGGCCGCAGT CGATTCACATCAAAGACGCGCAGACCCCGACGCTGCTGTTCCCGTTCGCGGCGCCACGCGTGG TCGGGGACCTGTCGGAGGCCGGTTCGCGCGCCGAGATGGAGATGAAAGTGCTGCTGTGGGCC GTGCAACGGCTGATCGGCGGCCTGTCGACGATCGGCGCCGAACGCGACATCGCGTCGCGGCT GCACGTGGTGCTGCCCGGCTCGCCCAACCGTGGCATGTTCGGCGGCGACGGCGCCTACGGCG

AAGCCAAGTCCGCGCTGGATGCCGTGGTGAGCCGCTGGCACGCCGAGTCGTCCTGGGCGGCA CGGGTCAGCCTGGCGCACGCGCTCATCGGCTGGACCCGCGGCACCGGGCTGATGGGCCACAA CGATGCCATCGTGGCCGCCGTCGAAGAGGCCGGGGTCACCACCTACTCGACCGACGAGATGG CGGCGCTGCTGCTCGACCTGTGTGATGCGGAATCCAAGGTGGCTGCGGCGCGTTCGCCGATCA AGGCCGACCTGACCGGGGGCCTGGCCGAGGCCAACCTCGACATGGCCGAGCTGGCGGCCAAG

GCGCGCGAGCAGATGTCGGCAGCGGCGGCCGTCGACGAGGACGCCGAGGCCCCTGGCGCCA TCGCCGCGCTGCCGTCGCCGCCCCGGGGTTTCACCCCCGCACCGCCGCCGCAATGGGACGAC CTCGATGTCGACCCGGCCGACCTGGTGGTGATCGTCGGCGGCGCCGAAATCGGCCCGTACGG CTCGTCACGCACCCGGTTCGAGATGGAGGTCGAAAACGAGCTGTCGGCGGCCGGCGTGCTGG AGCTGGCCTGGACCACTGGGTTGATCCGCTGGGAGGACGACCCGCAACCCGGTTGGTACGACA

CCGAATCCGGCGAAATGGTCGACGAATCCGAGTTGGTGCAGCGCTACCACGACGCCGTGGTGC AGCGCGTCGGCATTCGCGAATTCGTTGATGACGGCGCGATCGACCCCGACCACGCCTCGCCGC TGCTGGTGTCGGTGTTCCTGGAGAAGGACTTCGCGTTCGTGGTGTCCTCGGAGGCCGATGCGC GCGCCTTCGTCGAGTTCGATCCCGAGCACACGGTCATCCGGCCGGTGCCCGACTCCACCGACT GGCAGGTCATCCGCAAGGCCGGCACCGAGATCCGGGTGCCGCGAAAGACCAAGCTGTCCCGC

GTCGTCGGCGGCCAGATCCCGACCGGGTTCGACCCGACGGTGTGGGGCATCAGCGCAGACAT GGCCGGTTCCATCGACCGGTTGGCGGTATGGAACATGGTGGCGACCGTCGACGCGTTCCTGTC GTCCGGTTTCAGCCCGGCCGAGGTGATGCGTTACGTGCACCCGAGTTTGGTGGCCAACACCCA GGGCACCGGCATGGGCGGCGGCACGTCGATGCAGACGATGTACCACGGCAATCTGTTGGGCC GCAACAAGCCGAACGACATCTTCCAGGAAGTCTTGCCGAATATCATTGCCGCGCACGTGGTTCA

GTCCTACGTCGGTAGCTACGGTGCGATGATCCACCCGGTAGCCGCGTGCGCCACCGCCGCGGT GTCGGTCGAGGAAGGTGTCGACAAGATCCGGTTGGGCAAGGCTCAACTGGTGGTGGCCGGCG GCCTGGATGACCTGACGCTGGAGGGCATCATCGGATTCGGTGACATGGCCGCCACCGCCGACA CGTCCATGATGTGCGGCCGCGGCATCCACGACTCGAAGTTTTCCCGGCCCAACGACCGCCGCC GTCTGGGCTTCGTCGAAGCCCAAGGCGGCGGGACGATCCTGTTGGCCCGCGGGGACCTGGCG

CTGCGGATGGGGCTGCCGGTGCTGGCGGTGGTGGCGTTCGCGCAGTCGTTCGGCGACGGCGT GCACACCTCGATCCCGGCCCCGGGCCTGGGCGCGCTGGGGGCGGGCCGCGGCGGCAAGGAT TCACCGCTGGCGCGGGCGCTGGCCAAGCTGGGCGTGGCCGCCGACGACGTGGCGGTCATCTC CAAGCACGACACCTCGACGCTGGCCAACGATCCCAACGAGACCGAGTTGCATGAACGGCTCGC CGACGCCCTGGGCCGTTCCGAGGGCGCCCCGCTGTTCGTGGTGTCGCAGAAGAGCCTGACCG GCCACGCCAAGGGCGGCGCGGCGGTCTTCCAGATGATGGGGCTCTGCCAGATATTGCGGGAT GGGGTGATCCCACCCAACCGCAGCCTCGACTGCGTCGACGACGAGCTGGCCGGCTCCGCGCA

TTTCGTGTGGGTGCGTGACACGTTGCGGCTCGGCGGCAAGTTCCCACTCAAGGCCGGCATGCT GACCAGCCTCGGGTTCGGCCATGTGTCGGGCCTGGTCGCGTTGGTGCATCCGCAGGCGTTCAT CGCCTCGCTGGATCCCGCACAGCGCGCGGACTACCAGCGGCGTGCCGACGCCCGCCTGCTGG CCGGTCAGCGCCGGCTGGCCTCGGCGATTGCCGGTGGTGCGCCGATGTACCAGCGGCCCGGT GACCGTCGCTTCGACCACCACGCGCCCGAGCGGCCGCAGGAGGCGTCGATGCTGCTGAATCC

GGCGGCCCGGCTGGGTGACGGCGAGGCGTATATCGGCTGA

>Rv2555c alaS alanyl-tRNA synthase TB.seq 2873772:2876483 MW:97326 >emb|AL123456|MTBH37RV:c2876483-2873769, alaS SEQ ID NO: 101 GTGCAGACACACGAGATCAGGAAGCGGTTCCTCGATCATTTCGTGAAGGCGGGCCACACCGAG

GTGCCCAGCGCCTCGGTGATCCTCGACGACCCCAACCTGTTGTTCGTCAACGCCGGGATGGTC CAGTTCGTGCCTTTCTTCTTGGGACAGCGCACGCCGCCGTACCCGACGGCCACCAGCATCCAG AAGTGCATCCGTACCCCCGATATCGACGAGGTGGGCATAACCACCCGGCACAACACGTTTTTTC AGATGGCCGGCAATTTCAGCTTCGGCGACTATTTCAAACGCGGGGCCATTGAACTGGCCTGGG CACTGCTGACCAACAGCCTCGCCGCCGGCGGCTACGGCCTGGACCCGGAAAGAATCTGGACG

ACAGTCTATTTCGACGACGACGAAGCTGTCCGGCTATGGCAGGAGGTTGCCGGGCTGCCGGCG GAGCGAATCCAGCGCCGCGGCATGGCCGACAACTACTGGTCGATGGGCATTCCCGGACCGTG CGGGCCGTCATCGGAGATCTATTACGACCGCGGACCCGAATTCGGTCCCGCAGGCGGTCCCAT CGTCAGCGAAGACCGCTACCTCGAGGTCTGGAACCTGGTGTTCATGCAGAACGAGCGCGGAGA GGGAACCACCAAGGAGGACTACCAGATCCTCGGGCCGCTGCCCCGCAAGAACATCGACACCG

GCATGGGCGTCGAGCGGATCGCGCTGGTGCTGCAAGACGTGCACAACGTCTACGAGACCGAC CTGCTCAGGCCGGTCATCGATACCGTGGCCAGGGTCGCCGCGCGTGCCTACGACGTCGGCAA CCACGAAGACGACGTGCGGTACCGCATCATCGCAGACCACAGCCGCACCGCCGCGATCCTGAT CGGTGACGGCGTCAGCCCCGGCAACGACGGTCGCGGTTATGTGCTGCGCCGGCTGCTGCGTC GGGTGATCCGCTCCGCCAAGCTGCTGGGCATCGACGCTGCGATCGTTGGCGACCTGATGGCCA

CGGTGCGCAACGCGATGGGCCCGTCATATCCCGAACTCGTCGCCGACTTCGAGCGGATCAGCC GGATCGCGGTCGCCGAGGAGACGGCGTTCAACCGCACGCTGGCGTCGGGTTCCAGGCTGTTC GAGGAGGTGGCTAGCTCCACCAAGAAATCCGGAGCCACCGTGCTGTCCGGATCGGACGCTTTC ACGTTGCATGACACCTACGGGTTCCCGATCGAGCTCACGCTGGAGATGGCGGCCGAAACCGGT CTGCAGGTAGACGAAATCGGGTTCCGTGAGCTGATGGCCGAGCAGCGCCGCCGTGCCAAGGC

CGACGCCGCCGCGCGCAAACACGCGCATGCTGACCTGAGCGCCTACCGCGAGCTGGTTGACG CCGGCGCCACCGAGTTCACCGGATTCGACGAGTTGCGTTCCCAGGCGCGGATTCTGGGCATCT TCGTCGACGGTAAGCGGGTTCCGGTGGTGGCGCACGGTGTAGCCGGCGGAGCCGGGGAAGG GCAGCGTGTCGAACTTGTCTTAGATCGCACCCCGCTCTACGCCGAATCGGGTGGGCAGATCGC CGATGAGGGCACCATCAGCGGAACCGGTTCCAGCGAAGCTGCCCGGGCCGCGGTTACCGACG TGCAGAAGATCGCCAAAACGCTTTGGGTGCACCGAGTCAACGTGGAATCCGGGGAATTCGTCG AGGGTGACACCGTAATCGCGGCGGTGGATCCCGGGTGGCGCCGGGGTGCCACGCAGGGCCA

CTCGGGCACCCACATGGTGCATGCCGCGCTGCGACAAGTGCTGGGGCCCAACGCGGTTCAGG CGGGATCGCTGAACCGGCCGGGATATTTGCGCTTCGACTTTAACTGGCAGGGTCCGTTGACCG ACGACCAGCGCACCCAGGTCGAAGAGGTCACCAACGAGGCCGTGCAAGCGGACTTCGAGGTG CGCACGTTCACCGAACAGCTCGACAAGGCCAAGGCGATGGGTGCCATCGCGCTGTTCGGCGAG AGCTACCCCGACGAAGTGCGGGTGGTGGAGATGGGTGGACCGTTCTCGCTGGAGCTATGTGGC

GGCACCCATGTGAGCAACACGGCGCAGATCGGTCCCGTGACGATCCTGGGCGAGTCGTCGATC GGCTCCGGGGTGCGCCGGGTGGAGGCCTACGTGGGGTTGGATTCGTTTCGTCACCTGGCCAA GGAGCGTGCGTTGATGGCCGGGTTGGCCTCGTCACTGAAGGTGCCGTCCGAAGAGGTACCGG CCCGGGTGGCCAATCTAGTGGAGCGCCTGCGGGCCGCCGAGAAGGAACTCGAACGTGTCCGG ATGGCCAGCGCCCGGGCAGCCGCCACCAATGCCGCCGCCGGGGCTCAGCGGATCGGTAACGT

CCGTTTGGTGGCGCAGCGAATGTCCGGCGGGATGACCGCGGCAGACCTGCGGTCGTTGATCG GCGACATCCGCGGCAAGCTGGGTAGCGAGCCGGCGGTGGTGGCGCTGATTGCCGAGGGCGAA AGCCAAACTGTGCCGTATGCGGTCGCGGCCAATCCCGCTGCCCAGGACCTCGGAATCCGTGCC AACGACCTGGTCAAACAACTTGCGGTGGCGGTCGAAGGCCGCGGTGGCGGTAAGGCGGACCT GGCGCAGGGCTCGGGAAAGAATCCGACCGGTATCGACGCCGCGCTCGACGCGGTCCGCTCCG

AGATCGCCGTGATAGCGCGGGTCGGTTGA

>Rv2580c hisS histidyl-tRNA synthase TB.seq 2904822:2906090 MW:45118 >emb|AL123456|MTBH37RV:c2906090-2904819, hisS SEQ ID NO:102 GTGACGGAATTCTCGTCATTTTCGGCCCCCAAGGGGGTACCGGACTACGTCCCGCCCGACTCG

GCGCAGTTCGTCGCGGTGCGCGACGGGCTGCTCGCGGCGGCCCGTCAAGCCGGCTATAGCCA CATCGAGCTGCCCATCTTCGAGGACACCGCCCTGTTCGCCCGGGGCGTGGGTGAATCCACCGA CGTGGTGTCCAAGGAGATGTATACGTTCGCCGACCGTGGCGACCGCTCGGTGACGCTGCGGCC CGAGGGCACCGCCGGGGTGGTGCGTGCGGTGATCGAACACGGGCTGGATCGCGGCGCGCTG CCGGTGAAGTTGTGTTATGCGGGCCCGTTTTTCCGCTACGAGCGTCCGCAGGCCGGCCGGTAT

CGCCAGTTACAGCAAGTCGGGGTGGAGGCGATCGGCGTCGACGACCCGGCGTTGGACGCCGA GGTGATCGCCATTGCCGACGCCGGGTTCCGCTCGTTGGGTCTCGACGGGTTCCGGCTGGAAAT CACCTCCCTGGGAGACGAGAGTTGCCGTCCGCAGTACCGGGAACTGTTGCAGGAGTTCTTGTTT GGACTCGATCTCGACGAGGACACCCGCAGGCGCGCAGGGATCAATCCGCTGCGGGTGCTCGA CGACAAGCGACCCGAATTGCGTGCGATGACGGCGTCGGCGCCGGTGTTGCTGGATCATCTGTC

TGATGTCGCCAAGCAGCATTTCGACACCGTGCTCGCCCATCTGGACGCGCTTGGAGTGCCCTAT GTCATCAACCCGCGCATGGTGCGCGGCCTGGACTACTACACCAAGACCGCCTTCGAGTTCGTC CATGACGGGCTTGGTGCGCAATCGGGGATCGGCGGCGGGGGGCGCTACGACGGCCTGATGCA CCAGCTTGGCGGGCAGGACTTGTCGGGCATCGGGTTCGGGCTGGGCGTGGACCGGACCGTGC TGGCGCTGCGGGCCGAGGGCAAGACGGCGGGGGACAGCGCCCGGTGCGACGTGTTCGGCGT GCCGCTTGGCGAGGCGGCCAAGCTCAGGCTGGCGGTGCTGGCTGGACGACTGCGCGCGGCC GGGGTGCGGGTTGACCTTGCCTATGGTGATCGCGGGCTCAAAGGCGCGATGCGCGCGGCCGC

TCGTTCCGGCGCCCGTGTTGCGTTGGTAGCGGGCGACCGCGACATCGAGGCCGGGACGGTCG CAGTGAAGGACTTGACGACGGGTGAGCAAGTTTCGGTCTCGATGGATTCGGTTGTGGCCGAAG TAATTTCGCGGCTGGCTGGGTAG

>Rv2614c thrS threonyl-tRNA synthase TB.seq 2941190:2943265 MW:77123

>emb|AL123456|MTBH37RV:c2943265-2941187, thrS SEQ ID NO: 103

ATGAGCGCCCCCGCACAACCCGCCCCGGGAGTCGATGGCGGCGACCCGTCGCAAGCCCGAAT TCGGGTTCCTGCCGGGACCACCGCGGCCACCGCCGTCGGCGAAGCGGGTTTACCGCGGCGCG GTACGCCCGATGCGATCGTCGTCGTGCGCGACGCCGACGGCAACCTGCGCGACCTGAGCTGG GTGCCCGACGTCGACACCGATATCACGCCGGTGGCCGCCAACACCGACGACGGTCGCAGCGT

GATCCGCCATTCGACCGCGCACGTGTTGGCCCAAGCCGTCCAAGAGCTGTTTCCGCAGGCCAA GCTCGGCATCGGACCACCCATCACCGACGGCTTCTACTACGACTTCGACGTGCCCGAGCCGTT CACGCCCGAGGACTTGGCGGCGCTGGAAAAGCGGATGCGCCAGATCGTCAAGGAAGGCCAGC TGTTCGACCGGCGGGTCTACGAATCCACCGAACAGGCCCGCGCCGAGCTGGCCAACGAGCCC TACAAGCTGGAACTCGTCGACGACAAATCGGGTGACGCCGAGATCATGGAGGTCGGCGGTGAC

GAGCTCACCGCCTACGACAACCTCAACCCCCGCACCCGCGAGCGCGTCTGGGGCGACCTGTG CCGCGGACCGCACATCCCGACCACCAAACACATCCCGGCGTTCAAGCTCACCCGCAGCTCGGC CGCCTACTGGCGGGGCGATCAGAAAAACGCCAGCCTGCAACGGATCTACGGCACCGCGTGGG AATCCCAGGAGGCGCTCGACAGGCACCTGGAGTTCATCGAAGAGGCGCAGCGCCGCGACCAC CGCAAGCTGGGTGTCGAGCTGGACCTGTTCAGCTTCCCCGACGAAATCGGTTCCGGCCTAGCG

GTTTTCCACCCCAAGGGCGGCATCGTGCGTCGCGAACTGGAGGACTACTCGCGGCGCAAGCAC ACCGAGGCGGGCTACCAGTTCGTCAACAGCCCGCACATCACCAAGGCCCAGTTGTTCCACACC TCGGGACATCTGGACTGGTACGCCGACGGCATGTTCCCCCCGATGCACATCGACGCGGAGTAC AACGCCGACGGCTCGCTGCGCAAACCCGGCCAGGACTACTACCTCAAGCCGATGAACTGCCCG ATGCACTGCCTGATCTTCCGCGCGCGCGGGCGATCCTATCGGGAACTGCCGTTGCGGCTCTTC

GAGTTCGGCACGGTGTATCGCTACGAGAAGTCCGGTGTGGTGCACGGGTTGACCCGGGTGCGT GGGCTGACCATGGACGACGCGCACATCTTCTGCACCCGCGACCAGATGCGCGACGAGCTGCG GTCGCTGCTGCGGTTTGTGCTCGACCTGCTCGCCGACTACGGCCTCACCGACTTCTACCTCGAA CTGTCCACCAAGGACCCGGAGAAGTTCGTCGGCGCCGAGGAGGTCTGGGAGGAAGCCACCAC CGTGCTGGCCGAGGTGGGCGCCGAATCCGGGCTGGAGCTGGTGCCCGATCCAGGCGGCGCG

GCGTTCTACGGGCCCAAGATTTCAGTGCAGGTCAAAGACGCGCTGGGCCGCACCTGGCAGATG TCGACCATCCAGCTGGACTTCAACTTTCCGGAACGTTTCGGCCTGGAGTACACCGCCGCCGACG GAACCCGCCACCGCCCGGTGATGATCCACCGCGCGCTATTTGGGTCGATCGAGCGGTTCTTCG GCATTCTCACCGAGCACTACGCGGGGGCGTTCCCGGCCTGGTTGGCGCCCGTGCAGGTGGTC GGCATCCCGGTCGCCGATGAGCACGTCGCCTATCTGGAAGAGGTTGCCACGCAACTGAAGTCG CACGGGGTGCGGGCCGAGGTGGACGCCAGCGACGATCGGATGGCCAAGAAGATCGTGCACCA CACCAACCACAAGGTGCCGTTCATGGTGTTGGCGGGTGATCGTGACGTCGCCGCCGGCGCGGT

GAGTTTCCGGTTCGGTGACCGCACCCAAATCAACGGTGTGGCCCGTGACGATGCGGTGGCGGC CATTGTCGCCTGGATCGCTGACCGCGAAAATGCGGTTCCTACAGCGGAACTGGTGAAAGTGGC CGGTCGTGAGTGA

>Rv2697c dut deoxyuridine triphosphatase TB.seq 3013683:3014144 MW:15772

>emb|AL123456|MTBH37RV:c3014144-3013680, dut SEQ ID NO:104

GTGTCGACCACTCTGGCGATCGTCCGCCTCGACCCCGGGCTCCCGCTGCCCAGCCGCGCTCAC GACGGCGACGCCGGCGTTGATCTCTACAGCGCCGAAGACGTCGAGCTGGCACCTGGGCGCCG CGCCCTGGTACGGACGGGTGTTGCGGTCGCCGTCCCGTTCGGCATGGTCGGGCTGGTCCATC CGCGCTCCGGGTTGGCCACGCGGGTGGGGCTTTCGATCGTCAACAGTCCGGGCACCATCGAC

GCGGGTTATCGTGGGGAGATCAAGGTGGCCCTGATCAACTTGGACCCAGCCGCGCCCATCGTG GTACATCGCGGTGACCGAATCGCCCAGTTGCTAGTGCAACGGGTTGAGTTGGTCGAGCTGGTC GAGGTCTCGTCGTTCGACGAGGCCGGGCTGGCCTCGACATCCCGCGGCGACGGTGGCCACGG TTCCTCCGGCGGACATGCGAGTTTGTGA

>Rv2782c pepR protease/peptidase, M16 family (insulinase) TB.seq 3089045:3090358 MW:47074 >emb|AL123456|MTBH37RV:c3090358-3089042, pepR SEQ ID NO:105

ATGCCGCGACGGTCACCAGCTGACCCCGCGGCGGCGCTGGCGCCGCGGCGCACCACCCTGC CGGGCGGGCTGCGAGTGGTCACCGAATTCCTGCCCGCGGTGCACTCCGCGTCGGTCGGGGTG TGGGTCGGCGTCGGATCGCGCGACGAAGGCGCCACGGTGGCCGGGGCGGCGCACTTCCTTGA

GCATTTGCTGTTCAAGTCGACGCCCACCCGCTCTGCCGTGGACATTGCGCAGGCGATGGACGC GGTGGGCGGGGAACTGAACGCATTCACCGCCAAGGAGCACACCTGCTACTACGCCCACGTGCT CGGCAGCGACTTGCCGTTGGCCGTCGACCTGGTCGCCGATGTGGTGCTCAACGGCCGCTGTGC CGCCGACGATGTCGAGGTGGAACGTGACGTCGTCCTCGAGGAGATCGCGATGCGCGACGACG ACCCCGAGGACGCCTTGGCGGACATGTTCCTGGCGGCGTTGTTCGGCGACCACCCGGTCGGTC

GCCCGGTGATCGGCAGCGCGCAATCCGTGTCGGTGATGACGCGGGCTCAACTGCAATCGTTTC ACCTGCGGCGCTATACCCCGGAGCGGATGGTCGTCGCGGCCGCCGGCAATGTGGATCACGAC GGGCTGGTTGCGTTGGTCCGCGAGCACTTCGGGTCCCGGTTGGTCCGGGGGAGACGGCCAGT TGCGCCGCGCAAGGGTACCGGCCGGGTCAACGGCAGCCCCCGGTTGACACTGGTTAGCCGCG ACGCCGAACAGACGCATGTGTCGCTGGGCATCCGCACACCCGGGCGCGGCTGGGAGCATCGT

TGGGCACTGTCGGTGCTGCACACCGCGCTGGGCGGTGGCTTGAGTTCCCGGCTGTTCCAGGAG GTCCGCGAGACCCGCGGGCTGGCCTACTCGGTCTACTCCGCGCTGGATCTCTTCGCCGACAGC GGCGCGCTTTCGGTGTACGCGGCCTGCCTGCCCGAACGCTTCGCCGACGTGATGCGGGTGAC CGCCGATGTGCTGGAAAGCGTGGCACGCGACGGCATCACCGAGGCGGAATGCGGCATCGCCA AGGGATCGCTGCGGGGTGGGCTGGTGCTAGGGCTGGAGGATTCCAGCTCCCGGATGAGCCGG CTCGGCCGCAGCGAGTTGAACTACGGCAAGCACCGCAGCATCGAACACACCTTGCGGCAAATC GAGCAGGTCACCGTGGAGGAGGTCAACGCGGTGGCCCGCCACCTGCTGAGCAGGCGCTACGG

TGCTGCCGTTCTTGGCCCACACGGATCGAAACGATCACTGCCGCAACAACTTCGAGCGATGGTA GGGTAG

>Rv2783c gpsl pppGpp synthase and polyribonucleotide phosphorylase TB.seq 3090339:3092594 MW:79736 >emb|AL123456|MTBH37RV:c3092594-3090336, gpsl

SEQ ID NO:106

ATGTCTGCCGCTGAAATTGACGAAGGCGTGTTCGAGACGACCGCCACCATCGACAACGGGAGC TTTGGCACCCGGACCATCCGCTTCGAGACCGGCCGATTGGCCTTGCAGGCCGCCGGCGCGGT GGTCGCCTACCTCGACGACGACAACATGCTGCTGTCGGCGACCACCGCCAGCAAGAACCCCAA AGAACACTTCGACTTCTTCCCCCTCACGGTCGACGTCGAGGAGCGCATGTATGCGGCCGGCCG

CATCCCCGGTTCGTTCTTCCGTCGCGAGGGCCGACCCTCCACCGACGCGATCCTGACCTGCCG GCTCATCGACCGCCCGCTGCGCCCGTCGTTTGTCGACGGGCTGCGCAACGAGATCCAAATCGT GGTGACGATTCTCAGCCTGGATCCGGGCGATCTCTACGACGTATTGGCGATCAACGCGGCGTC GGCGTCCACCCAGCTGGGCGGTCTGCCGTTCTCCGGGCCCATCGGCGGTGTGCGGGTGGCGC TCATCGACGGCACCTGGGTCGGCTTCCCCACCGTCGACCAGATCGAGCGCGCCGTGTTCGACA

TGGTCGTGGCCGGCCGGATCGTCGAGGGTGATGTTGCCATCATGATGGTCGAAGCCGAGGCCA CCGAAAACGTCGTCGAGCTCGTCGAAGGTGGTGCCCAAGCGCCGACGGAAAGCGTGGTGGCC GCGGGCCTGGAGGCGGCCAAGCCGTTTATCGCCGCGCTGTGCACCGCGCAGCAGGAGCTTGC CGATGCCGCTGGAAAGTCGGGCAAACCGACCGTCGACTTCCCGGTGTTCCCTGACTACGGCGA AGACGTGTACTACTCGGTGTCCTCGGTGGCCACCGACGAGTTGGCCGCCGCGTTGACCATCGG

CGGTAAAGCCGAGCGCGACCAGCGCATCGACGAAATCAAGACCCAGGTTGTGCAGCGGCTCGC CGACACCTACGAGGGTCGCGAAAAGGAGGTCGGCGCCGCGTTGCGTGCCCTGACCAAAAAGCT GGTTCGGCAGCGCATCCTCACCGACCATTTCCGTATCGACGGCCGCGGCATCACCGACATTCG CGCATTGTCGGCCGAGGTGGCCGTGGTTCCGCGCGCGCACGGCAGCGCGCTGTTCGAACGCG GCGAAACCCAGATCCTGGGTGTGACCACACTCGACATGATCAAGATGGCCCAGCAGATCGACT

CGTTGGGGCCGGAGACATCGAAGCGGTACATGCACCACTACAACTTCCCGCCGTTCTCCACCG GCGAGACCGGTCGGGTCGGTTCGCCCAAGCGGCGTGAGATCGGGCACGGCGCACTGGCCGA GCGGGCCCTGGTGCCGGTGTTGCCGAGCGTCGAGGAATTCCCGTATGCCATTCGCCAGGTGTC GGAGGCTCTGGGCTCCAACGGGTCGACCTCGATGGGGTCGGTGTGCGCGTCGACGCTGGCGC TGCTCAACGCCGGGGTGCCGCTCAAGGCGCCGGTGGCCGGCATCGCGATGGGCCTGGTCTCC

GACGACATTCAAGTAGAAGGGGCGGTCGACGGCGTTGTGGAGCGTCGCTTCGTCACCCTCACC GACATCCTCGGCGCCGAAGACGCGTTCGGTGACATGGACTTCAAGGTCGCCGGGACCAAGGAC TTCGTCACCGCGCTGCAGCTGGACACCAAGCTCGACGGGATCCCTTCGCAGGTGCTTGCCGGA GCACTCGAGCAGGCCAAGGACGCCCGCCTCACGATCTTGGAGGTGATGGCTGAGGCCATCGAT AGACCCGACGAAATGAGTCCCTACGCCCCGCGGGTGACCACCATCAAGGTTCCGGTGGACAAG ATCGGGGAGGTCATCGGACCCAAGGGCAAGGTCATCAACGCCATCACCGAGGAGACCGGCGC GCAGATCTCCATCGAAGACGACGGCACCGTGTTCGTCGGCGCCACCGACGGGCCATCGGCACA

GGCCGCGATCGACAAGATCAACGCCATCGCCAACCCGCAGCTGCCGACGGTGGGCGAACGGT TCCTCGGAACCGTGGTCAAGACCACCGATTTCGGTGCCTTTGTATCGTTGCTGCCTGGCCGCGA CGGTCTGGTGCACATTTCCAAACTCGGCAAGGGCAAGCGCATCGCGAAGGTCGAGGACGTTGT CAATGTCGGTGACAAGCTGCGGGTGGAGATCGCCGACATCGACAAACGGGGCAAGATCTCCCT GATCCTGGTCGCCGACGAGGACAGCACCGCCGCCGCTACCGATGCCGCGACGGTCACCAGCT

GA

>Rv2793c truB tRNA pseudouridine 55 synthase TB.seq 3102364:3103257 MW:31821 >emb|AL123456|MTBH37RV:c3103257-3102361 , truB SEQ ID NO: 107 ATGAGCGCAACCGGCCCCGGAATCGTGGTTATCGACAAGCCCGCGGGAATGACCAGCCATGAC

GTGGTGGGGCGGTGCCGCCGCATCTTCGCCACCCGGCGGGTCGGCCACGCGGGCACCCTGG ACCCGATGGCCACCGGGGTGTTGGTGATCGGCATCGAACGCGCCACCAAGATCCTCGGTCTGC TGACGGCGGCCCCCAAGTCGTATGCCGCCACCATCCGCTTGGGTCAGACCACTTCCACCGAGG ACGCCGAAGGTCAAGTGCTGCAGTCGGTTCCGGCTAAGCACCTGACCATCGAGGCGATCGACG CCGCGATGGAGCGGCTGCGCGGTGAGATCCGGCAGGTGCCGTCGTCGGTCAGCGCGATCAAG

GTCGGTGGCCGACGCGCCTATCGGTTGGCCCGCCAGGGGCGCTCCGTGCAATTGGAAGCCCG GCCGATCCGCATCGACCGGTTCGAGCTGCTGGCCGCACGCCGGCGCGACCAGCTCATCGATAT CGATGTGGAGATCGACTGCTCCTCGGGAACCTACATCCGCGCGTTGGCACGCGACCTCGGCGA CGCGCTTGGGGTGGGAGGCCATGTGACGGCGTTGCGGCGCACCCGCGTCGGCCGCTTCGAGC TGGACCAGGCGAGATCGCTCGACGATCTCGCGGAGCGCCCCGCGCTGAGCCTGAGCCTCGAT

GAGGCCTGCCTGCTGATGTTTGCGCGCCGCGACCTGACCGCCGCGGAGGCCAGCGCGGCCGC CAACGGCCGGTCCCTGCCGGCGGTCGGTATCGACGGCGTGTACGCGGCCTGTGACGCCGACG GCCGGGTTATCGCGCTGCTGCGTGACGAGGGTTCGCGGACCAGGTCGGTGGCGGTGCTCCGC CCGGCGACGATGCACCCCGGGTAG

>Rv2797c - TB.seq 3105619:3107304 MW:58761 >emb|AL123456|MTBH37RV:c3107304-3105616, Rv2797c SEQ ID NO: 108

GTGCCACTGACCGTGGCCGATATCGATCGGTGGAACGCGCAAGCGGTCCGGGAGGTGTTTCAC GCGGCCAGTGCCCGAGCGGAGGTGACGTTCGAGGCGTCGCGTCAGTTGGCCGCGCTGTCGAT TTTTGCGAACTCGGGTGGCAAGACCGCTGAGGCGGCGGCACACCACAACGCGGGCATTCGCC

GAGACCTCGACGCCCACGGCAACGAGGCGTTGGCGGTTGCCCGGGCGGCCGACAGGGCCGC CGACGGGATTGTGAAGGTTCAGTCCGAGCTGGCCGCACTACGCCATGCCGCCGCGGCCGCCG AGCTGACGATCGATGCGCTGATCAACCGGGTGGTGCCGATCCCCGGGCTGCGATCCACCGAG GCGCAGTGGGCGCGGACGCTGGCCAAGCAAACGGAGCTGCAGGCGGAGCTGGATGCGATTAT GGCCGAGGCCAATGCCGTCGACGAGGAGCTGGCCTCAGCGGTCAATATGGCCGACGGTGACG CGCCCATCCCGGCCGATTCCGGCCCGCCGGTCGGTCCCGAGGGGCTGACCCCGACCCAGCTC GCCAGCGATGCCAACGAGGAGCGGCTGCGCGAGGAGCGCGCCCGCCTGCAGGCCCACCTCG

AGCGGTTACAGGCGGAGTATGACCAACTGAGTGTGCGGGCCGCCCGTGACTACCACAACGGCA TCCTCGACGGTGACGCGGTGGGCCGACTGGCAGCGCTTACCGACGAGCTGAGCGCCGCCAGG GGCCGGCTGGGTGAGCTCGATGCCGTCGACGAGGCGTTGAGCCGAGCACCCGAGACCTACCT GACCCAGCTGCAGATTCCCGAGGACCCAAATCAGCAGGTGCTGGCGGCCGTGGCCGTCGGTAA TCCCGACACCGCCGCCAATGTGTCGGTGACGGTTCCCGGCGTCGGGTCCACCACCCGGGGCG

CCCTGCCCGGCATGGTGACCGAAGCCCGCGACCTGCGGTCGGAGGTAATCCGGCAACTCAATG CTGCCGGCAAGCCCGCATCGGTTGCCACCATCGCCTGGATGGGCTACCACCCGCCCCCGAACC CACTCGACACCGGCAGTGCGGGCGATCTGTGGCAGACCATGACCGATGGGCAGGCACACGCG GGCGCGGCCGATCTGTCGCGGTATTTGCAGCAGGTGCGCGCCAATAACCCCAGTGGCCACCTG ACCGTGTTGGGGCACTCGTATGGGTCGCTGACGGCGTCGCTGGCGTTGCAGGACCTCGATGCC

CAGAGCGCCCATCCGGTCAACGACGTCGTGTTTTACGGCTCACCCGGCTTGGAGCTGTACAGC CCGGCGCAGCTCGGGCTCGATCACGGGCACGCTTATGTCATGCAGGCCCCCCACGACCTCATC ACCAATCTGGTGGCGCCGTTGGCGCCGCTGCACGGATGGGGCCTGGACCCCTATCTGACCCCC GGGTTCACGGAGCTGTCGTCACAGGCGGGTTTTGATCCGGGCGGGATCTGGCGTGACGGAGT GTATGCCCACGGGGACTACCCGCGGTCCTTCCTCGATGCCGCCGGCCAGCCGCAGCTGCGGA

TGTCCGGCTATAACCTGGCGGCGATCGCCGCCGGGCTGCCCGACAACACGGTGGGCCCGCCG CTGCTTCCGCCAATTCTGGGTGGCGGCATGCCGGCAGCGCCCGGCCCAGCACTGAGAGGGGG ACGTTGA

>Rv2864c ponA2 TB.seq 3175454:3177262 MW:63015 >emb|AL123456|MTBH37RV:c3177262-

3175451 , Rv2864c SEQ ID NO:109

ATGGTAACTAAAACAACATTAGCCTCAGCCACCTCAGGTTTGCTGCTGCTTGCGGTCGTCGCCAT GTCGGGCTGCACCCCGCGTCCCCAAGGGCCCGGTCCGGCGGCCGAAAAGTTCTTCGCCGCGC TGGCCATCGGTGACACCGCCTCCGCCGCCCAGCTCAGCGACAACCCCAACGAGGCGCGCGAA GCGCTGAACGCGGCCTGGGCGGGGCTGCAGGCCGCCCACCTGGATGCGCAGGTTCTCAGCGC

CAAGTACGCCGAGGACACCGGTACGGTCGCTTATCGCTTCAGCTGGCATCTGCCCAAGGACCG AATCTGGACCTATGACGGCCAGCTGAAGATGGCCCGCGACGAAGGGCGTTGGCACGTTCGCTG GACCACCAGCGGGTTGCATCCCAAGCTAGGCGAACATCAAACGTTCGCGCTACGAGCCGACCC GCCGCGGCGCGCCTCGGTGAACGAAGTCGGCGGCACCGATGTGCTGGTGCCGGGCTATCTGT ATCACTACTCGCTGGACGCCGGCCAGGCCGGCCGCGAGCTCTTCGGCACGGCACACGCGGTG

GTGGGCGCGCTGCACCCCTTCGACGACACGCTCAATGATCCGCAGCTGCTGGCCGAACAGGCC AGCTCGTCGACCCAGCCGTTGGACCTGGTCACGTTGCACGCCGACGACAGCAACCGGGTGGC CGCGGCGATCGGGCAGCTGCCTGGCGTGGTGATCACACCGCAGGCCGAGCTGCTCCCGACCG ACAAGCACTTCGCGCCGGCGGTCCTCAACGATGTCAAGAAGGCCGTCGTCGATGAACTCGACG GCAAGGCGGGTTGGCGGGTGGTGAGCGTCAACCAAAATGGCGTCGACGTCTCGGTGCTGCAC GAGGTCGCCCCATCACCTGCGTCGTCGGTTTCGATCACGTTGGATCGGGTCGTGCAAAACGCC GCGCAACACGCGGTGAACACCCGGGGCGGCAAGGCGATGATCGTCGTGATCAAGCCGTCGAC

CGGCGAGATCCTGGCGATCGCGCAGAACGCCGGGGCCGATGCGGACGGTCCGGTCGCGACCA CCGGTCTATATCCACCCGGGTCGACATTCAAGATGATCACCGCCGGTGCGGCCGTCGAGCGTG ACCTGGCTACCCCTGAGACGCTGCTGGGTTGCCCCGGGGAGATCGACATCGGGCATCGCACCA TTCCCAACTACGGTGGCTTTGATCTGGGCGTGGTGCCGATGTCACGCGCGTTTGCCAGTTCCTG CAACACCACCTTCGCCGAGCTGAGCAGCAGGCTGCCTCCCCGCGGTCTGACTCAGGCGGCCC

GGCGGTACGGGATCGGGCTTGACTACCAGGTGGACGGCATCACCACGGTGACCGGTTCGGTG CCGCCGACGGTGGACCTGGCCGAACGCACCGAGGACGGTTTCGGCCAGGGCAAGGTGCTGGC CAGCCCGTTCGGCATGGCCTTGGTGGCGGCGACGGTAGCCGCCGGGAAGACCCCGGTTCCAC AGCTGATCGCCGGCCGGCCGACGGCCGTCGAAGGCGATGCCACACCGATCAGCCAGAAGATG ATCGACGCGCTGCGGCCCATGATGCGGTTGGTGGTGACCAATGGCACCGCCAAGGAGATCGCT

GGCTGTGGCGAGGTGTTCGGTAAGACCGGCGAAGCCGAATTCCCGGGCGGATCGCATTCCTG GTTCGCCGGGTACCGTGGCGATCTGGCATTTGCGTCGCTGATCGTCGGGGGCGGTAGCTCGGA ATACGCGGTGCGGATGACCAAGGTGATGTTCGAATCGCTGCCGCCGGGGTACCTGGCGTAG

>Rv2868c gcpE TB.seq 3179368:3180528 MW:40451 >emb|AL123456|MTBH37RV:c3180528-

3179365, gcpE SEQ ID NO:110

GTGACTGTAGGCTTGGGCATGCCGCAGCCCCCGGCACCCACGCTCGCTCCCCGGCGCGCCAC CCGTCAGCTGATGGTCGGCAACGTCGGCGTGGGCAGTGACCATCCGGTCTCGGTGCAATCGAT GTGCACCACCAAAACCCACGACGTCAACTCGACATTGCAACAAATCGCCGAGCTGACCGCGGC CGGATGCGACATCGTGCGGGTGGCCTGCCCGCGCCAGGAGGACGCCGACGCGCTGGCCGAG

ATCGCCCGGCACAGCCAGATCCCGGTAGTCGCGGACATACATTTCCAGCCGCGCTACATATTCG CCGCCATCGACGCTGGATGTGCCGCGGTGCGGGTCAACCCGGGCAACATCAAGGAGTTTGACG GCCGGGTGGGTGAGGTCGCCAAGGCGGCGGGTGCGGCCGGGATCCCGATCCGAATCGGTGT CAACGCCGGTTCGCTGGACAAACGGTTCATGGAGAAGTATGGCAAAGCCACGCCCGAGGCGCT GGTTGAGTCGGCGCTGTGGGAGGCTTCGCTTTTCGAGGAGCATGGCTTCGGTGACATCAAGAT

CAGCGTCAAGCACAACGACCCGGTGGTGATGGTCGCCGCCTACGAGCTGCTTGCTGCACGGTG CGACTACCCACTGCACCTCGGTGTCACCGAGGCCGGCCCTGCTTTCCAGGGCACCATCAAGTC CGCGGTTGCCTTCGGCGCGTTGCTGTCGCGGGGCATAGGCGACACCATCCGGGTGTCGTTGTC GGCCCCGCCGGTCGAGGAAGTCAAGGTGGGCAATCAGGTTCTCGAGTCGTTGAACCTGCGGCC GCGTTCGCTCGAGATCGTGTCTTGCCCGTCGTGCGGTCGCGCGCAAGTCGACGTCTACACCCT

GGCCAACGAGGTAACCGCCGGCCTGGATGGTCTCGATGTGCCGTTGCGGGTGGCCGTGATGG GGTGTGTCGTCAATGGTCCGGGTGAAGCACGTGAGGCCGACCTGGGCGTGGCGTCCGGCAAC GGCAAAGGTCAGATCTTTGTACGGGGCGAAGTGATCAAGACCGTGCCCGAAGCACAGATCGTC

GAGACGCTGATCGAGGAGGCGATGCGGCTGGCCGCCGAAATGGGCGAGCAAGATCCGGGCGC

GACACCGAGCGGTTCGCCTATTGTGACCGTAAGCTGA

>Rv2869c - TB.seq 3180548:3181759 MW:42835 >emb|AL123456|MTBH37RV:c3181759-3180545,

Rv2869c SEQ ID NO:111

ATGATGTTTGTTACCGGCATTGTGCTGTTCGCGCTCGCGATCCTGATTTCGGTGGCCCTGCACG AATGTGGTCACATGTGGGTCGCGCGCCGCACCGGGATGAAGGTACGTCGCTATTTCGTCGGCT TTGGCCCCACGTTGTGGTCGACCCGGCGCGGCGAGACCGAATACGGTGTCAAAGCCGTTCCGC TGGGCGGCTTCTGTGACATCGCCGGCATGACCCCGGTCGAGGAACTCGACCCCGACGAACGTG

ACCGTGCGATGTACAAGCAGGCCACCTGGAAGCGGGTCGCAGTGTTATTCGCCGGGCCCGGAA TGAACCTCGCTATCTGCCTGGTGCTGATCTATGCCATCGCGCTGGTCTGGGGGCTGCCTAACCT GCATCCGCCAACCAGGGCCGTAATCGGCGAAACTGGCTGCGTTGCACAGGAAGTGAGCCAGG GCAAGCTCGAGCAGTGCACCGGGCCCGGTCCGGCGGCGCTGGCCGGAATTCGCTCCGGTGAC GTCGTGGTCAAGGTCGGTGACACCCCGGTGTCCAGTTTCGACGAGATGGCCGCCGCGGTGCG

CAAGTCACACGGCAGCGTCCCGATCGTTGTCGAGCGTGACGGCACCGCGATTGTTACCTACGT GGACATCGAATCCACCCAACGCTGGATCCCTAACGGGCAGGGCGGTGAGCTCCAGCCGGCAAC GGTCGGTGCGATTGGGGTGGGCGCCGCCCGGGTCGGGCCTGTGCGCTACGGCGTGTTCTCCG CCATGCCGGCCACATTCGCGGTCACCGGCGACCTGACCGTGGAGGTGGGCAAGGCGCTGGCC GCCCTCCCGACCAAGGTAGGTGCGCTGGTGCGGGCGATCGGCGGCGGGCAGCGTGACCCGC

AGACGCCGATAAGTGTGGTGGGCGCCAGCATCATCGGCGGCGACACCGTCGACCATGGGCTG TGGGTGGCGTTCTGGTTCTTCTTGGCCCAGCTGAACCTCATCCTGGCTGCGATCAACCTGCTGC CGTTGCTGCCGTTCGATGGCGGCCATATTGCCGTCGCGGTGTTCGAGAGGATCCGCAACATGG TCCGGTCGGCTCGTGGCAAGGTGGCGGCCGCACCGGTGAATTACCTCAAACTCTTGCCGGCGA CCTATGTGGTCTTGGTTCTTGTCGTCGGGTACATGCTCTTGACCGTCACCGCCGACCTGGTCAA

