US3859049A - Blood reference standard and process for blood gas test - Google Patents
Blood reference standard and process for blood gas test Download PDFInfo
- Publication number
- US3859049A US3859049A US438866A US43886674A US3859049A US 3859049 A US3859049 A US 3859049A US 438866 A US438866 A US 438866A US 43886674 A US43886674 A US 43886674A US 3859049 A US3859049 A US 3859049A
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- United States
- Prior art keywords
- reference standard
- blood
- fluoride
- citrate
- hemoglobin
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- Expired - Lifetime
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- 210000004369 blood Anatomy 0.000 title claims abstract description 40
- 239000008280 blood Substances 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims description 17
- JDNTWHVOXJZDSN-UHFFFAOYSA-N iodoacetic acid Chemical compound OC(=O)CI JDNTWHVOXJZDSN-UHFFFAOYSA-N 0.000 claims abstract description 20
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 claims abstract description 17
- KRHYYFGTRYWZRS-UHFFFAOYSA-M Fluoride anion Chemical compound [F-] KRHYYFGTRYWZRS-UHFFFAOYSA-M 0.000 claims abstract description 14
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical group [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 claims description 22
- QEWYKACRFQMRMB-UHFFFAOYSA-N fluoroacetic acid Chemical compound OC(=O)CF QEWYKACRFQMRMB-UHFFFAOYSA-N 0.000 claims description 17
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 15
- 102000001554 Hemoglobins Human genes 0.000 claims description 12
- 108010054147 Hemoglobins Proteins 0.000 claims description 12
- 239000011775 sodium fluoride Substances 0.000 claims description 11
- 235000013024 sodium fluoride Nutrition 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 210000000601 blood cell Anatomy 0.000 claims description 7
- 210000004027 cell Anatomy 0.000 claims description 6
- 239000001509 sodium citrate Substances 0.000 claims description 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 239000008103 glucose Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 2
- 239000007789 gas Substances 0.000 description 13
- 239000000203 mixture Substances 0.000 description 4
- 230000000087 stabilizing effect Effects 0.000 description 3
- 239000000337 buffer salt Substances 0.000 description 2
- 230000019522 cellular metabolic process Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 150000001450 anions Chemical class 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000009325 pulmonary function Effects 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/96—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/795—Porphyrin- or corrin-ring-containing peptides
- G01N2333/805—Haemoglobins; Myoglobins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2496/00—Reference solutions for assays of biological material
- G01N2496/05—Reference solutions for assays of biological material containing blood cells or plasma
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2496/00—Reference solutions for assays of biological material
- G01N2496/70—Blood gas control solutios containing dissolved oxygen, bicarbonate and the like
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/102499—Blood gas standard or control
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/105831—Protein or peptide standard or control [e.g., hemoglobin, etc.]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/108331—Preservative, buffer, anticoagulant or diluent
Definitions
- Blood gas tests are performed in most hospital laboratories on an emergency basis forthe purpose of diagnosing and treating abnormalities of pulmonary function and acid base balance.
- the three parameters are blood pH, pCO and p
- blood is drawn from the patient and introduced promptly into specialized equipment containing electrodes specific for each of the three blood-gas componetns. Since the blood sample deteriorates rapidly, it must be analyzed promptly, or within several hours if iced. As time progresses, however, the pH of the blood sample decreases, the pCO increases, and the p0 decreases as a result of cellular metabolism. There is no recognized process in the prior art for preventing these changes from occurring in stored blood samples.
- the present invention is directed to a means of stabilizing the aforesaid components in blood, or a suspension of blood cells in an appropriate media, so as to facilitate the use of stored blood for standardizing and controlling the blood gas equipment.
- the process of the invention may also be used to stabilize, for example, a mixture of hemoglobin and buffer salts, which does not involve blood cells.
- the stabilizing agents are fluoride, citrate and iodoacetate. Each of these substances prevents a portion of the cellular metabolic processes which cause the changes in blood gases noted previously: fluoride by binding magnesium; citrate and iodoacetate by inhibiting different pathways in the metabolic processes.
