WO1994000016A1 - Biochemical deactivation of rna viruses - Google Patents

Biochemical deactivation of rna viruses Download PDF

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Publication number
WO1994000016A1
WO1994000016A1 PCT/CH1992/000249 CH9200249W WO9400016A1 WO 1994000016 A1 WO1994000016 A1 WO 1994000016A1 CH 9200249 W CH9200249 W CH 9200249W WO 9400016 A1 WO9400016 A1 WO 9400016A1
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Prior art keywords
rna viruses
rnases
agents
rna
detergents
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PCT/CH1992/000249
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German (de)
French (fr)
Inventor
Urs V. Wirth
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Wirth Urs V
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Priority to AU32526/93A priority Critical patent/AU3252693A/en
Publication of WO1994000016A1 publication Critical patent/WO1994000016A1/en

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    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C11D3/38636—Preparations containing enzymes, e.g. protease or amylase containing enzymes other than protease, amylase, lipase, cellulase, oxidase or reductase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A61K8/66—Enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/48—Medical, disinfecting agents, disinfecting, antibacterial, germicidal or antimicrobial compositions

Definitions

  • RNases Ribonucleases
  • alRVh anti-infectives against RNA viruses
  • Agents with RNases can be combined with a wide variety of conventional substances (theoretically in all conceivable physical states and mixture types) for cleaning, disinfection or personal care or used as new optimized reagents in various combinations with new formulations.
  • Agents with RNases are suitable for disinfecting objects, such as equipment and clothing, from contaminating RNA viruses.
  • Agents with RNases applied to objects and especially to the skin surfaces, including mucous membranes, are suitable for protecting humans and / or animals from pathogenic RNA viruses.
  • Agents with RNases can also be used to control RNA viruses in crop protection.
  • RNA viruses The use of agents with RNases, combined with other substance mixtures as needed, therefore serves to prevent (prevent) diseases caused by RNA viruses.
  • This role can be described as anti-infectives against RNA viruses (aiRVi). This is independent of disease-treating (therapeutic) measures that are only used after infestation by pathogens.
  • Poliomyelitis viruses e.g. poliomyelitis
  • Coxsackieviruses e.g. meningitis
  • Rhinoviruses e.g. Colds and Colds - Encephalomyocarditis Virus:
  • - Arboviruses e.g. Encephalitis or encephalomyelitis
  • Ortho-M . e.g. Influe ⁇ zaviruses - Para-M .: e.g. Parainflue ⁇ za, Mumps, ...
  • Myxo-like viruses e.g. measles, rubella, distemper, rinderpest, swine fever, ...
  • Retroviruses RNA tumor viruses, ...
  • RSV e.g. Chicken sarcoma, poultry leukosis, MMLV: mouse leukemia, MMTV: mouse
  • Lentiviruses e.g. Visna (animals); HTLV (people); ...
  • RNA viruses also trigger undesirable damage in plants: (Source: F. Schönbeck, plant diseases, Teubner, 1979)
  • RNA viruses with RNases and detergents, possibly with additives for stabilizing and / or enhancing their activity / stability and possibly in combination with conventional substance mixtures.
  • RNA viruses anti-infectious gene RNA viruses
  • Agents with RNases can be combined with a wide variety of conventional substances for cleaning, disinfection or personal care or used as new optimized reagents in various combinations with new formulations:
  • At least one RNase e.g. RNaseA
  • a detergent e.g. Sodium Lauryl Sulfate
  • agents with RNases in all personal care products are suitable, such as: washing lotions, creams, hair shampoos, shower gels, powders, mouthwash, eye drops, intimate care products, ... etc.
  • these agents have a short-term inactivating effect on RNA viruses (disinfectant) or impart a longer-lasting inactivation ability against RNA viruses (long-lasting protective layer).
  • RNA viruses infectious agents
  • long-lasting protective layer the use of agents that leave a relatively resistant film (“protective film”) on the treated surfaces (devices, clothes, etc.) is recommended.
  • Such agents, based on gels, oils or waxes have already been patented in some cases and are used in practice.
  • aiRVi anti-infectives against JRNA viruses based on RNase (s) and suitable detergents
  • RNA-degrading enzymes ribonucleases (RNA-degrading enzymes)
  • RNase A EC 3.1.27.5 .: isolated from bovine pancreas; with the properties described above, available from Sigma (No L5125)
  • RNase T1 EC 3.1.27.3: isolated from Aspergillus oryzam
  • SLS Sodium lauryl sulphate