CCCGATTAGGCTTTTCCAGTAG

>Rv2870c - TB.seq 3181770:3183077 MW:45324 >emb|AL123456|MTBH37RV:c3183077-3181767, Rv2870c SEQ ID NO:112 GTGGCTACCGGTGGACGCGTCGTGATCCGGCGGCGCGGTGACAACGAGGTGGTGGCGCACAA

TGATGAGGTGACCAACTCGACCGACGGGCGCGCTGACGGCCGGTTGCGGGTGGTGGTGCTGG GCAGTACCGGCTCGATCGGCACCCAGGCGCTTCAGGTCATCGCCGACAATCCGGACCGTTTCG AGGTAGTCGGGCTGGCCGCTGGCGGCGCCCATCTGGACACGTTGCTGCGACAACGTGCGCAG ACCGGGGTGACCAATATTGCCGTCGCTGACGAGCACGCGGCGCAGCGGGTCGGCGACATCCC CTACCACGGATCCGACGCCGCCACCCGGCTGGTCGAGCAGACCGAGGCCGACGTCGTCCTCA

ATGCGCTGGTCGGCGCGTTGGGCCTGCGACCGACGTTGGCCGCGCTCAAGACGGGTGCCCGG CTGGCGCTGGCCAACAAGGAATCGCTGGTCGCCGGTGGTTCGCTGGTGCTGCGGGCGGCGCG GCCCGGTCAGATCGTGCCGGTCGACTCCGAACACTCCGCGCTGGCCCAGTGCCTGCGCGGCG GCACTCCCGACGAGGTCGCCAAGCTGGTGCTGACGGCCTCGGGAGGGCCGTTTCGGGGCTGG TCCGCGGCCGACCTCGAGCATGTCACCCCCGAGCAGGCTGGCGCGCATCCTACGTGGTCGATG GGCCCGATGAACACGCTGAATTCGGCGTCGCTGGTCAACAAGGGACTTGAGGTCATCGAAACC CACCTGCTGTTCGGCATCCCCTACGACCGCATCGATGTCGTGGTGCACCCCCAGTCGATCATCC

ATTCGATGGTCACCTTCATCGACGGTTCGACGATCGCCCAGGCCAGTCCCCCGGACATGAAGCT ACCGATTTCGTTAGCGCTGGGCTGGCCGCGTCGGGTCAGCGGCGCCGCTGCTGCCTGTGATTT CCATACCGCGTCGAGCTGGGAGTTCGAGCCGTTGGACACCGACGTCTTCCCCGCGGTCGAGTT GGCCCGGCAGGCCGGCGTAGCCGGTGGCTGCATGACCGCGGTTTACAATGCGGCGAACGAAG AAGCAGCAGCGGCGTTCCTTGCTGGCCGGATCGGCTTCCCGGCCATCGTCGGCATCATCGCCG

ACGTGTTGCACGCTGCCGACCAATGGGCCGTCGAACCCGCTACCGTGGATGACGTACTCGACG CGCAGCGCTGGGCCCGCGAGCGAGCGCAGCGCGCGGTATCTGGTATGGCTTCGGTGGCGATC GCAAGCACGGCGAAGCCGGGCGCAGCGGGTCGACACGCATCGACGTTAGAAAGGTCCTGA

>Rv2922c smc member of Smc1/Cut3/Cut14 family TB.seq 3234189:3238055 MW:139610

>emb|AL123456|MTBH37RV:c3238055-3234186, smc SEQ ID NO:113

GTGGGTGCAGGGAGTCGGTTTCCGCTGGTGGACCCGCTGCCGAGCGTTGGAGCTCGGCCTGA CCGGTTACGCGGCCAACCACGCCGACGGACGCGTGCTGGTGGTCGCCCAGGGTCCGCGCGCT GCGTGCCAGAAGCTGCTGCAGCTGCTGCAGGGCGACACGACACCGGGCCGCGTCGCCAAAGT CGTCGCCGACTGGTCGCAGTCGACGGAGCAGATCACCGGGTTCAGCGAGCGGTAATCTGGCC

CCTCGTGTACCTCAAGAGTCTGACGTTGAAGGGCTTCAAGTCCTTCGCCGCGCCGACGACTTTA CGCTTCGAGCCGGGCATTACGGCCGTCGTTGGGCCCAACGGCTCCGGCAAATCCAATGTGGTC GATGCCCTGGCGTGGGTGATGGGGGAGCAGGGGGCAAAGACGCTGCGCGGCGGCAAGATGG AAGACGTCATCTTCGCCGGCACCTCGTCGCGTGCGCCGCTGGGCCGCGCCGAAGTCACCGTTA GCATCGACAACTCCGACAACGCACTGCCTATCGAATACACCGAGGTGTCGATCACCCGAAGAAT

GTTTCGCGACGGTGCCAGCGAATACGAAATCAACGGCAGCAGTTGCCGTTTGATGGATGTGCA GGAGTTGCTGAGCGACTCCGGCATCGGCCGTGAGATGCATGTGATTGTTGGGCAAGGGAAGCT CGAGGAGATCTTGCAGTCGCGGCCTGAGGATCGGCGGGCGTTCATCGAGGAAGCCGCCGGTG TGCTCAAGCATCGCAAGCGCAAGGAAAAAGCTCTGCGCAAACTCGACACGATGGCGGCGAACC TGGCCCGGCTCACCGATCTGACCACCGAGCTCCGGCGTCAACTCAAACCGCTGGGCCGGCAG

GCCGAGGCGGCCCAGCGTGCCGCGGCCATCCAAGCCGATCTGCGCGACGCCCGGCTGCGCCT GGCGGCCGACGACTTGGTAAGCCGCAGAGCCGAACGGGAAGCGGTCTTTCAGGCCGAGGCTG CGATGCGCCGCGAGCATGACGAGGCCGCCGCCCGGCTGGCGGTGGCATCCGAGGAGCTGGC CGCGCATGAGTCCGCGGTCGCCGAACTCTCGACGCGGGCCGAGTCGATCCAGCACACTTGGTT CGGGCTGTCTGCGCTGGCCGAACGGGTGGACGCTACGGTGCGCATCGCCAGCGAACGCGCCC

ATCATCTCGATATCGAGCCGGTAGCGGTCAGCGACACCGACCCCAGAAAGCCCGAGGAGCTAG AAGCCGAGGCCCAGCAGGTGGCCGTCGCCGAGCAACAACTGTTAGCGGAGCTGGACGCGGCG CGTGCCCGACTCGATGCTGCCCGTGCAGAGCTGGCCGACCGGGAGCGCCGCGCCGCCGAGG CCGACCGGGCACACCTGGCGGCGGTCCGGGAGGAGGCGGACCGCCGTGAGGGACTGGCGCG GCTGGCTGGCCAGGTGGAGACCATGCGGGCGCGTGTCGAATCGATCGATGAGAGCGTGGCAC GGTTGTCCGAGCGGATCGAGGATGCCGCAATGCGCGCCCAGCAGACCCGAGCCGAGTTCGAA ACCGTGCAGGGCCGCATCGGTGAACTGGATCAAGGCGAGGTCGGCCTGGATGAGCACCACGA

GCGTACTGTGGCCGCGTTGCGGTTGGCCGACGAACGCGTCGCCGAGCTGCAATCCGCCGAAC GCGCCGCCGAACGCCAGGTGGCATCGCTACGGGCTCGCATCGATGCGCTCGCAGTGGGGCTA CAGCGCAAGGACGGCGCGGCGTGGCTGGCGCACAATCGCAGTGGCGCAGGGCTTTTCGGTTC GATCGCCCAATTGGTGAAGGTACGTTCCGGCTATGAAGCGGCACTGGCCGCGGCGCTCGGGC CGGCGGCCGACGCACTTGCGGTGGACGGCCTGACTGCCGCGGGTAGTGCCGTCAGCGCACTC

AAACAAGCCGACGGCGGTCGCGCGGTCCTCGTGCTGAGTGACTGGCCGGCCCCGCAAGCCCC CCAATCCGCCTCGGGGGAGATGCTGCCTAGCGGCGCCCAGTGGGCCCTAGACCTGGTCGAGT CTCCACCGCAGTTGGTTGGCGCGATGATCGCCATGCTTTCGGGTGTCGCGGTGGTCAACGACC TGACTGAGGCAATGGGCCTGGTCGAGATTCGTCCGGAGCTACGCGCGGTCACCGTTGACGGTG ATCTGGTGGGCGCCGGCTGGGTCAGCGGCGGATCGGACCGCAAGCTGTCCACCTTGGAGGTC

ACCTCCGAGATCGACAAGGCCAGGAGTGAGCTGGCCGCTGCCGAGGCGCTGGCGGCGCAATT GAATGCGGCCCTGGCCGGTGCGCTGACCGAGCAGTCCGCCCGCCAGGACGCGGCCGAGCAA GCCTTGGCCGCGCTTAACGAATCCGACACGGCCATCTCGGCGATGTACGAGCAGCTGGGCCGC CTCGGGCAGGAGGCCCGCGCGGCGGAAGAAGAGTGGAACCGGTTGCTGCAGCAGCGTACGGA ACAGGAAGCCGTGCGCACACAGACTCTCGACGACGTCATACAACTTGAGACCCAGCTGCGTAA

GGCCCAGGAGACCCAACGGGTGCAGGTGGCCCAACCGATCGACCGCCAGGCGATCAGTGCCG CTGCCGATCGCGCCCGCGGTGTCGAAGTGGAAGCCCGGCTGGCGGTGCGCACCGCCGAGGAA CGCGCCAACGCGGTTCGCGGGCGGGCCGATTCGCTGCGCCGTGCGGCTGCGGCGGAACGTG AGGCGCGGGTGCGGGCTCAGCAAGCACGCGCCGCAAGACTGCATGCGGCCGCGGTGGCCGC AGCGGTCGCCGACTGCGGACGGCTGCTGGCCGGGCGGTTGCACCGGGCGGTGGACGGGGCG

TCGCAACTGCGCGACGCGTCGGCCGCGCAACGTCAGCAGCGGTTAGCGGCGATGGCCGCGGT GCGCGACGAGGTGAACACGCTGAGCGCCCGAGTGGGGGAACTCACCGATTCGCTGCACCGCG ACGAGCTGGCTAACGCGCAGGCGGCGCTGCGTATCGAGCAGCTTGAGCAGATGGTGCTAGAG CAGTTCGGAATGGCGCCGGCCGACTTGATCACCGAATACGGTCCACATGTGGCGCTACCACCG ACCGAGCTCGAGATGGCTGAGTTCGAGCAAGCCCGCGAACGCGGCGAGCAGGTGATTGCGCC

CGCCCCCATGCCGTTCGACCGGGTTACCCAGGAGCGCCGGGCCAAACGCGCCGAGCGTGCGC TTGCCGAGTTGGGCAGGGTCAACCCGCTGGCGCTCGAAGAGTTTGCTGCCTTGGAGGAGCGCT ACAATTTCCTGTCCACCCAACTCGAGGATGTCAAGGCTGCCCGCAAGGATCTGCTGGGCGTCGT CGCCGATGTTGACGCCCGCATCCTGCAGGTGTTCAATGACGCGTTCGTAGACGTGGAACGCGA ATTTCGCGGCGTGTTCACCGCATTGTTCCCCGGTGGTGAAGGACGGCTGCGGCTGACCGAGCC

CGACGACATGCTCACCACCGGCATCGAGGTCGAAGCCCGCCCGCCGGGCAAGAAGATTACCC GACTGTCTTTGCTCTCCGGTGGCGAGAAGGCGCTGACCGCGGTGGCGATGCTGGTCGCGATCT TTCGTGCCCGTCCATCGCCGTTCTACATCATGGACGAGGTGGAGGCCGCCCTCGACGACGTGA ACCTGCGCCGACTGCTCAGCCTGTTCGAACAGCTGCGAGAGCAGTCGCAGATCATCATCATCAC CCACCAGAAGCCGACGATGGAGGTCGCGGACGCACTGTACGGCGTAACCATGCAGAACGACG GCATCACCGCGGTCATCTCGCAGCGCATGCGCGGTCAGCAGGTGGATCAGCTGGTTACCAATT CCTCGTAG

>Rv2925c rnc RNAse III TB.seq 3239829:3240548 MW:25400 >emb|AL123456|MTBH37RV:c3240548-3239826, rnc SEQ ID N0:114 ATGATCCGGTCACGACAACCCCTGCTCGACGCACTCGGTGTGGACCTCCCGGACGAGCTGCTC TCACTGGCGTTGACCCACCGCAGCTACGCCTACGAGAACGGCGGGCTGCCGACCAACGAGCGT

TTGGAGTTTCTCGGCGATGCCGTGCTAGGGCTGACCATCACCGACGCGCTGTTCCATCGTCATC CTGATCGGTCGGAGGGGGATCTGGCCAAACTGCGGGCCAGCGTAGTCAACACCCAGGCCCTG GCCGACGTCGCACGCCGCCTCTGTGCGGAAGGCCTCGGTGTTCACGTGCTATTGGGTCGCGGC GAGGCGAACACCGGCGGGGCCGACAAGTCCAGCATTCTGGCCGACGGTATGGAATCGCTGCT GGGCGCGATCTACCTGCAACACGGTATGGAGAAGGCCCGTGAGGTGATCCTGCGGCTGTTTGG

CCCGTTGCTGGACGCCGCGCCGACCCTGGGTGCGGGATTGGATTGGAAGACCAGCTTGCAGG AGCTGACTGCAGCGCGAGGGCTGGGTGCGCCGTCATACCTGGTCACCTCCACCGGCCCGGAC CACGATAAGGAATTCACCGCGGTGGTTGTCGTGATGGACAGCGAATACGGTTCAGGAGTGGGC CGGTCCAAAAAAGAAGCCGAGCAAAAAGCCGCGGCGGCCGCTTGGAAAGCCCTGGAAGTGCTC GACAACGCCATGCCGGGCAAAACCTCCGCCTAA

>Rv2934 ppsD TB.seq 3262245:3267725 MW:193317 >emb|AL123456|MTBH37RV:3262245-3267728, ppsD SEQ ID NO:115 ATGACAAGTCTGGCGGAGCGCGCGGCGCAACTGTCGCCGAACGCGCGAGCGGCCCTGGCGCG CGAGCTCGTCCGTGCGGGTACGACCTTCCCGACCGACATCTGCGAGCCGGTGGCGGTGGTGG

GCATCGGCTGTCGCTTTCCGGGGAATGTGACTGGGCCAGAGAGCTTTTGGCAGCTACTGGCCG ACGGTGTGGACACAATCGAGCAGGTGCCGCCTGATCGGTGGGATGCGGACGCGTTCTACGATC CCGATCCTTCGGCGTCGGGTCGGATGACGACGAAATGGGGTGGTTTCGTTTCCGATGTCGACG CGTTCGACGCCGACTTTTTCGGAATCACTCCTCGGGAAGCCGTGGCGATGGACCCGCAGCATC GGATGCTGCTCGAGGTTGCCTGGGAAGCGTTGGAGCACGCGGGTATTCCGCCGGATTCCTTGA

GCGGCACTCGAACCGGCGTGATGATGGGTCTGTCGTCGTGGGACTACACGATCGTCAATATCG AGCGCAGAGCCGACATCGACGCGTACCTGAGCACCGGAACCCCGCACTGTGCCGCGGTGGGG CGGATCGCGTATCTGTTGGGATTGCGTGGTCCGGCCGTCGCCGTAGATACCGCTTGTTCGTCGT CGCTGGTGGCAATTCACTTGGCGTGTCAGAGCCTTCGCCTGCGTGAAACCGACGTGGCATTGG CGGGCGGGGTGCAGCTCACCTTGTCACCGTTCACCGCCATCGCGCTGTCCAAGTGGTCGGCGC

TGTCACCGACCGGCCGATGCAACAGCTTCGACGCCAACGCGGATGGATTCGTGCGCGGCGAG GGCTGCGGCGTGGTGGTGCTCAAGCGGTTGGCCGACGCGGTGCGCGACCAGGACCGGGTGCT TGCGGTGGTCCGCGGTTCGGCAACTAACTCCGATGGTCGGTCCAACGGCATGACCGCACCGAA CGCGCTGGCGCAGCGTGACGTGATCACATCCGCCCTCAAGCTTGCGGATGTTACCCCTGACAG CGTGAACTATGTCGAAACACACGGCACCGGAACGGTGTTGGGGGACCCCATCGAGTTCGAGTC GCTGGCGGCCACTTATGGCCTGGGTAAAGGCCAGGGCGAGAGCCCGTGCGCATTGGGGTCGG TCAAGACCAACATCGGCCACCTGGAGGCGGCCGCCGGTGTGGCTGGATTCATCAAGGCGGTGC

TGGCGGTGCAACGTGGGCACATTCCCCGCAACTTGCACTTCACCCGGTGGAACCCGGCCATCG ACGCGTCGGCGACGCGGCTGTTCGTGCCGACCGAAAGCGCCCCGTGGCCGGCGGCTGCCGGT CCACGCAGGGCTGCGGTGTCATCGTTCGGCCTCAGCGGGACCAACGCGCACGTGGTGGTCGA GCAGGCACCCGACACCGCAGTAGCCGCAGCCGGCGGCATGCCGTATGTTTCGGCGCTGAACG TCTCCGGCAAGACGGCCGCGCGGGTGGCGTCGGCGGCGGCGGTGCTGGCCGACTGGATGTC

GGGGCCGGGCGCGGCGGCACCACTGGCCGACGTGGCACACACGTTGAACCGGCACCGGGCC CGGCACGCCAAGTTCGCCACCGTCATCGCGCGTGACCGCGCCGAGGCGATCGCGGGGTTGCG AGCGCTGGCGGCCGGACAACCACGCGTTGGGGTGGTGGATTGCGACCAGCATGCCGGTGGGC CTGGCCGGGTTTTTGTGTATTCGGGTCAGGGCTCGCAGTGGGCGTCGATGGGCCAGCAGTTGC TGGCCAACGAACCGGCGTTCGCCAAGGCGGTAGCCGAGCTGGATCCGATATTCGTTGACCAGG

TTGGCTTTTCGCTGCAGCAAACGCTTATCGACGGCGACGAGGTGGTGGGCATCGACCGCATCC AGCCGGTGCTGGTCGGGATGCAGTTGGCGCTGACCGAGTTATGGCGGTCCTATGGGGTGATTC CAGATGCCGTGATCGGGCACTCGATGGGTGAGGTGTCGGCGGCAGTGGTGGCCGGCGCGTTG ACGCCCGAGCAGGGCTTGCGGGTCATCACCACCCGGTCGCGGTTGATGGCGCGGCTGTCGGG GCAGGGAGCGATGGCGCTGCTCGAGCTGGATGCCGACGCCGCCGAGGCGCTGATTGCCGGCT

ATCCGCAGGTGACGCTGGCGGTGCATGCGTCACCGCGCCAGACGGTGATCGCCGGGCCGCCC GAGCAGGTGGACACGGTGATCGCGGCGGTAGCGACGCAAAACCGGTTGGCGCGCCGCGTCGA AGTCGACGTGGCCTCCCATCACCCGATCATCGATCCCATACTGCCCGAGTTGCGAAGCGCGTTA GCGGATTTGACTCCGCAGCCGCCGAGCATCCCGATCATTTCCACTACGTACGAAAGCGCGCAG CCGGTGGCGGATGCCGACTATTGGTCGGCCAACCTGCGCAACCCGGTGCGATTCCACCAGGCC

GTCACCGCCGCCGGTGTCGACCACAACACCTTCATCGAAATCAGCCCTCACCCCGTGCTCACG CACGCACTCACCGACACCCTGGATCCGGACGGCAGCCATACAGTCATGTCGACGATGAACCGC GAACTGGACCAGACGCTGTATTTCCACGCCCAACTCGCCGCGGTCGGTGTGGCTGCGTCCGAG CACACCACCGGTCGCCTTGTCGACCTGCCCCCCACACCGTGGCACCATCAGCGATTCTGGGTC ACGGATCGTTCGGCGATGTCCGAGCTGGCCGCGACCCACCCGCTCCTGGGCGCGCACATCGA

GATGCCGCGCAACGGAGACCATGTCTGGCAGACCGATGTCGGCACCGAGGTCTGTCCCTGGTT GGCAGACCACAAGGTGTTCGGTCAACCCATCATGCCGGCCGCGGGGTTCGCCGAGATCGCCTT GGCGGCGGCCAGCGAAGCCCTCGGCACAGCCGCCGACGCCGTCGCACCCAACATCGTGATCA ACCAGTTCGAGGTGGAGCAGATGCTGCCCCTCGACGGCCACACGCCGCTAACGACGCAGTTAA TTCGCGGCGGGGACAGCCAGATTCGGGTCGAGATCTATTCCCGCACGCGTGGCGGAGAGTTCT

GCCGACACGCCACGGCCAAGGTTGAACAATCGCCGCGCGAATGTGCGCACGCGCACCCGGAA GCCCAAGGTCCCGCCACCGGGACAACAGTGTCGCCGGCCGATTTTTATGCCCTGCTCCGCCAA ACCGGCCAACACCATGGTCCGGCGTTCGCGGCCTTAAGCCGGATCGTGCGCCTGGCCGATGGT TCCGCGGAAACCGAGATCAGCATTCCCGACGAGGCGCCGCGCCATCCCGGGTATCGGCTGCA CCCCGTGGTATTGGATGCGGCATTGCAAAGCGTGGGTGCCGCGATACCCGACGGCGAGATCGC GGGGTCGGCGGAAGCCAGCTATCTGCCAGTGTCGTTCGAGACCATCCGGGTGTACCGCGACAT CGGTCGGCACGTCAGGTGTCGTGCCCACCTGACAAACCTCGACGGCGGCACCGGAAAGATGG

GCAGGATCGTCCTAATCAACGACGCCGGCCACATAGCGGCCGAAGTGGACGGCATCTATCTGC GTCGTGTCGAACGCCGTGCGGTACCCCTGCCACTAGAGCAGAAGATCTTCGATGCCGAATGGA CCGAAAGCCCGATCGCAGCCGTGCCGGCTCCGGAGCCAGCTGCCGAGACGACGCGGGGAAGT TGGCTGGTACTCGCCGATGCAACGGTGGATGCGCCAGGCAAGGCCCAGGCCAAGTCGATGGC CGACGACTTCGTGCAGCAGTGGCGCTCACCGATGCGGCGGGTGCACACCGCCGATATCCACGA

CGAATCGGCGGTGCTGGCCGCATTTGCAGAAACGGCAGGCGATCCCGAGCACCCGCCGGTTG GCGTGGTGGTGTTCGTCGGCGGTGCCTCGAGTCGACTGGACGACGAGCTGGCGGCGGCGCGC GACACGGTGTGGTCGATCACCACGGTGGTTCGTGCGGTCGTCGGCACGTGGCACGGCCGATCA CCGCGGCTATGGCTGGTCACCGGGGGCGGACTTTCCGTTGCCGACGACGAGCCGGGAACACC CGCGGCGGCTTCCTTGAAAGGGCTGGTGCGGGTGCTCGCCTTCGAGCACCCGGACATGCGCA

CCACCCTGGTCGATCTGGACATCACACAAGACCCGCTGACCGCGCTGAGCGCGGAACTGCGGA ATGCCGGGAGTGGGTCGCGCCATGATGACGTGATCGCGTGGCGCGGCGAGCGCAGGTTCGTC GAACGGCTGTCGCGCGCCACGATCGATGTATCCAAAGGGCATCCGGTGGTGCGCCAGGGAGC GTCGTACGTCGTCACCGGCGGCCTCGGCGGTCTCGGCCTGGTCGTCGCTCGTTGGCTGGTGG ACCGCGGCGCCGGCCGGGTGGTGCTGGGTGGCCGCAGCGATCCCACTGACGAGCAGTGCAAC

GTCCTGGCCGAACTGCAGACCCGCGCCGAGATCGTGGTTGTCCGTGGCGACGTGGCATCGCC GGGGGTGGCAGAAAAGCTGATTGAGACGGCCCGACAGTCTGGGGGCCAATTGCGCGGCGTCG TGCACGCCGCCGCGGTCATCGAAGACAGCCTGGTGTTCTCTATGAGCAGGGACAACCTAGAAC GGGTGTGGGCACCCAAGGCCACCGGTGCGCTGCGCATGCACGAAGCCACCGCTGACTGCGAG CTCGACTGGTGGCTCGGATTCTCTTCCGCCGCTTCGCTATTGGGTTCTCCCGGGCAAGCGGCCT

ACGCGTGCGCCAGCGCGTGGCTGGACGCGCTGGTCGGATGGCGCAGGGCATCCGGCCTGCC GGCCGCGGTGATCAACTGGGGTCCGTGGTCGGAGGTAGGCGTCGCCCAGGCCTTGGTGGGCA GTGTTCTCGACACGATCAGTGTCGCAGAAGGCATCGAGGCTCTCGACTCATTGCTTGCCGCCGA CCGGATCCGCACTGGAGTGGCTCGGCTGCGTGCCGATCGGGCCCTGGTCGCATTCCCGGAGA TCCGCAGCATCAGCTACTTCACCCAGGTGGTCGAGGAGCTGGACTCGGCGGGTGACCTCGGCG

ACTGGGGCGGGCCCGACGCGCTTGCCGACCTCGACCCGGGCGAGGCGCGGCGCGCGGTGAC CGAGCGGATGTGTGCGCGCATCGCTGCGGTGATGGGCTACACTGACCAGTCGACTGTCGAACC CGCCGTGCCCTTGGACAAGCCCCTGACCGAGCTGGGGCTGGATTCTCTGATGGCGGTACGAAT ACGCAACGGCGCGCGGGCGGATTTCGGCGTGGAACCGCCGGTAGCGCTGATACTGCAAGGCG CGTCCTTGCATGACCTGACGGCGGACTTAATGCGCCAACTCGGGCTCAATGATCCCGATCCGG

CGCTCAACAACGCTGACACTATTCGCGACCGGGCGCGCCAGCGCGCGGCAGCGCGACACGGA GCCGCGATGCGGCGCCGACCTAAACCTGAAGTACAGGGAGGATAA >Rv2946c pksl TB.seq 3291503:3296350 MW:166642 >emb|AL123456|MTBH37RV:c3296350-3291500, pksl SEQ ID NO:116

GTGATTTCGGCGAGATCGGCTGAGGCGTTGACGGCGCAGGCGGGTCGACTTATGGCCCACGTG CAGGCCAACCCAGGGCTGGATCCGATCGATGTGGGGTGCTCGTTGGCCAGTCGCTCGGTGTTT

GAGCACCGAGCGGTGGTGGTCGGCGCAAGCCGTGAGCAACTGATTGCCGGGCTGGCTGGGCT CGCGGCGGGCGAGCCGGGTGCCGGCGTGGCGGTCGGTCAGCCAGGGTCGGTGGGCAAGACG GTGGTCGTGTTTCCTGGGCAGGGCGCGCAGCGCATCGGGATGGGCCGCGAGTTGTACGGCGA GTTGCCCGTGTTTGCGCAGGCATTCGATGCGGTGGCCGACGAGTTGGACCGGCATCTGCGGTT GCCGCTGCGCGACGTTATTTGGGGTGCCGATGCGGATTTGCTTGACAGCACCGAATTTGCTCAG

CCCGCGTTGTTCGCGGTGGAGGTGGCATCGTTCGCGGTGTTGCGGGATTGGGGTGTGCTTCCG GACTTCGTCATGGGTCACTCCGTTGGAGAGCTGGCGGCGGCGCACGCGGCCGGTGTGTTGAC GTTGGCGGACGCGGCGATGCTGGTGGTGGCGCGGGGCCGGTTGATGCAGGCGCTGCCGGCA GGCGGTGCGATGGTGGCGGTGGCTGCCAGTGAGGACGAGGTGGAGCCGCTGCTGGGTGAGG GTGTGGGGATCGCTGCGATCAACGCGCCCGAATCGGTGGTGATCTCCGGTGCGCAGGCCGCG

GCAAATGCGATTGCGGATCGGTTCGCCGCGCAGGGTCGGCGGGTGCACCAGTTGGCGGTCTC GCATGCGTTTCATTCGCCGTTGATGGAGCCGATGCTCGAGGAGTTCGCGCGTGTCGCGGCCCG GGTGCAGGCACGCGAGCCCCAGCTTGGGCTGGTGTCGAACGTGACGGGCGAGTTGGCCGGCC CTGATTTCGGGTCGGCGCAGTACTGGGTGGACCACGTTCGTCGGCCGGTGCGCTTCGCGGACA GTGCGCGTCATTTGCAGACCCTTGGGGCGACCCACTTCATCGAGGCCGGCCCGGGAAGTGGTT

TGACTGGCTCGATCGAGCAGTCCTTGGCCCCGGCTGAGGCGATGGTGGTGTCGATGCTGGGCA AAGACCGGCCCGAGCTGGCCTCGGCGCTCGGTGCTGCCGGTCAGGTGTTCACCACCGGTGTG CCGGTGCAGTGGTCGGCGGTGTTCGCCGGCTCGGGTGGACGGCGGGTGCAGCTGCCCACGTA TGCGTTTCAGCGACGGCGGTTTTGGGAGACGCCGGGCGCGGATGGGCCCGCCGATGCGGCCG GGTTGGGTCTGGGCGCGACCGAGCATGCCTTGTTGGGTGCGGTGGTCGAGCGGCCCGATTCT

GACGAGGTGGTGCTGACCGGCCGGTTGTCGCTTGCGGATCAGCCGTGGCTGGCCGACCACGT GGTGAACGGGGTGGTGCTGTTCCCCGGGGCGGGTTTTGTGGAGTTGGTGATCCGCGCCGGTG ATGAGGTCGGGTGCGCGCTCATCGAAGAGTTGGTGCTGGCCGCACCGTTGGTGATGCACCCGG GTGTCGGGGTTCAGGTGCAGGTGGTCGTCGGGGCTGCCGATGAATCCGGGCACCGTGCGGTG TCGGTGTATTCCCGCGGTGATCAATCCCAGGGTTGGTTGCTGAACGCCGAAGGCATGCTGGGG

GTGGCTGCCGCTGAGACGCCGATGGATTTGTCCGTGTGGCCGCCCGAGGGCGCGGAGAGTGT GGATATCTCGGACGGCTATGCGCAGTTGGCCGAGCGCGGTTATGCCTACGGCCCCGCGTTTCA GGGTCTGGTGGCGATCTGGCGGCGGGGGTCGGAGCTGTTCGCCGAAGTTGTAGCCCCCGGCG AGGCCGGCGTGGCCGTCGACCGAATGGGGATGCATCCGGCGGTGTTGGACGCGGTGCTGCAT GCCCTCGGGCTGGCCGTCGAGAAGACCCAGGCGAGCACCGAGACGAGACTGCCGTTTTGCTG

GCGTGGGGTGTCGCTGCATGCCGGCGGCGCTGGACGGGTGCGGGCCCGCTTCGCGTCCGCG GGCGCGGATGCGATTTCCGTGGACGTCTGCGACGCCACTGGGCTGCCGGTGTTGACGGTGCG CTCGCTGGTTACTCGCCCGATAACCGCAGAACAGCTGCGCGCCGCCGTGACCGCGGCCGGCG GTGCGTCCGATCAGGGGCCGCTGGAAGTGGTGTGGTCGCCGATCTCGGTGGTCAGCGGCGGC GCTAACGGGTCCGCCCCACCTGCCCCGGTGTCTTGGGCGGACTTTTGCGCCGGCAGTGATGGT GACGCCAGTGTCGTGGTGTGGGAACTCGAGTCTGCCGGTGGCCAAGCATCCTCGGTGGTGGG CTCGGTGTATGCGGCCACCCACACCGCCCTGGAGGTGTTGCAGTCCTGGCTCGGCGCGGATCG

GGCGGCCACGTTGGTGGTGTTGACCCATGGTGGCGTGGGGCTGGCTGGCGAGGACATCAGCG ACCTGGCCGCCGCCGCGGTGTGGGGCATGGCGCGTTCCGCGCAGGCCGAAAATCCCGGCCG GATCGTGTTGATCGACACCGATGCGGCGGTGGATGCCTCGGTGCTAGCCGGCGTCGGGGAAC CCCAGCTGCTGGTGCGCGGCGGCACTGTGCACGCCCCCCGGCTGTCCCCGGCCCCGGCGTTG CTAGCGTTACCGGCGGCAGAGTCGGCGTGGCGATTGGCCGCCGGTGGTGGCGGGACCCTGGA

GGATTTGGTGATCCAGCCCTGCCCGGAGGTACAGGCACCGCTACAGGCGGGGCAGGTGCGCG TGGCGGTGGCGGCCGTCGGGGTCAACTTCCGCGATGTGGTGGCCGCCCTAGGGATGTATCCC GGCCAGGCCCCACCGCTGGGTGCCGAAGGCGCCGGGGTGGTGCTTGAGACCGGTCCCGAAGT GACCGATCTTGCCGTCGGTGACGCCGTGATGGGATTCCTGGGCGGGGCCGGTCCGCTGGCGG TGGTGGATCAGCAACTGGTTACCCGGGTGCCGCAAGGCTGGTCGTTTGCTCAGGCAGCCGCTG

TGCCGGTGGTGTTCTTGACGGCCTGGTACGGGTTGGCCGATTTAGCCGAGATCAAGGCGGGCG AATCGGTGCTGATCCATGCCGGTACCGGCGGTGTGGGCATGGCGGCTGTGCAGCTGGCTCGC CAGTGGGGCGTGGAGGTTTTCGTCACCGCCAGCCGTGGCAAGTGGGACACGCTGCGCGCCAT GGGGTTTGACGACGACCATATCGGCGATTCCCGCACATGCGAGTTCGAGGAGAAGTTCCTGGC GGTCACCGAGGGCCGCGGGGTTGATGTGGTGCTCGACTCGCTGGCCGGTGAGTTCGTGGATG

CGTCGCTGCGCTTACTGGTCCGCGGTGGGCGTTTCCTCGAGATGGGCAAGACGGATATCCGCG ATGCGCAGGAGATCGCCGCTAATTATCCCGGCGTGCAGTATCGGGCGTTCGACCTGTCGGAGG CCGGCCCGGCACGCATGCAGGAGATGTTGGCCGAGGTGCGGGAGCTGTTCGACACCCGGGAG CTGCACCGGCTACCGGTCACCACGTGGGATGTGCGCTGCGCCCCGGCGGCCTTCCGGTTCATG AGCCAGGCCCGCCATATCGGCAAGGTTGTCTTAACCATGCCCTCGGCGTTGGCCGACCGGCTT

GCCGACGGCACGGTGGTGATCACCGGTGCCACCGGGGCGGTTGGTGGGGTGTTGGCCCGCCA CCTGGTTGGCGCCTATGGGGTGCGTCATCTGGTGTTGGCCAGTCGGCGGGGCGATCGCGCGG AGGGAGCGGCCGAATTGGCCGCCGACTTGACGGAGGCCGGCGCCAAGGTGCAGGTGGTGGC CTGTGACGTGGCCGATCGCGCTGCGGTAGCGGGGTTGTTTGCCCAGCTGTCGCGGGAGTACCC GCCGGTGCGCGGGGTGATTCATGCCGCCGGCGTGCTCGATGACGCAGTGATCACCTCGTTGAC

ACCGGACCGCATCGATACGGTGTTGCGGGCCAAGGTGGACGCGGCGTGGAACCTGCACCAGG CCACCAGTGACCTGGATTTGTCGATGTTTGCGCTGTGCTCATCGATCGCGGCCACGGTCGGCTC GCCGGGGCAGGGCAACTACTCGGCGGCAAACGCGTTTCTGGACGGGTTGGCCGCTCACCGGC AGGCCGCAGGGTTGGCCGGGATATCACTGGCGTGGGGTTTGTGGGAACAGCCTGGCGGCATG ACCGCGCATTTGAGCAGCCGAGATCTGGCCCGCATGAGCCGCAGCGGGCTGGCTCCGATGAG

CCCTGCCGAAGCGGTGGAATTGTTTGACGCTGCGCTGGCCATCGATCACCCTCTGGCGGTGGC CACGCTCTTGGACCGGGCTGCACTAGACGCCCGGGCCCAGGCCGGTGCGTTGCCGGCGCTGT TCAGCGGGCTCGCGCGCCGCCCACGCCGACGCCAAATCGACGACACCGGTGACGCCACCTCG TCGAAGTCGGCGCTGGCTCAACGCCTACACGGGCTGGCCGCGGACGAACAACTCGAGCTGCTA GTGGGGCTGGTGTGTCTGCAGGCAGCGGCAGTGCTGGGTAGGCCCTCCGCCGAGGACGTCGA CCCCGACACCGAATTCGGCGACCTCGGTTTCGACTCATTAACGGCTGTGGAGTTACGCAACCGC CTCAAAACCGCCACCGGACTGACGCTGCCACCTACCGTGATTTTCGATCATCCCACTCCCACTG

CGGTCGCCGAGTATGTCGCCCAGCAAATGTCTGGCAGCCGCCCAACGGAATCCGGTGATCCGA CGTCGCAGGTTGTCGAACCCGCCGCCGCGGAAGTATCGGTCCATGCCTAG

>Rv3014c ligA DNA ligase TB.seq 3372545:3374617 MW:75258 >emb|AL123456|MTBH37RV:c3374617-3372542, ligA SEQ ID N0:117

GTGAGCTCCCCAGACGCCGATCAGACCGCTCCCGAGGTGTTGCGGCAGTGGCAGGCACTGGC CGAGGAGGTGCGTGAGCACCAGTTCCGTTATTACGTGCGGGACGCGCCGATCATCAGCGACGC GGAATTCGACGAGCTGCTGCGCCGTCTGGAAGCCCTCGAGGAGCAGCATCCCGAGCTGCGCA CGCCCGATTCGCCGACCCAGCTGGTCGGCGGTGCCGGCTTCGCCACGGATTTCGAGCCCGTC GACCATCTCGAACGAATGCTCAGCCTCGACAACGCGTTCACCGCCGACGAACTCGCCGCCTGG

GCCGGCCGCATCCATGCCGAGGTCGGAGACGCCGCACATTACCTGTGTGAGCTCAAGATCGAC GGCGTCGCGCTGTCTTTGGTCTACCGCGAGGGACGGCTGACCCGGGCCTCCACCCGCGGCGA CGGGCGCACCGGCGAGGACGTCACCCTGAACGCCCGGACCATCGCCGACGTTCCCGAACGGC TCACCCCCGGCGACGACTACCCGGTGCCCGAGGTCCTCGAGGTCCGCGGCGAGGTCTTCTTCC GGCTGGACGACTTCCAGGCGCTCAACGCCAGCCTCGTCGAGGAGGGCAAGGCGCCGTTCGCC

AACCCCCGCAACAGCGCGGCGGGATCGCTGCGCCAGAAAGACCCGGCGGTCACCGCGCGCCG CCGGCTGCGGATGATCTGCCACGGGCTGGGCCACGTGGAGGGCTTTCGCCCGGCCACCCTGC ATCAGGCATACCTGGCGTTGCGGGCATGGGGACTGCCGGTTTCCGAACACACCACCCTGGCAA CCGACCTGGCCGGTGTGCGCGAGCGCATCGACTACTGGGGCGAGCACCGCCACGAGGTGGAC CACGAAATCGACGGCGTGGTGGTCAAAGTCGACGAGGTGGCGTTGCAGCGCAGGCTGGGTTC

CACGTCGCGGGCGCCGCGCTGGGCCATCGCCTACAAGTACCCGCCCGAGGAAGCGCAGACCA AGCTGCTCGACATCCGGGTGAACGTCGGCCGCACCGGGCGGATCACGCCGTTTGCGTTCATGA CGCCGGTGAAGGTGGCCGGGTCGACGGTGGGACAGGCCACCCTGCACAACGCCTCGGAGATC AAGCGCAAGGGCGTGCTGATCGGCGACACCGTGGTGATCCGCAAGGCCGGCGACGTGATCCC CGAGGTGCTGGGACCCGTCGTCGAACTGCGCGATGGCTCCGAACGCGAATTCATCATGCCCAC

CACCTGCCCGGAGTGCGGTTCGCCGTTGGCGCCGGAGAAGGAAGGCGACGCCGACATCCGTT GCCCCAACGCCCGCGGCTGCCCGGGGCAACTGCGGGAGCGGGTTTTCCACGTCGCCAGCCGC AACGGCCTAGACATCGAGGTGCTCGGTTACGAGGCGGGTGTGGCGCTCTTGCAGGCGAAGGT GATCGCCGACGAGGGCGAGCTGTTCGCGCTGACCGAGCGGGACTTGCTGCGCACCGACCTGT TCCGAACCAAGGCAGGCGAACTGTCGGCCAACGGCAAACGGCTGCTGGTCAACCTCGACAAGG

CCAAGGCGGCACCGCTGTGGCGGGTGCTGGTGGCGCTGTCCATCCGCCATGTCGGGCCGACG GCGGCCCGCGCCCTGGCCACCGAGTTCGGCAGCCTTGACGCCATCGCCGCGGCGTCCACCGA CCAGCTGGCCGCCGTCGAGGGGGTGGGGCCGACCATTGCCGCCGCGGTCACCGAGTGGTTCG CCGTCGACTGGCACCGCGAGATCGTCGACAAGTGGCGGGCCGCCGGGGTGCGAATGGTCGAC GAGCGTGACGAGAGTGTGCCACGCACGCTGGCCGGGCTGACCATCGTGGTCACCGGCTCGCT GACCGGTTTCTCCCGCGACGACGCCAAGGAGGCGATCGTGGCCCGCGGCGGCAAGGCCGCCG GCTCGGTGTCGAAGAAGACCAACTATGTCGTCGCCGGAGACTCGCCGGGATCCAAATACGACA

AGGCGGTGGAGTTGGGGGTGCCGATTCTGGACGAGGATGGGTTCCGGAGACTGCTGGCCGAC GGACCCGCGTCACGAACGTAA

>Rv3025c - NifS-like protein TB.seq 3383885:3385063 MW:40948 >emb|AL123456|MTBH37RV:c3385063-3383882, Rv3025c SEQ ID NO:118

ATGGCCTACCTGGATCACGCTGCCACCACCCCGATGCACCCCGCCGCCATCGAGGCGATGGCG GCCGTGCAGCGCACCATCGGCAATGCGTCGTCGCTGCACACCAGCGGGCGCTCGGCGCGCCG GCGGATCGAGGAGGCCCGTGAGCTGATCGCGGACAAGCTAGGCGCTCGTCCGTCCGAGGTGA TCTTCACCGCGGGCGGCACCGAAAGCGACAACCTGGCTGTCAAAGGTATCTATTGGGCACGCC GCGATGCGGAGCCGCACCGCCGTCGCATCGTCACCACCGAGGTGGAACACCACGCCGTACTG

GACTCGGTGAACTGGCTCGTGGAACACGAAGGCGCCCATGTGACCTGGCTGCCGACCGCCGC CGACGGCTCGGTGTCGGCAACTGCGCTGCGCGAGGCACTGCAGAGCCACGACGACGTCGCGC TGGTATCGGTGATGTGGGCCAACAACGAGGTCGGAACTATTCTACCGATCGCCGAAATGTCAGT TGTCGCCATGGAATTCGGCGTGCCGATGCACAGTGATGCCATTCAGGCGGTGGGACAGCTCCC GCTTGACTTCGGGGCCAGCGGGCTGTCGGCGATGAGCGTGGCCGGGCACAAATTCGGTGGCC

CGCCAGGAGTGGGTGCGTTGCTGCTGCGCCGCGACGTCACCTGCGTGCCCCTTATGCACGGC GGTGGGCAGGAGCGCGATATTCGTTCCGGCACACCCGATGTCGCCAGTGCAGTTGGAATGGCG ACGGCCGCGCAGATCGCGGTGGACGGACTCGAGGAAAACAGCGCGCGGTTACGGCTGCTGCG GGATCGTCTGGTCGAGGGTGTGCTGGCTGAGATTGACGATGTTTGCCTTAACGGCGCCGATGA CCCGATGCGGCTAGCGGGTAACGCGCACTTCACTTTCCGTGGCTGCGAAGGCGATGCGCTGTT

GATGTTGTTGGACGCTAACGGAATCGAGTGCTCAACCGGATCGGCCTGCACGGCAGGTGTAGC GCAGCCCTCGCATGTGTTGATTGCAATGGGCGTCGACGCGGCCAGCGCCCGCGGATCATTGCG TCTCTCGCTGGGGCACACCAGTGTTGAGGCTGATGTCGATGCCGCGTTGGAGGTGCTTCCCGG GGCGGTGGCACGTGCACGGCGGGCCGCCCTAGCCGCCGCGGGAGCATCCCGATGA

>Rv3080c pknK serine-threonine protein kinase TB.seq 3442656:3445985 MW: 119420 >emb|AL123456|MTBH37RV:c3445985-3442653, pknK SEQ ID NO:119

ATGACCGACGTTGATCCGCACGCGACGCGGCGGGACCTGGTCCCGAATATTCCCGCGGAACTG CTTGAGGCTGGATTCGACAATGTCGAGGAGATCGGGCGCGGCGGATTCGGCGTCGTCTACCGC TGCGTCCAGCCCTCGCTGGACCGCGCCGTCGCCGTCAAGGTATTGAGCACCGACCTGGATCGG