- Fresh blood is drawn and mixed with a solution containing concentrates of sodium fluoride, citrate and iodoacetic or fluoroacetic acid in sufficient quantity to yield the final blood concentrations mentioned above.
- a solution containing concentrates of sodium fluoride, citrate and iodoacetic or fluoroacetic acid in sufficient quantity to yield the final blood concentrations mentioned above.
- 25 milligrams of sodium fluoride and 93 milligrams of iodoacetic or fluoroacetic acid are dissolved in 10 ml of water and then mixed with 250 ml of blood.
- the citrate is also dissolved in water and mixed with the blood.
- These anions may be added in combination with any cation so long as the pH is ad justed to approximately 7.4 37C. Following this the blood is stored in air tight containers ideally leaving little or no air space.
- fresh red cells are washed and suspended in an albumen glucose solution containing concentrates of sodium fluoride and citrate in sufficient quantity to yield the concentrations set forth above.
- a mixture of hemoglobin (not cells) and buffer salts may be treated in the manner described above to stabilize the pH at about 7.40, the pCO at about 40 mm, and the p0 at about 80mm.
- Glycerol may be added to increase the viscosity of the mixture to about 3.5 centipoise, that of normal whole blood, and to act as a preservative.
- About a 50 percent solution at 37 C would have a viscosity of 3.5 centipoise, a percent solution would have better preserving power, but the viscosity would be 9.4 centipoise.
- a process of providing a reference standard which consists of deriving a reference standard containing blood, blood cells, hemoglobin, or the like, dissolving sodium fluoride and iodoacetic or fluroacetic acid in water, and mixing the resulting solution with the reference standard.
- a process for providing a reference standard which consists of washing fresh red cells, dissolving sodium fluoride and citrate in an albumen glucose solution, and suspending the red cells in the solution.
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
A stable blood reference standard and control for blood gas tests which includes the use of fluoride, citrate and iodoacetate.
Description
United States Patent Ware et a1.
Jan. 7, 1975 BLOOD REFERENCE STANDARD AND PROCESS FOR BLOOD GAS TEST Inventors: Arnold G. Ware, 4044 Park Vista pn, Pasadena, Calif. 91107; Edward P. Marbach, 4607 Marwood Dr., Los Angeles, Calif.
Filed: Feb. 1, 1974 Appl. No.: 438,866
Related US. Application Data Continuation-impart of Ser. No. 397,347, Sept. 14, 1973, abandoned.
US. Cl. 23/230 B, 252/408 Int. Cl. GOln 33/16 [58] Field of Search 23/230 B; 252/408; 424/11 Primary Examiner-R E. Serwin Attorney, Agent, or Firm-Jessup & Beecher [57] ABSTRACT A stable blood reference standard and control for blood gas tests which includes the use of fluoride, citrate and iodoacetate.
11 Claims, N0 Drawings BLOOD REFERENCE STANDARD AND PROCESS FOR BLOOD GAS TEST This is a continuation-in-part of Copending application Ser. No. 397,347, filed Sept. 14, 1973, and now abandoned.
BACKGROUND OF THE DISCLOSURE Blood gas tests are performed in most hospital laboratories on an emergency basis forthe purpose of diagnosing and treating abnormalities of pulmonary function and acid base balance. The three parameters are blood pH, pCO and p In performing the test, blood is drawn from the patient and introduced promptly into specialized equipment containing electrodes specific for each of the three blood-gas componetns. Since the blood sample deteriorates rapidly, it must be analyzed promptly, or within several hours if iced. As time progresses, however, the pH of the blood sample decreases, the pCO increases, and the p0 decreases as a result of cellular metabolism. There is no recognized process in the prior art for preventing these changes from occurring in stored blood samples.
Present day blood gas equipment is standardized and controlled by cumbersome procedures using buffers for pH, and gases for pCO and p0 Blood specimens of known pH, pCO and p0 would be preferable for this purpose. However, the instability of these components in blood has made this approach impractical in the prior art.
