WO1996001417A1 - Spectrophotometric equipment and use thereof - Google Patents

Spectrophotometric equipment and use thereof Download PDF

Info

Publication number
WO1996001417A1
WO1996001417A1 PCT/SE1995/000808 SE9500808W WO9601417A1 WO 1996001417 A1 WO1996001417 A1 WO 1996001417A1 SE 9500808 W SE9500808 W SE 9500808W WO 9601417 A1 WO9601417 A1 WO 9601417A1
Authority
WO
WIPO (PCT)
Prior art keywords
cuvette
light
equipment according
rear wall
spectrophotometric equipment
Prior art date
Application number
PCT/SE1995/000808
Other languages
French (fr)
Inventor
Lars Henrik Folke ANDRÉN
Original Assignee
Tecator Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tecator Ab filed Critical Tecator Ab
Priority to AU29413/95A priority Critical patent/AU2941395A/en
Publication of WO1996001417A1 publication Critical patent/WO1996001417A1/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/01—Arrangements or apparatus for facilitating the optical investigation
    • G01N21/03—Cuvette constructions
    • G01N21/0303—Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment

Definitions

  • the present invention refers to a spectrophotometric equipment comprising a light emitting source in front of a cuvette and a detector behind said cuvette.
  • the detector measures the absorp ⁇ tion, in a sample material charged in the cuvette, of the light from the light source.
  • the emitted light or a portion of the emitted light passes through a transparent front wall of the cuvette, the sample material and a transparent rear wall of the cuvette.
  • the invention furthermore relates to the use of said spectrophotometric equipment.
  • Emitted light from the light source can be a so called white light or a monochromatic light, emanating from a monochromator of suitable constructional design.
  • the detector can be a simple photodetector or consist of a dispersion part, such as a poly- chromator together with a number of detectors for certain wave ⁇ lengths.
  • An ordinary spectrophotometer setting is based on reflection, i.e. light reflected from an exposed sample in the cuvette of a spectrophotometer is measured.
  • Spectrophotometers based on transmission are also well-known. The amount of light passing through the sample is thereby mea ⁇ sured.
  • the present invention relates to such a spectrophoto ⁇ meter equipment.
  • Cuvettes used with transmission spectrophotometers are designed with a front and a rear wall forming a right angle in relation to the optical axis of the light emitted from the light source.
  • Cuvettes having such a design very often perform poorly with many products of interest for spectrophotometric analyses.”
  • the poor performance is normally related to the chemical and physi ⁇ cal nature and properties of the products, which cause large variations in the absorbency levels between different samples as well as between different measurements on the same sample. Varying structures of various samples imply similar problems due to varying density when charged in a cuvette. The samples can thus not be satisfactorily analysed using an equipment as herein disclosed.
  • the present invention refers to a spectrophotometric equipment comprising a light emitting source arranged in front of a cuvette and a detector arranged behind said cuvette, which detector measures the absorption of emitted light in a sample material introduced into the cuvette.
  • the emitted light or a portion of the said light passes, prior to recording in the detector, through a transparent front wall of the cuvette, the sample material and a transparent rear wall of the cuvette.
  • the equipment is characterised in that the front wall and/or the rear wall of the cuvette forms an oblique angle in relation to the optical axis of the emitted light.
  • the cuvette can thereby be wedge-shaped, cylindrical, spherical or the like giving above oblique angle in relation to said optical axis.
  • At least the front and rear wall of the cuvette are made of glass, quartz or other material transparent to the wavelengths of used light.
  • the front wall of the cuvette is, according to one preferred embodiment of the invention, designed to give an oblique angle in relation to the optical axis of the light emitted from the light source, while the rear wall forms a right angle in rela ⁇ tion of said optical axis.
  • the aforementioned embodiments of the cuvette according to the present invention give the major advantages of less variations in absorbency levels between different sample materials and types as well as a reduced difference between the highest and the lowest value of each spectra.
  • a single transmission spect ⁇ rophotometer can thus with said cuvette at a very high degree of reliability analyse a large number of different materials.
  • Used light source normally works within the wavelength area of 800-1100 nm, which is near the infrared light.
  • the light that passes through the cuvette has a comparatively narrow divergence when entering the sample but rapidly diverges through reflection and change of direction as soon as it has entered into the sample material.
  • 1/100 to 1/100000 of entered light passes through a finely powdered sample, which sample state is a preferred embodiment of the invention. It is often, in order to reproduce analytical results, necessary to prepare a sample material used in different analysis similarly or equally.
  • the design of the cuvette according to the present invention furthermore, facilitates filling and packing of powdered mate ⁇ rials thereinto.
  • the sample material can, of course, also be present as granules, a slurry, a paste, a liquid or the like.
  • the present invention also refers to the use of aforementioned spectrophotometric equipment for analyses of materials such as fodders and foodstuffs, whereby these products are available as granules, a powder, a slurry, a paste or a liquid or are trans ⁇ ferred into any of above states prior to the analysis.
  • the use is, of course, not limited to above materials and/or states.
  • the equipment according to the present invention can advanta ⁇ geously be used for analysis of all types of sample materials giving rise to discussed problems.
  • the equipment according to the present invention is especially suitable for analyses of materials containing fat and/or protein.
  • the spectrophotometric equipment comprises a monochromatic light source 1 arranged in front of a cuvette 2 and a detector unit 2 arranged behind said cuvette 2.
  • a finely powdered material 4, a fodder is charged in the cuvette 2.
  • Light emitted from the light source 1 will pass through a transparent front wall 5 of the cuvette 2, the fodder 4 and a transparent rear wall 6 of the cuvette 2 prior to measurement in the detector 3.
  • the front wall 5 forms an oblique angle in relation to the optical axis 7 of the light.