GACAATCTCGAGCGCTTCCTGCGCGAGCAGCGGGCCATGGGCCGCCTTTCCGGGCACCCGCA CATCGTGACCGTCTTGCAGGTGGGCGTGTTGGCGGGTGGGCGGCCCTTCATCGTGATGCCCTA CCACGCCAAGAATTCGTTGGAGACGCTGATTCGCCGGCACGGGCCGCTGGACTGGCGCGAGA CGCTGTCGATCGGCGTCAAGCTCGCGGGAGCGCTGGAAGCCGCGCATCGCGTCGGCACCCTG CACCGTGACGTGAAGCCGGGGAATATCCTGCTGACCGACTACGGGGAACCGCAGCTGACCGAT TTCGGAATCGCCAGAATCGCCGGGGGTTTCGAGACGGCGACCGGGGTGATTGCCGGTTCCCCG GCTTTCACCGCGCCGGAAGTTCTCGAAGGAGCATCGCCGACGCCCGCCTCTGACGTGTACTCC

CTGGGCGCGACGTTGTTCTGTGCGCTGACCGGCCATGCCGCCTACGAGCGCCGCAGCGGTGA GCGGGTGATCGCCCAGTTCCTGCGGATCACCTCGCAGCCGATCCCCGACCTGCGGAAGCAGG GACTGCCCGCGGACGTGGCCGCCGCCATCGAACGGGCGATGGCCCGCCATCCGGCGGATCGT CCCGCGACCGCGGCAGACGTTGGCGAGGAGCTTCGCGACGTTCAGCGCCGCAACGGCGTCAG CGTCGACGAGATGCCCCTCCCCGTCGAGCTGGGCGTGGAACGCCGACGCTCGCCCGAGGCGC

ACGCGGCGCATCGGCATACCGGCGGCGGCACCCCGACGGTCCCGACGCCTCCGACACCCGCG ACCAAGTACCGGCCGTCGGTGCCCACCGGCTCGCTGGTCACCCGCAGCCGGCTCACCGACAT CCTGCGCGCCGGCGGACGGCGCCGGCTGATCCTCATCCACGCGCCCTCGGGATTCGGCAAAA GCACCCTGGCGGCGCAATGGCGGGAAGAGCTCTCGCGCGACGGCGCCGCGGTCGCCTGGCT GACAATCGACAACGACGACAACAACGAGGTGTGGTTCTTGTCGCACCTGCTCGAGTCGATCCG

GCGGGTCCGGCCCACGCTGGCCGAGTCGTTGGGGCACGTGCTCGAAGAGCATGGGGATGACG CCGGCCGCTACGTGTTGACTTCGCTGATCGACGAAATCCACGAAAACGACGACCGGATCGCGG TGGTGATCGACGACTGGCATCGGGTGTCCGACAGCCGCACCCAAGCTGCCCTGGGTTTCCTGC TGGACAACGGATGTCACCACCTGCAGCTCATCGTGACCAGCTGGTCTCGCGCCGGTTTGCCGG TGGGCAGGTTGCGGATCGGCGACGAACTAGCCGAGATCGATTCGGCTGCTTTGCGCTTCGATA

CCGACGAGGCCGCCGCGCTGCTGAACGATGCTGGTGGTCTGCGATTGCCGCGCGCAGACGTG CAGGCGCTGACTACCTCTACCGACGGGTGGGCCGCGGCGCTGCGGCTGGCCGCGCTGTCGCT GCGCGGCGGGGGCGACGCGACCCAACTCCTGCGCGGACTTTCCGGCGCCAGTGACGTGATCC ACGAATTCCTGAGCGAAAACGTGCTGGACACCCTGGAACCCGAACTGCGCGAATTCCTACTGGT GGCATCGGTCACCGAACGCACGTGCGGCGGGCTGGCCTCGGCGCTGGCCGGGATCACCAATG

GGCGGGCGATGCTGGAAGAGGCCGAGCACCGCGGCTTGTTCCTGCAACGGACCGAAGACGAC CCGAATTGGTTTCGCTTCCACCAAATGTTCGCCGACTTTCTCCACCGTCGCCTCGAACGTGGCG GGTCGCACCGGGTGGCGGAACTGCACCGCAGGGCATCGGCCTGGTTCGCCGAGAACGGCTAC CTGCACGAAGCCGTCGACCATGCACTGGCCGCGGGCGATCCCGCGCGCGCCGTCGATCTTGT CGAGCAGGATGAAACGAACCTGCCGGAGCAGTCAAAGATGACCACACTTCTGGCAATCGTGCA

GAAACTGCCGACGTCGATGGTGGTTTCACGGGCCCGGCTCCAACTCGCCATCGCGTGGGCGAA CATTCTGCTGCAACGGCCGGCGCCGGCCACCGGTGCCCTGAATCGTTTCGAAACGGCCCTTGG CCGGGCCGAGCTTCCCGAGGCGACGCAGGCGGATCTGCGGGCCGAGGCAGACGTGTTGCGG GCGGTCGCCGAGGTGTTCGCAGACCGGGTCGAGCGCGTGGATGACCTTCTCGCCGAGGCAAT GTCGAGACCGGACACCCTGCCCCCGCGAGTCCCCGGGACCGCCGGCAACACCGCGGCGTTGG

CCGCGATCTGCCGCTTCGAGTTCGCCGAGGTATATCCACTGCTGGACTGGGCCGCGCCCTACC AGGAAATGATGGGACCGTTCGGCACCGTTTATGCGCAGTGCTTGCGCGGCATGGCGGCCAGGA ATCGGCTCGACATTGTCGCTGCGCTACAGAACTTCCGAACGGCGTTCGAGGTCGGCACGGCAG TGGGGGCCCACTCGCACGCGGCGCGGCTTGCGGGTTCGCTGCTCGCCGAATTGCTCTACGAG ACCGGCGATCTGGCCGGGGCTGGTCGTCTCATGGACGAGAGCTATCTGCTGGGTTCCGAGGG GGGTGCAGTGGACTACCTGGCCGCCAGGTACGTGATCGGCGCGCGGGTCAAGGCGGCCCAGG GGGATCATGAGGGTGCGGCTGATCGCCTGTCCACCGGAGGCGATACTGCCGTCCAGCTGGGG

CTGCCGCGCCTGGCTGCCCGAATCAACAACGAGCGGATCCGGCTGGGCATCGCGCTACCTGC GGCGGTGGCCGCCGATTTGCTGGCACCCCGCACCATCCCCCGCGACAATGGAATCGCCACCAT GACAGCCGAACTCGACGAGGACTCCGCGGTGCGCCTGTTGTCCGCCGGCGACTCCGCCGATC GTGACCAAGCCTGCCAACGGGCCGGTGCTCTCGCCGCCGCCATCGACGGTACGCGCAGACCG CTGGCGGCGCTGCAGGCGCAAATACTTCATATCGAAACGCTTGCCGCCACCGGACGGGAATCC

GATGCGCGAAACGAACTGGCGCCGGTAGCCACGAAGTGCGCCGAACTCGGGCTGTCACGTCT GCTGGTCGATGCGGGACTGGCCTAA

>Rv3106 fprA adrenodoxin and NADPH ferredoxin reductase TB.seq 3474004:3475371 MW:49342 >emb|AL123456|MTBH37RV:3474004-3475374, fprA SEQ ID NO:120

ATGCGTCCCTATTACATCGCCATCGTGGGCTCCGGGCCGTCGGCGTTCTTCGCCGCGGCATCC TTGCTGAAGGCCGCCGACACGACCGAGGACCTCGACATGGCCGTCGACATGCTGGAGATGTTG CCGACTCCCTGGGGGCTGGTGCGCTCCGGGGTCGCGCCGGATCACCCCAAGATCAAGTCGAT CAGCAAGCAATTCGAAAAGACGGCCGAGGACCCCCGCTTCCGCTTCTTCGGCAATGTGGTCGT CGGCGAACACGTCCAGCCCGGCGAGCTCTCCGAGCGCTACGACGCCGTGATCTACGCCGTCG

GCGCGCAGTCCGATCGCATGTTGAACATCCCCGGTGAGGACCTGCCGGGCAGTATCGCCGCC GTCGATTTCGTCGGCTGGTACAACGCACATCCACACTTCGAGCAGGTATCACCCGATCTGTCGG GCGCCCGGGCCGTAGTTATCGGCAATGGAAACGTCGCGCTAGACGTGGCACGGATTCTGCTCA CCGATCCCGACGTGTTGGCACGCACCGATATCGCCGATCACGCTTTGGAATCGCTACGCCCAC GCGGTATCCAGGAGGTGGTGATCGTCGGGCGCCGAGGTCCGCTGCAGGCCGCGTTCACCACG

TTGGAGTTGCGCGAGCTGGCCGACCTCGACGGGGTTGACGTGGTGATCGATCCGGCGGAGCT GGACGGCATTACCGACGAGGACGCGGCCGCGGTGGGCAAGGTCTGCAAGCAGAACATCAAGG TGCTGCGTGGCTATGCGGACCGCGAACCCCGCCCGGGACACCGCCGCATGGTGTTCCGGTTCT TGACCTCTCCGATCGAGATCAAGGGCAAGCGCAAAGTGGAGCGGATCGTGCTGGGCCGCAACG AGCTGGTCTCCGACGGCAGCGGGCGAGTGGCGGCCAAGGACACCGGCGAGCGCGAGGAGCT

GCCAGCTCAGCTGGTCGTGCGGTCGGTCGGCTACCGCGGGGTGCCCACGCCCGGGCTGCCGT TCGACGACCAGAGCGGGACCATCCCCAACGTCGGCGGCCGAATCAACGGCAGCCCCAACGAAT ACGTCGTCGGGTGGATCAAGCGCGGGCCGACCGGGGTGATCGGGACCAACAAGAAGGACGCC CAAGACACCGTCGACACCTTGATCAAGAATCTTGGCAACGCCAAGGAGGGCGCCGAGTGCAAG AGCTTTCCGGAAGATCATGCCGACCAGGTGGCCGACTGGCTAGCAGCACGCCAGCCGAAGCTG

GTCACGTCGGCCCACTGGCAGGTGATCGACGCTTTCGAGCGGGCCGCCGGCGAGCCGCACGG GCGTCCCCGGGTCAAGTTGGCCAGCCTGGCCGAGCTGTTGCGGATTGGGCTCGGCTGA >Rv3235 - TB.seq 3611296:3611934 MW:22659 >emb|AL123456|MTBH37RV:3611296-3611937, Rv3235 SEQ ID NO: 121

ATGATGGCCAGCAACCAAACCGCTGCGCAACACTCGTCTGCCACTCTCCAGCAGGCTCCTCGTT CGATCGATGATGCTGGAGGGTGCCCCTTGACCATCAGTCCTATCGCGAACTCACCGGGCGACA

CCTTCGCCGTCACACCCGTCGTCGAGTACGAGCCGCCGCCGCGAAACATCCCGCCGTGCGGG CAATCATCGCACGCAGCCCGGCGGCCGCACACCCCGCAGCTAGCTCGCCGACAACCAATCAGG CCGAGCGGCCGGGCACCGGCAGCGGTCACCTCCACGGCCAAGTCACCGCGGCTGCGTCAAGC GGGGACCTTCGCCGATGCCGCGCTACGCCGAGTGCTGGAGGTCATCGACCGCCGCCGCCCGG TGGGCCAGCTGCGCCCCCTGCTGGCACCCGGCCTCGTCGACTCCGTGCTCGCGGTGAGCCGC

ACGGCGGCCGGACACCAACAAGGCGCGGCCATGCTGCGCCGCATCCGGCTGACACCGGCCGG ACCCGACACCGCGGACACCGCCGCCGAGGTCTTCGGCACCTACAGTCGCGGGGACCGGATCC ATGCGATCGCCTGCCGGGTGGAACAACGGCCCGCCGGTAACGAAACCCGATGGCTGATGGTC GCCCTGCACATCGGGTGA

>Rv3255c manA mannose-6-phosphate isomerase TB.seq 3635040:3636263 MW:43340 >emb|AL123456|MTBH37RV:c3636263-3635037, manA SEQ ID NO: 122

GTGGAACTGCTACGTGGCGCGTTACGCACCTACGCTTGGGGATCGCGCACCGCTATCGCCGAA TTCACCGGGCGTCCGGTGCCGGCCGCTCACCCCGAGGCCGAACTATGGTTCGGTGCACACCC GGGTGATCCGGCTTGGCTGCAGACGCCGCATGGCCAAACCTCGTTGCTCGAAGCGTTGGTCGC

GGATCCGGAGGGGCAGCTCGGCTCCGCGTCGCGCGCGCGATTCGGCGATGTGTTGCCGTTCT TGGTCAAGGTGTTGGCGGCCGACGAGCCACTATCGTTGCAGGCCCATCCGAGCGCCGAGCAG GCGGTTGAGGGCTACCTGCGGGAAGAGCGAATGGGCATTCCGGTGTCCTCACCCGTCCGCAAC TACCGCGACACCAGTCACAAGCCAGAGTTATTGGTGGCGCTGCAGCCGTTCGAGGCGCTGGCC GGATTCCGGGAGGCGGCTCGCACCACCGAGCTGCTGCGGGCGCTGGCCGTATCCGACCTCGA

CCCGTTCATCGACTTGCTGAGCGAGGGGTCCGATGCCGATGGTTTGCGTGCGCTGTTCACCAC CTGGATTACCGCACCCCAGCCCGACATCGACGTGCTGGTGCCTGCCGTGCTGGACGGCGCTAT CCAGTACGTCAGCTCCGGCGCAACGGAATTTGGCGCCGAAGCCAAGACAGTGCTGGAACTCGG CGAACGTTATCCCGGCGACGCCGGTGTGCTGGCGGCGTTGTTGCTCAACCGCATCAGCTTGGC TCCTGGGGAGGCGATCTTCCTGCCGGCCGGCAACCTGCACGCCTATGTGCGTGGTTTCGGTGT

GGAAGTGATGGCCAACTCCGACAACGTGTTACGCGGTGGACTTACCCCTAAGCACGTCGATGT GCCCGAGTTGTTGCGGGTGCTGGACTTCGCCCCCACGCCGAAGGCTCGGCTGCGGCCCCCGA TCCGGCGCGAGGGGCTGGGGCTGGTCTTTGAGACGCCCACCGATGAGTTCGCGGCCACGCTA CTGGTGCTCGACGGCGATCACCTCGGCCACGAGGTCGACGCGTCGTCCGGCCATGACGGTCC ACAGATCTTGTTATGCACCGAGGGTTCGGCGACGGTGCACGGGAAGTGCGGGTCGCTCACGCT

ACAGCGCGGCACGGCCGCCTGGGTGGCGGCCGACGACGGCCCGATCCGGCTGACCGCCGGC CAACCCGCCAAGCTGTTCAGGGCGACCGTCGGGTTGTGA >Rv3264c rmlA2 glucose-1 -phosphate thymidyltransferase TB.seq 3644897:3645973 MW:37840 >emb|AL123456|MTBH37RV:c3645973-3644894, rmlA2 SEQ ID NO: 123

TTGGCAACTCACCAAGTCGATGCGGTGGTCCTGGTCGGTGGCAAGGGTACCCGACTGCGGCCG TTGACGCTGTCGGCGCCCAAGCCAATGCTGCCTACCGCCGGACTGCCGTTCCTCACCCATCTG

CTGTCGCGGATCGCCGCAGCGGGCATCGAGCACGTGATCCTGGGTACGTCCTACAAACCCGCA GTCTTCGAAGCGGAGTTCGGCGACGGGTCCGCACTGGGCCTACAGATCGAATACGTGACCGAG GAGCATCCCTTGGGGACTGGCGGCGGCATCGCCAACGTTGCCGGCAAGCTGCGCAACGACAC CGCGATGGTGTTTAACGGCGATGTGCTCTCGGGCGCGGATCTGGCCCAACTGCTGGACTTCCA CCGAAGCAATCGAGCCGATGTCACGCTGCAACTGGTGCGGGTGGGCGACCCGCGGGCATTCG

GCTGCGTACCCACCGACGAGGAGGACCGCGTAGTCGCCTTTCTGGAGAAGACGGAGGATCCG CCGACCGACCAGATCAATGCCGGCTGCTATGTCTTCGAACGCAACGTCATCGACCGGATTCCGC AGGGCCGGGAGGTTTCGGTGGAACGCGAGGTGTTCCCGGCCTTGCTCGCCGACGGCGACTGC AAGATCTACGGCTATGTCGATGCCAGCTATTGGCGGGACATGGGCACACCGGAAGACTTCGTTC GCGGATCGGCGGATCTGGTGCGCGGCATCGCCCCGTCTCCGGCCTTGCGTGGTCACCGCGGT

GAGCAGTTGGTGCACGACGGTGCGGCGGTATCTCCCGGTGCGTTGCTGATTGGCGGCACCGTC GTGGGGCGTGGTGCCGAAATCGGCCCCGGCACCAGATTGGACGGCGCGGTCATCTTCGATGG TGTCCGGGTGGAGGCCGGGTGCGTGATCGAGCGTTCGATCATCGGCTTCGGTGCTCGCATCGG ACCGCGGGCGTTGATCCGCGACGGTGTGATCGGTGACGGGGCCGACATCGGCGCGCGCTGCG AGTTGTTAAGTGGTGCCCGGGTATGGCCCGGTGTCTTTCTTCCCGACGGCGGGATCCGTTACTC

GTCCGACGTTTGA

>Rv3368c - TB.seq 3780334:3780975 MW:23734 >emb|AL123456|MTBH37RV:c3780975-3780331 , Rv3368c SEQ ID NO: 124 ATGACCCTCAACCTGTCCGTCGACGAGGTCCTGACCACTACCCGCTCGGTGCGCAAGCGTCTC

GATTTCGACAAGCCGGTGCCACGCGACGTGCTGATGGAATGCCTCGAGCTGGCGCTGCAGGCG CCCACCGGTTCCAATTCCCAAGGCTGGCAGTGGGTGTTCGTCGAGGACGCCGCCAAGAAAAAG GCGATCGCCGACGTCTACCTGGCCAACGCCCGGGGCTACCTCAGCGGGCCGGCGCCCGAGTA CCCCGACGGCGACACCCGCGGCGAGCGGATGGGGCGGGTCCGCGATTCGGCGACCTATCTCG CCGAACACATGCACCGGGCGCCGGTGCTGCTGATCCCCTGCCTGAAAGGCCGGGAAGACGAG

TCGGCGGTGGGTGGCGTGTCGTTTTGGGCCTCACTGTTCCCGGCGGTGTGGAGCTTCTGCCTG GCGCTGCGCTCCCGCGGGCTGGGTTCGTGCTGGACGACGCTGCACCTGCTCGACAACGGCGA GCACAAGGTGGCCGACGTGCTCGGCATTCCCTACGACGAATACAGCCAAGGCGGGCTGCTTCC GATCGCCTACACACAAGGCATCGACTTCCGGCCGGCCAAGCGGCTGCCGGCCGAGAGCGTGA CGCACTGGAACGGCTGGTAA >Rv3382c lytB1 TB.seq 3796447:3797433 MW:34667 >emb|AL123456|MTBH37RV:c3797433- 3796444, lytB SEQ ID NO:125

ATGGCTGAGGTGTTCGTGGGACCGGTCGCACAGGGATACGCTTCGGGTGAAGTCACGGTGCTG TTGGCGTCGCCGCGGTCGTTTTGCGCCGGTGTAGAGCGTGCTATCGAGACGGTCAAGCGAGTG CTTGACGTGGCCGAAGGCCCGGTGTATGTGCGCAAGCAAATCGTGCACAACACTGTTGTGGTT

GCCGAGTTGCGGGACCGGGGAGCAGTGTTCGTCGAGGATCTCGACGAGATTCCCGATCCGCC GCCGCCGGGGGCGGTCGTGGTGTTCTCCGCGCATGGGGTTTCCCCGGCGGTGCGCGCGGGC GCTGATGAGCGGGGACTGCAGGTCGTCGACGCGACCTGCCCACTGGTGGCGAAAGTCCACGC TGAAGCCGCACGGTTTGCCGCGCGCGGTGACACGGTGGTCTTCATCGGGCACGCCGGACATG AGGAGACCGAAGGCACGCTTGGCGTCGCTCCGCGGTCAACATTATTGGTGCAGACACCCGCTG

ATGTGGCAGCGTTGAACCTGCCCGAGGGTACCCAGCTATCGTATCTGACCCAGACAACCCTGG CACTTGATGAAACTGCCGATGTCATTGATGCGCTGCGCGCGAGGTTTCCGACGTTGGGCCAACC CCCCTCTGAAGACATCTGCTATGCCACCACGAACAGACAGCGTGCGCTGCAATCGATGGTCGGT GAATGTGACGTTGTGTTGGTGATTGGCTCGTGCAATTCGTCGAATTCGCGGCGTCTGGTCGAGT TGGCGCAGCGAAGTGGGACGCCGGCCTACTTGATTGACGGGCCTGATGACATTGAGCCCGAAT

GGCTGTCGTCGGTCTCGACGATCGGTGTCACCGCGGGAGCCTCCGCGCCGCCACGACTGGTG GGGCAGGTGATTGATGCACTTCGCGGATACGCCTCGATCACCGTGGTGGAACGCTCGATAGCG ACCGAGACGGTGCGATTCGGCCTTCCCAAACAGGTTCGCGCGCAATGA

>Rv3418c groES 10 kD chaperone TB.seq 3836985:3837284 MW: 10773

>emb|AL123456|MTBH37RV:c3837284-3836982, groES SEQ ID NO:126

GTGGCGAAGGTGAACATCAAGCCACTCGAGGACAAGATTCTCGTGCAGGCCAACGAGGCCGAG ACCACGACCGCGTCCGGTCTGGTCATTCCTGACACCGCCAAGGAGAAGCCGCAGGAGGGCAC CGTCGTTGCCGTCGGCCCTGGCCGGTGGGACGAGGACGGCGAGAAGCGGATCCCGCTGGACG TTGCGGAGGGTGACACCGTCATCTACAGCAAGTACGGCGGCACCGAGATCAAGTACAACGGCG

AGGAATACCTGATCCTGTCGGCACGCGACGTGCTGGCCGTCGTTTCCAAGTAG

>Rv3423c air TB.seq 3840193:3841416 MW:43357 >emb|AL123456|MTBH37RV:c3841416-3840190, air SEQ ID NO:127 GTGAAACGGTTCTGGGAGAATGTCGGAAAGCCAAACGACACGACAGATGGGCGGGGCACGACT

TCGTTGGCCATGACACCGATATCCCAGACACCTGGCCTCCTCGCCGAGGCCATGGTGGATCTG GGCGCTATTGAACACAACGTGCGGGTGCTGCGTGAGCACGCCGGCCACGCGCAGCTGATGGC GGTGGTCAAGGCCGACGGCTACGGTCACGGTGCTACGCGCGTCGCCCAAACCGCCCTGGGAG CCGGTGCGGCCGAACTCGGCGTCGCCACCGTCGACGAGGCGCTAGCGCTGCGCGCTGATGGC ATTACCGCACCGGTGCTGGCCTGGCTGCATCCGCCCGGCATCGACTTCGGGCCCGCGCTGCTG

GCCGACGTGCAGGTCGCGGTGTCCTCGCTGCGCCAACTCGACGAACTGTTGCACGCGGTGCG CCGGACCGGCCGGACGGCGACGGTGACCGTCAAGGTGGATACCGGGCTGAACCGCAATGGCG TGGGACCGGCACAATTCCCGGCCATGCTGACCGCGTTACGCCAAGCCATGGCCGAGGACGCC GTCCGGCTGCGGGGGCTGATGTCGCATATGGTTTACGCCGACAAGCCTGACGATTCCATCAAC GATGTTCAGGCCCAACGGTTTACCGCCTTTCTGGCGCAGGCCCGCGAACAAGGGGTGCGGTTC GAGGTGGCGCATCTATCGAACTCATCAGCAACTATGGCGCGCCCCGACCTGACGTTCGACCTG GTGCGGCCGGGCATCGCGGTGTATGGGCTAAGCCCGGTACCCGCCCTCGGTGACATGGGGCT

GGTGCCGGCGATGACCGTGAAATGTGCTGTTGCGCTGGTGAAATCGATTCGTGCGGGGGAGGG CGTGTCGTATGGGCACACATGGATCGCGCCACGCGACACCAATCTGGCGCTGCTGCCGATCGG TTACGCAGACGGCGTGTTCCGGTCGCTGGGCGGGCGGCTGGAGGTGCTGATCAACGGCAGAC GATGCCCCGGTGTGGGGCGGATCTGCATGGACCAGTTCATGGTCGACCTGGGCCCCGGGCCG CTTGATGTGGCCGAAGGCGACGAGGCGATTTTGTTCGGGCCGGGCATCCGGGGTGAGCCCAC

GGCTCAGGACTGGGCCGATCTTGTCGGCACCATCCACTACGAAGTGGTCACCAGCCCGCGAGG ACGTATCACCAGGACCTATCGCGAGGCTGAAAACCGTTGA

>Rv3490 otsA [alpha],-trehalose-phosphate synthase TB.seq 3908232:3909731 MW:55864 >emb|AL123456|MTBH37RV:3908232-3909734, otsA SEQ ID NO:128

ATGGCTCCCTCGGGAGGCCAGGAGGCGCAGATTTGCGATTCGGAGACCTTCGGGGACTCTGAC TTCGTGGTGGTAGCCAATCGACTGCCCGTCGATCTGGAGCGTCTTCCCGACGGCAGCACAACC TGGAAACGCAGCCCCGGAGGCTTGGTCACCGCCTTGGAGCCGGTGCTGCGGCGTCGGCGCGG GGCCTGGGTCGGCTGGCCCGGCGTTAACGACGACGGGGCCGAACCCGACCTCCACGTGCTGG ACGGCCCCATCATCCAAGACGAGCTGGAACTTCATCCGGTACGGCTGAGCACCACGGACATAG

CTCAGTACTACGAGGGATTCTCCAACGCCACACTGTGGCCGCTGTACCACGACGTCATCGTCAA GCCGCTCTACCACCGCGAATGGTGGGATCGCTACGTCGACGTCAACCAGCGCTTTGCCGAGGC CGCGTCGCGCGCCGCCGCCCACGGCGCAACCGTGTGGGTACAGGACTACCAGCTGCAGCTGG TACCGAAGATGCTGCGCATGCTGCGGCCCGATCTGACCATCGGTTTC I I I I I GCACATCCCGTT CCCGCCGGTAGAGCTGTTTATGCAGATGCCGTGGCGCACCGAGATCATCCAGGGCCTACTGGG

CGCCGACCTGGTGGGCTTCCATCTTCCGGGCGGTGCCCAGAATTTCCTGATCCTGTCCCGGCG TCTGGTCGGCACCGACACTTCCCGCGGAACCGTCGGTGTGCGGTCGCGGTTCGGTGCGGCGG TGCTCGGGTCCCGCACCATACGAGTTGGCGCCTTTCCTATCTCGGTTGACTCCGGCGCGCTCG ACCACGCTGCCCGCGACCGCAACATCAGGCGCCGGGCCCGCGAGATTCGCACCGAACTGGGA AATCCGCGCAAGATCCTGCTCGGTGTTGACCGGCTCGACTACACCAAGGGCATCGACGTACGG

CTGAAGGCCTTTTCCGAGCTGCTGGCCGAGGGCCGCGTCAAACGCGACGACACCGTCGTGGTC CAGCTGGCTACCCCGAGCCGCGAGCGGGTGGAGAGCTACCAGACGCTGCGCAACGACATCGA ACGCCAGGTCGGCCACATTAACGGCGAGTACGGTGAGGTTGGCCATCCGGTAGTGCATTACCT GCATCGACCGGCTCCGCGCGACGAGCTTATCGCTTTCTTCGTGGCCAGCGACGTCATGCTGGT CACCCCACTACGCGACGGGATGAACCTGGTGGCCAAGGAGTACGTCGCTTGCCGCAGCGATCT

TGGCGGTGCCCTGGTGCTCAGCGAATTCACCGGGGCCGCAGCCGAACTCCGGCACGCATACCT GGTCAACCCGCACGACCTGGAAGGCGTCAAGGACGGGATAGAGGAAGCGCTCAACCAGACGG AGGAGGCGGGCCGGCGGCGAATGCGGTCGCTGCGACGCCAAGTGCTCGCCCACGACGTGGA CCGCTGGGCACAGTCGTTTCTCGACGCTCTCGCCGGGGCACACCCGAGGGGCCAAGGCTAA

>Rv3598c lysS lysyl-tRNA synthase TB.seq 4041423:4042937 MW:55678 >emb|AL123456|MTBH37RV:c4042937-4041420, lysS SEQ ID NO:129

GTGAGTGCCGCTGACACAGCAGAAGACCTTCCTGAGCAGTTCCGGATTCGCCGGGACAAGCGC GCTCGCTTGCTGGCCCAGGGGCGCGATCCCTATCCCGTCGCGGTGCCGCGCACTCACACGTTG GCCGAGGTTCGCGCCGCCCACCCTGACTTGCCGATCGATACCGCGACCGAAGACATCGTCGGC GTCGCGGGCCGAGTGATCTTTGCGCGCAACTCGGGAAAGCTATGCTTTGCGACACTTCAGGAC GGCGATGGTACCCAGCTGCAAGTGATGATCAGCCTCGACAAGGTCGGCCAGGCTGCTCTCGAC

GCATGGAAAGCCGATGTCGACCTGGGCGACATCGTCTACGTGCATGGCGCGGTGATCAGTTCG CGCCGCGGCGAGCTGTCCGTCCTGGCGGATTGCTGGCGGATCGCCGCCAAGTCGCTGCGGCC GCTTCCCGTCGCGCACAAAGAGATGAGTGAAGAGTCGCGGGTTCGTCAGCGCTATGTTGACCT CATAGTTCGACCGGAAGCGCGCGCGGTGGCTCGACTACGGATCGCCGTCGTCCGCGCGATCC GGACGGCGCTTCAACGTCGTGGGTTCCTGG AAGTCGAG ACGCCCGTCTTGCAG ACGTTAGCCG

GTGGTGCGGCGGCCCGTCCGTTCGCCACTCATTCCAATGCCCTAGACATCGATCTGTACCTGCG GATCGCGCCGGAACTGTTCCTCAAGCGCTGCATCGTGGGTGGTTTCGACAAGGTCTTCGAACTT AATCGAGTGTTCCGAAACGAAGGAGCCGATTCCACGCATTCTCCGGAATTCTCCATGCTGGAGA CCTACCAGACCTACGGAACCTATGACGATTCGGCAGTCGTCACCCGGGAGCTTATTCAAGAGGT GGCCGATGAGGCGATCGGAACCAGACAACTGCCGTTGCCCGACGGCAGTGTCTATGACATCGA

CGGAGAATGGGCGACTATACAAATGTACCCGTCGCTGTCTGTGGCGCTCGGTGAAGAGATCAC ACCGCAGACGACGGTCGATCGCTTACGTGGGATCGCCGATAGCCTTGGCCTGGAGAAAGACCC AGCGATTCATGACAACCGTGGCTTCGGCCACGGCAAACTCATCGAGGAACTCTGGGAGCGCAC AGTGGGCAAGAGCTTGAGCGCACCCACATTTGTCAAGGATTTTCCGGTTCAGACAACGCCTTTG ACCCGTCAGCACCGCAGTATCCCCGGCGTAACCGAGAAGTGGGACCTCTATCTGCGCGGAATC

GAACTTGCCACCGGCTACTCGGAATTAAGCGACCCGGTAGTCCAGCGGGAGAGATTCGCCGAC CAGGCCCGTGCCGCGGCCGCTGGCGATGACGAAGCGATGGTGCTTGACGAGGATTTTCTGGCC GCTCTGGAGTACGGCATGCCACCGTGCACCGGAACCGGAATGGGTATCGATCGGTTGTTGATG TCTTTGACTGGGTTGTCAATTAGGGAGACAGTTTTGTTCCCGATTGTTCGACCACACTCCAACTG A

>Rv3600c - similar to Bacillus subtilis protein YacB TB.seq 4043041 :4043856 MW:29274

>emb|AL123456|MTBH37RV:c4043856-4043038, Rv3600c SEQ ID NO:130

GTGCTGCTGGCGATTGACGTCCGCAACACCCACACCGTTGTGGGCCTGCTGTCCGGAATGAAA GAGCACGCAAAGGTCGTGCAGCAGTGGCGGATACGCACCGAATCCGAAGTCACCGCCGACGAA

CTGGCACTGACGATCGACGGGCTGATCGGCGAGGATTCCGAGCGGCTCACCGGTACCGCCGC CTTGTCCACGGTCCCGTCCGTGCTGCACGAGGTGCGGATAATGCTCGACCAGTACTGGCCGTC GGTGCCGCACGTGCTGATCGAGCCCGGAGTACGCACCGGGATCCCTTTGCTCGTCGACAACCC GAAGGAAGTGGGCGCAGACCGCATCGTGAACTGTTTGGCCGCCTATGACCGGTTCCGGAAGGC CGCCATCGTCGTTGACTTTGGATCCTCGATCTGTGTTGATGTTGTATCGGCCAAGGGTGAATTTC TTGGCGGCGCCATCGCGCCCGGGGTGCAGGTGTCTTCCGATGCCGCGGCGGCCCGCTCGGCG GCATTGCGCCGCGTTGAACTTGCCCGCCCACGTTCGGTGGTTGGCAAGAACACCGTCGAATGC

ATGCAAGCCGGTGCGGTGTTCGGCTTCGCCGGGCTGGTAGACGGGTTGGTAGGCCGCATCCG CGAGGACGTGTCCGGTTTCTCCGTCGACCACGATGTCGCGATCGTGGCTACCGGGCATACCGC GCCCCTGCTGCTGCCGGAATTGCACACCGTCGACCATTACGACCAGCACCTGACCTTGCAGGG TCTGCGGCTGGTGTTCGAGCGTAACCTCGAAGTCCAGCGCGGCCGGCTCAAGACGGCGCGCT GA

>Rv3606c folK 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase TB.seq

4048181 :4048744 MW:20732 >emb|AL123456|MTBH37RV:c4048744-4048178, folK SEQ ID NO:131 ATGACGCGGGTAGTGCTCTCGGTTGGCTCCAACCTGGGTGACCGCCTGGCACGATTGCGGTCG

GTCGCCGACGGTCTCGGCGATGCGTTGATTGCGGCTTCCCCGATATATGAGGCCGACCCCTGG GGTGGGGTGGAGCAGGGGCAGTTCCTCAATGCGGTGCTGATCGCCGACGATCCTACCTGCGAA CCGCGGGAGTGGCTGCGGCGGGCGCAGGAGTTCGAGCGCGCTGCGGGCAGGGTGCGTGGCC AGCGCTGGGGTCCACGAAATCTCGACGTCGACCTGATCGCCTGCTACCAGACCTCGGCCACCG AGGCTCTGGTCGAAGTGACCGCGCGGGAGAACCACCTCACGCTGCCGCACCCACTGGCGCAT

CTGCGGGCCTTTGTGTTGATCCCGTGGATTGCCGTCGACCCAACGGCGCAGCTGACGGTTGCC GGGTGCCCGCGGCCCGTCACGCGACTGCTGGCCGAGCTGGAGCCCGCCGACCGCGACAGTGT GCGGTTGTTTAGGCCGTCGTTCGATCTGAATAGCAGACACCCCGTCAGTCGGGCACCGGAAAG CTGA

>Rv3607c folX may be involved in folate biosynthesis TB.seq 4048744:4049142 MW:14553

>emb|AL123456|MTBH37RV:c4049142-4048741 , folX SEQ ID NO:132

ATGGCTGACCGAATCGAACTGCGCGGCCTGACCGTGCATGGTCGGCACGGGGTCTACGACCAC GAGCGAGTGGCCGGGCAGCGGTTTGTCATCGATGTCACCGTGTGGATAGACCTGGCCGAGGC CGCCAACAGCGACGACTTGGCCGACACCTATGACTACGTGCGGCTGGCTTCGCGGGCGGCCG

AGATCGTCGCCGGACCCCCGCGGAAGCTGATCGAAACGGTCGGGGCCGAGATCGCTGATCAC GTGATGGACGACCAGCGAGTGCATGCCGTTGAGGTGGCGGTACACAAGCCGCAGGCGCCCATT CCGCAGACGTTCGACGATGTGGCGGTGGTGATCCGACGCTCACGGCGCGGCGGCCGCGGTTG GGTAGTCCCGGCGGGCGGCGCGGTATGA

>Rv3608c folP dihydropteroate synthase TB.seq 4049138:4049977 MW:28812 >emb|AL123456|MTBH37RV:c4049977-4049135, folP SEQ ID NO: 133 GTGAGTCCGGCGCCCGTGCAGGTGATGGGGGTTCTAAACGTCACGGACGACTCTTTCTCGGAC GGCGGGTGTTATCTCGATCTCGACGATGCGGTGAAGCACGGTCTGGCGATGGCAGCCGCAGGT GCGGGCATCGTCGACGTCGGTGGTGAGTCGAGCCGGCCCGGTGCCACTCGGGTTGACCCGGC GGTGGAGACGTCTCGTGTCATACCCGTCGTCAAAGAGCTTGCAGCACAAGGCATCACCGTCAG CATCGATACCATGCGCGCGGATGTCGCTCGGGCGGCGTTGCAGAACGGTGCCCAGATGGTCAA

CGACGTGTCGGGTGGGCGGGCCGATCCGGCGATGGGGCCGCTGTTGGCCGAGGCCGATGTG CCGTGGGTGTTGATGCACTGGCGGGCGGTATCGGCCGATACCCCGCATGTGCCTGTGCGCTAC GGCAACGTGGTGGCCGAGGTCCGTGCCGACCTGCTGGCCAGCGTCGCCGACGCGGTGGCCGC AGGCGTCGACCCGGCAAGGCTGGTGCTCGATCCCGGGCTTGGATTCGCCAAGACGGCGCAAC ATAATTGGGCGATCTTGCATGCCCTTCCGGAACTGGTCGCGACCGGAATCCCAGTGCTGGTGG

GTGCTTCGCGCAAGCGCTTCCTCGGTGCGTTGTTGGCCGGGCCCGACGGCGTGATGCGGCCA ACCGATGGGCGTGACACCGCGACGGCGGTGATTTCCGCGCTGGCCGCACTGCACGGGGCCTG GGGTGTGCGGGTGCATGATGTGCGGGCCTCGGTCGATGCCATCAAGGTGGTCGAAGCGTGGAT GGGAGCGGAAAGGATAGAACGCGATGGCTGA

>Rv3609c folE GTP cyclohydrolase I TB.seq 4049977:4050582 MW:22395 >emb|AL123456|MTBH37RV:c4050582-4049974, folE SEQ ID NO: 134

ATGTCGCAGCTGGATTCGCGCAGCGCATCTGCTCGTATCCGTGTGTTCGACCAGCAACGTGCC GAGGCCGCGGTGCGCGAATTGCTGTACGCGATCGGCGAGGATCCGGATAGGGACGGCTTGGT AGCCACCCCGTCCCGGGTTGCCCGGTCATACCGCGAAATGTTCGCCGGGCTCTACACCGACCC

CGACTCGGTGTTGAACACCATGTTCGACGAAGACCACGACGAGCTGGTGTTGGTCAAGGAAATC CCTATGTACTCCACCTGCGAACACCACCTGGTGGCGTTCCACGGTGTGGCCCACGTCGGCTAC ATCCCGGGCGACGACGGCAGGGTGACCGGCTTGTCAAAGATCGCGCGACTGGTCGATCTGTAC GCCAAGCGACCTCAGGTCCAGGAGCGGCTCACCAGTCAGATCGCCGATGCCCTGATGAAAAAA CTCGATCCACGCGGGGTAATCGTGGTGATCGAGGCTGAGCATCTGTGCATGGCGATGCGCGGG

GTTCGCAAGCCCGGCTCGGTCACCACTACGTCGGCGGTGCGCGGACTGTTCAAAACCAATGCC GCTTCTCGAGCCGAAGCGCTCGACCTCATTTTGCGGAAGTGA

>Rv3610c ftsH inner membrane protein, chaperone TB.seq 4050601 :4052880 MW:81987 >emb|AL123456|MTBH37RV:c4052880-4050598, ftsH SEQ ID NO:135

ATGAACCGGAAAAACGTGACTCGCACCATAACAGCGATCGCCGTCGTGGTGCTGCTCGGCTGG TCGTTCTTTTACTTCAGCGACGACACCCGCGGCTACAAGCCCGTTGATACCTCGGTGGCGATAA CACAGATCAACGGCGACAACGTCAAGAGCGCACAGATCGACGATCGCGAGCAACAGCTGCGGC TGATCCTGAAGAAGGGTAACAACGAGACCGACGGGTCCGAGAAGGTCATCACCAAGTACCCCA CCGGGTACGCCGTCGACCTGTTCAACGCGCTCAGCGCCAAAAACGCGAAGGTCAGCACGGTCG

TCAACCAGGGCAGCATCCTGGGCGAGCTGCTGGTCTACGTGCTGCCGCTGCTGTTGCTGGTGG GGCTGTTCGTGATGTTCTCCCGCATGCAAGGCGGCGCCCGGATGGGCTTCGGGTTCGGCAAGT CACGCGCCAAGCAACTGAGCAAGGACATGCCCAAGACCACCTTCGCCGACGTCGCAGGTGTCG ACGAGGCGGTCGAGGAGCTCTACGAGATCAAGGACTTCCTGCAGAACCCCAGCAGGTACCAAG CGCTGGGCGCCAAGATCCCCAAAGGCGTGCTGCTCTACGGGCCGCCGGGAACCGGTAAGACG TTGCTGGCTCGTGCGGTGGCCGGCGAAGCCGGAGTGCCGTTCTTCACCATCTCCGGCTCCGAC TTCGTCGAAATGTTCGTCGGCGTCGGCGCATCCCGTGTCAGAGACCTGTTCGAGCAGGCCAAG

CAGAACAGCCCGTGCATCATCTTCGTCGACGAGATCGACGCCGTCGGCCGACAAAGAGGCGCC GGGCTGGGCGGCGGTCACGACGAGCGTGAGCAGACCCTCAACCAGTTGCTAGTCGAAATGGA CGGTTTTGGCGATCGCGCCGGCGTCATCCTGATCGCGGCCACCAACCGGCCCGACATCCTGGA CCCGGCGCTGTTGCGGCCGGGCCGCTTCGACCGCCAGATCCCGGTATCCAACCCCGATCTGG CGGGTCGGCGGGCGGTGCTGCGCGTGCACTCCAAGGGCAAGCCGATGGCCGCGGACGCCGA

CCTCGACGGACTGGCCAAGCGGACCGTCGGCATGACCGGAGCCGACCTGGCCAACGTCATCA ACGAGGCGGCGCTGCTGACCGCCCGGGAGAACGGCACCGTCATCACCGGTCCCGCCCTCGAG GAAGCGGTGGACCGGGTGATCGGCGGCCCGCGCCGCAAAGGCCGGATCATCAGCGAGCAGGA GAAGAAGATCACCGCCTATCACGAGGGCGGGCACACCCTGGCCGCTTGGGCGATGCCCGATAT CGAGCCGATTTATAAGGTGACGATCCTGGCGCGCGGGCGTACCGGCGGGCACGCGGTGGCGG

TGCCGGAAGAAGACAAGGGCCTGCGGACCCGCTCGGAAATGATCGCGCAACTGGTGTTCGCGA TGGGTGGGCGCGCCGCCGAAGAACTGGTGTTTCGTGAGCCGACCACCGGCGCGGTGTCCGAC ATCGAGCAGGCCACCAAGATAGCGCGCTCAATGGTCACCGAATTTGGAATGAGCTCCAAGCTG GGCGCGGTCAAATACGGCTCCGAACACGGCGACCCGTTCCTCGGACGTACCATGGGCACCCAG CCGGACTACTCCCACGAGGTCGCCCGCGAGATCGACGAAGAGGTCCGCAAGCTTATCGAGGCG

GCGCATACCGAAGCGTGGGAAATCCTGACCGAATACCGCGACGTGCTGGACACTTTGGCCGGC GAGCTGCTGGAAAAGGAGACCCTGCACCGACCCGAGCTGGAAAGCATCTTCGCTGACGTCGAA AAGCGGCCGCGGCTCACCATGTTCGACGACTTCGGTGGCCGGATCCCGTCGGACAAACCGCCC ATCAAGACACCCGGCGAGCTCGCGATCGAACGCGGCGAACCTTGGCCCCAGCCGGTCCCCGA GCCGGCGTTCAAGGCGGCGATTGCGCAGGCTACCCAAGCCGCTGAGGCCGCCCGGTCCGACG

CCGGCCAAACCGGGCACGGCGCCAACGGTTCGCCCGCCGGCACCCACCGGTCCGGTGACCGC CAGTACGGCTCCACCCAGCCTGACTACGGTGCCCCGGCGGGCTGGCATGCGCCGGGATGGCC CCCAAGGTCATCTCATCGGCCCAGCTATAGCGGTGAACCGGCACCGACGTATCCGGGTCAGCC CTACCCGACCGGTCAAGCCGATCCGGGTTCCGATGAGTCCTCGGCGGAGCAGGATGACGAGGT CAGTCGGACCAAGCCGGCCCACGGCTGA