DETAILED DESCRIPTION OF THE INVENTION The present invention is directed to a means of stabilizing the aforesaid components in blood, or a suspension of blood cells in an appropriate media, so as to facilitate the use of stored blood for standardizing and controlling the blood gas equipment. The process of the invention may also be used to stabilize, for example, a mixture of hemoglobin and buffer salts, which does not involve blood cells. The stabilizing agents are fluoride, citrate and iodoacetate. Each of these substances prevents a portion of the cellular metabolic processes which cause the changes in blood gases noted previously: fluoride by binding magnesium; citrate and iodoacetate by inhibiting different pathways in the metabolic processes.
It has been found that combinations of the abovementioned inhibitors when added to blood or a suspension of blood cells, or a mixture of hemogloblin, stabilize the blood gases for periods of 30 days or longer when stored in closed containers under refrigeration.
The following example will further illustrate the present invention although the invention is not limited to this specific example which is provided by way of illustration and not limitation.
Fluoride 25 millimolar Citrate l2 millimolar iodoacetate 5 millimolar (fluoroacetate may be substituted for iodoacetate) It has been found that the concentrations listed above have been most efficient. However, it has also been found that the listed components may be varied considerably in concentration and in relation with each other without appreciably varying the blood gas stabilizing effect. For example, the following ranges of concentration have been found to be permissible, without significantly reducing the blood gas stabilizing effect.
Fluoride 10 to I00 millimolar Citrate 3 to 50 millimolar lodoacetate l to l0 millimolar In carrying out the process of the invention in accordance with one particular example, the following steps are presented:
Fresh blood is drawn and mixed with a solution containing concentrates of sodium fluoride, citrate and iodoacetic or fluoroacetic acid in sufficient quantity to yield the final blood concentrations mentioned above. For example, 25 milligrams of sodium fluoride and 93 milligrams of iodoacetic or fluoroacetic acid are dissolved in 10 ml of water and then mixed with 250 ml of blood. The citrate is also dissolved in water and mixed with the blood. These anions may be added in combination with any cation so long as the pH is ad justed to approximately 7.4 37C. Following this the blood is stored in air tight containers ideally leaving little or no air space. After 2 weeks at refrigerated temperature the pH, pCO and p0 are stabilized and remain unchanged for 30 days or longer if kept under refrigeration. In contrast, blood specimens without these preservatives show a progressive drop in pH and p0 and increase in pCO Such changes are sufficient in magnitude to render untreated blood unsatisfactory as a blood gas standard or control.
As another example, fresh red cells are washed and suspended in an albumen glucose solution containing concentrates of sodium fluoride and citrate in sufficient quantity to yield the concentrations set forth above.
As another example, a mixture of hemoglobin (not cells) and buffer salts may be treated in the manner described above to stabilize the pH at about 7.40, the pCO at about 40 mm, and the p0 at about 80mm. Glycerol may be added to increase the viscosity of the mixture to about 3.5 centipoise, that of normal whole blood, and to act as a preservative. About a 50 percent solution at 37 C would have a viscosity of 3.5 centipoise, a percent solution would have better preserving power, but the viscosity would be 9.4 centipoise.
It will be readily apparent to those skilled in the particular art under consideration that other examples of the invention described herein may be devised from an understanding of the foregoing specification without departing from the spirit and scope of the invention. It is intended in the following claims to cover all such modifications, variations and adaptations which are included within the scope of the invention.
What is claimed is:
l. A reference standard containing blood, blood cells, hemoglobin, or the like, reconstituted by the addition of a fluoride and an iodoacetate or a fluoroacetate.
2. A reference standard defined in claim 1, in which the fluoride and iodoacetate or fluoroacetate are included with a concentration for the fluoride substantially in a range of 10-100 millimolar, and with a concentration for the iodoacetate or fluoroacetate substantially in a range of l to 10 millimolar.
3. The reference standard defined in claim 1, and which also contains a citrate.
4. The reference standard defined in claim 2, and which further includes a citrate substantially in a range of 3 to 50 millimolar.