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Organic Chemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Environmental Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Dentistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Birds (AREA)
  • Dermatology (AREA)
  • Epidemiology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Detergent Compositions (AREA)
  • Cosmetics (AREA)

Abstract

Ribonucleases (RNases) are highly active, easily used and extremely stable enzymes. Together with detergents, RNases deactivate and destroy irreversible RNA viruses. Agents containing RNase(s) and detergents are therefore suitable for disinfecting instruments, clothing and surfaces from contaminating RNA viruses. Special application forms for coating the skin surfaces of human beings, animals or plants reduce the risk of infection from RNA viruses. Agents with RNases and detergents, possibly combined with substances, may be regarded as 'anti-infectives against RNA viruses (=aiRVi).

Description

Biochemische Inaktivierung von RNA-Viren.Biochemical inactivation of RNA viruses.
Ribonukleasen (RNasen) sind hochaktive, anspruchsarme und extrem stabile Enzyme. RNasen inaktivieren in Zusammenwirkung mit Tensiden (= Detergentien ) RNA-Viren rasch und irreversibel. Mischungen von RNasen & Detergentien (Mittel mit RNasen) als Basis zusammen mit im folgenden beschriebenen Substanzgemischen kann man als anti-infectiva gegen RNA-Viren (alRVh bezeichnen:Ribonucleases (RNases) are highly active, low-demanding and extremely stable enzymes. RNases quickly and irreversibly inactivate RNA viruses in conjunction with surfactants (= detergents). Mixtures of RNases & detergents (agents with RNases) as a base together with the substance mixtures described below can be described as anti-infectives against RNA viruses (alRVh):
Mittel mit RNasen können mit verschiedensten herkömmlichen Substanzen (theoretisch in allen denkbaren Aggregatzuständen und Mischungstypen) zur Reinigung, Desinfektion oder Körperpflege kombiniert oder als neue optimierte Reagentien in verschiedenen Kombinationen mit neuen Formulierungen angewendet werden.Agents with RNases can be combined with a wide variety of conventional substances (theoretically in all conceivable physical states and mixture types) for cleaning, disinfection or personal care or used as new optimized reagents in various combinations with new formulations.
Mittel mit RNasen sind geeignet Gegenstände, wie zum Beispiel Geräte und Kleidung von kontaminierenden RNA-Viren zu desinfizieren.Agents with RNases are suitable for disinfecting objects, such as equipment and clothing, from contaminating RNA viruses.
Mittel mit RNasen appliziert auf Gegenstände und vorallem auf die Hautoberflächen inklusive Schleimhäute eignen sich als Schutz für Mensch und/oder Tier vor krankheitserregenden RNA-Viren.Agents with RNases applied to objects and especially to the skin surfaces, including mucous membranes, are suitable for protecting humans and / or animals from pathogenic RNA viruses.
Mittel mit RNasen können auch zur Bekämpfung von RNA-Viren im Pflanzenschutz angewendet werden.Agents with RNases can also be used to control RNA viruses in crop protection.
Verwendung von Mittel mit RNasen, in Bedarf kombiniert mit anderen Substanzgemischen dienen deshalb zur Vorbeugung (Prävention) von Krankheiten verursacht durch RNA-Viren. Diese Rolle kann als anti-infectiva gegen RNA-Viren bezeichnet werden (aiRVi). Dies ist unabhängig von krankheitsbehandelden (therapeutischen) Massnahmen, die erst nach Befall durch Krankeitserreger eingesetzt werden.The use of agents with RNases, combined with other substance mixtures as needed, therefore serves to prevent (prevent) diseases caused by RNA viruses. This role can be described as anti-infectives against RNA viruses (aiRVi). This is independent of disease-treating (therapeutic) measures that are only used after infestation by pathogens.