>Rv3671c - TB.seq 4112322:4113512 MW:40722 >emb|AL123456|MTBH37RV:c4113512-4112319, Rv3671c SEQ ID NO: 136

ATGACCCCGTCGCAGTGGCTGGATATCGCCGTCTTGGCGGTCGCATTTATTGCAGCCATCTCCG GCTGGCGTGCCGGTGCGCTGGGCTCAATGCTGTCGTTTGGCGGGGTGCTGCTGGGCGCGACA

GCCGGCGTGCTGCTGGCGCCGCATATCGTCAGTCAAATCAGCGCTCCGCGGGCCAAACTGTTT GCCGCGCTGTTCCTGATCCTGGCACTGGTCGTAGTCGGCGAGGTCGCTGGTGTGGTGCTGGGC CGCGCCGTCCGCGGGGCGATCCGTAACCGGCCGATCCGGTTGATCGACTCGGTCATTGGGGTA GGGGTGCAGCTGGTCGTGGTGCTCACCGCGGCGTGGTTGTTGGCGATGCCGCTGACACAGTC GAAAGAGCAGCCCGAGCTGGCTGCCGCGGTGAAGGGTTCGCGGGTGCTCGCCCGGGTCAACG AGGCGGCACCCACCTGGCTGAAGACGGTGCCCAAGCGGCTGTCGGCCCTGCTGAACACCTCC GGCCTGCCCGCGGTTTTGGAGCCGTTCAGCCGCACGCCGGTCATTCCAGTGGCCTCACCCGAC

CCAGCGCTGGTCAACAATCCGGTGGTGGCGGCCACCGAGCCAAGTGTCGTCAAAATCCGCAGC CTGGCACCCAGATGCCAGAAAGTGTTGGAGGGCACCGGCTTCGTGATCTCACCCGATCGGGTG ATGACCAACGCGCACGTGGTGGCCGGATCCAACAACGTCACGGTGTATGCCGGCGACAAGCCC TTCGAGGCCACGGTGGTGTCCTACGACCCGTCGGTCGACGTAGCGATCCTGGCCGTTCCGCAC TTGCCGCCGCCGCCGCTGGTCTTCGCTGCGGAGCCGGCGAAAACCGGTGCCGACGTTGTGGT

GCTGGGTTATCCCGGCGGCGGCAATTTCACTGCCACACCCGCCAGGATTCGCGAGGCCATCAG ACTCAGTGGCCCCGATATTTACGGGGACCCGGAGCCGGTTACCCGCGACGTGTACACCATCAG AGCCGATGTGGAGCAAGGTGATTCGGGTGGGCCCCTGATCGACCTCAACGGTCAGGTGCTCGG TGTGGTGTTCGGCGCAGCCATCGACGACGCCGAAACTGGGTTTGTGCTGACGGCCGGCGAGGT GGCGGGGCAGCTTGCCAAAATCGGTGCTACCCAACCGGTCGGCACCGGGGCCTGCGTCAGCT

GA

>Rv3682 ponA2 TB.seq 4121913:4124342 MW:84637 >emb|AL123456|MTBH37RV:4121913-4124345, ponA' SEQ ID NO:137 ATGCCCGAGCGCCTCCCGGCCGCGATCACCGTTCTGAAGCTGGCTGGGTGCTGTCTGTTGGCC

AGTGTCGTCGCCACTGCGCTGACGTTCCCGTTCGCAGGCGGGCTAGGGCTGATGTCCAATCGT GCCTCTGAGGTCGTTGCCAACGGCTCGGCCCAGCTGCTCGAGGGGCAAGTGCCTGCGGTATCG ACGATGGTCGACGCGAAGGGCAACACGATCGCGTGGCTGTACTCGCAGCGCCGGTTCGAGGT GCCCTCGGACAAGATCGCCAACACGATGAAGCTGGCGATCGTCTCGATTGAAGATAAGCGGTTC GCCGACCACAGCGGCGTGGACTGGAAGGGCACCCTGACCGGCCTGGCGGGCTACGCGTCCG

GCGACCTCGACACGCGCGGCGGCTCGACGCTCGAACAACAGTACGTGAAGAACTACCAACTGC TGGTGACAGCCCAAACCGATGCCGAGAAGCGAGCGGCCGTCGAAACCACTCCGGCCCGCAAG CTTCGCGAGATCCGGATGGCACTCACGCTGGACAAGACCTTCACAAAATCTGAAATCCTGACCC GATACTTGAACCTGGTCTCGTTCGGCAATAACTCGTTCGGCGTGCAGGACGCGGCGCAAACGTA CTTCGGCATCAACGCGTCCGACCTGAATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCA

ATCGACCAGCACGCTCAACCCGTACACCAACCCCGACGGCGCGCTGGCCCGGCGGAACGTGG TCCTCGACACCATGATCGAGAACCTTCCCGGGGAGGCGGAGGCGTTGCGTGCCGCCAAGGCC GAGCCGCTGGGGGTACTGCCGCAGCCCAATGAGTTGCCGCGCGGCTGCATCGCGGCCGGCGA CCGCGCATTCTTCTGCGACTACGTCCAGGAGTACCTGTCTCGGGCCGGGATCAGCAAGGAGCA GGTCGCCACGGGCGGGTACCTGATCCGCACCACCCTGGACCCAGAGGTGCAGGCACCGGTCA

AGGCCGCCATCGACAAGTACGCCAGCCCGAACCTGGCCGGTATTTCCAGCGTGATGAGCGTGA TCAAACCGGGTAAGGATGCGCACAAGGTGTTGGCCATGGCCAGTAACCGCAAATACGGGCTGG ATCTAGAAGCCGGCGAAACCATGCGGCCGCAGCCATTCTCCCTGGTTGGCGACGGCGCCGGGT CTATCTTCAAGATCTTCACCACGGCCGCTGCTCTGGACATGGGCATGGGTATTAACGCCCAACT CGACGTGCCGCCCCGATTCCAGGCCAAAGGTCTGGGAAGTGGCGGGGCAAAGGGGTGCCCCA AAGAGACCTGGTGTGTGGTGAACGCCGGCAACTACCGCGGCTCGATGAATGTCACCGACGCGC TGGCAACCTCGCCAAACACCGCGTTCGCCAAGCTGATCTCGCAGGTCGGGGTGGGGCGTGCG

GTCGATATGGCCATCAAACTCGGGCTGAGGTCTTATGCGAATCCCGGCACCGCACGCGACTAC AACCCCGACAGCAATGAGAGCTTGGCTGACTTCGTCAAACGACAGAACCTGGGTTCGTTCACCC TCGGCCCCATCGAGTTAAACGCGCTGGAGCTGTCCAACGTGGCGGCCACGTTGGCATCCGGCG GCGTGTGGTGCCCCCCCAACCCAATCGACCAGCTCATCGACCGCAACGGCAACGAAGTCGCGG TCACCACCGAGACGTGCGACCAGGTGGTGCCCGCAGGGCTGGCGAACACCCTCGCCAACGCG

ATGAGCAAGGACGCCGTGGGCAGCGGCACGGCGGCCGGTTCGGCCGGCGCGGCGGGCTGGG ATCTGCCGATGTCCGGCAAAACCGGCACCACCGAGGCGCACCGGTCGGCCGGCTTCGTGGGC TTCACCAACCGCTACGCGGCGGCGAACTACATCTACGACGACTCCAGCTCGCCGACAGATCTGT GTTCCGGCCCGCTGCGCCATTGCGGCAGCGGCGACTTGTACGGCGGCAACGAGCCATCCCGC ACCTGGTTCGCCGCGATGAAGCCGATCGCCAACAACTTCGGCGAAGTGCAGCTACCACCGACC

GATCCACGCTATGTCGACGGCGCACCAGGCTCACGGGTACCAAGCGTGGCCGGTCTGGATGTC GACGCCGCACGCCAGCGCCTCAAGGACGCGGGCTTCCAGGTCGCCGACCAAACCAACTCGGT CAACAGCTCCGCCAAGTATGGTGAGGTGGTCGGAACGTCGCCCAGCGGTCAAACAATTCCGGG TTCGATCGTCACGATCCAGATCAGCAACGGCATCCCGCCGGCTCCGCCTCCGCCACCGCTGCC TGAGGATGGTGGGCCGCCACCGCCGGTCGGATCGCAGGTGGTGGAGATTCCGGGGCTGCCGC

CGATCACCATTCCGCTGCTGGCGCCACCACCCCCAGCGCCTCCCCCGTAG

>Rv3721c dnaZX DNA polymerase III, [gamma] (dnaZ) and t (dnaX) TB.seq 4164995:4166728 MW:61892 >emb|AL123456|MTBH37RV:c4166728-4164992, dnaZX SEQ ID NO:138 GTGGCTCTCTACCGCAAGTACCGACCGGCAAGCTTCGCGGAGGTGGTGGGGCAGGAGCACGT

CACCGCGCCGCTGTCGGTGGCGCTGGATGCCGGCCGGATCAACCACGCGTACCTGTTCTCTGG GCCGCGTGGCTGCGGAAAGACGTCGTCAGCGCGTATCCTGGCGCGGTCGTTGAACTGTGCGCA GGGCCCTACCGCCAACCCGTGCGGGGTCTGCGAATCCTGCGTTTCGTTGGCGCCCAACGCCCC CGGCAGCATCGACGTGGTAGAGCTGGATGCCGCCAGCCACGGCGGCGTGGACGACACCCGCG AGCTGCGGGACCGCGCGTTCTATGCGCCGGTCCAGTCACGGTACCGGGTATTTATCGTCGACG

AGGCGCACATGGTGACCACCGCGGGATTCAACGCGCTGCTCAAGATCGTGGAGGAACCGCCC GAACACCTGATCTTCATATTCGCCACCACCGAACCGGAGAAGGTACTGCCGACGATTCGGTCGC GCACTCATCACTACCCGTTCCGGCTGCTGCCGCCGCGCACTATGCGGGCGTTGCTCGCGCGGA TCTGCGAGCAGGAGGGCGTCGTCGTCGACGATGCGGTGTACCCGTTGGTGATCCGGGCCGGC GGAGGTTCCCCACGGGATACGCTCTCGGTGCTGGACCAATTGCTGGCTGGGGCCGCGGACAC

CCACGTGACCTACACCCGGGCGCTGGGGCTGCTGGGTGTCACCGACGTCGCCCTGATCGACG ACGCGGTCGACGCACTGGCCGCTTGCGATGCGGCCGCATTGTTCGGGGCGATCGAATCGGTGA TCGATGGCGGACATGACCCTCGGCGTTTCGCTACCGATCTGCTGGAGCGATTCCGCGACCTGA TTGTGCTGCAATCGGTTCCCGACGCGGCATCTCGCGGGGTGGTGGATGCGCCCGAAGACGCG CTGGATCGGATGCGCGAGCAAGCCGCCCGGATCGGGCGGGCGACCCTGACCCGATATGCCGA GGTGGTGCAGGCCGGGCTAGGCGAGATGCGCGGTGCGACCGCGCCGCGTCTGCTGCTGGAA GTGGTTTGCGCGCGACTGCTGCTGCCCTCGGCGAGCGACGCCGAATCGGCACTGTTGCAGCG

GGTCGAACGGATCGAGACCCGGTTGGACATGTCGATCCCGGCGCCGCAAGCCGTACCACGCC CGTCGGCTGCGGCTGCCGAGCCGAAACACCAGCCCGCGCGTGAACCGAGACCGGTGCTGGCC CCCACACCGGCCTCGAGCGAACCCACCGTGGCCGCGGTTCGGTCCATGTGGCCGACGGTGCG CGACAAGGTGCGCCTGCGCAGCCGTACCACCGAGGTGATGCTGGCGGGTGCCACCGTCCGTG CGCTAGAGGACAACACGCTGGTGCTGACCCACGAATCGGCGCCGCTGGCGCGGCGGCTGTCC

GAACAGCGCAACGCCGATGTCCTCGCCGAGGCGCTTAAAGACGCGCTGGGAGTCAACTGGCG GGTGCGGTGTGAGACCGGTGAACCGGCTGCGGCGGCATCACCCGTCGGCGGGGGAGCGAAC GTGGCGACCGCCAAGGCCGTAAACCCTGCCCCCACAGCGAATTCCACTCAGCGCGACGAAGAG GAGCACATGCTCGCCGAAGCCGGCCGTGGCGACCCGTCGCCGCGTCGCGACCCGGAAGAGGT TGCACTCGAGCTGCTGCAGAACGAGCTGGGCGCGCGCCGGATAGACAACGCCTAG

>Rv3783 - TB.seq 4229255:4230094 MW:32337

>emb|AL123456|MTBH37RV:4229255-4230097, Rv3783 SEQ ID NO:139 ATGACATTCATGGATGCTCAAGCTAGCTTCCAGACACAGTCGCGGACACTGGCCCGCGTCCGA GGCGATCTGGTCGACGGGTTCCGCCGCCACGAGCTGTGGCTGCACCTGGGCTGGCAGGACAT

CAAGCAGCGGTACCGCCGCTCGGTGCTGGGGCCGTTCTGGATCACCATCGCCACCGGAACGA CCGCCGTCGCGATGGGCGGCCTGTATTCCAAGCTGTTTCGGCTCGAGCTGTCTGAGCACCTGC CCTACGTCACGCTCGGGCTGATCGTCTGGAACCTGATCAACGCCGCCATCCTGGACGGCGCAG AGGTTTTCGTCGCCAACGAAGGTCTGATCAAACAGCTGCCGGCACCGTTGAGCGTGCACGTCTA TCGGTTGGTGTGGCGGCAGATGATCTTCTTCGCCCACAACATCGTCATCTACTTCGTCATCGCG

ATCATCTTTCCTAAGCCGTGGTCGTGGGCGGATCTGTCGTTTCTTCCGGCGCTGGCGCTCATTT TCCTCAATTGCGTTTGGGTGTCACTGTGTTTCGGCATCCTGGCGACCCGCTACCGCGACATCGG CCCGCTGCTGTTTTCCGTTGTGCAGTTGTTGTTCTTCATGACGCCGATCATCTGGAACGACGAGA CCCTGCGTCGGCAGGGCGCGGGCCGCTGGTCGAGCATCGTCGAGCTCAACCCGCTGCTGCAC TATCTGGACATCGTGCGGGCGCCACTGTTGGGCGCTCACCAGGAGCTGCGGCACTGGCTGGTG

GTGCTGGTGTTGACCGTCGTCGGCTGGATGCTGGCGGCGTTCGCGATGCGGCAGTATCGCGC GCGGGTGCCCTACTGGGTGTAG

>Rv3789 - TB.seq 4235371 :4235733 MW:13378 >emb|AL123456|MTBH37RV:4235371 -4235736, Rv3789 SEQ ID NO:140

ATGCGGTTCGTTGTCACCGGCGGCCTCGCTGGGATAGTTGACTTTGGCCTCTACGTCGTGCTGT ACAAGGTGGCGGGCCTACAGGTCGACCTGTCCAAGGCCATCAGCTTCATCGTCGGCACCATCA CCGCGTACCTGATCAACCGCCGGTGGACATTCCAGGCCGAGCCCAGCACGGCCCGATTCGTCG CGGTCATGCTCCTCTACGGAATCACCTTCGCCGTGCAGGTCGGACTCAACCACCTCTGCCTCGC ACTCTTGCACTACCGGGCGTGGGCCATCCCCGTCGCGTTTGTGATCGCGCAGGGCACCGCCAC GGTAATCAACTTCATCGTGCAGCGAGCCGTGATCTTCCGGATCCGCTGA

>Rv3790 - TB.seq 4235776:4237158 MW:50164

>emb|AL123456|MTBH37RV:4235776-4237161 , Rv3790 SEQ ID NO:141

ATGTTGAGCGTGGGAGCTACCACTACCGCCACCCGGCTGACCGGGTGGGGCCGCACAGCGCC GTCGGTGGCGAATGTGCTTCGCACCCCAGATGCCGAGATGATCGTCAAGGCGGTGGCTCGGGT CGCCGAGTCGGGGGGCGGCCGGGGTGCTATCGCGCGCGGGCTGGGCCGCTCCTATGGGGAC

AACGCCCAAAACGGCGGTGGGTTGGTGATCGACATGACGCCGCTGAACACTATCCACTCCATTG ACGCCGACACCAAGCTGGTCGACATCGACGCCGGGGTCAACCTCGACCAACTGATGAAAGCCG CCCTGCCGTTCGGGCTGTGGGTCCCGGTGCTGCCGGGAACCCGGCAGGTCACCGTCGGCGGG GCGATCGCCTGCGATATCCACGGCAAGAACCATCACAGCGCTGGCAGCTTCGGTAACCACGTG CGCAGCATGGACCTGCTGACCGCCGACGGCGAGATCCGTCATCTCACTCCGACCGGCGAGGA

CGCCGAACTGTTCTGGGCCACCGTCGGGGGCAACGGTCTCACCGGCATCATCATGCGGGCCAC CATCGAGATGACGCCCACTTCGACGGCGTACTTCATCGCCGACGGCGACGTCACCGCCAGCCT CGACGAGACCATCGCCCTGCACAGCGACGGCAGCGAAGCGCGCTACACCTATTCCAGTGCCTG GTTCGACGCGATCAGCGCTCCCCCGAAGCTGGGCCGCGCGGCGGTATCGCGTGGCCGCCTGG CCACCGTCGAGCAATTGCCTGCGAAACTGCGGAGCGAACCTTTGAAATTCGATGCGCCACAGCT

ACTTACGTTGCCCGACGTGTTTCCCAACGGGCTGGCCAACAAATATACCTTCGGCCCGATCGGC GAACTGTGGTACCGCAAATCCGGCACCTATCGCGGCAAGGTCCAGAACCTCACGCAGTTCTACC ATCCGCTGGACATGTTCGGCGAATGGAACCGCGCCTACGGCCCAGCGGGCTTCCTGCAATATC AGTTCGTGATCCCCACAGAGGCGGTTGATGAGTTCAAGAAGATCATCGGCGTTATTCAAGCCTC GGGTCACTACTCGTTTCTCAACGTGTTCAAGCTGTTCGGCCCCCGCAACCAGGCGCCGCTCAGC

TTCCCCATCCCGGGCTGGAACATCTGCGTCGACTTCCCCATCAAGGACGGGCTGGGGAAGTTC GTCAGCGAACTCGACCGCCGGGTACTGGAATTCGGCGGCCGGCTCTACACCGCCAAAGACTCC CGTACCACCGCCGAAACCTTTCATGCCATGTATCCGCGCGTCGACGAATGGATCTCCGTGCGCC GCAAGGTCGATCCGCTGCGCGTATTCGCCTCCGACATGGCCCGACGCTTGGAGCTGCTGTAG

>Rv3791 - TB.seq 4237162:4237923 MW:27470

>emb|AL123456|MTBH37RV:4237162-4237926, Rv3791 SEQ ID NO:142

ATGGTTCTTGATGCCGTAGGAAACCCCCAGACGGTGCTGCTGCTCGGTGGCACCTCCGAGATC GGGCTCGCCATCTGCGAGCGCTACCTGCACAATTCGGCGGCCCGCATCGTGCTGGCCTGCCTG CCCGACGACCCACGGCGGGAGGACGCGGCCGCTGCGATGAAGCAGGCCGGCGCGCGGTCGG

TGGAGCTGATCGACTTTGACGCCCTGGATACCGACAGCCACCCGAAGATGATCGAGGCGGCCT TCTCCGGCGGTGATGTGGACGTGGCTATCGTCGCGTTCGGCTTGCTCGGCGACGCCGAAGAGC TGTGGCAGAACCAGCGCAAGGCGGTGCAGATCGCCGAAATCAACTACACCGCAGCGGTTTCGG TGGGCGTGCTGCTGGCTGAGAAGATGCGCGCTCAGGGCTTCGGTCAGATCATCGCGATGAGCT CGGCCGCCGGTGAGCGGGTGCGACGGGCGAACTTCGTCTACGGCTCCACCAAGGCCGGTCTG GACGGGTTTTACCTGGGGTTGTCAGAAGCGCTGCGCGAGTACGGTGTTCGTGTGCTGGTGATC CGGCCCGGCCAGGTGCGTACCCGGATGAGCGCGCACCTCAAGGAAGCTCCATTGACCGTCGA

CAAGGAGTACGTCGCCAACCTCGCGGTGACCGCGTCCGCAAAAGGTAAGGAATTGGTTTGGGC GCCAGCAGCGTTCCGCTACGTCATGATGGTGTTGCGTCACATCCCGCGGAGCATCTTCCGCAA GCTGCCCATCTGA

>Rv3794 embA TB.seq 4243230:4246511 MW: 115694

>emb|AL123456|MTBH37RV:4243230-4246514, embA SEQ ID NO:143

GTGCCCCACGACGGTAATGAGCGATCTCACCGGATCGCACGCCTAGCAGCCGTCGTCTCGGGA ATCGCGGGTCTGCTGCTGTGCGGCATCGTTCCGCTGCTTCCGGTGAACCAAACCACCGCGACC ATCTTCTGGCCGCAGGGCAGCACCGCCGACGGCAACATCACCCAGATCACCGCCCCTCTGGTA TCCGGGGCGCCACGCGCGCTGGACATCTCGATCCCCTGCTCGGCCATCGCCACGCTGCCCGC

CAACGGCGGCCTGGTGCTGTCCACACTGCCGGCCGGTGGCGTGGATACCGGTAAGGCCGGGC TGTTCGTCCGCGCCAACCAGGACACGGTCGTCGTGGCGTTCCGCGACTCGGTGGCCGCGGTG GCGGCCCGCTCCACGATCGCAGCGGGAGGCTGTAGCGCGCTGCATATCTGGGCCGATACCGG CGGCGCGGGCGCTGATTTTATGGGTATACCCGGCGGCGCCGGGACCCTGCCGCCGGAGAAGA AGCCACAGGTTGGCGGCATCTTCACCGACCTGAAGGTCGGAGCGCAGCCCGGGCTGTCGGCC

CGCGTCGACATCGACACTCGGTTTATCACGACGCCCGGCGCGCTCAAGAAGGCCGTGATGCTC CTCGGCGTGCTGGCGGTCCTGGTAGCCATGGTGGGGCTGGCCGCGCTGGACCGGCTCAGCAG GGGCCGCACCCTGCGCGACTGGCTGACCCGATATCGCCCGCGGGTGCGGGTCGGATTCGCCA GCCGGCTCGCTGACGCAGCGGTGATCGCGACCTTGTTGCTCTGGCATGTCATCGGCGCCACCT CGTCCGATGACGGCTACCTTCTGACCGTCGCCCGGGTCGCCCCGAAGGCCGGCTATGTAGCCA

ACTACTACCGGTATTTCGGCACGACGGAGGCGCCGTTCGACTGGTATACATCGGTGCTTGCCCA GCTGGCGGCGGTGAGCACCGCCGGCGTCTGGATGCGCCTGCCCGCCACCCTGGCCGGAATCG CCTGCTGGCTGATCGTCAGCCGTTTCGTGCTGCGGCGGCTGGGACCGGGCCCGGGCGGGCTG GCGTCCAACCGGGTCGCTGTGTTCACCGCTGGTGCGGTGTTCCTGTCCGCCTGGCTGCCGTTC AACAACGGCCTGCGTCCCGAGCCGCTGATCGCGCTGGGTGTGCTGGTCACGTGGGTGTTGGTG

GAACGGTCGATCGCGCTCGGACGGCTGGCCCCGGCCGCGGTAGCCATCATCGTGGCGACGCT TACCGCGACGCTGGCACCGCAGGGGTTGATCGCGCTGGCCCCGCTGCTGACTGGTGCGCGCG CCATCGCCCAGAGGATCCGGCGCCGCCGGGCGACCGATGGACTGCTGGCGCCGCTGGCGGT GCTGGCCGCGGCGTTGTCGCTGATCACCGTGGTGGTGTTTCGGGACCAGACGCTGGCCACGGT GGCCGAATCGGCACGCATCAAGTACAAGGTCGGCCCGACCATCGCCTGGTACCAGGACTTCCT

GCGCTACTACTTCCTTACCGTGGAGAGCAACGTTGAGGGGTCGATGTCCCGCCGGTTCGCGGT GCTGGTGTTGCTGTTCTGCCTGTTCGGGGTGCTGTTCGTGCTGCTGCGGCGCGGCCGGGTGGC GGGGCTGGCCAGCGGCCCGGCCTGGCGACTGATCGGCACTACGGCGGTCGGCCTGCTGCTGC TCACGTTCACGCCAACCAAGTGGGCCGTGCAGTTCGGCGCATTCGCCGGGCTGGCCGGGGTGT TGGGTGCGGTCACCGCGTTCACCTTTGCCCGCATCGGTCTACATAGTCGACGCAACCTCACGCT GTACGTGACCGCGTTGCTGTTCGTGCTGGCGTGGGCAACCTCGGGCATCAACGGGTGGTTCTA CGTCGGCAACTACGGGGTGCCGTGGTATGACATCCAGCCCGTCATCGCCAGCCACCCGGTGAC

GTCGATGTTTCTGACGCTGTCGATCCTCACCGGATTGCTGGCAGCCTGGTATCACTTCCGGATG GACTACGCCGGGCACACCGAAGTCAAAGACAACCGGCGCAACCGCATCTTGGCCTCTACGCCA CTGCTGGTGGTCGCGGTGATCATGGTCGCAGGCGAAGTCGGCTCGATGGCCAAGGCCGCGGT GTTCCGTTACCCGCTTTACACCACCGCCAAGGCCAACCTGACCGCGCTCAGCACCGGGCTGTC CAGCTGTGCGATGGCCGACGACGTGCTGGCCGAGCCCGACCCCAATGCCGGCATGCTGCAAC

CGGTTCCGGGCCAGGCGTTCGGACCGGACGGACCGCTGGGCGGTATCAGTCCCGTCGGCTTC AAACCCGAGGGCGTGGGCGAGGACCTCAAGTCCGACCCGGTGGTCTCCAAACCCGGGCTGGT CAACTCCGATGCGTCGCCCAACAAACCCAACGCCGCCATCACCGACTCCGCGGGCACCGCCGG AGGGAAGGGCCCGGTCGGGATCAACGGGTCGCACGCGGCGCTGCCGTTCGGATTGGACCCGG CACGTACCCCGGTGATGGGCAGCTACGGGGAGAACAACCTGGCCGCCACGGCCACCTCGGCC

TGGTACCAGTTACCGCCCCGCAGCCCGGACCGGCCGCTGGTGGTGGTTTCCGCGGCCGGCGC CATCTGGTCCTACAAGGAGGACGGCGATTTCATCTACGGCCAGTCCCTGAAACTGCAGTGGGG CGTCACCGGCCCGGACGGCCGCATCCAGCCACTGGGGCAGGTATTTCCGATCGACATCGGACC GCAACCCGCGTGGCGCAATCTGCGGTTTCCGCTGGCCTGGGCGCCGCCGGAGGCCGACGTGG CGCGCATTGTCGCCTATGACCCGAACCTGAGCCCTGAGCAATGGTTCGCCTTCACCCCGCCCC

GGGTTCCGGTGCTGGAATCTCTGCAGCGGTTGATCGGGTCAGCGACACCGGTGTTGATGGACA TCGCGACCGCAGCCAACTTCCCCTGCCAGCGACCGTTTTCCGAGCATCTCGGCATTGCCGAGC TTCCGCAGTACCGGATCCTGCCGGACCACAAGCAGACGGCGGCGTCGTCGAACCTATGGCAGT CCAGCTCGACCGGCGGTCCGTTCCTGTTCACCCAGGCGCTGCTGCGCACCTCGACGATCGCCA CGTACCTGCGTGGGGACTGGTATCGCGACTGGGGATCGGTGGAGCAGTACCACCGGCTGGTG

CCGGCCGATCAGGCTCCAGACGCCGTTGTCGAGGAGGGCGTGATCACTGTGCCCGGCTGGGG TCGGCCAGGACCGATCAGGGCGCTGCCATGA

>Rv3795 embB TB.seq 4246511 :4249804 MW: 118023 >emb|AL123456|MTBH37RV:4246511-4249807, embB SEQ ID NO:144

ATGACACAGTGCGCGAGCAGACGCAAAAGCACCCCAAATCGGGCGATTTTGGGGGCTTTTGCG TCTGCTCGCGGGACGCGCTGGGTGGCCACCATCGCCGGGCTGATTGGCTTTGTGTTGTCGGTG GCGACGCCGCTGCTGCCCGTCGTGCAGACCACCGCGATGCTCGACTGGCCACAGCGGGGGCA ACTGGGCAGCGTGACCGCCCCGCTGATCTCGCTGACGCCGGTCGACTTTACCGCCACCGTGCC GTGCGACGTGGTGCGCGCCATGCCACCCGCGGGCGGGGTGGTGCTGGGCACCGCACCCAAG

CAAGGCAAGGACGCCAATTTGCAGGCGTTGTTCGTCGTCGTCAGCGCCCAGCGCGTGGACGTC ACCGACCGCAACGTGGTGATCTTGTCCGTGCCGCGCGAGCAGGTGACGTCCCCGCAGTGTCAA CGCATCGAGGTCACCTCTACCCACGCCGGCACCTTCGCCAACTTCGTCGGGCTCAAGGACCCG TCGGGCGCGCCGCTGCGCAGCGGCTTCCCCGACCCCAACCTGCGCCCGCAGATTGTCGGGGT GTTCACCGACCTGACCGGGCCCGCGCCGCCCGGGCTGGCGGTCTCGGCGACCATCGACACCC GGTTCTCCACCCGGCCGACCACGCTGAAACTGCTGGCGATCATCGGGGCGATCGTGGCCACCG TCGTCGCACTGATCGCGTTGTGGCGCCTGGACCAGTTGGACGGGCGGGGCTCAATTGCCCAGC

TCCTCCTCAGGCCGTTCCGGCCTGCATCGTCGCCGGGCGGCATGCGCCGGCTGATTCCGGCAA GCTGGCGCACCTTCACCCTGACCGACGCCGTGGTGATATTCGGCTTCCTGCTCTGGCATGTCAT CGGCGCGAATTCGTCGGACGACGGCTACATCCTGGGCATGGCCCGAGTCGCCGACCACGCCG GCTACATGTCCAACTATTTCCGCTGGTTCGGCAGCCCGGAGGATCCCTTCGGCTGGTATTACAA CCTGCTGGCGCTGATGACCCATGTCAGCGACGCCAGTCTGTGGATGCGCCTGCCAGACCTGGC

CGCCGGGCTAGTGTGCTGGCTGCTGCTGTCGCGTGAGGTGCTGCCCCGCCTCGGGCCGGCGG TGGAGGCCAGCAAACCCGCCTACTGGGCGGCGGCCATGGTCTTGCTGACCGCGTGGATGCCG TTCAACAACGGCCTGCGGCCGGAGGGCATCATCGCGCTCGGCTCGCTGGTCACCTATGTGCTG ATCGAGCGGTCCATGCGGTACAGCCGGCTCACACCGGCGGCGCTGGCCGTCGTTACCGCCGC ATTCACACTGGGTGTGCAGCCCACCGGCCTGATCGCGGTGGCCGCGCTGGTGGCCGGCGGCC

GCCCGATGCTGCGGATCTTGGTGCGCCGTCATCGCCTGGTCGGCACGTTGCCGTTGGTGTCGC CGATGCTGGCCGCCGGCACCGTCATCCTGACCGTGGTGTTCGCCGACCAGACCCTGTCAACGG TGTTGGAAGCCACCAGGGTTCGCGCCAAAATCGGGCCGAGCCAGGCGTGGTATACCGAGAACC TGCGTTACTACTACCTCATCCTGCCCACCGTCGACGGTTCGCTGTCGCGGCGCTTCGGCTTTTT GATCACCGCGCTATGCCTGTTCACCGCGGTGTTCATCATGTTGCGGCGCAAGCGAATTCCCAGC

GTGGCCCGCGGACCGGCGTGGCGGCTGATGGGCGTCATCTTCGGCACCATGTTCTTCCTGATG TTCACGCCCACCAAGTGGGTGCACCACTTCGGGCTGTTCGCCGCCGTAGGGGCGGCGATGGC CGCGCTGACGACGGTGTTGGTATCCCCATCGGTGCTGCGCTGGTCGCGCAACCGGATGGCGTT CCTGGCGGCGTTATTCTTCCTGCTGGCGTTGTGTTGGGCCACCACCAACGGCTGGTGGTATGTC TCCAGCTACGGTGTGCCGTTCAACAGCGCGATGCCGAAGATCGACGGGATCACAGTCAGCACA

ATCTTTTTCGCCCTGTTTGCGATCGCCGCCGGCTATGCGGCCTGGCTGCACTTCGCGCCCCGC GGCGCCGGCGAAGGGCGGCTGATCCGCGCGCTGACGACAGCCCCGGTACCGATCGTGGCCG GTTTCATGGCGGCGGTGTTCGTCGCGTCCATGGTGGCCGGGATCGTGCGACAGTACCCGACCT ACTCCAACGGCTGGTCCAACGTGCGGGCGTTTGTCGGCGGCTGCGGACTGGCCGACGACGTA CTCGTCGAGCCTGATACCAATGCGGGTTTCATGAAGCCGCTGGACGGCGATTCGGGTTCTTGG

GGCCCCTTGGGCCCGCTGGGTGGAGTCAACCCGGTCGGCTTCACGCCCAACGGCGTACCGGA ACACACGGTGGCCGAGGCGATCGTGATGAAACCCAACCAGCCCGGCACCGACTACGACTGGGA TGCGCCGACCAAGCTGACGAGTCCTGGCATCAATGGTTCTACGGTGCCGCTGCCCTATGGGCT CGATCCCGCCCGGGTACCGTTGGCAGGCACCTACACCACCGGCGCACAGCAACAGAGCACACT CGTCTCGGCGTGGTATCTCCTGCCTAAGCCGGACGACGGGCATCCGCTGGTCGTGGTGACCGC

CGCGGGCAAGATCGCCGGCAACAGCGTGCTGCACGGGTACACCCCCGGGCAGACTGTGGTGC TCGAATACGCCATGCCGGGACCCGGAGCGCTGGTACCCGCCGGGCGGATGGTGCCCGACGAC CTATACGGAGAGCAGCCCAAGGCGTGGCGCAACCTGCGCTTCGCCCGAGCAAAGATGCCCGC CGATGCCGTCGCGGTCCGGGTGGTGGCCGAGGATCTGTCGCTGACACCGGAGGACTGGATCG CGGTGACCCCGCCGCGGGTACCGGACCTGCGCTCACTGCAGGAATATGTGGGCTCGACGCAG CCGGTGCTGCTGGACTGGGCGGTCGGTTTGGCCTTCCCGTGCCAGCAGCCGATGCTGCACGC CAATGGCATCGCCGAAATCCCGAAGTTCCGCATCACACCGGACTACTCGGCTAAGAAGCTGGAC

ACCGACACGTGGGAAGACGGCACTAACGGCGGCCTGCTCGGGATCACCGACCTGTTGCTGCG GGCCCACGTCATGGCCACCTACCTGTCCCGCGACTGGGCCCGCGATTGGGGTTCCCTGCGCAA GTTCGACACCCTGGTCGATGCCCCTCCCGCCCAGCTCGAGTTGGGCACCGCGACCCGCAGCG GCCTGTGGTCACCGGGCAAGATCCGAATTGGTCCATAG

>Rv3834c serS seryl-tRNA synthase TB.seq 4307655:4308911 MW:45293 >emb|AL123456|MTBH37RV:c4308911-4307652, serS SEQ ID NO: 145

GTGATCGACCTGAAGCTGCTTCGTGAAAACCCCGACGCGGTACGCCGCTCACAACTCAGCCGC GGCGAGGACCCGGCGCTGGTAGATGCCCTGCTGACGGCCGACGCCGCCCGCCGGGCCGTGA TCTCG ACCGCCG ATTCGTTACGGGCCGAGCAG AAAGCCGCCAGCAAAAGCGTGGGTGGCGCG

TCTCCCGAAGAGCGCCCGCCGCTGCTGCGGCGCGCGAAGGAACTCGCCGAGCAGGTCAAAGC CGCTGAGGCCGACGAGGTCGAAGCGGAGGCGGCGTTCACCGCGGCGCACCTGGCGATCTCGA ATGTCATCGTGGACGGGGTACCCGCCGGCGGGGAGGACGACTACGCGGTGCTCGACGTCGTC GGCGAGCCCAGCTACCTCGAGAACCCCAAGGACCACCTGGAGCTCGGCGAGTCGCTGGGCCT GATCGACATGCAGCGCGGCGCCAAGGTGTCGGGTTCACGGTTCTACTTCCTGACCGGTCGGGG

TGCCCTACTGCAGCTTGGATTGCTGCAGCTGGCGCTGAAGCTAGCCGTCGACAACGGCTTTGTC CCTACGATCCCGCCGGTGCTGGTGCGCCCGGAAGTGATGGTAGGCACGGGATTTCTAGGCGCC CACGCCGAGGAGGTGTACCGGGTAGAGGGCGACGGCCTCTACCTTGTGGGCACCTCCGAGGT ACCGCTGGCGGGGTATCACTCCGGCGAGATTCTGGACCTTTCCCGCGGGCCGCTGCGGTATGC GGGCTGGTCGTCGTGTTTCCGACGTGAGGCCGGCAGCCATGGCAAGGACACGCGCGGCATCA

TCCGGGTGCACCAGTTCGACAAAGTCGAGGGCTTCGTCTACTGCACACCGGCCGACGCGGAGC ACGAACATGAGCGGCTGCTGGGCTGGCAGCGCCAGATGCTGGCACGCATCGAGGTGCCGTAT CGGGTCATCGACGTGGCCGCGGGTGATCTCGGCTCGTCGGCCGCCCGCAAGTTCGACTGCGA GGCGTGGATTCCGACGCAGGGGGCCTATCGCGAGCTGACGTCGACGTCGAACTGCACCACCTT TCAGGCGCGCCGGTTGGCGACCCGCTACCGGGATGCCAGCGGCAAGCCGCAGATCGCGGCCA

CCCTCAACGGAACGCTGGCCACCACCCGGTGGCTGGTTGCGATCCTGGAGAACCACCAGCGG CCCGACGGCAGCGTTAGAGTCCCGGACGCACTGGTTCCGTTCGTGGGTGTCGAAGTGCTGGAG CCGGTCGCTTAG

>Rv3907c pcnA polynucleotide polymerase TB.seq 4391631 :4393070 MW:53057

>emb|AL123456|MTBH37RV:c4393070-4391628, pcnA SEQ ID NO:146 GTGCCGGAAGCCGTCCAGGAAGCCGATCTGCTAACCGCCGCTGCGGTTGCCTTGAACAGGCAT GCTGCCTTATTGCGGGAACTCGGGTCGGTGTTCGCCGCCGCGGGACACGAGTTGTATCTGGTC GGCGGTTCGGTGCGAGATGCACTGTTGGGCCGGTTGAGCCCCGACCTGGACTTCACCACCGAC GCCCGTCCCGAGCGGGTGCAGGAGATCGTGCGGCCGTGGGCCGATGCGGTGTGGGATACCG GAATCGAATTCGGCACCGTCGGCGTGGGTAAGAGCGACCACCGCATGGAGATCACCACATTCC

GTGCCGACAGCTACGACCGGGTTTCGCGTCATCCAGAGGTACGTTTCGGCGATTGCCTCGAGG GCGATCTGGTCCGCCGCGACTTCACCACGAACGCAATGGCTGTGCGCGTCACCGCCACTGGGC CGGGCGAATTCCTGGATCCGCTTGGTGGCTTGGCGGCGCTGCGGGCCAAGGTGTTAGACACCC CGGCGGCGCCGTCGGGGTCCTTTGGCGACGATCCGTTGCGGATGCTGCGCGCCGCGCGGTTC GTCTCGCAACTTGGATTCGCGGTGGCGCCGCGGGTGCGCGCGGCGATCGAAGAGATGGCGCC

GCAGTTGGCCCGAATCAGCGCCGAACGGGTGGCCGCCGAGCTGGACAAGCTGCTGGTCGGTG AGGATCCGGCCGCGGGTATCGACCTGATGGTGCAGAGCGGTATGGGTGCTGTGGTCTTGCCTG AAATCGGTGGGATGCGGATGGCGATCGACGAACATCACCAGCACAAGGACGTCTATCAGCATTC CTTGACCGTGCTGCGGCAGGCGATCGCGCTGGAGGACGACGGCCCGGATCTGGTGTTGCGCT GGGCGGCGCTGCTGCACGACATCGGCAAGCCCGCCACCCGCCGTCACGAACCCGACGGTGGG

GTGAGCTTCCATCACCACGAAGTGGTCGGCGCCAAGATGGTGCGCAAGCGGATGCGGGCGCT GAAGTATTCCAAGCAGATGATCGACGACATCTCGCAGCTGGTCTACCTGCATCTGCGGTTTCAC GGCTACGGCGATGGGAAATGGACCGACTCTGCGGTGCGCCGCTATGTCACCGACGCCGGGGC CCTACTGCCACGGCTGCACAAGCTGGTGCGCGCCGACTGCACGACCCGCAACAAGCGCCGGG CCGCGCGGTTGCAGGCCAGTTACGACCGGCTGGAAGAGCGGATCGCGGAGCTGGCCGCCCAG

GAGGATCTGGATCGGGTGCGCCCCGACCTGGACGGCAACCAGATCATGGCGGTGCTCGACATT CCGGCGGGCCCGCAAGTCGGCGAGGCGTGGCGCTACTTGAAGGAGCTGCGGCTAGAGCGCG GCCCGTTGTCCACCGAGGAGGCGACAACCGAGCTGCTGTCCTGGTGGAAATCACGGGGGAAC CGCTAG

TABLE 4 >Rv0002 dnaN DNA polymerase III, b-subunit TB.seq 2052:3257 MW:42114 SEQ ID NO:147

MDAATTRVGLTDLTFRLLRESFADAVSWVAKNLPARPAVPVLSGVLLTGSDNGLTISGFDYEVSAEA QVGAEIVSPGSVLVSGRLLSDITRALPNKPVDVHVEGNRVALTCGNARFSLPTMPVEDYPTLPTLPEE TGLLPAELFAEAISQVAIAAGRDDTLPMLTGIRVEILGETWLAATDRFRLAVRELKWSASSPDIEAAVL VPAKTLAEAAKAGIGGSDVRLSLGTGPGVGKDGLLGISGNGKRSTTRLLDAEFPKFRQLLPTEHTAVA TMDVAELIEAIKLVALVADRGAQVRMEFADGSVRLSAGADDVGRAEEDLWDYAGEPLTIAFNPTYLT DGLSSLRSERVSFGFTTAGKPALLRPVSGDDRPVAGLNGNGPFPAVSTDYVYLLMPVRLPG

>Rv0003 recF DNA replication and SOS induction TB.seq 3280:4434 MW.42181 SEQ ID NO: 148

VYVRHLGLRDFRSWACVDLELHPGRTVFVGPNGYGKTNLIEALWYSTTLGSHRVSADLPLIRVGTDR AVISTIWNDGRECAVDLEIATGRVNKARLNRSSVRSTRDWGVLRAVLFAPEDLGLVRGDPADRRR YLDDLAIVRRPAIAAVRAEYERVLRQRTALLKSVPGARYRGDRGVFDTLEVWDSRLAEHGAELVAARI DLVNQLAPEVKKAYQLLAPESRSASIGYRASMDVTGPSEQSDIDRQLLAARLLAALAARRDAELERG VCLVGPHRDDLILRLGD^QPAKGFASHGEAWSLAVALRLAAYQLLRVDGGEPVLLLDDVFAELDVMRR RALATAAESAEQVLVTAAVLEDIPAGWDARRVHIDVRADDTGSMSWLP

>Rv0005 gyrB DNA gyrase subunit B TB.seq 5123:7264 MW:78441 SEQ ID NO: 149

MGKNEARRSALAPDHGTWCDPLRRLNRMHATPEESIRIVAAQKKKAQDEYGAASITILEGLEAVRKR PGMYIGSTGERGLHHLIWEWDNAVDEAMAGYATTVNWLLEDGGVEVADDGRGIPVATHASGIPTV DWMTQLHAGGKFDSDAYAISGGLHGVGVSWNALSTRLEVEIKRDGYEWSQVYEKSEPLGLKQGA PTKKTGSTVRFWADPAVFETTEYDFETVARRLQEMAFLNKGLTINLTDERVTQDEN/VDEWSDVAEA PKSASERAAESTAPHKVKSRTFHYPGGLVDFVKHINRTKNAIHSSIVDFSGKGTGHEVEIAMQWNAG YSESVHTFANTINTHEGGTHEEGFRSALTSWNKYAKDRKLLKDKDPNLTGDDIREGLAAVISVKVSE PQFEGQTKTKLGNTEVKSFVQKVCNEQLTHWFEANPTDAKVWNKAVSSAQARIAARKARELVRRK SATDIGGLPGKLADCRSTDPRKSELYWEGDSAGGSAKSGRDSMFQAILPLRGKIINVEKARIDRVLK NTEVQAIITALGTGIHDEFDIGKLRYHKIVLMADADVDGQHISTLLLTLLFRFMRPLIENGHVFLAQPPLY KLKWQRSDPEFAYSDRERDGLLEAGLKAGKKINKEDGIQRYKGLGEMDAKELWETTMDPSVRVLRQ VTLDDAAAADELFSILMGEDVDARRSFITRNAKDVRFLDV

