WO1997001330A1 - Novel adjuvant compositions and vaccine formulations comprising same - Google Patents

Novel adjuvant compositions and vaccine formulations comprising same Download PDF

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Publication number
WO1997001330A1
WO1997001330A1 PCT/US1996/011051 US9611051W WO9701330A1 WO 1997001330 A1 WO1997001330 A1 WO 1997001330A1 US 9611051 W US9611051 W US 9611051W WO 9701330 A1 WO9701330 A1 WO 9701330A1
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Prior art keywords
polysaccharide
adjuvant
phospholipid
antigen
animal
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PCT/US1996/011051
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French (fr)
Inventor
Indu Parikh
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Research Triangle Pharmaceuticals
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Application filed by Research Triangle Pharmaceuticals filed Critical Research Triangle Pharmaceuticals
Priority to JP50458697A priority Critical patent/JP2001523216A/en
Priority to EP96923519A priority patent/EP0857059A1/en
Priority to AU64007/96A priority patent/AU6400796A/en
Publication of WO1997001330A1 publication Critical patent/WO1997001330A1/en

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/39—Medicinal preparations containing antigens or antibodies characterised by the immunostimulating additives, e.g. chemical adjuvants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
    • A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
    • A61K47/544—Phospholipids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/16—Antivirals for RNA viruses for influenza or rhinoviruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55555—Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/555—Medicinal preparations containing antigens or antibodies characterised by a specific combination antigen/adjuvant
    • A61K2039/55511—Organic adjuvants
    • A61K2039/55583—Polysaccharides

Definitions

  • the present invention relates to adjuvant compositions and to vaccine formulations comprising same, as well as to methods of making and using such adjuvants and vaccines.
  • an antigen into an animal has long been regarded as an effective method of producing antisera (i.e., serum antibody) or increasing the antisera levels in the animal either for the protection of the host animal (i.e., vaccination) or to produce antisera for isolation and use in other animals.
  • antisera i.e., serum antibody
  • Vaccines occupy a unique place in health care because unlike most therapies they are given to healthy people to prevent diseases. Because vaccination use has been a primary factor in controlling many childhood diseases, great effort is applied in expanding the use of vaccines. Vaccines are being developed for many diseases including cholera, malaria, herpes, chicken pox, and pneumonia.
  • Freund's complete adjuvant and Freund's incomplete adjuvant are the emulsification of the antigen in mineral oil to ensure the formation of a slow-release depot of the antigen at the injection at the injection site.
  • Freund's complete adjuvant contains killed mycobacteria and apparently acts by preferentially inducing antibody against epitopes on denatured proteins. The result is higher levels of antisera produced when compared with antigen alone.
  • Freund's complete and incomplete adjuvant are known to produce significant toxic complications.
  • FCA In addition to causing chronic pain and suffering as an undesirable side effect, FCA induces local granulomas and possibly malignancies. For these reasons, FCA has never been approved for use in human or veterinary vaccines in the United States.
  • a major goal in the area of vaccine development is the production of vaccine formulations which include the efficacy and exclude the deleterious side effects of adjuvants such as FCA. Attempts to reduce the toxicity while retaining efficacy of adjuvants such as Freund's adjuvant have largely failed, in part, from a lack of understanding of the specific biological mechanism(s) responsible for adjuvant efficacy.
  • the original adjuvants were substances of biological origin that enhanced a specific antibody response. Since mycobacterium has been effectively used as an adjuvant, attempts have been made to isolate those biologically active components from mycobacterium cell walls that are responsible for immunostimulation.
  • the lipid fraction extracted from mycobacteria contains trehalose dimycolate (TDM) as an active component while the mycobacterium cell wall contains N-acetylmuramyl-L-alanine-D- isoglutamine, also known as muramyl dipeptide (MDP), as an active component.
  • TDM trehalose dimycolate
  • MDP muramyl dipeptide
  • Glucan a ⁇ -1, 3-polyglucose from Saccharomyces cerevisiae, a yeast, has been reported to induce antitumor effects, improve resistance to microbial pathogens and stimulate antibody response to a variety of antigens.
  • nonmicrobial substances include detergents, salts, sugars, polyribonucleotides, and natural substances of mammalian origin. Both nonionic and cationic detergents have achieved success as adjuvants, with more lipophilic detergents being more effective. Saponins have amphipathic surface activity, so their mechanism for inducing adjuvant activity may be similar to that of detergents. Saponins are not used in human vaccines because of toxicological issues. Lymphokines and monokines have a very short biological half-life, so pharmacokinetic concerns preclude their use as adjuvants.
  • a clear distinction between the vehicle and the adjuvant cannot always be made because many vehicles have adjuvant-like activity, which may result from immunostimulation effects and/or slow release of antigen.
  • aluminum salts are the most widely used vehicles in vaccines licensed for human and veterinary use. The antigen is believed to reside in the aluminum gel, releasing slowly over time to produce a continual challenge to the immune system. In addition to this clear vehicle effect, aluminum salts probably act as true adjuvants by virtue of their chemotactic properties for various immunological cells.
  • vehicles with adjuvant-like activities include water/oil emulsions, oil/water emulsions, microencapsulation, and liposomes.
  • liposomes For more than 20 years, the ability of liposomes to stimulate antibody response has been known, but issues in the development of appropriate components for liposomes as carriers for vaccines are still in debate.
  • the antigen can be encapsulated into the aqueous spaces of the liposome core or attached to the external surface of the lipid bilayer.
  • the adjuvant property of liposomes can be further enhanced by the inclusion of certain immunostimulants such as lipid A, lipopolysaccharide, or MDP. Liposomes are believed to exhibit their adjuvant properties by being taken up preferentially by macrophages, but liposomal delivery does not provide for a sustained release of antigen.
  • Nanoparticles solid colloidal particles from 10 to 1000 nm of synthetic polymers such as polymethylmethacrylate, are reported to be effective adjuvants whether the antigen is encapsulated within the nanoparticle or adsorbed to the nanoparticle surface.
  • the adjuvant effect of these vaccines improves with increasing hydrophobicity and decreasing particle size. Because the polymethylmethacrylate nanoparticles are slowly biodegradable, the adjuvant effect may be caused by a continuing antigen challenge to the immune system.
  • U.S. Patent 5,273,965 describes compounds of the saponin family which can be used to administer vaccines via nasal spray or eye drops.
  • This unit ligand composition a heptaglucoside
  • 2-aminopyridine to increase the capacity of the glucocide to stimulate ⁇ -glucan receptors and potentiate functions mediated by such receptors.
  • the heptaglucoside is described as useful for vaccine or other immunomodulating agent preparations such as adjuvant therapy.
  • U.S. Patent 5, 189,028 to L.H. Nikl, et al. describes the stimulation of immune systems of fish by administration of a ⁇ -1, 3-glucan, particularly a ⁇ -1, 3-glucan having a ⁇ -1, 3-linked main chain with ⁇ -1, 6-linked single glucose side chains.
  • U.S. Patent 4,981,684 to N.M. MacKenzie, et al. discloses the formulation of adjuvant matrices comprising a water-insoluble antigen which is solubilized with a solubilizing agent, e.g., a detergent species, urea or guanidine, then admixed with a glycoside, a sterol, and optionally, a phospholipid, thereby forming an immuno-stimulating complex substantially without removal of the solubilizing agent.
  • a solubilizing agent e.g., a detergent species, urea or guanidine
  • U.S. Patent 5,032,401 to S. Jamas describes a pharmaceutical composition comprising whole glucan particles and a pharmacologically active substance such as a drug or antigen contained within, uniformly dispersed with, or chemically linked to the whole glucan particles.
  • U.S. Patents 5,091,187 and 5,091,188 to D.H. Haynes disclose phospholipid-coated microcrystal or microparticle compositions providing an injectible delivery form for administration of water-insoluble drugs to a mammalian host for sustained release.
  • the pharmaceutically effective agent is produced in solid form coated with a membrane-forming lipid which stabilizes the active ingredient material by hydrophobic and hydrophilic interactions.
  • the active solid ingredient-containing particles are formed in small finely divided form, by sonication or other process inducing high shear.
  • U.S. Patent 5,246,707 discloses the sustained release delivery of water-soluble biomolecules and drugs using phospholipid-coated microcrystals, microdroplets and high-concentration liposomes. The phospholipid-coated microcrystal and the phospholipid-coated microdroplet are described as useable as vaccine adjuvants.
