US20180193830A1 - Biopsy sample container - Google Patents
Biopsy sample container Download PDFInfo
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- US20180193830A1 US20180193830A1 US15/913,287 US201815913287A US2018193830A1 US 20180193830 A1 US20180193830 A1 US 20180193830A1 US 201815913287 A US201815913287 A US 201815913287A US 2018193830 A1 US2018193830 A1 US 2018193830A1
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- Prior art keywords
- section
- container
- reagent
- hole
- sealing layer
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/16—Reagents, handling or storing thereof
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/042—Caps; Plugs
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/041—Connecting closures to device or container
- B01L2300/044—Connecting closures to device or container pierceable, e.g. films, membranes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/04—Closures and closing means
- B01L2300/046—Function or devices integrated in the closure
- B01L2300/047—Additional chamber, reservoir
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0672—Integrated piercing tool
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
- B01L2400/0683—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers mechanically breaking a wall or membrane within a channel or chamber
Definitions
- the present invention relates to biopsy sample containers.
- a known biopsy sample container contains a biological sample within a first container having an opening at one end thereof and contains a reagent within a second container sealed by means of a pierceable film.
- the film is pierced by means of a protrusion provided in the first container so that the reagent within the second container can be fed to the biological sample within the first container (for example, see PCT International Publication No. WO 2008/152980).
- An aspect of the present disclosure is a biopsy sample container including: a sample containing section capable of containing a biological sample; a reagent containing section capable of containing a reagent; a sealing layer that separates the reagent containing section and the sample containing section from each other in a liquid-tight state; a seal-breaking section that forms a through-hole in the sealing layer by movement relative to the sealing layer; and a resealing section that closes the through-hole formed by the seal-breaking section.
- FIG. 1 is a cross-sectional view illustrating a state where a reagent containing section and a sample containing section are separated from each other in a biopsy sample container according to an embodiment of the present invention.
- FIG. 2 is a longitudinal sectional view illustrating the biopsy sample container in FIG. 1 .
- FIG. 3 is a cross-sectional view illustrating a state where a through-hole is formed in a film member by means of a protrusion of the biopsy sample container in FIG. 1 .
- FIG. 4 is a vertical sectional view illustrating the biopsy sample container in FIG. 3 .
- FIG. 5 is a cross-sectional view illustrating a state where a reagent is flowing through the through-hole formed in the film member of the biopsy sample container in FIG. 1 .
- FIG. 6A is a partially enlarged view illustrating a modification of the protrusion of the biopsy sample container in FIG. 1 .
- FIG. 6B is a partially enlarged view illustrating a state where a through-hole is formed in the film member by means of the protrusion in FIG. 6A .
- FIG. 6C is a partially enlarged view illustrating a state where the through-hole formed in FIG. 6B is closed by a plate-shaped section.
- FIG. 7 is a vertical sectional view illustrating an example where a seal member is disposed in the plate-shaped section of the biopsy sample container in FIG. 6A .
- FIG. 8 is a partially enlarged view illustrating a modification of the protrusion of the biopsy sample container in FIG. 6A .
- FIG. 9A is a vertical sectional view illustrating a modification of the biopsy sample container in FIG. 1 .
- FIG. 9B is a vertical sectional view illustrating a state where a through-hole is formed in the film member by means of the protrusion of the biopsy sample container in FIG. 9 A.
- FIG. 9C is a vertical sectional view illustrating a state where the through-hole formed in FIG. 9B is closed by a flat surface section.
- FIG. 10A is a vertical sectional view illustrating another modification of the biopsy sample container in FIG. 1 .
- FIG. 10B is a vertical sectional view illustrating a state where a through-hole is formed in the film member by means of the protrusion of the biopsy sample container in FIG. 10A .
- FIG. 10C is a vertical sectional view illustrating a state where the through-hole formed in FIG. 10B is closed by the flat surface section.
- a biopsy sample container 1 according to an embodiment of the present invention will be described below with reference to the drawings.
- a biological sample A may include any material derived from a biological organism and may refer to, for example, solid samples such as excrement, nails, hair, or tissues, or liquid samples such as blood, sputum, saliva, or urine.
- a reagent B may be an organic solvent or a solution containing an organic solvent.
- the organic solvent used may be, for example, phenol or chloroform. It is more preferable to employ a reagent B containing a water-soluble organic solvent as an active ingredient.
- the biopsy sample container 1 includes a sample container (second container) 2 having a sample containing section 2 a that contains the biological sample A and a reagent container (first container) 3 having a reagent containing section 3 a that contains the reagent B.
- the sample container 2 is a bottomed cylinder with one end of which has an opening 2 b and the other end of which is closed by a base 2 c.
- a male thread 2 d is formed on the outer peripheral surface near the opening 2 b of the sample container 2 .
- a ring-shaped member 5 having a protrusion 4 is fixed to the end at the opening 2 b side of the sample container 2 .
- the ring-shaped member 5 includes a plate-shaped section (contact member, resealing section) 6 protruding radially inward and extending in the axial direction.
- One surface of the plate-shaped section 6 is provided with the protrusion (seal-breaking section) 4 .
