WO1999023227A3 - Hyaluronan synthase gene and uses thereof - Google Patents

Hyaluronan synthase gene and uses thereof Download PDF

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Publication number
WO1999023227A3
WO1999023227A3 PCT/US1998/023153 US9823153W WO9923227A3 WO 1999023227 A3 WO1999023227 A3 WO 1999023227A3 US 9823153 W US9823153 W US 9823153W WO 9923227 A3 WO9923227 A3 WO 9923227A3
Authority
WO
WIPO (PCT)
Prior art keywords
synthase gene
hyaluronan synthase
hyaluronate
nucleic acid
segment
Prior art date
Application number
PCT/US1998/023153
Other languages
French (fr)
Other versions
WO1999023227A9 (en
WO1999023227A2 (en
Inventor
Paul H Weigel
Paul Deangelis
Kshama Kumari
Original Assignee
Univ Oklahoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to CA2307842A priority Critical patent/CA2307842C/en
Application filed by Univ Oklahoma filed Critical Univ Oklahoma
Priority to BRPI9814834-6A priority patent/BR9814834B1/en
Priority to DK98957450T priority patent/DK1025211T3/en
Priority to EP98957450A priority patent/EP1025211B1/en
Priority to KR1020007004743A priority patent/KR20010031682A/en
Priority to AU13706/99A priority patent/AU762036B2/en
Priority to DE69828193T priority patent/DE69828193T2/en
Priority to AT98957450T priority patent/ATE284954T1/en
Priority to JP2000519083A priority patent/JP4731013B2/en
Publication of WO1999023227A2 publication Critical patent/WO1999023227A2/en
Publication of WO1999023227A3 publication Critical patent/WO1999023227A3/en
Publication of WO1999023227A9 publication Critical patent/WO1999023227A9/en
Priority to AU2003248204A priority patent/AU2003248204A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A61K39/102—Pasteurellales, e.g. Actinobacillus, Pasteurella; Haemophilus
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C08B37/0072—Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/836—Bacillus licheniformis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Polymers & Plastics (AREA)
  • Materials Engineering (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Biophysics (AREA)
  • Communicable Diseases (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a nucleic acid segment having a coding region segment encoding enzymatically active Streptococcus equisimilis hyaluronate synthase (seHAS), and to the use of this nucleic acid segment in the preparation of recombinant cells which produce hyaluronate synthase and its hyaluronic acid product. Hyaluronate is also known as hyaluronic acid or hyaluronan.
PCT/US1998/023153 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof WO1999023227A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
AU13706/99A AU762036B2 (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof
BRPI9814834-6A BR9814834B1 (en) 1997-10-31 1998-10-30 recombinant host cell.
DK98957450T DK1025211T3 (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof
EP98957450A EP1025211B1 (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof
KR1020007004743A KR20010031682A (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof
CA2307842A CA2307842C (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof
DE69828193T DE69828193T2 (en) 1997-10-31 1998-10-30 HYALURONAN SYNTHASE GENE AND ITS USE
AT98957450T ATE284954T1 (en) 1997-10-31 1998-10-30 HYALURONAN SYNTHASE GENE AND ITS USE
JP2000519083A JP4731013B2 (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and use thereof
AU2003248204A AU2003248204A1 (en) 1997-10-31 2003-09-19 Hyaluronan Synthase Gene and uses Thereof

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US6443597P 1997-10-31 1997-10-31
US60/064,435 1997-10-31
US17885198A 1998-10-26 1998-10-26
US09/178,851 1998-10-26

Publications (3)

Publication Number Publication Date
WO1999023227A2 WO1999023227A2 (en) 1999-05-14
WO1999023227A3 true WO1999023227A3 (en) 1999-08-19
WO1999023227A9 WO1999023227A9 (en) 2000-07-13

Family

ID=26744517

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/023153 WO1999023227A2 (en) 1997-10-31 1998-10-30 Hyaluronan synthase gene and uses thereof

Country Status (13)

Country Link
US (10) US6833264B1 (en)
EP (2) EP1522579A3 (en)
JP (1) JP4731013B2 (en)
KR (1) KR20010031682A (en)
CN (1) CN1322121C (en)
AT (1) ATE284954T1 (en)
AU (2) AU762036B2 (en)
BR (1) BR9814834B1 (en)
CA (1) CA2307842C (en)
DE (1) DE69828193T2 (en)
DK (1) DK1025211T3 (en)
ES (1) ES2235378T3 (en)
WO (1) WO1999023227A2 (en)

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EP1522579A3 (en) * 1997-10-31 2007-11-07 The Board Of Regents Of The University Of Oklahoma Hyaluronan synthase gene and uses thereof
US7060469B2 (en) * 1998-04-02 2006-06-13 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
CN1327894C (en) * 1998-04-02 2007-07-25 俄克拉何马大学董事会 Nucleic acid encoding hyaluronan synthase and method of use
US7534589B2 (en) * 1999-11-10 2009-05-19 The Board Of Regents Of The University Of Oklahoma Polymer grafting by polysaccharide synthases
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US20080274999A1 (en) * 2005-01-03 2008-11-06 Novozymes Biopolymer A/S Hyaluronic Acid Fraction with Moisturizing and Anti-Wrinkle Properties
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