  • It is another object of the present invention to provide a vaccine composition comprising such adjuvant which is safe and effective in use.
  • the present invention relates to an adjuvant composition, which may be usefully employed with an antigen or an antigen-based vaccine, to enhance immunostimulative response.
  • the invention relates to an adjuvant comprising a polysaccharide-phospholipid conjugate.
  • Particularly preferred polysaccharides of the adjuvant of the invention include ⁇ -glucan, chitosan, galactomanans, and alginates.
  • the present invention relates to a method of synthesizing an adjuvant from a polysaccharide.
  • the adjuvant may be synthesized from a polysaccharide and phospholipid, using any suitable reagents, including bifunctional or other polyfunctional reagents.
  • the adjuvant may for example be synthesized by the steps of:
  • the invention in a further aspect comprises a vaccine composition including the adjuvant of the invention and an antigen for producing antibodies in an animal.
  • the invention relates to inducing an immunological response in an animal comprising administering the vaccine including the adjuvant in an amount sufficient to produce an antibody response in such animal.
  • FIGURE 1 is a graph of the antibody titer in mice, for vaccination with bovine serum albumin (BSA), BSA in microdroplet form (BSA-MD), BSA in microdroplet form with a ⁇ -glucan conjugate adjuvant according to one embodiment of the present invention (BSA-GMD), and BSA with Freund's
  • BSA bovine serum albumin
  • BSA-MD BSA in microdroplet form
  • BSA-GMD BSA in microdroplet form with a ⁇ -glucan conjugate adjuvant according to one embodiment of the present invention
  • BSA-GMD BSA with Freund's
  • FCA Complete Adjuvant
  • FIGURE 2 is a graph of IgG titer in mice plasma at 1:4096 dilution, at one, two and three months after injection, for vaccination with bovine serum albumin (BSA), BSA in microdroplet form with a ⁇ -glucan conjugate adjuvant according to one embodiment of the present invention (BSA-MD), and BSA with Freund's Complete Adjuvant (BSA-Freund's).
  • BSA bovine serum albumin
  • BSA-MD BSA in microdroplet form with a ⁇ -glucan conjugate adjuvant according to one embodiment of the present invention
  • BSA-Freund's BSA with Freund's Complete Adjuvant
  • the present invention is based on the surprising and unexpected discovery that polysaccharides when conjugated with phospholipids form adjuvants which (i) increase the titer, duration, isotype and avidity of the antibody produced in a host animal, and (ii) have low toxicity and good effectiveness and safety characteristics in the host animal when compared to Freund's adjuvant.
  • the polysaccharides which are used to form the adjuvants of the invention may comprise any suitable polysaccharides, e.g., a polysaccharide having an immunostimulative activity.
  • Polysaccharides which may be used in adjuvant compositions in the broad practice of the present invention include species described in "Carbohydrate Chemistry,” ed. by John F. Kennedy, Clarendon Press, Oxford, 1988; “The Carbohydrates, Chemistry and Biochemistry,” ed. by W. Pigman and D. Horton, Academic Press, Inc., 1970; and “Chitin, Chitosan, and Related Enzymes,” ed. by John P. Zikakis, Academic Press, Inc., 1984.
  • Particularly preferred polysaccharide species include ⁇ -glucans, chitosan, galactomanans, and alginates, with ⁇ -glucans being currently most preferred.
  • the adjuvants of the present invention may be synthesized from a polysaccharide and phospholipid, via any suitable synthetic method, and using any suitable reagents, including bifunctional or other polyfunctional reagents.
  • the polysaccharide is complexed by conjugation with a phospholipid by reaction, with may comprise oxidation of the polysaccharide, or other functionalizing reaction, to produce a functionalized polysaccharide which is of a form that is conjugatable with a phospholipid.
  • the adjuvant may for example be synthesized by reacting the polysaccharide with an oxidizing agent to form aldehyde functionality on the polysaccharide, following which the aldehyde-functionalized polysaccharide is reacted with an appropriate bifunctional reagent, to yield a polysaccharide functionalized with a linking functionality.
  • the linking functionality is reactive with a selected phospholipid to further yield a polysaccharide- phospholipid conjugate.
  • the bifunctional reagent in the above-described synthesis method comprises a thiol hydrazide compound, which is employed in the synthesis procedure to yield a thiol-functionalized polysaccharide.
  • the thiol- functionalized polysaccharide subsequently is reacted with a phospholipid, to yield a polysaccharide-phospholipid conjugate as the aforementioned adjuvant.
  • the starting polysaccharide is reacted with an oxidizing reagent such as a periodate compound, to convert oxidizable functional groups of the polysaccharide to corresponding aldehyde functionality (-CHO pendant groups).
  • an oxidizing reagent such as a periodate compound
  • the resulting aldehyde-functionalized polysaccharide then is reacted with a mercaptohydrazide compound, such as for example 2-acetamido-4-mercapto-butyric acid hydrazide (AMBH), or other suitable bifunctional reagent, to provide a suitable reactive moiety (end group) on the functionalized polysaccharide for linking of a phospholipid conjugate thereto.
  • AMBH 2-acetamido-4-mercapto-butyric acid hydrazide
  • reagents other than the bifunctional reagents described in the preceding paragraph may be advantageously employed, including for example reagents which may not require initial oxidation of the polysaccharide.
  • a phospholipid may be conjugated to an existing functional group on the polysaccharide, such as an amino or a hydroxyl function, by methods known in the art of synthetic chemistry.
  • the phospholipid conjugate which is used to form the polysaccharide- phospholipid adjuvant of the present invention may comprise any suitable phospholipid, which is coordinatable, e.g., by covalent, ionic, hydrogen, associative, or other conjugative bonding, to form a pharmacologically stable complex with the polysaccharide which renders the polysaccharide bioavailable in the host system to produce the desired immunostimulative response.
  • the phospholipid component of the polysaccharide-phospholipid conjugate may be rendered into conjugatable form by reaction with suitable reagent(s), e.g., an appropriate bifunctional reagent.
  • suitable reagent(s) e.g., an appropriate bifunctional reagent.
  • only the polysaccharide may be modified to render it in conjugatable form, and in still other instances, both the polysaccharide and the phospholipid starting materials are modified to render them conjugatable, viz-a-vis one another.
  • the bifunctional reagent is N-succinimidyl-3-(2- pyridyldithio)-propionate, sometimes hereinafter referred to as SPDP
  • the phospholipid is dipalmatoylphosphatidyl-ethanolamine, sometimes hereinafter referred to DPPE.
  • SPDP and DPPE may be reacted with one another in a suitable solvent medium, e.g., chloroform.
  • the chloroform in the reaction volume advantageously is replaced, via evaporation of the chloroform under nitrogen atmosphere, with a suitable water-miscible solvent such as acetonitrile, to yield the DPPE-SPDP conjugate as the phospholipid component for subsequent reaction with the modified polysaccharide.
  • a suitable water-miscible solvent such as acetonitrile
  • the modified polysaccharide having for example a thiol functionality (as a result of reaction with a mercaptohydrazide compound) then is reacted with the SPDP-derivatized phospholipid to form a polysaccharide-phospholipid conjugate as the adjuvant product.
  • phospholipid compounds which may be advantageously employed includes fatty acid phosphatidylethanolamine compounds, whose fatty acid component includes two fatty acid moieties each of which is independently selected from the group consisting of lauroyl, palmatoyl, myristyl, oleyl, and stearyl, which in the subsequent discussion are designated by the letters L, P, M, O, and S, respectively, and in which the phosphatidylethanolamine moiety is designated PE.
  • illustrative phospholipid species based on the above ⁇ mentioned fatty acid functional groups which may be potentially usefully employed in the practice of the present invention include those identified in Table I below. Table I
  • an adjuvant which is administerable to a host animal by any of a variety of administration routes to provide a slow and controlled enhancement of immunological response.
  • the resulting adjuvant may be then be compounded for formulation purposes with any suitable antigens, carriers, excipients, stabilizers, additives, etc. and the formulation may be processed as necessary for end use or administration purposes.
  • the adjuvant formulation may be lyophilized to form a powder formulation which is amenable to administration by nebulization to a pulmonary locus of a host animal.
  • the fomulation may be subjected to sonication or other shear treatment, to yield a microparticle composition for convenient administration.
  • suitable antigen or antigens may be coordinately linked to the phospholipid and /or the polysaccharide moieties of the adjuvant, to provide an integrated vaccine formulation for effecting enhanced immunostimulative response from the host animal.