5. The reference standard defined in claim 1, in which the fluoride is sodium fluoride and the iodoacetate or fluoroacetate is iodoacetic or fluoroacetic acid.
6. The reference standard defined in claim 1, which comprises hemoglobin and glycerol in proportions to provide a viscosity of the order of 3.5 centipoise.
7. A process of providing a reference standard which consists of deriving a reference standard containing blood, blood cells, hemoglobin, or the like, dissolving sodium fluoride and iodoacetic or fluroacetic acid in water, and mixing the resulting solution with the reference standard.
8. The process defined in claim 7, and which further comprises the step of dissolving citrate in water and mixing the resulting solution with the reference standard.
9. The process defined in claim 7, in which 25 milligrams of sodium fluoride and 93 milligrams of iodoacetic acid are dissolved in 10 ml of water and then mixed with 25 ml of the reference standard or in multiples or sub-multiples thereof.
10. A process for providing a reference standard which consists of washing fresh red cells, dissolving sodium fluoride and citrate in an albumen glucose solution, and suspending the red cells in the solution.
11. The process defined in claim 7, which comprises deriving a unit of hemoglobin, and adding glycerol to the hemoglobin to increase the viscosity thereof to the order of 3.5 centipoise.
Claims (11)
1. A REFERENCE STANDARD CONTAINING BLOOD, BLOOD CELLS, HEMOGLOBIN, OR THE LIKE, RECONSTITUED BY THE ADDITION OF A FLUORIDE AND AN IODOACETATE OR FLUOROACETATE.
2. A reference standard defined in claim 1, in which the fluoride and iodoacetate or fluoroacetate are included with a concentration for the fluoride substantially in a range of 10-100 millimolar, and with a concentration for the iodoacetate or fluoroacetate substantially in a range of 1 to 10 millimolar.
3. The reference standard defined in claim 1, and which also contains a citrate.
4. The reference standard defined in claim 2, and which further includes a citrate substantially in a range of 3 to 50 millimolar.
5. The reference standard defined in claim 1, in which the fluoride is sodium fluoride and the iodoacetate or fluoroacetate is iodoacetic or fluoroacetic acid.
6. The reference standard defined in claim 1, which comprises hemoglobin and glycerol in proportions to provide a viscosity of the order of 3.5 centipoise.
7. A process of providing a reference standard which consists of deriving a reference standard containing blood, blood cells, hemoglobin, or the like, dissolving sodium fluoride and iodoacetic or fluroacetic acid in water, and mixing the resulting solution with the reference standard.
8. The process defined in claim 7, and whiCh further comprises the step of dissolving citrate in water and mixing the resulting solution with the reference standard.
9. The process defined in claim 7, in which 25 milligrams of sodium fluoride and 93 milligrams of iodoacetic acid are dissolved in 10 ml of water and then mixed with 25 ml of the reference standard or in multiples or sub-multiples thereof.
10. A process for providing a reference standard which consists of washing fresh red cells, dissolving sodium fluoride and citrate in an albumen glucose solution, and suspending the red cells in the solution.