- the protrusion 4 gradually tapers from the plate-shaped section 6 toward a pointed end 4 a.
- a seal member 8 such as an O-ring, is disposed on the outer peripheral surface of the ring-shaped member 5 so as to seal the gap between the outer peripheral surface and the inner surface of a cap member 7 , which will be described below.
- the reagent container 3 is integrally provided with the cap member 7 , which is cylindrical, so as to protrude inward in the axial direction from an end surface of the cap member 7 having a female thread 7 a to be fastened to the male thread 2 d of the sample container 2 .
- a surface of the reagent container 3 extending in the axial direction is provided with an opening 3 b .
- the opening 3 b is closed in a liquid-tight state by a thin film member (sealing layer) 9 . Accordingly, the reagent B contained within the reagent container 3 is maintained in the contained state within the reagent container 3 unless the film member 9 breaks.
- the cap member 7 provided with the reagent container 3 By using the cap member 7 provided with the reagent container 3 to cover so as to close the opening 2 b of the sample container 2 , the reagent container 3 is inserted into the sample container 2 .
- the protrusion 4 provided on the plate-shaped section 6 of the ring-shaped member 5 is disposed at a position where it opposes, in the circumferential direction, the film member 9 closing the opening 3 b of the reagent container 3 .
- the gap between the inner surface of the cap member 7 and the outer surface of the ring-shaped member 5 is hermetically sealed by the seal member 8 interposed therebetween, so that the opening 2 b of the sample container 2 is hermetically sealed to the outside.
- the cap member 7 When the female thread 7 a of the cap member 7 is fastened to the male thread 2 d of the sample container 2 in this state, the cap member 7 is rotated relative to the sample container 2 about the axis of the sample container 2 .
- the reagent container 3 provided integrally with the cap member 7 moves in the circumferential direction when the cap member 7 is rotated relative to the sample container 2 , thus causing the protrusion 4 to move closer toward the film member 9 . Consequently, as the cap member 7 is rotated relative to the sample container 2 , the protrusion 4 comes into contact with the film member 9 and forms a through-hole 10 therein.
- the plate-shaped section 6 disposed at the root of the protrusion 4 is brought into close contact with the surface of the film member 9 .
- the cap member 7 is provided with a supply hole 11 for supplying the biological sample A into the sample container 2 in the state where the opening 2 b is closed by the cap member 7 .
- the supply hole 11 has a diameter that allows an injection needle of a syringe containing the biological sample A to penetrate therethrough.
- the ring-shaped member 5 is provided with a closing plate 12 that closes the supply hole 11 at the time when the protrusion 4 penetrates the film member 9 .
- the closing plate 12 has the shape of a flat plate that protrudes radially inward from near an end of the ring-shaped member 5 .
- the cap member 7 is rotated relative to the sample container 2 so as to close the supply hole 11 near a relative position where the protrusion 4 reaches the film member 9 , thereby shutting off the sample container 2 in a sealed state from the outside.
- the reagent B is contained within the reagent containing section 3 a of the reagent container 3 provided in the cap member 7 , and the opening 3 b is sealed by using the film member 9 .
- the opening 2 b of the sample container 2 is closed.
- the cap member 7 is used to keep the sample container 2 in a sealed state by engaging a part of the female thread 7 a of the cap member 7 with a part of the male thread 2 d of the sample container 2 .
- the protrusion 4 and the film member 9 are disposed at positions separated from each other in the circumferential direction.
- the biological sample A is fed into the sample container 2 in the state shown in FIGS. 1 and 2 via the supply hole 11 of the cap member 7 . Because the supply hole 11 is open, the biological sample A can be readily contained within the sample containing section 2 a of the sample container 2 .
- an operator rotates the cap member 7 relative to the sample container 2 about the axis of the sample container 2 .
- This causes the female thread 7 a of the cap member 7 to be further fastened to the male thread 2 d of the sample container 2 so that the cap member 7 is brought close to be drawn in the axial direction with respect to the sample container 2 .
- the reagent container 3 provided in the cap member 7 is moved in the circumferential direction so as to be brought close to the protrusion 4 of the ring-shaped member 5 .
- the protrusion 4 provided in the ring-shaped member 5 forms the through-hole 10 in the film member 9 closing the opening 3 b of the reagent container 3 , as shown in FIG. 5 .
- the reagent B sealed within the reagent container 3 flows out into the sample container 2 via the through-hole 10 in the film member 9 , as indicated by the arrows, so that the reagent B can be fed to and mixed with the biological sample A contained within the sample container 2 .
- the closing plate 12 provided in the ring-shaped member 5 closes the supply hole 11 of the cap member 7 so as to shut off the internal space of the biopsy sample container 1 from the external space.
- the operator can mix the reagent B and the biological sample A together without touching them or without aspirating volatile substance.
- the biopsy sample container 1 is advantageous in that the operator can mix the biological sample A and the reagent B together in the sealed biopsy sample container 1 by simply injecting the collected biological sample A into the sample container 2 through the supply hole 11 by using a syringe and then rotating the cap member 7 relative to the sample container 2 .