  • the host animals to which the adjuvant and adjuvant-containing vaccine formulations of the present invention are usefully administered include human as well as non-human mammals, fish, reptiles, etc.
  • compositions of the adjuvant of the present invention it may be useful in some applications to employ an antigen covalently linked to a phospholipid and /or polysaccharide moiety of the polysaccharide- phospholipid conjugate.
  • an antigen may be employed in mixture with the adjuvant of the invention.
  • the specific formulation of therapeutically effective compositions of the present invention may thus be carried out in any suitable manner which will render the adjuvant bioavailable, safe and effective in the subject to whom the formulation is administered.
  • the invention broadly contemplates therapeutic adjuvant formulations, which may for example comprise (i) at least one therapeutically effective antigen or vaccine; and (ii) at least one polysaccharide-phospholipid conjugate according to the invention.
  • Such therapeutic composition may for example comprise at least one antigenic agent selected from the group consisting of: (A) viruses, bacteria, mycoplasmas, fungi, and protozoa;
  • the therapeutic composition may therefore utilize any suitable antigen or vaccine component in combination with the polysaccharide-phospholipid conjugate of the invention, e.g., an antigenic agent selected from the group consisting of antigens from pathogenic and non-pathogenic organisms, viruses, and fungi, in combination with a polysaccharide-phospholipid conjugate.
  • an antigenic agent selected from the group consisting of antigens from pathogenic and non-pathogenic organisms, viruses, and fungi, in combination with a polysaccharide-phospholipid conjugate.
  • such therapeutic composition may suitably comprise proteins, peptides, antigens and vaccines which are pharmacologically active for disease states and conditions such as smallpox, yellow fever, distemper, cholera, fowl pox, scarlet fever, diphtheria, tetanus, whooping cough, influenza, rabies, mumps, measles, foot and mouth disease, and poliomyelitis.
  • the antigen and adjuvant are each present in an amount effective to elicit an immune response when the formulation is administered to a host animal, embryo, or ovum vaccinated therewith.
  • the resulting vaccine formulations including (i) an antigen, and (ii) the polysaccharide-phospholipid conjugate, are usefully employed to induce an immunological response in an animal, by administering to such animal the vaccine formulation, in an amount sufficient to produce an antibody response in such animal.
  • the modes of administration may comprise the use of any suitable means and /or methods for delivering the adjuvant or adjuvant-containing vaccine to a corporeal locus of the host animal where the adjuvant and associated antigens are immumostimulatively effective.
  • Delivery modes may include, without limitation, parenteral administration methods, such as subcutaneous (SC) injection, intravenous (IV) injection, nasal, ophthalmic, transdermal, intramuscular (IM), intradermal (ID), intraperitoneal (IP), intravaginal, pulmonary, and rectal administration, as well as non-parenteral, e.g., oral, administration.
  • the dose rate and suitable dosage forms for the adjuvant and vaccine compositions of the present invention may be readily determined by those of ordinary skill in the art without undue experimentation, by use of conventional antibody titer determination techniques and conventional bioefficacy/ biocompatibility protocols, and depending on the particular antigen or therapeutic agent employed with the adjuvant, the desired therapeutic effect, and the desired time span of bioactivity.
  • the adjuvant of the present invention may be usefully administered to the host animal with any other suitable pharmacologically or physiologically active agents, e.g., antigenic and /or other biologically active substances.
  • suitable pharmacologically or physiologically active agents e.g., antigenic and /or other biologically active substances.
  • a ⁇ -glucan-phospholipid conjugate in accordance with the present invention was formulated by the synthesis procedure described below.
  • ⁇ -Glucan was treated with sodium periodate to induce aldehyde formation in the polysaccharide.
  • 50 ⁇ l (5 ⁇ mole) of 0.1 M sodium periodate was added to a suspension of ⁇ -glucan (20 mg) in lml of water. The reaction took place over 15 hours at room temperature.
  • AMBH 2-acetamido-4-mercaptobutyric acid hydrazide
  • Dipalmitoylphosphatidylethanolamine (173 mg; 250 ⁇ moles) was dissolved in chloroform. Triethylamine (20 ⁇ l) and N-succinimidyl-3-(2- pyridylthio)-propionate (78 mg; 250 ⁇ moles) (Pierce Chemical Company (Rockford, IL)), denoted hereinafter as SPDP, were added in order. The mixture was gently stirred for 24 hours at room temperature. The chloroform was evaporated using nitrogen. The residue was dissolved in 4 ml of acetonitrile to provide a solution containing 62 ⁇ moles of SPDP conjugated dipalmitoylphosphatidylethanolamine per ml of solution.
  • a microdroplet emulsion formulation of the BSA was performed in accordance with the teachings of the aforementioned Haynes U.S. Patent 5,246,707. This vaccine formulation was used for comparison purposes.
  • the adjuvant properties of an adjuvant composition of the present invention were evaluated in vaccine formulations containing bovine serum albumin (BSA) antigen.
  • BSA bovine serum albumin
  • BSA bovine serum albumin
  • BSA-MD microdroplet emulsion form
  • FCA BSA with Freund's Complete Adjuvant
  • CF-1 mice (Charles River) approximately 25 grams in weight were used.
  • the wells of 96-well microtiter plates were coated with a 0.5 mg/ml solution of BSA used as antigen (50 ⁇ l aliquots added to the wells and allowed to dry in the freezer).
  • the wells were washed 3 times with wash buffer (Tris 20 mM, NaCl 0.8 M, 0.05% Tween-20, pH 7.4) followed by filling each well with an aqueous solution containing 1 mg/ml gelatin for 30 minutes followed by three washes with the wash buffer.
  • Serial dilutions of the sera 100 ⁇ l were added to the wells and kept overnight at 4°C.
  • the wells were washed 3 times with wash buffer and 100 ⁇ l of a 1:10,000 dilution of goat anti-mouse IgG- alkaline phosphatase conjugate (Organon Teknika Corporation, Charlotte, NC) was added. After 1 hour at room temperature, the wells were washed three times with wash buffer. Freshly prepared solution (200 ⁇ l) of the substrate (p-nitrophenyl phosphate disodium) 1 mg/ml in diethanolamine buffer pH 9.8 (97 ml diethanolamine, 0.2 g NaN 3 , 100 mg MgCl 2 - ⁇ H j O in 1 liter water) was added and kept at room temperature in the absence of light for 2 hours. The color development was quantitated at 405 nanometers in a microtiter plate reader.
  • the results are given in Figure 1.
  • the non-formulated antigen designated as BSA (as expected) had the minimum antibody titer while the microdroplet-formulated antigen (BSA-MD) provides better response than the non-formulated antigen at various dilutions of the anti-sera.
  • BSA-GL BSA-glucan formulation
  • BSA-MD BSA-microdroplet formulation
  • the adjuvant properties of an adjuvant composition of the present invention were evaluated in vaccine formulations containing bovine serum albumin (BSA) antigen.
  • BSA bovine serum albumin
  • BSA BSA
  • BSA-MD BSA-containing adjuvant formulation representative of the present invention, prepared by the procedure of Example II
  • BSA-Freund's BSA with Freund's Complete Adjuvant
  • mice Six week old BALB/c (Charles River) mice were injected s.c. with 200 ⁇ l of the adjuvant preparations. Each of the administered adjuvants preparations contained 200 ⁇ g/ml of BSA. In addition, a normal saline solution of BSA was prepared at 200 ⁇ g/ml. On day 14, each animal was boosted with freshly prepared adjuvant preparations, made with the same concentrations as the initial preparations. As in normal practice for boosting, FIA was used instead of FCA. At 1 month, 2 months or 3 months post-initial treatment, the mice were bled.
  • the wells of 96-well microtiter plates were coated with a 1 mg/mL solution of BSA (50 ⁇ L aliquots added to the wells and overnight at 4 ' C). The wells were washed three times with PBS/1% Tween 20. lOO ⁇ L of PBS containing 10%. goat serum and 1% Tween 20 were then incubated in the wells for 1 hour at 37 °C; plates were then washed 3 times with PBS/1% Tween 20.
  • Test sera were serially diluted (1:32 to 1:4096) in lOO ⁇ L of PBS/ 10% goat serum/ 1% Tween 20 and added to each well and incubated overnight at 4 ° C or for 2 hours at 37 " C; the plates were then washed 6 times with PBS/1% Tween 20.