11. The process defined in claim 7, which comprises deriving a unit of hemoglobin, and adding glycerol to the hemoglobin to increase the viscosity thereof to the order of 3.5 centipoise.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US438866A US3859049A (en) | 1973-09-14 | 1974-02-01 | Blood reference standard and process for blood gas test |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US39734773A | 1973-09-14 | 1973-09-14 | |
US438866A US3859049A (en) | 1973-09-14 | 1974-02-01 | Blood reference standard and process for blood gas test |
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US3859049A true US3859049A (en) | 1975-01-07 |
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US438866A Expired - Lifetime US3859049A (en) | 1973-09-14 | 1974-02-01 | Blood reference standard and process for blood gas test |
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Cited By (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3977995A (en) * | 1974-10-17 | 1976-08-31 | Baxter Laboratories, Inc. | Calibrating fluid for blood cell counting and hemoglobin determination |
US4001142A (en) * | 1975-07-25 | 1977-01-04 | Warner-Lambert Company | Blood gas control |
US4040785A (en) * | 1976-10-18 | 1977-08-09 | Technicon Instruments Corporation | Lysable blood preservative composition |
US4054488A (en) * | 1975-08-14 | 1977-10-18 | Marbach Edward P | Preservation of glucose in blood samples |
US4102810A (en) * | 1975-01-13 | 1978-07-25 | Coulter Electronics, Inc. | Stabilized hematological reagent solutions |
US4116336A (en) * | 1975-05-30 | 1978-09-26 | Radiometer A/S | Package containing a reference liquid for blood gas equipment |
US4126575A (en) * | 1977-11-22 | 1978-11-21 | Louderback Allan Lee | Blood control standard |
US4151108A (en) * | 1976-01-27 | 1979-04-24 | Radiometer A/S | Reference liquid for blood gas equipment |
US4163734A (en) * | 1975-05-30 | 1979-08-07 | Radiometer A/S | Reference liquid for blood gas equipment |
US4279775A (en) * | 1979-12-31 | 1981-07-21 | Louderback Allan Lee | Blood gas control |
US4351743A (en) * | 1976-04-07 | 1982-09-28 | Yoshikazu Hashimoto | Dilute standard gases prepared by utilizing buffered solution, method for preparation thereof and apparatus therefor |
WO1983003902A1 (en) * | 1982-05-03 | 1983-11-10 | American Hospital Supply Corporation | Blood gas control |
US4469792A (en) * | 1980-12-31 | 1984-09-04 | Allied Corporation | Blood gas calibration and control fluid utilizing stroma-free hemoglobin |
US4485174A (en) * | 1982-09-29 | 1984-11-27 | Instrumentation Laboratory Inc. | Hemoglobin-based blood gas control |
EP0181033A1 (en) * | 1984-11-05 | 1986-05-14 | Akzo N.V. | Control for blood gas analyzers and hemoglobin analysis |
US5308767A (en) * | 1986-10-31 | 1994-05-03 | Fuji Photo Film Co., Ltd. | Method for control or calibration in a chemical analytical determination |
US5422278A (en) * | 1987-11-17 | 1995-06-06 | Dade International Inc. | Blood gas/electrolyte calibrator and quality controls |
US5547874A (en) * | 1986-10-31 | 1996-08-20 | Fuji Photo Film Co., Ltd. | Method for control or calibration in a chemical analytical determination |
US5697366A (en) * | 1995-01-27 | 1997-12-16 | Optical Sensors Incorporated | In situ calibration system for sensors located in a physiologic line |
EP2116611A1 (en) * | 2007-01-30 | 2009-11-11 | Arkray, Inc. | Measurement method for hba1c |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3466249A (en) * | 1967-02-13 | 1969-09-09 | Baxter Laboratories Inc | Blood serum reference standard for multi-automated analytical procedures |
US3629142A (en) * | 1969-12-08 | 1971-12-21 | Edward P Marbach | Reference standard blood serum for the calibration of automatic blood serum analyzing apparatus |
US3681255A (en) * | 1970-09-03 | 1972-08-01 | Gen Electric | Process for the preparation of liquid calibration fluids |
-
1974
- 1974-02-01 US US438866A patent/US3859049A/en not_active Expired - Lifetime