- the protrusion 4 may be inclined in one direction toward the pointed end 4 a , as shown in FIGS. 6A to 6C .
- the protrusion 4 has a recess 4 b formed on the proximal end thereof relative to the pointed end 4 a.
- the reagent B within the reagent container 3 can readily flow toward the sample container 2 , as indicated by an arrow, so that the reagent B can be mixed with the biological sample A more quickly.
- the plate-shaped section 6 is brought further closer to the film member 9 , the plate-shaped section 6 is brought into close contact with the surface of the film member 9 around the entire periphery surrounding the through-hole 10 , as shown in FIG. 6C , whereby the through-hole 10 becomes closed by the plate-shaped section 6 .
- the mixture of the reagent B and the biological sample A can be prevented from backflow into the reagent container 3 .
- the process for formation of the through-hole 10 in the film member 9 and the process for release of the reagent B as well as prevention of the reagent B from backflow can be sequentially performed by simply moving the plate-shaped section 6 provided with the protrusion 4 in one direction relative to the film member 9 .
- a seal member 13 such as an O-ring, may be disposed at a position surrounding the protrusion 4 on the surface of the plate-shaped section 6 . Accordingly, instead of bringing the plate-shaped section 6 into contact with the surface of the film member 9 , the seal member 13 is brought into close contact with the entire periphery around the through-hole 10 so that the through-hole 10 can be closed more reliably.
- the protrusion 4 may include a recess 4 b , which is depressed in a direction intersecting the longitudinal direction, at the proximal end of the protrusion 4 that gradually tapers toward the pointed end 4 a , as shown in FIG. 8 . Accordingly, after the protrusion 4 forms the through-hole 10 in the film member 9 and the part of the protrusion 4 with the maximum diameter passes through the film member 9 , the film member 9 is disposed at the position of the recess 4 b , so that the gap between the protrusion 4 and the through-hole 10 is increased, whereby the reagent B can readily flow out therethrough.
- the reagent container 3 is rotated relative to the sample container 2 about the longitudinal axis of the sample container 2 so that both containers 2 and 3 are moved relative to each other.
- the reagent container 3 may be moved relative to the sample container 2 in the longitudinal direction of the sample container 2 .
- the reagent B is contained in the reagent container 3 and is sealed therein by the film member 9 , and a connection member 16 having the protrusion 4 , a flat surface section (contact member, resealing section) 14 , and an inlet 15 toward the sample container 2 is fixed to the sample container 2 .
- a connection member 16 having the protrusion 4 , a flat surface section (contact member, resealing section) 14 , and an inlet 15 toward the sample container 2 is fixed to the sample container 2 .
- FIG. 9A after the biological sample A is contained within the sample container 2 , the sample container 2 and the reagent container 3 are connected to each other by means of the connection member 16 in a state where they are shut off from the outside. Then, as shown in FIG. 9B , the sample container 2 and the reagent container 3 are relatively brought close to each other.
- the through-hole 10 is formed in the film member 9 by the protrusion 4 , so that the reagent B within the reagent container 3 is caused to flow into the sample container 2 via the through-hole 10 and the inlet 15 , as indicated by arrows, thereby facilitating the mixing process.
- the sample container 2 and the reagent container 3 are brought close to each other as much as possible, so that the flat surface section 14 provided at the proximal end of the protrusion 4 is brought into close contact with the surface of the film member 9 , whereby the mixture of the biological sample A and the reagent B can be prevented from backflow into the reagent container 3 .
- the protrusion 4 and the flat surface section 14 may be configured to be movable relative to the film member 9 that separates the reagent containing section 3 a and the sample containing section 2 a , which are fixed to each other, from each other in a liquid-tight state, as shown in FIGS. 10A to 10C .
- FIG. 10B after forming the through-hole 10 in the film member 9 by using the protrusion 4 and supplying the reagent B to the biological sample A, the flat surface section 14 may be brought into close contact with the surface of the film member 9 so as to close the through-hole 10 .
- a syringe having the reagent containing section 3 a as a cylinder and the flat surface section 14 as a piston may be configured, and the reagent B may be pushed out from the through-hole 10 by the movement of the flat surface section 14 .
- the number and shape of the protrusions 4 are not limited, and the through-hole 10 may be formed in the film member 9 by means of an arbitrary number of protrusions 4 .
- An aspect of the present disclosure is a biopsy sample container including: a sample containing section capable of containing a biological sample; a reagent containing section capable of containing a reagent; a sealing layer that separates the reagent containing section and the sample containing section from each other in a liquid-tight state; a seal-breaking section that forms a through-hole in the sealing layer by movement relative to the sealing layer; and a resealing section that closes the through-hole formed by the seal-breaking section.
- the reagent is contained in the reagent containing section
- the biological sample is contained in the sample containing section separated from the reagent within the reagent containing section by the sealing layer
- the seal-breaking section is moved relative to the sealing layer, so that the through-hole is formed in the sealing layer by the seal-breaking section.
- the reagent contained in the reagent containing section flows into the sample containing section via the through-hole so as to be mixed with the biological sample.