Abstract

Spectrophotometric equipment comprising a light source (1) arranged in front of a cuvette (2) and a detector (3) arranged behind said cuvette (2). The detector (3) measures absorption of light, emitted from the light source (1), in a sample material (4) charged in the cuvette (2), whereby the light or a portion of the light passes through a front wall (5) of the cuvette (2), the sample material (4) and a rear wall (6) of the cuvette prior to measurement in the detector (3). The equipment is characterised in that the front wall (5) and/or the rear wall (6) of the cuvette (2) forms an oblique angle in relation to the optical axis (7) of the emitted light.

Description

SPECTROPHOTOMETRIC EQUIPMENT AND USE THEREOF
The present invention refers to a spectrophotometric equipment comprising a light emitting source in front of a cuvette and a detector behind said cuvette. The detector measures the absorp¬ tion, in a sample material charged in the cuvette, of the light from the light source. The emitted light or a portion of the emitted light passes through a transparent front wall of the cuvette, the sample material and a transparent rear wall of the cuvette. The invention furthermore relates to the use of said spectrophotometric equipment.
Emitted light from the light source can be a so called white light or a monochromatic light, emanating from a monochromator of suitable constructional design. The detector can be a simple photodetector or consist of a dispersion part, such as a poly- chromator together with a number of detectors for certain wave¬ lengths.
An ordinary spectrophotometer setting is based on reflection, i.e. light reflected from an exposed sample in the cuvette of a spectrophotometer is measured.
Spectrophotometers based on transmission are also well-known. The amount of light passing through the sample is thereby mea¬ sured. The present invention relates to such a spectrophoto¬ meter equipment.
Cuvettes used with transmission spectrophotometers are designed with a front and a rear wall forming a right angle in relation to the optical axis of the light emitted from the light source. Cuvettes having such a design very often perform poorly with many products of interest for spectrophotometric analyses."The poor performance is normally related to the chemical and physi¬ cal nature and properties of the products, which cause large variations in the absorbency levels between different samples as well as between different measurements on the same sample. Varying structures of various samples imply similar problems due to varying density when charged in a cuvette. The samples can thus not be satisfactorily analysed using an equipment as herein disclosed.
It has by means of the present invention quite surprisingly been possible to eliminate or substantially reduce above prob¬ lems. The present invention refers to a spectrophotometric equipment comprising a light emitting source arranged in front of a cuvette and a detector arranged behind said cuvette, which detector measures the absorption of emitted light in a sample material introduced into the cuvette. The emitted light or a portion of the said light passes, prior to recording in the detector, through a transparent front wall of the cuvette, the sample material and a transparent rear wall of the cuvette. The equipment is characterised in that the front wall and/or the rear wall of the cuvette forms an oblique angle in relation to the optical axis of the emitted light. The cuvette can thereby be wedge-shaped, cylindrical, spherical or the like giving above oblique angle in relation to said optical axis.
At least the front and rear wall of the cuvette are made of glass, quartz or other material transparent to the wavelengths of used light.
The front wall of the cuvette is, according to one preferred embodiment of the invention, designed to give an oblique angle in relation to the optical axis of the light emitted from the light source, while the rear wall forms a right angle in rela¬ tion of said optical axis. The aforementioned embodiments of the cuvette according to the present invention give the major advantages of less variations in absorbency levels between different sample materials and types as well as a reduced difference between the highest and the lowest value of each spectra. A single transmission spect¬ rophotometer can thus with said cuvette at a very high degree of reliability analyse a large number of different materials.
Used light source normally works within the wavelength area of 800-1100 nm, which is near the infrared light.
The light that passes through the cuvette has a comparatively narrow divergence when entering the sample but rapidly diverges through reflection and change of direction as soon as it has entered into the sample material. Normally, 1/100 to 1/100000 of entered light passes through a finely powdered sample, which sample state is a preferred embodiment of the invention. It is often, in order to reproduce analytical results, necessary to prepare a sample material used in different analysis similarly or equally.