a) Beispiele und Bedeutung einiger RNA-Viren:a) Examples and meaning of some RNA viruses:
RNA -Viren enthalten als Erbmaterial entweder meist auschliesslich oder seltener mindestens zu einem grossen Teil ribonucleic-acid ( RNA = RNS = Ribonucleinsäure .RNA viruses either contain exclusively or rarely at least to a large extent ribonucleic acid (RNA = RNS = ribonucleic acid) as genetic material.
a1) Unter den RNA-Viren findet man eine immense Anzahl von Erregern gravierender und oft tödlicher KrenKheiten frei Mensch und Tier; (Quellen: - : E. Wiesmann, Med. Mikrobiologie, Thieme-Veriag,1974; +: Referenzen auf Anfrage )a1) There is an immense number of pathogens causing serious and often fatal diseases free from humans and animals among the RNA viruses; (Sources: -: E. Wiesmann, Med. Microbiology, Thieme-Veriag, 1974; +: references on request)
- Picornaviren: -- Enteroviren:- Picornaviruses: - Enteroviruses:
- Poliomyelitis Viren: zB. Kinderlähmung- Poliomyelitis viruses: e.g. poliomyelitis
- Coxsackieviren: zB. Meningitis- Coxsackieviruses: e.g. meningitis
- ECHO Viren: zB. Meningitis & Meningoenzephalitis- ECHO viruses: e.g. Meningitis & Meningoencephalitis
- Rhinoviren: zB. Schnupfen und Erkältungen -- Encephalomyokarditisvirus:- Rhinoviruses: e.g. Colds and Colds - Encephalomyocarditis Virus:
-- Maul- und Klauenseucheviren:- Foot and mouth disease viruses:
- Reoviren: ?- Reoviruses:?
- Arboviren: zB. Enzephalitis oder Enzephalomyelitis- Arboviruses: e.g. Encephalitis or encephalomyelitis
- Myxoviren:- Myxoviruses:
- Ortho-M.: zB. Influeπzaviren - Para-M.: Bsp. Parainflueπza, Mumps,...- Ortho-M .: e.g. Influeπzaviruses - Para-M .: e.g. Parainflueπza, Mumps, ...
- Myxoähnliche Viren: Bsp. Masern, Röteln, Staupe, Rinderpest, Schweinepest,...- Myxo-like viruses: e.g. measles, rubella, distemper, rinderpest, swine fever, ...
+ gewisse Hepatitisviren: zB. Leberentzündung und -tumoren+ certain hepatitis viruses: e.g. Inflammation of the liver and tumors
+ Retroviren: v.a. RNA-Tumorviren,...Retroviruses: RNA tumor viruses, ...
++ RSV: zB. Hühnersarkom, Geflügelleukose, MMLV: Mäuseleukämie, MMTV: Maus-++ RSV: e.g. Chicken sarcoma, poultry leukosis, MMLV: mouse leukemia, MMTV: mouse
Mammakarzinom, ...Breast cancer, ...
++ Lentiviren: Bsp: Visna (Tiere); HTLV (Menschen);...++ Lentiviruses: e.g. Visna (animals); HTLV (people); ...
+++ HTLVIII = HIV = LAV: (Haupt-)Verursacher von AIDS (aquired immune deficiency) bei Mensch und ähnliche Viren bei Tieren: Bsp: SIV (simian), BIV (bovine), FIV (feline),...+++ HTLVIII = HIV = LAV: (main) cause of AIDS (aquired immune deficiency) in humans and similar viruses in animals: e.g. SIV (simian), BIV (bovine), FIV (feline), ...
a2) RNA-Viren lösen auch bei Pflanzen unerwünschte Schädigungen aus: (Quelle: F. Schönbeck, Pflanzenkrankheiten, Teubner, 1979)a2) RNA viruses also trigger undesirable damage in plants: (Source: F. Schönbeck, plant diseases, Teubner, 1979)
- Blatt-Mosaik-viren: Como-, Tobamo-, Bromo-, und weitere Gruppen.- Leaf mosaic viruses: Como, Tobamo, Bromo, and other groups.
- Rhabdoviren:- Rhabdoviruses:
- Reoviren:- Reoviruses:
b) Eigenschaften von RNasen am Beispiel RNase A:b) Properties of RNases using the example of RNase A:
- Stabilität: hitzestabil (volle Aktivität auch nach kochen!) und trockenstabil- Stability: heat stable (full activity even after cooking!) And dry stable