- the resealing section closes the through-hole so that the mixture of the reagent and the biological sample is prevented from backflow into the reagent containing section via the through-hole, thereby reducing a loss of the valuable biological sample.
- the reagent containing section may be provided in a first container having an opening sealed by the sealing layer, the sample containing section may be provided in a second container having the seal-breaking section and the resealing section, and the second container and the first container may be connectable in a relatively movable manner.
- the first container including the reagent containing section containing the reagent and the second container including the sample containing section containing the biological sample are connected and are moved relative to each other, so as to bring the seal-breaking section close to the sealing layer, which seals the opening of the reagent containing section, and thus form the through-hole in the sealing layer, whereby the reagent and the biological sample can be mixed together.
- the resealing section provided in the second container is brought close to the through-hole so as to close the through-hole, whereby the mixture of the reagent and the biological sample can be prevented from backflow into the reagent containing section.
- the sealing layer may be a film member disposed so as to close the opening of the first container
- the seal-breaking section may be a protrusion capable of piercing the film member
- the resealing section may be a contact member that is brought into close contact with a surface of the film member throughout the entire periphery surrounding the through-hole.
- the through-hole is formed in the film member, which closes the reagent containing section, by means of the protrusion provided in the second container, thus causing the reagent to flow into the sample containing section in which the biological sample is contained.
- the contact member provided in the second container is brought into close contact with the surface of the film member surrounding the through-hole, thereby closing the through-hole and preventing the mixture from backflow into the reagent containing section.
- the seal-breaking section may include a pointed end that forms the through-hole in the sealing layer and a recess that is disposed closer to a proximal end relative to the pointed end and that is depressed in a direction intersecting a direction in which the seal-breaking section moves relative to the sealing layer.
- the sealing layer and the seal-breaking section are moved relative to each other so as to form the through-hole in the sealing layer by means of the pointed end of the seal-breaking section. Then, the sealing layer and the seal-breaking section are further moved relative to each other in the same direction so that the recess is aligned with the through-hole, thereby increasing the opening area of the through-hole and allowing the reagent to flow out readily from the reagent containing section.
- the aforementioned aspects afford an advantageous effect in that an operator can mix a reagent and a biological sample together without touching them or without aspirating volatile substances and in that a loss of the biological sample can be reduced.
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- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
Description
- This is a Continuation Application of International Application No. PCT/JP2015/076844 filed on Sep. 24, 2015, the content of which is hereby incorporated by reference herein in its entirety.
- The present invention relates to biopsy sample containers.
- A known biopsy sample container contains a biological sample within a first container having an opening at one end thereof and contains a reagent within a second container sealed by means of a pierceable film. When connecting the two containers together, the film is pierced by means of a protrusion provided in the first container so that the reagent within the second container can be fed to the biological sample within the first container (for example, see PCT International Publication No. WO 2008/152980).
- An aspect of the present disclosure is a biopsy sample container including: a sample containing section capable of containing a biological sample; a reagent containing section capable of containing a reagent; a sealing layer that separates the reagent containing section and the sample containing section from each other in a liquid-tight state; a seal-breaking section that forms a through-hole in the sealing layer by movement relative to the sealing layer; and a resealing section that closes the through-hole formed by the seal-breaking section.
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FIG. 1 is a cross-sectional view illustrating a state where a reagent containing section and a sample containing section are separated from each other in a biopsy sample container according to an embodiment of the present invention. -
FIG. 2 is a longitudinal sectional view illustrating the biopsy sample container inFIG. 1 . -
FIG. 3 is a cross-sectional view illustrating a state where a through-hole is formed in a film member by means of a protrusion of the biopsy sample container inFIG. 1 . -
FIG. 4 is a vertical sectional view illustrating the biopsy sample container inFIG. 3 . -
FIG. 5 is a cross-sectional view illustrating a state where a reagent is flowing through the through-hole formed in the film member of the biopsy sample container inFIG. 1 . -
FIG. 6A is a partially enlarged view illustrating a modification of the protrusion of the biopsy sample container inFIG. 1 . -
FIG. 6B is a partially enlarged view illustrating a state where a through-hole is formed in the film member by means of the protrusion inFIG. 6A . -
FIG. 6C is a partially enlarged view illustrating a state where the through-hole formed inFIG. 6B is closed by a plate-shaped section. -
FIG. 7 is a vertical sectional view illustrating an example where a seal member is disposed in the plate-shaped section of the biopsy sample container inFIG. 6A . -
FIG. 8 is a partially enlarged view illustrating a modification of the protrusion of the biopsy sample container inFIG. 6A . -
FIG. 9A is a vertical sectional view illustrating a modification of the biopsy sample container inFIG. 1 . -
FIG. 9B is a vertical sectional view illustrating a state where a through-hole is formed in the film member by means of the protrusion of the biopsy sample container in FIG. 9A. -
FIG. 9C is a vertical sectional view illustrating a state where the through-hole formed inFIG. 9B is closed by a flat surface section. -
FIG. 10A is a vertical sectional view illustrating another modification of the biopsy sample container inFIG. 1 . -
FIG. 10B is a vertical sectional view illustrating a state where a through-hole is formed in the film member by means of the protrusion of the biopsy sample container inFIG. 10A . -
FIG. 10C is a vertical sectional view illustrating a state where the through-hole formed inFIG. 10B is closed by the flat surface section. - A biopsy sample container 1 according to an embodiment of the present invention will be described below with reference to the drawings.