  • Goat anti-mouse IgG or IgM coupled HRP antibody were diluted 1:1000 in PBS/1% Tween 20 and incubated in the plates for 2 hours at 37 " C; plates were then washed 6 times with PBS/1% Tween 20.
  • ABTS Peroxidase Substrate and Peroxidase Solution were used for development of the peroxidase reaction.
  • ELISA readings were performed with a Fisher Biotech, Microkinetics BT 2000 plates reader (405 nm wavelength).
  • FIG. 2 is a graph of IgG titer in mice plasma at 1:4096 dilution, at one, two and three months after injection, for vaccination with the respective BSA, BSA-MD, and BSA-Freund's compositions.
  • the non-formulated antigen (BSA) had the minimum titer
  • the microdroplet-formulated antigen (BSA-MD) representative of the present invention provided better response than Freund's Adjuvant with BSA (BSA-Freund's) through 3 months post-initial treatment.
  • the adjuvant compositions of the present invention are usefully employed with an antigen or an antigen-based vaccine, for administration to a host animal, embryo or ovum, to enhance immunostimulative response of the recipient host.
  • Such therapeutic compositions may for example comprise one or more antigenic agents such as (A) viruses, bacteria, mycoplasmas, fungi, and protozoa; (B) fragments, extracts, subunits, metabolites and recombinant constructs of (A); (C) fragments, subunits, metabolites and recombinant constructs of mammalian proteins and giycoproteins; and (D) tumor-specific antigens, and such therapeutic compositions may be pharmacologically active for disease states and conditions such as smallpox, yellow fever, distemper, cholera, fowl pox, scarlet fever, diphtheria, tetanus, whooping cough, influenza, rabies, mumps, measles, foot and mouth disease, and poliomyelitis, wherein the antigen and adjuvant are each present in an amount effective to elicit an immunological response when the formulation is administered to the host animal, embryo, or ovum vaccinated therewith.

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Abstract

An adjuvant for administration to a host animal to enhance immune response, comprising a polysaccharide-phospholipid conjugate, whose polysaccharide moiety may for example comprise a modified glucan, chitosan, or alginate polysaccharide, conjugated to a phospholipid. The adjuvant may be suitably formulated with an antigen to provide a therapeutic vaccine for a variety of disease states and/or physiological conditions.

Description

NOVEL ADJUVANT COMPOSITIONS AND VACCINE FORMULATIONS
COMPRISING SAME
DESCRIPTION
Field Of The Invention
The present invention relates to adjuvant compositions and to vaccine formulations comprising same, as well as to methods of making and using such adjuvants and vaccines.
Description of the Related Art
The injection of an antigen into an animal has long been regarded as an effective method of producing antisera (i.e., serum antibody) or increasing the antisera levels in the animal either for the protection of the host animal (i.e., vaccination) or to produce antisera for isolation and use in other animals.
Vaccines occupy a unique place in health care because unlike most therapies they are given to healthy people to prevent diseases. Because vaccination use has been a primary factor in controlling many childhood diseases, great effort is applied in expanding the use of vaccines. Vaccines are being developed for many diseases including cholera, malaria, herpes, chicken pox, and pneumonia.
It also has long been known that use of certain adjuvants can increase the titer of antisera produced against a foreign antigen and provide prolonged protection against the unwanted effect of the antigen itself or the pathogens carrying such antigen. The adjuvant will influence the titer, duration, isotype, and avidity of the antibody as well as influence cell-mediated immunity. Research and development efforts have focused on developing vaccine adjuvants that enhance the body's immunological response to vaccines with extended duration of effectiveness. Effective adjuvant formulations in the form of Freund's complete adjuvant (FCA) or Freund's incomplete adjuvant (FIA) have been known since the mid-1930's and have been used to improve the production of antisera against heterologous antigens in laboratory animals.
The key characteristics of Freund's complete adjuvant and Freund's incomplete adjuvant are the emulsification of the antigen in mineral oil to ensure the formation of a slow-release depot of the antigen at the injection at the injection site. Freund's complete adjuvant contains killed mycobacteria and apparently acts by preferentially inducing antibody against epitopes on denatured proteins. The result is higher levels of antisera produced when compared with antigen alone. However, Freund's complete and incomplete adjuvant are known to produce significant toxic complications.
In addition to causing chronic pain and suffering as an undesirable side effect, FCA induces local granulomas and possibly malignancies. For these reasons, FCA has never been approved for use in human or veterinary vaccines in the United States.
A major goal in the area of vaccine development is the production of vaccine formulations which include the efficacy and exclude the deleterious side effects of adjuvants such as FCA. Attempts to reduce the toxicity while retaining efficacy of adjuvants such as Freund's adjuvant have largely failed, in part, from a lack of understanding of the specific biological mechanism(s) responsible for adjuvant efficacy.
Several commercial adjuvant products are available which, while safer than Freund's adjuvant, have significantly lower effectiveness than Freund's adjuvant. For example, oil and water emulsions with the antigen adsorbed to the oil phase having brief retention are commercially available. Also commercially used is aluminum hydroxide where the antigen is adsorbed directly on the aluminum hydroxide. In another commercial product for experimental non-human use, available under the trademark Adjuvax, the antigen is physically incorporated in a polysaccharide matrix of a glucan polysaccharide.
The original adjuvants were substances of biological origin that enhanced a specific antibody response. Since mycobacterium has been effectively used as an adjuvant, attempts have been made to isolate those biologically active components from mycobacterium cell walls that are responsible for immunostimulation. The lipid fraction extracted from mycobacteria contains trehalose dimycolate (TDM) as an active component while the mycobacterium cell wall contains N-acetylmuramyl-L-alanine-D- isoglutamine, also known as muramyl dipeptide (MDP), as an active component. Lipopolysaccharides obtained from the cell wall of these gram- negative bacteria exhibit immunostimulating activity, but toxicity attributed to its lipid A portion has precluded its use. Glucan, a β-1, 3-polyglucose from Saccharomyces cerevisiae, a yeast, has been reported to induce antitumor effects, improve resistance to microbial pathogens and stimulate antibody response to a variety of antigens.
The toxicological issues associated with adjuvants of microbial origin have resulted in research on nonmicrobial substances. These nonmicrobial substances include detergents, salts, sugars, polyribonucleotides, and natural substances of mammalian origin. Both nonionic and cationic detergents have achieved success as adjuvants, with more lipophilic detergents being more effective. Saponins have amphipathic surface activity, so their mechanism for inducing adjuvant activity may be similar to that of detergents. Saponins are not used in human vaccines because of toxicological issues. Lymphokines and monokines have a very short biological half-life, so pharmacokinetic concerns preclude their use as adjuvants.
In the typical vaccine formulation comprising an antigen and a vehicle (carrier) component, a clear distinction between the vehicle and the adjuvant cannot always be made because many vehicles have adjuvant-like activity, which may result from immunostimulation effects and/or slow release of antigen. For example, aluminum salts are the most widely used vehicles in vaccines licensed for human and veterinary use. The antigen is believed to reside in the aluminum gel, releasing slowly over time to produce a continual challenge to the immune system. In addition to this clear vehicle effect, aluminum salts probably act as true adjuvants by virtue of their chemotactic properties for various immunological cells. Other examples of vehicles with adjuvant-like activities include water/oil emulsions, oil/water emulsions, microencapsulation, and liposomes.
For more than 20 years, the ability of liposomes to stimulate antibody response has been known, but issues in the development of appropriate components for liposomes as carriers for vaccines are still in debate. The antigen can be encapsulated into the aqueous spaces of the liposome core or attached to the external surface of the lipid bilayer. The adjuvant property of liposomes can be further enhanced by the inclusion of certain immunostimulants such as lipid A, lipopolysaccharide, or MDP. Liposomes are believed to exhibit their adjuvant properties by being taken up preferentially by macrophages, but liposomal delivery does not provide for a sustained release of antigen. Nanoparticles, solid colloidal particles from 10 to 1000 nm of synthetic polymers such as polymethylmethacrylate, are reported to be effective adjuvants whether the antigen is encapsulated within the nanoparticle or adsorbed to the nanoparticle surface. The adjuvant effect of these vaccines improves with increasing hydrophobicity and decreasing particle size. Because the polymethylmethacrylate nanoparticles are slowly biodegradable, the adjuvant effect may be caused by a continuing antigen challenge to the immune system.