The design of the cuvette according to the present invention, furthermore, facilitates filling and packing of powdered mate¬ rials thereinto. The sample material can, of course, also be present as granules, a slurry, a paste, a liquid or the like.
The present invention also refers to the use of aforementioned spectrophotometric equipment for analyses of materials such as fodders and foodstuffs, whereby these products are available as granules, a powder, a slurry, a paste or a liquid or are trans¬ ferred into any of above states prior to the analysis. The use is, of course, not limited to above materials and/or states. The equipment according to the present invention can advanta¬ geously be used for analysis of all types of sample materials giving rise to discussed problems. The equipment according to the present invention is especially suitable for analyses of materials containing fat and/or protein.
The invention and the attendant advantages will be more fully understood from the following detailed description, taken in conjunction with appended Figure 1 showing an embodiment of a spectrophotometric equipment according to the present invention. The spectrophotometric equipment comprises a monochromatic light source 1 arranged in front of a cuvette 2 and a detector unit 2 arranged behind said cuvette 2. A finely powdered material 4, a fodder, is charged in the cuvette 2. Light emitted from the light source 1 will pass through a transparent front wall 5 of the cuvette 2, the fodder 4 and a transparent rear wall 6 of the cuvette 2 prior to measurement in the detector 3. The front wall 5 forms an oblique angle in relation to the optical axis 7 of the light.
Recorded light absorption will be used for calculation of dif¬ ferent parameters by means of statistical methods and models.
All obtained analytical results are, compared to analyses using a conventional cuvette, more accurate.
While a particular embodiment of the invention has been shown, it will be understood, of course, that the invention is not limited thereto since many modifications may be made, and it is, therefore, contemplated to cover by the appended claims any such modifications as fall within the true spirit and scope of the invention.

Claims

1. Spectrophotometric equipment comprising a light source (1) arranged in front of a cuvette (2) and a detector or detec¬ tor unit (3) arranged behind said cuvette (2), which detec¬ tor (3) measures absorption of light, emitted from the light source (1) , in a sample material (4) charged in the cuvette (2), whereby the light or a portion of the light passes through a front wall (5) of the cuvette (2), the sample material (4) and a rear wall (6) of the cuvette prior to measurement in the detector (3) c h a r a c t e r i s e d i n, that the front wall (5) and/or the rear wall (6) of the cuvette (2) forms an oblique angle in relation to an optical axis (7) of the light emitted from the light source (1).
Spectrophotometric equipment according to claim 1 c h a r a c t e r i s e d i n, that the front wall (5) of the cuvette (2) forms an oblique angle in relation to said optical axis (7) and that the rear wall (6) forms a substantially right angle in relation to said optical axis (7) .
3. Spectrophotometric equipment according to claim 1 or 2 c h a r a c t e r i s e d i n, that the cuvette is wedge-shaped having its largest distance between the front wall (5) and the rear wall (7) upwards
4. Spectrophotometric equipment according to any of the claims 1 - 3 c h a r a c t e r i s e d i n, that the light source (1) works within a wavelength area of 800 to 1100 nm.
5. Spectrophotometric equipment according to any of the claims 1 - 4 c h a r a c t e r i s e d i n, that the sample material (4) is present in form of granules, a powder, a slurry, a paste or a liquid.
6. Spectrophotometric equipment according to claim 5 c h a r a c t e r i s e d i n, that the sample material (4) is present in form of a finely grin¬ ded powder.
7. Spectrophotometric equipment according to any of the claims 1 - 6 c h a r a c t e r i s e d i n, that at least the front wall (5) and the rear wall (6) is made of a material being transparent to used wavelengths.
8. Spectrophotometric equipment according to claim 7 c h a r a c t e r i s e d i n, that at least the front wall (5) and the rear wall (6) is made of glass or quartz.
9. Use of a spectrophotometric equipment according to any of the claims 1 - 8 for analyses of a material being available in form of granules, a powder, a slurry, a paste or a liquid or for analyses of a material being transformed into granu¬ les, a powder, a slurry, a paste or a liquid prior to said analyses.
10. Use according to claim 9 c h a r a c t e r i s e d i n, that the material is a fodder or a foodstuff.
11. Use according to claim 9 or 10 c h a r a c t e r i s e d i n, that the material contains fat and/or protein.
PCT/SE1995/000808 1994-07-04 1995-06-30 Spectrophotometric equipment and use thereof WO1996001417A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU29413/95A AU2941395A (en) 1994-07-04 1995-06-30 Spectrophotometric equipment and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9402354-6 1994-07-04
SE9402354A SE502995C2 (en) 1994-07-04 1994-07-04 Spectrophotometer equipment and its use