- Aktivität: wenig beeinflusst vom pH (5-8 optimal), von Salzkonzentrationen (0.3 Mol/I NaCI Optimum) und von Detergentien (z.B. SLS= Sodium Lauryl Sulphate). c1) Effekt von RNase und Detergentien aul RNA-Viren:- Activity: little influenced by pH (5-8 optimal), salt concentrations (0.3 mol / I NaCI Optimum) and detergents (e.g. SLS = Sodium Lauryl Sulphate). c 1 ) Effect of RNase and Detergents on RNA Viruses:
- RNA-Viren sind autgebaut aus ihrer Erbsubstanz RNA und einem Gerüstmantel (=Kapsid) au Proteinen. Gewisse RNA-Viren besitzen auch eine Membranhülle bestehend aus Lipiden und Proteinen.- RNA viruses are built from their genetic material RNA and a scaffold (= capsid) made of proteins. Certain RNA viruses also have a membrane envelope consisting of lipids and proteins.
- Mittel mit RNasen enthalten RNasen und Detergentien, evt. mit Zusätzen zur Stabilisierung und/oder Verstärkung derer Aktivität/Stabilität und evt. in Kombination mit herkömmlichen Substanzgemischen. Als anti-infectiva genen RNA-Viren (aiRN/i) bewirken solche Mittel folgen RNA-Viren inaktivierende Effekte: Detergentien (= Tenside = Amphiphiie Verbindungen) löse die Membranhülle auf und brechen den Gerüstmantel auf, danach zersetzen (hydrolisieren) RNasen die Erbsubstanz der RNA-Viren, was zu einer vollständigen Vernichtung der RNA-Vir führt. Damit werden nicht nur Aufnahmefähigkeit durch den Organismus, sondern auch jeglich Vermehrungspotential der RNA-Viren vollständig eliminiert. Detergentien allein können die Inf ektiosität von RNA-Viren bis zu einem gewissen Masse auch reduzieren. Die neuartige Vernichtung der Erbsubstanz RNA durch Mittel mit RNasen zur vollständigen Inaktivierung ist besonderer Bedeutung, da kürzlich experimentell gezeigt wurde, dass zum Beispiel Polio Vir sich allein mit ihrer RNA in intakten oder sogar in aufgelösten Zellen noch vermehren (Science News, Vol.140, p.390, 1991).- Agents with RNases contain RNases and detergents, possibly with additives for stabilizing and / or enhancing their activity / stability and possibly in combination with conventional substance mixtures. As anti-infectious gene RNA viruses (aiRN / i) such agents act to have RNA-virus inactivating effects: detergents (= surfactants = amphiphiic compounds) dissolve the membrane shell and break open the scaffold, then RNases decompose (hydrolize) the genetic material of the RNA viruses, which leads to a complete destruction of the RNA vir. This completely eliminates not only the ability of the organism to absorb, but also any potential for multiplication of the RNA viruses. Detergents alone can also reduce the infectivity of RNA viruses to a certain extent. The novel destruction of the genetic material RNA by means of RNases for complete inactivation is of particular importance, since it has recently been shown experimentally that, for example, Polio Vir alone can multiply with its RNA in intact or even in dissolved cells (Science News, Vol.140, p.390, 1991).
ό' Mittel mit RNasen:Mittel ' means with RNases:
Mittel mit RNasen können mit verschiedensten herkömmlichen Substanzen zur Reinigung, Desinfektion oder Körperpflege kombiniert oder als neue optimierte Reagentien in verschiedenen Kombinationen mit neuen Formulierungen angewendet werden:Agents with RNases can be combined with a wide variety of conventional substances for cleaning, disinfection or personal care or used as new optimized reagents in various combinations with new formulations:
- Zur Reinigung und Desinfektion braucht es mindestens eine RNase (zB. RNaseA) und ein Detergens (z.B. Sodium Lauryl Sulfate). Von der Grundlagenforschung ist bekannt, dass RNasen unter verschiedensten Bedingungen wirkungsvoll arbeiten.- At least one RNase (e.g. RNaseA) and a detergent (e.g. Sodium Lauryl Sulfate) are required for cleaning and disinfection. It is known from basic research that RNases work effectively under various conditions.