>Rv0006 gyrA DNA gyrase subunit A TB.seq 7302:9815 MW:92276 SEQ ID NO:150 MTDTTLPPDDSLDRIEPVDIEQEMQRSYIDYAMSVIVGRALPEVRDGLKPVHRRVLYAMFDSGFRPD RSHAKSARSVAETMGNYHPHGDASIYDSLVRMAQPWSLRYPLVDGQGNFGSPGNDPPAAMRYTEA RLTPLAMEMLREIDEETVDFIPNYDGRVQEPTVLPSRFPNLLANGSGGIAVGMATNIPPHNLRELADA VFWALENHDADEEETLAAVMGRVKGPDFPTAGLIVGSQGTADAYKTGRGSIRMRGWEVEEDSRG RTSLVITELPYQVNHDNFITSIAEQVRDGKLAGISNIEDQSSDRVGLRIVIEIKRDAVAKWINNLYKHTQ LQTSFGANMLAIVDGVPRTLRLDQLIRYYVDHQLDVIVRRTTYRLRKANERAHILRGLVKALDALDEVI ALIRASETVDIARAGLIELLDIDEIQAQAILDMQLRRLAALERQRIIDDLAKIEAEIADLEDILAKPERQRGI VRDELAEIVDRHGDDRRTRIIAADGDVSDEDLIAREDVWTITETGYAKRTKTDLYRSQKRGGKGVQG AGLKQDDIVAHFFVCSTHDLILFFTTQGRVYRAKAYDLPEASRTARGQHVANLLAFQPEERIAQVIQIR GYTDAPYLVLATRNGLVKKSKLTDFDSNRSGGIVAVNLRDNDELVGAVLCSAGDDLLLVSANGQSIR FSATDEALRPMGRATSGVQGMRFNIDDRLLSLNWREGTYLLVATSGGYAKRTAIEEYPVQGRGGK GVLTVMYDRRRGRLVGALIVDDDSELYAVTSGGGVIRTAARQVRKAGRQTKGVRLMNLGEGDTLLAI ARNAEESGDDNAVDANGADQTGN

>Rv0014c pknB serine-threonine protein kinase TB.seq 15593:17470 MW:66511 SEQ ID NO:151 MTTPSHLSDRYELGEILGFGGMSEVHLARDLRLHRDVAVKVLRADLARDPSFYLRFRREAQNAAALN

HPAIVAVYDTGEAETPAGPLPYIVMEYVDGVTLRDIVHTEGPMTPKRAIEVIADACQALNFSHQNGIIH RDVKPANIMISATNAVKVMDFGIARAIADSGNSVTQTAAVIGTAQYLSPEQARGDSVDARSDVYSLGC VLYEVLTGEPPFTGDSPVSVAYQHVREDPIPPSARHEGLSADLDAWLKALAKNPENRYQTAAEMRA DLVRVHNGEPPEAPKVLTDAERTSLLSSAAGNLSGPRTDPLPRQDLDDTDRDRSIGSVGRWVAWA VLAVLTVWTIAINTFGGITRDVQVPDVRGQSSADAIATLQNRGFKIRTLQKPDSTIPPDHVIGTDPAAN TSVSAGDEITVNVSTGPEQREIPDVSTLTYAEAVKKLTAAGFGRFKQANSPSTPELVGKVIGTNPPAN QTSAITNWIIIVGSGPATKDIPDVAGQTVDVAQKNLNVYGFTKFSQASVDSPRPAGEVTGTNPPAGT TVPVDSVIELQVSKGNQFVMPDLSGMFWVDAEPRLRALGWTGMLDKGADVDAGGSQHNRWYQN PPAGTGVNRDGIITLRFGQ

>Rv0016c pbpA TB.seq 18762:20234 MW:51577 SEQ ID NO: 152 MNASLRRISVTVMALIVLLLLNATMTQVFTADGLRADPRNQRVLLDEYSRQRGQITAGGQLLAYSYAT DGRFRFLRVYPNPEVYAPVTGFYSLRYSSTALERAEDPILNGSDRRLFGRRLADFFTGRDPRGGNV DTTINPRIQQAGWDAMQQGCYGPCKGAWALEPSTGKILALVSSPSYDPNLLASHNPEVQAQAWQR LGDNPASPLTNRAISETYPPGSTFKVITTAAALAAGATETEQLTAAPTIPLPGSTAQLENYGGAPCGDE PTVSLREAFVKSCNTAFVQLGIRTGADALRSMARAFGLDSPPRPTPLQVAESTVGPIPDSAALGMTSI GQKDVALTPLANAEIAATIANGGITMRPYLVGSLKGPDLANISTTVGYQQRRAVSPQVAAKLTELMVG AEKVAQQKGAIPGVQIASKTGTAEHGTDPRHTPPHAWYIAFAPAQAPKVAVAVLVENGADRLSATGG ALAAPIGRAVIEAALQGEP

>Rv0017c rodA TB.seq 20234:21640 MW:50612 SEQ ID NO: 153 MTTRLQAPVAVTPPLPTRRNAELLLLCFAAVITFAALLWQANQDQGVPWDLTSYGLAFLTLFGSAHL AIRRFAPYTDPLLLPWALLNGLGLVMIHRLDLVDNEIGEHRHPSANQQMLWTLVGVAAFALWTFLK DHRQLARYGYICGLAGLVFLAVPALLPAALSEQNGAKIWIRLPGFSIQPAEFSKILLLIFFSAVLVAKRG LFTSAGKHLLGMTLPRPRDLAPLLAAWVISVGVMVFEKDLGASLLLYTSFLWVYLATQRFSWWIGL TLFAAGTLVAYFIFEHVRLRVQTWLDPFADPDGTGYQIVQSLFSFATGGIFGTGLGNGQPDTVPAAST DFIIAAFGEELGLVGLTAILMLYTIVIIRGLRTAIATRDSFGKLLAAGLSSTLAIQLFIWGGVTRLIPLTGLT TPWMSYGGSSLLANYILLAILARISHGARRPLRTRPRNKSPITAAGTEVIERV

>Rv0018c ppp TB.seq 21640:23181 MW:53781 SEQ ID NO:154

VARVTLVLRYAARSDRGLVRANNEDSVYAGARLLALADGMGGHAAGEVASQLVIAALAHLDDDEPG GDLLAKLDAAVRAGNSAIAAQVEMEPDLEGMGTTLTAILFAGNRLGLVHIGDSRGYLLRDGELTQITK DDTFVQTLVDEGRITPEEAHSHPQRSLIMRALTGHEVEPTLTMREARAGDRYLLCSDGLSDPVSDETI LEALQIPEVAESAHRLIELALRGGGPDNVTVWADWDYDYGQTQPILAGAVSGDDDQLTLPNTAAG RASAISQRKEIVKRVPPQADTFSRPRWSGRRLAFWALVTVLMTAGLLIGRAIIRSNYYVADYAGSVSI MRGIQGSLLGMSLHQPYLMGCLSPRNELSQISYGQSGGPLDCHLMKLEDLRPPERAQVRAGLPAGT LDDAIGQLRELAANSLLPPCPAPRATSPPGRPAPPTTSETTEPNVTSSPASPSPTTSAPAPTGTTPAIP TSASPAAPASPPTPWPVTSSPTMAALPPPPPQPGIDCRAAA >Rv0019c - TB.seq 23273:23737 MW:17153 SEQ ID NO:155

MQGLVLQLTRAGFLMLLWVFIWSVLRILKTDIYAPTGAVMMRRGLALRGTLLGARQRRHAARYLWT EGALTGARITLSEQPVLIGRADDSTLVLTDDYASTRHARLSMRGSEWYVEDLGSTNGTYLDRAKVTT AVRVPIGTPVRIGKTAIELRP

>Rv0020c - TB.seq 23864:25444 MW:56881 SEQ ID NO: 156

MGSQKRLVQRVERKLEQTVGDAFARIFGGSIVPQEVEALLRREAADGIQSLQGNRLLAPNEYIITLGV HDFEKLGADPELKSTGFARDLADYIQEQGWQTYGDVWRFEQSSNLHTGQFRARGTVNPDVETHP PVIDCARPQSNHAFGAEPGVAPMSDNSSYRGGQGQGRPDEYYDDRYARPQEDPRGGPDPQGGS DPRGGYPPETGGYPPQPGYPRPRHPDQGDYPEQIGYPDQGGYPEQRGYPEQRGYPDQRGYQDQ GRGYPDQGQGGYPPPYEQRPPVSPGPAAGYGAPGYDQGYRQSGGYGPSPGGGQPGYGGYGEY GRGPARHEEGSYVPSGPPGPPEQRPAYPDQGGYDQGYQQGATTYGRQDYGGGADYTRYTESPR VPGYAPQGGGYAEPAGRDYDYGQSGAPDYGQPAPGGYSGYGQGGYGSAGTSVTLQLDDGSGRT YQLREGSNIIGRGQDAQFRLPDTGVSRRHLEIRWDGQVALLADLNSTNGTTVNNAPVQEWQLADGD VIRLGHSEIIVRMH

>Rv0032 bioF2 C-terminal similar to B. subtilis BioF TB.seq 34295:36607 MW:86245 SEQ ID NO:157

MPTGLGYDFLRPVEDSGINDLKHYYFMADLADGQPLGRANLYSVCFDLATTDRKLTPAWRTTIKRWF PGFMTFRFLECGLLTMVSNPLALRSDTDLERVLPVLAGQMDQLAHDDGSDFLMIRDVDPEHYQRYL DILRPLGFRPALGFSRVDTTISWSSVEEALGCLSHKRRLPLKTSLEFRERFGIEVEELDEYAEHAPVLA RLWRNVKTEAKDYQREDLNPEFFAACSRHLHGRSRLWLFRYQGTPIAFFLNVWGADENYILLEWGI DRDFEHYRKANLYRAALMLSLKDAISRDKRRMEMGITNYFTKLRIPGARVIPTIYFLRHSTDPVHTATL ARMMMHNIQRPTLPDDMSEEFCRWEERIRLDQDGLPEHDIFRKIDRQHKYTGLKLGGVYGFYPRFT GPQRSTVKAAELGEIVLLGTNSYLGLATHPEWEASAEATRRYGTGCSGSPLLNGTLDLHVSLEQEL ACFLGKPAAVLCSTGYQSNLAAISALCESGDMIIQDALNHRSLFDAARLSGADFTLYRHNDMDHLARV LRRTEGRRRIIWDAVFSMEGTVADLATIAELADRHGCRVYVDESHALGVLGPDGRGASAALGVLAR MDWMGTFSKSFASVGGFIAGDRPWDYIRHNGSGHVFSASLPPAAAAATHAALRVSRREPDRRAR VLAAAEYMATGLARQGYQAEYHGTAIVPVILGNPTVAHAGYLRLMRSGVYVNPVAPPAVPEERSGFR TSYLADHRQSDLDRALHVFAGLAEDLTPQGAAL

>Rv0050 ponA1 TB.seq 53661:55694 MW:71119 SEQ ID NO:158

WILLPMVTFTMAYLIVDVPKPGDIRTNQVSTILASDGSEIAKIVPPEGNRVDVNLSQVPMHVRQAVIAA EDRNFYSNPGFSFTGFARAVKNNLFGGDLQGGSTITQQYVKNALVGSAQHGWSGLMRKAKELVIAT KMSGEWSKDDVLQAYLNIIYFGRGAYGISAASKAYFDKPVEQLTVAEGALLAALIRRPSTLDPAVDPE GAHARWNWVLDGMVETKALSPNDRAAQVFPETVPPDLARAENQTKGPNGLIERQVTRELLELFNID EQTLNTQGLWTTTIDPQAQRAAEKAVAKYLDGQDPDMRAAWSIDPHNGAVRAYYGGDNANGFDF AQAGLQTGSSFKVFALVAALEQGIGLGYQVDSSPLTVDGIKITNVEGEGCGTCNIAEALKMSLNTSYY RLMLKLNGGPQAVADAAHQAGIASSFPGVAHTLSEDGKGGPPNNGIVLGQYQTRVIDMASAYATLAA SGIYHPPHFVQKWSANGQVLFDASTADNTGDQRIPKAVADNVTAAMEPIAGYSRGHNLAGGRDSA AKTGTTQFGDTTANKDAWMVGYTPSLSTAVWVGTVKGDEPLVTASGAAIYGSGLPSDIWKATMDGA LKGTSNETFPKPTEVGGYAGVPPPPPPPEVPPSETVIQPTVEIAPGITIPIGPPTTITLAPPPPAPPAAT

PTPPP

>Rv0051 - TB.seq 55694:57373 MW:61210 SEQ ID NO: 159

VTGALSQSSNISPLPLAADLRSADNRDCPSRTDVLGAALANWGGPVGRHALIGRTRLMTPLRVMFAI ALVFLALGWSTKAACLQSTGTGPGDQRVANWDNQRAYYQLCYSDTVPLYGAELLSQGKFPYKSSWI ETDSNGTPQLRYDGQIAVRYMEYPVLTGIYQYLSMAIAKTYTALSKVAPLPWAEWMFFNVAAFGLA LAWLTTVWATSGLAGRRIWDAALVAASPLVIFQIFTNFDALATGLATSGLLAWARRRPVLAGVLIGLG SAAKLYPLLFLYPLLLLGIRAGRLNALARTMAAAAATWLLVNLPVMLLFPRGWSEFFRLNTRRGDDM DSLYNWKSFTGWRGFDPTLGFWEPPLVLNTWTLLFVLCCAAIAYIALTAPHRPRVAQLTFLTVASFL LVNKVWSPQFSLWLVPLAVLALPHRRILLAWMTIDALVWVPRMYΎLYGNPSRSLPEQWFTTTVLLRD IAVMVLCGLWWQIYRPGRDLVRTGGPGALPACGGVDDPVGGVFANAADAPPGRLPSWLRPRLGD EHARERTPDAGRDRTFSGQHRA

>Rv0106 - TB.seq 124372:125565 MW:43701 SEQ ID NO: 160 MRTPVILVAGQDHTDEVTGALLRRTGTVWEHRFDGHWRRMTATLSRGELITTEDALEFAHGCVSC TIRDDLLVLLRRLHRRDNVGRIWHLAPWLEPQPICWAIDHVRVCVGHGYPDGPAALDVRVAAWTC VDCVRWLPQSLGEDELPDGRTVAQVTVGQAEFADLLVLTHPEPVAVAVLRRLAPRARITGGVDRVEL ALAHLDDNSRRGRTDTPHTPLLAGLPPLAADGEVAIVEFSARRPFHPQRLHAAVDLLLDGWRTRGR LWLANRPDQVMWLESAGGGLRVASAGKWLAAMAASEVAYVDLERRLFADLMWVYPFGDRHTAMT VLVCGADPTDIVNALNAALLSDDEMASPQRWQSYVDPFGDWHDDPCHEMPDAAGEFSAHRNSGES

R

>Rv0125 - TB.seq 151146:152210 MW:34927 SEQ ID NO:161

MSNSRRRSLRWSWLLSVLAAVGLGLATAPAQAAPPALSQDRFADFPALPLDPSAMVAQVGPQWNI NTKLGYNNAVGAGTGIVIDPNGWLTNNHVIAGATDINAFSVGSGQTYGVDWGYDRTQDVAVLQLR

GAGGLPSAAIGGGVAVGEPWAMGNSGGQGGTPRAVPGRWALGQTVQASDSLTGAEETLNGLIQ FDAAIQPGDSGGPWNGLGQWGMNTAASDNFQLSQGGQGFAIPIGQAMAIAGQIRSGGGSPTVHI GPTAFLGLGWDNNGNGARVQRWGSAPAASLGISTGDVITAVDGAPINSATAMADALNGHHPGDVI SVTWQTKSGGTRTGNVTLAEGPPA

>Rv0350 dnaK 70 kD heat shock protein, chromosome replication TB.seq 419833:421707 MW:66832 SEQ ID NO:162 MARAVGIDLGTTNSWSVLEGGDPVWANSEGSRTTPSIVAFARNGEVLVGQPAKNQAVTNVDRTV RSVKRHMGSDWSIEIDGKKYTAPEISARILMKLKRDAEAYLGEDITDAVITTPAYFNDAQRQATKDAG QIAGLNVLRIVNEPTAAALAYGLDKGEKEQRILVFDLGGGTFDVSLLEIGEGWEVRATSGDNHLGGD DWDQRWDWLVDKFKGTSGIDLTKDKMAMQRLREAAEKAKIELSSSQSTSINLPYITVDADKNPLFLD EQLTRAEFQRITQDLLDRTRKPFQSVIADTGISVSEIDHWLVGGSTRMPAVTDLVKELTGGKEPNKG VNPDEWAVGAALQAGVLKGEVKDVLLLDVTPLSLGIETKGGVMTRLIERNTTIPTKRSETFTTADDN QPSVQIQVYQGEREIAAHNKLLGSFELTGIPPAPRGIPQIEVTFDIDANGIVHVTAKDKGTGKENTIRIQ EGSGLSKEDIDRMIKDAEAHAEEDRKRREEADVRNQAETLVYQTEKFVKEQREAEGGSKVPEDTLN KVDAAVAEAKAALGGSDISAIKSAMEKLGQESQALGQAIYEAAQAASQATGAAHPGGEPGGAHPGS ADDWDAEWDDGREAK

>Rv0351 grpE stimulates DnaK ATPase activity TB.seq 421707:422411 MW:24501 SEQ ID NO:163

VTDGNQKPDGNSGEQVTVTDKRRIDPETGEVRHVPPGDMPGGTAAADAAHTEDKVAELTADLQRV QADFANYRKRALRDQQAAADRAKASWSQLLGVLDDLERARKHGDLESGPLKSVADKLDSALTGLG LVAFGAEGEDFDPVLHEAVQHEGDGGQGSKPVIGTVMRQGYQLGEQVLRHALVGWDTVWDAAE LESVDDGTAVADTAENDQADQGNSADTSGEQAESEPSGS

>Rv0352 dnaJ acts with GrpE to stimulate DnaK ATPase TB.seq 422450:423634 MW:41346 SEQ ID NO:164

MAQREWVEKDFYQELGVSSDASPEEIKRAYRKLARDLHPDANPGNPAAGERFKAVSEAHNVLSDPA KRKEYDETRRLFAGGGFGGRRFDSGFGGGFGGFGVGGDGAEFNLNDLFDAASRTGGTTIGDLFGG LFGRGGSARPSRPRRGNDLETETELDFVEAAKGVAMPLRLTSPAPCTNCHGSGARPGTSPKVCPTC NGSGVINRNQGAFGFSEPCTDCRGSGSIIEHPCEECKGTGVTTRTRTINVRIPPGVEDGQRIRLAGQ GEAGLRGAPSGDLYVTVHVRPDKIFGRDGDDLTVTVPVSFTELALGSTLSVPTLDGTVGVRVPKGTA DGRILRVRGRGVPKRSGGSGDLLVTVKVAVPPNLAGAAQEALEAYAAAERSSGFNPRAGWAGNR

>Rv0363c fba fructose bisphosphate aldolase TB.seq 441266:442297 MW:36545 SEQ ID NO:165

MPIATPEVYAEMLGQAKQNSYAFPAINCTSSETVNAAIKGFADAGSDGIIQFSTGGAEFGSGLGVKDM VTGAVALAEFTHVIAAKYPVNVALHTDHCPKDKLDSYVRPLLAISAQRVSKGGNPLFQSHMWDGSAV PIDENLAIAQELLKAAAAAKIILEIEIGWGGEEDGVANEINEKLYTSPEDFEKTIEALGAGEHGKYLLAA TFGNVHGVYKPGNVKLRPDILAQGQQVAAAKLGLPADAKPFDFVFHGGSGSLKSEIEEALRYGWKM NVDTDTQYAFTRPIAGHMFTNYDGVLKVDGEVGVKKVYDPRSYLKKAEASMSQRWQACNDLHCA

GKSLTH >Rv0405 pks6 TB.seq 485729:489934 MW:147615 SEQ ID NO:166

MTDGSVTADKLQKWFREYLSTHIECHPNEVSLDVPIRDLGLKSIDVLAIPGDLGDRFGFCIPDLAVWD NPSANDLIDSLLNQRSADSLRESHGHADRNTQGRGSINEPVAVIGVGCRFPGDIDGPERLWDFLTEK KCAITAYPDRGFTNAGTFAESGGFLKDVAGFDNRFFDIPPDEALRMDPQQRLLLEVSWEALEHAGIIP ESLRLSRTGVFVGVSSTDYVRLVSASAQQKSTIWDNTGGSSSIIANRISYFLDIQGPSIVIDTACSSSLV AVHLACRSLSTWDCDIALVGGTNVLISPEPWGGFREAGILSQTGCCHAFDKSADGMVRGEGCGVIVL QRLSDARLEGRRILAILTGSAVN^QDGKSNGIMAPNPSAQIGVLENACKSARVDPLEIGYVEAHGTGTS LGDRIEAHALGMVFGRKRPGSGPLMIGSIKPNIGHLEGAAGIAGLIKAVLMVERGSLLPSGGFTEPNP AIPFTELGLRWDELQEWPWAGRPRRAGVSSFGFGGTNAHVIVEEAGSVGADTVSGRADVGGSGG GWAWVISGKTASALAAQAGRLGRYVRARPALDWDVGYSLVSTRSVFDHRAWVGQTRDELLAGL AGWAGRPEAGWCGVGKPAGKTAFVFAGQGSQWLGMGSELYAAYPVFAEALDAWDELDRHLRY PLRDVIWGHDQDLLNTTEFAQPALFAVEVALYRLLMSWGVRPGLVLGHSVGELAAAHVAGALCLPD AAMLVAARGRLMQALPAGGAMFAVQAREDEVAPMLGHDVSIAAVNGPASWISGAHDAVSAIADRL RGQGRRVHRLAVSHAFHSALMEPMIAEFTAVAAELSVGLPTIPVISNVTGQLVADDFASADYWARHIR AWRFGDSVRSAHCAGASRFIEVGPGGGLTSLIEASLADAQIVSVPTLRKDRPEPVSVMTAAAQGFV SGMGLDWASVFSGYRPKRVELPTYAFQHQKFWLAPAPSVSDPTAAGQIGASDGGAELLASSGFAA RLAGRSADEQLAAAIEWCEHAAAVLGRDGAAGLDAGQAFADSGFNSLSAVELRNRLTAVTAVTLPA TAIFDHPTPTELAQYLITQIDGHGSSAAAAANPAERIDALTDLFLQACDAGRDADGWKMVALASNTRE RMSSPVRNNVSKNVALLADGISDWVICIPTLTVLSDQREYRDIANAMTGRHSVYSLTLPGFDSSDAL PQNADMIVETVSNAIIDWGGSCRFVLSGYSSGGVLAYALCSHLSVKHQRNPLGVALIDTYLPSQIAN PSMNEGFSPNDTGKGLSREVIRVARMLNRLTATRLTAAATYAAIFQAWEPGRSMAPVLNIVAKDRIAT VENLREERINRWRTAAAEAAYSVAEVPGDHFGMMSTSSEAIATEIHDWISGLVRGPHR

>Rv0435c - ATPase of AAA-family TB.seq 522348:524531 MW:75315 SEQ ID NO: 167

VTH PDPARQLTLTARLNTSAVDSRRGNΛ RLH PNAI AALGI REWDAVSLTGSRTTAAVAGLAAADTAV

GTVLLDDVTLSNAGLREGTEVIVSPVTVYGARSVTLSGSTLATQSVPPVTLRQALLGKVMTVGDAVSL

LPRDLGPGTSTSAASRALAAAVGISWTSELLTVTGVDPDGPVSVQPNSLVTWGAGVPAAMGTSTAG QVSISSPEIQIEELKGAQPQAAKLTEWLKLALDEPHLLQTLGAGTNLGVLVSGPAGVGKATLVRAVCD GRRLVTLDGPEIGALAAGDRVKAVASAVQAVRHEGGVLLITDADALLPAAAEPVASLILSELRTAVATA GWLIATSARPDQLDARLRSPELCDRELGLPLPDAATRKSLLEALLNPVPTGDLNLDEIASRTPGFWA DLAALVREAALRAASRASADGRPPMLHQDDLLGALTVIRPLSRSASDEVTVGDVTLDDVGDMAAAK QALTEAVLWPLQHPDTFARLGVEPPRGVLLYGPPGCGKTFWRALASTGQLSVHAVKGSELMDKWV GSSEKAVRELFRRARDSAPSLVFLDELDALAPRRGQSFDSGVSDRWAALLTELDGIDPLRDWMLG ATNRPDLIDPALLRPGRLERLVFVEPPDAAARREILRTAGKSIPLSSDVDLDEVAAGLDGYSAADCVAL LREAALTAMRRSIDAANVTAADLATARETVRASLDPLQVASLRKFGTKGDLRS

>Rv0436c pssA CDP-diacylglycerol-serine o-phosphatidyltransferase TB.seq 524531 :525388 MW:31219 SEQ ID NO:168

MIGKPRGRRGVNLQILPSAMTVLSICAGLTAIKFALEHQPKAAMALIAAAAILDGLDGRVARILDAQSR MGAEIDSLADAVNFGVTPALVLYVSMLSKWPVGWWVLLYAVCWLRLARYNALQDDGTQPAYAHE FFVGMPAPAGAVSMIGLLALKMQFGEGWWTSGWFLSFWVTGTSILLVSGIPMKKMHAVSVPPNYAA

ALLAVLAICAAAAVLAPYLLIWVIIIAYMCHIPFAVRSQRWLAQHPEVWDDKPKQRRAVRRASRRAHP

YRPSMARLGLRKPGRRL

>Rv0440 groEL 260 kD chaperonin 2 TB.seq 528606:530225 MW:56728 SEQ ID NO: 169

MAKTIAYDEEARRGLERGLNALADAVKVTLGPKGRNWLEKKWGAPTITNDGVSIAKEIELEDPYEKI GAELVKEVAKKTDDVAGDGTTTATVLAQALVREGLRNVAAGANPLGLKRGIEKAVEKVTETLLKGAK EVETKEQIAATAAISAGDQSIGDLIAEAMDKVGNEGVITVEESNTFGLQLELTEGMRFDKGYISGYFVT DPERQEAVLEDPYILLVSSKVSTVKDLLPLLEKVIGAGKPLLIIAEDVEGEALSTLWNKIRGTFKSVAVK APGFGDRRKAMLQDMAILTGGQVISEEVGLTLENADLSLLGKARKVWTKDETTIVEGAGDTDAIAGR VAQIRQEIENSDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKHRIEDAVRNAKAAVEEGIVA GGGVTLLQAAPTLDELKLEGDEATGANIVKVALEAPLKQIAFNSGLEPGWAEKVRNLPAGHGLNAQT GVYEDLLAAGVADPVKVTRSALQNAASIAGLFLTTEAWADKPEKEKASVPGGGDMGGMDF

>Rv0482 murB TB.seq 570537:571643 MW:38522 SEQ ID NO:170

MKRSGVGSLFAGAHIAEAVPLAPLTTLRVGPIARRVITCTSAEQWAALRHLDSAAKTGADRPLVFAG GSNLVIAENLTDLTWRLANSGITIDGNLVRAEAGAVFDDVWRAIEQGLGGLECLSGIPGSAGATPVQ NVGAYGAEVSDTITRVRLLDRCTGEVRWVSARDLRFGYRTSVLKHADGLAVPTWLEVEFALDPSGR SAPLRYGELIAALNATSGERADPQAVREAVLALRARKGMVLDPTDHDTWSVGSFFTNPWTQDVYE RLAGDAATRKDGPVPHYPAPDGVKLAAGWLVERAGFGKGYPDAGAAPCRLSTKHALALTNRGGAT AEDWTLARAVRDGVHDVFGITLKPEPVLIGCML

>Rv0483 - TB.seq 571708:573060 MW:47859 SEQ ID NO:171

WIRVLFRPVSLIPVNNSSTPQSQGPISRRLALTALGFGVLAPNVLVACAGKVTKLAEKRPPPAPRLTF RPADSAADWPIAPISVEVGDGWFQRVALTNSAGKWAGAYSRDRTIYTITEPLGYDTTYTWSGSAV GHDGKAVPVAGKFTTVAPVKTINAGFQLADGQTVGIAAPVIIQFDSPISDKAAVERALTVTTDPPVEGG WAWLPDEAQGARVHWRPREYYPAGTTVDVDAKLYGLPFGDGAYGAQDMSLHFQIGRRQWKAEV SSHRIQWTDAGVIMDFPCSYGEADLARNVTRNGIHWTEKYSDFYMSNPAAGYSHIHERWAVRISN NGEFIHANPMSAGAQGNSNVTNGCINLSTENAEQYYRSAVYGDPVEVTGSSIQLSYADGDIWDWAV DWDTWVSMSALPPPAAKPAATQIPVTAPVTPSDAPTPSGTPTTTNGPGG

>Rv0489 gpm phosphoglycerate mutase I TB.seq 578424:579170 MW:27217 SEQ ID NO: 172

MANTGSLVLLRHGESDWNALNLFTGWVDVGLTDKGQAEAVRSGELIAEHDLLPDVLYTSLLRRAITT AHLALDSADRLWIPVRRSWRLNERHYGALQGLDKAETKARYGEEQFMAWRRSYDTPPPPIERGSQ FSQDADPRYADIGGGPLTECLADWARFLPYFTDVIVGDLRVGKTVLIVAHGNSLRALVKHLDQMSDD EIVGLNIPTGIPLRYDLDSAMRPLVRGGTYLDPEAAAAGAAAVAGQGRG >Rv0490 senX 3sensor histidine kinase TB.seq 579347:580576 MW:44794 SEQ ID NO:173

VTVFSALLLAGVLSALALAVGGAVGMRLTSRWEQRQRVATEWSGITVSQMLQCIVTLMPLGAAWD THRDWYLNERAKELGLVRDRQLDDQAWRAARQALGGEDVEFDLSPRKRSATGRSGLSVHGHARL LSEEDRRFAWFVHDQSDYARMEAARRDFVANVSHELKTPVGAMALLAEALLASADDSETVRRFAE KVLIEANRLGDMVAELIELSRLQGAERLPNMTDVDVDTIVSEAISRHKVAADNADIEVRTDAPSNLRVL GDQTLLVTALANLVSNAIAYSPRGSLVSISRRRRGANIEIAVTDRGIGIAPEDQERVFERFFRGDKARS RATGGSGLGLAIVKHVAANHDGTIRVWSKPGTGSTFTLALPALIEAYHDDERPEQAREPELRSNRSQ REEELSR

>Rv0500 proC pyrroline-5-carboxylate reductase TB.seq 590081:590965 MW:30172

SEQ ID NO:174

MLFGMARIAIIGGGSIGEALLSGLLRAGRQVKDLWAERMPDRANYLAQTYSVLVTSAADAVENATFV WAVKPADVEPVIADLANATAAAENDSAEQVFVTWAGITIAYFESKLPAGTPWRAMPNAAALVGAG VTALAKGRFVTPQQLEEVSALFDAVGGVLTVPESQLDAVTAVSGSGPAYFFLLVEALVDAGVGVGLS RQVATDLAAQTMAGSAAMLLERMEQDQGGANGELMGLRVDLTASRLRAAVTSPGGTTAAALRELE RGGFRMAVDAAVQAAKSRSEQLRITPE

>Rv0528 - TB.seq 618303:619889 MW:57132 SEQ ID NO:175

MWRSLTSMGTALVLLFLLALAAIPGALLPQRGLNAAKVDDYLAAHPLIGPWLDELQAFDVFSSFWFTA IYVLLFVSLVGCLAPRTIEHARSLRATPVAAPRNLARLPKHAHARLAGEPAALAATITGRLRGWRSITR QQGDSVEVSAEKGYLREFGNLVFHFALLGLLVAVAVGKLFGYEGNVIVIADGGPGFCSASPAAFDSF RAGNTVDGTSLHPICVRVNNFQAHYLPSGQATSFAADIDYQADPATADLIANSWRPYRLQVNHPLRV GGDRVYLQGHGYAPTFTVTFPDGQTRTSTVQWRPDNPQTLLSAGWRIDPPAGSYPNPDERRKHQI AIQGLLAPTEQLDGTLLSSRFPALNAPAVAIDIYRGDTGLDSGRPQSLFTLDHRLIEQGRLVKEKRVNL RAGQQVRIDQGPAAGTWRFDGAVPFVNLQVSHDPGQSWVLVFAITMMAGLLVSLLVRRRRVWARI TPTTAGTVNVELGGLTRTDNSGWGAEFERLTGRLLAGFEARSPDMAEAAAGTGRDVD

>Rv0667 rpoB [beta] subunit of RNA polymerase TB.seq 759805:763320 MW: 129220 SEQ ID NO:176 LADSRQSKTAASPSPSRPQSSSNNSVPGAPNRVSFAKLREPLEVPGLLDVQTDSFEWLIGSPRWRE SAAERGDVNPVGGLEEVLYELSPIEDFSGSMSLSFSDPRFDDVKAPVDECKDKDMTYAAPLFVTAEF INNNTGEIKSQTVFMGDFPMMTEKGTFIINGTERVWSQLVRSPGVYFDETIDKSTDKTLHSVKVIPSR GAWLEFDVDKRDTVGVRIDRKRRQPVTVLLKALGWTSEQIVERFGFSEIMRSTLEKDNTVGTDEALL DIYRKLRPGEPPTKESAQTLLENLFFKEKRYDLARVGRYKVNKKLGLHVGEPITSSTLTEEDWATIEY LVRLHEGQTTMTVPGGVEVPVETDDIDHFGNRRLRTVGELIQNQIRVGMSRMERWRERMTTQDVE AITPQTLINIRPWAAIKEFFGTSQLSQFMDQNNPLSGLTHKRRLSALGPGGLSRERAGLEVRDVHPS HYGRMCPIETPEGPNIGLIGSLSVYARVNPFGFIETPYRKWDGWSDEIVYLTADEEDRHWAQANS PIDADGRFVEPRVLVRRKAGEVEYVPSSEVDYMDVSPRQMVSVATAMIPFLEHDDANRALMGANMQ RQAVPLVRSEAPLVGTGMELRAAIDAGDVWAEESGVIEEVSADYITVMHDNGTRRTYRMRKFARSN HGTCANQCPIVDAGDRVEAGQVIADGPCTDDGEMALGKNLLVAIMPWEGHNYEDAIILSNRLVEEDV LTSIHIEEHEIDARDTKLGAEEITRDIPNISDEVLADLDERGIVRIGAEVRDGDILVGKVTPKGETELTPE ERLLRAIFGEKAREVRDTSLKVPHGESGKVIGIRVFSREDEDELPAGVNELVRVYVAQKRKISDGDKL AGRHGNKGVIGKILPVEDMPFLADGTPVDIILNTHGVPRRMNIGQILETHLGWCAHSGWKVDAAKGV PDWAARLPDELLEAQPNAIVSTPVFDGAQEAELQGLLSCTLPNRDGDVLVDADGKAMLFDGRSGEP FPYPVTVGYMYIMKLHHLVDDKIHARSTGPYSMITQQPLGGKAQFGGQRFGEMECWAMQAYGAAY TLQELLTIKSDDTVGRVKVYEAIVKGENIPEPGIPESFKVLLKELQSLCLNVEVLSSDGAAIELREGEDE DLERAAANLGINLSRNESASVEDLA

>Rv0668 rpoC [beta]' subunit of RNA polymerase TB.seq 763368:767315 MW:146740 SEQ ID NO:177

VLDVNFFDELRIGLATAEDIRQWSYGEVKKPETINYRTLKPEKDGLFCEKIFGPTRDWECYCGKYKRV RFKGIICERCGVEVTRAKVRRERMGHIELAAPVTHIWYFKGVPSRLGYLLDLAPKDLEKIIYFAAYVITS VDEEMRHNELSTLEAEMAVERKAVEDQRDGELEARAQKLEADLAELEAEGAKADARRKVRDGGER EMRQIRDRAQRELDRLEDIWSTFTKLAPKQLIVDENLYRELVDRYGEYFTGAMGAESIQKLIENFDIDA EAESLRDVIRNGKGQKKLRALKRLKWAAFQQSGNSPMGMVLDAVPVIPPELRPMVQLDGGRFATS DLNDLYRRVINRNNRLKRLIDLGAPEIIVNNEKRMLQESVDALFDNGRRGRPVTGPGNRPLKSLSDLL KGKQGRFRQNLLGKRVDYSGRSVIWGPQLKLHQCGLPKLMALELFKPFVMKRLVDLNHAQNIKSAK RMVERQRPQVWDVLEEVIAEHPVLLNRAPTLHRLGIQAFEPMLVEGKAIQLHPLVCEAFNADFDGDQ MAVHLPLSAEAQAEARILMLSSNNILSPASGRPLAMPRLDMVTGLYYLTTEVPGDTGEYQPASGDHP ETGVYSSPAEAIMAADRGVLSVRAKIKVRLTQLRPPVEIEAELFGHSGWQPGDAWMAETTLGRVMF NELLPLGYPFVNKQMHKKVQAAIINDLAERYPMIWAQTVDKLKDAGFYWATRSGVTVSMADVLVPP RKKEILDHYEERADKVEKQFQRGALNHDERNEALVEIWKEATDEVGQALREHYPDDNPIITIVDSGAT GNFTQTRTLAGMKGLVTNPKGEFIPRPVKSSFREGLTVLEYFINTHGARKGLADTALRTADSGYLTRR LVDVSQDVIVREHDCQTERGIWELAERAPDGTLIRDPYIETSAYARTLGTDAVDEAGNVIVERGQDL GDPEIDALLAAGITQVKVRSVLTCATSTGVCATCYGRSMATGKLVDIGEAVGIVAAQSIGEPGTQLTM RTFHQGGVGEDITGGLPRVQELFEARVPRGKAPIADVTGRVRLEDGERFYKITIVPDDGGEEWYDKI SKRQRLRVFKHEDGSERVLSDGDHVEVGQQLMEGSADPHEVLRVQGPREVQIHLVREVQEVYRAQ GVSIHDKHIEVIVRQMLRRVTIIDSGSTEFLPGSLIDRAEFEAENRRWAEGGEPAAGRPVLMGITKAS LATDSWLSAASFQETTRVLTDAAINCRSDKLNGLKENVIIGKLIPAGTGINRYRNIAVQPTEEARAAAYT IPSYEDQYYSPDFGAATGAAVPLDDYGYSDYR

>Rv0711 atsA TB.seq 806333:808693 MW:86216 SEQ ID NO:178

MAPEATEAFNGTIELDIRDSEPDWGPYAAPVAPEHSPNILYLVWDDVGIATWDCFGGLVEMPAMTRV AERGVRLSQFHTTALCSPTRASLLTGRNATTVGMATIEEFTDGFPNCNGRIPADTALLPEVLAEHGYN TYCVGKWHLTPLEESNMASTKRHWPTSRGFERFYGFLGGETDQWYPDLVYDNHPVSPPGTPEGG YHLSKDIADKTIEFIRDAKVIAPDKPWFSYVCPGAGHAPHHVFKEWADRYAGRFDMGYERYREIVLE RQKALGIVPPDTELSPINPYLDVPGPNGETWPLQDTVRPWDSLSDEEKKLFCRMAEVFAGFLSYTDA QIGRILDYLEESGQLDNTIIWISDNGASGEGGPNGSVNEGKFFNGYIDTVAESMKLFDHLGGPQTYN HYPIGWAMAFNTPYKLFKRYASHEGGIADPAIISWPNGIAAHGEIRDNYVNVSDITPTVYDLLGMTPP GTVKGIPQKPMDGVSFIAALADPAADTGKTTQFYTMLGTRGIWHEGWFANTIHAATPAGWSNFNAD RWELFHIAADRSQCHDLAAEHPDKLEELKALWFSEAAKYNGLPLADLNLLETMTRSRPYLVSERASY VYYPDCADVGIGAAVEIRGRSFAVLADVTIDTTGAEGVLFKHGGAHGGHVLFVRDGRLHYVYNFLGE RQQLVSSSGPVPSGRHLLGVRYLRTGTVPNSHTPVGDLELFFDENLVGALTNVLTHPGTFGLAGAAI SVGRNGGSAVSSHYEAPFAFTGGTITQVTVDVSGRPFEDVESDLALAFSRD

>Rv0764c - lanosterol 14-demethylase cytochrome P450 TB.seq 856683:858035 MW:50879

SEQ ID NO:179

MSAVALPRVSGGHDEHGHLEEFRTDPIGLMQRVRDECGDVGTFQLAGKQWLLSGSHANEFFFRA GDDDLDQAKAYPFMTPIFGEGWFDASPERRKEMLHNAALRGEQMKGHAATIEDQVRRMIADWGE

AGEIDLLDFFAELTIYTSSACLIGKKFRDQLDGRFAKLYHELERGTDPLAYVDPYLPIESFRRRDEARN GLVALVADIMNGRIANPPTDKSDRDMLDVLIAVKAETGTPRFSADEITGMFISMMFAGHHTSSGTASW TLIELMRHRDAYAAVIDELDELYGDGRSVSFHALRQIPQLENVLKETLRLHPPLIILMRVAKGEFEVQG HRIHEGDLVAASPAISNRIPEDFPDPHDFVPARYEQPRQEDLLNRWTWIPFGAGRHRCVGAAFAIMQI KAIFSVLLREYEFEMAQPPESYRNDHSKMWQLAQPACVRYRRRTGV

>Rv0861c - DNA helicase TB.seq 958524:960149 MW:59773 SEQ ID NO:180

VQSDKTVLLEVDHELAGAARAAIAPFAELERAPEHVHTYRITPLALWNARAAGHDAEQWDALVSYS RYAVPQPLLVDIVDTMARYGRLQLVKNPAHGLTLVSLDRAVLEEVLRNKKIAPMLGARIDDDTVWHP SERGRVKQLLLKIGWPAEDLAGYVDGEAHPISLHQEGWQLRDYQRLAADSFWAGGSGWVLPCGA GKTLVGAAAMAKAGATTLILVTNIVAARQWKRELVARTSLTENEIGEFSGERKEIRPVTISTYQMITRR TKGEYRHLELFDSRDWGLIIYDEVHLLPAPVFRMTADLQSKRRLGLTATLIREDGREGDVFSLIGPKR YDAPWKDIEAQGWIAPAECVEVRVTMTDSERMMYATAEPEERYRICSTVHTKIAWKSILAKHPDEQ TLVIGAYLDQLDELGAELGAPVIQGSTRTSEREALFDAFRRGEVATLWSKVANFSIDLPEAAVAVQVS GTFGSRQEEAQRLGRILRPKADGGGAIFYSWARDSLDAEYAAHRQRFLAEQGYGYIIRDADDLLGP

Al

>Rv0904c accD3 TB.seq 1006694:1008178 MW:51741 SEQ ID NO:181

VSRITTDQLRHAVLDRGSFVSWDSEPLAVPVADSYARELAAARAATGADESVQTGEGRVFGRRVAV VACEFDFLGGSIGVAAAERITAAVERATAERLPLLASPSSGGTRMQEGTVAFLQMVKIAAAIQLHNQA RLPYLVYLRHPTTGGVFASWGSLGHLTVAEPGALIGFLGPRVYELLYGDPFPSGVQTAENLRRHGIID GWALDRLRPMLDRALTVLIDAPEPLPAPQTPAPVPDVPTWDSWASRRPDRPGVRQLLRHGATDR VLLSGTDQGEAATTLLALARFGGQPTWLGQQRAVGGGGSTVGPAALREARRGMALAAELCLPLVL VIDAAGPALSAAAEQGGLAGQIAHCLAELVTLDTPTVSILLGQGSGGPALAMLPADRVLAALHGWLAP LPPEGASAIVFRDTAHAAELAAAQGIRSADLLKSGIVDTIVPEYPDAADEPIEFALRLSNAIAAEVHALR KIPAPERLATRLQRYRRIGLPRD

>Rv0983 - TB.seq 1099064:1100455 MW.46454 SEQ ID NO: 182

MAKLARWGLVQEEQPSDMTNHPRYSPPPQQPGTPGYAQGQQQTYSQQFDWRYPPSPPPQPTQY RQPYEALGGTRPGLIPGVIPTMTPPPGMVRQRPRAGMLAIGAVTIAWSAGIGGAAASLVGFNRAPA GPSGGPVAASAAPSIPAANMPPGSVEQVAAKWPSWMLETDLGRQSEEGSGIILSAEGLILTNNHVI AAAAKPPLGSPPPKTTVTFSDGRTAPFTWGADPTSDIAWRVQGVSGLTPISLGSSSDLRVGQPVLA IGSPLGLEGTVTTGIVSALNRPVSTTGEAGNQNTVLDAIQTDAAINPGNSGGALVNMNAQLVGVNSAI ATLGADSADAQSGSIGLGFAIPVDQAKRIADELISTGKASHASLGVQVTNDKDTLGAKIVEWAGGAA ANAGVPKGNΛ VTKVDDRPINSADALVAAVRSKAPGATVALTFQDPSGGSRTVQVTLGKAEQ