A recent study evaluating different adjuvants for their ability to induce antibody in mice to HIV-2 split whole virus reported that polymethylmethacrylate nanoparticles was the best overall adjuvant when considering the immune response and observable toxic side-effects. However, the data also suggested that two or more different adjuvants may be necessary to induce the required immune response against physically different antigens. An alternative explanation of the study data is that the immunological response to each antigen is best augmented by a unique adjuvant. In either case, the development of alternative adjuvants is critical to the successful development of potent vaccine formulations.
The technical and patent literature describes various other attempts at improved adjuvants.
U.S. Patent 5,273,965 describes compounds of the saponin family which can be used to administer vaccines via nasal spray or eye drops.
Infection and Immunity, Sept. 1991, pp. 2978-2986, describes a poly(DL- lactide-co-glycolide) microsphere useful as an adjuvant for Staph. enterotoxin B toxoid. U.S. Patent 5, 057,503 to J.K. Czop, et al. discloses small molecular weight biologically active oligosaccharides which are interactive with β-glucan receptors on mammalian phageocytic cells. This unit ligand composition, a heptaglucoside, is described as being derivatizable with 2-aminopyridine to increase the capacity of the glucocide to stimulate β-glucan receptors and potentiate functions mediated by such receptors. The heptaglucoside is described as useful for vaccine or other immunomodulating agent preparations such as adjuvant therapy.
U.S. Patent 5, 189,028 to L.H. Nikl, et al. describes the stimulation of immune systems of fish by administration of a β-1, 3-glucan, particularly a β-1, 3-glucan having a β-1, 3-linked main chain with β-1, 6-linked single glucose side chains.
U.S. Patent 4,981,684 to N.M. MacKenzie, et al. discloses the formulation of adjuvant matrices comprising a water-insoluble antigen which is solubilized with a solubilizing agent, e.g., a detergent species, urea or guanidine, then admixed with a glycoside, a sterol, and optionally, a phospholipid, thereby forming an immuno-stimulating complex substantially without removal of the solubilizing agent.
U.S. Patent 5,032,401 to S. Jamas describes a pharmaceutical composition comprising whole glucan particles and a pharmacologically active substance such as a drug or antigen contained within, uniformly dispersed with, or chemically linked to the whole glucan particles.
U.S. Patents 5,091,187 and 5,091,188 to D.H. Haynes disclose phospholipid-coated microcrystal or microparticle compositions providing an injectible delivery form for administration of water-insoluble drugs to a mammalian host for sustained release. The pharmaceutically effective agent is produced in solid form coated with a membrane-forming lipid which stabilizes the active ingredient material by hydrophobic and hydrophilic interactions. The active solid ingredient-containing particles are formed in small finely divided form, by sonication or other process inducing high shear. U.S. Patent 5,246,707 discloses the sustained release delivery of water-soluble biomolecules and drugs using phospholipid-coated microcrystals, microdroplets and high-concentration liposomes. The phospholipid-coated microcrystal and the phospholipid-coated microdroplet are described as useable as vaccine adjuvants.
It therefore is an object of the present invention to provide an improved adjuvant having an immunostimulating character, but without toxic side effects.
It is another object of the present invention to provide a vaccine composition comprising such adjuvant which is safe and effective in use.
Other objects and advantages of the invention will be more fully apparent from the ensuing disclosure and appended claims.
SUMMARY OF THE INVENTION
The present invention relates to an adjuvant composition, which may be usefully employed with an antigen or an antigen-based vaccine, to enhance immunostimulative response. In a broad composition aspect, the invention relates to an adjuvant comprising a polysaccharide-phospholipid conjugate.
Particularly preferred polysaccharides of the adjuvant of the invention include β-glucan, chitosan, galactomanans, and alginates.
In another aspect, the present invention relates to a method of synthesizing an adjuvant from a polysaccharide.
The adjuvant may be synthesized from a polysaccharide and phospholipid, using any suitable reagents, including bifunctional or other polyfunctional reagents.
The adjuvant may for example be synthesized by the steps of:
reacting the polysaccharide with an oxidizing agent to form aldehyde functionality on the polysaccharide;
reacting the aldehyde-functionalized polysaccharide with an appropriate bifunctional reagent, to yield a polysaccharide function¬ alized with a linking functionality which is reactive with a phospholipid to further yield a polysaccharide-phospholipid conjugate; and
reacting the functionalized polysaccharide with a phospholipid to yield the polysaccharide-phospholipid conjugate. The invention in a further aspect comprises a vaccine composition including the adjuvant of the invention and an antigen for producing antibodies in an animal.
Further, the invention relates to inducing an immunological response in an animal comprising administering the vaccine including the adjuvant in an amount sufficient to produce an antibody response in such animal.
Other aspects and features of the invention will be more fully apparent from the ensuing disclosure and appended claims.
BRIEF DESCRIPTION OF THE DRAWINGS
FIGURE 1 is a graph of the antibody titer in mice, for vaccination with bovine serum albumin (BSA), BSA in microdroplet form (BSA-MD), BSA in microdroplet form with a β-glucan conjugate adjuvant according to one embodiment of the present invention (BSA-GMD), and BSA with Freund's
Complete Adjuvant (FCA).
FIGURE 2 is a graph of IgG titer in mice plasma at 1:4096 dilution, at one, two and three months after injection, for vaccination with bovine serum albumin (BSA), BSA in microdroplet form with a β-glucan conjugate adjuvant according to one embodiment of the present invention (BSA-MD), and BSA with Freund's Complete Adjuvant (BSA-Freund's).
DETAILED DESCRIPTION OF THE INVENTION, AND PREFERRED MODES
OF CARRYING OUT SAME
The present invention is based on the surprising and unexpected discovery that polysaccharides when conjugated with phospholipids form adjuvants which (i) increase the titer, duration, isotype and avidity of the antibody produced in a host animal, and (ii) have low toxicity and good effectiveness and safety characteristics in the host animal when compared to Freund's adjuvant.
The polysaccharides which are used to form the adjuvants of the invention may comprise any suitable polysaccharides, e.g., a polysaccharide having an immunostimulative activity.
Polysaccharides which may be used in adjuvant compositions in the broad practice of the present invention include species described in "Carbohydrate Chemistry," ed. by John F. Kennedy, Clarendon Press, Oxford, 1988; "The Carbohydrates, Chemistry and Biochemistry," ed. by W. Pigman and D. Horton, Academic Press, Inc., 1970; and "Chitin, Chitosan, and Related Enzymes," ed. by John P. Zikakis, Academic Press, Inc., 1984. Particularly preferred polysaccharide species include β-glucans, chitosan, galactomanans, and alginates, with β-glucans being currently most preferred.
As discussed hereinabove, the adjuvants of the present invention may be synthesized from a polysaccharide and phospholipid, via any suitable synthetic method, and using any suitable reagents, including bifunctional or other polyfunctional reagents.
Most generally, the polysaccharide is complexed by conjugation with a phospholipid by reaction, with may comprise oxidation of the polysaccharide, or other functionalizing reaction, to produce a functionalized polysaccharide which is of a form that is conjugatable with a phospholipid. The adjuvant may for example be synthesized by reacting the polysaccharide with an oxidizing agent to form aldehyde functionality on the polysaccharide, following which the aldehyde-functionalized polysaccharide is reacted with an appropriate bifunctional reagent, to yield a polysaccharide functionalized with a linking functionality. The linking functionality is reactive with a selected phospholipid to further yield a polysaccharide- phospholipid conjugate.
In one specific synthesis method within the scope of the broad invention, the bifunctional reagent in the above-described synthesis method comprises a thiol hydrazide compound, which is employed in the synthesis procedure to yield a thiol-functionalized polysaccharide. The thiol- functionalized polysaccharide subsequently is reacted with a phospholipid, to yield a polysaccharide-phospholipid conjugate as the aforementioned adjuvant.
As a further specific example of the synthesis of an adjuvant in accordance with the present invention, the starting polysaccharide is reacted with an oxidizing reagent such as a periodate compound, to convert oxidizable functional groups of the polysaccharide to corresponding aldehyde functionality (-CHO pendant groups). The resulting aldehyde-functionalized polysaccharide then is reacted with a mercaptohydrazide compound, such as for example 2-acetamido-4-mercapto-butyric acid hydrazide (AMBH), or other suitable bifunctional reagent, to provide a suitable reactive moiety (end group) on the functionalized polysaccharide for linking of a phospholipid conjugate thereto.