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3466249A (en) * | 1967-02-13 | 1969-09-09 | Baxter Laboratories Inc | Blood serum reference standard for multi-automated analytical procedures |
US3629142A (en) * | 1969-12-08 | 1971-12-21 | Edward P Marbach | Reference standard blood serum for the calibration of automatic blood serum analyzing apparatus |
US3681255A (en) * | 1970-09-03 | 1972-08-01 | Gen Electric | Process for the preparation of liquid calibration fluids |
Cited By (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3977995A (en) * | 1974-10-17 | 1976-08-31 | Baxter Laboratories, Inc. | Calibrating fluid for blood cell counting and hemoglobin determination |
US4102810A (en) * | 1975-01-13 | 1978-07-25 | Coulter Electronics, Inc. | Stabilized hematological reagent solutions |
US4116336A (en) * | 1975-05-30 | 1978-09-26 | Radiometer A/S | Package containing a reference liquid for blood gas equipment |
US4163734A (en) * | 1975-05-30 | 1979-08-07 | Radiometer A/S | Reference liquid for blood gas equipment |
US4001142A (en) * | 1975-07-25 | 1977-01-04 | Warner-Lambert Company | Blood gas control |
DK152520B (en) * | 1975-07-25 | 1988-03-07 | Warner Lambert Co | REFERENCE SYSTEM FOR BLOOD GAS MEASUREMENTS AND PROCEDURES FOR PRODUCING THE SYSTEM |
US4054488A (en) * | 1975-08-14 | 1977-10-18 | Marbach Edward P | Preservation of glucose in blood samples |
US4151108A (en) * | 1976-01-27 | 1979-04-24 | Radiometer A/S | Reference liquid for blood gas equipment |
US4351743A (en) * | 1976-04-07 | 1982-09-28 | Yoshikazu Hashimoto | Dilute standard gases prepared by utilizing buffered solution, method for preparation thereof and apparatus therefor |
US4040785A (en) * | 1976-10-18 | 1977-08-09 | Technicon Instruments Corporation | Lysable blood preservative composition |
US4126575A (en) * | 1977-11-22 | 1978-11-21 | Louderback Allan Lee | Blood control standard |
US4279775A (en) * | 1979-12-31 | 1981-07-21 | Louderback Allan Lee | Blood gas control |
US4469792A (en) * | 1980-12-31 | 1984-09-04 | Allied Corporation | Blood gas calibration and control fluid utilizing stroma-free hemoglobin |
WO1983003902A1 (en) * | 1982-05-03 | 1983-11-10 | American Hospital Supply Corporation | Blood gas control |
US4458021A (en) * | 1982-05-03 | 1984-07-03 | American Hospital Supply Corporation | Blood gas control |
US4485174A (en) * | 1982-09-29 | 1984-11-27 | Instrumentation Laboratory Inc. | Hemoglobin-based blood gas control |
US4711852A (en) * | 1984-11-05 | 1987-12-08 | Akzo N.V. | Control for blood gas analyzers and hemoglobin analysis |
EP0181033A1 (en) * | 1984-11-05 | 1986-05-14 | Akzo N.V. | Control for blood gas analyzers and hemoglobin analysis |
US5308767A (en) * | 1986-10-31 | 1994-05-03 | Fuji Photo Film Co., Ltd. | Method for control or calibration in a chemical analytical determination |
US5547874A (en) * | 1986-10-31 | 1996-08-20 | Fuji Photo Film Co., Ltd. | Method for control or calibration in a chemical analytical determination |
US5422278A (en) * | 1987-11-17 | 1995-06-06 | Dade International Inc. | Blood gas/electrolyte calibrator and quality controls |
US5518929A (en) * | 1987-11-17 | 1996-05-21 | Dade International Inc. | Method of making a blood gas/electrolyte control |
US5697366A (en) * | 1995-01-27 | 1997-12-16 | Optical Sensors Incorporated | In situ calibration system for sensors located in a physiologic line |
US5976085A (en) * | 1995-01-27 | 1999-11-02 | Optical Sensors Incorporated | In situ calibration system for sensors located in a physiologic line |
EP2116611A1 (en) * | 2007-01-30 | 2009-11-11 | Arkray, Inc. | Measurement method for hba1c |
US20100178659A1 (en) * | 2007-01-30 | 2010-07-15 | Arkray, Inc. | METHOD OF MEASURING HbA1c |
US8008085B2 (en) * | 2007-01-30 | 2011-08-30 | Arkray, Inc. | Method of measuring HbA1c |
EP2116611A4 (en) * | 2007-01-30 | 2012-01-18 | Arkray Inc | Measurement method for hba1c |
JP5324918B2 (en) * | 2007-01-30 | 2013-10-23 | アークレイ株式会社 | HbA1c measurement method |
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