>Rv1008 - Similar to E.coli protein YcfH TB.seq 1127087: 1127878 MW:29066 SEQ ID NO:183

LVDAHTHLDACGARDADTVRSLVERAAAAGVTAWTVADDLESARWVTRAAEWDRRVYAAVALHPT RADALTDAARAELERLVAHPRWAVGETGIDMYWPGRLDGCAEPHVQREAFAWHIDLAKRTGKPLM IHNRQADRDVLDVLRAEGAPDTVILHCFSSDAAMARTCVDAGWLLSLSGTVSFRTARELREAVPLMP VEQLLVETDAPYLTPHPHRGLANEPYCLPYTVRALAELVNRRPEEVALITTSNARRAYGLGWMRQ

>Rv1009 - lipoprotein, similar to various other MTB proteins TB.seq 1128089:1129174 MW:38079 SEQ ID NO:184

MLRLWGALLLVLAFAGGYAVAACKTVTLTVDGTAMRVTTMKSRVIDIVEENGFSVDDRDDLYPAAG VQVHDADTIVLRRSRPLQISLDGHDAKQVWTTASTVDEALAQLAMTDTAPAAASRASRVPLSGMALP WSAKTVQLNDGGLVRTVHLPAPNVAGLLSAAGVPLLQSDHWPAATAPIVEGMQIQVTRNRIKKVTE RLPLPPNARRVEDPEMNMSREWEDPGVPGTQDVTFAVAEVNGVETGRLPVANVWTPAHEAWR VGTKPGTEVPPVIDGSIWDAIAGCEAGGNWAINTGNGYYGGVQFDQGTWEANGGLRYAPRADLAT REEQIAVAEVTRLRQGWGAWPVCAARAGAR

>Rv1010 ksgA 16S rRNA dimethyltransferase TB.seq 1129150:1130100 MW:34647

SEQ ID NO:185

MCCTSGCALTIRLLGRTEIRRLAKELDFRPRKSLGQNFVHDANTVRRWAASGVSRSDLVLEVGPGL GSLTLALLDRGATVTAVEIDPLLASRLQQ AEHSHSEVHRLTWNRDVLALRREDLAAAPTAWANL PYNVAVPALLHLLVEFPSIRWTVMVQAEVAERLAAEPGSKEYGVPSVKLRFFGRVRRCGMVSPTVF WPIPRVYSGLVRIDRYETSPWPTDDAFRRRVFELVDIAFAQRRKTSRNAFVQWAGSGSESANRLLAA SIDPARRGETLSIDDFVRLLRRSGGSDEATSTGRDARAPDISGHASAS >Rv1011 - Similar to E.coli protein YcbH TB.seq 1130189:1131106 MW:31350

SEQ ID NO:186

VPTGSVTVRVPGKVNLYLAVGDRREDGYHELTTVFHAVSLVDEVTVRNADVLSLELVGEGADQLPTD ERNLAWQAAELMAEHVGRAPDVSIMIDKSIPVAGGMAGGSADAAAVLVAMNSLWELNVPRRDLRML AARLGSDVPFALHGGTALGTGRGEELATVLSRNTFHWVLAFADSGLLTSAVYNELDRLREVGDPPRL GEPGPVLAALAAGDPDQLAPLLGNEMQAAAVSLDPALARALRAGVEAGALAGIVSGSGPTCAFLCTS ASSAIDVGAQLSGAGVCRTVRVATGPVPGARWSAPTEV

>Rv1106c - cholesterol dehydrogenase TB.seq 1232845:1233954 MW:40743 SEQ ID NO:187 MLRRMGDASLTTELGRVLVTGGAGFVGANLVTTLLDRGHWVRSFDRAPSLLPAHPQLEVLQGDITD ADVCAAAVDGIDTIFHTAAIIELMGGASVTDEYRQRSFAVNVGGTENLLHAGQRAGVQRFVYTSSNS WMGGQNIAGGDETLPYTDRFNDLYTETKWAERFVLAQNGVDGMLTCAIRPSGIWGNGDQTMFRK LFESVLKGHVKVLVGRKSARLDNSYVHNLIHGFILAAAHLVPDGTAPGQAYFINDAEPINMFEFARPVL EACGQRWPKMRISGPAVRWVMTGWQRLHFRFGFPAPLLEPLAVERLYLDNYFSIAKARRDLGYEPL FTTQQALTECLPYYVSLFEQMKNEARAEKTAATVKP

>Rv1110 lytB2 TB.seq 1236183:1237187 MW:36298 SEQ ID NO:188

MVPTVDMGIPGASVSSRSVADRPNRKRVLLAEPRGYCAGVDRAVETVERALQKHGPPVYVRHEIVH NRHWDTLAKAGAVFVEETEQVPEGAINΛ/FSAHGVAPTVHVSASERNLQVIDATCPLVTKVHNEARR FARDDYDILLIGHEGHEEWGTAGEAPDHVQLVDGVDAVDQVTVRDEDKWWLSQTTLSVDETMEIV GRLRRRFPKLQDPPSDDICYATQNRQVAVKAMAPECELVIWGSRNSSNSVRLVEVALGAGARAAH LVDWADDIDSAWLDGVTTVGVTSGASVPEVLVRGVLERLAECGYDIVQPVTTANETLVFALPRELRS

PR

>Rv1216c - TB.seq 1359473:1360144 MW:24863 SEQ ID NO:189

MHIGLKIFIWGVLGLWFGALLFGPAGTFDYWQAWVFLAAFVSTTIGPTIYLARNDPAALQRRMRSGP LAEGRTIQKFIVIGAFLGFFAMMVLSACDHRYGWSSVPAAVCVIGDVLVMTGLGIAMLWIQNRYAAS TVRVEAGQILASDGLYKIVRHPMYAGNWMMTGIPLALGSYWAMFILVPGTLVLVFRILDEEKLLTQEL SGYREYRQLVRYRLVPYVW

>Rv1223 htrA TB.seq 1365810:1367456 MW:56547 SEQ ID NO:190

VSHLSQRMAGLLRVHGEWSRSVDTRVDTDNAMPARFSAQIQNEDEVTSDQGNNGGPNGGGRLAP RPVFRPPVDPASRQAFGRPSGVQGSFVAERVRPQKYQDQSDFTPNDQLADPVLQEAFGRPFAGAE SLQRHPIDAGALAAEKDGAGPDEPDDPWRDPAAAAALGTPALAAPAPHGALAGSGKLGVRDVLFGG KVSYLALGILVAIALVIGGIGGVIGRKTAEWDAFTTSKVTLSTTGNAQEPAGRFTKVAAAVADSWTIE SVSDQEGMQGSGVIVDGRGYIVTNNHVISEAANNPSQFKTTWFNDGKEVPANLVGRDPKTDLAVLK VDNVDNLTVARLGDSSKVRVGDEVLAVGAPLGLRSTVTQGIVSALHRPVPLSGEGSDTDTVIDAIQTD ASINHGNSGGPLIDMDAQVIGINTAGKSLSDSASGLGFAIPVNEMKLVANSLIKDGKIVHPTLGISTRSV

SNAIASGAQVANVKAGSPAQKGGILENDVIVKVGNRAVADSDEFWAVRQLAIGQDAPIEWREGRH

VTLTVKPDPDST

>Rv1224 - TB.seq 1367461:1367853 MW:14083 SEQ ID NO:191

VFANIGWWEMLVLVMVGLWLGPERLPGAIRWAASALRQARDYLSGVTSQLREDIGPEFDDLRGHL GELQKLRGMTPRAALTKHLLDGDDSLFTGDFDRPTPKKPDAAGSAGPDATEQIGAGPIPFDSDAT

>Rv1229c mrp similar to MRP/NBP35 ATP-binding proteins TB.seq 1371778:1372947 MW:41064 SEQ ID NO:192

MPSRLHSAVMSGTRDGDLNAAIRTALGKVIDPELRRPITELGMVKSIDTGPDGSVHVEIYLTIAGCPKK SEITERVTRAVADVPGTSAVRVSLDVMSDEQRTELRKQLRGDTREPVIPFAQPDSLTRVYAVASGKG GVGKSTVTVNLAAAMAVRGLSIGVLDADIHGHSIPRMMGTTDRPTQVESMILPPIAHQVKVISIAQFTQ GNTPWWRGPMLHRALQQFLADVYWGDLDVLLLDLPPGTGDVAISVAQLIPNAELLWTTPQLAAAE VAERAGSIALQTRQRIVGWENMSGLTLPDGTTMQVFGEGGGRLVAERLSRAVGADVPLLGQIPLDP ALVAAGDSGVPLVLSSPDSAIGKELHSIADGLSTRRRGLAGMSLGLDPTRR

>Rv1239c corA magnesium and cobalt transport protein TB.seq 1381943:1383040 MW.41470 SEQ ID NO:193 VFPGFDALPEVLRPVARPQPPNAHPVAQPPAQALVDCGVYVCGQRLPGKYTYAAALREVREIELTG QEAFVWIGLHEPDENQMQDVADVFGLHPLAVEDAVHAHQRPKLERYDETLFLVLKTVNYVPHESW LAREIVKTGEIMIFVGKDFWTVRHGEHGGLSEVRKRMDADPEHLRLGPYAVMHAIADYWDHYLEVT NLMETDIDSIEEVAFAPGRKLDIEPIYLLKREWELRRCVNPLSTAFQRMQTESKDLISKEVRRYLRDV ADHQTEAADQIASYDDMLNSLVQAALARVGMQQNMDMRKISAWAGIIAVPTMIAGIYGMNFHFMPEL DSRWGYPTVIGGMVLICLFLYHVFRNRNWL

>RV1279 - TB.seq 1430060:1431643 MW:57332 SEQ ID NO:194

MDTQSDYVWGTGSAGAWASRLSTDPATTWALEAGPRDKNRFIGVPAAFSKLFRSEIDWDYLTEP QPELDGREIYWPRGKVLGGSSSMNAMMWVRGFASDYDEWAARAGPRWSYADVLGYFRRIENVTA AWHFVSGDDSGVTGPLHISRQRSPRSVTAAWLAAARECGFAAARPNSPRPEGFCETWTQRRGAR FSTADAYLKPAMRRKNLRVLTGATATRWIDGDRAVGVEYQSDGQTRIVYARREWLCAGAVNSPQL LMLSGIGDRDHLAEHDIDTVYHAPEVGCNLLDHLVTVLGFDVEKDSLFAAEKPGQLISYLLRRRGMLT SNVGEAYGFVRSRPELKLPDLELIFAPAPFYDEALVPPAGHGWFGPILVAPQSRGQITLRSADPHAK PVIEPRYLSDLGGVDRAAMMAGLRICARIAQARPLRDLLGSIARPRNSTELDEATLELALATCSHTLYH PMGTCRMGSDEASWDPQLRVRGVDGLRVADASVMPSTVRGHTHAPSVLIGEKAADLIRS >Rv1294 thrA homoserine dehydrogenase TB.seq 1449373:1450695 MW:45522 SEQ ID NO:195

VPGDEKPVGVAVLGLGNVGSEWRIIENSAEDLAARVGAPLVLRGIGVRRVTTDRGVPIELLTDDIEEL VAREDVDIWEVMGPVEPSRKAILGALERGKSWTANKALLATSTGELAQAAESAHVDLYFEAAVAGA IPVIRPLTQSLAGDTVLRVAGIVNGTTNYILSAMDSTGADYASALADASALGYAEADPTADVEGYDAA AKAAILASIAFHTRVTADDVYREGITKVTPADFGSAHALGCTIKLLSICERITTDEGSQRVSARVYPALV PLSHPLAAVNGAFNAVWEAEAAGRLMFYGQGAGGAPTASAVTGDLVMAARNRVLGSRGPRESKY AQLPVAPMGFIETRYYVSMNVADKPGVLSAVAAEFAKREVSIAEVRQEGWDEGGRRVGARIVWTH LATDAALSETVDALDDLDWQGVSSVI RLEGTGL

>Rv1323 fadA4 acetyl-CoA C-acetyltransferase (aka thiL) TB.seq 1485860:1487026 MW:40049 SEQ ID NO:196

VIVAGARTPIGKLMGSLKDFSASELGAIAIKGALEKANVPASLVEYVIMGQVLTAGAGQMPARQAAVA AGIGWDVPALTINKMCLSGIDAIALADQLIRAREFDVWAGGQESMTKAPHLLMNSRSGYKYGDVTVL DHMAYDGLHDVFTDQPMGALTEQRNDVDMFTRSEQDEYAAASHQKAAAAWKDGVFADEVIPVNIP QRTGDPLQFTEDEGIRANTTAAALAGLKPAFRGDGTITAGSASQISDGAAAWVMNQEKAQELGLTW LAEIGAHGWAGPDSTLQSQPANAINKALDREGISVDQLDWEINEAFAAVALASIRELGLNPQIVNVN GGAIAVGHPLGMSGTRITLHAALQLARRGSGVGVAALCGAGGQGDALILRAG

>Rv1389 gmk putative guanylate kinase TB.seq 1564399:1565022 MW:22064 SEQ ID NO: 197

VSVGEGPDTKPTARGQPAAVGRVWLSGPSAVGKSTWRCLRERIPNLHFSVSATTRAPRPGEVDG VDYHFIDPTRFQQLIDQGELLEWAEIHGGLHRSGTLAQPVRAAAATGVPVLIEVDLAGARAIKKTMPE AVTVFLAPPSWQDLQARLIGRGTETADVIQRRLDTARIELAAQGDFDKWVNRRLESACAELVSLLVG TAPGSP

>Rv1407 fmu similar to Fmu protein TB.seq 1583099:1584469 MW:48494 SEQ ID NO:198

MTPRSRGPRRRPLDPARRAAFETLRAVSARDAYANLVLPALLAQRGIGGRDAAFATELTYGTCRAR GLLDAVIGAAAERSPQAIDPVLLDLLRLGTYQLLRTRVDAHAAVSTTVEQAGIEFDSARAGFVNGVLR TIAGRDERSWVGELAPDAQNDPIGHAAFVHAHPRWIAQAFADALGAAVGELEAVLASDDERPAVHLA ARPGVLTAGELARAVRGTVGRYSPFAVYLPRGDPGRLAPVRDGQALVQDEGSQLVARALTLAPVDG DTGRWLDLCAGPGGKTALLAGLGLQCAARVTAVEPSPHRADLVAQNTRGLPVELLRVDGRHTDLDP GFDRVLVDAPCTGLGALRRRPEARWRRQPADVAALAKLQRELLSAAIALTRPGGWLYATCSPHLAE TVGAVADALRRHPVHALDTRPLFEPVIAGLGEGPHVQLWPHRHGTDAMFAAALRRLT

>Rv1409 ribG riboflavin biosynthesis TB.seq 1585192:1586208 MW:35367 SEQ ID NO:199 MNVEQVKSIDEAMGLAIEHSYQVKGTTYPKPPVGAVIVDPNGRIVGAGGTEPAGGDHAEWALRRAG GLAAGAIVWTMEPCNHYGKTPPCVNALIEARVGTWYAVADPNGIAGGGAGRLSAAGLQVRSGVLA EQVAAGPLREWLHKQRTGLPHVTWKYATSIDGRSAAADGSSQWISSEAARLDLHRRRAIADAILVGT GTVLADDPALTARLADGSLAPQQPLRWVGKRDIPPEARVLNDEARTMMIRTHEPMEVLRALSDRTD

VLLEGGPTLAGAFLRAGAINRILAYVAPILLGGPVTAVDDVGVSNITNALRWQFDSVEKVGPDLLLSLV AR >Rv1440 secG TB.seq 1617715:1618065 MW:12140 SEQ ID NO:200

VAGVTAAVSARLKADEARRPGFYAAGSGPLPQVRGSTLPVMELALQITLIVTSVLWLLVLLHRAKGG GLSTLFGGGVQSSLSGSTWEKNLDRLTLFVTGIWLVSIIGVALLIKYR

>Rv1484 inhA TB.seq 1674200:1675006 MW:28529 SEQ ID NO:201

MTGLLDGKRILVSGIITDSSIAFHIARVAQEQGAQLVLTGFDRLRLIQRITDRLPAKAPLLELDVQNEEH LASLAGRVTEAIGAGNKLDGWHSIGFMPQTGMGINPFFDAPYADVSKGIHISAYSYASMAKALLPIM NPGGSIVGMDFDPSRAMPAYNWMTVAKSALESVNRFVAREAGKYGVRSNLVAAGPIRTLAMSAIVG GALGEEAGAQIQLLEEGWDQRAPIGWNMKDATPVAKTVCALLSDWLPATTGDIIYADGGAHTQLL

>Rv1617 pykA pyruvate kinase TB.seq 1816187:1817602 MW:50668 SEQ ID NO:202

VTRRGKIVCTLGPATQRDDLVRALVEAGMDVARMNFSHGDYDDHKVAYERVRVASDATGRAVGVL ADLQGPKIRLGRFASGATHWAEGETVRITVGACEGSHDRVSTTYKRLAQDAVAGDRVLVDDGKVAL WDAVEGDDWCTWEGGPVSDNKGISLPGMNVTAPALSEKDIEDLTFALNLGVDMVALSFVRSPAD VELVHEVMDRIGRRVPVIAKLEKPEAIDNLEAIVLAFDAVMVARGDLGVELPLEEVPLVQKRAIQMARE NAKPVIVATQMLDSMIENSRPTRAEASDVANAVLDGADALMLSGETSVGKYPLAAVRTMSRIICAVEE NSTAAPPLTHIPRTKRGVISYAARDIGERLDAKALVAFTQSGDTVRRLARLHTPLPLLAFTAWPEVRS QLAMTWGTETFIVPKMQSTDGMIRQVDKSLLELARYKRGDLWIVAGAPPGTVGSTNLIHVHRIGEDD

V

>Rv1630 rpsA 30S ribosomal protein S1 TB.seq 1833540:1834982 MW:53203 SEQ ID NO:203

MPSPTVTSPQVAVNDIGSSEDFLAAIDKTIKYFNDGDIVEGTIVKVDRDEVLLDIGYKTEGVIPARELSIK HDVDPNEWSVGDEVEALVLTKEDKEGRLILSKKRAQYERAWGTIEALKEKDEAVKGTVIEWKGGLI LDIGLRGFLPASLVEMRRVRDLQPYIGKEIEAKIIELDKNRNNWLSRRAWLEQTQSEVRSEFLNNLQK GTIRKGWSSIVNFGAFVDLGGVDGLVHVSELSWKHIDHPSEWQVGDEVTVEVLDVDMDRERVSLS LKATQEDPWRHFARTHAIGQIVPGKVTKLVPFGAFVRVEEGIEGLVHISELAERHVEVPDQWAVGDD AMVKVIDIDLERRRISLSLKQANEDYTEEFDPAKYGMADSYDEQGNYIFPEGFDAETNEWLEGFEKQ RAEWEARYAEAERRHKMHTAQMEKFAAAEAAGRGADDQSSASSAPSEKTAGGSLASDAQLAALRE KLAGSA

>Rv1631 - TB.seq 1835011:1836231 MW:44669 SEQ ID NO:204

MLRIGLTGGIGAGKSLLSTTFSQCGGIWDGDVLAREWQPGTEGLASLVDAFGRDILLADGALDRQA LAAKAFRDDESRGVLNGIVHPLVARRRSEIIAAVSGDAVWEDIPLLVESGMAPLFPLXΛΛΛ HADVELR VRRLVEQRGMAEADARARIAAQASDQQRRAVADNA /LDNSGSPEDLVRRARDVWNTRVQPFAHNL AQRQIARAPARLVPADPSWPDQARRIVNRLKIACGHKALRVDHIGSTAVSGFPDFLAKDVIDIQVTVE SLDVADELAEPLLAAGYPRLEHITQDTEKTDARSTVGRYDHTDSAALWHKRVHASADPGRPTNVHLR VHGWPNQQFALLFVDWLAANPGAREDYLTVKCDADRRADGELARYVTAKEPWFLDAYQRAWEWA

DAVHWRP

>Rv1706c - TB.seq 1932695:1933876 MW:39779 SEQ ID NO:205 MTLDVPVNQGHVPPGSVACCLVGVTAVADGIAGHSLSNFGALPPEINSGRMYSGPGSGPLMAAAAA WDGLAAELSSAATGYGAAISELTNMRWWSGPASDSMVAAVLPFVGWLSTTATLAEQAAMQARAAA AAFEAAFAMTVPPPAIAANRTLLMTLVDTNWFGQNTPAIATTESQYAEMWAQDAAAMYGYASAAAP ATVLTPFAPPPQTTNATGLVGHATAVAALRGQHSWAAAIPWSDIQKYWMMFLGALATAEGFIYDSG GLTLNALQFVGGMLWSTALAEAGAAEAAAGAGGAAGWSAWSQLGAGPVAASATLAAKIGPMSVPP GWSAPPATPQAQTVARSIPGIRSAAEAAETSVLLRGAPTPGRSRAAHMGRRYGRRLTVMADRPNVG

>Rv1745c - similar to Q46822 ORF_0182 TB.seq 1971381 :1971989 MW:22490 SEQ I D NO:206

MTRSYRPAPPIERWLLNDRGDATGVADKATVHTGDTPLHLAFSSYVFDLHDQLLITRRAATKRTWP AVWTNSCCGHPLPGESLPGAIRRRLAAELGLTPDRVDLILPGFRYRAAMADGTVENEICPVYRVQVD QQPRPNSDEVDAIRWLSWEQFVRDVTAGVIAPVSPWCRSQLGYLTKLGPCPAQWPVADDCRLPKA AHGN

>Rv1800 - TB.seq 2039451:2041415 MW:67068 SEQ ID NO:207

MLPNFAVLPPEVNSARVFAGAGSAPMLAAAAAWDDLASELHCAAMSFGSVTSGLWGWWQGSASA AMVDAAASYIGWLSTSAAHAEGAAGLARAAVSVFEEALAATVHPAMVAANRAQVASLVASNLFGQN APAIAALESLYECMWAQDAAAMAGYYVGASAVATQLASWLQRLQSIPGAASLDARLPSSAEAPMGV VRAVNSAIAANAAAAQTVGLVMGGSGTPIPSARYVELANALYMSGSVPGVIAQALFTPQGLYPVWIK NLTFDSSVAQGAVILESAIRQQIAAGNNVTVFGYSQSATISSLVMANLAASADPPSPDELSFTLIGNPN NPNGGVATRFPGISFPSLGVTATGATPHNLYPTKIYTIEYDGVADFPRYPLNFVSTLNAIAGTYYVHSN YFILTPEQIDAAVPLTNTVGPTMTQYΎIIRTENLPLLEPLRSVPIVGNPLANLVQPNLKVIVNLGYGDPA YGYSTSPPNVATPFGLFPEVSPWIADALVAGTQQGIGDFAYDVSHLELPLPADGSTMPSTAPGSGT PVPPLSIDSLIDDLQVANRNLANTISKVAATSYATVLPTADIANAALTIVPSYNIHLFLEGIQQALKGDPM GLVNAVGYPLAADVALFTAAGGLQLLIIISAGRTIANDISAIVP

>Rv1844c gnd 6-phosphogluconate dehydrogenase (Gram -) TB.seq 2093732:2095186

MW:51548 SEQ ID NO:208

MSSSESPAGIAQIGVTGLAVMGSNIARNFARHGYTVAVHNRSVAKTDALLKEHSSDGKFVRSETIPEF LAALEKPRRVLIMVKAGEATDADAVINELADAMEPGDIIIDGGNALYTDTMRREKAMRERGLHFVGAG ISGGEEGALNGPSIMPGGPAESYQSLGPLLEEISAHVDGVPCCTHIGPDGSGHFVKMVHNGIEYSDM QLIGEAYQLMRDGLGLTAPAIADVFTEWNNGDLDSYLVEITAEVLRQTDAKTGKPLVDVIVDRAEQKG TGRWTVKSALDLGVPVTGIAEAVFARALSGSVGQRSAASGLASGKLGEQPADPATFTEDVRQALYA SKIVAYAQGFNQIQAGSAEFGWDITPGDLATIWRGGCIIRAKFLNHIKEAFDASPNLASLIVAPYFRGA VESAIDSWRRWSTAAQLGIPTPGFSSALSYYDALRTARLPAALTQAQRDFFGAHTYGRIDEPGKFHT LWSSDRTEVPV

>Rv1900c lipJ TB.seq 2146246:2147631 MW:49685 SEQ ID NO:209 VAQAPHIHRTRYAKCGDMDIAYQVLGDGPTDLLVLPGPFVPIDSIDDEPSLYRFHRRLASFSRVIRLDH RGVGLSSRLAAITTLGPKFWAQDAIAVMDAVGCEQATIFAPSFHAMNGLVLAADYPERVRSLIWNGS ARPLWAPDYPVGAQVRRADPFLTVALEPDAVERGFDVLSIVAPTVAGDDVFRAWWDLAGNRAGPP SIARAVSKVIAEADVRDVLGHIEAPTLILHRVGSTYIPVGHGRYLAEHIAGSRLVELPGTDTLYWVGDT GPMLDEIEEFITGVRGGADAERMLATIMFTDIVGSTQHAAALGDDRWRDLLDNHDTIVCHEIQRFGGR EVNTAGDGFVATFTSPSAAIACADDIVDAVAALGIEVRIGIHAGEVEVRDASHGTDVAGVAVHIGARVC ALAGPSEVLVSSTVRDIVAGSRHRFAERGEQELKGVPGRWRLCVLMRDDATRTR

>Rv1967 - TB.seq 2210599:2211624 MW:36516 SEQ ID NO:210

MRENLGGVWRLGVFLAVCLLTAFLLIAVFGEVRFGDGKTYYAEFANVSNLRTGKLVRIAGVEVGKVT RISINPDATVRVQFTADNSVTLTRGTRAVIRYDNLFGDRYLALEEGAGGLAVLRPGHTIPLARTQPALD LDALIGGFKPLFRALNPEQVNALSEQLLHAFAGQGPTIGSLLAQSAAVTNTLADRDRLIGQVITNLNW LGSLGAHTDRLDQAVTSLSALIHRLAQRKTDISNAVAYTNAAAGSVADLLSQARAPLAKWRETDRVA GIAAADHDYLDNLLNTLPDKYQALVRQGMYGDFFAFYLCDWLKVNGKGGQPVYIKLAGQDSGRCA

PK

>Rv1975 - TB.seq 2218050:2218712 MW:23650 SEQ ID N0:211

MSRRASATCALSATTAVAIMAAPAARADDKRLNDGWANVYTVQRQAGCTNDVTINPQLQLAAQWH TLDLLNNRHLNDDTGSDGSTPQDRAHAAGFRGKVAETVAINPAVAISGIELINQWYYNPAFFAIMSDC ANTQIGVWSENSPDRTVWAVYGQPDRPSAMPPRGAVTGPPSPVAAQENVPIDPSPDYDASDEIEY GINWLPWILRGVYPPPAMPPQ

>Rv1981c nrdF ribonucleotide reductase small subunit TB.seq 2224221:2225186 MW:36591 SEQ ID NO:212

MTGKLVERVHAINWNRLLDAKDLQVWERLTGNFWLPEKIPLSNDLASWQTLSSTEQQTTIRVFTGLT LLDTAQATVGAVAMIDDAVTPHEEAVLTNMAFMESVHAKSYSSIFSTLCSTKQIDDAFDWSEQNPYL QRKAQIIVDYΎRGDDALKRKASSVMLESFLFYSGFYLPMYWSSRGKLTNTADLIRLIIRDEAVHGYYIG YKCQRGLADLTDAERADHREYTCELLHTLYANEIDYAHDLYDELGWTDDVLPYMRYNANKALANLG YQPAFDRDTCQVNPAVRAALDPGAGENHDFFSGSGSSYVMGTHQPTTDTDWDF

>Rv2092c helY helicase, Ski2 subfamily TB.seq 2349335:2352052 MW:99576 SEQ ID NO:213

VTELAELDRFTAELPFSLDDFQQRACSALERGHGVLVCAPTGAGKTWGEFAVHLALAAGSKCFYTT PLKALSNQKHTDLTARYGRDQIGLLTGDLSVNGNAPWVMTTEVLRNMLYADSPALQGLSYWMDE VHFLADRMRGPNΛΛ/EEVILQLPDDVRVVSLSATVSNAEEFGGWIQTVRGDTTVVVDEHRPVPLWQHV LVGKRMFDLFDYRIGEAEGQPQVNRELLRHIAHRREADRMADWQPRRRGSGRPGFYRPPGRPEVI AKLDAEGLLPAITFVFSRAGCDAAVTQCLRSPLRLTSEEERARIAEVIDHRCGDLADSDLAVLGYYEW REGLLRGLAAHHAGMLPAFRHTVEELFTAGLVKAVFATETLALGINMPARTWLERLVKFNGEQHMP LTPGEYTQLTGRAGRRGIDVEGHAWIWHPEIEPSEVAGLASTRTFPLRSSFAPSYNMTINLVHRMGP QQAHRLLEQSFAQYQADRSWGLVRGIERGNRILGEIAAELGGSDAPILEYARLRARVSELERAQARA SRLQRRQAATDALAALRRGDIITITHGRRGGLAWLESARDRDDPRPLVLTEHRWAGRISSADYSGTT PVGSMTLPKRVEHRQPRVRRDLASALRSAAAGLVIPAARRVSEAGGFHDPELESSREQLRRHPVHT SPGLEDQIRQAERYLRIERDNAQLERKVAAATNSLARTFDRFVGLLTEREFIDGPATDPWTDDGRLL ARIYSESDLLVAECLRTGAWEGLKPAELAGWSAWYETRGGDGQGAPFGADVPTPRLRQALTQTS RLSTTLRADEQAHRITPSREPDDGFVRVIYRWSRTGDLAAALAAADVNGSGSPLLAGDFVRWCRQV LDLLDQVRNAAPNPELRATAKRAIGDIRRGWAVDAG

>Rv2101 helZ helicase, Snf2/Rad54 family TB.seq 2360238:2363276 MW:111632 SEQ ID NO:214

MLVLHGFWSNSGGMRLWAEDSDLLVKSPSQALRSARPHPFAAPADLIAGIHPGKPATAVLLLPSLRS APLDSPELIRLAPRPAARTDPMLLAWTVPWDLDPTAALAAFDQPAPDVRYGASVDYLAELAVFAREL VERGRVLPQLRRDTHGAAACWRPVLQGRDWAMTSLVSAMPPVCRAEVGGHDPHELATSALDAMV DAAVRAALSPMDLLPPRRGRSKRHRAVEAWLTALTCPDGRFDAEPDELDALAEALRPWDDVGIGTV GPARATFRLSEVETENEETPAGSLWRLEFLLQSTQDPSLLVPAEQAWNDDGSLRRWLDRPQELLLT ELGRASRIFPELVPALRTACPSGLELDADGAYRFLSGTAAVLDEAGFGVLLPSWWDRRRKLGLVLSA YTPVDGWGKASKFGREQLVEFRWELAVGDDPLSEEEIAALTETKSPLIRLRGQWVALDTEQMRRGL EFLERKPTGRKTTAEILALAASHPDDVDTPLEVTAVRADGWLGDLLAGAAAASLQPLDPPDGFTATLR PYQQRGLAWLAFLSSLGLGSCLADDMGLGKTVQLLALETLESVQRHQDRGVGPTLLLCPMSLVGN WPQEAARFAPNLRVYAHHGGARLHGEALRDHLERTDLWSTYTTATRDIDELAEYEWNRWLDEAQ AVKNSLSRAAKAVRRLRAAHRVALTGTPMENRLAELWSIMDFLNPGLLGSSERFRTRYAIPIERHGHT EPAERLRASTRPYILRRLKTDPAIIDDLPEKIEIKQYCQLTTEQASLYQAWADMMEKIENTEGIERRGN VLAAMAKLKQVCNHPAQLLHDRSPVGRRSGKVIRLEEILEEILAEGDRVLCFTQFTEFAELLVPHLAAR FGRAARDIAYLHGGTPRKRRDEMVARFQSGDGPPIFLLSLKAGGTGLNLTAANHWHLDRWWNPAV ENQATDRAFRIGQRRTVQVRKFICTGTLEEKIDEMIEEKKALADLWTDGEGWLTELSTRDLREVFAL

SEGAVGE

>Rv2110c prcB proteasome [beta]-type subunit 2 TB.seq 2369727:2370599 MW:30274 SEQ ID NO:215 VTWPLPDRLSINSLSGTPAVDLSSFTDFLRRQAPELLPASISGGAPLAGGDAQLPHGTTIVALKYPGG WMAGDRRSTQGNMISGRDVRKVYITDDYTATGIAGTAAVAVEFARLYAVELEHYEKLEGVPLTFAG KINRLAIMVRGNLAAAMQGLLALPLLAGYDIHASDPQSAGRIVSFDAAGGWNIEEEGYQAVGSGSLFA KSSMKKLYSQVTDGDSGLRVAVEALYDAADDDSATGGPDLVRGIFPTAVIIDADGAVDVPESRIAELA RAI I ESRSGADTFGSDGGEK

>Rv2118c - = B2126_C1_165 (83.6%) TB.seq 2377471:2378310 MW:30091 SEQ ID NO:216

VSATGPFSIGERVQLTDAKGRRYTMSLTPGAEFHTHRGSIAHDAVIGLEQGSWKSSNGALFLVLRPL

LVDYVMSMPRGPQVIYPKDAAQIVHEGDIFPGARVLEAGAGSGALTLSLLRAVGPAGQVISYEQRAD

HAEHARRNVSGCYGQPPDNWRLWSDLADSELPDGSVDRAVLDMLAPWEVLDAVSRLLVAGGVLM

VYVATVTQLSRIVEALRAKQCWTEPRAWETLQRGWNWGLAVRPQHSMRGHTAFLVATRRLAPGA

VAPAPLGRKREGRDG

>Rv2144c - TB.seq 2404166:2404519 MW:12028 SEQ ID NO:217

MLIIALVLALIGLLALVFAWTSNQLVAWVCIGASVLGVALLIVDALRERQQGGADEADGAGETGVAEE ADVDYPEEAPEESQAVDAGVIGSEEPSEEASEATEESAVSADRSDDSAK

>Rv2146c - TB.seq 2405667:2405954 MW:10805 SEQ ID NO:218

LWFFQI LGFALFI FWLLLI ARVWEFI RSFSRDWRPTGVTWI LEI I MSITDPPVKVLRRLI PQLTIGAVRF DLSIMVLLLVAFIGMQLAFGAAA

>Rv2147c - TB.seq 2406119:2406841 MW:27630 SEQ ID NO:219 VNSHCSHTFITDNRSPRARRGHAMSTLHKVKAYFGMAPMEDYDDEYYDDRAPSRGYARPRFDDDY GRYDGRDYDDARSDSRGDLRGEPADYPPPGYRGGYADEPRFRPREFDRAEMTRPRFGSWLRNST RGALAMDPRRMAMMFEDGHPLSKITTLRPKDYSEARTIGERFRDGSPVIMDLVSMDNADAKRLVDF AAGLAFALRGSFDKVATKVFLLSPADVDVSPEERRRIAETGFYAYQ

>Rv2148c - TB.seq 2406841:2407614 MW:27694 SEQ ID NO:220

MAADLSAYPDRESELTHALAAMRSRLAAAAEAAGRNVGEIELLPITKFFPATDVAILFRLGCRSVGES REQEASAKMAELNRLLAAAELGHSGGVHWHMVGRIQRNKAGSLARWAHTAHSVDSSRLVTALDRA WAALAEHRRGERLRVYVQVSLDGDGSRGGVDSTTPGAVDRICAQVQESEGLELVGLMGIPPLDWD PDEAFDRLQSEHNRVRAMFPHAIGLSAGMSNDLEVAVKHGSTCVRVGTALLGPRRLRSP

>Rv2150c ftsZ TB.seq 2408386:2409522 MW:38757 SEQ ID NO:221

MTPPHNYLAVIKWGIGGGGVNAVNRMIEQGLKGVEFIAINTDAQALLMSDADVKLDVGRDSTRGLG AGADPEVGRKAAEDAKDEIEELLRGADMVFVTAGEGGGTGTGGAPWASIARKLGALTVGWTRPF SFEGKRRSNQAENGIAALRESCDTLIVIPNDRLLQMGDAAVSLMDAFRSADEVLLNGVQGITDLITTP GLINVDFADVKGIMSGAGTALMGIGSARGEGRSLKAAEIAINSPLLEASMEGAQGVLMSIAGGSDLGL FEINEAASLVQDAAHPDANIIFGTVIDDSLGDEVRVTVIAAGFDVSGPGRKPVMGETGGAHRIESAKA GKLTSTLFEPVDAVSVPLHTNGATLSIGGDDDDVDVPPFMRR >Rv2152c murC TB.seq 2410639:2412120 MW:51146 SEQ ID NO:222

VSTEQLPPDLRRVHMVGIGGAGMSGIARILLDRGGLVSGSDAKESRGVHALRARGALIRIGHDASSL DLLPGGATAWTTHAAIPKTNPELVEARRRGIPWLRPAVLAKLMAGRTTLMVTGTHGKTTTTSMLIVA LQHCGLDPSFAVGGELGEAGTNAHHGSGDCFVAEADESDGSLLQYTPHVAVITNIESDHLDFYGSVE AYVAVFDSFVERIVPGGALWCTDDPGGAALAQRATELGIRVLRYGSVPGETMAATLVSWQQQGVG AVAHIRLASELATAQGPRVMRLSVPGRHMALNALGALLAAVQIGAPADEVLDGLAGFEGVRRRFELV GTCGVGKASVRVFDDYAHHPTEISATLAAARMVLEQGDGGRCMWFQPHLYSRTKAFAAEFGRALN AADEVFVLDVYGAREQPLAGVSGASVAEHVTVPMRYVPDFSAVAQQVAAAASPGDVIVTMGAGDVT LLGPEILTALRVRANRSAPGRPGVLG

>Rv2153c murG TB.seq 2412120:2413349 MW:41829 SEQ ID NO:223

VKDTVSQPAGGRGATAPRPADAASPSCGSSPSADSVSWLAGGGTAGHVEPAMAVADALVALDPR VRITALGTLRGLETRLVPQRGYHLELITAVPMPRKPGGDLARLPSRVWRAVREARDVLDDVDADVW GFGGYVALPAYLAARGLPLPPRRRRRIPWIHEANARAGLANRVGAHTADRVLSAVPDSGLRRAEW GVPVRASIAALDRAVLRAEARAHFGFPDDARVLLVFGGSQGAVSLNRAVSGAAADLAAAGVCVLHA HGPQNVLELRRRAQGDPPYVAVPYLDRMELAYAAADLVICRAGAMTVAEVSAVGLPAIYVPLPIGNG EQRLNALPWNAGGGMWADAALTPELVARQVAGLLTDPARLAAMTAAAARVGHRDAAGQVARAAL AVATGAGARTTT

>Rv2154c ftsW TB.seq 2413349:2414920 MW:56306 SEQ ID NO:224

VLTRLLRRGTSDTDGSQTRGAEPVEGQRTGPEEASNPGSARPRTRFGAWLGRPMTSFHLIIAVAALL TTLGLIMVLSASAVRSYDDDGSAWVIFGKQVLWTLVGLIGGYVCLRMSVRFMRRIAFSGFAITIVMLVL VLVPGIGKEANGSRGWFWAGFSMQPSELAKMAFAIWGAHLLAARRMERASLREMLIPLVPAAWAL ALIVAQPDLGQTVSMGIILLGLLWYAGLPLRVFLSSLAAVWSAAILAVSAGYRSDRVRSWLNPENDP QDSGYQARQAKFALAQGGIFGDGLGQGVAKWNYLPNAHNDFIFAIIGEELGLVGALGLLGLFGLFAY TGMRIASRSADPFLRLLTATTTLWVLGQAFINIGYVIGLLPVTGLQLPLISAGGTSTAATLSLIGIIANAAR HEPEAVAALRAGRDDKVNRLLRLPLPEPYLPPRLEAFRDRKRANPQPAQTQPARKTPRTAPGQPAR QMGLPPRPGSPRTADPPVRRSVHHGAGQRYAGQRRTRRVRALEGQRYG

>Rv2155c murD TB.seq 2414935:2416392 MW:49314 SEQ ID NO:225

VLDPLGPGAPVLVAGGRVTGQAVAAVLTRFGATPTVCDDDPVMLRPHAERGLPTVSSSDAVQQITG YALWASPGFSPATPLLAAAAAAGVPIWGDVELAWRLDAAGCYGPPRSWLWTGTNGKTTTTSMLH AMLIAGGRRAVLCGNIGSAVLDVLDEPAELLAVELSSFQLHWAPSLRPEAGAVLNIAEDHLDWHATM AEYTAAKARVLTGGVAVAGLDDSRAAALLDGSPAQVRVGFRLGEPAARELGVRDAHLVDRAFSDDL TLLPVASIPVPGPVGVLDALAAAALARSVGVPAGAIADAVTSFRVGRHRAEWAVADGITYVDDSKAT NPHAARASVLAYPRWWIAGGLLKGASLHAEVAAMASRLVGAVLIGRDRAAVAEALSRHAPDVPWQ WAGEDTGMPATVEVPVACVLDVAKDDKAGETVGAAVMTAAVAAARRMAQPGDTVLLAPAGASFD QFTGYADRGEAFATAVRAVI R

>Rv2156c murX TB.seq 2416397:2417473 MW:37714 SEQ ID NO:226 MRQILIAVAVAVTVSILLTPVLIRLFTKQGFGHQIREDGPPSHHTKRGTPSMGGVAILAGIWAGYLGAH LAGLAFDGEGIGASGLLVLGLATALGGVGFIDDLIKIRRSRNLGLNKTAKTVGQITSAVLFGVLVLQFRN AAGLTPGSADLSYVREIATVTLAPVLFVLFCWIVSAWSNAVNFTDGLDGLAAGTMAMVTAAYVLITF WQYRNACVTAPGLGCYNVRDPLDLALIAAATAGACIGFLWWNAAPAKIFMGDTGSLALGGVIAGLSV TSRTEILAWLGALFVAEITSWLQILTFRTTGRRMFRMAPFHHHFELVGWAETTVIIRFWLLTAITCGL GVALFYGEWLAAVGA

>Rv2157c murF TB.seq 2417473:2419002 MW:51634 SEQ ID NO:227

MIELTVAQIAEIVGGAVADISPQDAAHRRVTGTVEFDSRAIGPGGLFLALPGARADGHDHAASAVAAG AAWLAARPVGVPAIWPPVAAPNVLAGVLEHDNDGSGAAVLAALAKLATAVAAQLVAGGLTIIGITGS SGKTSTKDLMAAVLAPLGEWAPPGSFNNELGHPWTVLRATRRTDYLILEMAARHHGNIAALAEIAPP SIGWLNVGTAHLGEFGSREVIAQTKAELPQAVPHSGAWLNADDPAVAAMAKLTAARWRVSRDNT GDVWAGPVSLDELARPRFTLHAHDAQAEVRLGVCGDHQVTNALCAAAVALECGASVEQVAAALTAA PPVSRHRMQVTTRGDGVTVIDDAYNANPDSMRAGLQALAWIAHQPEATRRSWAVLGEMAELGEDAI AEHDRIGRLAVRLDVSRLVWGTGRSISAMHHGAVLEGAWGSGEATADHGADRTAVNVADGDAALA LLRAELRPGDWLVKASNAAGLGAVADALVADDTCGSVRP

>Rv2158c murE TB.seq 2419002:2420606 MW:55310 SEQ ID NO:228

VSSLARGISRRRTEVATQVEAAPTGLRPNAWGVRLAALADQVGAALAEGPAQRAVTEDRTVTGVTL RAQDVSPGDLFAALTGSTTHGARHVGDAIARGAVAVLTDPAGVAEIAGRAAVPVLVHPAPRGVLGGL AATVYGHPSERLTVIGITGTSGKTTTTYLVEAGLRAAGRVAGLIGTIGIRVGGADLPSALTTPEAPTLQA MLAAMVERGVDTWMEVSSHALALGRVDGTRFAVGAFTNLSRDHLDFHPSMADYFEAKASLFDPDS ALRARTAWCIDDDAGRAMAARAADAITVSAADRPAHWRATDVAPTDAGGQQFTAIDPAGVGHHIGI RLPGRYNVANCLVALAILDTVGVSPEQAVPGLREIRVPGRLEQIDRGQGFLALVDYAHKPEALRSVLT TLAHPDRRLAWFGAGGDRDPGKRAPMGRIAAQLADLVWTDDNPRDEDPTAIRREILAGAAEVGGD AQWEIADRRDAIRHAVAWARPGDWLIAGKGHETGQRGGGRVRPFDDRVELAAALEALERRA

>Rv2159c - TB.seq 2420632:2421663 MW:36377 SEQ ID NO:229

MKFVNHIEPVAPRRAGGAVAEVYAEARREFGRLPEPLAMLSPDEGLLTAGWATLRETLLVGQVPRG RKEAVAAAVAASLRCPWCVDAHTTMLYAAGQTDTAAAILAGTAPAAGDPNAPYVAWAAGTGTPAGP PAPFGPDVAAEYLGTAVQFHFIARLVLVLLDETFLPGGPRAQQLMRRAGGLVFARKVRAEHRPGRST RRLEPRTLPDDLAWATPSEPIATAFAALSHHLDTAPHLPPPTRQWRRWGSWHGEPMPMSSRWTN EHTAELPADLHAPTRLALLTGLAPHQVTDDDVAAARSLLDTDAALVGALAWAAFTAARRIGTWIGAAA EGQVSRQNPTG