In the synthesis of the adjuvant conjugates of the present invention, reagents other than the bifunctional reagents described in the preceding paragraph may be advantageously employed, including for example reagents which may not require initial oxidation of the polysaccharide. A phospholipid may be conjugated to an existing functional group on the polysaccharide, such as an amino or a hydroxyl function, by methods known in the art of synthetic chemistry.
The phospholipid conjugate which is used to form the polysaccharide- phospholipid adjuvant of the present invention may comprise any suitable phospholipid, which is coordinatable, e.g., by covalent, ionic, hydrogen, associative, or other conjugative bonding, to form a pharmacologically stable complex with the polysaccharide which renders the polysaccharide bioavailable in the host system to produce the desired immunostimulative response.
Within the broad practice of the present invention, the phospholipid component of the polysaccharide-phospholipid conjugate may be rendered into conjugatable form by reaction with suitable reagent(s), e.g., an appropriate bifunctional reagent. In some instances of the practice of the present invention, it may be advantageous to functionalize a phospholipid so that it is directly conjugatable with the polysaccharide, and so that no intermediate reaction(s) involving the polysaccharide are necessary prior to conjugating the polysaccharide with a phospholipid. In other instances, only the polysaccharide may be modified to render it in conjugatable form, and in still other instances, both the polysaccharide and the phospholipid starting materials are modified to render them conjugatable, viz-a-vis one another.
As one specific example of rendering a phospholipid component in suitable form for conjugation with a polysaccharide, by reacting a bifunctional reagent with a phospholipid, the bifunctional reagent is N-succinimidyl-3-(2- pyridyldithio)-propionate, sometimes hereinafter referred to as SPDP, and the phospholipid is dipalmatoylphosphatidyl-ethanolamine, sometimes hereinafter referred to DPPE. SPDP and DPPE may be reacted with one another in a suitable solvent medium, e.g., chloroform. The chloroform in the reaction volume advantageously is replaced, via evaporation of the chloroform under nitrogen atmosphere, with a suitable water-miscible solvent such as acetonitrile, to yield the DPPE-SPDP conjugate as the phospholipid component for subsequent reaction with the modified polysaccharide. The modified polysaccharide, having for example a thiol functionality (as a result of reaction with a mercaptohydrazide compound) then is reacted with the SPDP-derivatized phospholipid to form a polysaccharide-phospholipid conjugate as the adjuvant product.
While any suitable phospholipid constituent may be employed in the broad practice of the invention, one particular class of phospholipid compounds which may be advantageously employed includes fatty acid phosphatidylethanolamine compounds, whose fatty acid component includes two fatty acid moieties each of which is independently selected from the group consisting of lauroyl, palmatoyl, myristyl, oleyl, and stearyl, which in the subsequent discussion are designated by the letters L, P, M, O, and S, respectively, and in which the phosphatidylethanolamine moiety is designated PE. Thus, illustrative phospholipid species based on the above¬ mentioned fatty acid functional groups, which may be potentially usefully employed in the practice of the present invention include those identified in Table I below. Table I
Compound Desi nation dipalmitoylphosphatidylethanolamine DPPE dilauroylphosphatidylethanolamine DLPE dimyristoylphosphatidylethanolamine DMPE dioleylphosphatidylethanolamine DOPE distearylphosphatidylethanolamine DSPE lauroylpalmitoylphosphatidylethanolamine LPPE lauroylmyristoylphosphatidylethanolamine LMPE lauroyloleylphosphatidylethanolamine LOPE lauroylstearylphosphatidylethanolamine LSPE palmitoylmyristoylphosphatidylethanolamine PMPE palmitoyloleylphosphatidylethanolamine POPE palmitoylstearylphosphatidylethanolamine PSPE oleylstearylphosphatidylethanolamine OSPE oleylmyristoylphosphatidylethanolamine OMPE myristoylstearylphosphatidylethanolamine MSPE
By conjugation of the phospholipid to the polysaccharide, there is formed an adjuvant which is administerable to a host animal by any of a variety of administration routes to provide a slow and controlled enhancement of immunological response.
The resulting adjuvant may be then be compounded for formulation purposes with any suitable antigens, carriers, excipients, stabilizers, additives, etc. and the formulation may be processed as necessary for end use or administration purposes. For example, the adjuvant formulation may be lyophilized to form a powder formulation which is amenable to administration by nebulization to a pulmonary locus of a host animal. Alternatively, the fomulation may be subjected to sonication or other shear treatment, to yield a microparticle composition for convenient administration. As a further variation of the compositions of the present invention, suitable antigen or antigens may be coordinately linked to the phospholipid and /or the polysaccharide moieties of the adjuvant, to provide an integrated vaccine formulation for effecting enhanced immunostimulative response from the host animal.
The host animals to which the adjuvant and adjuvant-containing vaccine formulations of the present invention are usefully administered include human as well as non-human mammals, fish, reptiles, etc.
In formulations of the adjuvant of the present invention, it may be useful in some applications to employ an antigen covalently linked to a phospholipid and /or polysaccharide moiety of the polysaccharide- phospholipid conjugate. Alternatively, an antigen may be employed in mixture with the adjuvant of the invention. The specific formulation of therapeutically effective compositions of the present invention may thus be carried out in any suitable manner which will render the adjuvant bioavailable, safe and effective in the subject to whom the formulation is administered.
The invention broadly contemplates therapeutic adjuvant formulations, which may for example comprise (i) at least one therapeutically effective antigen or vaccine; and (ii) at least one polysaccharide-phospholipid conjugate according to the invention.
Such therapeutic composition may for example comprise at least one antigenic agent selected from the group consisting of: (A) viruses, bacteria, mycoplasmas, fungi, and protozoa;
(B) fragments, extracts, subunits, metabolites and recombinant constructs of (A);
(C) fragments, subunits, metabolites and recombinant constructs of mammalian proteins and giycoproteins; and
(D) tumor-specific antigens.
The therapeutic composition may therefore utilize any suitable antigen or vaccine component in combination with the polysaccharide-phospholipid conjugate of the invention, e.g., an antigenic agent selected from the group consisting of antigens from pathogenic and non-pathogenic organisms, viruses, and fungi, in combination with a polysaccharide-phospholipid conjugate.
As a futher example, such therapeutic composition may suitably comprise proteins, peptides, antigens and vaccines which are pharmacologically active for disease states and conditions such as smallpox, yellow fever, distemper, cholera, fowl pox, scarlet fever, diphtheria, tetanus, whooping cough, influenza, rabies, mumps, measles, foot and mouth disease, and poliomyelitis. In the resulting vaccine formulation, comprising (i) an antigen, and (ii) the polysaccharide-phospholipid conjugate, the antigen and adjuvant are each present in an amount effective to elicit an immune response when the formulation is administered to a host animal, embryo, or ovum vaccinated therewith. The resulting vaccine formulations, including (i) an antigen, and (ii) the polysaccharide-phospholipid conjugate, are usefully employed to induce an immunological response in an animal, by administering to such animal the vaccine formulation, in an amount sufficient to produce an antibody response in such animal.
The modes of administration may comprise the use of any suitable means and /or methods for delivering the adjuvant or adjuvant-containing vaccine to a corporeal locus of the host animal where the adjuvant and associated antigens are immumostimulatively effective. Delivery modes may include, without limitation, parenteral administration methods, such as subcutaneous (SC) injection, intravenous (IV) injection, nasal, ophthalmic, transdermal, intramuscular (IM), intradermal (ID), intraperitoneal (IP), intravaginal, pulmonary, and rectal administration, as well as non-parenteral, e.g., oral, administration.
The dose rate and suitable dosage forms for the adjuvant and vaccine compositions of the present invention may be readily determined by those of ordinary skill in the art without undue experimentation, by use of conventional antibody titer determination techniques and conventional bioefficacy/ biocompatibility protocols, and depending on the particular antigen or therapeutic agent employed with the adjuvant, the desired therapeutic effect, and the desired time span of bioactivity.
The adjuvant of the present invention may be usefully administered to the host animal with any other suitable pharmacologically or physiologically active agents, e.g., antigenic and /or other biologically active substances. The features and advantages of the invention will be more fully illlustrated by the following non-limiting examples, wherein all parts and percentages are by weight, unless otherwise expressly stated.
Example I
A β-glucan-phospholipid conjugate in accordance with the present invention was formulated by the synthesis procedure described below.
Modification of β-Glucan β-Glucan was conjugated to AMBH as follows.