- Before proceeding with the description of the embodiment, a biological sample A may include any material derived from a biological organism and may refer to, for example, solid samples such as excrement, nails, hair, or tissues, or liquid samples such as blood, sputum, saliva, or urine. A reagent B may be an organic solvent or a solution containing an organic solvent. The organic solvent used may be, for example, phenol or chloroform. It is more preferable to employ a reagent B containing a water-soluble organic solvent as an active ingredient.
- As shown in
FIGS. 1 to 4 , the biopsy sample container 1 according to this embodiment includes a sample container (second container) 2 having asample containing section 2 a that contains the biological sample A and a reagent container (first container) 3 having areagent containing section 3 a that contains the reagent B. - The
sample container 2 is a bottomed cylinder with one end of which has an opening 2 b and the other end of which is closed by abase 2 c. - A
male thread 2 d is formed on the outer peripheral surface near the opening 2 b of thesample container 2. A ring-shaped member 5 having aprotrusion 4 is fixed to the end at the opening 2 b side of thesample container 2. The ring-shaped member 5 includes a plate-shaped section (contact member, resealing section) 6 protruding radially inward and extending in the axial direction. One surface of the plate-shaped section 6 is provided with the protrusion (seal-breaking section) 4. Theprotrusion 4 gradually tapers from the plate-shaped section 6 toward apointed end 4 a. - A
seal member 8, such as an O-ring, is disposed on the outer peripheral surface of the ring-shaped member 5 so as to seal the gap between the outer peripheral surface and the inner surface of acap member 7, which will be described below. - The
reagent container 3 is integrally provided with thecap member 7, which is cylindrical, so as to protrude inward in the axial direction from an end surface of thecap member 7 having afemale thread 7 a to be fastened to themale thread 2 d of thesample container 2. A surface of thereagent container 3 extending in the axial direction is provided with anopening 3 b. The opening 3 b is closed in a liquid-tight state by a thin film member (sealing layer) 9. Accordingly, the reagent B contained within thereagent container 3 is maintained in the contained state within thereagent container 3 unless thefilm member 9 breaks. - By using the
cap member 7 provided with thereagent container 3 to cover so as to close theopening 2 b of thesample container 2, thereagent container 3 is inserted into thesample container 2. Thus, theprotrusion 4 provided on the plate-shaped section 6 of the ring-shaped member 5 is disposed at a position where it opposes, in the circumferential direction, thefilm member 9 closing the opening 3 b of thereagent container 3. - Furthermore, the gap between the inner surface of the
cap member 7 and the outer surface of the ring-shapedmember 5 is hermetically sealed by theseal member 8 interposed therebetween, so that theopening 2 b of thesample container 2 is hermetically sealed to the outside. - When the
female thread 7 a of thecap member 7 is fastened to themale thread 2 d of thesample container 2 in this state, thecap member 7 is rotated relative to thesample container 2 about the axis of thesample container 2. - Because the ring-shaped
member 5 is fixed to thesample container 2, thereagent container 3 provided integrally with thecap member 7 moves in the circumferential direction when thecap member 7 is rotated relative to thesample container 2, thus causing theprotrusion 4 to move closer toward thefilm member 9. Consequently, as thecap member 7 is rotated relative to thesample container 2, theprotrusion 4 comes into contact with thefilm member 9 and forms a through-hole 10 therein. - As the
cap member 7 is further rotated relative to thesample container 2 from the state where the through-hole 10 is formed, the plate-shapedsection 6 disposed at the root of theprotrusion 4 is brought into close contact with the surface of thefilm member 9. - Moreover, the
cap member 7 is provided with asupply hole 11 for supplying the biological sample A into thesample container 2 in the state where theopening 2 b is closed by thecap member 7. For example, thesupply hole 11 has a diameter that allows an injection needle of a syringe containing the biological sample A to penetrate therethrough. - Furthermore, the ring-shaped
member 5 is provided with aclosing plate 12 that closes thesupply hole 11 at the time when theprotrusion 4 penetrates thefilm member 9. The closingplate 12 has the shape of a flat plate that protrudes radially inward from near an end of the ring-shapedmember 5. Thecap member 7 is rotated relative to thesample container 2 so as to close thesupply hole 11 near a relative position where theprotrusion 4 reaches thefilm member 9, thereby shutting off thesample container 2 in a sealed state from the outside. - The operation of the biopsy sample container 1 according to this embodiment having the above-described configuration will be described below.