>Rv2163c pbpB TB.seq 2425049:2427085 MW:72506 SEQ ID NO:230 VSRAAPRRASQSQSTRPARGLRRPPGAQEVGQRKRPGKTQKARQAQEATKSRPATRSDVAPAGR STRARRTRQWDVGTRGASFVFRHRTGNAVILVLMLVAATQLFFLQVSHAAGLRAQAAGQLKVTDV QPAARGSIVDRNNDRLAFTIEARALTFQPKRIRRQLEEARKKTSAAPDPQQRLRDIAQEVAGKLNNKP DAAAVLKKLQSDETFVYLARAVDPAVASAICAKYPEVGAERQDLRQYPGGSLAANWGGIDWDGHG LLGLEDSLDAVLAGTDGSVTYDRGSDGWIPGSYRNRHKAVHGSTWLTLDNDIQFYVQQQVQQAK NLSGAHNVSAWLDAKTGEVLAMANDNTFDPSQDIGRQGDKQLGNPAVSSPFEPGSVNKIVAASAVI EHGLSSPDEVLQVPGSIQMGGVTVHDAWEHGVMPYTTTGVFGKSSNVGTLMLSQRVGPERYYDML RKFGLGQRTGVGLPGESAGLVPPIDQWSGSTFANLPIGQGLSMTLLQMTGMYQAIANDGVRVPPRII KATVAPDGSRTEEPRPDDIRWSAQTAQTVRQMLRAWQRDPMGYQQGTGPTAGVPGYQMAGKT GTAQQINPGCGCYFDDVYWITFAGIATADNPRYVIGIMLDNPARNSDGAPGHSAAPLFHNIAGWLMQ RENVPLSPDPGPPLVLQAT

>Rv2165c - TB.seq 2428236:2429423 MW:42498 SEQ ID NO:231

VQTRAPWSLPEATLAYFPNARFVSSDRDLGAGAAPGIAASRSTACQTWGGITVADPGSGPTGFGHV PVLAQRCFELLTPALTRYYPDGSQAVLLDATIGAGGHAERFLEGLPGLRLIGLDRDPTALDVARSRLV RFADRLTLVHTRYDCLGAALAESGYAAVGSVDGILFDLGVSSMQLDRAERGFAYATDAPLDMRMDP TTPLTAADIVNTYDEAALADILRRYGEERFARRIAAGIVRRRAKTPFTSTAELVALLYQAIPAPARRVGG HPAKRTFQALRIAVNDELESLRTAVPAALDALAIGGRIAVLAYQSLEDRIVKRVFAEAVASATPAGLPV ELPGHEPRFRSLTHGAERASVAEIERNPRSTPVRLRALQRVEHRAQSQQWATEKGDS

>Rv2166c - TB.seq 2429428:2429856 MW:15912 SEQ ID NO:232

MFLGTYTPKLDDKGRLTLPAKFRDALAGGLMVTKSQDHSLAVYPRAAFEQLARRASKAPRSNPEAR

AFLRNLAAGTDEQHPDSQGRITLSADHRRYASLSKDCWIGAVDYLEIWDAQAWQNYQQIHEENFSA

ASDEALGDIF

>Rv2197c - TB.seq 2461505:2462146 MW:22481 SEQ ID NO:233 MVSRYSAYRRGPDVISPDVIDRILVGACAAVWLVFTGVSVAAAVALMDLGRGFHEMAGNPHTTWVL YAVIWSALVIVGAIPVLLRARRMAEAEPATRPTGASVRGGRSIGSGHPAKRAVAESAPVQHADAFEV AAEWSSEAVDRIWLRGTWLTSAIGIALIAVAAATYLMAVGHDGPSWISYGLAGWTAGMPVIEWLYA RQLRRWAPQSS

>Rv2198c - TB.seq 2462149:2463045 MW:30955 SEQ ID NO:234 MSGPNPPGREPDEPESEPVSDTGDERASGNHLPPVAGGGDKLPSDQTGETDAYSRAYSAPESEHV TGGPYVPADLRLYDYDDYEESSDLDDELAAPRWPWWGVAAIIAAVALWSVSLLVTRPHTSKLATG DTTSSAPPVQDEITTTKPAPPPPPPAPPPTTEIPTATETQTVTVTPPPPPPPATTTAPPPATTTTAAAP PPTTTTPTGPRQVTYSVTGTKAPGDIISVTYVDAAGRRRTQHNVYIPWSMTVTPISQSDVGSVEASSL FRVSKLNCSITTSDGTVLSSNSNDGPQTSC

>Rv2199c - TB.seq 2463234:2463650 MW:14866 SEQ ID NO:235 MHIEARLFEFVAAFFWTAVLYGVLTSMFATGGVEWAGTTALALTGGMALIVATFFRFVARRLDSRPE DYEGAEISDGAGELGFFSPHSWWPIMVALSGSVAAVGIALWLPWLIAAGVAFILASAAGLVFEYYVGP

EKH

>Rv2200c ctaC TB.seq 2463661:2464749 MW:40449 SEQ ID NO:236 VTPRGPGRLQRLSQCRPQRGSGGPARGLRQLALAAMLGALAVTVSGCSWSEALGIGWPEGITPEA HLNRELWIGAVIASLAVGVIVWGLIFWSAVFHRKKNTDTELPRQFGYNMPLELVLTVIPFLIISVLFYFT VWQEKMLQIAKDPEWIDITSFQWNWKFGYQRVNFKDGTLTYDGADPERKRAMVSKPEGKDKYGE ELVGPVRGLNTEDRTYLNFDKVETLGTSTEIPVLVLPSGKRIEFQMASADVIHAFWVPEFLFKRDVMP NPVANNSVNVFQIEEITKTGAFVGHCAEMCGTYHSMMNFEVRWTPNDFKAYLQQRIDGKTNAEALR AINQPPLAVTTHPFDTRRGELAPQPVG

>Rv2427c proA g-glutamyl phosphate reductase TB.seq 2724231:2725475 MW:43746

SEQ ID NO:237

MTVPAPSQLDLRQEVHDAARRARVAARRLASLPTTVKDRALHAAADELLAHRDQILAANAEDLNAAR EADTPAAMLDRLSLNPQRVDGIAAGLRQVAGLRDPVGEVLRGYTLPNGLQLRQQRVPLGWGMIYE GRPNVTVDAFGLTLKSGNAALLRGSSSAAKSNEALVAVLRTALVGLELPADAVQLLSAADRATVTHLI QARGLVDWIPRGGAGLIEAWRDAQVPTIETGVGNCHVYVHQAADLDVAERILLNSKTRRPSVCNA AETLLVDAAIAETALPRLLAALQHAGVTVHLDPDEADLRREYLSLDIAVAWDGVDAAIAHINEYGTGH TEAIVTTNLDAAQRFTEQIDAAAVMVNASTAFTDGEQFGFGAEIGISTQKLHARGPMGLPELTSTKWI AWGAGHTRPA

>Rv2438c - similar to YHN4_YEAST P38795 TB.seq 2734793:2737006 MW:80492 SEQ ID NO:238

MGLLGGQSGPRVGSGPVGSIPTPVNAAICQQRGGFHGVERGYSAGDSGVLTSLGDNERTMNFYSA YQHGFVRVAACTHHTTIGDPAANAASVLDMARACHDDGAALAVFPELTLSGYSIEDVLLQDSLLDAV EDALLDLVTESADLLPVLWGAPLRHRHRIYNTAWIHRGAVLGWPKSYLPTYREFYERRQMAPGD GERGTIRIGGADVAFGTDLLFAASDLPGFVLHVEICEDMFVPMPPSAEAALAGATVLANLSGSPITIGR AEDRRLLARSASARCLAAYVYAAAGEGESTTDLAWDGQTMIWENGALLAESERFPKGVRRSVADVD TELLRSERLRMGTFDDNRRHHRELTESFRRIDFALDPPAGDIGLLREVERFPFVPADPQRLQQDCYE AYNIQVSGLEQRLRALDYPKWIGVSGGLDSTHALIVATHAMDREGRPRSDILAFALPGFATGEHTKN NAIKLARALGVTFSEIDIGDTARLMLHTIGHPYSVGEKVYDVTFENVQAGLRTDYLFRIANQRGGIVLG TGDLSELALGWSTYGVGDQMSHYNVNAGVPKTLIQHLIRWVISAGEFGEKVGEVLQSVLDTEITPELI PTGEEELQSSEAKVGPFALQDFSLFQVLRYGFRPSKIAFLAWHAWNDAERGNWPPGFPKSERPSYS LAEIRHWLQIFVQRFYSFSQFKRSALPNGPKVSHGGALSPRGDWRAPSDMSARIWLDQIDREVPKG

>Rv2439c proB glutamate 5-kinase TB.seq 2737118:2738245 MW:38789 SEQ ID NO:239 MRSPHRDAIRTARGLWKVGTTALTTPSGMFDAGRLAGLAEAVERRMKAGSDWIVSSGAIAAGIEPL GLSRRPKDLATKQAAASVGQVALVNSWSAAFARYGRTVGQVLLTAHDISMRVQHTNAQRTLDRLRA LHAVAIVNENDTVATNEIRFGDNDRLSALVAHLVGADALVLLSDIDGLYDCDPRKTADATFIPEVSGPA DLDGWAGRSSHLGTGGMASKVAAALLAADAGVPVLLAPAADAATALADASVGTVFAARPARLSAR RFWVRYAAEATGALTLDAGAVRAWRQRRSLLAAGITAVSGRFCGGDWELRAPDAAMVARGWAY DASELATMVGRSTSELPGELRRPWHADDLVAVSAKQAKQV

>Rv2440c obg Obg GTP-binding protein TB.seq 2738248:2739684 MW:50430

SEQ ID NO:240

VPRFVDRWIHTRAGSGGNGCASVHREKFKPLGGPDGGNGGRGGSIVFWDPQVHTLLDFHFRPHL TAASGKHGMGNNRDGAAGADLEVKVPEGTWLDENGRLLADLVGAGTRFEAAAGGRGGLGNAALA SRVRKAPGFALLGEKGQSRDLTLELKTVADVGLVGFPSAGKSSLVSAISAAKPKIADYPFTTLVPNLG WSAGEHAFTVADVPGLIPGASRGRGLGLDFLRHIERCAVLVHWDCATAEPGRDPISDIDALETELA CYTPTLQGDAALGDLAARPRAWLNKIDVPEARELAEFVRDDIAQRGWPVFCVSTATRENLQPLIFGL SQMISDYNAARPVAVPRRPVIRPIPVDDSGFTVEPDGHGGFWSGARPERWIDQTNFDNDEAVGYL ADRLARLGVEEELLRLGARSGCAVTIGEMTFDWEPQTPAGEPVAMSGRGTDPRLDSNKRVGAAER KAARSRRREHGDG

>Rv2441c rpmA 50S ribosomal protein L27 TB.seq 2739773:2740030 MW:8969

SEQ ID NO:241 MAHKKGASSSRNGRDSAAQRLGVKRYGGQWKAGEILVRQRGTKFHPGVNVGRGGDDTLFAKTAG AVEFGIKRGRKTVSIVGSTTA

>Rv2442c rplU 50S ribosomal protein L21 TB.seq 2740048:2740359 MW:11152

SEQ ID NO:242 MMATYAIVKTGGKQYKVAVGDWKVEKLESEQGEKVSLPVALWDGATVTTDAKALAKVAVTGEVLG

HTKGPKIRIHKFKNKTGYHKRQGHRQQLTVLKVTGIA

>Rv2448c valS valyl-tRNA synthase TB.seq 2747596:2750223 MW:97822 SEQ ID NO:243

MLPKSWDPAAMESAIYQKWLDAGYFTADPTSTKPAYSIVLPPPNVTGSLHMGHALEHTMMDALTRR KRMQGYEVLWQPGTDHAGIATQSWEQQLAVDGKTKEDLGRELFVDKVWDWKRESGGAIGGQMR

RLGDGVDWSRDRFTMDEGLSRAVRTIFKRLYDAGLIYRAERLVNWSPVLQTAISDLEVNYRDVEGEL

VSFRYGSLDDSQPHIWATTRVETMLGDTAIAVHPDDERYRHLVGTSLAHPFVDRELAIVADEHVDPE FGTGAVKVTPAHDPNDFEIGVRHQLPMPSILDTKGRIVDTGTRFDGMDRFEARVAVRQALAAQGRV VEEKRPYLHSVGHSERSGEPIEPRLSLQWWVRVESLAKAAGDAVRNGDTVIHPASMEPRWFSWVD DMHDWCISRQLWWGHRIPIWYGPDGEQVCVGPDETPPQGWEQDPDVLDTWFSSALWPFSTLGW PDKTAELEKFYPTSVLVTGYDILFFWVARMMMFGTFVGDDAAITLDGRRGPQVPFTDVFLHGLIRDE SGRKMSKSKGNVIDPLDWVEMFGADALRFTLARGASPGGDLAVSEDAVRASRNFGTKLFNATRYAL LNGAAPAPLPSPNELTDADRWILGRLEEVRAEVDSAFDGYEFSRACESLYHFAWDEFCDWYLELAK TQLAQGLTHTTAVLAAGLDTLLRLLHPVIPFLTEALWLALTGRESLVSADWPEPSGISVDLVAAQRIND MQKLVTEVRRFRSDQGLADRQKVPARMHGVRDSDLSNQVAAVTSLAWLTEPGPDFEPSVSLEVRL GPEMNRTVWELDTSGTIDVAAERRRLEKELAGAQKELASTAAKLANADFLAKAPDAVIAKIRDRQRV AQQETERITTRLAALQ

>Rv2482c plsB2 TB.seq 2786915:2789281 MW:88284 SEQ ID NO:244 TKPAADASAVLTAEDTLVLASTATPVEMELIMGWLGQQRARHPDSKFDILKLPPRNAPPAALTALVE QLEPGFASSPQSGEDRSIVPVRVIWLPPADRSRAGKVAALLPGRDPYHPSQRQQRRILRTDPRRAR WAGESAKVSELRQQWRDTTVAEHKRDFAQFVSRRALLALARAEYRILGPQYKSPRLVKPEMLASA RFRAGLDRIPGATVEDAGKMLDELSTGWSQVSVDLVSVLGRLASRGFDPEFDYDEYQVAAMRAALE AHPAVLLFSHRSYIDGVWPVAMQDNRLPPVHMFGGINLSFGLMGPLMRRSGMIFIRRNIGNDPLYK YVLKEYVGYWEKRFNLSWSIEGTRSRTGKMLPPKLGLMSYVADAYLDGRSDDILLQGVSICFDQLH EITEYAAYARGAEKTPEGLRWLYNFIKAQGERNFGKIYVRFPEAVSMRQYLGAPHGELTQDPAAKRL ALQKMSFEVAWRILQATPVTATGLVSALLLTTRGTALTLDQLHHTLQDSLDYLERKQSPVSTSALRLR SREGVRAAADALSNGHPVTRVDSGREPVWYIAPDDEHAAAFYRNSVIHAFLETSIVELALAHAKHAE GDRVAAFWAQAMRLRDLLKFDFYFADSTAFRANIAQEMAWHQDWEDHLGVGGNEIDAMLYAKRPL MSDAMLRVFFEAYEIVADVLRDAPPDIGPEELTELALGLGRQFVAQGRVRSSEPVSTLLFATARQVAV DQELIAPAADLAERRVAFRRELRNILRDFDYVEQIARNQFVACEFKARQGRDRI

>Rv2509 - putative oxidoreductase TB.seq 2824676:2825479 MW:28014 SEQ ID NO:245

MPIPAPSPDARAWTGASQNIGAALATELAARGHHLIVTARREDVLTELAARLADKYRVTVDVRPADL ADPQERSKLADELAARPISILCANAGTATFGPIASLDLAGEKTQVQLNAVAVHDLTLAVLPGMIERKAG GILISGSAAGNSPIPYNATYAATKAFVNTFSESLRGELRGSGVHVTVLAPGPVRTELPDASEASLVEKL VPDFLWISTEHTARVSLNALERNKMRWPGLTSKAMSVASQYAPRAIVAPIVGAFYKRLGGS

>Rv2524c fas fatty acid synthase TB.seq 2840124:2849330 MW:326226 SEQ ID NO:246

VTIHEHDRVSADRGGDSPHTTHALVDRLMAGEPYAVAFGGQGSAWLETLEELVSATGIETELATLVG EAELLLDPVTDELIWRPIGFEPLQWVRALAAEDPVPSDKHLTSAAVSVPGVLLTQIAATRALARQGM DLVATPPVAMAGHSQGVLAVEALKAGGARDVELFALAQLIGAAGTLVARRRGISVLGDRPPMVSVTN ADPERIGRLLDEFAQDVRTVLPPVLSIRNGRRAWITGTPEQLSRFELYCRQISEKEEADRKNKVRGG DVFSPVFEPVQVEVGFHTPRLSDGIDIVAGWAEKAGLDVALARELADAILIRKVDWVDEITRVHAAGA RWILDLGPGDILTRLTAPVIRGLGIGIVPAATRGGQRNLFTVGATPEVARAWSSYAPTWRLPDGRVK LSTKFTRLTGRSPILLAGMTPTTVDAKIVAAAANAGHWAELAGGGQVTEEIFGNRIEQMAGLLEPGRT YQFNALFLDPYLWKLQVGGKRLVQKARQSGAAIDGWISAGIPDLDEAVELIDELGDIGISHWFKPGT IEQIRSVIRIATEVPTKPVIMHVEGGRAGGHHSWEDLDDLLLATYSELRSRANITVCVGGGIGTPRRAA EYLSGRWAQAYGFPLMPIDGILVGTAAMATKESTTSPSVKRMLVDTQGTDQWISAGKAQGGMASSR SQLGADIHEIDNSASRCGRLLDEVAGDAEAVAERRDEIIAAMAKTAKPYFGDVADMTYLQWLRRYVE LAIGEGNSTADTASVGSPWLADTWRDRFEQMLQRAEARLHPQDFGPIQTLFTDAGLLDNPQQAIAAL LARYPDAETVQLHPADVPFFVTLCKTLGKPVNFVPVIDQDVRRWWRSDSLWQAHDARYDADAVCIIP GTASVAGITRMDEPVGELLDRFEQAAIDEVLGAGVEPKDVASRRLGRADVAGPLAWLDAPDVRWA GRTVTNPVHRIADPAEWQVHDGPENPRATHSSTGARLQTHGDDVALSVPVSGTWVDIRFTLPANTV DGGTPVIATEDATSAMRTVLAIAAGVDSPEFLPAVANGTATLTVDWHPERVADHTGVTATFGEPLAP SLTNVPDALVGPCWPAVFAAIGSAVTDTGEPWEGLLSLVHLDHAARWGQLPTVPAQLTVTATAAN ATDTDMGRWPVSVWTGADGAVIATLEERFAILGRTGSAELADPARAGGAVSANATDTPRRRRRDV TITAPVDMRPFAWSGDHNPIHTDRAAALLAGLESPIVHGMWLSAAAQHAVTATDGQARPPARLVG WTARFLGMVRPGDEVDFRVERVGIDQGAEIVDVAARVGSDLVMSASARLAAPKTVYAFPGQGIQHK GMGMEVRARSKAARKVWDTADKFTRDTLGFSVLHNΛ/RDNPTSIIASGVHYHHPDGVLYLTQFTQVA MATVAAAQVAEMREQGAFVEGAIACGHSVGEYTALACVTGIYQLEALLEMVFHRGSKMHDIVPRDEL GRSNYRLAAIRPSQIDLDDADVPAFVAGIAESTGEFLEIVNFNLRGSQYAIAGTVRGLEALEAEVERRR ELTGGRRSFILVPGIDVPFHSRVLRVGVAEFRRSLDRVMPRDADPDLIIGRYIPNLVPRLFTLDRDFIQ EIRDLVPAEPLDEILADYDTWLRERPREMARTVFIELLAWQFASPVRWIETQDLLFIEEAAGGLGVERF VEIGVKSSPTVAGLATNTLKLPEYAHSTVEVLNAERDAAVLFATDTDPEPEPEEDEPVAESPAPDWS EAAPVAPAASSAGPRPDDLVFDAADATLALIALSAKMRIDQIEELDSIESITDGASSRRNQLLVDLGSE LNLGAIDGAAESDLAGLRSQVTKLARTYKPYGPVLSDAINDQLRTVLGPSGKRPGAIAERVKKTWELG EGWAKHVTVEVALGTREGSSVRGGAMGHLHEGALADAASVDKVIDAAVASVAARQGVSVALPSAG SGGGATIDAAALSEFTDQITGREGVLASAARLVLGQLGLDDPVNALPAAPDSELIDLVTAELGADWPR LVAPVFDPKKAWFDDRWASAREDLVKLWLTDEGDIDADWPRLAERFEGAGHWATQATWWQGKS LAAGRQIHASLYGRIAAGAENPEPGRYGGEVAWTGASKGSIAASWARLLDGGATVIATTSKLDEER LAFYRTLYRDHARYGAALWLVAANMASYSDVDALVEWIGTEQTESLGPQSIHIKDAQTPTLLFPFAAP RWGDLSEAGSRAEMEMKVLLWAVQRLIGGLSTIGAERDIASRLHWLPGSPNRGMFGGDGAYGEA KSALDAWSRWHAESSWAARVSLAHALIGWTRGTGLMGHNDAIVAAVEEAGVTTYSTDEMAALLLD LCDAESKVAAARSPIKADLTGGLAEANLDMAELAAKAREQMSAAAAVDEDAEAPGAIAALPSPPRGF TPAPPPQWDDLDVDPADLWIVGGAEIGPYGSSRTRFEMEVENELSAAGVLELAWTTGLIRWEDDP QPGWYDTESGEMVDESELVQRYHDAWQRVGIREFVDDGAIDPDHASPLLVSVFLEKDFAFWSSE ADARAFVEFDPEHTVIRPVPDSTDWQVIRKAGTEIRVPRKTKLSRWGGQIPTGFDPTVWGISADMA GSIDRLAVWNMVATVDAFLSSGFSPAEVMRYVHPSLVANTQGTGMGGGTSMQTMYHGNLLGRNKP NDIFQEVLPNIIAAHWQS GSYGAMIHPVAACATAAVSVEEGVDKIRLGKAQLWAGGLDDLTLEGII GFGDMAATADTSMMCGRGIHDSKFSRPNDRRRLGFVEAQGGGTILLARGDLALRMGLPVLAWAFA QSFGDGVHTSIPAPGLGALGAGRGGKDSPLARALAKLGVAADDVAVISKHDTSTLANDPNETELHER LADALGRSEGAPLFWSQKSLTGHAKGGAAVFQMMGLCQILRDGVIPPNRSLDCVDDELAGSAHFV WVRDTLRLGGKFPLKAGMLTSLGFGHVSGLVALVHPQAFIASLDPAQRADYQRRADARLLAGQRRL ASAIAGGAPMYQRPGDRRFDHHAPERPQEASMLLNPAARLGDGEAYIG

>Rv2555c alaS alanyl-tRNA synthase TB.seq 2873772:2876483 MW:97326 SEQ ID NO:247

VQTHEIRKRFLDHFVKAGHTEVPSASVILDDPNLLFVNAGMVQFVPFFLGQRTPPYPTATSIQKCIRTP

DIDEVGITTRHNTFFQMAGNFSFGDYFKRGAIELAWALLTNSLAAGGYGLDPERIWTTVYFDDDEAV

RLWQEVAGLPAERIQRRGMADNYWSMGIPGPCGPSSEIYYDRGPEFGPAGGPIVSEDRYLEVWNL VFMQNERGEGTTKEDYQILGPLPRKNIDTGMGVERIALVLQDVHNVYETDLLRPVIDTVARVAARAYD VGNHEDDVRYRIIADHSRTAAILIGDGVSPGNDGRGYVLRRLLRRVIRSAKLLGIDAAIVGDLMATVRN AMGPSYPELVADFERISRIAVAEETAFNRTLASGSRLFEEVASSTKKSGATVLSGSDAFTLHDTYGFPI ELTLEMAAETGLQVDEIGFRELMAEQRRRAKADAAARKHAHADLSAYRELVDAGATEFTGFDELRS QARILGIFVDGKRVPWAHGVAGGAGEGQRVELVLDRTPLYAESGGQIADEGTISGTGSSEAARAAV TDVQKIAKTLWVHRVNVESGEFVEGDTVIAAVDPGWRRGATQGHSGTHMVHAALRQVLGPNAVQA GSLNRPGYLRFDFNWQGPLTDDQRTQVEEVTNEAVQADFEVRTFTEQLDKAKAMGAIALFGESYPD EVRWEMGGPFSLELCGGTHVSNTAQIGPVTILGESSIGSGVRRVEAYVGLDSFRHLAKERALMAGL ASSLKVPSEEVPARVANLVERLRAAEKELERVRMASARAAATNAAAGAQRIGNVRLVAQRMSGGMT AADLRSLIGDIRGKLGSEPAWALIAEGESQTVPYAVAANPAAQDLGIRANDLVKQLAVAVEGRGGGK ADLAQGSGKNPTGIDAALDAVRSEIAVIARVG

>Rv2580c hisS histidyl-tRNA synthase TB.seq 2904822:2906090 MW:45118 SEQ ID NO:248

VTEFSSFSAPKGVPDYVPPDSAQFVAVRDGLLAAARQAGYSHIELPIFEDTALFARGVGESTDWSKE MYTFADRGDRSVTLRPEGTAGWRAVIEHGLDRGALPVKLCYAGPFFRYERPQAGRYRQLQQVGV EAIGVDDPALDAEVIAIADAGFRSLGLDGFRLEITSLGDESCRPQYRELLQEFLFGLDLDEDTRRRAGI NPLRVLDDKRPELRAMTASAPVLLDHLSDVAKQHFDTVLAHLDALGVPYVINPRMVRGLDYYTKTAF EFVHDGLGAQSGIGGGGRYDGLMHQLGGQDLSGIGFGLGVDRTVLALRAEGKTAGDSARCDVFGV PLGEAAKLRLAVLAGRLRAAGVRVDLAYGDRGLKGAMRAAARSGARVALVAGDRDIEAGTVAVKDL TTGEQVSVSMDSWAEVISRLAG

>Rv2614c thrS threonyl-tRNA synthase TB.seq 2941190:2943265 MW:77123 SEQ ID NO:249

MSAPAQPAPGVDGGDPSQARIRVPAGTTAATAVGEAGLPRRGTPDAIWVRDADGNLRDLSWVPD VDTDITPVAANTDDGRSVIRHSTAHVLAQAV^QELFPQAKLGIGPPITDGFYYDFDVPEPFTPEDLAALE KRMRQIVKEGQLFDRRVYESTEQARAELANEPYKLELVDDKSGDAEIMEVGGDELTAYDNLNPRTR ERVWGDLCRGPHIPTTKHIPAFKLTRSSAAYWRGDQKNASLQRIYGTAWESQEALDRHLEFIEEAQR RDHRKLGVELDLFSFPDEIGSGLAVFHPKGGIVRRELEDYSRRKHTEAGYQFVNSPHITKAQLFHTSG HLDWYADGMFPPMHIDAEYNADGSLRKPGQDYYLKPMNCPMHCLIFRARGRSYRELPLRLFEFGTV YRYEKSGWHGLTRVRGLTMDDAHIFCTRDQMRDELRSLLRFVLDLLADYGLTDFYLELSTKDPEKF VGAEEVWEEATTVLAEVGAESGLELVPDPGGAAFYGPKISVQVKDALGRTWQMSTIQLDFNFPERF GLEYTAADGTRHRPVMIHRALFGSIERFFGILTEHYAGAFPAWLAPVQWGIPVADEHVAYLEEVATQ LKSHGVRAEVDASDDRMAKKIVHHTNHKVPFMVLAGDRDVAAGAVSFRFGDRTQINGVARDDAVAA IVAWIADRENAVPTAELVKVAGRE

>Rv2697c dut deoxyuridine triphosphatase TB.seq 3013683:3014144 MW:15772 SEQ ID NO:250

VSTTLAIVRLDPGLPLPSRAHDGDAGVDLYSAEDVELAPGRRALVRTGVAVAVPFGMVGLVHPRSGL ATRVGLSIVNSPGTIDAGYRGEIKVALINLDPAAPIWHRGDRIAQLLVQRVELVELVEVSSFDEAGLAS TSRGDGGHGSSGGHASL

>Rv2782c pepR protease/peptidase, M16 family (insulinase) TB.seq 3089045:3090358 MW:47074 SEQ ID NO:251

MPRRSPADPAAALAPRRTTLPGGLRWTEFLPAVHSASVGVWVGVGSRDEGATVAGAAHFLEHLLF KSTPTRSAVDIAQAMDAVGGELNAFTAKEHTCYYAHVLGSDLPLAVDLVADWLNGRCAADDVEVER DWLEEIAMRDDDPEDALADMFLAALFGDHPVGRPVIGSAQSVSVMTRAQLQSFHLRRYTPERMW AAAGNVDHDGLVALVREHFGSRLVRGRRPVAPRKGTGRVNGSPRLTLVSRDAEQTHVSLGIRTPGR GWEHRWALSVLHTALGGGLSSRLFQEVRETRGLAYSVYSALDLFADSGALSVYAACLPERFADVMR VTADVLESVARDGITEAECGIAKGSLRGGLVLGLEDSSSRMSRLGRSELNYGKHRSIEHTLRQIEQVT VEEVNAVARHLLSRRYGAAVLGPHGSKRSLPQQLRAMVG

>Rv2783c gpsl pppGpp synthase and polyribonucleotide phosphorylase TB.seq

3090339:3092594 MW:79736 SEQ ID NO:252

MSAAEIDEGVFETTATIDNGSFGTRTIRFETGRLALQAAGAWAYLDDDNMLLSATTASKNPKEHFDF

FPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEIQIWTILSLDPGDLY DVLAINAASASTQLGGLPFSGPIGGVRVALIDGTWVGFPTVDQIERAVFDMWAGRIVEGDVAIMMVE AEATENWELVEGGAQAPTESWAAGLEAAKPFIAALCTAQQELADAAGKSGKPTVDFPVFPDYGED VYYSVSSVATDELAAALTIGGKAERDQRIDEIKTQWQRLADTYEGREKEVGAALRALTKKLVRQRILT DHFRIDGRGITDIRALSAEVAWPRAHGSALFERGETQILGVTTLDMIKMAQQIDSLGPETSKRYMHH YNFPPFSTGETGRVGSPKRREIGHGALAERALVPVLPSVEEFPYAIRQVSEALGSNGSTSMGSVCAS TLALLNAGVPLKAPVAGIAMGLVSDDIQVEGAVDGWERRFVTLTDILGAEDAFGDMDFKVAGTKDFV TALQLDTKLDGIPSQVLAGALEQAKDARLTILEVMAEAIDRPDEMSPYAPRVTTIKVPVDKIGEVIGPK GKVINAITEETGAQISIEDDGTVFVGATDGPSAQAAIDKINAIANPQLPTVGERFLGTWKTTDFGAFVS LPGRDGLVHISKLGKGKRIAKVEDWNVGDKLRVEIADIDKRGKISLILVADEDSTAAATDAATVTS

>Rv2793c truB tRNA pseudouridine 55 synthase TB.seq 3102364:3103257 MW:31821 SEQ ID NO:253

MSATGPGIWIDKPAGMTSHDWGRCRRIFATRRVGHAGTLDPMATGVLVIGIERATKILGLLTAAPKS YAATIRLGQTTSTEDAEGQVLQSVPAKHLTIEAIDAAMERLRGEIRQVPSSVSAIKVGGRRAYRLARQ GRSVQLEARPIRIDRFELLAARRRDQLIDIDVEIDCSSGTYIRALARDLGDALGVGGHVTALRRTRVGR FELDQARSLDDLAERPALSLSLDEACLLMFARRDLTAAEASAAANGRSLPAVGIDGVYAACDADGRVI ALLRDEGSRTRSVAVLRPATMHPG

>Rv2797c - TB.seq 3105619:3107304 MW:58761 SEQ ID NO:254

VPLTVADIDRWNAQAVREVFHAASARAEVTFEASRQLAALSIFANSGGKTAEAAAHHNAGIRRDLDA HGNEALAVARAADRAADGIVKVQSELAALRHAAAAAELTIDALINRWPIPGLRSTEAQWARTLAKQT ELQAELDAIMAEANAVDEELASAVNMADGDAPIPADSGPPVGPEGLTPTQLASDANEERLREERARL QAHLERLQAEYDQLSVRAARDYHNGILDGDAVGRLAALTDELSAARGRLGELDAVDEALSRAPETYL TQLQIPEDPNQQVLAAVAVGNPDTAANVSVTVPGVGSTTRGALPGMVTEARDLRSEVIRQLNAAGK PASVATIAWMGYHPPPNPLDTGSAGDLWQTMTDGQAHAGAADLSRYLQQVRANNPSGHLTVLGHS YGSLTASLALQDLDAQSAHPVNDWFYGSPGLELYSPAQLGLDHGHAYVMQAPHDLITNLVAPLAPL HGWGLDPYLTPGFTELSSQAGFDPGGIWRDGVYAHGDYPRSFLDAAGQPQLRMSGYNLAAIAAGL PDNTVGPPLLPPILGGGMPAAPGPALRGGR

>Rv2864c ponA2 TB.seq 3175454:3177262 MW:63015 SEQ ID NO:255

MVTKTTLASATSGLLLLAWAMSGCTPRPQGPGPAAEKFFAALAIGDTASAAQLSDNPNEAREALNA AWAGLQAAHLDAQVLSAKYAEDTGTVAYRFSWHLPKDRIWTYDGQLKMARDEGRWHVRWTTSGL HPKLGEHQTFALRADPPRRASVNEVGGTDVLVPGYLYHYSLDAGQAGRELFGTAHAWGALHPFDD TLNDPQLLAEQASSSTQPLDLVTLHADDSNRVAAAIGQLPGWITPQAELLPTDKHFAPAVLNDVKKA WDELDGKAGWRNΛ/SVNQNGVDVSVLHEVAPSPASSVSITLDRWQNAAQHAVNTRGGKAMIWIK PSTGEILAIAQNAGADADGPVATTGLYPPGSTFKMITAGAAVERDLATPETLLGCPGEIDIGHRTIPNY GGFDLGWPMSRAFASSCNTTFAELSSRLPPRGLTQAARRYGIGLDYQVDGITTVTGSVPPTVDLAE RTEDGFGQGKVLASPFGMALVAATVAAGKTPVPQLIAGRPTAVEGDATPISQKMIDALRPMMRLWT NGTAKEIAGCGEVFGKTGEAEFPGGSHSWFAGYRGDLAFASLIVGGGSSEYAVRMTKVMFESLPPG YLA

>Rv2868c gcpE TB.seq 3179368:3180528 MW:40451 SEQ ID NO:256

VTVGLGMPQPPAPTLAPRRATRQLMVGNVGVGSDHPVSVQSMCTTKTHDVNSTLQQIAELTAAGC DIVRVACPRQEDADALAEIARHSQIPWADIHFQPRYIFAAIDAGCAAVRVNPGNIKEFDGRVGEVAKA AGAAGIPIRIGVNAGSLDKRFMEKYGKATPEALVESALWEASLFEEHGFGDIKISVKHNDPWMVAAY ELLAARCDYPLHLGVTEAGPAFQGTIKSAVAFGALLSRGIGDTIRVSLSAPPVEEVKVGNQVLESLNL RPRSLEIVSCPSCGRAQVDVYTLANEVTAGLDGLDVPLRVAVMGCWNGPGEAREADLGVASGNGK GQIFVRGEVIKTVPEAQIVETLIEEAMRLAAEMGEQDPGATPSGSPIVTVS

>Rv2869c - TB.seq 3180548:3181759 MW:42835 SEQ ID NO:257 MMFVTGIVLFALAILISVALHECGHMWVARRTGMKVRRYFVGFGPTLWSTRRGETEYGVKAVPLGG FCDIAGMTPVEELDPDERDRAMYKQATWKRVAVLFAGPGMNLAICLVLIYAIALVWGLPNLHPPTRAV IGETGCVAQEVSQGKLEQCTGPGPAALAGIRSGDVWKVGDTPVSSFDEMAAAVRKSHGSVPIWE RDGTAIVTYVDIESTQRWIPNGQGGELQPATVGAIGVGAARVGPVRYGVFSAMPATFAVTGDLTVEV GKALAALPTKVGALVRAIGGGQRDPQTPISWGASIIGGDTVDHGLWVAFWFFLAQLNLILAAINLLPL LPFDGGHIAVAVFERIRNMVRSARGKVAAAPVNYLKLLPATYWLVLWGYMLLTVTADLVNPIRLFQ

>Rv2870c - TB.seq 3181770:3183077 MW:45324 SEQ ID NO:258 VATGGRWIRRRGDNEWAHNDEVTNSTDGRADGRLRVWLGSTGSIGTQALQVIADNPDRFEWG LAAGGAHLDTLLRQRAQTGVTNIAVADEHAAQRVGDIPYHGSDAATRLVEQTEADWLNALVGALGL RPTLAALKTGARLALANKESLVAGGSLVLRAARPGQIVPVDSEHSALAQCLRGGTPDEVAKLVLTAS GGPFRGWSAADLEHVTPEQAGAHPTWSMGPMNTLNSASLVNKGLEVIETHLLFGIPYDRIDWVHP QSIIHSMVTFIDGSTIAQASPPDMKLPISLALGWPRRVSGAAAACDFHTASSWEFEPLDTDVFPAVEL ARQAGVAGGCMTAVYNAANEEAAAAFLAGRIGFPAIVGIIADVLHAADQWAVEPATVDDVLDAQRWA RERAQRAVSGMASVAIASTAKPGAAGRHASTLERS

>Rv2922c smc member of Smc1/Cut3/Cut14 family TB.seq 3234189:3238055 MW:139610 SEQ ID NO:259

VGAGSRFPLVDPLPSVGARPDRLRGQPRRRTRAGGRPGSARCVPEAAAAAAGRHDTGPRRQSRR RLVAVDGADHRVQRAVIWPLVYLKSLTLKGFKSFAAPTTLRFEPGITANΛ/GPNGSGKSNWDALAWV MGEQGAKTLRGGKMEDVIFAGTSSRAPLGRAEVTVSIDNSDNALPIEYTEVSITRRMFRDGASEYEIN GSSCRLMDVQELLSDSGIGREMHVIVGQGKLEEILQSRPEDRRAFIEEAAGVLKHRKRKEKALRKLDT MAANLARLTDLTTELRRQLKPLGRQAEAAQRAAAIQADLRDARLRLAADDLVSRRAEREAVFQAEAA MRREHDEAAARLAVASEELAAHESAVAELSTRAESIQHTWFGLSALAERVDATVRIASERAHHLDIEP VAVSDTDPRKPEELEAEAQQVAVAEQQLLAELDAARARLDAARAELADRERRAAEADRAHLAAVRE EADRREGLARLAGQVETMRARVESIDESVARLSERIEDAAMRAQQTRAEFETVQGRIGELDQGEVG LDEHHERTVAALRLADERVAELQSAERAAERQVASLRARIDALAVGLQRKDGAAWLAHNRSGAGLF GSIAQLVKVRSGYEAALAAALGPAADALAVDGLTAAGSAVSALKQADGGRAVLVLSDWPAPQAPQS ASGEMLPSGAQWALDLVESPPQLVGAMIAMLSGVAWNDLTEAMGLVEIRPELRAVTVDGDLVGAG WVSGGSDRKLSTLEVTSEIDKARSELAAAEALAAQLNAALAGALTEQSARQDAAEQALAALNESDTAI SAMYEQLGRLGQEARAAEEEWNRLLQQRTEQEAVRTQTLDDVIQLETQLRKAQETQRVQVAQPIDR QAISAAADRARGVEVEARLAVRTAEERANAVRGRADSLRRAAAAEREARVRAQQARAARLHAAAVA AAVADCGRLLAGRLHRAVDGASQLRDASAAQRQQRLAAMAAVRDEVNTLSARVGELTDSLHRDEL ANAQAALRIEQLEQMVLEQFGMAPADLITEYGPHVALPPTELEMAEFEQARERGEQVIAPAPMPFDR VTQERRAKRAERALAELGRVNPLALEEFAALEERYNFLSTQLEDVKAARKDLLGWADVDARILQVFN DAFVDVEREFRGVFTALFPGGEGRLRLTEPDDMLTTGIEVEARPPGKKITRLSLLSGGEKALTAVAML VAIFRARPSPFYIMDEVEAALDDVNLRRLLSLFEQLREQSQIIIITHQKPTMEVADALYGVTMQNDGITA VISQRMRGQQVDQLVTNSS

>Rv2925c rnc RNAse III TB.seq 3239829:3240548 MW:25400 SEQ ID NO:260 MIRSRQPLLDALGVDLPDELLSLALTHRSYAYENGGLPTNERLEFLGDAVLGLTITDALFHRHPDRSE GDLAKLRASWNTQALADVARRLCAEGLGVHVLLGRGEANTGGADKSSILADGMESLLGAIYLQHGM EKAREVILRLFGPLLDAAPTLGAGLDWKTSLQELTAARGLGAPSYLVTSTGPDHDKEFTAWWMDS EYGSGVGRSKKEAEQKAAAAAWKALEVLDNAMPGKTSA

>Rv2934 ppsD TB.seq 3262245:3267725 MW: 193317 SEQ ID NO:261 MTSLAERAAQLSPNARAALARELVRAGTTFPTDICEPVAWGIGCRFPGNVTGPESFWQLLADGVDT IEQVPPDRWDADAFYDPDPSASGRMTTKWGGFVSDVDAFDADFFGITPREAVAMDPQHRMLLEVA WEALEHAGIPPDSLSGTRTGVMMGLSSWDYTIVNIERRADIDAYLSTGTPHCAAVGRIAYLLGLRGPA VAVDTACSSSLVAIHLACQSLRLRETDVALAGGVQLTLSPFTAIALSKWSALSPTGRCNSFDANADGF VRGEGCGVWLKRLADAVRDQDRVLAWRGSATNSDGRSNGMTAPNALAQRDVITSALKLADVTPD SVNYVETHGTGTVLGDPIEFESLAATYGLGKGQGESPCALGSVKTNIGHLEAAAGVAGFIKAVLAVQR GHIPRNLHFTRWNPAIDASATRLFVPTESAPWPAAAGPRRAAVSSFGLSGTNAHVWEQAPDTAVAA AGGMPYVSALNVSGKTAARVASAAAVLADWMSGPGAAAPLADVAHTLNRHRARHAKFATVIARDRA EAIAGLRALAAGQPRVGWDCDQHAGGPGRVFVYSGQGSQWASMGQQLLANEPAFAKAVAELDPI FVDQVGFSLQQTLIDGDEWGIDRIQPVLVGMQLALTELWRSYGVIPDAVIGHSMGEVSAAWAGALT PEQGLRVITTRSRLMARLSGQGAMALLELDADAAEALIAGYPQVTLAVHASPRQTVIAGPPEQVDTVI AAVATQNRLARRVEVDVASHHPIIDPILPELRSALADLTPQPPSIPIISTTYESAQPVADADYWSANLRN PVRFHQAVTAAGVDHNTFIEISPHPVLTHALTDTLDPDGSHTVMSTMNRELDQTLYFHAQLAAVGVA ASEHTTGRLVDLPPTPWHHQRFWVTDRSAMSELAATHPLLGAHIEMPRNGDHVWQTDVGTEVCPW LADHKVFGQPIMPAAGFAEIALAAASEALGTAADAVAPNIVINQFEVEQMLPLDGHTPLTTQLIRGGDS QIRVEIYSRTRGGEFCRHATAKVEQSPRECAHAHPEAQGPATGTTVSPADFYALLRQTGQHHGPAF AALSRIVRLADGSAETEISIPDEAPRHPGYRLHPWLDAALQSVGAAIPDGEIAGSAEASYLPVSFETIR VYRDIGRHVRCRAHLTNLDGGTGKMGRIVLINDAGHIAAEVDGIYLRRVERRAVPLPLEQKIFDAEWT ESPIAAVPAPEPAAETTRGSWLVLADATVDAPGKAQAKSMADDFVQQWRSPMRRVHTADIHDESAV LAAFAETAGDPEHPPVGNΛΛ/FVGGASSRLDDELAAARDTVWSITTVVRAVVGTWHGRSPRLWLVTG GGLSVADDEPGTPAAASLKGLVRVLAFEHPDMRTTLVDLDITQDPLTALSAELRNAGSGSRHDDVIA WRGERRFVERLSRATIDVSKGHPWRQGASYWTGGLGGLGLWARWLVDRGAGRWLGGRSDPT DEQCNVLAELQTRAEIWVRGDVASPGVAEKLIETARQSGGQLRGWHAAAVIEDSLVFSMSRDNLE RVWAPKATGALRMHEATADCELDWWLGFSSAASLLGSPGQAAYACASAWLDALVGWRRASGLPA AVINWGPWSEVGVAQALVGSVLDTISVAEGIEALDSLLAADRIRTGVARLRADRALVAFPEIRSISYFT QWEELDSAGDLGDWGGPDALADLDPGEARRAVTERMCARIAAVMGYTDQSTVEPAVPLDKPLTEL GLDSLMAVRIRNGARADFGVEPPVALILQGASLHDLTADLMRQLGLNDPDPALNNADTIRDRARQRA AARHGAAMRRRPKPEVQGG