β-Glucan was treated with sodium periodate to induce aldehyde formation in the polysaccharide. 50 μl (5μmole) of 0.1 M sodium periodate was added to a suspension of β-glucan (20 mg) in lml of water. The reaction took place over 15 hours at room temperature. To the resulting suspension was added 50 μl (5 μmole) of 0.1 M 2-acetamido-4-mercaptobutyric acid hydrazide dissolved in acetonitrile (AMBH, Molecular Probes, Inc.). After stirring at room temperature for 24 hours, the AMBH conjugated to β-glucan suspension was used as such for conjugation with SPDP derivatized phospholipid.
Synthesis of SPDP Conjugated Dipalmitoylphosphatidylethanolamine
Dipalmitoylphosphatidylethanolamine (173 mg; 250 μmoles) was dissolved in chloroform. Triethylamine (20 μl) and N-succinimidyl-3-(2- pyridylthio)-propionate (78 mg; 250 μmoles) (Pierce Chemical Company (Rockford, IL)), denoted hereinafter as SPDP, were added in order. The mixture was gently stirred for 24 hours at room temperature. The chloroform was evaporated using nitrogen. The residue was dissolved in 4 ml of acetonitrile to provide a solution containing 62 μmoles of SPDP conjugated dipalmitoylphosphatidylethanolamine per ml of solution.
Preparation of β-Glucan-Phospholipid Conjugate SPDP conjugated dipalmitoylphosphatidylethanolamine (100 μl containing 6.2 μmoles) was added to 1 ml of the suspension of AMBH- conjugated β-glucan. The mixture was stirred gently for 24 hours at room temperature to yield the conjugate product.
Example II
β-Glucan-Phospholipid Conjugate BSA Formulation (BSA-GL1
A mixture of 0.5 ml of the β-glucan-phospholipid conjugate product suspension of Example I and 0.2 ml of aqueous BSA (1 mg/ml), 140 mg of egg phosphatidylcholine, 70 mg of vitamin E, 70 mg of Squalene, and 0.75 ml of phosphate buffered saline, was sonicated with a probe sonicator for 15 minutes at 4 degrees Centrigrade, to form the β-glucan-phospholipid conjugate-BSA vaccine emulsion formulation.
Example III
Microdroplet Adjuvant Formulation of BSA (BSA-MD)
A microdroplet emulsion formulation of the BSA was performed in accordance with the teachings of the aforementioned Haynes U.S. Patent 5,246,707. This vaccine formulation was used for comparison purposes.
A mixture of 0.2 ml of aqueous BSA (lmg/ml), 140 mg of egg phosphatidylcholine, 70 mg of vitamin E, 70 mg of squalene, and 1.25 ml of phosphate buffered saline, was sonicated with a probe sonicator for 15 minutes at 4 degrees Centrigrade, to form the BSA-MD microdroplet vaccine formulation.
Example IV
Adjuvant Studies in Mice
The adjuvant properties of an adjuvant composition of the present invention were evaluated in vaccine formulations containing bovine serum albumin (BSA) antigen. The comparative studies were carried out in mice, and the results included a determination of the antibody titer produced by vaccination with the respective vaccine formulations.
The tests included vaccination of respective test animals with the following vaccine formulations: (i) bovine serum albumin (BSA) in saline, (ii) BSA in microdroplet emulsion form (BSA-MD), formulated in accordance with Example III above, (iii) BSA with a β-glucan-phospholipid conjugate adjuvant according to one embodiment of the present invention, prepared by the procedure of Example II (BSA-GL), and (iv) BSA with Freund's Complete Adjuvant (FCA). The results, discussed hereinafter in greater detail, are shown in the graph of Figure 1.
Experimental Design
CF-1 mice (Charles River) approximately 25 grams in weight were used.
Mice (n=5 per group) were injected i.p. with 50 μl of each formulation containing the same amount of antigen on day 0 and given a booster injection of the same quantity as the original injection on day 14, and serum samples from each mouse were analyzed on day 28. Screening the Sera Samples
The wells of 96-well microtiter plates were coated with a 0.5 mg/ml solution of BSA used as antigen (50 μl aliquots added to the wells and allowed to dry in the freezer). The wells were washed 3 times with wash buffer (Tris 20 mM, NaCl 0.8 M, 0.05% Tween-20, pH 7.4) followed by filling each well with an aqueous solution containing 1 mg/ml gelatin for 30 minutes followed by three washes with the wash buffer. Serial dilutions of the sera (100 μl) were added to the wells and kept overnight at 4°C. The wells were washed 3 times with wash buffer and 100 μl of a 1:10,000 dilution of goat anti-mouse IgG- alkaline phosphatase conjugate (Organon Teknika Corporation, Charlotte, NC) was added. After 1 hour at room temperature, the wells were washed three times with wash buffer. Freshly prepared solution (200 μl) of the substrate (p-nitrophenyl phosphate disodium) 1 mg/ml in diethanolamine buffer pH 9.8 (97 ml diethanolamine, 0.2 g NaN3, 100 mg MgCl2 -όHjO in 1 liter water) was added and kept at room temperature in the absence of light for 2 hours. The color development was quantitated at 405 nanometers in a microtiter plate reader.
Results The results are given in Figure 1. The non-formulated antigen, designated as BSA (as expected) had the minimum antibody titer while the microdroplet-formulated antigen (BSA-MD) provides better response than the non-formulated antigen at various dilutions of the anti-sera. The immune response of the BSA-glucan formulation (BSA-GL), comprising a β-glucan- phospholipid conjugate according to the present invention, was found to be even better than the BSA-microdroplet formulation (BSA-MD) and as good as that of the antigen formulated in Freund's Complete Adjuvant at all dilutions of the anti-sera tested. Example V
In separate tests comparing adjuvant properties of (1) unmodified β-glucan (not conjugated with phospholipid) with (2) a modified β-glucan- phospholipid conjugate according to the present invention, the adjuvant- containing unmodified β-glucan did not provide a similar enhancement of antibody titer achieved by the modified β-glucan-phospholipid conjugate.
Example VI
The adjuvant properties of an adjuvant composition of the present invention were evaluated in vaccine formulations containing bovine serum albumin (BSA) antigen. The comparative studies were carried out in mice, and the results included a determination of the antibody titer produced by vaccination with the respective vaccine formulations.
The tests included vaccination of respective test animals with the following vaccine formulations: (i) BSA (ii) a BSA-containing adjuvant formulation representative of the present invention, prepared by the procedure of Example II (BSA-MD), and (iv) BSA with Freund's Complete Adjuvant (BSA-Freund's). The results, discussed below in greater detail, are shown in the graph of Figure 2.
Adjuvant Studies in Mice Six week old BALB/c (Charles River) mice were injected s.c. with 200μl of the adjuvant preparations. Each of the administered adjuvants preparations contained 200 μg/ml of BSA. In addition, a normal saline solution of BSA was prepared at 200 μg/ml. On day 14, each animal was boosted with freshly prepared adjuvant preparations, made with the same concentrations as the initial preparations. As in normal practice for boosting, FIA was used instead of FCA. At 1 month, 2 months or 3 months post-initial treatment, the mice were bled.
Screening the Sera Samples
The wells of 96-well microtiter plates were coated with a 1 mg/mL solution of BSA (50μL aliquots added to the wells and overnight at 4 ' C). The wells were washed three times with PBS/1% Tween 20. lOOμL of PBS containing 10%. goat serum and 1% Tween 20 were then incubated in the wells for 1 hour at 37 °C; plates were then washed 3 times with PBS/1% Tween 20. Test sera were serially diluted (1:32 to 1:4096) in lOOμL of PBS/ 10% goat serum/ 1% Tween 20 and added to each well and incubated overnight at 4 ° C or for 2 hours at 37 " C; the plates were then washed 6 times with PBS/1% Tween 20. Goat anti-mouse IgG or IgM coupled HRP antibody were diluted 1:1000 in PBS/1% Tween 20 and incubated in the plates for 2 hours at 37 " C; plates were then washed 6 times with PBS/1% Tween 20. ABTS Peroxidase Substrate and Peroxidase Solution were used for development of the peroxidase reaction. ELISA readings were performed with a Fisher Biotech, Microkinetics BT 2000 plates reader (405 nm wavelength).
Results
The results are given in Figure 2, which is a graph of IgG titer in mice plasma at 1:4096 dilution, at one, two and three months after injection, for vaccination with the respective BSA, BSA-MD, and BSA-Freund's compositions. As shown, the non-formulated antigen (BSA) had the minimum titer, while the microdroplet-formulated antigen (BSA-MD) representative of the present invention provided better response than Freund's Adjuvant with BSA (BSA-Freund's) through 3 months post-initial treatment.
