WO1998026644A3 - Confirmatory assays for small molecule drugs - Google Patents

Confirmatory assays for small molecule drugs Download PDF

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Publication number
WO1998026644A3
WO1998026644A3 PCT/US1998/006098 US9806098W WO9826644A3 WO 1998026644 A3 WO1998026644 A3 WO 1998026644A3 US 9806098 W US9806098 W US 9806098W WO 9826644 A3 WO9826644 A3 WO 9826644A3
Authority
WO
WIPO (PCT)
Prior art keywords
analyte
confirmatory
interfering substance
assay
neutralizing antibody
Prior art date
Application number
PCT/US1998/006098
Other languages
French (fr)
Other versions
WO1998026644A2 (en
WO1998026644A9 (en
Inventor
Jeffrey E Shindelman
Original Assignee
Boehringer Mannheim Corp
Jeffrey E Shindelman
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Corp, Jeffrey E Shindelman filed Critical Boehringer Mannheim Corp
Priority to DE1066523T priority Critical patent/DE1066523T1/en
Priority to EP98915179A priority patent/EP1066523A2/en
Priority to PCT/US1998/006098 priority patent/WO1998026644A2/en
Publication of WO1998026644A2 publication Critical patent/WO1998026644A2/en
Publication of WO1998026644A3 publication Critical patent/WO1998026644A3/en
Publication of WO1998026644A9 publication Critical patent/WO1998026644A9/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The invention relates to an assay system for the improved detection of analytes, and the ability to distinguish them from cross-reacting substances. Samples giving a positive reaction in a direct assay test are treated with a neutralizing antibody that inhibits reactivity of the true analyte, but not the interfering substance. In bidirectional antibody type confirmatory assays, the neutralizing antibody binds the analyte from a different orientation, and does not impede other reagents in the assay mixture. In adsorption type confirmatory assays, the neutralizing antibody is provided in an amount sufficient to adsorb the analyte but not all of the interfering substance. When retested in an immunoassay, the neutralized sample gives a negative result if it originally contained the true analyte. Samples giving a positive reaction in both the direct and confirmatory tests are marked as containing an interfering substance. The confirmatory assay easily distinguishes the true analyte and reduces the rate of false positives, even when the interfering substance is unknown and present at high concentration.
PCT/US1998/006098 1998-03-27 1998-03-27 Confirmatory assays for small molecule drugs WO1998026644A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE1066523T DE1066523T1 (en) 1998-03-27 1998-03-27 CONTROL DETERMINATION METHODS FOR DRUGS CONSTRUCTED FROM SMALL MOLECULES
EP98915179A EP1066523A2 (en) 1998-03-27 1998-03-27 Confirmatory assays for small molecule drugs
PCT/US1998/006098 WO1998026644A2 (en) 1998-03-27 1998-03-27 Confirmatory assays for small molecule drugs

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US1998/006098 WO1998026644A2 (en) 1998-03-27 1998-03-27 Confirmatory assays for small molecule drugs

Publications (3)

Publication Number Publication Date
WO1998026644A2 WO1998026644A2 (en) 1998-06-25
WO1998026644A3 true WO1998026644A3 (en) 1999-02-18
WO1998026644A9 WO1998026644A9 (en) 1999-04-08

Family

ID=22266698

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/006098 WO1998026644A2 (en) 1998-03-27 1998-03-27 Confirmatory assays for small molecule drugs

Country Status (3)

Country Link
EP (1) EP1066523A2 (en)
DE (1) DE1066523T1 (en)
WO (1) WO1998026644A2 (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2775299A (en) * 1998-02-20 1999-09-06 Microgenics Corporation Immunoassays for determination of lsd and lsd metabolites
US6548645B1 (en) * 1999-06-18 2003-04-15 Microgenetics Corporation Immunoassay for 2-oxo-3-hydroxy LSD
GB0027064D0 (en) * 2000-11-06 2000-12-20 Randox Lab Ltd Multi-analyte immunoassay
US7029918B2 (en) * 2002-01-25 2006-04-18 Roche Diagnostics Operations, Inc. Water-soluble derivatives of lipophilic drugs
GB2409038B (en) * 2002-10-09 2006-04-05 Waters Investments Ltd Methods and apparatus for identifying compounds in a sample
SG187787A1 (en) * 2010-08-10 2013-03-28 Amgen Inc Dual function in vitro target binding assay for the detection of neutralizing antibodies against target antibodies
CN102662057A (en) * 2012-06-07 2012-09-12 广州易航生物科技有限公司 Cloned enzyme-donor immunoassay (CEDIA) ImmunoChip drug detecting kit
US9618523B2 (en) * 2013-02-28 2017-04-11 Siemens Healthcare Diagnostics Inc. Methods and reagents for determining isomeric analytes
US20230219889A1 (en) 2020-05-19 2023-07-13 Cybin Irl Limited Deuterated tryptamine derivatives and methods of use

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0353614A2 (en) * 1988-08-04 1990-02-07 Abbott Laboratories Confirmatory assay for Chlamydia trachomatis
EP0399184A2 (en) * 1989-04-10 1990-11-28 Abbott Laboratories Reagents, methods and kits for an amphetamine-class fluorescence polarization immunoassay
WO1997019100A1 (en) * 1995-11-20 1997-05-29 Boehringer Mannheim Corporation N-1-carboxyalkyl derivatives of lsd
EP0816364A1 (en) * 1996-07-02 1998-01-07 F. Hoffmann-La Roche Ag Reagents for lysergic acid diethylamide immunoassay
EP0820984A1 (en) * 1996-07-25 1998-01-28 Roche Diagnostics GmbH Activated amphetamines

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0353614A2 (en) * 1988-08-04 1990-02-07 Abbott Laboratories Confirmatory assay for Chlamydia trachomatis
EP0399184A2 (en) * 1989-04-10 1990-11-28 Abbott Laboratories Reagents, methods and kits for an amphetamine-class fluorescence polarization immunoassay
WO1997019100A1 (en) * 1995-11-20 1997-05-29 Boehringer Mannheim Corporation N-1-carboxyalkyl derivatives of lsd
EP0816364A1 (en) * 1996-07-02 1998-01-07 F. Hoffmann-La Roche Ag Reagents for lysergic acid diethylamide immunoassay
EP0820984A1 (en) * 1996-07-25 1998-01-28 Roche Diagnostics GmbH Activated amphetamines

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
RADCLIFFE W A ET AL: "RADIOIMMUNOASSAY OF LYSERGIC ACID DIETHYLAMIDE (LSD) IN SERUM AND URINE BY USING ANTISERA OF DIFFERENT SPECIFICITIES", CLINICAL CHEMISTRY, vol. 23, no. 2, 1977, pages 169 - 174, XP002041570 *

Also Published As

Publication number Publication date
WO1998026644A2 (en) 1998-06-25
DE1066523T1 (en) 2001-09-20
EP1066523A2 (en) 2001-01-10
WO1998026644A9 (en) 1999-04-08

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