>Rv2946c pksl TB.seq 3291503:3296350 MW: 166642 SEQ ID NO:262 VISARSAEALTAQAGRLMAHVQANPGLDPIDVGCSLASRSVFEHRAVWGASREQLIAGLAGLAAGE PGAGVAVGQPGSVGKTVWFPGQGAQRIGMGRELYGELPVFAQAFDAVADELDRHLRLPLRDVIW GADADLLDSTEFAQPALFAVEVASFAVLRDWGVLPDFVMGHSVGELAAAHAAGVLTLADAAMLWA RGRLMQALPAGGAMVAVAASEDEVEPLLGEGVGIAAINAPESWISGAQAAANAIADRFAAQGRRVH QLAVSHAFHSPLMEPMLEEFARVAARVQAREPQLGLVSNVTGELAGPDFGSAQYWVDHVRRPVRF ADSARHLQTLGATHFIEAGPGSGLTGSIEQSLAPAEAMWSMLGKDRPELASALGAAGQVFTTGVPV QWSAVFAGSGGRRVQLPTYAFQRRRFWETPGADGPADAAGLGLGATEHALLGAWERPDSDEWL TGRLSLADQPWLADHWNGWLFPGAGFVELVIRAGDEVGCALIEELVLAAPLVMHPGVGVQVQVW GAADESGHRAVSVYSRGDQSQGWLLNAEGMLGVAAAETPMDLSVWPPEGAESVDISDGYAQLAE RGYAYGPAFQGLVAIWRRGSELFAEWAPGEAGVAVDRMGMHPAVLDAVLHALGLAVEKTQASTET RLPFCWRGVSLHAGGAGRVRARFASAGADAISVDVCDATGLPVLTVRSLVTRPITAEQLRAAVTAAG GASDQGPLEWWSPISWSGGANGSAPPAPVSWADFCAGSDGDASVWWELESAGGQASSWGS VYAATHTALEVLQSWLGADRAATLWLTHGGVGLAGEDISDLAAAAVWGMARSAQAENPGRIVLIDT DAAVDASVLAGVGEPQLLVRGGTVHAPRLSPAPALLALPAAESAWRLAAGGGGTLEDLVIQPCPEV QAPLQAGQVRVAVAAVGVNFRDWAALGMYPGQAPPLGAEGAGWLETGPEVTDLAVGDAVMGFL GGAGPLAWDQQLVTRVPQGWSFAQAAAVPWFLTAWYGLADLAEIKAGESVLIHAGTGGVGMAAV QLARQWGVEVFVTASRGKWDTLRAMGFDDDHIGDSRTCEFEEKFLAVTEGRGVDWLDSLAGEFV DASLRLLVRGGRFLEMGKTDIRDAQEIAANYPGVQYRAFDLSEAGPARMQEMLAEVRELFDTRELH RLPVTTWDVRCAPAAFRFMSQARHIGKWLTMPSALADRLADGTWITGATGAVGGVLARHLVGAY GVRHLVLASRRGDRAEGAAELAADLTEAGAKVQWACDVADRAAVAGLFAQLSREYPPVRGVIHAA GVLDDAVITSLTPDRIDTVLRAKVDAAWNLHQATSDLDLSMFALCSSIAATVGSPGQGNYSAANAFLD GLAAHRQAAGLAGISLAWGLWEQPGGMTAHLSSRDLARMSRSGLAPMSPAEAVELFDAALAIDHPL AVATLLDRAALDARAQAGALPALFSGLARRPRRRQIDDTGDATSSKSALAQRLHGLAADEQLELLVG LVCLQAAAVLGRPSAEDVDPDTEFGDLGFDSLTAVELRNRLKTATGLTLPPTVIFDHPTPTAVAEYVA QQMSGSRPTESGDPTSQWEPAAAEVSVHA

>Rv3014c ligA DNA ligase TB.seq 3372545:3374617 MW:75258 SEQ ID NO:263

VSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPELRTPDSP TQLVGGAGFATDFEPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLCELKIDGVALSLVYR EGRLTRASTRGDGRTGEDVTLNARTIADVPERLTPGDDYPVPEVLEVRGEVFFRLDDFQALNASLVE EGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGHVEGFRPATLHQAYLALRAWGLPVSEHT TLATDLAGVRERIDYWGEHRHEVDHEIDGNΛΛ/KVDEVALQRRLGSTSRAPRWAIAYKYPPEEAQTKL LDIRVNVGRTGRITPFAFMTPVKVAGSTVGQATLHNASEIKRKGVLIGDTWIRKAGDVIPEVLGPWE LRDGSEREFIMPTTCPECGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAG VALLQAKVIADEGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGP TAARALATEFGSLDAIAAASTDQLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRMVDERD ESVPRTLAGLTIWTGSLTGFSRDDAKEAIVARGGKAAGSVSKKTNYWAGDSPGSKYDKAVELGVPI LDEDGFRRLLADGPASRT >Rv3025c - NifS-like protein TB.seq 3383885:3385063 MW:40948 SEQ ID NO:264

MAYLDHAATTPMHPAAIEAMAAVQRTIGNASSLHTSGRSARRRIEEARELIADKLGARPSEVIFTAGG TESDNLAVKGIYWARRDAEPHRRRIVTTEVEHHAVLDSVNWLVEHEGAHVTWLPTAADGSVSATAL REALQSHDDVALVSVMWANNEVGTILPIAEMSWAMEFGVPMHSDAIQAVGQLPLDFGASGLSAMS VAGHKFGGPPGVGALLLRRDVTCVPLMHGGGQERDIRSGTPDVASAVGMATAAQIAVDGLEENSAR LRLLRDRLVEGVLAEIDDVCLNGADDPMRLAGNAHFTFRGCEGDALLMLLDANGIECSTGSACTAGV AQPSHVLIAMGVDAASARGSLRLSLGHTSVEADVDAALEVLPGAVARARRAALAAAGASR

>Rv3080c pknK serine-threonine protein kinase TB.seq 3442656:3445985 MW:119420 SEQ ID NO:265

MTDVDPHATRRDLVPNIPAELLEAGFDNVEEIGRGGFGWYRCVQPSLDRAVAVKVLSTDLDRDNLE RFLREQRAMGRLSGHPHIVTVLQVGVLAGGRPFIVMPYHAKNSLETLIRRHGPLDWRETLSIGVKLA GALEAAHRVGTLHRDVKPGNILLTDYGEPQLTDFGIARIAGGFETATGVIAGSPAFTAPEVLEGASPTP ASDVYSLGATLFCALTGHAAYERRSGERVIAQFLRITSQPIPDLRKQGLPADVAAAIERAMARHPADR PATAADVGEELRDVQRRNGVSVDEMPLPVELGVERRRSPEAHAAHRHTGGGTPTVPTPPTPATKY RPSVPTGSLVTRSRLTDILRAGGRRRLILIHAPSGFGKSTLAAQWREELSRDGAAVAWLTIDNDDNNE VWFLSHLLESIRRVRPTLAESLGHVLEEHGDDAGRYVLTSLIDEIHENDDRIAWIDDWHRVSDSRTQ AALGFLLDNGCHHLQLIVTSWSRAGLPVGRLRIGDELAEIDSAALRFDTDEAAALLNDAGGLRLPRAD VQALTTSTDGWAAALRLAALSLRGGGDATQLLRGLSGASDVIHEFLSENVLDTLEPELREFLLVASVT ERTCGGLASALAGITNGRAMLEEAEHRGLFLQRTEDDPNWFRFHQMFADFLHRRLERGGSHRVAEL HRRASAWFAENGYLHEAVDHALAAGDPARAVDLVEQDETNLPEQSKMTTLLAIVQKLPTSMWSRA RLQLAIAWANILLQRPAPATGALNRFETALGRAELPEATQADLRAEADVLRAVAEVFADRVERVDDLL AEAMSRPDTLPPRVPGTAGNTAALAAICRFEFAEVYPLLDWAAPYQEMMGPFGTVYAQCLRGMAAR NRLDIVAALQNFRTAFEVGTAVGAHSHAARLAGSLLAELLYETGDLAGAGRLMDESYLLGSEGGAVD YLAARYVIGARVKAAQGDHEGAADRLSTGGDTAVQLGLPRLAARINNERIRLGIALPAAVAADLLAPR TIPRDNGIATMTAELDEDSAVRLLSAGDSADRDQACQRAGALAAAIDGTRRPLAALQAQILHIETLAAT GRESDARNELAPVATKCAELGLSRLLVDAGLA >Rv3106 fprA adrenodoxin and NADPH ferredoxin reductase TB.seq 3474004:3475371

MW:49342 SEQ ID NO:266

MRPYYIAIVGSGPSAFFAAASLLKAADTTEDLDMAVDMLEMLPTPWGLVRSGVAPDHPKIKSISKQFE KTAEDPRFRFFGNVWGEHVQPGELSERYDAVIYAVGAQSDRMLNIPGEDLPGSIAAVDFVGWYNA HPHFEQVSPDLSGARAWIGNGNVALDVARILLTDPDVLARTDIADHALESLRPRGIQEWIVGRRGPL QAAFTTLELRELADLDGVDWIDPAELDGITDEDAAAVGKVCKQNIKVLRGYADREPRPGHRRMVFR FLTSPIEIKGKRKVERIVLGRNELVSDGSGRVAAKDTGEREELPAQLWRSVGYRGVPTPGLPFDDQ SGTIPNVGGRINGSPNEYWGWIKRGPTGVIGTNKKDAQDTVDTLIKNLGNAKEGAECKSFPEDHAD QVADWLAARQPKLVTSAHWQVIDAFERAAGEPHGRPRVKLASLAELLRIGLG

>Rv3235 - TB.seq 3611296:3611934 MW:22659 SEQ ID NO:267 MMASNQTAAQHSSATLQQAPRSIDDAGGCPLTISPIANSPGDTFAVTPWEYEPPPRNIPPCGQSSH AARRPHTPQLARRQPIRPSGRAPAAVTSTAKSPRLRQAGTFADAALRRVLEVIDRRRPVGQLRPLLA PGLVDSVLAVSRTAAGHQQGAAMLRRIRLTPAGPDTADTAAEVFGTYSRGDRIHAIACRVEQRPAGN ETRWLMVALHIG

>Rv3255c manA mannose-6-phosphate isomerase TB.seq 3635040:3636263 MW:43340 SEQ ID NO:268 VELLRGALRTYAWGSRTAIAEFTGRPVPAAHPEAELWFGAHPGDPAWLQTPHGQTSLLEALVADPE GQLGSASRARFGDVLPFLVKVLAADEPLSLQAHPSAEQAVEGYLREERMGIPVSSPVRNYRDTSHK PELLVALQPFEALAGFREAARTTELLRALAVSDLDPFIDLLSEGSDADGLRALFTTWITAPQPDIDVLV PAVLDGAIQYVSSGATEFGAEAKTVLELGERYPGDAGVLAALLLNRISLAPGEAIFLPAGNLHAYVRG FGVEVMANSDNVLRGGLTPKHVDVPELLRVLDFAPTPKARLRPPIRREGLGLVFETPTDEFAATLLVL DGDHLGHEVDASSGHDGPQILLCTEGSATVHGKCGSLTLQRGTAAWVAADDGPIRLTAGQPAKLFR ATVGL

>Rv3264c rmlA2 glucose-1 -phosphate thymidyltransferase TB.seq 3644897:3645973 MW:37840 SEQ ID NO:269

LATHQVDAWLVGGKGTRLRPLTLSAPKPMLPTAGLPFLTHLLSRIAAAGIEHVILGTSYKPAVFEAEF GDGSALGLQIEYVTEEHPLGTGGGIANVAGKLRNDTAMVFNGDVLSGADLAQLLDFHRSNRADVTL QLVRVGDPRAFGCVPTDEEDRWAFLEKTEDPPTDQINAGCYVFERNVIDRIPQGREVSVEREVFPA LLADGDCKIYGYVDASYWRDMGTPEDFVRGSADLVRGIAPSPALRGHRGEQLVHDGAAVSPGALLI GGTWGRGAEIGPGTRLDGAVIFDGVRVEAGCVIERSIIGFGARIGPRALIRDGVIGDGADIGARCELL SGARVWPGVFLPDGGIRYSSDV

>Rv3368c - TB.seq 3780334:3780975 MW:23734 SEQ ID NO:270

MTLNLSVDEVLTTTRSVRKRLDFDKPVPRDVLMECLELALQAPTGSNSQGWQWVFVEDAAKKKAIA DVYLANARGYLSGPAPEYPDGDTRGERMGRVRDSATYLAEHMHRAPVLLIPCLKGREDESAVGGVS FWASLFPAVWSFCLALRSRGLGSCWTTLHLLDNGEHKVADVLGIPYDEYSQGGLLPIAYTQGIDFRP AKRLPAESVTHWNGW

>Rv3382c lytB1 TB.seq 3796447:3797433 MW:34667 SEQ ID NO:271

MAEVFVGPVAQGYASGEVTVLLASPRSFCAGVERAIETVKRVLDVAEGPVYVRKQIVHNTVWAELR DRGAVFVEDLDEIPDPPPPGAVWFSAHGVSPAVRAGADERGLQWDATCPLVAKVHAEAARFAAR GDTWFIGHAGHEETEGTLGVAPRSTLLVQTPADVAALNLPEGTQLSYLTQTTLALDETADVIDALRA RFPTLGQPPSEDICYATTNRQRALQSMVGECDWLVIGSCNSSNSRRLVELAQRSGTPAYLIDGPDDI EPEWLSSVSTIGVTAGASAPPRLVGQVIDALRGYASITWERSIATETVRFGLPKQVRAQ

>Rv3418c groES 10 kD chaperone TB.seq 3836985:3837284 MW:10773 SEQ ID NO:272 VAKVNIKPLEDKILVQANEAETTTASGLVIPDTAKEKPQEGTWAVGPGRWDEDGEKRIPLDVAEGDT VIYSKYGGTEIKYNGEEYLILSARDVLAWSK >Rv3423c air TB.seq 3840193:3841416 MW.43357 SEQ ID NO:273

VKRFWENVGKPNDTTDGRGTTSLAMTPISQTPGLLAEAMVDLGAIEHNVRVLREHAGHAQLMAWK ADGYGHGATRVAQTALGAGAAELGVATVDEALALRADGITAPVLAWLHPPGIDFGPALLADVQVAVS SLRQLDELLHAVRRTGRTATVTVKVDTGLNRNGVGPAQFPAMLTALRQAMAEDAVRLRGLMSHMV YADKPDDSINDVQAQRFTAFLAQAREQGVRFEVAHLSNSSATMARPDLTFDLVRPGIAVYGLSPVPA LGDMGLVPAMTVKCAVALVKSIRAGEGVSYGHTWIAPRDTNLALLPIGYADGVFRSLGGRLEVLINGR RCPGVGRICMDQFMVDLGPGPLDVAEGDEAILFGPGIRGEPTAQDWADLVGTIHYEWTSPRGRITR TYREAENR

>Rv3490 otsA [alpha],-trehalose-phosphate synthase TB.seq 3908232:3909731 MW:55864

SEQ ID NO:274

MAPSGGQEAQICDSETFGDSDFVWANRLPVDLERLPDGSTTWKRSPGGLVTALEPVLRRRRGAW VGWPGVNDDGAEPDLHVLDGPIIQDELELHPVRLSTTDIAQYΎEGFSNATLWPLYHDVIVKPLYHRE WWDRYVDVNQRFAEAASRAAAHGATVWVQDYQLQLVPKMLRMLRPDLTIGFFLHIPFPPVELFMQ MPWRTEIIQGLLGADLVGFHLPGGAQNFLILSRRLVGTDTSRGTVGVRSRFGAAVLGSRTIRVGAFPI SVDSGALDHAARDRNIRRRAREIRTELGNPRKILLGVDRLDYTKGIDVRLKAFSELLAEGRVKRDDTV WQLATPSRERVESYQTLRNDIERQVGHINGEYGEVGHPWHYLHRPAPRDELIAFFVASDVMLVTP LRDGMNLVAKEYVACRSDLGGALVLSEFTGAAAELRHAYLVNPHDLEGVKDGIEEALNQTEEAGRR MRSLRRQVLAHDVDRWAQSFLDALAGAHPRGQG

>Rv3598c lysS lysyl-tRNA synthase TB.seq 4041423:4042937 MW:55678 SEQ ID NO:275

VSAADTAEDLPEQFRIRRDKRARLLAQGRDPYPVAVPRTHTLAEVRAAHPDLPIDTATEDIVGVAGRV IFARNSGKLCFATLQDGDGTQLQVMISLDKVGQAALDAWKADVDLGDIVYVHGAVISSRRGELSVLA DCWRIAAKSLRPLPVAHKEMSEESRVRQRYVDLIVRPEARAVARLRIAWRAIRTALQRRGFLEVETP VLQTLAGGAAARPFATHSNALDIDLYLRIAPELFLKRCIVGGFDKVFELNRVFRNEGADSTHSPEFSM LETYQTYGTYDDSAWTRELIQEVADEAIGTRQLPLPDGSVYDIDGEWATIQMYPSLSVALGEEITPQT TVDRLRGIADSLGLEKDPAIHDNRGFGHGKLIEELWERTVGKSLSAPTFVKDFPVQTTPLTRQHRSIP GVTEKWDLYLRGIELATGYSELSDPWQRERFADQARAAAAGDDEAMVLDEDFLAALEYGMPPCTG TGMGIDRLLMSLTGLSIRETVLFPIVRPHSN

>Rv3600c - similar to Bacillus subtilis protein YacB TB.seq 4043041 :4043856 MW:29274

SEQ ID NO:276

VLLAIDVRNTHTWGLLSGMKEHAKWQQWRIRTESEVTADELALTIDGLIGEDSERLTGTAALSTVPS

VLHEVRIMLDQYWPSVPHVLIEPGVRTGIPLLVDNPKEVGADRIVNCLAAYDRFRKAAIWDFGSSICV DWSAKGEFLGGAIAPGVQVSSDAAAARSAALRRVELARPRSWGKNTVECMQAGAVFGFAGLVDG

LVGRIREDVSGFSVDHDVAIVATGHTAPLLLPELHTVDHYDQHLTLQGLRLVFERNLEVQRGRLKTAR >Rv3606c folK 7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase TB.seq

4048181 :4048744 MW:20732 SEQ ID NO:277

MTRWLSVGSNLGDRLARLRSVADGLGDALIAASPIYEADPWGGVEQGQFLNAVLIADDPTCEPREW LRRAQEFERAAGRVRGQRWGPRNLDVDLIACYQTSATEALVEVTARENHLTLPHPLAHLRAFVLIPW IAVDPTAQLTVAGCPRPVTRLLAELEPADRDSVRLFRPSFDLNSRHPVSRAPES

>Rv3607c folX may be involved in folate biosynthesis TB.seq 4048744:4049142 MW:14553

MADRIELRGLTVHGRHGVYDHERVAGQRFVIDVTVWIDLAEAANSDDLADTYDYVRLASRAAEIVAG PPRKLIETVGAEIADHVMDDQRVHAVEVAVHKPQAPIPQTFDDVAWIRRSRRGGRGWWPAGGAV

>Rv3608c folP dihydropteroate synthase TB.seq 4049138:4049977 MW:28812 SEQ ID NO:278

VSPAPVQVMGVLNVTDDSFSDGGCYLDLDDAVKHGLAMAAAGAGIVDVGGESSRPGATRVDPAVE TSRVIPWKELAAQGITVSIDTMRADVARAALQNGAQMVNDVSGGRADPAMGPLLAEADVPWVLMH WRAVSADTPHVPVRYGNWAEVRADLLASVADAVAAGVDPARLVLDPGLGFAKTAQHNWAILHALP ELVATGIPVLVGASRKRFLGALLAGPDGVMRPTDGRDTATAVISALAALHGAWGVRVHDVRASVDAI KWEAWMGAERIERDG

>Rv3609c folE GTP cyclohydrolase I TB.seq 4049977:4050582 MW:22395 SEQ ID NO:279

MSQLDSRSASARIRVFDQQRAEAAVRELLYAIGEDPDRDGLVATPSRVARSYREMFAGLYTDPDSVL

NTMFDEDHDELVLVKEIPMYSTCEHHLVAFHGVAHVGYIPGDDGRVTGLSKIARLVDLYAKRPQVQE

RLTSQIADALMKKLDPRGVIWIEAEHLCMAMRGVRKPGSVTTTSAVRGLFKTNAASRAEALDLILRK >Rv3610c ftsH inner membrane protein, chaperone TB.seq 4050601 :4052880 MW:81987

MNRKNVTRTITAIAVWLLGWSFFYFSDDTRGYKPVDTSVAITQINGDNVKSAQIDDREQQLRLILKKG NNETDGSEKVITKYPTGYAVDLFNALSAKNAKVSI A/NQGSILGELLVYVLPLLLLVGLFVMFSRMQG GARMGFGFGKSRAKQLSKDMPKTTFADVAGVDEAVEELYEIKDFLQNPSRYQALGAKIPKGVLLYGP PGTGKTLLARAVAGEAGVPFFTISGSDFVEMFVGVGASRVRDLFEQAKQNSPCIIFVDEIDAVGRQR GAGLGGGHDEREQTLNQLLVEMDGFGDRAGVILIAATNRPDILDPALLRPGRFDRQIPVSNPDLAGR RAVLRVHSKGKPMAADADLDGLAKRTVGMTGADLANVINEAALLTARENGTVITGPALEEAVDRVIG GPRRKGRIISEQEKKITAYHEGGHTLAAWAMPDIEPIYKVTILARGRTGGHAVAVPEEDKGLRTRSEMI AQLVFAMGGRAAEELVFREPTTGAVSDIEQATKIARSMVTEFGMSSKLGAVKYGSEHGDPFLGRTM GTQPDYSHEVAREIDEEVRKLIEAAHTEAWEILTEYRDVLDTLAGELLEKETLHRPELESIFADVEKRP RLTMFDDFGGRIPSDKPPIKTPGELAIERGEPWPQPVPEPAFKAAIAQATQAAEAARSDAGQTGHGA NGSPAGTHRSGDRQYGSTQPDYGAPAGWHAPGWPPRSSHRPSYSGEPAPTYPGQPYPTGQADP GSDESSAEQDDEVSRTKPAHG

>Rv3671c - TB.seq 4112322:4113512 MW.40722 SEQ ID NO:280

MTPSQWLDIAVLAVAFIAAISGWRAGALGSMLSFGGVLLGATAGVLLAPHIVSQISAPRAKLFAALFLIL ALWVGEVAGWLGRAVRGAIRNRPIRLIDSVIGVGVQLVWLTAAWLLAMPLTQSKEQPELAAAVKG

SRVLARVNEAAPTWLKTVPKRLSALLNTSGLPAVLEPFSRTPVIPVASPDPALVNNPWAATEPSWKI

RSLAPRCQKVLEGTGFVISPDRVMTNAHWAGSNNVTVYAGDKPFEATWSYDPSVDVAILAVPHLP PPPLVFAAEPAKTGADVWLGYPGGGNFTATPARIREAIRLSGPDIYGDPEPVTRDVYTIRADVEQGD SGGPLIDLNGQVLGWFGAAIDDAETGFVLTAGEVAGQLAKIGATQPVGTGACVS

>Rv3682 ponA2 TB.seq 4121913:4124342 MW:84637 SEQ ID NO:281

MPERLPAAITVLKLAGCCLLASWATALTFPFAGGLGLMSNRASEWANGSAQLLEGQVPAVSTMVD AKGNTIAWLYSQRRFEVPSDKIANTMKLAIVSIEDKRFADHSGVDWKGTLTGLAGYASGDLDTRGGS TLEQQYVKNYQLLVTAQTDAEKRAAVETTPARKLREIRMALTLDKTFTKSEILTRYLNLVSFGNNSFG VQDAAQTYFGINASDLNWQQAALLAGMVQSTSTLNPYTNPDGALARRNWLDTMIENLPGEAEALR AAKAEPLGVLPQPNELPRGCIAAGDRAFFCDYVQEYLSRAGISKEQVATGGYLIRTTLDPEVQAPVKA AIDKYASPNLAGISSVMSVIKPGKDAHKVLAMASNRKYGLDLEAGETMRPQPFSLVGDGAGSIFKIFT TAAALDMGMGINAQLDVPPRFQAKGLGSGGAKGCPKETWCWNAGNYRGSMNVTDALATSPNTAF AKLISQVGVGRAVDMAIKLGLRSYANPGTARDYNPDSNESLADFVKRQNLGSFTLGPIELNALELSNV AATLASGGVWCPPNPIDQLIDRNGNEVAVTTETCDQWPAGLANTLANAMSKDAVGSGTAAGSAGA AGWDLPMSGKTGTTEAHRSAGFVGFTNRYAAANYIYDDSSSPTDLCSGPLRHCGSGDLYGGNEPS RTWFAAMKPIANNFGEVQLPPTDPRYVDGAPGSRVPSVAGLDVDAARQRLKDAGFQVADQTNSVN SSAKYGEWGTSPSGQTIPGSIVTIQISNGIPPAPPPPPLPEDGGPPPPVGSQWEIPGLPPITIPLLAP

PPPAPPP

>Rv3721c dnaZX DNA polymerase III, [gamma] (dnaZ) and t (dnaX) TB.seq 4164995:4166728

MW:61892 SEQ ID NO:282 VALYRKYRPASFAEWGQEHVTAPLSVALDAGRINHAYLFSGPRGCGKTSSARILARSLNCAQGPTA NPCGVCESCVSLAPNAPGSIDWELDAASHGGVDDTRELRDRAFYAPVQSRYRVFIVDEAHMVTTA GFNALLKIVEEPPEHLIFIFATTEPEKVLPTIRSRTHHYPFRLLPPRTMRALLARICEQEGVWDDAVYP LVI RAGGGSPRDTLSVLDQLLAGAADTHVTYTRALGLLGVTDVALI DDAVDALAACDAAALFGAI ESVI DGGHDPRRFATDLLERFRDLIVLQSVPDAASRGWDAPEDALDRMREQAARIGRATLTRYAEWQA GLGEMRGATAPRLLLEWCARLLLPSASDAESALLQRVERIETRLDMSIPAPQAVPRPSAAAAEPKHQ PAREPRPVLAPTPASSEPTVAAVRSMWPTVRDKVRLRSRTTEVMLAGATVRALEDNTLVLTHESAPL ARRLSEQRNADVLAEALKDALGVNWRVRCETGEPAAAASPVGGGANVATAKAVNPAPTANSTQRD EEEHMLAEAGRGDPSPRRDPEEVALELLQNELGARRIDNA

>Rv3783 - TB.seq 4229255:4230094 MW.32337 SEQ ID NO:283

MTFMDAQASFQTQSRTLARVRGDLVDGFRRHELWLHLGWQDIKQRYRRSVLGPFWITIATGTTAVA MGGLYSKLFRLELSEHLPYVTLGLIVWNLINAAILDGAEVFVANEGLIKQLPAPLSVHVYRLVWRQMIF FAHNIVIYFVIAIIFPKPWSWADLSFLPALALIFLNCVWVSLCFGILATRYRDIGPLLFSWQLLFFMTPII WNDETLRRQGAGRWSSIVELNPLLHYLDIVRAPLLGAHQELRHWLWLVLTWGWMLAAFAMRQYR ARVPYWV

>Rv3789 - TB.seq 4235371.4235733 MW:13378 SEQ ID NO:284 MRFWTGGLAGIVDFGLYWLYKVAGLQVDLSKAISFIVGTITAYLINRRWTFQAEPSTARFVAVMLLY GITFAVQVGLNHLCLALLHYRAWAIPVAFVIAQGTATVINFIVQRAVIFRIR

>Rv3790 - TB.seq 4235776:4237158 MW:50164 SEQ ID NO:285 MLSVGATTTATRLTGWGRTAPSVANVLRTPDAEMIVKAVARVAESGGGRGAIARGLGRSYGDNAQN GGGLVIDMTPLNTIHSIDADTKLVDIDAGVNLDQLMKAALPFGLWVPVLPGTRQVTVGGAIACDIHGK NHHSAGSFGNHVRSMDLLTADGEIRHLTPTGEDAELFWATVGGNGLTGIIMRATIEMTPTSTAYFIAD GDVTASLDETIALHSDGSEARYTYSSAWFDAISAPPKLGRAAVSRGRLATVEQLPAKLRSEPLKFDAP QLLTLPDVFPNGLANKYTFGPIGELWYRKSGTYRGKVQNLTQFYHPLDMFGEWNRAYGPAGFLQYQ FVIPTEAVDEFKKIIGVIQASGHYSFLNVFKLFGPRNQAPLSFPIPGWNICVDFPIKDGLGKFVSELDRR VLEFGGRLYTAKDSRTTAETFHAMYPRVDEWISVRRKVDPLRVFASDMARRLELL

>RV3791 - TB.seq 4237162:4237923 MW:27470 SEQ ID NO:286

MVLDAVGNPQTVLLLGGTSEIGLAICERYLHNSAARIVLACLPDDPRREDAAAAMKQAGARSVELIDF DALDTDSHPKMIEAAFSGGDVDVAIVAFGLLGDAEELWQNQRKAVQIAEINYTAAVSVGVLLAEKMR AQGFGQIIAMSSAAGERVRRANFVYGSTKAGLDGFYLGLSEALREYGVRVLVIRPGQVRTRMSAHLK EAPLTVDKEYVANLAVTASAKGKELVWAPAAFRYVMMVLRHIPRSIFRKLPI

>Rv3794 embA TB.seq 4243230:4246511 MW:115694 SEQ ID NO:287

VPHDGNERSHRIARLAAWSGIAGLLLCGIVPLLPVNQTTATIFWPQGSTADGNITQITAPLVSGAPRA LDISIPCSAIATLPANGGLVLSTLPAGGVDTGKAGLFVRANQDTVWAFRDSVAAVAARSTIAAGGCS ALHIWADTGGAGADFMGIPGGAGTLPPEKKPQVGGIFTDLKVGAQPGLSARVDIDTRFITTPGALKKA VMLLGVLAVLVAMVGLAALDRLSRGRTLRDWLTRYRPRVRVGFASRLADAAVIATLLLWHVIGATSS DDGYLLTVARVAPKAGYVANYΎRYFGTTEAPFDWYTSVLAQLAAVSTAGVWMRLPATLAGIACWLIV SRFVLRRLGPGPGGLASNRVAVFTAGAVFLSAWLPFNNGLRPEPLIALGVLVTWVLVERSIALGRLAP AAVAIIVATLTATLAPQGLIALAPLLTGARAIAQRIRRRRATDGLLAPLAVLAAALSLITWVFRDQTLATV AESARIKYKVGPTIAWYQDFLRYYFLTVESNVEGSMSRRFAVLVLLFCLFGVLFVLLRRGRVAGLASG PAWRLIGTTAVGLLLLTFTPTKWAVQFGAFAGLAGVLGAVTAFTFARIGLHSRRNLTLYVTALLFVLA WATSGINGWFYVGNYGVPWYDIQPVIASHPVTSMFLTLSILTGLLAAWYHFRMDYAGHTEVKDNRR NRILASTPLLWAVIMVAGEVGSMAKAAVFRYPLYTTAKANLTALSTGLSSCAMADDVLAEPDPNAGM LQPVPGQAFGPDGPLGGISPVGFKPEGVGEDLKSDPWSKPGLVNSDASPNKPNAAITDSAGTAGG KGPVGINGSHAALPFGLDPARTPVMGSYGENNLAATATSAWYQLPPRSPDRPLVWSAAGAIWSYK EDGDFIYGQSLKLQWGVTGPDGRIQPLGQVFPIDIGPQPAWRNLRFPLAWAPPEADVARIVAYDPNL SPEQWFAFTPPRVPVLESLQRLIGSATPVLMDIATAANFPCQRPFSEHLGIAELPQYRILPDHKQTAA SSNLWQSSSTGGPFLFTQALLRTSTIATYLRGDWYRDWGSVEQYHRLVPADQAPDAWEEGVITVP GWGRPGPIRALP

>Rv3795 embB TB.seq 4246511:4249804 MW:118023 SEQ ID NO:288

MTQCASRRKSTPNRAILGAFASARGTRWVATIAGLIGFVLSVATPLLPWQTTAMLDWPQRGQLGSV TAPLISLTPVDFTATVPCDWRAMPPAGGWLGTAPKQGKDANLQALFVWSAQRVDVTDRNWILS VPREQVTSPQCQRIEVTSTHAGTFANFVGLKDPSGAPLRSGFPDPNLRPQIVGVFTDLTGPAPPGLA VSATIDTRFSTRPTTLKLLAIIGAIVATWALIALWRLDQLDGRGSIAQLLLRPFRPASSPGGMRRLIPAS WRTFTLTDAWIFGFLLWHVIGANSSDDGYILGMARVADHAGYMSNYFRWFGSPEDPFGWYΎNLLA LMTHVSDASLWMRLPDLAAGLVCWLLLSREVLPRLGPAVEASKPAYWAAAMVLLTAWMPFNNGLR PEGIIALGSLVTYVLIERSMRYSRLTPAALAWTAAFTLGVQPTGLIAVAALVAGGRPMLRILVRRHRLV GTLPLVSPMLAAGTVILTWFADQTLSTVLEATRVRAKIGPSQAWYTENLRYYYLILPTVDGSLSRRFG FLITALCLFTAVFIMLRRKRIPSVARGPAWRLMGVIFGTMFFLMFTPTKWVHHFGLFAAVGAAMAALT TVLVSPSVLRWSRNRMAFLAALFFLLALCWATTNGWWYVSSYGVPFNSAMPKIDGITVSTIFFALFAI AAGYAAWLHFAPRGAGEGRLIRALTTAPVPIVAGFMAAVFVASMVAGIVRQYPTYSNGWSNVRAFV GGCGLADDVLVEPDTNAGFMKPLDGDSGSWGPLGPLGGVNPVGFTPNGVPEHTVAEAIVMKPNQP GTDYDWDAPTKLTSPGINGSTVPLPYGLDPARVPLAGTYTTGAQQQSTLVSAWYLLPKPDDGHPLV WTAAGKIAGNSVLHGYTPGQTWLEYAMPGPGALVPAGRMVPDDLYGEQPKAWRNLRFARAKMP ADAVAVRWAEDLSLTPEDWIAVTPPRVPDLRSLQEYVGSTQPVLLDWAVGLAFPCQQPMLHANGIA EIPKFRITPDYSAKKLDTDTWEDGTNGGLLGITDLLLRAHVMATYLSRDWARDWGSLRKFDTLVDAP PAQLELGTATRSGLWSPGKIRIGP

>Rv3834c serS seryl-tRNA synthase TB.seq 4307655:4308911 MW:45293 SEQ ID NO:289

VIDLKLLRENPDAVRRSQLSRGEDPALVDALLTADAARRAVISTADSLRAEQKAASKSVGGASPEERP PLLRRAKELAEQVKAAEADEVEAEAAFTAAHLAISNVIVDGVPAGGEDDYAVLDWGEPSYLENPKD HLELGESLGLIDMQRGAKVSGSRFYFLTGRGALLQLGLLQLALKLAVDNGFVPTIPPVLVRPEVMVGT GFLGAHAEEVYRVEGDGLYLVGTSEVPLAGYHSGEILDLSRGPLRYAGWSSCFRREAGSHGKDTRG IIRVHQFDKVEGFVYCTPADAEHEHERLLGWQRQMLARIEVPYRVIDVAAGDLGSSAARKFDCEAWI PTQGAYRELTSTSNCTTFQARRLATRYRDASGKPQIAATLNGTLATTRWLVAILENHQRPDGSVRVP DALVPFVGVEVLEPVA >Rv3907c pcnA polynucleotide polymerase TB.seq 4391631:4393070 MW:53057 SEQ ID NO:290

VPEAVQEADLLTAAAVALNRHAALLRELGSVFAAAGHELYLVGGSVRDALLGRLSPDLDFTTDARPE RVQEIVRPWADAVWDTGIEFGTVGVGKSDHRMEITTFRADSYDRVSRHPEVRFGDCLEGDLVRRDF TTNAMAVRVTATGPGEFLDPLGGLAALRAKVLDTPAAPSGSFGDDPLRMLRAARFVSQLGFAVAPR VRAAIEEMAPQLARISAERVAAELDKLLVGEDPAAGIDLMVQSGMGAWLPEIGGMRMAIDEHHQHK DVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDIGKPATRRHEPDGGVSFHHHEWGAKMVRKRMR ALKYSKQMIDDISQLVYLHLRFHGYGDGKWTDSAVRRYVTDAGALLPRLHKLVRADCTTRNKRRAAR LQASYDRLEERIAELAAQEDLDRVRPDLDGNQIMAVLDIPAGPQVGEAWRYLKELRLERGPLSTEEA TTELLSWWKSRGNR

A number of embodiments of the invention have been described. Nevertheless, it will be understood that various modifications may be made without departing from the spirit and scope of the invention. Accordingly, other embodiments are within the scope of the following claims.

Claims

WHAT IS CLAIMED IS:

1. A method for identifying a nucleic acid or a polypeptide sequence that may be a target for a drug comprising the following steps:

(a) providing a first nucleic acid or a polypeptide sequence that is known to be a drag target;

(b) providing at least one algorithm selected from the group consisting of a "domain fusion" method, a "phylogenetic profile" method and a "physiologic linkage" method, wherein the algorithm is capable analyzing a functional relationship between nucleic acid or polypeptide sequences; and (c) comparing the first nucleic acid or the polypeptide drug target sequence to a plurality of sequences using at least one of the algorithms as set forth in step (b) to identify a second sequence that has a functional relationship to the first sequence, thereby identifying a nucleic acid or a polypeptide sequence that may be a target for a drag .

2. A method for identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism comprising the following steps: (a) providing a first nucleic acid or a polypeptide sequence that is known to be essential for the growth or viability of an organism;

(b) providing at least one algorithm capable analyzing a functional relationship between nucleic acid or polypeptide sequences selected from the group consisting of a

"domain fusion" method, a "phylogenetic profile" method and a "physiologic linkage" method; and

(c) comparing the first nucleic acid or the polypeptide sequence to a plurality of sequences using at least one of the algorithms as set forth in step (b) to identify a second sequence that has a functional relationship to the first sequence, thereby identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism.

3. The method of claim 1 or claim 2, wherein the drag is an anti- microbial drug.

4. The method of claim 1 or claim 2, wherein the first nucleic acid or a polypeptide sequence is derived from a pathogen.

5. The method of claim 4, wherein the pathogen is a microorganism.

6. The method of claim 1 or claim 2, wherein the microorganism is Mycobacterium tuberculosis (MTB).

7. The method of claim 1 or claim 2, wherein the plurality of sequences used to identify a second sequence comprises a database of the gene sequences of an entire genome of an organism.

8. The method of claim 1 or claim 2, wherein the plurality of sequences used to identify a second sequence comprises a database of the gene sequences derived from a pathogen.

9. The method of claim 1 or claim 2, wherein the "phylogenetic profile" method algorithm comprises

(a) obtaining data, comprising a list of proteins from at least two genomes; (b) comparing the list of proteins to form a protein phylogenetic profile for each protein, wherein the protein phylogenetic profile indicates the presence or absence of a protein belonging to a particular protein family in each of the at least two genomes based on homology of the proteins; and

(c) grouping the list of proteins based on similar profiles, wherein proteins with similar profiles are indicated to have a functional relationship.

10. The method of claim 9, wherein the phylogenetic profile is in the form of a vector, matrix or phylogenetic tree.

11. The method of claim 9, comprising determining the significance of homology between the proteins by computing a probability (p) value threshold.

12. The method of claim 11 , wherein the probability is set with respect to the value 1/NM, based on the total number of sequence comparisons that are to be performed, wherein N is the number of proteins in the first organism's genome and Min all other genomes.

13. The method of claim 9, wherein the presence or absence is by calculating an evolutionary distance.

14. The method of claim 13, wherein the evolutionary distance is calculated by:

(a) aligning two sequences from the list of proteins;

(b) determining an evolution probability process by constructing a conditional probability matrix: p(aa— aa'), where aa and aa' are any amino acids, said conditional probability matrix being constracted by converting an amino acid substitution matrix from a log odds matrix to said conditional probability matrix;

(c) accounting for an observed alignment of the constracted conditional probability matrix by taking the product of the conditional probabilities for each aligned pair during the alignment of the two sequences, represented by

; and n (d) determining an evolutionary distance α from powers equation p'=p^α(aa→aa'), maximizing for P.

15. The method of claim 14, wherein the conditional probability matrix is defined by a Markov process with substitution rates, over a fixed time interval.

16. The method of claim 14, where the conversion from an amino acid substitution matrix to a conditional probability matrix is represented by:

BLOSUM62._J PB(Ϊ →j) =p(f)2^A > where BLOSUM62 is an amino acid substitution matrix, and P(i->j) is the probability that amino acid i is replaced by amino acidy through point mutations according to BLOSUM62 scores.

17. The method of claim 16, where 's are the abundances of amino acid j and are computed by solving a plurality of linear equations given by the normalization condition that:

∑A(i → ) = l .

18. The method of claim 1 or claim 2, wherein the "physiologic linkage" method algorithm identifies proteins and nucleic acids that participate in a common functional pathway.

19. The method of claim 1 or claim 2, wherein the "physiologic linkage" method algorithm comprises identifies proteins and nucleic acids that participate in the synthesis of a common stractural complex.

20. The method of claim 1 or claim 2, wherein the "physiologic linkage" method algorithm comprises identifies proteins and nucleic acids that participate in a common metabolic pathway.

21. The method of claim 1 or claim 2, wherein the "domain fusion" method algorithm comprises

(a) aligning a first primary amino acid sequence of multiple distinct non-homologous polypeptides to second primary amino acid sequence of a plurality of proteins; and (b) for any alignment found between the first primary amino acid sequences of all of such multiple distinct non-homologous polypeptides and at least one protein of the second primary amino acid sequences, outputting an indication identifying the aligned second primary amino acid sequence as an indication of a functional link between the aligned first and second polypeptide sequences.

22. The method of claim 21 , wherein the aligning is performed by an algorithm selected from the group consisting of a Smith- Waterman algorithm, Needleman- Wunsch algorithm, a BLAST algorithm, a FASTA algorithm, and a PSI-BLAST algorithm.

23. The method of claim 21 , wherein the multiple distinct non- homologous polypeptides are obtained by translating a nucleic acid sequence from a genome database.

24. The method of claim 21 , wherein the plurality of proteins have a known function.

25. The method of claim 21 , wherein at least one of the multiple distinct non-homologous polypeptides has a known function.

26. The method of claim 21 , wherein at least one of the multiple distinct non-homologous polypeptides has an unknown function.

27. The method of claim 21, wherein the alignment is based on the degree of homology of the multiple distinct non-homologous polypeptides to the plurality of proteins.

28. The method of claim 21 , further comprising determining the significance of the aligned and identified second primary amino acid sequence by computing a probability (p) value threshold.

29. The method of claim 28, wherein the probability threshold is set with respect to the value 1/NM, based on the total number of sequence comparisons that are to be performed, wherein N is the number of proteins in a first organism's genome and Min all other genomes.

30. The method of claim 21, further comprising filtering excessive functional links between one first primary amino acid sequence of multiple distinct non- homologous polypeptides and an excessive number of other distinct non-homologous polypeptides for any alignment found between the first primary amino acid sequences of the distinct non-homologous polypeptides and at least one of the second primary amino acid sequences of the plurality of proteins.

31. A computer program product, stored on a computer-readable medium, for identifying a nucleic acid or a polypeptide sequence that may be a target for a drug, the computer program product comprising instructions for causing a computer system to be capable of: (a) inputting a first nucleic acid or a polypeptide sequence that is known to be a drag target;

(b) accessing at least one algorithm capable analyzing a functional relationship between nucleic acid or polypeptide sequences selected from the group consisting of a "domain fusion" method, a "phylogenetic profile" method and a "physiologic linkage" method; and

(c) comparing the first nucleic acid or the polypeptide drag target sequence to a plurality of sequences using at least one of the algorithms set forth in step (b) to identify a second sequence that has a functional relationship to the first sequence and generating an output identifying a nucleic acid or a polypeptide sequence that may be a target for a drug .

32. A computer program product, stored on a computer-readable medium, for identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism, the computer program product comprising instructions for causing a computer system to be capable of: (a) providing a first nucleic acid or a polypeptide sequence that is known to be essential for the growth or viability of an organism;

(b) accessing at least one algorithm capable analyzing a functional relationship between nucleic acid or polypeptide sequences selected from the group consisting of a "domain fusion" method, a "phylogenetic profile" method and a "physiologic linkage" method; and (c) comparing the first nucleic acid or the polypeptide sequence to a plurality of sequences using at least one of the algorithms set forth in step (b) to identify a second sequence that has a functional relationship to the first sequence and generating an output identifying a nucleic acid or a polypeptide sequence that may be essential for the growth or viability of an organism.

33. A computer system, comprising:

(a) a processor; and

(b) a computer program product as set forth in claim 31 or claim 32.