INDUSTRIAL APPLICABILITY
The adjuvant compositions of the present invention are usefully employed with an antigen or an antigen-based vaccine, for administration to a host animal, embryo or ovum, to enhance immunostimulative response of the recipient host.
Such therapeutic compositions may for example comprise one or more antigenic agents such as (A) viruses, bacteria, mycoplasmas, fungi, and protozoa; (B) fragments, extracts, subunits, metabolites and recombinant constructs of (A); (C) fragments, subunits, metabolites and recombinant constructs of mammalian proteins and giycoproteins; and (D) tumor-specific antigens, and such therapeutic compositions may be pharmacologically active for disease states and conditions such as smallpox, yellow fever, distemper, cholera, fowl pox, scarlet fever, diphtheria, tetanus, whooping cough, influenza, rabies, mumps, measles, foot and mouth disease, and poliomyelitis, wherein the antigen and adjuvant are each present in an amount effective to elicit an immunological response when the formulation is administered to the host animal, embryo, or ovum vaccinated therewith.

Claims

THE CLAIMS
1. An adjuvant useful for administration to a host animal to stimulate immune response, comprising a polysaccharide-phospholipid conjugate.
2. An adjuvant according to claim 1, wherein the polysaccharide- phospholipid conjugate comprises a polysaccharide selected from the group consisting of glucans, chitosan, galactomanans, and alginates.
3. An adjuvant according to claim 1, wherein the polysaccharide- phospholipid conjugate comprises a glucan polysaccharide.
4. An adjuvant according to claim 1, wherein the polysaccharide- phospholipid conjugate comprises a β-glucan polysaccharide.
5. An adjuvant according to claim 1, wherein the polysaccharide- phospholipid conjugate includes a phospholipid moiety which comprises a fatty acid phosphatidylethanolamine moiety.
6. An adjuvant according to claim 5, wherein the fatty acid phosphatidylethanolamine is selected from the group consisting of:
dipalmitoylphosphatidylethanolamine, dilauroylphosphatidylethanolamine, dimyristoylphosphatidylethanolamine, dioleylphosphatidylethanolamine, distearylphosphatidylethanolamine, lauroylpalmitoylphosphatidylethanolamine, lauroylmyristoylphosphatidylethanolamine, lauroyloleylphosphatidylethanolamine, lauroylstearylphosphatidylethanolamine, palmitoylmyristoylphosphatidylethanolamine, palmitoyloleylphosphatidylethanolamine, palmitoylstearylphosphatidylethanolamine, oleylstearylphosphatidylethanolamine, oleylmyristoylphosphatidylethanolamine, and myristoylstearylphosphatidylethanolamine.
7. An adjuvant according to claim 1, wherein the polysaccharide- phospholipid conjugate is in a lyophilized form.
8. An adjuvant according to claim 1, wherein the polysaccharide- phospholipid conjugate is in a microparticle form.
9. An adjuvant according to claim 1, having an antigen covalently linked to a phospholipid and /or polysaccharide moiety of the polysaccharide- phospholipid conjugate.
10. A therapeutic composition, comprising:
(i) a therapeutically effective antigen or vaccine; and
(ii) a polysaccharide-phospholipid conjugate.
11. A therapeutic composition according to claim 10, wherein the antigen or vaccine comprises at least one antigenic agent selected from the group consisting of: (A) viruses, bacteria, mycoplasmas, fungi, and protozoa;
(B) fragments, extracts, subunits, metabolites and recombinant constructs of (A);
(C) fragments, subunits, metabolites, and recombinant constructs of mammalian proteins or giycoproteins,
(D) tumor-specific antigens; and
(E) combinations thereof.
12. A therapeutic composition according to claim 10, wherein the antigen or vaccine comprises an antigenic agent selected from the group consisting of antigens from pathogenic and non-pathogenic organisms, viruses, and fungi.
13. A therapeutic composition according to claim 10, wherein the antigen or vaccine comprises an antigen for a disease state selected from the group consisting of: smallpox, yellow fever, distemper, cholera, fowl pox, scarlet fever, diphtheria, tetanus, whooping cough, influenza, rabies, mumps, measles, foot and mouth disease, and poliomyelitis.
14. A vaccine formulation comprising (i) at least one antigen, and (ii) at least one adjuvant comprising a polysaccharide-phospholipid conjugate, wherein the antigen and adjuvant are each present in an amount effective to elicit an immune response when administered to a host animal vaccinated therewith.
15. A vaccine formulation according to claim 14, further comprising at least one component of the group consisting of: vitamin E, squalene, and lecithin.
16. A method of inducing an immunological response in an animal, embryo or ovum, comprising administering to said animal, embryo or ovum, an adjuvant comprising a polysaccharide-phospholipid conjugate, in an amount sufficient to produce an antibody response in said animal, embryo or ovum.
17. A method according to claim 16, comprising administration to an animal of said adjuvant in combination with at least one therapeutic component selected from the group consisting of: (i) other adjuvants, and (ii) antigens.
18. A method according to claim 16, comprising administration to an animal by an administration mode selected from the group consisting of subcutaneous injection, intravenous injection, oral, nasal, ophthalmic, transdermal, intramuscular, intradermal, intraperitoneal, intravaginal, pulmonary, and rectal administration.
19. A method according to claim 16, comprising administration to an animal by parenteral administration.
20. A method according to claim 16, comprising administration to said animal by oral administration.
21. A method of inducing an immunological response in an animal, embryo or ovum, comprising administering to said animal, embryo or ovum, a vaccine formulation including (i) at least one antigen, and (ii) an adjuvant comprising at least one polysaccharide-phospholipid conjugate, in an amount sufficient to produce an antibody response in said animal, embryo or ovum.
22. A method of synthesizing an adjuvant from an immunostimulatively effective polysaccharide, comprising conjugating the polysaccharide with a phospholipid to form a polysaccharide-phospholipid conjugate.
23. A method according to claim 22, wherein at least one of said polysaccharide and said phospholipid is reacted with a bifunctional reagent to yield a functionalized conjugate component which is reactive in the conjugation of the polysaccharide and the phospholipid to form the polysaccharide-phospholipid conjugate.
24. A method according to claim 23, wherein said phospholipid is reacted with a bifunctional reagent.
25. A method according to claim 24, wherein said bifunctional reagent is N-succinimidyl-3-(2-pyridyldithio)-propionate.
26. A method according to claim 23, wherein the polysaccharide- phospholipid conjugate includes a phospholipid moiety which comprises a fatty acid phosphatidylethanolamine.
27. A method according to claim 26, wherein the fatty acid phosphatidylethanolamine is selected from the group consisting of:
dipalmitoylphosphatidylethanolamine, dilauroylphosphatidylethanolamine, dimyristoylphosphatidylethanolamine, dioleylphosphatidylethanolamine, distearylphosphatidylethanolamine, lauroylpalmitoylphosphatidylethanolamine, lauroylmyristoylphosphatidylethanolamine, lauroyloleylphosphatidylethanolamine, lauroylstearylphosphatidylethanolamine, palmitoylmyristoylphosphatidylethanolamine, palmitoyloleylphosphatidylethanolamine, palmitoylstearylphosphatidylethanolamine, oleylstearylphosphatidylethanolamine, oleylmyristoylphosphatidylethanolamine, and myristoylstearylphosphatidylethanolamine.
28 A method according to claim 22, comprising the steps of:
reacting a polysaccharide with an oxidizing agent to form aldehyde functionality on the polysaccharide;
reacting the aldehyde-functionalized polysaccharide with a thiol hydrazide compound, to yield a thiol-functionalized polysaccharide;
reacting the thiol-functionalized polysaccharide with a phospholipid derivatized with a thio-reactive functional group comprising a phosphatidylethanolamine, to yield said polysaccharide-phospholipid conjugate as said adjuvant.
29. A method according to claim 22, further comprising compounding the adjuvant with at least one antigen to form a vaccine formulation.
30. A method according to claim 29, wherein the compounding of the adjuvant with an antigen comprises reactively complexing the antigen with the adjuvant so that the antigen is chemically bonded to the adjuvant.
31. A method according to claim 29, wherein the vaccine formulation is subjected to shear conditions to form a microparticle composition therefrom.
PCT/US1996/011051 1995-06-26 1996-06-26 Novel adjuvant compositions and vaccine formulations comprising same WO1997001330A1 (en)

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