- In order to mix the biological sample A and the reagent B using the biopsy sample container 1 according to this embodiment, the reagent B is contained within the
reagent containing section 3 a of thereagent container 3 provided in thecap member 7, and theopening 3 b is sealed by using thefilm member 9. By using thecap member 7 in this state, theopening 2 b of thesample container 2 is closed. - Specifically, as shown in
FIG. 2 , thecap member 7 is used to keep thesample container 2 in a sealed state by engaging a part of thefemale thread 7 a of thecap member 7 with a part of themale thread 2 d of thesample container 2. In this state, as shown inFIG. 1 , theprotrusion 4 and thefilm member 9 are disposed at positions separated from each other in the circumferential direction. - The biological sample A is fed into the
sample container 2 in the state shown inFIGS. 1 and 2 via thesupply hole 11 of thecap member 7. Because thesupply hole 11 is open, the biological sample A can be readily contained within thesample containing section 2 a of thesample container 2. - In this state, an operator rotates the
cap member 7 relative to thesample container 2 about the axis of thesample container 2. This causes thefemale thread 7 a of thecap member 7 to be further fastened to themale thread 2 d of thesample container 2 so that thecap member 7 is brought close to be drawn in the axial direction with respect to thesample container 2. Moreover, thereagent container 3 provided in thecap member 7 is moved in the circumferential direction so as to be brought close to theprotrusion 4 of the ring-shapedmember 5. - Then, when the
cap member 7 is rotated relative to thesample container 2 by a predetermined angle, theprotrusion 4 provided in the ring-shapedmember 5 forms the through-hole 10 in thefilm member 9 closing theopening 3 b of thereagent container 3, as shown inFIG. 5 . As a result, the reagent B sealed within thereagent container 3 flows out into thesample container 2 via the through-hole 10 in thefilm member 9, as indicated by the arrows, so that the reagent B can be fed to and mixed with the biological sample A contained within thesample container 2. - As shown in
FIG. 5 , at this point, the closingplate 12 provided in the ring-shapedmember 5 closes thesupply hole 11 of thecap member 7 so as to shut off the internal space of the biopsy sample container 1 from the external space. - Accordingly, the operator can mix the reagent B and the biological sample A together without touching them or without aspirating volatile substance.
- Furthermore, as shown in
FIGS. 3 and 4 , when the operator rotates thecap member 7 relative to thesample container 2 in the same direction about the axis of thesample container 2, the plate-shapedsection 6 comes into close contact with the surface of thefilm member 9 so that the through-hole 10 formed in thefilm member 9 is closed again by the plate-shapedsection 6. Thus, the mixture of the reagent B and the biological sample A mixed within thesample container 2 can be prevented from backflow into thereagent container 3 via the through-hole 10 formed in thefilm member 9. - Accordingly, the biopsy sample container 1 according to this embodiment is advantageous in that the operator can mix the biological sample A and the reagent B together in the sealed biopsy sample container 1 by simply injecting the collected biological sample A into the
sample container 2 through thesupply hole 11 by using a syringe and then rotating thecap member 7 relative to thesample container 2. - As an alternative to this embodiment in which the
protrusion 4 used gradually tapers toward thepointed end 4 a, theprotrusion 4 may be inclined in one direction toward thepointed end 4 a, as shown inFIGS. 6A to 6C . Thus, theprotrusion 4 has arecess 4 b formed on the proximal end thereof relative to thepointed end 4 a. - Accordingly, when the
protrusion 4 forms the through-hole 10 in thefilm member 9, as shown inFIG. 6B , while the plate-shapedsection 6 is brought close to thefilm member 9, as shown inFIG. 6A , the position where theprotrusion 4 pierces thefilm member 9 is gradually shifted along the surface of thefilm member 9 so that the gap between the through-hole 10 and theprotrusion 4 is widened. - As a result, the reagent B within the
reagent container 3 can readily flow toward thesample container 2, as indicated by an arrow, so that the reagent B can be mixed with the biological sample A more quickly. Then, when the plate-shapedsection 6 is brought further closer to thefilm member 9, the plate-shapedsection 6 is brought into close contact with the surface of thefilm member 9 around the entire periphery surrounding the through-hole 10, as shown inFIG. 6C , whereby the through-hole 10 becomes closed by the plate-shapedsection 6. - Accordingly, the mixture of the reagent B and the biological sample A can be prevented from backflow into the
reagent container 3. - Specifically, by using such a
protrusion 4, the process for formation of the through-hole 10 in thefilm member 9 and the process for release of the reagent B as well as prevention of the reagent B from backflow can be sequentially performed by simply moving the plate-shapedsection 6 provided with theprotrusion 4 in one direction relative to thefilm member 9. - Furthermore, as shown in
FIG. 7 , aseal member 13, such as an O-ring, may be disposed at a position surrounding theprotrusion 4 on the surface of the plate-shapedsection 6. Accordingly, instead of bringing the plate-shapedsection 6 into contact with the surface of thefilm member 9, theseal member 13 is brought into close contact with the entire periphery around the through-hole 10 so that the through-hole 10 can be closed more reliably. - As an alternative to the
protrusion 4 that is inclined in one direction, theprotrusion 4 may include arecess 4 b, which is depressed in a direction intersecting the longitudinal direction, at the proximal end of theprotrusion 4 that gradually tapers toward thepointed end 4 a, as shown inFIG. 8 . Accordingly, after theprotrusion 4 forms the through-hole 10 in thefilm member 9 and the part of theprotrusion 4 with the maximum diameter passes through thefilm member 9, thefilm member 9 is disposed at the position of therecess 4 b, so that the gap between theprotrusion 4 and the through-hole 10 is increased, whereby the reagent B can readily flow out therethrough. - Furthermore, in this embodiment, the