- Für den Schutz vor Infektion eignen sich Mittel mit RNasen in allen Körperpflegemitteln, wie zum Beispiel: Waschlotions, Cremes, Haar-Shampoos, Dusch-Gels, Puders, Mundwasser, Augentropfen, Intim-Pflegemittel, ...etc. Je nach Zusammensetzung wirken diese Mittel kurzfristig inaktivierend auf RNA-Viren (desinfizierend) oder vermitteln einen länger anhaltend Inaktivierungsfähigkeit gegen RNA-Viren (ausdauerende Schutzschicht). Für eine erwünscht längere protektive Wirkung empfiehlt sich die Anwendung von Mitteln, die eine relativ widerstandsfähigen Film ("Schutzfilm") auf den behandelten Oberflächen (Geräte, Kleider, Ha hinterlassen. Solche Mittel, basierend auf Gelen, Oelen oder Wachsen sind schon zum Teil patentiert und werden in der Praxis angewendet.
Figure imgf000006_0001
- To protect against infection, agents with RNases in all personal care products are suitable, such as: washing lotions, creams, hair shampoos, shower gels, powders, mouthwash, eye drops, intimate care products, ... etc. Depending on the composition, these agents have a short-term inactivating effect on RNA viruses (disinfectant) or impart a longer-lasting inactivation ability against RNA viruses (long-lasting protective layer). For a desired longer protective effect, the use of agents that leave a relatively resistant film ("protective film") on the treated surfaces (devices, clothes, etc.) is recommended. Such agents, based on gels, oils or waxes, have already been patented in some cases and are used in practice.
Figure imgf000006_0001
f. Anhang:f. Attachment:
erfindungsgem. Mittel: bewirkt: Anwendung:according to the invention. Medium: causes: application:
RNasen + Detergentien Inaktivierung von - Reinigung und Desinfektion (Mittel mit RNasen) RNA-Viren als von Geräten und Kleidung.RNases + detergents Inactivation of - cleaning and disinfection (agents with RNases) RNA viruses as of devices and clothing.
Krankheitserreger - Schutz und PräventionPathogens - protection and prevention
Mensch, Tier & bei Mensch, Tier & Pflanze.Humans, animals and humans, animals and plants.
Pflanzeplant
Beispiele: Enzephalitis,Examples: encephalitis,
Tumoren und AIDS.Tumors and AIDS.
aiRVi = anti-infectiva gegen JRNA-Vjren, basierend auf RNase(-n) und geeigneten/m Detergentien/iumaiRVi = anti-infectives against JRNA viruses based on RNase (s) and suitable detergents
- RNasen = Ribonukleasen (RNA abbauende Enzyme)- RNases = ribonucleases (RNA-degrading enzymes)
- Detergentium (=Tensid = amphiphile Substanz)- Detergent (= surfactant = amphiphilic substance)
geeignet sind zum Beispiel:suitable examples are:
- RNase A: EC 3.1.27.5.: isoliert von bovinen Pankreas; mit obig beschriebenen Eigenschaften, käuflich bei Sigma (No L5125)RNase A: EC 3.1.27.5 .: isolated from bovine pancreas; with the properties described above, available from Sigma (No L5125)
- RNase T1 : EC 3.1.27.3: isoliert aus Aspergillus oryzamRNase T1: EC 3.1.27.3: isolated from Aspergillus oryzam
- weitere RNasen aus Tier-, Pflanzen oder Mikrobenreich kommen in Frage- Further RNases from animal, plant or microbial realm are possible
- Detergentium: geignet ist zB. Sodium Lauryl Sulphate (SLS), der Effekt von Detergentien kann nötigenfalls durch Zusätze verstärkt werden, wobei diese aber möglichst wenig die Aktivität und evt. Stabilität der RNase(-n) beinträchtigen sollten. - Detergent: is suitable, for example. Sodium lauryl sulphate (SLS), the effect of detergents can be enhanced if necessary, but these should have as little impact as possible on the activity and possibly stability of the RNase (s).