Publications (1)

Publication Number Publication Date
WO1996001417A1 true WO1996001417A1 (en) 1996-01-18

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/SE1995/000808 WO1996001417A1 (en) 1994-07-04 1995-06-30 Spectrophotometric equipment and use thereof

Country Status (3)

Country Link
AU (1) AU2941395A (en)
SE (1) SE502995C2 (en)
WO (1) WO1996001417A1 (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5893288A (en) * 1997-04-25 1999-04-13 Morgan Construction Company Multiple outlet finishing mill
WO2000012997A1 (en) * 1998-08-26 2000-03-09 Alfa Laval Agri Ab Fluid sensing device and method for use in particular in milking machines
US6573988B1 (en) 1997-10-31 2003-06-03 Foss Electric A/S Cuvette and spacer therefor as well as a method of producing the spacer
CN110044829A (en) * 2019-05-08 2019-07-23 上海仪电物理光学仪器有限公司 Food quality analyzer and its application method
EP4049756A1 (en) * 2021-02-24 2022-08-31 Paul Marienfeld GmbH & Co. KG Container for a sample and method for analyzing a sample with the container

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4056324A (en) * 1975-05-10 1977-11-01 Hildegard Gohde Apparatus for counting and/or measuring particles suspended in a fluid medium
WO1982000358A1 (en) * 1980-07-24 1982-02-04 Oy Labsystems Method of measurement and a cuvette
EP0479231A1 (en) * 1990-10-01 1992-04-08 Canon Kabushiki Kaisha Apparatus and method for measuring specimen
WO1993015390A1 (en) * 1992-01-22 1993-08-05 Matthew Jonathan Rooney Assessment of characteristics of fluids

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4056324A (en) * 1975-05-10 1977-11-01 Hildegard Gohde Apparatus for counting and/or measuring particles suspended in a fluid medium
WO1982000358A1 (en) * 1980-07-24 1982-02-04 Oy Labsystems Method of measurement and a cuvette
EP0479231A1 (en) * 1990-10-01 1992-04-08 Canon Kabushiki Kaisha Apparatus and method for measuring specimen
WO1993015390A1 (en) * 1992-01-22 1993-08-05 Matthew Jonathan Rooney Assessment of characteristics of fluids

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5893288A (en) * 1997-04-25 1999-04-13 Morgan Construction Company Multiple outlet finishing mill
US6573988B1 (en) 1997-10-31 2003-06-03 Foss Electric A/S Cuvette and spacer therefor as well as a method of producing the spacer
WO2000012997A1 (en) * 1998-08-26 2000-03-09 Alfa Laval Agri Ab Fluid sensing device and method for use in particular in milking machines
CN110044829A (en) * 2019-05-08 2019-07-23 上海仪电物理光学仪器有限公司 Food quality analyzer and its application method
EP4049756A1 (en) * 2021-02-24 2022-08-31 Paul Marienfeld GmbH & Co. KG Container for a sample and method for analyzing a sample with the container
WO2022180089A3 (en) * 2021-02-24 2022-10-20 Paul Marienfeld GmbH & Co. KG Container for a sample and method for examining a sample with the container

Also Published As

Publication number Publication date
SE9402354L (en) 1996-01-05
SE502995C2 (en) 1996-03-04
SE9402354D0 (en) 1994-07-04
AU2941395A (en) 1996-01-25

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