reagent container 3 is rotated relative to thesample container 2 about the longitudinal axis of thesample container 2 so that bothcontainers FIGS. 9A to 9C , thereagent container 3 may be moved relative to thesample container 2 in the longitudinal direction of thesample container 2. - Specifically, the reagent B is contained in the
reagent container 3 and is sealed therein by thefilm member 9, and aconnection member 16 having theprotrusion 4, a flat surface section (contact member, resealing section) 14, and aninlet 15 toward thesample container 2 is fixed to thesample container 2. As shown inFIG. 9A , after the biological sample A is contained within thesample container 2, thesample container 2 and thereagent container 3 are connected to each other by means of theconnection member 16 in a state where they are shut off from the outside. Then, as shown inFIG. 9B , thesample container 2 and thereagent container 3 are relatively brought close to each other. - Accordingly, the through-
hole 10 is formed in thefilm member 9 by theprotrusion 4, so that the reagent B within thereagent container 3 is caused to flow into thesample container 2 via the through-hole 10 and theinlet 15, as indicated by arrows, thereby facilitating the mixing process. - Subsequently, as shown in
FIG. 9C , thesample container 2 and thereagent container 3 are brought close to each other as much as possible, so that theflat surface section 14 provided at the proximal end of theprotrusion 4 is brought into close contact with the surface of thefilm member 9, whereby the mixture of the biological sample A and the reagent B can be prevented from backflow into thereagent container 3. - Furthermore, instead of connecting the
sample container 2 and thereagent container 3 in a relatively movable manner, theprotrusion 4 and theflat surface section 14 may be configured to be movable relative to thefilm member 9 that separates thereagent containing section 3 a and thesample containing section 2 a, which are fixed to each other, from each other in a liquid-tight state, as shown inFIGS. 10A to 10C . As shown inFIG. 10B , after forming the through-hole 10 in thefilm member 9 by using theprotrusion 4 and supplying the reagent B to the biological sample A, theflat surface section 14 may be brought into close contact with the surface of thefilm member 9 so as to close the through-hole 10. - In this case, a syringe having the
reagent containing section 3 a as a cylinder and theflat surface section 14 as a piston may be configured, and the reagent B may be pushed out from the through-hole 10 by the movement of theflat surface section 14. - The number and shape of the
protrusions 4 are not limited, and the through-hole 10 may be formed in thefilm member 9 by means of an arbitrary number ofprotrusions 4. - From the above-described embodiments, the following aspects of the present invention are derived.
- An aspect of the present disclosure is a biopsy sample container including: a sample containing section capable of containing a biological sample; a reagent containing section capable of containing a reagent; a sealing layer that separates the reagent containing section and the sample containing section from each other in a liquid-tight state; a seal-breaking section that forms a through-hole in the sealing layer by movement relative to the sealing layer; and a resealing section that closes the through-hole formed by the seal-breaking section.
- According to this aspect, the reagent is contained in the reagent containing section, the biological sample is contained in the sample containing section separated from the reagent within the reagent containing section by the sealing layer, and the seal-breaking section is moved relative to the sealing layer, so that the through-hole is formed in the sealing layer by the seal-breaking section. The reagent contained in the reagent containing section flows into the sample containing section via the through-hole so as to be mixed with the biological sample.
- After the reagent and the biological sample are mixed together, the resealing section closes the through-hole so that the mixture of the reagent and the biological sample is prevented from backflow into the reagent containing section via the through-hole, thereby reducing a loss of the valuable biological sample.
- In the above aspect, the reagent containing section may be provided in a first container having an opening sealed by the sealing layer, the sample containing section may be provided in a second container having the seal-breaking section and the resealing section, and the second container and the first container may be connectable in a relatively movable manner.
- Accordingly, the first container including the reagent containing section containing the reagent and the second container including the sample containing section containing the biological sample are connected and are moved relative to each other, so as to bring the seal-breaking section close to the sealing layer, which seals the opening of the reagent containing section, and thus form the through-hole in the sealing layer, whereby the reagent and the biological sample can be mixed together. Moreover, by moving the first container and the second container relative to each other, the resealing section provided in the second container is brought close to the through-hole so as to close the through-hole, whereby the mixture of the reagent and the biological sample can be prevented from backflow into the reagent containing section.
- Furthermore, in the above aspect, the sealing layer may be a film member disposed so as to close the opening of the first container, the seal-breaking section may be a protrusion capable of piercing the film member, and the resealing section may be a contact member that is brought into close contact with a surface of the film member throughout the entire periphery surrounding the through-hole.