Claims

PATENTANSPRUECHE: PATENT CLAIMS:
1. Mittel zur biochemischen Inaktivierung von RNA-Viren, als Bsis bestehend aus Ribonuklease(-n) =RNase(-n) zusammen mit mindestens einem Detergentium.1. Agent for the biochemical inactivation of RNA viruses, as a Bsis consisting of ribonuclease (-n) = RNase (-n) together with at least one detergent.
2. Mittel nach Anspruch 1 , dadurch gekennzeichnet, dass sie in Form von Reinigungsmittel, Desinfektionsmittel, Körperpflegemittel oder Beschichtungsmittel vorliegen.2. Composition according to claim 1, characterized in that they are in the form of cleaning agents, disinfectants, personal care products or coating agents.
3. Mittel nach Anspruch 1 , zur Verwendung als Schutzmittel für Mensch, Tier und/oder Pflanze vor Infektion durch RNA-Viren. 3. Composition according to claim 1, for use as a protective agent for humans, animals and / or plants from infection by RNA viruses.
GEÄNDERTE ANSPRÜCHECHANGED REQUIREMENTS
[beim Internationalen Büro am 4. Oktober 1993 (04.10.93) eingegangen; neuer Anspruch 4 hinzugefügt; alle weiteren Ansprüche unverändert[Received at the International Bureau on October 4, 1993 (October 4, 1993); added new claim 4; all other claims unchanged
(1 Seite)](1 page)]
1. Mittel zur biochemischen Inaktivierung von RNA-Viren, bestehend aus Ribonuklease(-n) =RNase(-n) zusammen mit mindestens einem Detergentium.1. Agent for the biochemical inactivation of RNA viruses, consisting of ribonuclease (-n) = RNase (-n) together with at least one detergent.
2. Mittel nach Anspruch 1 , dadurch gekennzeichnet, dass sie in Form von Reinigungsmittel, Desinfektionsmittel, Körperpflegemittel oder Beschichtungsmittel vorliegen.2. Composition according to claim 1, characterized in that they are in the form of cleaning agents, disinfectants, personal care products or coating agents.
3. Mittel nach Anspruch 1 , zur Verwendung als Schutzmittel für Mensch, Tier und/oder Pflanze vor Infektion durch RNA-Viren.3. Composition according to claim 1, for use as a protective agent for humans, animals and / or plants from infection by RNA viruses.
4. Mittel nach Anspruch 1 , zur zielgerichteten und lokalen Bekämpfung von Infektionskrankheiten durch RNA- und DNA-Virus Infektionen oder lokalisierten Tumoren bei Mensch und Tieren. 4. Means according to claim 1, for targeted and local control of infectious diseases by RNA and DNA virus infections or localized tumors in humans and animals.
PCT/CH1992/000249 1992-06-23 1992-12-31 Biochemical deactivation of rna viruses WO1994000016A1 (en)

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CH1957/92A CH682541A5 (en) 1992-06-23 1992-06-23 Biochemical Inactivation of RNA viruses.

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US9969885B2 (en) 2014-07-31 2018-05-15 Kimberly-Clark Worldwide, Inc. Anti-adherent composition
US10028899B2 (en) 2014-07-31 2018-07-24 Kimberly-Clark Worldwide, Inc. Anti-adherent alcohol-based composition
US10238107B2 (en) 2014-07-31 2019-03-26 Kimberly-Clark Worldwide, Inc. Anti-adherent composition
WO2020070014A1 (en) * 2018-10-02 2020-04-09 Novozymes A/S Cleaning composition comprising anionic surfactant and a polypeptide having rnase activity
US11168287B2 (en) 2016-05-26 2021-11-09 Kimberly-Clark Worldwide, Inc. Anti-adherent compositions and methods of inhibiting the adherence of microbes to a surface
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