- Accordingly, by moving the first container and the second container relative to each other, the through-hole is formed in the film member, which closes the reagent containing section, by means of the protrusion provided in the second container, thus causing the reagent to flow into the sample containing section in which the biological sample is contained. Moreover, by further moving the first container and the second container relative to each other, the contact member provided in the second container is brought into close contact with the surface of the film member surrounding the through-hole, thereby closing the through-hole and preventing the mixture from backflow into the reagent containing section.
- Furthermore, the seal-breaking section may include a pointed end that forms the through-hole in the sealing layer and a recess that is disposed closer to a proximal end relative to the pointed end and that is depressed in a direction intersecting a direction in which the seal-breaking section moves relative to the sealing layer.
- Accordingly, the sealing layer and the seal-breaking section are moved relative to each other so as to form the through-hole in the sealing layer by means of the pointed end of the seal-breaking section. Then, the sealing layer and the seal-breaking section are further moved relative to each other in the same direction so that the recess is aligned with the through-hole, thereby increasing the opening area of the through-hole and allowing the reagent to flow out readily from the reagent containing section.
- The aforementioned aspects afford an advantageous effect in that an operator can mix a reagent and a biological sample together without touching them or without aspirating volatile substances and in that a loss of the biological sample can be reduced.
-
- A biological sample
- B reagent
- 1 biopsy sample container
- 2 sample container (second container)
- 2 a sample containing section
- 3 reagent container (first container)
- 3 a reagent containing section
- 3 b opening
- 4 protrusion (sealing-breaking section)
- 4 a pointed end
- 4 b recess
- 6 plate-shaped section (contact member, resealing section)
- 9 film member (sealing layer)
- 10 through-hole
- 14 flat surface section (contact member, resealing section)
Claims (4)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP2015/076844 WO2017051448A1 (en) | 2015-09-24 | 2015-09-24 | Biopsy specimen container |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2015/076844 Continuation WO2017051448A1 (en) | 2015-09-24 | 2015-09-24 | Biopsy specimen container |
Publications (1)
Publication Number | Publication Date |
---|---|
US20180193830A1 true US20180193830A1 (en) | 2018-07-12 |
Family
ID=58386303
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US15/913,287 Abandoned US20180193830A1 (en) | 2015-09-24 | 2018-03-06 | Biopsy sample container |
Country Status (3)
Country | Link |
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US (1) | US20180193830A1 (en) |
JP (1) | JPWO2017051448A1 (en) |
WO (1) | WO2017051448A1 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020201250A1 (en) * | 2019-04-02 | 2020-10-08 | Anvajo GmbH | Device and method for taking up and handling a liquid sample and a substance |
US20230095562A1 (en) * | 2021-09-29 | 2023-03-30 | James Frankland ZERO | Specimen collection receptacle |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
RU176704U1 (en) * | 2017-09-21 | 2018-01-25 | Егор Евгеньевич Круглов | CONTAINER FOR TRANSPORTATION IN ISOTHERMAL CONDITIONS AND HOMOGENIZATION OF BIOPSI MATERIAL FOR MICROBIOLOGICAL STUDIES |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3715189A (en) * | 1970-06-15 | 1973-02-06 | Secretary Of The Treasury | Qualitative analysis device |
US6641782B1 (en) * | 2000-11-15 | 2003-11-04 | Polaroid Corporation | Apparatus for performing diagnostic testing |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP3519831B2 (en) * | 1995-09-04 | 2004-04-19 | 積水化学工業株式会社 | Fecal occult blood determination device |
JPWO2008152980A1 (en) * | 2007-06-12 | 2010-08-26 | オリンパス株式会社 | Container for mixing biological sample and reagent and method for mixing biological sample and reagent |
SE531873C2 (en) * | 2007-11-12 | 2009-09-01 | Lifeassays Ab | Device for biochemical processing and analysis of sample liquid |
JP2010008106A (en) * | 2008-06-24 | 2010-01-14 | Olympus Corp | Feces treatment container and method |
-
2015
- 2015-09-24 JP JP2017541187A patent/JPWO2017051448A1/en active Pending
- 2015-09-24 WO PCT/JP2015/076844 patent/WO2017051448A1/en active Application Filing
-
2018
- 2018-03-06 US US15/913,287 patent/US20180193830A1/en not_active Abandoned
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3715189A (en) * | 1970-06-15 | 1973-02-06 | Secretary Of The Treasury | Qualitative analysis device |
US6641782B1 (en) * | 2000-11-15 | 2003-11-04 | Polaroid Corporation | Apparatus for performing diagnostic testing |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2020201250A1 (en) * | 2019-04-02 | 2020-10-08 | Anvajo GmbH | Device and method for taking up and handling a liquid sample and a substance |
US20230095562A1 (en) * | 2021-09-29 | 2023-03-30 | James Frankland ZERO | Specimen collection receptacle |
US11919005B2 (en) * | 2021-09-29 | 2024-03-05 | James Frankland ZERO | Specimen collection receptacle |
Also Published As
Publication number | Publication date |
---|---|
JPWO2017051448A1 (en) | 2018-07-26 |
WO2017051448A1 (en) | 2017-03-30 |
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